Anti-Cdk1 [17 (A17)]
Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (12)
- Inventor Info
Info
| Catalogue Number | 151037 |
| Applications | ELISA IHC IP WB |
| Antigen/Gene or Protein Targets | Cyclin dependent kinase 1 (Cdk1, cdc2) |
| Synonyms | Cyclin-Dependent Kinase 1; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; P34 Protein Kinase; P34CDC2; CDC28A; CDC2; Cell Cycle Controller CDC2; CDKN1 |
| Reactivity | Chicken, Human, Mouse, Rat and Xenopus laevis |
| Relevance | Cdk1 (Cdc2) is a serine/threonine kinase which forms complexes with cyclins A and B which then phosphorylate a variety of target substrates to enable cell cycle progression. Therefore, Cdk1 is a marker for proliferating tumour cells. |
| Host | Mouse |
| Immunogen | A carboxy-terminal fragment starting at methionine 85 of p34 cdc2 (Xenopus laevis). |
| Subclass | IgG2a |
| Molecular Weight (kDa) | 34 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS. |
| Strain | Balb/c |
| Notes |
Recommended for formulin fixed immunohistochemistry samples. Tested Applications Dilution Radioimmune Assays (RIA) Assay dependent Immunocytochemistry (ICC) 5 µg/ml Immunomicroscopy (IM) Assay-dependent ELISA (ELISA) Assay dependent Immunoprecipitation (IP) 2.5 µg/I Western Blot (WB) 1µg/ml Immunofluorescence (IF) Assay dependent Immunohistochemistry (Paraffin, Frozen) (IHC (P, F)) 5 µg/ml |
| Research Area | Cancer, Cell Cycle, Cell Type or Organelle Marker, Neurobiology |
References: 12 entries
Zhou et al. 2015. Platelets. :1-11. PMID: 26325015.
Platelets promote cartilage repair and chondrocyte proliferation via ADP in a rodent model of osteoarthritis.
Europe PMC ID: 26325015
Coulonval et al. 2011. Mol Biol Cell. 22(21):3971-85. PMID: 21900495.
Coupling of T161 and T14 phosphorylations protects cyclin B-CDK1 from premature activation.
Europe PMC ID: 21900495
Gannon et al. 1998. Genes Cells. 3(1):17-27. PMID: 9581979.
A measure of the mitotic index: studies of the abundance and half-life of p34cdc2 in cultured cells and normal and neoplastic tissues.
Europe PMC ID: 9581979
Goodger et al. 1996. J Pathol. 178(4):422-8. PMID: 8691321.
The localization of p34cdc2 in the cells of normal, hyperplastic, and malignant epithelial and lymphoid tissues of the oral cavity.
Europe PMC ID: 8691321
Doussis-Anagnostopoulou et al. 1994. Histopathology. 24(4):335-40. PMID: 8045523.
Distribution of the cdc2 gene product in normal tissues: an immunocytochemical study using four new monoclonal antibodies.
Europe PMC ID: 8045523
Kobayashi et al. 1992. Mol Biol Cell. 3(11):1279-94. PMID: 1333843.
Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.
Europe PMC ID: 1333843
Add a reference
References: 12 entries
Zhou et al. 2015. Platelets. :1-11. PMID: 26325015.
Platelets promote cartilage repair and chondrocyte proliferation via ADP in a rodent model of osteoarthritis.
Coulonval et al. 2011. Mol Biol Cell. 22(21):3971-85. PMID: 21900495.
Coupling of T161 and T14 phosphorylations protects cyclin B-CDK1 from premature activation.
Gannon et al. 1998. Genes Cells. 3(1):17-27. PMID: 9581979.
A measure of the mitotic index: studies of the abundance and half-life of p34cdc2 in cultured cells and normal and neoplastic tissues.
Goodger et al. 1996. J Pathol. 178(4):422-8. PMID: 8691321.
The localization of p34cdc2 in the cells of normal, hyperplastic, and malignant epithelial and lymphoid tissues of the oral cavity.
Doussis-Anagnostopoulou et al. 1994. Histopathology. 24(4):335-40. PMID: 8045523.
Distribution of the cdc2 gene product in normal tissues: an immunocytochemical study using four new monoclonal antibodies.
Kobayashi et al. 1992. Mol Biol Cell. 3(11):1279-94. PMID: 1333843.
Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.
Add a reference