Human HumaStar 300
Human HumaStar 300
Human HumaStar 300
| Service Manual
System Version
Copyright
No part of this documentation may be reproduced in any form, nor processed, copied or
distributed by means of electronic systems, without prior permission of Human GmbH in
writing. Since all precautionary measures were taken into account in producing these operating
instructions, the manufacturer accepts no responsibility for any errors or omissions. This in-
cludes any liability for damage that could arise from possible incorrect operation based on this
information. Subject to changes without notice as result of technical development.
Table of Contents
1 Safety Instructions 1
1.1Introduction 1
1.2User Warranty 1
1.3Intended Use of the Instrument 2
1.4General Safety Warnings 2
1.5Disposal Management Concept 3
1.6Instrument Disinfection 3
1.7Biohazard warning 4
15 Miscellaneous 201
15.1Diluter (P/N: AY0069.05) 201
15.2Air Pump (P/N: AY0121.02) 202
15.3 Cooling System (P/N: AY0115.01) 202
15.4Thermostat (P/N: AY0131.01) 203
15.5 Maintenance 203
15.5.1 Diluter 203
15.5.2 Thermostat 206
15.5.3 Cooling System 208
15.6Trouble Shooting Guide 212
15.7 Spare Part List 213
15.8Enclosed Documentation 214
15.8.1 AY0121.00.0.SC (electrical diagram) 214
15.8.2 M0145.01.0.SC (electrical diagram) 214
15.8.3 AY0115.01.0.SC (electrical diagram) 214
15.8.4 EA0098.01.0.SC (electrical diagram) 214
Contents
18 Maintenance 267
18.1Preface 267
18.2Operating Program Checks 267
18.2.1 Reagent System 268
18.2.2 Sampling System 269
18.2.3 Reaction and Measurement System Checks 270
18.3Deviced for maintenance 272
18.3.1 To remove casing 273
18.4Photometer Module 275
18.4.1 Photometer 275
18.4.2 To Equalize and Replace Filters 277
18.4.3 Replace Motor and Belt. 280
18.4.4 Replace and clean lenses 281
18.5Temperature Adjustment and Control 281
18.5.1 Check Temperature in Incubation Bath 281
18.5.2 Check Temperature in the Reagent Chamber 283
18.6Preparation System 283
18.6.1 Sampling Arm, vertical movement (Probe) 284
18.6.2 Sampling Arm, rotational movement 286
18.6.3 Reagent Chamber 290
18.6.4 Sample Plate 293
18.7 Measurement System 296
18.7.1 Check Reaction and Measurement Plate 297
18.7.2 Cuvette Washing Arm 303
18.8Programmed Maintenance 308
18.8.1 Daily Check 308
18.8.2 Every two weeks 309
18.8.3 Once a month or when necessary 309
18.8.4 Every six months or when necessary 310
18.8.5 Replace Photometer Lamp 310
18.8.6 Programmed Maintenance Table 310
Contents
1 Safety Instructions
1.1 Introduction
This manual is considered as a part of the instrument; it has to be at the
operators hand as well as at the maintenance operators availability. For
accurate installation, use and maintenance, please read the following
instructions carefully. In order to avoid instrument damage or personal
injury, carefully read the GENERAL SAFETY WARNINGS, describing the suitable
operating procedures. In case of breakdowns or any troubles with the instru-
ment, apply to the local Technical Service.
Figure 1
Biological Hazard
Symbol
Notes:
6
HUMASTAR 300 with its sophisticated updated software offers great versatili-
ty and speed of operation. Its unique characteristics and user friendliness make
this analyzer the top in its class with its productivity and throughput of 300
Clinical Chemistry tests plus 180 ISE tests per hour.
Figure 2
BAR CODE READER built-in to identify the samples and /or reagents. (optional).
LIQUID LEVEL SENSOR: both the reagent and Sampling Probes have built-in
liquid sensors and mixer to assure a correct sample preparation.
SOFTWARE: user friendly software with graphic presentation guides the opera-
tor step by step through all operations.
HELP ON-LINE: special program to assist the operator at all times during pro-
gramming and operation.
PATIENT REPORTS: are user personalized on a 80 column printer. All result data
is automatically memorized on the built-in hard disk and available for future
consultation.
PRINT ON LINE: if activated it will print results immediately after their final mea-
surement.
GRAPHIC DISPAY: possibility to view the curve of any test for each individual
patient as well as all calibration and Quality control curves.
TEST COUNTER: displays the number and the type of test performed on the ana-
lyzer.
ARCHIVE ERRORS: a list of errors and warnings of the last 90 days are displayed.
2.3 Operation
The analyzer is a self sufficient system that uses some peripherals, such as Mo-
nitor, Mouse, keyboard and printer.
A reagent probe (with a built-in liquid level sensor) aspirates the reagent and
transfers it into the quartz reaction cuvette. The Reagent is incubated in the
liquid bath at 37C for 90 sec. During its incubation time, it is measured (this
becomes the reagent/cuvette BLANK for each test).
The Sampling Probe (with its built-in liquid level sensor and mixer) aspirates and
deposits the sample by mixing i into the warm reagent to start the reaction.
Both Sampling and the Reagent arms are automatically washed both internally
and externally, dried and are ready to prepare the next sample.
The 12 seconds operational cycle consists in:
-- A) Reagent aspiration, dispensing and measurement Reagent/Cuvette
BLANK
-- B) Sample aspiration, dispensing, mixing, a sample measurement
-- C) A final sample measurement and calculation
-- D) Wash and dry cuvette
-- E) Measurement of 20 samples in each cycle. (Each sample is measured every
24 sec 2 working cycles)
The operation is Patient Oriented. All tests for Patient ONE are prepared before
starting on patient TWO. A full patient report is printed as soon as all the result
data for a given patient are available.
All patient data as well as the analyzer calibrations and their graph are stored
on a hard disk. A built-in user friendly Help and Maintenance programs are a
perfect guide for the operator.
This warranty does not apply to damages incurred in shipment of goods. Any da-
mage so incurred shall be reported to the freight carrier for settlement or claim.
HUMAN reserves the right to reject any warranty claim on any item that has
been altered or has been returned by non-acceptable means of transportation
or packaging. In all cases, HUMAN has the sole responsibility for determining
the causes and nature of the failure, Cronys determination with regard thereto
shall be final.
12
2.5 Installation
2.5.1 Unpacking
Shipping and packing materials have been selected to provide maximum pro-
tection during transportation under normal handling conditions.
Notice: Once the carrier
has taken possession of
the system for transportati-
on from the factory, carrier
assumes all liability until
delivery. All claims for
damage due to transportati-
on must be filed with the
carrier as soon as possible.
Figure 3
Shipping crate
The system
serial number
is identified as the
serial number of
the Analyzer. Examine the shipping cartons for indications of damage, e.g. crushed or inden-
ted walls, holes or gouges, water damage, etc. Have the carrier note any such
damage on the delivery receipt; this will simplify formulating a claim if any of
the instruments or parts are damaged.
Open the carton from the top and remove the instrument with care. As shown
on the graph above. It is recommended that two people help with the removal
of the instrument from its carton and plastic bag. Save all cartons and packing
material until you are sure you have received everything and all is in good wor-
king condition.
2.5.2 Installation
The Analyzer system must be installed in the laboratory by specialized person-
nel. At the time of installation the system will be checked to ensure proper ope-
rations. During installation, at least one person in the laboratory will be trained
in operation and maintenance of the ANALYZER.
The HUMASTAR 300 a highly sophisticated, high precision, sensitive instrument.
Proper installation will ensure optimum performance.
2.5.5 Operating-Temperature-Limits
Ambient room temperature 15 C to 32 C
max. humidity 65% (non-condensing)
14
Figure 5
External Conncections
4
1 Com 1 Host 5
2 Diskette
6
3 Mouse Keyboard
4 Monitor 1
5 Com 2 Host 7
2
6 Serial Port
3
7 Printer
Figure 6
Connections in the
back of Analyzer
1. Power supply input socket (10). Make sure there is a proper GND.
2. Power Switch ON/OFF (11).
3. Power input socket for AIR PUMP (12).
4. Inlet AIR PUMP tubing (13).
5. Outlet tubing to WASTE container (14).
6. Voltage Change (15) to select voltage 220 or 110 volt.
7. Auxiliary power supply socket (16).
8. Power supply cable for the ISE module to be connected to an outside power
plug (17). The ISE module has to be always ON, since it requires continuous
auto-calibration and to keep the electrodes moist, even when the analyzer
is turned OFF.
9. AIR PUMP (18).
10. WASTE container (19), suggest to empty daily.
16
Figure 7
Hydraulic Connections
INCUBATION BATH - Fill the container with bi-distilled water and close it with its
screw cap and its level sensor.
a) When the Analyzer is turned ON, the incubation bath will be automatically
filled including its thermostat. Both the temperature and the level of liquid
in the incubation bath are continuously monitored. Missing bi-distilled wa-
ter in the bath is automatically signaled and flagged.
b) To avoid formation of bacteria or other undesirable matter inside the water
bath, it is important that every two weeks this bi-distilled water be drained
and fresh one introduced. This operation is done automatically through the
Maintenance program. Simply press first Empty and then Fill Bath. ,
2.7.1 Monitor
HUMASTAR 300 can operate with every type of monitor (CRT or LCD) as long as it
has a 15 pin VGA connector. The monitor with the help of the graphic software
will guide the operator step by step through all operations. When needed click
on (?) HELP a detailed help page will be displayed.
7
33
8
29 30 31 32 3
9 10 11 12 13
4 STAT
24 16 Place for
28
14
27
16
25 17
24 18
19 20 21 22 23
13
15
11
14 17
25
12
16
9 B
10
B 18
19
2 3 4
1 5
8
20
30
7
6
1 2
3
29
D
7
4
28
21
8
6
26 27
C
9 10 11 12
5
5
T
A
STA
22
A
ST
24 25
AND
A RD a n d
23
13
24
22
3
14
Cal.B
E
21
IS
15
C
8
16
C
20
CO
.
2
19 17 18
Primary tube
.S
25
an
N
TR
Cle
OL
9
S
26
14 10
1
13 11
12
27
40
Pre-dilution cup
28
39
38 30
D
D 29
36
32
37 34
31
Figure 9
Analytical System
Analytical plate
Figure 10
Reagent Chamber
Figure 11
Sampling Arm of
Reagent System
Figure 12
Sample Tray
The segments are available for primary tubes of 16x100 and 12.5x75 mm.
The pre-dilution cups are available from HUMAN. Code 18720/31.
Sample ID can be programmed manually using a Bar Code Reader, or an optical
pen.
The Sampling Probe has a built-in liquid level sensor to detect the presence of
the sample in its tube. Should the level of sample be inadequate, it will FLAG the
massage of Missing Sample.
22
-- Sample: from 3 to 70 l
-- Reagent: from 3ul to 500 l
-- Second Reagent: 3ul to 300 l
with increments of 1 l.
For the Reagent1 is possible to dispense lower volume to 300 l only for me-
thods PreMix and Post R2.
A peristaltic pump in series with the Diluter washes the Probes internally inside
the Washing Wells after each operation.
By means of an air gap separation the
wash solution is never in contact with
the sample.
The wash volume has been optimized
to assure perfect cleaning with less
consumption of the wash solution (2,5 L/h).
Figure 15
(17915 sample dilutor w syringe
17916 reagent dilutor w syringe
Figure 17
Peristaltic Pump
2.8 Software
The HUMASTAR 300 software has been divided into 8 parts in order you make it
easy and user friendly for the operator, all smartly enclosed in a book form.
