FIG. 2.

GFP-dASF and GFP-B52 induce disorganization of R and cone cells in eye imaginal discs of transgenic third-instar larvae. (A) Direct fluorescence analysis of the GFP-NLS fusion protein expressed in the larval eye reveals the clusters of developing R cells that emerge in a rectangular array. (B and C) The R-cell organization is slightly altered in the GMR/GFP-dASF#1 larval eye (B) and strongly impaired in GMR/GFP-B52#6 transgenics (C). Magnification, ×20 or ×100 (insets). (D to F) Direct fluorescence analysis and DAPI (4′,6′-diamidino-2-phenylindole) staining of the whole developing eye confirm the differential alterations of R-cell organization in GMR/GFP-dASF#1 (E) and GMR/GFP-B52#6 (F) larval eyes compared to that observed in the control (D). The position of the MF is indicated by arrowheads. Magnification, ×20 or ×100 (insets). (G to I) Anti-Cut immunostaining of cone cells indicates that the number of these differentiated cells is significantly reduced in the developing eyes of GMR/GFP-dASF#1 (H) and GMR/GFP-B52#6 (I) third-instar transgenic larvae compared to that of the control (G). Magnification, ×40. (J to L) Anti-caspase 3 immunostaining of the control larval eye (J) reveals apoptotic cells located on the posterior side of the MF (arrowheads). In GMR/GFP-dASF#1 transgenics (K), numerous apoptotic cells are also disseminated throughout the posterior part of the eye imaginal disc, whereas in GMR/GFP-B52#6 larvae, apoptotic cells concentrate at the most posterior part of the eye (L). Magnification, ×20.