Selective Release of MicroRNA Species from Normal and Malignant Mammary Epithelial Cells
Figure 5
Immature miRNAs are Released at miRNA-Species Specific Rates.
A and B, pre-miRNAs of MCF7 cells (c), and miRNAs released from these cells (x) were amplified by rt-PCR and subjected to native PAGE. All the main bands correspond to the expected size of the amplification products of immature miRNAs (arrowheads). Some of the amplification products were confirmed by sequencing (Table S7). C Those miRNAs that yielded single bands in 3 independent experiments were quantified by qRT-PCR. D Presence of upstream RNA sequences, corresponding to pri-miRNA sequences (Pri-s and Pri-l), and RNA corresponding to pre-miRNAs (Pre) were assed by PCR. The identity of the major products was confirmed by sequencing (Table S7). Ntds: nucleotide size of sizing marker.