1588022427pipettes, Care and Uses
1588022427pipettes, Care and Uses
1588022427pipettes, Care and Uses
• Hold pipette in solution, don’t touch the bottom, squeeze bulb and
attach to top of the pipette. Use the forefinger to control volume
aspiration by placing on top of pipette.
• Subtract the needed volume from the initial volume and find the
dispensing volume in order to get the desired amount.
Graduated/Serological Pipette
Pipettes
• Single-Channel Pipette: Also known as automatic pipette, it is a
non-disposable instrument usually of an air-displacement design
which produces an accurate measurement results with the use of
one disposable tip. The two common techniques are:
• The tip of the filled pipette is held against the side of the receptacle
to dispense the liquid content
• The plunger is slowly pressed through the first stop to the final
Single-Channel Pipette
• Reverse Technique is most effective when working with
viscous solutions or solutions prone to bubbles, it minimizes
interference from air bubbles.
• Press plunger all the way down to the third stop position,
slightly submerge into liquid and slowly release the plunger
back to the top to aspirate the liquid into the tip.
• Place the pipette tip against the receptacle wall and press
plunger to the first stop to empty the content and remove
the tip from the receptacle.
• Move the end of the tip away from the liquid and release the
plunger to the rest position.
Multichannel Pipette
Pipettes
• Repeat Pipette Dispenser: Allows the setting and
dispensing of a specific volume into multiple receptacles
without having to aspirate in between dispenses.