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    DETERMINATION OF BIOACTIVE COMPOUNDS FROM Piper Nigrum. L BY USING HPLC ANALYSIS

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    Baru Chandrasekhar Rao
    Black pepper is important for its medical value (Parganiha et al., 2011). Medicinally black pepper can be used for digestive disorders like large intestine toxins, different gastric problems, diarrhea, and indigestion and also can be used against respiratory disorders including cold, fever and asthma. Black pepper is used to treat asthma, chronic indigestion, colon toxins, obesity, sinus, congestion, fever intermittent fever, cold extremities, colic, gastric ailments and diarrhea. In this Present Study the bioactive compounds from the plant Piper nigrum L. were determined by using HPLC Analysis. Key words: Black pepper, Piper nigrum L., HPLC

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    DETERMINATION OF BIOACTIVE COMPOUNDS FROM Piper Nigrum. L BY USING HPLC ANALYSIS

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    Baru Chandrasekhar Rao
    34 views3 pages
    Black pepper is important for its medical value (Parganiha et al., 2011). Medicinally black pepper can be used for digestive disorders like large intestine toxins, different gastric problems, diarrhea, and indigestion and also can be used against respiratory disorders including cold, fever and asthma. Black pepper is used to treat asthma, chronic indigestion, colon toxins, obesity, sinus, congestion, fever intermittent fever, cold extremities, colic, gastric ailments and diarrhea. In this Present Study the bioactive compounds from the plant Piper nigrum L. were determined by using HPLC Analysis. Key words: Black pepper, Piper nigrum L., HPLC

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    DETERMINATION OF BIOACTIVE COMPOUNDS FROM Piper nigrum. L BY USING HPLC ANALYSIS

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    Black pepper is important for its medical value (Parganiha et al., 2011). Medicinally black pepper can be used for digestive disorders like large intestine toxins, different gastric problems, diarrhea, and indigestion and also can be used against respiratory disorders including cold, fever and asthma. Black pepper is used to treat asthma, chronic indigestion, colon toxins, obesity, sinus, congestion, fever intermittent fever, cold extremities, colic, gastric ailments and diarrhea. In this Present Study the bioactive compounds from the plant Piper nigrum L. were determined by using HPLC Analysis. Key words: Black pepper, Piper nigrum L., HPLC

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    34 views3 pages

    DETERMINATION OF BIOACTIVE COMPOUNDS FROM Piper Nigrum. L BY USING HPLC ANALYSIS

    Uploaded by

    Baru Chandrasekhar Rao
    Black pepper is important for its medical value (Parganiha et al., 2011). Medicinally black pepper can be used for digestive disorders like large intestine toxins, different gastric problems, diarrhea, and indigestion and also can be used against respiratory disorders including cold, fever and asthma. Black pepper is used to treat asthma, chronic indigestion, colon toxins, obesity, sinus, congestion, fever intermittent fever, cold extremities, colic, gastric ailments and diarrhea. In this Present Study the bioactive compounds from the plant Piper nigrum L. were determined by using HPLC Analysis. Key words: Black pepper, Piper nigrum L., HPLC

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    of 3

    IAJPS 2018, 05 (04), 2919-2921 N.K.

