Fig. 2

Hu14.18-IL2 treatment for mice with smaller initial tumor volume leads to increased tumor NK infiltration and NKG2D expression on tumor NK and CD8+ T cells. A/J mice bearing subcutaneous NXS2 tumors were treated with IT-IC, IV-IC, or untreated. Mice were killed 4 days post-treatment initiation and tumors underwent flow cytometric analysis. Initial tumor volumes of all mice are stratified into groups greater or less than the median value of 101.6 mm³. a post-treatment tumor leukocytes (CD45+ cells) are presented as a percentage of total live nucleated cells (TNCs) within the tumor; b macrophages (F4/80+) are presented as a percentage of leukocytes (CD45+) within the tumor; c natural killer cells (NKp46+) are presented as a percent of mouse leukocytes (CD45+); d cytotoxic T cells (CD8+) are presented as a percent of mouse leukocytes (CD45+); e NKG2D expression levels are presented as MFI on NKp46+- and NKG2A+- activated natural killer cells; and f NKG2D expression levels are presented as MFI on CD8+- and NKG2A+- activated cells. Results represent data from 6 independent experiments with an average of 19 mice within each treatment group