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Fig. 8.

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Saturation curves for ANG-(1–7) and ANG II metabolism by PCMB-sensitive enzyme in CSF. Pooled CSF from control or BMX sheep was concentrated by a 50-kDa filtration tube. ○, With solid lines, control animals; ●, with dashed lines, BMX animals. Km and Vmax for ANG-(1–7) metabolism were calculated with a GraphPad Prism 5 statistical program. For control animals Km = 5.4 μM ANG-(1–7); Vmax = 54.1 nmol·min−1·mg protein−1. For BMX animals Km = 4.1 μM ANG-(1–7); Vmax = 56.9 nmol·min−1·mg protein−1.

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