    Parameswaran et al ISSN 2349-7750

    CODEN [USA]: IAJPBB ISSN: 2349-7750

    INDO AMERICAN JOURNAL OF


    PHARMACEUTICAL SCIENCES
    http://doi.org/10.5281/zenodo.1229564

    Available online at: http://www.iajps.com Research Article

    DETERMINATION OF BIOACTIVE COMPOUNDS FROM Piper


    nigrum. L BY USING HPLC ANALYSIS
    S. Manjusha1*, N.K.Parameswaran2, R.Senthil Malar3
    1
    Department of Botany and Research Centre, Scott Christian College (Autonomous,) Nagercoil-
    629003, Kanyakumari district, Tamil Nadu, India.
    2
    Department of Biotechnology, Manonmaniam sundaranar University, Tirunelveli
    3
    Department of Zoology, Sivanthi Adithanar College, Nagercoil
    Abstract:
    Black pepper is important for its medical value (Parganiha et al., 2011). Medicinally black pepper can be used for
    digestive disorders like large intestine toxins, different gastric problems, diarrhea, and indigestion and also can be
    used against respiratory disorders including cold, fever and asthma. Black pepper is used to treat asthma, chronic
    indigestion, colon toxins, obesity, sinus, congestion, fever intermittent fever, cold extremities, colic, gastric ailments
    and diarrhea. In this Present Study the bioactive compounds from the plant Piper nigrum L. were determined by
    using HPLC Analysis.
    Key words: Black pepper, Piper nigrum L., HPLC
    Corresponding author:
    N.K.Parameswaran, QR code
    Department of Biotechnology,
    Manonmaniam sundaranar University,
    Tirunelveli.
    Email- nkparamesh@gmail.com
    Mob: 9489154966
    Please cite this article in press N.K.Parameswaran et al., Determination of Bioactive Compounds from piper
    nigrum. L by using HPLC analysis, Indo Am. J. P. Sci, 2018; 05(04).

    www.iajps.com Page 2919


    IAJPS 2018, 05 (04), 2919-2921 N.K.Parameswaran et al ISSN 2349-7750

    INTRODUCTION: High Performance Liquid chromatography


    Piper nigrum L. finds extensive use in Ayurvedic (HPLC) analysis
    system of medicine. A number of piperidine and The leaf and seed samples were further analysed in
    pyrrolidine alkamides are known to occur in Piper high performance liquid chromatography (WATER,
    nigrum L. the most important being piperine, known Germany) with the software BREEZE (ver.2.1). 10 µl
    to possess a variety of biological properties like CNS of the sample extract was filled in capillary column
    stimulant, analgesic, antipyretic and antifeedant of the instrument, run time was 10 minutes. Retention
    activities. Pepper is a natural spice. Piper nigrum L. time (min), area (V* sec), % area, height (V* sec), %
    finds an extensive application in antibacterial height, starting time (min) and end time (min) of the
    preparations. Fractionation of the petroleum ether peaks were noted.
    extract of the berries of Piper nigrum L. afforded 2E,
    4E, 8Z-N-isobutyleicosatrienamide, pellitorine, RESULTS:
    trachyone, pergumidiene and isopiperolein B [1]. High Performance Liquid chromatography
    Pergumidiene and trachyone have also been isolated (HPLC) Analysis
    from Piper nigrum L. All the isolated compounds In HPLC analysis, the ethanol extracts of the samples
    have shown activity against Bacillus subtilis, Bacillus were used. In this present study, the Piper nigrum L.
    sphaericus and Staphylococcus aureus amongst leaf showed five peaks between the retention time of
    Gram +ve bacteria, and Klebsiella aerogenes and 1 to 10 minutes were as 1.551, 1.826, 2.126, 2.324
    Chromobacterium violaceum among Gram –ve and 4.094. Among these, three peaks were found as
    bacterial strains [2]. high and larger volume, 1st one RT (min) = 1.826,
    area (V* sec) = 1048235, % area = 59.81, height (V*
    MATERIALS AND METHODS: sec) = 47185, % height = 46.55, starting time (min) =
    Selection of Plant material 1.283 and end time (min) = 2.050; 2nd one RT (min)
    In this present study, the plant Piper betle L. leaves = 2.126, area (V* sec) = 272315, % area = 15.54,
    and seeds were collected in Pechiparai Hill Region height (V* sec) = 28343, % height = 27.96, starting
    Kanyakumari District, Tamilnadu. An adult, fresh time (min) = 2.050 and end time (min) = 2.250 and
    leaves were picked out from the plant and also the 3rdone RT (min) = 2.342, area (V* sec) = 384438, %
    matured seed were collected from the plants and area = 21.94, height (V* sec) = 22759, % height =
    transported to the laboratory for work. 22.45, starting time (min) = 2.250 and end time (min)
    = 2.983 (Plate 22).
    The collected leaves were subjected to surface
    cleaning by rinsing the samples with sterile water, in Piper nigrum L. fruit showed five peaks between the
    order to remove dust particles present on the plant retention time of 1 to 10 minutes were as 1.213,
    materials. The samples such as leaf and seeds were 1.551, 1.685, 7.169 and 7.395. Among these, two
    allowed to shade dry to remove moisture content. The peaks were found as high and larger volume, 1st one
    dried samples were used for further studies. RT (min) = 7.169 area (V* sec) = 147457, % area =
    40.52, height (V* sec) = 4776, % height = 41.08,
    Preparation of Plant Extracts starting time (min) = 6.167 and end time (min) =
    The leaves were cut into small pieces and seeds were 7.200; and 2nd one RT (min) = 7.395, area (V* sec) =
    made powdered using electric mixer grinder. All the 205221, % area = 56.39, height (V* sec) = 5228, %
    samples were subjected to soxhlet extraction using height = 44.97, starting time (min) = 7.200 and end
    five solvents such as Acetone, Chloroform, Dimethyl time (min) = 8.300 (Plate 23). In HPLC analysis,
    sulfoxide, Ethanol and Distilled water. Each 5grams Piper nigrum L. leaf showed five peaks and the
    of plant material was filled separately in the thimble major peaks were RT (min) = 1.826, area (V* sec) =
    and extracted successively with 60ml of solvents 1048235; RT (min) = 2.126, area (V* sec) = 272315;
    using a soxhlet extractor for three hours. After and RT (min) = 2.342, area (V* sec) = 384438. Piper
    solvent evaporation, each of these solvent extract was nigrum L. seed showed five peaks and the major
    weighed and preserved in room temperature until peaks were RT (min) = 7.169 area (V* sec) =
    further use. 147457; and RT (min) = 7.395, area (V* sec) =
    205221.