Detailed information and procedures are described in the Operators Manual.
DOCUMENTATION
EI0110.01.0.DW (block diagram)
DOCUMENTATION
EI0107.01.0.DW (block diagram)
EI0107.01.0.SC (electrical diagram)
28
PC COMPUTER
SPS.PC
-12 VDC EA0067.02
MULTISERIAL PORT
SPS.PC
+ 12 VDC EA0067.02
PC COMPUTER
EB0046.01 PCB
REFRIGERATOR
FUSE F5 5A
MODULE
EB0043.01
AY0115.01
FUSE F1 1,6A
SPS.- 24VDC EB0054.01 PCB
+ 24 VDC 930.020.012
EB0043.01
-- + 5.0 V
-- - 5.0 V
-- + 12 V
-- - 12 V
-- + 24 V
-- 18 Vac
-- 48 Vac
-- 230/115 Vac Air
Pump
-- 230/115 Vac - AUX
DOCUMENTATION
EI0107.01.0.DW (Block diagram)
EI0107.01.0.SC (Electrical diagram)
-- Power switch
-- Primary to transformer T1
-- Secondary S4 of transformer T1
-- PC power supply
-- AUX power supply plug
-- Power supply +24 V
-- Air Pump (operated by optotriac OC1
and controlled by PCB 17970/7 )
DOCUMENTATION
17970/27.B.SC (electrical diagram)
17970/27.A.PM (assembly drawing)
30
DOCUMENTATION
EM0050.01.B.SC (electrical diagram)
DOCUMENTATION
EA0067.02.0.SC (electrical diagram)
Power supply
+23 / -23 V
Input AC J4
voltage
U1 +23 / -23 V
Output
Power supply J12 analogic
+15V voltage
U2
+15 V Incubation To
J11 incubation
bath bath
control
+15 V
U3
+12 V To
Input +12 V J3 Refrigerator J10
refrigerator
control
OC1
+24 V Analogic +24 V Output
Input +24 V J2 J8 power
power switch
SAMPLES supply
OC2
+24 V Output
Analogic
J7 power
power switch
supply
CUVETTES
OC3
Analogic +24 V Output
J6 power
power switch
REAGENTS supply
Enable
power ON
J1 Output
Enable
J9 signal for
air pump
air pump
+24 V Output
J5 for DC/DC
converter
DOCUMENTATION
17970/7 .A.SC (electrical diagram)
17970/7 .B.PM (assembly drawing)
5. To access inside the power supply, remove screws (3) and its back panel (1).
Figure 20
Figure 21
12
15
22
3B
Remove the power cord and extract the fuse container (17).
1. Disconnect connectors
Figure 22 J4 from board (5), see
Figure 20, disconnect
cables from connectors
J1, J3 from board (8), see
Figure 21.
2. Remove container (6),
take out screws (20) see
Figure 22.
3. Unscrew nut (5) that
hold the transformer (3B)
to the base (use hexa key
13mm).
4. Remove disk (3C) and the
isolation disk (3A).
5. Replace the transformer
and reassemble in re-
verse as described above.
Figure 23
1. Disconnect from the power supply (12)
connectors P1, P2, see Figure 23.
2. Remove screws (14) and remove the
board.
3. Replace the p.s. board and reassemble
in reverse. (Make sure that connector
P2 is inserted with its three red wires
on the right side of the board (12). See
the electrical diagram EI0107.01.SC).
-- Check fuse F5 F8
-- Check voltage +12V between terminals 7-8 J10, board
17970/7
-- Check +15V on terminal 8 of U3, on board 17970/7
Refrigeration in Re- -- Check voltage on TP3 < +2,75V board 17970/7, if neces-
agent Chamber does sary adjust with R48
not cool. -- Check on terminal 7 of U3 and terminal 5 of OC4 < 1V
board 17970/7, if necessary replace board 17970/7
-- Remove connector J10 and check the resistance of the
Peltier between pin 4-5 of cable WC0127.01, about 2
Ohm, if necessary replace it. (cod. EA0072.01 )
-- Distribution of signals (CK, LP, EN, CW-CCW, SYNC) to control diluter module
D2 via board 17970/11.
-- Distribution of signals (E_P2R, LP, CK) to control pump P2 via board
EB0033.03.
-- Control of pump P3 via U1, OC4 and Q4.
-- Distribution of voltage [+24 V] for pump P2 and Diluter D2.
4.1 Maintenance
Remove the outside panels: (see General Maintenance section To remove Operations to be done
outside panels). with analyzer turned OFF.
Documentation
17970/10.A.SC (electrical diagram)
17970/10.A.PM (assembly drawing)
68
1 3
SLAVE (1) controls the complete robotics of the Reagent System such as:
-- Rotation of the Reagent plate
-- Movement of the Arm with its Reagent Probe.
-- Reagent Diluter and its pump
SLAVE (2) controls the whole Analytical System (cuvette system), such as:
-- Rotation of the measuring cuvette plate
-- Rotation of the photometer filter revolver.
-- Operation of the cuvette washing Arm.
-- Pump and valve.
SLAVE (3) controls the complete robotics of the Sampling System, such as:
-- Sample plate rotation.
-- Movement of the Sampling Arm and its Probe.
-- Sample Diluter and pump
The board 17970/9 (mother board) holds also the A/D converter board
(17970/19).
74
Table 4
Item Test point Ranges
1 TP2 From + 4,75V to + 5,25V
Nominal voltages
2 TP4 From +11,0V to +12,5V
3 TP6 From +4,90V to + 5,10V Reg. PR1
All voltages are referred to GND-A = GND-D = TP2 = TP3 (with JP1 closed)
DOCUMENTATION
EB0045.00.B.SC (electrical diagram)
17970/8.A.PM (assembly drawing)
The Tables below lists the signals of the three CPU slaves.
41 21C P92
42 21A PA0
43 22C PA1
44 22A PA2
45 23C PA3
46 23A PA4
47 24C PA5
48 24A PA6
49 25C PA7
50 25A PB0 E_DCA Enable Diluter D1 Sam-
ple
51 26C PB1 E_P1CA Enable Pump P1
52 26A PB2 E_BRCA Enable Sampling Arm
53 27C PB3 E_PICA Enable Sample Plate
54 27A PB4 D_LOWCA Low power motor Diluter
D1
Low power motor Pump
55 28C PB5 P1_LOWCA
P1
Low power motor Sam-
56 28A PB6 BR_LOWCA
pling Arm
Low power motor Sam-
57 29C PB7 PI_LOWCA
ple Plate
58 29A PC0
59 30C PC1
60 30A PC2
Low power motor Sam-
61 31C PC3 PR_LOWCA
pling Probe
62 31A PC5
63 32C PC6
64 32A PC7
P20
P90
P91
P93
PC4 BEEPER Acoustic Signal
82
V V
Figure 24 7 ms 0,5
0,25 s 0,005 = 4MHz
Clock Signal U9, terminal TP6
LLH
+ 2 Vpp
Figure 25
3ms 4ms
LLL
Clock Signal U3, terminal TP5
T T
Table 7
Item Test point Range
1 TP2 From + 11,0V to + 12,5V
Nominal Voltages
2 TP3 From + 4,75V to + 5,25V
3 Pin 1 J24 From + 22V to + 24V
4 Pin 4 J24 From - 22V to - 24V
Figure 27
LED Color Function
DL1 Green [ + 12 V]
Visible Liquid Level Sensor
DL2 Green [ + 5 V]
voltages
DL5 Red [TB HIGH]
DL4 Green [TB LOW]
DL7 Yellow [WASH]
DL6 Yellow [AWASH]
DOCUMENTATION
17970/9.A.SC (electrical diagram)
17970/9.B.PM (assembly drawing)
6.3 Maintenance
Remove the outside panels. (see General Maintenance section Removal of Operations to be done with
outside panels) analyzer turned OFF.
In block diagram are shown the two timing of (HOME and SYNC) that determine
the mechanical position of ZERO. This is when the HOME signal is on logic level
LLH and signal SYNC is on LLL.
98
Figure 29
Item Test point Range
1 Pin 1 J2 From +23,5V to +24,5V
Nominal Voltages
2 Pin 20 U1 From +4,75V to +5,25V
From +690 to +710mV Reg. PR1 (with
3 Pin 15 U2
piston moving)
Da +90 a +120mV (with piston in stand
4 Pin 15 U2
still)
Figure 30
JP1 Closed
JP2 Closed
Setting jumpers
JP3 Closed
DOCUMENTATION
17970/11.A.SC (electrical diagram)
17970/11.C.PM (assembly drawing)
7.1 Maintenance
Remove the outside panels. (see General Maintenance removal of outside
panels)
Figure 31
TECHNICAL DESCRIPTION
Containers AY0113.01 and AY0114.01 hold the modules that control the stepper
motors. On the side of AY0114.01 is inserted the power supply board for the
photometer lamp.
DOCUMENTATION
17970/26.A.SC (electrical diagram)
17970/26.A.PM (assembly drawing)
We highly suggest
NOT to modify the
positions of the mo-
dules. When replac-ing -
use modules with the
same codes indicated in
front of each module.
Hardware Configuration
Table 9 indicates for each board, the calibration voltage, and position of jumpers.
Description Low
Range Test Current Setting jumpers JPX
Version Adj. power=STAND-
mV point (A) O=Open, C=Closed
BY
1 2 3 4 5 6 7 ADJ. CURRENT
Table 9
400 0,4 PR1 Low power
EB0092.02 O O O O O O O
800 0,8 PR2 work
D o 250 0,25 PR1 Low power
EB0092.03 O O O O O O O
NOT 800 0,8 PR2 work
change 100 0,1 PR1 Low power
EB0092.04 TP3 O O O O O O O
the positi- 500 0,5 PR2 work
on of the
100 0,1 PR1 Low power
EB0092.05 O O O O O O O
800 0,8 PR2 Work
jumpers.
400 0,4 PR1 Low power
EB0092.06 O O O O O O O
1000 1 PR2 work
DOCUMENTATION
EB0092.01.A.SC (electrical diagram)
17970/20 - 23.A.PM (assembly drawing)
DOCUMENTATION
18720/8.A.SC (electrical diagram)
18720/8.A.PM (assembly drawing)
128
+ 2 Vpp
Figure 36
JP1 Closed (soldered) Position jumpers
The system is shown fig. 36, aligned on an optical axis. The light produced by
the halogen lamp (UV NO STOP range from 340 to a 800nm) passes through an
optical lens, and focused on the interference filter. The monochromatic light is
focused on to the quartz measuring cuvette containing the liquid to be analyzed.
The intensity of light is then measured by a solid state detector and converted
into an analogical signal proportional to the intensity of light received. This si-
gnal is further converted into a digital result and processed by a -processor.
Figure 38
9.4 Maintenance
Remove the outside covers. (see instruction in 18 Maintenance).
P2
P1
P5
P4
Documentation
To avoid malfunction, do NOT change the positions of these pumps. When chan-
ging a pump or a PCB make sure to it has exactly the same identification number
indicated on the on the board or on the pump assembly.
Table 11
Board Description Device Layo.Ref. Software P/N
EB0122.01 Driver pump COP8SAA716 U1 PD0024.01
List of programmable
devices
DOCUMENTATION
10.2 Maintenance
Operation to be done with Remove the outside panes (See 18 Maintenance )
analyzer turned OFF.
11 Encoder Motore
TECHNICAL DESCRIPTION
Function of this board is to verify the correct operation of the stepper motors.
