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    CV Sharif Hala Feb - 2017

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    Sharif Hala
    Sharif Matouq Ahmed Hala has over 15 years of experience in biomedical research. He is currently a PhD candidate at KAUST researching patient samples using NGS methods. His background includes founding two startups, obtaining a PhD from KAUST, and research experience in stem cell biology, molecular biology, and medical genetics from universities in the UK and US. He has published papers and presentations in his areas of expertise, which include stem cell differentiation, genome sequencing, and single nucleotide polymorphism analysis.

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    CV Sharif Hala Feb - 2017

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    Sharif Hala
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    Sharif Matouq Ahmed Hala has over 15 years of experience in biomedical research. He is currently a PhD candidate at KAUST researching patient samples using NGS methods. His background includes founding two startups, obtaining a PhD from KAUST, and research experience in stem cell biology, molecular biology, and medical genetics from universities in the UK and US. He has published papers and presentations in his areas of expertise, which include stem cell differentiation, genome sequencing, and single nucleotide polymorphism analysis.

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    CV Sharif Hala Feb- - 2017

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    Sharif Matouq Ahmed Hala has over 15 years of experience in biomedical research. He is currently a PhD candidate at KAUST researching patient samples using NGS methods. His background includes founding two startups, obtaining a PhD from KAUST, and research experience in stem cell biology, molecular biology, and medical genetics from universities in the UK and US. He has published papers and presentations in his areas of expertise, which include stem cell differentiation, genome sequencing, and single nucleotide polymorphism analysis.

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    104 views6 pages

    CV Sharif Hala Feb - 2017

    Uploaded by

    Sharif Hala
    Sharif Matouq Ahmed Hala has over 15 years of experience in biomedical research. He is currently a PhD candidate at KAUST researching patient samples using NGS methods. His background includes founding two startups, obtaining a PhD from KAUST, and research experience in stem cell biology, molecular biology, and medical genetics from universities in the UK and US. He has published papers and presentations in his areas of expertise, which include stem cell differentiation, genome sequencing, and single nucleotide polymorphism analysis.

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    Curriculum Vitae

    Sharif Matouq Ahmed Hala


    Tel: 02-6610061
    Mobile: 00966-501057777
    E-mail: Sharif.Hala@gmail.com

    Career Objective
    - To work in a challenging professional research position, which in-turn
    will offer interesting prospects of growth, progress and exciting
    advancement

    - To obtain the highest level –possible- of learning and apply it in future


    biotechnology and medical research applications

    - To apply research outcomes through finding a start-up biotechnology


    company

    Profile
    - To utilize my interpersonal and distinguished communication skills,
    determination, international collaborative networks and lab skills in
    joining a research team to contribute to their research complex genetic
    traits in relations to diseases.

    - To use my background in molecular biology, medical genetics, stem


    cell and musculoskeletal science to tinker with old products to
    develop new methods and protocols.

    - To excel in the fields of biomedical research and to significantly


    contribute towards the growth of a dynamic organization that offers
    challenging opportunities for proficient accomplishments.

    Education and Work Experience


     Co-founder of Meteriod “Innovative communication techonology”.
    Taqadam program start-up winner.

     Co-founder of Algorizm “Genetic Sequencing Company”.


    www.algorizmsa.com

     PhD Candidate – King Abdullah University of Science and Technology


    (KAUST) 2015-present
    Researching patient samples at KAUST using NGS methods at the pathogen genomics laboratory of
    Prof. Arnab Pain.
     National Guard Health Affairs (NGHA) – King Abdullah Medical Research
    Centre (KAIMRC) 2013 – Present
    Working as Scientific Research Assistant. Undergoing multiple projects and in charge of the
    confocal imaging systems.

     University of Oxford, UK – Stem Cell – Research projects Jan2011 –


    May2013
    Research in Stem Cell Epigenetics & Musculoskeletal Sciences

     University of Aberdeen, UK - MSc Aug2008 - Jun2010


    Masters degree in Medical Molecular Genetics: a one-year degree, which
    includes a six months research project.

     University of Aberdeen, UK - BSc May2003 - Jun2008


    Bachelor degree in Molecular Biology with Honors. : A four-year degree that
    includes a one-year honor/intensive of hands-on research and work
    experiences.

     University of Wisconsin Superior, Wisconsin, USA Oct2000 - Jul2002


    - TOFEL intensive course at SESLI office for one year.

    - Introduction to Biology and Environmental science: a one-year course was


    directed toward understanding environmental issues and organismal
    behavioral due to cultural changes.

    Research Experience
    The combined laboratory and research
    projects/experience yield a total of nine years.

    Research Projects
     Next Generation Sequencing (NGS) based metagenomics and
    targeted genomic analysis of microbial communities present in
    various patient-derived aspirations, bodily fluids, or bacterial/fungal
    isolates from patients at the MNGHA.
     The use of femtosecond near infrared laser microscopy for targeted
    transfection of different somatic mammalian cells as well as adipose
    derived stem cells with non-viral vectors containing transcription
    factors (Oct4, Sox2 and Klf4) individually into induced pluripotent
    stem cell generation.
     Generation of the induced pluripotent stem cell using non-viral
    methods (Originally generated by S.Yamanka the NOBEL prize winner
    2012 on the generation of Human iPSCs) by electroporation of three
    plasmids containing embryonic reprogramming factors (Oct4, Sox2
    and Klf4) and detection plasmid as green fluorescent protein. This
    project involved the use of LSM710 confocal/multiphoton microscope
    and Becton Dickson Pathway.

