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Published online 2024 Apr 26. doi: 10.1186/s12885-024-12229-2

Fig. 3

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Identification of shRNA-mediated stable knockdown of Dsg2 and Dsc2 in BC cell lines. (A) The comparison of protein levels from four clones (#5, #6, #7 and#8) of shDsg2 and three clones (#31, #32 and#33) of shDsc2 with their controls (shControl1 and shControl2) in MDA-MB-231 cells and MCF-7 cells by western blot, respectively. (B) The further confirmation of knockdown efficiency of clones #6 and #32 by immunofluorescence. Scale bar represents 100 μm. All experiments were repeated three times; Data are presented as mean ± SD from the three separate experiments. *indicates P < 0.05

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