Table 12
Version Assembly Position of jumpers JPX
Description
Encoder Mechanical O=Open, C=Closed
JP1 JP2 JP3
(ADL) (ADH) (TST)
Encoder vertical move-
EA0044.01 AY0105.01 O O O ment of Probe
SAMPLE and REAGENT
Encoder rotational Arm
EA0044.02 AY0168.01 C C O
movement REAGENTS
Encoder rotational Arm
EA0044.02 AY0169.01 C C O
movement SAMPLE
LED Color
DL3 yellow Synchronizing Signal
DL5 Green Signal transmission serial data
DL6 Red Serial clock signal
Manufacturing Flow
chart for Encoder
assembly
EB0072.XX
or
EB0120.XX
PCB
U1=PDXXXX
jumpers
Programmable
devices
Join position
Electronics Assy
EA0044.XX
or
EA0076.XX
Mechanical Assy
+
The flow chart on the side, step motor
shows the sequence of the
encoder assembly.
AYXXXX
-- When the signal SYNC (D) coincides with the external signal HOME (H), this
becomes the correct mechanical ZERO.
-- Data is sent via a serial line SCL (serial clock) and SDA (serial data), from the
controller (U1) to the corresponding CPU slave board.
-- The red LED (DL6), indicates the presence of a signal from a serial clock (SCL),
requested by the controller (U1).
-- The green LED (DL5), indicates the presence of a serial signal (SDA) which
contains data and the peripheral address, requested by the controller (U1).
-- The timing graph as indicated in the block diagram and in the Table the two
signals SYNC (S) and external HOME (H) are shaped between them in logic
AND (D). Both LED, DL3 and DL7 will be continuously ON only when the two
signals are LLH which corresponds to the mechanical ZERO position.
160
Signal Table
In Out
DO NOT change the po- HOME (H) SYNC (S) OUT (D)
sition of the jumpers.
LLL LLL LLL
LLL LLH LLL
LLH LLL LLL
LLH LLH LLH
To see assembly se-
quence, go to the previ- HARDWARE CONFIGURATION
ous paragraph. Table 12 indicates the position of the jumpers present on the board
LED Colore
DOCUMENTATION
EB0120.00.0.SC (electrical diagram)
EB0120.01.0.PM (assembly drawing)
162
11.2 Maintenance
Remove the outside covers. (See 18 Maintenance)
Figure 39
Documentation
17970/31.0.SC (electricaldiagram)
17970/31.0.PM (assembly drawing)
TECHNICAL DESCRIPTION
-- This device an (Optical switch) is used in several modules to detect the cor-
rect mechanical position of HOME or SYNC. These have two levels LOW or
HIGH (LLL o LLH) namely (0 / +5 V).
DOCUMENTATION
EB0086.00.0.SC (electrical diagram)
EA0071-75.00.0.SC (electrical diagram)
13.1 Maintenance
To replace these parts see General Maintenance.
180
DESCRIPTION
The computer module (see the block diagram) consists of a passive back-
plane board (17810/7), a CPU Master board (17889) and a multi-serial board
(910.002.031).
1 4
1 VGA Motor
2 COM1 Host 5
2
3 Mouse Keyboard
6
4 COM2 - ISE 3
5 Printer
6 Serial Multiport
3 H.D.D. 2
4 Serial Multiport 3
5 F.D.D.
Internal Peripherics
-- OUTPUT VIDEO
-- CONTROLLER for H.D.D.and F.D.D.
-- SERIAL PORTS COM1 COM2
-- PARALLEL PORT LPT1
4 1 CN1
5
1 6 2 S1
2 3 J1
4 J3
5 J5
6 J6
7
7 S2
3
Settino jumpers
ATTENTION ! a WRONG configuration could DAMAGE the CPU.
DESCRIPTION
DESCRIPTION
Supplies to the CPU Master board four serial ports, furthermore communication
with the three CPU SLAVE boards and the Bar Code reader.
Position of jumper
JP1 Open
1. Turn ON the analyzer and wait for the boot procedure. The display will show
the memory control and some information about the BIOS. Press DEL or
CANC on the keyboard to enter the Main Menu of SETUP, the following
image will be displayed:
2. Select STANDARD CMOS SETUP and enter the parameters as shown below:
When finished introducing the data, press ESC to return to the Main Menu.
192
4. Select CHIPSET FEATURES SETUP and enter the parameters as shown below:
When finished introducing the data, press ESC to return to the Main Menu.
A. Saving SW data
B. Preparation HW
C. Update SW
D. Restore HW
E. Modification set up SW
Necessary Devices: i:
-- CD-R type reader EIDE-ATAPI
-- Power supply Cable for the CD-R reader
194
14.5.2 Preparation HW
The following proce- 1. Make sure that the Hard Disk has been preset as MASTER with the right jum-
dure cannot be done pers and the CD-R reader as Slave.
using CD or DVD masteri- 2. Connect the CD-R reader to the flat cable.
zers. 3. Connect the power supply cord to the CD-R reader.
4. Turn ON the Analyzer
5. Press DEL or CANC to enter the BIOS setup.
6. Select BIOS FEATURES SETUP
7. Select BOOT SEQUENCE, use PgUp and PgDown to select CDROM, C, A,
then press ESC to return to the previous page.
8. Insert the CD-Rom with the new software version into the CD-R reader.
9. Select SAVE and EXIT, confirm with Y. Press Enter. The PC will execute
a boot.
14.5.3 Update SW
1. As soon as the boot is over, the Start Up program will be executed from the
CD-R reader. During loading the following massage will be displayed: Gene-
ral failure writing drive A: press key F (Fail) to proceed.
2. Once the program Drive Image started, select Restore Image
3. Select Browse
4. Select file XXXXX.PQI
5. Press OK
6. Press Next
7. Select Drive C as destination to install the file.
14.5.4 To Restore HW
1. Remove the CD-R and its power supply cable.
2. Turn ON the Analyzer.
3. Press Delete to enter the Bios setup.
4. Select BIOS FEATURES SETUP
5. Select BOOT SEQUENCE, use PgUp and PgDown to select C, A, CDROM,
and press ESC to return to the previous page.
6. Select SAVE and EXIT, confirm with Y, and press Enter. PC executes a
boot
14.5.5 To Restore SW
1. When the monitor displays the first green image of Windows, press CTRL
until all of the desktop Windows has been loaded.
2. Using Service Disk execute program SAVEDBCHEM.EXE to install the Me-
thods, Controls, Calibrators, Standards and Profiles previously programmed.
3. Double click on Analyzer to execute the program.
14.6 Maintenance
Remove the outside panels (see General Maintenance)
The below operations have to be done with Analyzer turned OFF.
5. Replace the board and reassemble using the operation in reverse. (Make sure
that the flat cables are properly connected to the HD and the FD disks).
15 Miscellaneous
In this section are described some of the modules integrated in the Analyzer.
TECHNICAL DESCRIPTION
TECHNICAL DESCRIPTION
Connections:
-- The Air Pump is controlled by the
analyzer via a socket in the back of
the analyzer AIR PUMP.
-- The air tubing is inserted into the air
inlet marked AIR in the back of the
analyzer.
The power plug in the air pump contains a fuse box with two fuses. The fuse box
serves also to change the power supply voltage to either 115V or 220V.
DOCUMENTATION
AY0121.00.0.SC (electrical diagram)
EM0145.01.0.SC (electrical diagram)
TECHNICAL DESCRIPTION
DOCUMENTATION
AY0115.01.0.SC (electrical diagram)
DESCRIPTION
DOCUMENTATION
EA0098.01.0.SC (electrical diagram)
15.5 Maintenance
Remove the top panels. (see the procedure in General Maintenance)
15.5.1 Diluter
At the end, check the Diluter operation using the Diagnostic program.
Every 12 months place a few drops of very light oil on the screw (15)
19
15.5.2 Thermostat
Operation to be 15.5.2.1 To empty the hydraulics of the Thermostat and incubation bath
done with Ana- This operation can be done using the Analyzer Maintenance (F8) or the Dia-
lyzer turned ON. gnostic Tester.exe.
Turn On the Analyzer, wait approximately 30 min. to reach its optimum working
temperature.
See Figure 38
Figure 41
210
Make sure that the fan (8) inside the Reagent chamber works peoperly and cool
air is pumped in the direction of the arrow. Make sure fans (22) are in operation
and are pumping the hot dissipated air in the direction of the arrows.
Figure 42
Figure 43
218.230.210 Spiral nut for screw(order together with MS0551.01) 1 HUMAN suggests
218.152.068 Syringe belt 1 to keep in stock the
AY0121.02 Air Pump 1 parts marked with
756.040.015 Pump motor 1 (). When ordering
EM0145.01 Power supply transformer 1 parts do not forget
615.020.105 Input socket 1
to mention: Code
680.011.212 Fuses 5x20 1,25A for 230Vac 2
Number, Descripti-
680.010.216 Fuses 5x20 1,6A for 115Vac 2
on and Quantity.
AY0115.01 Complete Cooling Module 1
EA0072.01 Peltier device complete with fan 1
EA0053.02 Fan assembly 1
AY0131.01 Complete Thermostat assembly 1
EM0052.01 Heating element 1
EA0098.01 Temperature sensor 1
MA0064.01 Syringe complete - 500 l (1)(2)(3)(6) fig. 13.5-2 1
MA0064.02 Syringe complete 1000 l (1)(2)(3)(6) fig. 13.5-2 1
17917 Syringe holder 1
17915/1 Syringe with piston - 500 l (1 and 2) fig. 13.5-2 1
17916/1 Siringe with piston - 1000 l (1 and 2) fig. 13.5-2 1
17915/2 O-ring 1
MS1071.01 Piston fixing knob 1
MS1072.01 Isolation washer 1
214
DOCUMENTATION
HY0012.01.D.CM (Hydraulic diagram pag. 1 out of 4)
HY0012.01.D.CM (Hydraulic diagram pag. 4 out of 4)
HY0012.01.D.CM (part list pag. 2 out of 4)
HY0012.01.D.CM (part list pag. 3 out of 4)
In the next section is described the maintenance operation of the parts descri-
bed in section 14.1 4, with the exception of Thermostat and the AIR pump. For
details on those two items see section 13.
16.5 Maintenance
Operations described in Remove the outside covers of the analyzer: (see section 18 Maintenance).
this section have to be
done with Analyzer turned
OFF. 16.5.1 General RuLes
For maintenance both ordinary and special, please refer to the General Mainte-
nance in this section as Preventive Maintenance.
For a proper operation of the Analyzer the following maintenance operation
have to be done:
-- Replace all peristaltic pump tubing, change photometer lamp, change sam-
pling syringe.
-- Clean the incubation bath and the thermostat as described in the mainte-
nance Table.
-- Check the recycling inside the incubation bath, pump P6 and Valve V4. Make
sure that both the filling and the drain of the incubation bath are done pro-
perly within the 2 to 3 min, if necessary change the water filter at the ent-
rance to pump P6.
Figure 46
224
1. Remove tubing [16] from the washing Probe and place it into a 100 ml gra-
duated cylinder.
2. Click on [PWR ON] reaction cuvette section.
3. Click on [P5] [] to start pump and check the time to fill 50 ml of water.
4. To change the pump volume, remove the pump ( see above Replacement of
a Peristaltic Pump) and adjust the potentiometer PR2 (board EB0033.01). To
increase turn clockwise. Reassemble the pump and verify the new pump set-
ting.
When checking or replacing the tubing, make sure that the volume pumped is
set to 50 ml of water in 90 sec 2 sec.
Figure 47
Figure 48
The pump coil is polarized, pay attention when connecting the two terminals.
