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Fig. 3.

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ALIXBro1-CHMP4 interfaces. (A) Stereoview of the ALIXBro1-CHMP4A interface. The CHMP4A helix is oriented N to C from top to bottom, ALIX residues within the binding interface are shown explicitly, dashed lines indicate hydrogen bonds or salt bridges, and key hydrophobic CHMP4 residues are underlined. (B) Stereo-view showing an overlay of the bound CHMP4A-C helices. The orientation is the same as in A, and the three key hydrophobic CHMP4 binding residues are shown in sticks. Note that the CHMP4A (purple) and CHMP4C (orange) helices overlay well, whereas the CHMP4B helix (green) is rotated by ≈20°.

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