    2
     Differentiation of human induced pluripotent stem cells into
    mesodermal linage into chondrocyte to form cartilage for future
    clinical trials. The project aimed to employ this novel embryonic stem
    cell-like resource to promote chondrogenic differentiation using a new
    pluripotent and possibly autologous source.
     A genome-wide scan (GWAS) of 3500 osteoporotic females
    investigation: An association study of NADSYN1 gene was
    investigated using different single nucleotide polymorphisms to
    determine the effect of vitamin D serum levels and different
    mutations within the NADSYN1. This project was carried out at the
    Institute of Medical Science in University of Aberdeen.
     At the Institute of Medical Science in University of Aberdeen: The
    project involved general lab skills, cell culture and statistical analysis
    through studying the effect of drug mixtures against Candida species
    such as: Caspofungin. The drugs concentrations were compared
    against growth, cell number and other parameters to determine the
    effect and differences on fungal species.

    Skills
     Statistical analysis of cultural and genetic studies including:

    - T-test and pedigrees formation family studies and bioinformatics

    - Able to utilize statistical programs such as Microsoft Excel and Prizim


    for research analysis

     Several Lab Skills including:

    - Cultural preparation and growth media for several microorganisms


    including stem cell culturing, reprogramming somatic cells into
    induced pluripotent stem cells (embryonic stem cell-like) and
    differentiation

    - Electroporation & opto-poration transfection of DNA into tissue/cells

    - Polymerase chain reaction (PCR), Real time PCR and Quantitative PCR,
    protein purification, identification and quantification (e.g SDS-PAGE,
    Two-Hybrid system)

    - Cloning in bacteria and yeast using gel electrophoresis, north,


    southern and western blotting and DNA extraction including
    restriction aanalyses and genotyping

    - Genome sequencing techniques and library creation for multiple


    platforms including Sanger, Illumina (HiSeq, MiSeq, ISeq), Nanopore,
    PacBio and Ion torrent.

     Computer skills including:

    3
    - Microsoft office, endnote, refworks, SPSS and iWORK. Several other
    programs including MAC/Linux system programs

    - Email (outlook, Mail, Firefox) and Internet world wide web search

    - Searching scientific articles (such as Pubmed, Elsevier and others)


    and VPN extraction services

    - Bioinformatic analyses of whole-genome sequence using multiple


    assembly, mapping, SNP calling.

     Language: (bilingual) fluent in Arabic and English (listening, speaking


    and writing)

     Taught and practiced presentation and lecturing skills including


    simplifying biological and genetic terms.

     Communicative, able to work independently with minimal supervision


    and meet deadlines through time management

     Problem solver including laboratory protocols trouble shooting and


    creative thinker

     Managing and supervising molecular biology, wet and dry lab with
    high efficiency and under tight health and safety rules and regulations

    Seminars and Lectures 2001-2014


    Attended several seminars, lectures and work groups in several
    places. These may include biomedical, medical anatomy, genetic
    diseases, contribution of similar genes in different diseases and
    several other genetic, stem cell and biochemical research findings.
    They were as follows:

    - Arab Health and Arab Lab – Dubai, UAE (2014)


    - Multiple medical and educational Saudi Arabia conferences at NGHA,
    KAU (2013)
    - Oxford University (2010-2012) – several presentations
    - University of Bristol (2011) – Presented poster and accepted abstract
    - The Institute of Medical Science – University of Aberdeen (2004-2009)
    - University of Kansas Missouri (2003)
    - University of Wisconsin Superior (2001)
    - University of Minnesota Duluth (2001)

    Publications
    - Protective effect of metoclopramide against organophosphate-
    induced apoptosis in the murine skin fibroblast L929.
    Jaber BM, Petroianu GA, Rizvi SA, Borai A, Saleh NA, Hala SM, Saleh

    4
    AM

    - Abstract accepted by the British Society for Matrix Biology (BSMB):


    (2011)
    Directed Differentiation of Human Induced Pluripotent Stem
    Cells into Chondrocytes
    S Hala¹, C Yapp1, A Kramm1, L Carpenter2, S Watt2, A Carr1, J Dunford1,
    U Oppermann1, U Tirlapur1

    - Poster and oral presentation at the Annual Symposium of Stem Cell – Oxford 2011

    Human Induced Pluripotent Stem Cells differentiation into Cartilage


    under Hypoxia
    S Hala¹, C Yapp1, A Kramm1, L Carpenter2, S Watt2, A Carr1, J Dunford1,
    U Oppermann1,
    U Tirlapur1
    Botnar Research Centre, Institute of Musculoskeletal Science,
    University of Oxford

    Oral Presentation

    - Speaker at several residents and consultant research courses held at


    National Guard Health Affairs since 2015-present

    - Invited Speaker at the “prestigious” Dunn School of Pathology for research


    on Chondrogenic differentiation of Human Induced Pluripotent Stem Cells
    (2012)
    - Several presentations at the Botnar Research Centre, Institute of
    Musculoskeletal Science, Oxford University including the Annual
    research development and discussion. Further lectures, seminars were
    done at the National Guard Health Affairs – Research centre and King
    Abdulaziz University, Jeddah Saudi Arabia (2010-2014)

    - Single nucleotide polymorphisms to determine the effect of vitamin


    D serum levels and different mutations within the NADSYN1, University
    of Aberdeen (2009)

    Rewards

    5
    - High School Student of the Year (1998)

    - The Saudi government royal scholarship award (2003)

    - Prize of the top & excellent lab-group, University of Aberdeen (2007)

    - Prize for excellence of research from United Kingdom Cultural Buru (2011)

    - The Royal Prize for Science and Achievment - honored by the Saudi
    Ambassador Prince Muhammed Bin Nawaf Al-Abdulaziz for excellence in
    British and Irish universities (2012-2013)

    - Certificates of appreciation and various prizes from the MNGHA and KSAU-
    HS

    References

    Available Upon Request.

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