See the image on the side.
MS0885.01 Manifold 1
17933/3 Rotor for pump P3 P7 P8 3
17 Test Program
17.1 Description
The Test Program guides the technician to trouble shoot for a possible error or
problem. This particular Diagnostic Program (independent of the maintenance
operating program) is especially dedicated for the technical service engineer.
Each analyzer part or module can be checked and tested to assure its correct
operation. Ideal for a programmed maintenance as well as in routine trouble
shooting for errors or breakdowns.
This program enables to check, control and verify the following functions:
MESSAGES:
The analyzer generates two types of massages, one is a simple FLAG the other is
a more serious a Warning:
1. Tester.exe
-- Diagnostic Program
2. PrintMeth.exe
-- Program enables to extract ones personal data such as Methods, Profiles,
Standards, Calibrators, Controls and QC memorized data from Data-Base
onto a floppy diskette or to the hard disk
3. Satsmith.exe
-- Utility Program to enable and disable the automatic start up of Windows,
to change password and to activate the Bar-code Reader
4. SaveDbChem.exe
-- Utility service to save and to reinstall ones personal data, such as Me-
thods, Standards, Controls, etc.
SECURITY PRECAUTIONS: One should be very careful when using the diagnostics,
especially when testing ARM movements (keep hands out)
Below are described some of the important notes that are common to many of
the tests.
238
Note (1): All movements have a fixed timing for execution, beyond which they
are stopped.
To repeat a test it is necessary to reactivate PWR ON.
Whenever there is a problem, such as a motor loosing steps, or finds
friction or an obstacle, the system automatically disables the move-
ment by interrupting the power supply of +24 V. The error is shown on
the TESTER display on the top left.
Note (2): Before executing a movement (Probes, Arms or plates) make sure to
bring the PROBES into HOME position. The test does not control in-
consistent commands.
The first operation is to activate PWR ON to supply +24V to the circuits, with the
same command are transferred to the CPU slave, the Test parameters for the
Reagent System.
IMPORTANT: The tests can be run only after all the moving parts of the Reagent
System are in position HOME.
Each time a command is given, the particular name of the System becomes RED,
in this case Reagents, at the end of the test, it returns to be black.
Once a Test has been programmed, no other commands will be accepted, in any
case avoid to activate other tests, not to create malfunctions.
7. In window Pos.mm/10 and Vol.l are shown the values found as shown
in figure below.
8. To repeat the test click on [HOME Z] and then on [GO Level]
9. Make sure that the values obtained are within:
400 30 for Pos.mm/10 and ,
and 40 5 for the volume Vol. l
10. To end the test click on the grey zone.
11. At the end of the test, click in sequence [HOME Z], [HOME Plate] and [HOME
Arm] to position Reagent Arm into HOME.position.
First of all activate PWR ON the +24V, the same command transfer to the Test
parameters of the CPU slave Reaction Cuvette-measuting System.
During this phase, no
other commando will be IMPORTANT: These tests can be run only when all movements of the System are
accepted, however avoid to in position HOME.
activate other tests not to
create malfunction. Each time a command is activated the area will become red, at the end of the
Test it will return to black.
17.3.3.1 Photometer
This test is to control:
-- Lamp alignement.
-- Control of filters and photometer stability.
-- Control of the ADC converter
Click on [Photometer] a window opens below the cuvette test, as follows:
The reading scale of the converter goes from a decimal value (called count) of 0
(zero) to 65.535 (16 bit).
Items [Read One] and [Read All] will be activated only after having executed the
command [Water B.]
To start, activate PWR ON +24V for the circuits, with that command are also
transferred to the CPU slave, all the Test parameters for the Sampling System.
IMPORTANT: The tests can be run ONLY when all the moving parts are in HOME
position.
Each time a command is activated the Sysyem area Samples will assume a
RED color, at the end of testing i twill return Black.
250
17.4 PrintMeth.Exe
Program to save the personal data such as Methods, Standards, Controls, Pro-
files and the Qualit Control data onto a floppy disdette or onto the hard disk
of the analyzer.
If necessary, the saved data can be printed.
Start Procedure
1. Turn On the analyzer and wait for the boot procedure, when the first image
of Windows is displayed, press CTRL until the desktop of Windows is dis-
played.
2. Insert the HUMASTAR TOOLS diskette into drive A and follow instructions:
-- Double click on the icon floppy A
-- From the open window select PrintMeth.exe
254
Double click on PrintMeth.exe and wait until the program is loaded. After a
few seconds the image below will be displayyed.
Press Execute to save the data. After each saved file a confirmation will be
requested. When all the data is being saved, the following files are going to be
memorized.:
-- Controls.txt
-- Methods.txt
-- Standards.txt
-- StdTest.txt
-- CtrlHistory.txt
-- CtrlTest.txt
-- CtrlTestDetails.txt
The memorized file format is TXT, and some of the foles are displayed as follows:
File controls.txt
Name,ID,Lot,Expire,Code,
ContrNormal,,240 SNCM,31/08/04,9,
ContrPath,,236 SE,31/05/05,10,
File standards.txt
Name,Lot,Expire,Code,Master,Point,ISE,
[ISE Cal. B],112233,08/10/05,70,Falso,0,Vero,
Alb,01,,150,Falso,0,Falso,
Calcium,def,08/09/10,63,Falso,,Falso,
CalNormal,ghi,31/12/10,43,Falso,,Falso,
Chol,02,,151,Falso,0,Falso,
To print these files in a more legible form, open the files in EXCEL, and insert the
following parameters:
a) Select the type of file that is best for the data.:
Select: DELIMIT
b) Select the COMMA type that is better for some of the data.
Select: COMMA
256
Adapt the columns and the lines to fit the whole data comfortably, then activate
print.
17.5 SatSmith.exe
This program enables to activate and de-activate some special functions of the
analyzer and to modify some specific parameters.
Doubleclick on SatSmith.exe and wait until the program is loaded, and the
image on the right is displayed.
The colored options are those presently in use. The display shows eight fuctional
areas:
Program Features
-- LockSystem: Disables the specific keys on a Windows keyboard, including
the combination of more keys.
-- ShutDown: Enables the automatic switching OFF of the analyzer at the
end of a working cycle.
-- AutoRun: Enables the automatic START of Clinical Chemistry program.
Password
-- Password: Enables to change the password to access expert mode.
Barcode
-- Barcode: Activates de-activates the Bar Code Reader.
Protocol
-- Protocol: Activates the transmition ASTM protocol.
Level Settings
-- Level Settings: To vary the sensitivity of the liquid level sensor of the
Probe. Maximum sensitivity is LOWEST,and minimum is
HIGHEST (do NOT change).
17.6 SaveDbChem.exe
With this program enables to activate the program to save the the personal
such as Methods, Standards, Controls and Profiles that are memorized on the
analyzer into a temporary Data Base (file in MDB format), onto a floppy diskette.
This file can be used to update the Data Base on all analyzers using the same
revision.
Click on SaveDbChem.exe and wait until the program is loaded and the below
image is displayed:
The graphic image on the right enables to SELECT the four options:
-- Methods
258
-- Controls
-- Standards
-- Profiles
The Host computer that is connected to the HUMASTAR 300, has to have its
transmition parameters preset. (default values) as follows:
19200, N, 8, 1, XON/XOFF
17.7.3 Connections
The HOST computer has to be connected to the serial port 1 of the HUMASTAR
300 (COM 1), by a serial cable with the following connections:
Prologue Packet: sends first a file indicating the number of lines that will be
transferred. Followed by the structure of that data packet:
HeadChar + + NumberOfLines + + CheckSum + CR
P 2 0122
Structure example:
Prologue HeadChar + + 2 + + CheckSum + CR
Data HeadChar + + DataRecord + + CheckSum + CR
Data HeadChar + + DataRecord + + CheckSum + CR
Practical example:
P 2 0122 (sent a worklist, with two patients and a checksum of 0122)
Example
P 27 0139
P # 1|Rossi|Mario|48|1||Ematologia|0|ASTBilBicCREATGLUC 189E
P 38489|||18|1|||2|ALTASTCREATIRON 0FF8
P # 3|Bianchi|Antonella|14|0|||0|ALTASTCHOLGGTTRG 1748
P # 4|||18|0|||0|ASTBilBicCREATGLUC 1093
P 38490|||18|0|||0|ClKNaALBALB/GLBUNT.P. 1798
P # 6|||18|0|||0|ASTBilBicCREATGLUC 1095
P # 7|||18|0|||0|ASTBilBicCREATGLUC 1096
P # 11|||18|0|||0|ALTBilFixBilVArGLU2R 11C5
P # 12|||18|0|||0|ALTBilFixBilVArGLU2R 11C6
P # 13|||18|0|||0|ALTBilFixBilVArGLU2R 11C7
P # 14|||18|0|||0|ALTBilFixBilVArGLU2R 11C8
P # 15|||18|0|||0|ALTBilFixBilVArGLU2R 11C9
P # 16|||18|0|||0|ALTBilFixBilVArGLU2R 11CA
P # 17|||18|0|||0|ALTBilFixBilVArGLU2R 11CB
P # 18|||18|0|||0|ALTBilFixBilVArGLU2R 11CC
P # 19|||18|0|||0|ALTBilFixBilVArGLU2R 11CD
P # 20|||18|0|||0|ALTBilFixBilVArGLU2R 11C5
P # 21|||18|0|||0|GLUPRE 08E5
P # 22|||18|0|||0|GLUPRE 08E6
262
P # 23|||18|0|||0|GLUPRE 08E7
P # 24|||18|0|||0|GLUPRE 08E8
P # 25|||18|0|||0|GLUPRE 08E9
P # 26|||18|0|||0|GLUPRE 08EA
P # 27|||18|0|||0|GLUPRE 08EB
P # 28|||18|0|||0|GLUPRE 08EC
P # 29|||18|0|||0|GLUPRE 08ED
Where:
P # 1|Rossi|Mario|48|1||Ematologia|0|ASTBilBicCREATGLUC 189E
P + spazio: start of DataRecord (space = ASCII 32 - HEX20)
# 1: identification of sample (max 13 characters)
Rossi : First name (max 16 characters)
Mario : Second Name (max 12 characters)
48 : Age
1 : Sex (0 = Female 1 = Male)
Empty field : Time entered
Hematology : Comments (max 22 characters)
0 : Type of sample (see table)
: start Test name ( = ASCII 176)
AST : Test name (max 6 characters)
: End Test name ( = ASCII 167)
189E : Checksum hexadecimal
CR : Carriage Return ( ASCII 13 - HEX 0D )
Each area has to be separated by the symbol | (ASCII 124 - HEX 7C)
(1) Can be empty only if there is a Last Name, however, if the data is to be
memorized in the Pending List it has to have a valid ID code.
(2) Can be empty only if there is a valid ID Patient..
Example:
R # 1|Rossi|Mario|48|1||Ematologia|0|BUN|0|10.2|mg/dl|15|50|200406201030
189E
where:
R + space: is the start of DataRecord (space = ASCII 32 . HEX 20)
# 1 : Identified Patient (max 13 characters)
Rossi : Last Name (max 16 characters)
Mario : First Name (max 12 characters)
48 : Age
1 : Sex (0 = Female 1 = Male)
Empty field : Time entered
Hematology : Comments (max 22 characters)
0 : Type of Sample (see table)
264
18 Maintenance
18.1 Preface
This paragraph describes the step by step operations that the analyzer does
when turned ON and during its normal operating cycle.
