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    Oil Reservoir Simulation Bioreactor - Methane Production Rate - Hydrocarbon Degradation Metabolites - Characterization of Microbial Community Shift

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    1) The document describes a study using an anaerobic bioreactor at 55°C to degrade oil from oil-bearing sand over 520 days. 2) Analysis of the microbial community showed a shift from bacteria to archaea involved in syntrophic acetate oxidation and hydrogenotrophic methanogenesis. 3) The study demonstrates the feasibility of using crude oil waste to enhance energy recovery through a microbial enhanced process.

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    Oil Reservoir Simulation Bioreactor - Methane Production Rate - Hydrocarbon Degradation Metabolites - Characterization of Microbial Community Shift

    Uploaded by

    geethai
    16 views10 pages
    1) The document describes a study using an anaerobic bioreactor at 55°C to degrade oil from oil-bearing sand over 520 days. 2) Analysis of the microbial community showed a shift from bacteria to archaea involved in syntrophic acetate oxidation and hydrogenotrophic methanogenesis. 3) The study demonstrates the feasibility of using crude oil waste to enhance energy recovery through a microbial enhanced process.

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    hydrocarbon degradation

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    1) The document describes a study using an anaerobic bioreactor at 55°C to degrade oil from oil-bearing sand over 520 days. 2) Analysis of the microbial community showed a shift from bacteria to archaea involved in syntrophic acetate oxidation and hydrogenotrophic methanogenesis. 3) The study demonstrates the feasibility of using crude oil waste to enhance energy recovery through a microbial enhanced process.

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    Download as pptx, pdf, or txt
    16 views10 pages

    Oil Reservoir Simulation Bioreactor - Methane Production Rate - Hydrocarbon Degradation Metabolites - Characterization of Microbial Community Shift

    Uploaded by

    geethai
    1) The document describes a study using an anaerobic bioreactor at 55°C to degrade oil from oil-bearing sand over 520 days. 2) Analysis of the microbial community showed a shift from bacteria to archaea involved in syntrophic acetate oxidation and hydrogenotrophic methanogenesis. 3) The study demonstrates the feasibility of using crude oil waste to enhance energy recovery through a microbial enhanced process.

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    © All Rights Reserved
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    of 10

    International Biodeterioration & Biodegradation (2019)

    • Oil reservoir simulation bioreactor

    • Methane production rate

    • Hydrocarbon degradation metabolites

    • Characterization of Microbial community shift


    Bioreactor conditions:
    • Anaerobic
    • Ambient atmospheric pressure at 55°C
    • Reactor volume: 600mL
    • Duration: 520 days
    • Substrate : Oil-bearing sand (0.03g of oil/g of sand)
    • Oil-bearing sand: 500g of sterile sand + 15g of crude oil
    • Aging at 105° for two weeks
    Modified Gompertz Model
    A – Biogas yield potential (µmol)
    µm - Maximum biogas production rate
    (µmol/d)
    λ- Lag phase (d)
    Y – cumulative biogass production (µmol)
    Bacterial Composition

    PW – Production water
    BR341 – bioreactor sampled
    on day 341
    BR520 – Bioreactor sampled
    on day 520
    Archael composition

    PW – Production water
    BR341 – bioreactor sampled on day
    341
    BR520 – Bioreactor sampled on day
    520
    Reactions involved in acetate and hydrogen metabolism

    Hattori, S. (2008). Syntrophic Acetate-Oxidizing Microbes in Methanogenic Environments. Microbes and


    Environments.
    Average CH₄ production rate
    Phase I : 4.9mmol CH₄/day/cubic metre of pore volume
    Phase II : 20.8 mmol CH₄/day/cubic metre of pore volume
    Intermediate metabolites:
    BR341 – octadecanoic acid
    BR520 - Nonanoic acid and dodecanoic acid
    VFA : Formate, acetate, propionate, isobutyrate and butyrate
    Overview of laboratory studies on methanogenic Hydrocarbon degradtion
    Substrate Source of the Incubation Porous media Incubator CH₄ References
    inoculum Temperature addition production
    rate
    Residual oil Gas- Room T Crushed core Serum bottles 0.15- Gieg
    condensate- material (60mL) 0.40µmol etal.,2008
    contaminated CH₄/day/g
    sediments core
    Crude oil Petroleum 55°C Glass beads Serum bottles NA (70% of Gieg et
    waters from (60mL) theoretical al.,2010
    hot oilfields yield)
    Crude oil Production 55°C No Stainless-steel NA Mayumi et
    water from a cylinder al., 2011
    high T bottles
    Crude oil Production Room T Crushed Sandstone- 5.8µmol Berdugo-
    waters from a Berea packed CH₄/day/g oil Clavijo and
    low- sandstone coloums Gieg, 2014
    Temperature core (30ml)
    Crude Oil Formation 30°C no Serum bottles 76µmol Cai et al.,
    water (120mL) CH₄/day/g oil 2015
    Inference:
    • Thermophilic hydrocarbon degradation takes place.
    • Syntrophic acetate oxidation and hydrogenotrophic methanogenesis
    occurs .
    • Hence Microbial enhance energy recovery process is feasible using
    crude oil waste.
    Microbial Selection
    Thermophiles:
    • Higher solubility of hydrophobic pollutants
    • Decreases viscosity
    • Diffusion of pollutants is considerably faster
    MMethanogenic
    Biosurfactant producing • Aliphatic hydrocarbons are liquid at higher T
    microbial consortium
    strains
    Mass transfer limitations
    Community
    Adaptation

    Bioslurry reactor
    Methanogenesis Bioenergy
    Studies

    • Pathway Indentification
    • Thermodynamic BioSolids
    properties and kinetics
    • Process optimization

    Overall Picture

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