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Figure 6.

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Dicer is required for the gene regulatory effects of miR-TAR-3p. (A) Immunoblot (IB) analysis showing Dicer protein down-regulation induced upon Dox treatment (upper panels), in parallel with actin control (lower panels). (B and C) Stable 293T-REx + pcDNA6TR cells conditionally expressing a shRNA directed against Dicer mRNA (shDICER-6 and shDICER-27), or a deleted region in Rluc mRNA (shNeg-13), were induced with 1 µg/ml of Dox for 12 h (+Dox) before transfection with plasmid expressing the (B) TAR RNA/miR-TAR-3p sensor, or (C) shRluc/Rluc sensor combination. Mean ± SEM (n = 4 experiments, in duplicate). *P < 0.05, **P < 0.01 or ***P < 0.005 versus prior induction (−Dox) (Student's t-test).

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