Anti-Plx [AZ44]
Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151301 |
| Applications | ELISA WB |
| Antigen/Gene or Protein Targets | Polo-like-kinase protein (Plk) |
| Synonyms | Polo Like Kinase 1; Serine/Threonine-Protein Kinase 13; EC 2.7.11.21; STPK13; PLK-1; Cell Cycle Regulated Protein Kinase; Polo Like Kinase; EC 2.7.11 |
| Reactivity | Xenopus laevis |
| Relevance | Polo-like kinase (Plx) is a mitotic regulator conserved from yeasts to humans. Many key cell cycle regulators such as p53, cdc25, cyclin B and the Anaphase Promoting Complex are directly targeted by Plx.The Anaphase promoting complex is responsible for the destruction of cyclins and other proteins involved with mitosis. |
| Host | Mouse |
| Immunogen | Baculo expressed Polo-like-kinase from Xenopus laevis |
| Positive Control | Xenopus egg extract |
| Subclass | IgG1 kappa |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes | Plx is activated by phosphorylation. AZ44 only detects the mitotic form of Plx (Xenopus laevis) which may be due to the recognition of a phosphorylated epitope. Recommended concentration: 1ug/ml |
| Research Area | Cell Type or Organelle Marker |
References: 2 entries
Esmaili et al. 2010. Cell Cycle. 9(19):3913-20. PMID: 20935510.
WB
Wu et al. 2009. Nat Cell Biol. 11(5):644-51. PMID: 19396163.
WB
Add a reference
References: 2 entries
Esmaili et al. 2010. Cell Cycle. 9(19):3913-20. PMID: 20935510.
WB
Wu et al. 2009. Nat Cell Biol. 11(5):644-51. PMID: 19396163.
WB
Add a reference