1. Reagent System
2. Sample System
3. Measurement System
As the analyzer is turned ON, the operative program is loaded, initializing the
Hardware and performs a number of checks and controls to assure a perfect
operation of the analyzer. The operator can check each operation done in the
Maintenance Program.
The checks made are also shown in on the right below. When all controls have
been successfully done, the green START triangle will be lit. to indicate that
268
the analyzer is ready to operate, otherwise a red triangle will go ON, indica-
ting a malfunction.
Click on the arrow next to the triangle, a window will open to indicate the sy-
stem with the malfunction. In the Maintenance Program (F8), the malfunction
will be indicated in red WARNING in the column Result. Once the malfunction
has been eliminated, the analyzer remains in a Standby position and ready to be
programmed for the daily workload.
To monitor the correct operation between the user interface and the modules, a
check is made every 3 min. and visualized as follows:
HH.MM.SS Auto - check OK.
To access the control menu, go to the Maintenance Program (F8).
To make a check, click on its CHECK BOX and then clock START
Before performing the requested check, an additional consensus will be reque-
sted.
It is possible to request several checks at the same time, they will be performed
in the sequence they have been ordered by the CHECK BOX .
To perform checks:
All checks can be easily performed without a problem, however, special attenti-
on is needed when checking and/or activating the Peristaltic Pumps.
In order not to create malfunctions when activating the pumps, proceed as fol-
lows:
-- Position the Reagent Arm above the WASHING WELL (WASH), if necessary
activate command [Arm on Wash].
-- To expel the wash solution, activate pump P7 in the Cuvette Measurement
System [Pump P7 ON].
1. [Test Pump P2]: Pump P2 will operate for about 2 seconds. The pump will ex-
pel via the Probe the quantity of liquid it aspirated from the WASHING well.
2. [Pump P4 ON-OFF]: it will start or stop the Pump P4. The pump transferrs
Wash Solution from the Wash Container into the Washing Well.
During Analyzer initialization, the first four checks are automatically performed.
The remaining checks can be requested by the operator or when necessary in
case of a malfunction in the Sampling System with a WARNING .
270
To perform checks:
All checks can be easily performed without a problem, however, special attenti-
on is needed when checking and/or activating the Peristaltic Pumps.
In order not to create malfunctions when activating the pumps, proceed as fol-
lows:.
-- Position the Sampling Arm above the WASHING WELL (WASH), if necessary
activate command [Arm on Wash].
-- To expel the wash solution, activate pump P7 in the Cuvette Measurement
At the end of testing System [Pump P7 ON].
disactivate Pump P7 - 1. [Test Pump P1]: Pump P1 will operate for about 2 seconds. The pump will ex-
[Pump P7 OFF] pel via the Probe the quantity of liquid it aspirated from the WASHING well.
This operation has to be done every time there are problems in Calibrating the
ISE Module. If problems persist in Calibration, see 21 Optional Modules, de-
tailed description of the ISE module.
To perform checks:
All checks can be easily performed without a problem, however, special attenti-
on is needed when checking and/or activating some of the parts.
Figure 49
Table 15
CODE REFER. NUMBER. DESCRIPTION
MS1126.01 D/1 Cuvette Extractor
MS1127.01 D/2 Device to center the cuvette washing Probe
MS1129.01 D/3 Device for homing The Sampling Probe
MS1131.01 D/5 Probe to align the Sampling Arm
MS1132.01 D/6 Pin to align the cuvette plate
MC0161.01 D/7 Device to clean the Probe internally
MS1133.01 D/8 Device to align the Encoder
Device to adjust the height of the cuvette
MS1135.01 D/9
Washing Arm
MS1130.01 D/10 Device for homing the Diluter
MC0162.01 D/11 Friction device to block the cuvette plate
MC0169.01 D/12 Pin to align the encoder
Device to Insert and/or Extract Interference
MS0314.01 D/28
Filters. (design not shown)
Figure 50
Figure 51
18.4.1 Photometer
Figure 52
276
If all the values are lower then Procedure 2: lamp alignment using the Diagnostic Program Tester exe.
39.000 or higher then 60.000, The same alignment operations can be made using the Tester exe. in the Service
it is possible to adjust the sensiti- Disk.
vity of the amplifier using the PR1 18. Turn ON the Analyzer, as soon as the Window display appears, press the
of the ADC P/N EB0089.01 board. CTRL key until the program is loaded.
See Chapter 7. If one or more fil- 19. Insert the disk into drive A; click on Floppy A and proceed with program
ters are less then 39.000 or more TESTER.EXE.
then 60.000, it will be necessary 20. In section Cuvette click in succession on [PWR ON], [HOME Z], [HOME
to equalize those interference fil- PLATE], [HOME FILTER].
ters. (see 18.4.2 To Equalize and 21. Click on [Photometer] to open window Figure 45.
Replace Filters). 22. Pipette into cuvette 38 some 500 l of dist. Water
23. Click on [WATER B] to display the decimal value of transmission under the
Water B column. (the values have to be larger then zero).
24. Click to select [Filter 1] and press [Move Filter].
Table 14 displays the con- 25. Click on CHECKBOX on the right and then on [Read One] to activate the
version values between the continuous reading with filter (340nm).
different measurements. 26. Adjust knob ADJ Horizz. to maximize the value indicating in READINGS.
27. Loosen screws (3 or 4) and rotate the ADJ Vertic. (1 or 2) and maxi-
mize the value displayed in READINGS. Lock the blocking screw. If
necessary repeat the alignment to obtain an optimum value that is
between 39.000 e 60.000.
28. Click on CHECKBOX to block the continuous reading.
29. Repeat [WATER B] and check all the other 7 filters that the values
are within the required 39.000 60.000.
30. Disconnect the lamp from its power supply board and connect the
second lamp for alignment. Wait about 5 min to stabilize the lamp.
31. Repeat operations from 13 above to align the second lamp.
Figure 53
When finished, turn OFF the analyzer and reinsert the side panel
Figure 54
278
Filter nm Diskette 600 Diskette 350 Diskette 100 Cuvette 38, has to be filled
340 -- -- --
with about 500 l of dist. Wa-
380 47500 45800 10000
ter. Lamp alignment has to be
405 47500 45500 8500
510 35500 30000 4300 made with filter 340 nm. No dis-
546 35500 29000 4300 kettes are used in filter 340 nm.
578 35500 29000 4300
620 35000 285000 3700 Table 18
700 35000 31000 3500 Blockage in number of
counts for each diskette
Other wavelength
filters are available
upon request.
280
Figure 55
Figure 56
1. Remove the O-ring (12) and remove the lenses (11). Figure 47.
2. Clean the lenses and remount. Make sure to press the closing O-ring well.
Figure 57
1. Reagent System
2. Sampling System
284
Figure 58
5. Rotate the encoder disk (5) until the yellow LED (DL3) is lit on the encoder
board 17903/50. Tighten loosely screw (4) and remove spacer device (D/3).
6. Repeat the operation as described in 18.6.1.1 To Check Electronic Align-
ment and tighten the screws.
7. Replace the cover (14) and block it with screws (15). Replace the Probe with
its tube.
Reassemble everything and make sure to align the motor pulley (8) with that of
the screw. Adjust the tension of the belt and lock it with nuts (6).
At the end proceed to align the Probe (18.6.1.2 To Align the Probe into Home
Position).
Figure 59
At the end check the operation of the mixer using the Tester.exe program, by
clicking on [Mixer].
5. Click on [MIXER] and make sure that the probe does not touch the cuvette
walls.
6. Click on [HOME Z] to bring back the probe to its Home position.
7. If necessary proceed to align the Arm. (see 18.6.2.2 To Align the Arm into
Home Position).
At the end check the probe inside the Reagent bottle inside the Reagent plate
(see 18.6.3 Reagent Chamber) and inside the Sample tray in one of the sample
cups. (see 18.6.4 Sample Plate).
Figure 60
288
Operation to be done with 18.6.2.3 Replace belt and motor of the Sampling Arm
Analyzer turned OFF. The procedure is valid for both the Sampling and the Reagent Arms.
Replacement of Belt
1. Remove all connections between the Arm assembly and board 17970/20,
17970/21.
2. Remove the arm wiring from the panel (4).
3. Disconnect the Probe tubing from under the mechanical assembly.
4. Remove the four hexa screws (1) that hold the Arm assembly to panel (4).
(use a hexa key 3mm) Figure 53.
5. Loosen the screws and the hexa nuts that hold the motor (21) to the Arm
assembly. (see Figure 52).
6. Remove the belt (22) and replace it with an original one.
7. Reassemble as above in reverse.
8. Reconnect all wiring and tubing.
9. Realign the Arm (see 18.6.2.3 Replace belt and motor of the Sampling Arm)
If necessary loosen screw
(17) that holds the Opto
Home assembly (15). Reposi-
tion the sensor with the flag
of the pulley (8).
Figure 61
Figure 62
13. Pull the support (16) to tighten belt (10) and tighten screws (14) (hexa key
3 mm).
14. Rotate manually the pulley (14) to check that the ball bearing is rolling freely
and without impediments.
15. Reassemble the support (2), with screws (18), to the base (19).
16. Reconnect the wiring of motor (15) and the encoder onto the driver board
EB0092.06.
At the end check the alignment of the Reagent Plate as described in 18.6.3.2
Alignment of the Reagent Plate.
Figure 64
8. Reassemble the parts on the new motor, make sure to mount the encoder
board (1) correctly. (11 Encoder Motore).
9. To reassemble the motor proceed in reverse, make sure to align the pulleys
before tightening the screws and nuts (12) Figure 56.
10. Pull motor (15) and stretch the belt (13), tighten the screws and nuts (12).
11. Reconnect wiring to the motor (15) to the encoder board on driver board
EB0092.06 previously disconnected.
12. Realign the Reagent Chamber as described in 18.6.3.2 Alignment of the Re-
agent Plate.
Figure 65
18.6.4.4 Replace belt and motor of the Sample Tray Operation to be done with
Replace Belt Analyzer turned OFF.
1. Remove the Sample Tray.
2. Remove the large screw (6) (using a screwdriver of 7 mm) Figure 57.
3. Loosen the two screws (9) that hold the motor.
4. Remove plate (7) from the top.
5. Replace belt (8) with an original part.
6. Reassemble the plate (7) and the motor panel, without locking screws (9).
7. Pull the motor assembly (the belt should not be excessively tight) and lock
the screws (9).
8. Align the Sample Tray electronically (see 18.6.4.3 Electronic Alignment of
Sample Tray).
Replace Motor
9. Remove all connection to the motor and the encoder board (1) on driver
board EB0092.02.
10. Remove screws (9) that hold the motor panel.
11. Remove the screws and the nuts that hold the motor to its label.
12. Disassemble the motor by removing:
-- The pulley by loosening screws the screws (hexa key 1.5 mm)
-- The screws and the encoder board (1) Figure 57.
-- The encoder disk (2) loosening the screws and the spacer.
13. Re-assemble the parts on the new motor making sure that the encoder board
(1) is mounted correctly (see 11 Encoder Motore).
14. Re-assemble the motor in the reverse, without tightening the screws (9) 11
Encoder Motore.
15. Align the motor pulley with that of the plate.
16. Pull the motor to tighten the the belt (8), block screws (9).
17. Reconnect the wiring to the motor and the encoder board (1) on the driver
board EB0092.02 previously disconnected.
18. Align the Sample Tray as described in 18.6.4.3 Electronic Alignment of Sam-
ple Tray.
296
Table 17, serve to transcribe the values measured at the output of the first
stage of the amplifier, suggest to make each measurement twice.