    www.iajps.com Page 2920


    IAJPS 2018, 05 (04), 2919-2921 N.K.Parameswaran et al ISSN 2349-7750

    HPLC Chromatogram of Leaf Extract of Piper nigrum L. PLC Chromatogram of Fruit Extract of Piper
    nigrum L.

    CONCLUSION: anti- asthmatic activity of fruit extract of Piper


    Almost all the members of Piperaceae are used in the nigrum L. (Piperaceae). International Journal of
    traditional medicinal system. Species like Piper Herbal Drug Research, 1: 15-18.
    nigrum L., Piper betle L. and Piper longum L. ranks 4. Sujatha, R., Luckin, C.B. and Nazeem, P.A.
    first in the siddha medicinal use. For most of the 2003. Histology of organogenesis from callus
    siddha medicinal preparation any one of the cultures of black pepper (Piper nigrum L.)
    Piperaceae member being an incredient. In modern Journal of Tropical Agricuture, 41: 16-19.
    days, medicinal plants are becoming probable sources 5. Ravindran, P.N. 2000. Black Pepper: Piper
    of important drugs and pharmaceutical industries. nigrum. Series: Medicinal and Aromatic Plants –
    Nowadays, they have come to consider traditional Industrial Profiles. Centre for Medicinal Plants
    medicine as a source of bioactive agents which can Research, India. Publisher Availability: In Stock
    be used in the preparation of synthetic medicine. CRC Press, Kerala. pp. 1-526.
    6. Ao, P., Hu, S. and Zhao, A. 1998. Essential oil
    REFERENCES: analysis and trace element study of the roots of
    1. Das, B., Kashinatham, A. and Srinivas, K.V. Piper nigrum L. Journal of Zhongguo ZhongYao
    1998. Alkamides and other constituents of Piper ZaZhi, 23(1): 42-63.
    longum L. Planta Medica, 62: 582-583. 7. Ganesh, P., Kumar, R.S. and Saranraj, P. 2014.
    2. Jayaweera, D.M.A. 1982. Medicinal Plants Used Phytochemical analysis and antibacterial activity
    in Ceylon. National Science Council of of Pepper (Piper nigrum L.) against some human
    SriLanka, Colombo, 5: 201. pathogens. Central European Journal of
    3. Parganiha, R., Verma, S., Chandrakar, S., Pal, S., Experimental Biology, 3(2): 36-41.
    Sawarkar, H.A. and Kashyap, P. 2011. In vitro

    www.iajps.com Page 2921

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