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Activity-dependent neuronal cell migration induced by electrical stimulation

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Abstract

Recently, we found that electrical stimulation can induce neuronal migration in neural networks cultured for more than 3 weeks on microelectrode arrays. Immunocytochemistry data showed that the aggregation of neurons was related to the emergence of astrocytes in culture. In this study, when neurons were cocultured with astrocytes, electrical stimulation could induce the migration of neuronal cell bodies after only 1 week in culture, while the same stimulation paradigm caused neural necrosis in neuron-only cultures. In addition, the stimulation-induced migration was inhibited by blocking action potentials in neural networks using the voltage-gated sodium channel blocker, tetrodotoxin. Immunocytochemistry was performed to monitor precisely the neuronal migration and count the number of neurons. These results indicate that neuronal migration of cell bodies is dependent on neuronal activity evoked by electrical stimulation and can be enhanced by coculturing with astrocytes. We believe this method can be employed as a means for modifying neural networks and improving the interface between electrodes and neurons.

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Acknowledgments

This work was supported by the Korea Science and Engineering Foundation (KOSEF) through the Nano Bioelectronics and Systems Research Center [Grant No. R11-2000-075-00002-0 for domestic research and R11- 330 2000-075-01001-0 for international collaboration (June)].

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  1. Sang Beom Jun

    Present address: Laboratory for Integrative Neuroscience, NIAAA/NIH, Bethesda, USA

Authors and Affiliations

  1. Interdisciplinary Program in Brain Science, Seoul National University, Seoul, South Korea

    Se Hoon Jeong & Sung June Kim

  2. School of Electrical Engineering, Seoul National University, Seoul, South Korea

    Sang Beom Jun, Jong Keun Song & Sung June Kim

  3. Nano Bioelectronics and Systems Research Center, Seoul, South Korea

    Se Hoon Jeong, Sang Beom Jun, Jong Keun Song & Sung June Kim

Authors
  1. Se Hoon Jeong
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  2. Sang Beom Jun
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  3. Jong Keun Song
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Correspondence to Se Hoon Jeong.

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Jeong, S.H., Jun, S.B., Song, J.K. et al. Activity-dependent neuronal cell migration induced by electrical stimulation. Med Biol Eng Comput 47, 93–99 (2009). https://doi.org/10.1007/s11517-008-0426-8

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