Table 19
Position Filter nm TP2 volt Conversion A/D
1
2
3
4
5
6
7
8
Figure 67
Electronic Alignment:
1. Click in sequence in section Cuvette [PWR ON] [HOME Z] [HOME Plate] Operation to be done using
[HOME Filter]. the Tester.exe Figure 60.
2. Make sure that cuvette N1 is positioned under the Air Probe of the Washing
Arm.
3. In section Washing Arm control, enter 300 into the space mm/10 and
click [MOVE Z] to lower the Arm.
4. If the Probe touches the wall of the cuvette, proceed as below.
5. If the Probe is perfectly centered inside the cuvette, execute a photometric
measurement of all the filters, making sure that the values obtained are
within the required specs. (paragraph 18.4.3 Replace Motor and Belt.), if
necessary re-align the photometer lamp. (paragraph 18.4.3 Replace Motor
and Belt.).
6. Loosen the screw of the encoder disk inside board (16) so that the disk is
under friction (hexa key 1,5mm) Figure 67.
7. Use the pulley (45) (Figure 67) to move the Cuvette Plate to the exact cen-
ter of the Probe.
8. Rotate slowly the encoder disk until the yellow LED - DL3 and DL7 is lit on the
encoder board (16).
9. Repeat procedure from 1 to 4 as above.
10. Tighten lightly the holding screw of the encoder disk.
300
Figure 68
Mechanical Alignment
If the incubation chamber is Operatios to be done using Tester.exe
full of bi-distilled water, pro- 1. Click on [PWR ON] on all sections.
ceed with operations 1 through 3. 2. Click on Cuvette section on [TB Drain] to empty the incubation chamber.
In the contrary go to point 4. 3. When incubation chamber is empty, turn OFF the analyzer.
Operation to be done with Analyzer turned OFF.
4. Proceed with operations 2 to 4 as in 18.7.1 Check Reaction and Measure-
ment Plate.
5. Remove cuvette N38 from the plate using the extractor device D/1 (Figure
41).
6. Remove screws (30) and the photometer (11) Figure 64and Figure 67.
7. Remove screws (15) and the encoder board (16) Figure 64.
8. Loosen the encoder disk screw (hexa key 1,5mm).
9. Place device D8 in place of the encoder board (16). Rotate slowly the encoder
disk to insert the device D12 into the hole. .
10. Loosen screws (36) of the pulley (Figure 65).
11. Screw in device D/11 into the hole of the plate axis.
12. Apply pressure towards the inside to remove the quartz window from the
incubation chamber (lamp side)
13. Insert the cuvette plate and the reference pin (4), tighten knob (5) Figure
64. Rotate the plate to align cuvette 38 with the light source.
14. Insert in place of the quartz window the device D/6 aligning it with the light
source through cuvette N38. Lock with two screws TCC M3x8.
15. Position the flag (38) in the center of the Opto to Home the cuvette plate.
16. Tighten the screws (36) of the pulley Figure 67and the encoder disk
20. Click in sequence [PWR ON] [HOME Z] [HOME Plate] [HOME Filter] in section
cuvette.
21. Fill the incubation chamber with bi-distilled water by clicking on [TB Fill], it
will require two filling cycles.
22. Align the Cuvette Plate (see 18.7.1.2 Replace the Cuvette Plate and Check
Alignment).
Figure 69
Replace Motor
11. Remove screws (15) and encoder board (16) Figure 64.
12. Remove the connection of the motor from the driver board 17970/20.
13. Remove the wiring from the motor.
14. Remove the holding screws (14) and the motor assembly (hexa key 3 mm).
15. Remove screws and nuts (43) and remove the motor from the plate (42) Fi-
gure 66.
16. Disassemble the motor by removing:
-- Pulley (45) by loosening the screws (hexa key 1.5 mm).
-- Encoder disk by loosening the screw, and the spacer.
17. Re-assemble the parts on the new motor.
18. Remount the motor onto its plate (42) Figure 66, do not tighten screws
(14) Figure 64.
19. Adjust the height of the motor pulley (45) so that the belt is aligned with the
pulley (41) of the reducer (39) Figure 66.
20. Pull the motor to tighten the belt (40), tighten the screws (14).
21. Reconnect the motor to the driver board 17970/20.
22. Restore the encoder board (16) with screws (15), watch the encoder board (1)
side when mounting ( see chapter 11).
23. Proceed to the mechanical pre-alignment described in 18.7.1.5 Align the
Cuvette Plate.
1. Click in sequence, in Cuvette section on [PWR ON] [HOME Z] [HOME Plate] Operation to be done
[HOME Filter]. using Tester.exe.
2. Make sure that cuvette N1 is under the AIR probe of the of the Washing Arm.
3. In section Washing Arm control, enter 300 in the space mm/10 and click
[MOVE Z] to lower the arm.
4. If the probe touches the cuvette wall, align the Washing Arm (see 18.7.2.3
Alignment and Adjustment).
5. If both Washing Probes are centered in the cuvettes, proceed to test the ope-
ration of the Washing Station in a working cycle (paragraph 16.7.2.2).
304
To reconnect all the tubing see the hydraulic schematics HY0012.01 in Chapter
16 and Figure 63.
Mechanical Alignment
14. Insert device D/2 (with the small hole in the up position) into cuvette N1.
15. Position cuvetta N1 under the AIR probe of the Washing Arm (Home positi-
on of the plate).
16. Make sure that the support (2) of the Washing Arm, is in parallel with line
(15) of the hole on the measurement panel (9) Figure 65. If necessary
loosen screws (12) and correct the alignment.
17. Lower support (11) by moving the screw (13) (use a 3mm screwdriver ) by
means the hole into the plastic support positioned on the top.
18. Align the Probes by adjusting the screws (3).
19. Finished the mechanical alignment, check as described in 18.7.2.1 Check ali-
gnment of the Washing Arm.
306
It might be a good idea 18.7.2.5 Cleaning and Washing the inside of cuvettes
to repeat this cleaning a Operation to be done using the Maintenance Program F8
couple of times. Cleaning the Cuvettes Internally
1. Fill a clean Reagent Bottle with 40 ml of EXTRAN Neutral (or any Lab deter-
gent used for glassware) and place it into position 1 on the Reagent Plate.
2. In Maintenance (F8) from Measurement System, activate [Clean All Cu-
vettes] (18.2.3 Reaction and Measurement System Checks ).
3. The program will automatically fill all cuvettes with 500 l of the detergent,
leave it for 15 min at 37C, and then wash all cuvettes with Washing Solu-
tion.
Figure 73
Bottom View-Measurement
System
Figure 74
2 Bottom View- Measurement
System
308
Figure 75
Side View-Measurement System
-- Fill the Wash Solution Container with freshly prepared WASH solution, using
a good quality distilled water. Bad quality water generates errors in the Li-
quid Level Sensors of the Probes and consequently also in results
-- Check the liquid level in the container for the incubation chamber, if neces-
sary add.
-- If ISE Module is installed check the level of Calibrator A.
-- At the end of the working day, remove all the reagents bottles that require
refrigeration and place them into the refrigerator. When the analyzer is tur-
ned OFF, the Reagent cooling system is also OFF.
6. Run at least twice Wash all Cuvettes see 18.7.2.5 Cleaning and Washing
the inside of cuvettes.
Table 20
15 30 4 6 1 2
Operation
dd dd mo mo yy yy
Maintenance Program
Replace liquid in Incubation
Chamber
Clean Incubation chamber
Clean cuvettes
Replace Syringe type 4 and 5
(cylinder and piston)
Replace Pump tubing,
pumps P 3, P7, P8
Replace Pump tubing
pumps P1, P2, P4, P5
Replace the pump crown on
pumps P3, P7, P8
Replace pump P6
Replace Photometer lamp
Clean Optic System
Check Probe alignment
Check diluter
Check cuvettes
Check Washing Probes and
Incubation Chamber
Clean Probe Washing sta-
tion
General Cleaning and check
all pump tubing
Replace the level sensor flat
cable
312
In some cases it will be necessary to use the TESTER exe which is part of the
Diagnostic Program the HUMASTAR 300 TOOLS.
-- Same as above.
-- Check the incubation temperature
Results are LOW -- Make sure that the Reagents, Standards, Calibrators
and the Samples are fresh
-- Repeat calibration of method.
-- Check expiration date of the Serum Controls
-- Check method, it may have been programmed badly.
-- Make sure the values refer to the method and reagent
Serum Controls specs supplied by that reagent manufacturer.
are NOT within the -- Make sure that the serum controls are not deteriorated.
expected Range. -- Repeat the test using an alternative method or different
reagent
-- If substrate depletion, dilute sample and repeat calibra-
tion with freshly prepared reagents and standards.
318
Diluter does NOT start. -- Syringe piston does Not move. Check it
using the Maintenance Test.
When Syringe piston is moving,-- The moving parts need lubrification. Use a
there is a mechanical noise. tiny quantity of oil.
-- Check tubing connections and the syringe
piston
Diluter does NOT aspirate or -- Probe not connected properly
dispense -- Probe is blocked, clean
-- Wash Solution container is empty
-- Syringe and piston deteriorated.
-- Make sure there is Wash Solution in the
container.
Hydraulic tubing is not filled
-- Check for piston leaks or badly held tubing
properly.
connections.
-- Remove syringe, clean it or replace it.
-- Check piston
-- Check O-ring
Leaking Syringe -- Check the input and output of all tubing
-- Make sure the Probe is not blocked
-- Replace syringe with piston
-- Make sure that the piston is airtight inside
the syringe
Air bubbles inside the syringe -- Make sure that the Wash Solution is pro-
perly prepared, with densioactive Tween 20
(one drop per liter of dist. Water)
-- Make sure that the connecting tubing to the
syringe are not bent.
-- Bent or torn tubing, replace
Air bubbles present in tubing,
-- Check connection to the probe
syringe and probe.
-- Peristaltic pump P1 tubing is damaged, re-
place.
-- Make sure that the O-ring on the syringe is
airtight.
-- Check if the Wash Solution container is em-
Tubing connected to Probe is
pty.
empty.
-- Check tubing, if necessary change.
-- Make sure that pump P1 operates correctly.
CODE DESCRIPTION
A00740.01 Plastic sample Cups (Bag of 2000 p)
KG0046.01 Reagent Bottles 40ml (box of 50 p)
17905/S Reagent Bottles 5ml
756.020.040 Rotor pump P 3, P7, P8 tubing included
17995S/20 Adapter for plastic samples cups
MS1204.01 Adapter for bottles 5ml
17915/1 Syringe with piston N 4
17916/1 Syringe with piston N 5
17938/S Sampling and reagent Probe
17920732 Photometer lamp
17950 Optical quartz Cuvette reading (box of 5 p)
17934 Kit Pump tubing for P1, P2, P4, P5 (4 p) + P3, P7, P8 (4 p).
17916/S Teflon Tubing w/connector for Probe
17995S/1 Fuse (int.) F5H250V ( 5x20 mm) for 230 Vac
17995S/2 Fuse (int.) F8H250V ( 5x20 mm) for 115 Vac
17941/11 Water filter for incub. bath
MA0168.01 Sample Tray Sector A -110
MA0168.02 Sample Tray Sector B -1120
MA0168.03 Sample Tray Sector C -2130
MA0168.04 Sample Tray Sector D -3140
MS1047.02 WASH solution container of 5 L.
A01366.01 Container for incub. liquid. 1 L.
