Astragaloside IV May Bind to CD25 Gene to Repress HBV Induced Regulatory T Cell Expression
Pages 118 - 122
Abstract
The Chinese Traditional Medicine (CTM) Bushen formula is found to downregulate the expression of Regulatory T cells (Treg-cells) in chronic Hepatitis B (CHB) patients. This paper investigates the effects of Astragaloside IV, one substance the Bushen formula contains, on HBV induced Treg-cell transformation in animal models. Mice will be injected with HBV and grown for 3 months to simulate CHB patients. Astragaloside IV will be tested on dose-dependency by administering different quantities of the substance. There are two major types of possible results for the effects of Astragaloside IV on the expression of Treg-cells: (1) Astragaloside IV does downregulate Treg-cells, (2) Astragaloside IV does not downregulate Treg-cells. Astragalus mongholicus, the source of Astragaloside IV, is one of the many plants often used in CTM. This study will provide us with more details on the mechanisms which CTM treats diseases by analyzing at a molecular level.
References
[1]
Stoop, J. N., van der Molen, R. G., Baan, C. C., van der Laan, L. J., Kuipers, E. J., Kusters, J. G., & Janssen, H. L. 2005. Regulatory T cells contribute to the impaired immune response in patients with chronic hepatitis B virus infection. Hepatology (Baltimore, Md.), 41 (4), 771–778. https://doi.org/10.1002/hep.20649
[2]
Chisari, F. V., Isogawa, M., & Wieland, S. F. 2010. Pathogenesis of hepatitis B virus infection. Pathologie-biologie, 58 (4), 258–266. https://doi.org/10.1016/j.patbio.2009.11.001
[3]
Tang, B. Z., Li, M., & Gao, Y. Q. 2010. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine, 30(8), 823–827.
[4]
Li, M., Sun, X. H., Zhou, Z. H., Yu, Z., Jin, S. G., Zhu, X. J., & Gao, Y. Q. 2013. Beneficial therapeutic effect of Chinese herbal Bushen formula on CHB patients with mildly elevated alanine aminotransferase by down-regulating CD4+CD25+T cells. Journal of ethnopharmacology, 146 (2), 614–622. https://doi.org/10.1016/j.jep.2013.01.038
[5]
Santegoets, S. J., Dijkgraaf, E. M., Battaglia, A., Beckhove, P., Britten, C. M., Gallimore, A., Godkin, A., Gouttefangeas, C., de Gruijl, T. D., Koenen, H. J., Scheffold, A., Shevach, E. M., Staats, J., Taskén, K., Whiteside, T. L., Kroep, J. R., Welters, M. J., & van der Burg, S. H. 2015. Monitoring regulatory T cells in clinical samples: consensus on an essential marker set and gating strategy for regulatory T cell analysis by flow cytometry. Cancer immunology, immunotherapy: CII, 64 (10), 1271–1286. https://doi.org/10.1007/s00262-015-1729-x
[6]
Wang, Y., Liu, L., Ma, Y., Guo, L., Sun, Y., Liu, Q., & Liu, J. 2019. Chemical Discrimination of Astragalus mongholicus and Astragalus membranaceus Based on Metabolomics Using UHPLC-ESI-Q-TOF-MS/MS Approach. Molecules, 24 (22), 4064. https://doi.org/10.3390/molecules24224064
[7]
Zhang, A., Zheng, Y., Que, Z., Zhang, L., Lin, S., Le, V., Liu, J., & Tian, J. 2014. Astragaloside IV inhibits progression of lung cancer by mediating immune function of Tregs and CTLs by interfering with IDO. Journal of cancer research and clinical oncology, 140 (11), 1883–1890. https://doi.org/10.1007/s00432-014-1744-x
[8]
Evaristo, C., Vahldieck, M., Krauthaeuser, S., Jansen, N., Wilske, N., Richter, A., & Dose, C. 2017. Vio® Dyes meet REAfinity™ Antibodies – antibody-fluorochrome conjugates for high-resolution multiparameter flow cytometry analysis [poster abstract]. Miltenyi Biotec GmbH, Bergisch Gladbach, Germany. https://www.miltenyibiotec.com/_Resources/Persistent/bf0b810a051b7bd6c9a019b1502ebcc1c125d53f/Human%20Treg%20antibody%20panel.pdf
[9]
Srivastava, S., Somasagara, R. R., Hegde, M., Nishana, M., Tadi, S. K., Srivastava, M., Choudhary, B., & Raghavan, S. C. 2016. Quercetin, a Natural Flavonoid Interacts with DNA, Arrests Cell Cycle and Causes Tumor Regression by Activating Mitochondrial Pathway of Apoptosis. Scientific Reports, 6(1). https://doi.org/10.1038/srep24049
[10]
Wang, L., Chi, Y. F., Yuan, Z. T., Zhou, W. C., Yin, P. H., Zhang, X. M., Peng, W., & Cai, H. 2014. Astragaloside IV inhibits renal tubulointerstitial fibrosis by blocking TGF-β/Smad signaling pathway in vivo and in vitro. Experimental biology and medicine (Maywood, N.J.), 239 (10), 1310–1324. https://doi.org/10.1177/1535370214532597
[11]
Yin, F., Zhou, H., Fang, Y., Li, C., He, Y., Yu, L., Wan, H., & Yang, J. 2020. Astragaloside IV alleviates ischemia reperfusion-induced apoptosis by inhibiting the activation of key factors in death receptor pathway and mitochondrial pathway. Journal of Ethnopharmacology, 248, 112319. https://doi.org/10.1016/j.jep.2019.112319
[12]
Mercer, F., & Unutmaz, D. 2009. The biology of FoxP3: a key player in immune suppression during infections, autoimmune diseases and cancer. Advances in experimental medicine and biology, 665, 47–59. https://doi.org/10.1007/978-1-4419-1599-3_4
Index Terms
- Astragaloside IV May Bind to CD25 Gene to Repress HBV Induced Regulatory T Cell Expression
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Published In
October 2022
594 pages
ISBN:9781450398442
DOI:10.1145/3570773
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Published: 09 December 2022
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ISAIMS 2022
ISAIMS 2022: 2022 3rd International Symposium on Artificial Intelligence for Medicine Sciences
October 13 - 15, 2022
Amsterdam, Netherlands
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