In this paper, we present sub-1-V CMOS-based electrophoresis method for small-form-factor biomolecule manipulation that is contained in a microchip. This is the first time this type of device has been presented in the literature. By combining CMOS technology with electroless gold plating, the electrode pitch can be reduced and the required input voltage can be decreased to less than 1 V. We fabricated the CMOS electrophoresis chip in a cost-competitive 0.6 µm standard CMOS process. A sample/hold circuit in each cell is used to generate a constant output from an analog input. After forming gold electrodes using an electroless gold plating technique, we were able to manipulate red food coloring with a 0-0.7 V input voltage range. The results shows that the proposed CMOS chip is effective for electrophoresis-based manipulation.