Nothing Special   »   [go: up one dir, main page]

Europe PMC requires Javascript to function effectively.

Either your web browser doesn't support Javascript or it is currently turned off. In the latter case, please turn on Javascript support in your web browser and reload this page.

Logo of nihpaLink to Publisher's site
PMC full text:
Cell Transplant. Author manuscript; available in PMC 2015 Jan 1.
Published in final edited form as:
Cell Transplant. 2014; 23(1): 27–38.
Published online 2013 Feb 4. doi: 10.3727/096368912X662453

Figure 1

An external file that holds a picture, illustration, etc. Object name is nihms468536f1.jpg

Generation of hiPSCs from adult and fetal hepatocytes in entirely feeder-free conditions. Adult or fetal hepatocytes were given lentiviruses delivering the reprogramming factors in their respective culture media overnight (Day 0). The culture medium was changed to mTeSR™1 on Days 1–7. The pictures are representative images of hiPSC lines grown on matrigel in mTeSR™1. The hiPSC lines are morphologically indistinguishable from the H1 hESC line and grow in colonies with well defined borders and high nuclei to cytoplasm ratio. Original magnification of all pictures is X100. (A) Representative colony of fetal hepatocyte-derived hiPSC clone FH1. FH1 was derived using only OCT4, SOX2 and NANOG. (B) Representative colony of adult hepatocyte-derived hiPSC clone AH1. (C) Representative colony of adult hepatocyte-derived hiPSC clone made from cells of a patient diagnosed with CN-1. Clone AH-CN. (D) Representative colony of H1 hESC.

Images in this article

  • Figure 1
  • Figure 2
  • Figure 3
  • Figure 4
  • Figure 5
  • Figure 6
  • Figure 7
Click on the image to see a larger version.