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PMC full text:
Curr Biol. Author manuscript; available in PMC 2009 May 14.
Published in final edited form as:
Curr Biol. 2009 Jan 13; 19(1): 9–19.
doi: 10.1016/j.cub.2008.12.006

Figure 2

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Condensin Subunit Interactions Identified by Immunoprecipitation

(A) Immunoprecipitation (IP) from embryo extracts with antibodies against DPY-27, SMC-4, KLE-2, CAPG-1, and KLP-7 (control) were analyzed by MudPIT mass spectrometry. Numbers indicate the total mass spectra collected in three (KLE-2) or four (all others) IP samples corresponding to each protein indicated on the left.

(B and C) IP reactions from embryo extracts with antibodies against condensin subunits were performed and analyzed on western blots probed with an array of antibodies. Preimmune IP (Pre-im) is shown as control. Selected western blots are shown in (B); summary of all IPs analyzed is shown in (C). Plus sign indicates subunit was detected in the IP; minus sign indicates subunit was not detected in the IP; blank cell indicates interaction was not scored.

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