17941 Level sensor electrode for wash solution
AY0038.01 Level sensor electrode for incub. Liquid
KG0054.01 ISE module (complete KIT with Electrodes)
17903/12 Modulo ISE (without Electrodes)
805.004.006 Reference Electrode
805.004.008 Electrode for Na
805.004.007 Electrode for K
805.004.009 Electrode for Cl
805.004.012 Electrode control air bubbles
756.010.041 Pump for CAL A (PA)
756.010.042 Waste pump (PW)
CODE DESCRIPTION
EB0043.01 Power supply board
17970/8 CPU slave
17970/31 Level sensor
EA0097.01 Computer PC MASTER (Pentium)
17970/27 Distribution board
17995S/12 Interface pump & valve
17970/10 Power supply lamp
17970/9 Mother board
17970/11 Driver diluter
17970/26 M/B control motor
EB0099.01 M/B control motor
17970/19 ADC converter
EB0033.01 Driver step motor (pump P5)
EB0033.02 Driver step motor (pump P4)
EB0033.03 Driver step motor (pump P1 - P2)
EB0122.01 Driver pump P6
17903/50 Motor Encoder (vertical mov. sampling Probe)
17903/51 Motor Encoder (rotational mov. sampling Arm)
17903/52 Motor Encoder (rotation mov. analytical plate)
17903/53 Motor Encoder )rotation mov. Reagent-samples plate)
A00513.02 Preamplifier
EB0111.01 Bar-code interface
EB0112.01 Signals interface RS232
EA0073.02 Complete microprocessor assy (CPU slave & ADC converter)
910.002.025 Back-plane (passive 6 slot ISA bus)
17987 Serial multiport
930.020.012 Power supply +24V
17970/12 Optical sensor flag HOME (sample plate)
17970/13 Optical sensor flag SYNC (filter wheel)
17970/14 Optical sensor flag HOME (filter wheel)
17970/15 Optical sensor flag HOME (sample arm)
20.4 Fuses
CODE DESCRIPTION
PD0011.01 GAL 16V8 - U5 - EB0045.02
PD0012.02 GAL 16V8 U7 - EB0045.02
PD0052.01 EPROM - U2 - EB0045.02
330
CODE DESCRIPTION
EM0050.01 Transformer power supply
EM0052.01 Heating element
17941/2 Temperature sensor
17920/32 Photometer lamp
EA0065.01 Main switch with filter
EA0066.01 Power cord with socket for Air pump & aux
17950 Optical quartz Cuvette reading (box of 5 p)
A00740.01 Plastic sample Cups (Bag of 2000 p)
17915/1 Syringe with piston N 4
17916/1 Syringe with piston N 5
17941/3 Level sensor analytical chamber
17938/S Sampling probe (reagent & sample)
506.120.021 Preamplifier detector
CODE DESCRIPTION
677.015.005 340 nm
677.015.007 380 nm
677.015.010 405 nm
677.015.020 510 nm
677.015.030 546 nm
677.015.035 578 nm
677.015.040 620 nm
677.015.052 700 nm
674.020.005 Optical lens F=24
17920/30 Complete filter wheel (standard set)
KO0030.01 Set diskettes of blockage (three levels 600, 350, 100)
CODE DESCRIPTION
17956/1 Power supply PC
17915 Dilutor module
AY0096.01 Computer module 1 version
AY0199.01 Computer module 2 version
AY0117.01 Pumps & valves (manifold)
17930 Peristaltic pump P1 P2
17932 Peristaltic pump P4
17932 Peristaltic P5
17970/30 Complete photometer module
AY0133.01 Complete Bar-code reader (optional)
17903/S Complete power supply module
AY0131.01 Thermostat
AY0115.01 Refrigeration module
AY0172.01 Cuvettes Washing arm (1 version)
AY0200.01 Cuvettes Washing arm (2 version)
AY0121.02 Air pump
MC0123.01 Analytical chamber
MA0188.01 Analytical plate
CODE DESCRIPTION QT
MA0064.01 Syringe holder with Syringe & piston N 4 (500 l) 1
MA0064.02 Syringe holder with Syringe & piston N 5 (1000 l) 1
17917 Syringe holder 1
17915/1 Syringe with piston N 4 1
17916/1 Syringe with piston N 5 1
256.010.009 Rubber ring (O-ring) 1
MS1071.01 Fixing screw for piston (nylon) 1
MS1072.01 Isolating washer (nylon) 1
A01120.01 Connector IN pump P1-P2 2
17953 Connector OUT pump P1-P2 2
A01120.01 Connector IN pump P4 1
A01121.01 Connector OUT pump P4 1
A01121.01 Connector IN-OUT pump P5 1
MS0885.01 Manifold assy AY0117.01 1
MC0128.01 Washing station 1
Air probe for cuvettes washing arm AY0172.01 e
MC0177.01 1
AY0200.01
MC0176.01 Wash Probe for cuvette washing arm AY0172.01 1
332
CODE DESCRIPTION QT
WC0101.02 Level sensor flex cable 1
CODE DESCRIPTION QT
FC0053.01 From J6 - EB0046.01 to J5 - EB0098.01 (W14) 1
FC0053.02 From J7 - EB0046.01 to J4 - EB0098.01 (W15) 1
FC0054.01 From J5 - EB0046.01 to J4 - EB0099.01 (W11) 1
FC0058.01 From J10 - EB0046.01 to J3 - EB0098.01 (W16) 1
FC0059.01 From J8 - EB0046.01 to J5 - EB0099.01 (W12) 1
FC0059.02 From J9 - EB0046.01 to J3 - EB0099.01 (W13) 1
FC0061.01 From J2 - EB0046.01 to J3 - EB0093.01 (W17) 1
FC0061.02 From J4 - EB0046.01 to J1 - EB0093.01 (W18) 1
FC0061.03 From J3 - EB0046.01 to J5 - EB0093.01 (W19) 1
FC0066.02 From J7 - EB0093.01 to J1 - EB0068.01-D2 (W20) 1
FC0066.01 From J8 - EB0093.01 to J1 - EB0068.01-D1 (W21) 1
FC0049.01 From J13 - EB0093.01 to J1 - EB0033.01-P2 (W24) 1
FC0049.02 From J15 - EB0093.01 to J1 - EB0033.01-P5 (W25) 1
FC0062.01 From J12 - EB0046.01 to J1 - EB0043.01-P5 (W10) 1
FC0067.01 From J9 - EB0093.01 to J1 - EB0033.01-P1 (W22) 1
FC0067.02 From J10 - EB0093.01 to J1 - EB0033.01-P4 (W23) 1
20.13 Motors
20.15 BELTS
21 Optional Modules
This Chapter describes Optional Modules installation. However, installation
should be done at the factory or by specialized Crony personnel.
TECHNICAL DESCRIPTION:
Figure 76
Visible Signals
Table 21
D1 (Red) Power supply +5V
D2 (Green) Reading OK
Visible Signals
Table 22
JP1 1-2 Samples / 2-3 Reagents (see note below)
JP2 1-2
Jumpers Position
JP3 1-2
JP4 Open
Figure 77
Reagents and Samples Bar Code
Readers
11. Click on [Init] and wait some acoustic beeps that signalize the INIT of reader.
12. Click on [Scan] button, and check if the Laser beam hits exactly in the middle
the Bar Code Label of bottle 2, (see image on the right) Figure 80. If neces-
sary adjust the cams (2) (use the flat screw driver 5mm) Figure 78 to obtain
the its correct reading.
344
Figure 79
Figure 80
19. Click on [BarCode] in the area Sample. The Bar Code Test will be displayed on
the monitor, as shown on side.
20. Click on [Init] and wait some acoustic beeps that signalize the INIT of reader.
21. Click on [Scan] and observe the direction of the Laser beam, it should hit the
Label exactly in the middle, repeat the test, if necessary rotate the assay Rea-
der (1) to align it and check again the reading. See image on side.
22. Tighten the screws (use a hex key 3 and 4mm) and repeat the reading more
times.
23. In case of a wrong or missing reading, the message NR (NO reading). Its
will be displayed.
24. Click on any gray area to close the program.
When finished the testing procedure, verify again the Bar Code Readings of both
the Sample and Reagent.
21.1.3.1 Replace the Bar Code Driver Board The flex cable P/N:
Operation to be done with Analyzer turned OFF. 506.200.090 has the electrical
Remove the top panels (see General Maintenance). contacts on one side only. DO
1. Remove the screws holding the Bar Code assembly. NOT INVERT THE FLAT CABLE .
346
2. Replace the board (in case of two connectors J2 and J3, make sure to respect
the position of the flex cable to connector J2 EB0111.01.
3. Proceed to check the operation as described in 21.1.3 Bar Code Reader Op-
tical Alignment.
Figure 81
Figure 82
21.2 Documentation
Figure 83
350
Figure 84
Figure 85
352
21.3.1 Introduction
The ISE Module is an optional accessory built-in the HumaStar HS300SR analy-
zer, for the automatic determination of electrolytes. Its a compact, precise and
reliable modular accessory that compliments the system by increasing consi-
derably the throughput without complicating its operation.
The ISE module is easily built-in into the HumaStar HS300SR, for the automatic
determination of Na, K and Cl in Serum, Plasma or diluted Urine.
The ISE Module can be installed in the factory or by a qualified Crony service
engineer in the customers Lab.
The module is self-sufficient, all operations are automatic, and all operation,
data transfer and exchange are controlled by the Analyzer through a dedicated
serial line.
The ISE Module requires only a single Reagent Calibrator A - which is both one
of the calibrators as well as regenerates the electrodes. A second Calibrator B is
used as a second Standard and is located on the Sample tray together with all
the other Standards and Calibrators.
During the operative cycle, the Sampling probe transfers 90 l of sample direct-
ly into the Module. After each measurement there is an automatic wash with
Calibrator A to avoid contamination and the carry-over from sample to sample
(carryover).The Module holds four electrodes for Na, K, Cl and a Reference.
Characteristics Benefits
Integral sample entry port Minimizes sample carryover
Small Sample size Only 90 l
Minimum electronic noise,
Electrodes mounted close to electronics
improves precision.
Fast results for all 3 determina-
Rapid operation (only 30 sec. cycle time)
tions
No membranes to be serviced Simple maintenance
Maintenance done by lab perso-
Easy access to pumps
nnel
Two point calibration High accuracy and precision
One point calibration with every sample High accuracy and precision
Convenient and less mainte-
Maintenance free electrodes
nance
Adjust the voltage on TP13, 21.3.3 ISE Control Board (P/N: EB0181.02)
every time the bubbles detec- TECHNICAL DESCRIPTION
tor has to be replaced (see the This board receives the signals from four electrodes Na, K, CL and Ref, and by
procedure below) means the three pre-amplifiers converts these signals in an output continuous
voltage.
The analogical signals from pre-amplifiers are sent to a multiplexer and later to
a voltage frequency converter V/F. This latter, generates in out a square signal
with frequency proportional to its input voltage and with an amplitude of about
5V. The frequency comes sent to an input of -controller to be measured. A se-
cond input of -controller, receives the signal from an air bubbles detector for
determine the proper filling of electrodes. The structure of this board is shown
in the block diagram below.
Block diagnam
EB0181.02
12 V power
supply
Air
U1 bubbles
Microcontroller detector
DATA U3
transmission by V/F
converter
RS232 line
Na
U2 K
Multiplexer
CL
WASTE CAL.A
pump pump
REF.
Table 23 shows the voltage values of the stepper motors and the Table 24
the position of the jumpers present on the board.
All voltages are referred to AGND = DGND = TP6 = TP7 (with JP2 closed)
DOCUMENTATION
EB0181.02.0.SC - ISE module control board, Electrical diagram, pg 1-2, see Fi-
gure 83.
