Selective Release of MicroRNA Species from Normal and Malignant Mammary Epithelial Cells
Figure 4
miR-451 and miR-1246 are Selectively Released from Cells.
A The ratio of released to retained miRNAs standardized to released and retained levels of miR-16 respectively, was quantified from the indicated cell lines using the linker-ligation qRT-PCR approach. B Native PAGE of products at end-point of quantitative RT-PCR of indicated miRNAs using the stem-loop primer approach, and quantified in C. The major PCR-products, indicated by arrows, correspond to the mature miRNA as expected by size (53 ntds for miR-1246, 53 ntds for miR-23a, and 56 ntds for miR-451) and determined by sequencing (Table S5). The bands with a migration of less then 15 ntds are the PCR primers used in the reaction. The stained material in the well is amplification-independent material of the reaction kit. The minor bands larger than 60 ntds represent artifactual products amplified at low template concentration (Figure S5). All miRNAs were quantified from at least 3 cell and media collections, except for miR-23a, which was measured only twice. Error bars indicate standard deviation.