Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach
Figure 2
Purified Brucella melitensis 16M outer membrane proteins expressed as SUMO-fusion proteins in BL-21 E. coli cells.
OmpW, BtuB, Omp22, and cell surface protein expressed in inclusion bodies were purified by centrifugation, solubilized with guanidine HCl, and separated in gel filtration column. Bacterial lysates containing BP26 and FlgK in the soluble fraction were directly purified in an S-300 gel filtration column. Purified protein fractions were diluted with SDS loading buffer, incubated at 95°C for 5 minutes and electrophoresed in a 12.5% SDS-PAGE gel.