EB0181.02.0.SC - ISE module control board, Electrical diagram, pg 2-2, see Fi-
gure 84.
EB0181.02.0.PM - ISE module control board, Assembly drawing, pg 1-2, see Fi-
gure 85.
21.3.3.1 How changes the air bubble signal (TP13) during a purge cycle
The air bubble flag (TP13) informs the micro-controller about the proper filling
of electrodes channel, it is generated by an optical detector.
356
3. Start the ISE purge command, start the WASTE pump, the sample comes
transferred into the electrodes channel and the detector signal changes
from about 4V to < 1V.
4. During this time the electrodes channel comes filled with solution and only
when the signal of detector changes again to about 4 V, the waste pump is
deactivate. In this way the sector (A) of electrodes channel is empty, while
the next sector (B) is completely filled.
The flag output signal TP13 is adjusted by PR1, when the electrodes channel is
completely empty; its value is set about 4V.
U2 +5 V +12 V
+12 V Power
supply
U1
CK J2 TO
J3 MICROSTEPPING MOTOR
DRIVER
Q1
ENABLE
Q3
LOPWR
358
HARDWARE CONFIGURATION
DOCUMENTATION:
VERSION
PUMP PUMP ASSAY DRAWING DESCRIPTION
BOARD
Pump stepper motor drive Elec-
trical diagram, see 21.4 Documen-
EB0161.05.0.SC tation
PW AY0238.01 EB0161.05
EB0161.05.0.PM Pump stepper motor drive As-
sembly drawing, see 21.4 Docu-
mentation
Pump stepper motor drive Elec-
trical diagram, see 21.4 Documen-
EB0161.06.0.SC tation
PA AY0238.02 EB0161.06
EB0161.06.0.PM Pump stepper motor drive As-
sembly drawing, see 21.4 Docu-
mentation
Version Range (mV) Current (A) ADJ Jumpers settings JPX, O=Open, C=Closed
2 5 6 7 14 15 16
Led Color
DL1 Red +12 V power supply
DOCUMENTATION
EB0171.01.0.SC Serial adapter board Electrical diagram, see 21.4 Documen-
tation
EB0171.01.0.PM Serial adapter board Assembly drawing, see 21.4 Documen-
tation
Figure 86
Connecting diagramm
21.3.9.1 Assemblies ISE Module and Pumps To make easy the operation,
1. Loosen the screw (A) that holds the inlet sample cup (B) of module (4). its suggest before to install
2. Rotate the inlet sample cup (B), so that it has the enters tubing in direction the module on the measuring hol-
as shown in Fig. on side, then tighten screw (A). der, connect both tubing, (39) to
waste connector (4) and
(TU0175.01) to sample cup (1) see
Figure 89).
Figure 87
3. Position the module on the measurement holder plate and lock it with the
two screws (7), (see Figure 82and Figure 83).
Figure 88
362
4. Assemble the two pumps (2) and (3) on its holding panel (1) using screws (6),
to keep the right position (see Figure 82 and Figure 84.
5. Position the two pumps assay on analyzer base under the sampling tray (see
Figure 84) and lock them with the two screws (8) (see Figure 82).
6. Position the power supply (PS) on the analyzer base in the place indicated in
the Figure 84. To hold it, use two sided adhesive tape.
Figure 89
1. Insert the input cord from the power supply (PS) through the hole present on
the right side in the back of the analyzer (front view, see Figure 84).
2. Connect the output connector from the Universal Power supply to the +12 V
socket of the ISE module (see Figure 85).
3. Connect the pumps cable connectors one to the ISE module into socket
PUMPS and others on the pump drive board socket without distinction (see
Figure 85).
Figure 91
Connecting internal
serial port COM2
Figure 92
4. Insert the EB0171.01 serial adapter board, on its connector COM 2 of the
Master PC (see Figure 87).
5. Connect a side of cord RS232 to the serial port (COM2) from the Master PC
board, via EB0171.01 serial adapter board LAN-9DB.
6. Fix the cord with plastic clamp.
7. Give the other side of cord RS232 into the its inferior side through the hole
near the PC Master board, then position the two cables (RS232l and Power
out) along the right side and onward (frontal view), until to the ISE module
(see Figure 84 and Figure 85).
8. Connect the Power and RS232 cable to the ISE module connectors.
The module has two LED signals next to the electrical connectors.
-- LED Red (ON): Indicates that the ISE module is ON
-- LED Red (blinking): indicates that the ISE module is ready to receive or to
transmit data via serial line
3. Connect the output tubing (39) from the (PW) pump to the waste connector
(4) located under the ISE Module.
4. Connect the waste tubing (61) from its (PW) pump, into the internal waste
chamber connector (3) through the joint (49).
Figure 93
Hydraulics connections
Figure 94
ISE Module connections
Click on [Test ISE] button, to start the two pumps and implement a washing
cycle of ISE module, after few seconds the result will be displayed on the screen
as follows:
-- ISE Ready: its ready to work
-- ISE NOT ready: it has problems of operation, or the serial line between the
Master PC and ISE module is missing or bad (see all connections), or the signal
of the air bubble detector on TP13 of ISE control board code N EB0181.02,
does not work properly (see 21.3.3.1 How changes the air bubble signal
(TP13) during a purge cycle).
It is recommended to calibrate the ISE module daily, together with all the other
methods of clinical chemistry.
Place some 500 l of Calibrator B into a plastic sample cup and another cup with
500 of Clean S solution and place them both into their reserved places on the
Sample Tray. Use fresh cups and fresh solutions daily, DO NOT add fresh solu-
tions to the cups of the day before.
Program the calibration for Na, K, and Cl together with all the other methods
daily. We also suggest to run at least one or two Serum Controls daily, it will take
only two minutes more, but it will confirm the correct operation of the analyzer.
366
Calibration:
1. An aliquot of Clean S solution is transferred from the Sample tray into the
When a programmed calibra- module to clean the electrodes.
tion has not been successful, 2. Automatically the electrodes are rinsed and cleaned with Calibrator A and
due to a faulty electrode or mis- measured the first calibration point.
sing Cal A or Cal B. etc, the ISE Mo- 3. Finally the Calibrator B is transferred twice and the second calibration
dule cannot operate properly. In point is measured.
case: a) Only one of the three 4. It the calibration is correct (OK). A massage will be displayed with their cali-
electrodes is faulty, the analyzer bration values.
will report the values of the other 5. Should there be a problem in the first calibration, a massage will be displa-
two. b) If no daily calibration has yed indicating the problem. Repeat Calibration.
been done or programmed, the 6. Should the problem persist, refer to the Trouble Shooting Guide below.
analyzer will run the tests using
the values of the last calibration To assure the correct operation of the system and to update the Quality Control
done. In any case we highly re- Program, it is recommended to run at least one or two serum controls daily.
commend to run at least one or
two Serum Controls to assure When the ISE Module is in Standby in order to maintain the electrodes moist
that the results are correct. and ready for operation, every 30 min. a small quantity of Calibrator A is au-
tomatically aspirated.
21.3.12 Maintenance
See Figure 90, shows the module with its four electrodes.
To simplify the replacement of the electrodes, the best way is to remove the
module from the analyzer, replace the electrodes and reassemble. Fist of to re-
place the electrodes, its necessary to purge the hydraulic circuit of ISE module
as follows:
Ref
All four electrodes have to be aligned with the same distance from each other.
Make sure to hear a click when the electrode is inserted.
The electrodes installed will perform for a period of 6 months or 10,000 test.
368
Every SIX months or when ne- 21.3.12.2 ISE control board and accessories replacement
cessary, To ensure an accurate Follow these operations, if there has been a leak of liquid through the elec-
maintenance of the ISE module, trodes channel, or because some components are damaged, see Figure 91.
its suggest to wash some parts as
sample cup, waste connector with
good detergent used for laborato-
ry, then rinse everything several
time with BI-distilled water.
Figure 96
1. Fist of staring this procedure, its necessary to purge the hydraulic circuit of
ISE module for empty the electrodes channel, see 21.3.12.1 Replace Elec-
trodes, points 1-2.
2. Take out all the electrodes and air bubbles detector.
3. Remove the ISE module from the measuring holder assay of the analyzer, see
21.3.9.1 Assemblies ISE Module and Pumps.
4. REPLACE ISE CONTROL BOARD (12): remove the four screws (9) and take out
the cover (10).
5. Unscrew the four knob (11) and to extract the PCB, when remounting the
PCB make sure that the terminals of the air bubbles detector is well inserted
into the connector to six ways mounted on the PCB (12).
6. REPLACE SAMPLE CUP (3): remove screw (1), take out in sequence the holder
(2) and sample cup (3).
7. REPLACE WASTE CONNECTOR (7) and SPRING (8): remove the screws (4) (5)
to take out the lever (6).
8. To extract from the top the waste connector (7) and spring (8).
9. Reassemble in order; spring (8), waste connector (7) on support (13), than to
insert the lever (6) through the hole (14) on the waste connector (7), after fix
the screws (4) (5).
10. After having inserted this mechanics components, check the correct operati-
on of the compression spring, pressing the lever (6).
11. Once that the mechanism has been repositioned well, reassemble the eve-
rything, starting in sequence from the reference electrode than Cl, K, Na and
air bubbles detector, at end to position the sample cup with its holder (2)
then tighten the holding screws (1).
12. Proceed to check the operation of ISE module as described above in para-
graph 16.3.10.
Figure 97
6. Remove the peristaltic pumps assay from the analyzer Figure 82.
7. Remove the pump from the holder plate
8. REPLACE TUBING (6): remove the screws (1) and take off the holder tubing
(2)
9. Replace the tubing (6) with original part after having inserted the joint con-
nector (5) (pay attention to the version - see 21.3.9.4 Hydraulics parts list)
10. REPLACE ROTOR (3): loosen the screw (4) and to extract the wheel ( pay at-
tention do not to lose the spacer (10)
11. REPLACE PUMP DRIVE BOARD (8): disconnect the pump connector, than re-
move the screws (9) and take out the PCB
12. Reassemble everything following the reverse procedure
This operation will be necessary, especially if the ISE Module has not been used
for a long time or when the electrodes are dirty or old.
The test above indicates that the ISE Module is working correctly. If the above
results are not obtained, the module might need special maintenance or service.
Two possible situations can occur:
1. One or more results during calibration are not within the specified range.
-- It is possible that the electrode is old and exhausted. Next to the result that
is out of range will be added the (Error ISE).
2. None of the results are within the specified range
-- The problem can also be the Reference electrode; in that case a massage is
displayed. (Check the solution inside the Reference electrode; there is a little
marble inside that controls the salinity of the solution. If the marble is on
372
the bottom the solution is good, if the marble is floating the solution has
deteriorated and the Electrode has to be changed).
-- The problem can also be, an old or contaminated solution of Cal A and Cal B.
The ISE module can to send to the operator, 5 different errors messages:
21.4 Documentation
Figure 98
ISE module control
board - Electrical
diagram, pg 1-2
Figure 99
ISE module control
board - Electrical
diagram, pg 2-2
378
Figure 100
ISE control board -
Assembly drawing
Figure 101
Pump stepper mo-
tor drive - Electrical
diagram
380
Figure 102
Pump stepper
motor drive -
Assembly drawing
Figure 103
Pump stepper
motor drive -
Electrical diagram
382
Figure 104
Pump stepper
motor drive -
Assembly drawing
Figure 105
Serial adapter -
Electrical diagram
384
Figure 106
Serial adapter -
Assembly drawing