Pain Related Channels Are Differentially Expressed in Neuronal and Non-Neuronal Cells of Glabrous Skin of Fabry Knockout Male Mice
Figure 4
The expression of pain receptors in α-GalA KO males frontal paws.
A) Immunohistochemistry of 12 µm frozen coronal sections of α-GalA KO males (n = 3) showed different expression at the protein level of the specific pain (Nav1.8), heat (TRPV1) and cold (TRPM8, Immunological Science) receptors in comparison to their WT controls (n = 3). B) The western blot analysis of Nav1.8 expression (∼220 kDa) in the whole protein extract from skin of frontal paws confirmed 1.5 fold increased protein expression of Nav1.8 in case of α-GalA KO males (n = 5) when compared to WT males (n = 5), p = 0.0246. The western blot analysis of TRPV1 expression (∼92 kDa) revealed 1.4 fold increased protein expression in case of α-GalA KO males (n = 3) when compared to WT (n = 3), p = 0.0344. The analysis of TRPM8 (Santa Cruz) expression (∼127 kDa) in the whole protein extract from skin of frontal paws showed the almost half expression decrease in protein expression of TRPM8 in case of α-GalA KO (n = 3) males when compared to WT (n = 3), p = 0.0518. Original images of presented blots are part of supplementary material (Figure S4). Scale bar represents 100 µm.