Analysis of a Gene Regulatory Cascade Mediating Circadian Rhythm in Zebrafish
Figure 2
Three types of genes affected by light entrainment.
(A) The circadian expression of fast light-induced genes, slow light-induced genes, and dark-induced genes and representatives of each group (per2, cry2a, and aanat1) on the microarrays are shown. (B) The distinct kinetics of per2, cry2a, aanat1, and clock genes upon light or dark exposure in larval zebrafish fertilized and raised under either DD or LL conditions respectively for 20 hours starting at 5 dpf (5DD to LL and 5LL to DD). The larval zebrafish remained in the constant conditions (5DD to DD and 5LL to LL) were used as controls. The lowest log2-transformed expression level for each gene was normalized to zero. Two independent time-series experiments were conducted with error bars shown in the figure.