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Free radical and superoxide reactivity detection in semen quality assessment: past, present, and future

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Abstract

Oxidative stress is a well-established cause of male infertility, with reactive oxygen species (ROS) impairing sperm production, motility, membrane, and DNA integrity. Currently, most clinics do not test infertile patients for the imbalance between ROS generation and the ability of the antioxidants to scavenge them, although there is a clear need for andrology laboratories to be able to identify and/or quantify seminal oxidative stress. As such there is a clinical urgency for an inexpensive and easy-to-perform assay able to identify oxidative stress in semen. The aim of this review is to provide information on the currently available methods to assess and quantify ROS and particularly superoxide in male reproductive cells, tissues, and fluids which may have a significant clinical utility in identifying men with impaired fertility associated with oxidative stress. Through a deeper understanding of oxidative stress and its assessment options, clinical andrology labs may better assist patients to achieve increased rates of fertility and pregnancy.

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Authors and Affiliations

  1. Department of Biology, Universidad Autonoma de Madrid, Edificio de Biología, Darwin 2, 28049, Madrid, Spain

    Jaime Gosalvez

  2. Department of Animal Physiology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic

    Eva Tvrda

  3. American Center for Reproductive Medicine, Cleveland Clinic, Mail Code X-11, 10681 Carnegie Avenue, Cleveland, OH, 44195, USA

    Ashok Agarwal

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  1. Jaime Gosalvez
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Correspondence to Eva Tvrda.

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This study was supported by the Spanish Ministry of Economy and Competitiveness, MINECO (BFU-2013-44,290-R) and by the Slovak Research and Development Agency Grant no APVV-15-0544.

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Gosalvez, J., Tvrda, E. & Agarwal, A. Free radical and superoxide reactivity detection in semen quality assessment: past, present, and future. J Assist Reprod Genet 34, 697–707 (2017). https://doi.org/10.1007/s10815-017-0912-8

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