BR 21443 Chromatography Catalog LC BR21443 en

Thermo Scientific
1.5
1.0
Chromatography Columns
0.5
and Consumables 2016-2017
Connected chromatography
solutions
LC Columns and Accessories
As a leader in LC column technology including silica, polymer and porous graphitic
carbon manufacturing, bonded phase production and column packing for 40 years,
you can rely on the quality of Thermo Scientific HPLC products. Here, we showcase
our latest and most comprehensive range of innovative columns, accessories and
equipment for fast LC, analytical HPLC and UHPLC.
Featured Products
Thermo Scientific Chromatography Columns and Consumables 2016-2017
Accucore Hypersil GOLD

Solid core particles enabling high Thermo Scientific Hypersil GOLD
speed, high resolution separations columns offers chromatographers
with back pressures significantly outstanding peak shape for reversed
lower than those associated phase, ion exchange, HILIC or normal
with UHPLC. phase chromatography.
PAGE 4-005 PAGE 4-020
4
LC Columns and Accessories >>
Section Contents
Accucore HPLC and UHPLC Columns . . . . . . . . . . 4-005 Syncronis HPLC and UHPLC Columns . . . . . . . . . . 4-062
Hypersil GOLD HPLC and UHPLC Columns . . . . . . 4-020 Hypersil BDS and Hypersil Classical HPLC Columns 4-070
Acclaim HPLC and UHPLC Columns . . . . . . . . . . . 4-034 LC Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-078
Section Contents
Application Specific HPLC Columns . . . . . . . . . . . 4-048 LC Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-098
Hypercarb HPLC Columns . . . . . . . . . . . . . . . . . . . 4-059 LC and LC-MS Instruments . . . . . . . . . . . . . . . . . . 4-108
Acclaim Viper
Acclaim columns are based on Maximize liquid chromatography
high-purity, porous silica particles (LC) performance and get zero-
with advanced and innovative dead-volume connections to
column bonding technologies. This ensure optimal separations.
provides complementary selectivity, PAGE 4-078
high column efficiencies and
symmetrical peaks.
PAGE 4-034
www.thermoscientific.com/chromatography 4-001
4-002
LC Columns
Technical Resources
visit www.thermoscientific.com/chromexpert

to access the following information:
HPLC Column Selection
HPLC Column Selection by US Pharmacopeia Specifications
Thermo Scientific HPLC Phases
HPLC Column Selection by Manufacturer
Method Transfer
Scaling a Method Up or Down
HPLC Troubleshooting
HPLC Definitions and Equations
Selecting the Correct Buffer
Preparation of Mobile Phases
Solvent Properties
Technical Resources
Chromophore Detection Wavelengths
Column Cleaning and Regeneration
www.
thermoscientific.com/chromexpert
www.
thermoscientific.com
/chromexpert
www.
/chromexpert
Columns for Fast
and Analytical HPLC
The Thermo Scientific LC column portfolio offers a comprehensive range of high quality
options for fast and analytical HPLC and UHPLC.
The table below shows our leading column families with details of relative hydrophobicity, key attributes and how the attributes
are achieved. Use this table and the information in the subsequent catalogue pages to find the best Thermo Scientific LC columns
for your separation needs.
Hydrophobicity Key Attribute Achieved through
Accucore Columns
Page 4-005 1.5, 2.6 and 4m solid
Ultimate core silica particles
Performance C ore Enhanced
Technology
Low
Hypersil GOLD Columns
Page 4-020 1.9, 3, 5 and 12m
ultra-high purity silica
Selectivity & particles
Peak Shape
W ide range of
conventional phases
Acclaim Columns
Page 4-034 2.2, 3 and 5m ultra-pure
Traditional,
Speciality & silica particles
Medium/High
Application Innovative and unique
Specific Phases bonded phases
Syncronis Columns 1.7, 3 and 5m high

Page 4-062 surface area silica

Ruggedness & particles
High
Reproducibility Dense bonding, double
endcapping, rigorous
testing
Hypercarb Columns
Page 4-059 3 and 5m 100% porous
Extended graphitic carbon particles
Very High Separation
Polar retention effect
Capabilities
on graphite (PREG)
4-004
Accucore HPLC
and UHPLC Columns
Ultimate Core Performance
Accucore
Speed and Selectivity Combined
Thermo Scientific Accucore HPLC and UHPLC columns are a family of high speed,
high resolution columns based on Core Enhanced Technology.
Next-Generation Accucore
Vanquish UHPLC Columns
Combines the benefits of a solid core material
and the increased chromatographic efficiency of
a sub-2m particle
Solid Core Particles

With a solid central core and porous outer layer,
these particles generate high speed, high resolution
separations without excessive backpressure
Tight Control of Particle Diameter

Enhanced selection process keeps particle size
distribution to a minimum and produces high
efficiency columns
The Accucore web page contains the latest news, applications

and downloads for the Accucore HPLC and UHPLC column range.
Visit www.thermoscientific.com/accucore
Accucore Vanquish
Powerful separations are our core performance
Thermo Scientific Accucore Vanquish UHPLC columns provide a robust

chromatography solution to enhance laboratory workflows and productivity.
These columns, in combination with Vanquish UHPLC systems, deliver
powerful separations to solve your analytical challenges faster and
more effectively.
These next-generation UHPLC columns feature 1.5m solid core particles
and combine the benefits of a solid core material and the increased
chromatographic efficiency of a sub-2m particle.
Modern analytical laboratories continue to be driven towards higher
throughput workflows which require better separations, more results
and easier interaction at a reduced cost. Accucore Vanquish UHPLC
columns enable you to achieve this by delivering:
Better separations
The high efficiency offered by Accucore Vanquish UHPLC columns enables the
resolution of very complex mixtures.
Mobile Phase A: Water

4 Solid Core C18 2.6 m Mobile Phase B: Acetonitrile
8
100 2.1 mm Gradient: Solid core C18 2.6m 100 2.1mm
16 Time (min) %B
1 15
14 0 20
5 6.9 40
6
12.1 80
Accucore Vanquish C18 1.5m 100 2.1mm
7 11 Time (min) %B
2 3 9 17 0 20
10 12
18 4 40
13
7 80
Temperature: 43C
Flow Rate: Solid Core C18 2.6m
mAU
100 2.1mm = 380L/min

Accucore Vanquish C18 1.5m
Accucore Vanquish C18 1.5 m
100 2.1mm = 650L/min
100 2.1 mm Injection Volume: 0.5L
Detection: UV, 230nm (0.1s rise time, 50Hz)
Analytes: 1. Desethylatrazine 10. Diuron
2. Metoxuron 11. Isoproturon
3. Hexazinone 12. Metobromuron

4. Simazine 13. Metazachlor
5. Cyanazine 14. Sebuthylazin
6. Methabenzthiazuron 15. Propazine
7. Chlorotoluron 16. Terbuthylazine
8. Atrazine 17. Linuron
9. Monolinuron 18. Metolachlor
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0
Minutes
Comparison of separation of 18 pesticides using Accucore Vanquish 1.5m column

to a larger particle size solid core column
4-006
More results

High efficiency is maintained even at high flow rates enabling fast reproducible separations.
20
Accucore Vanquish 1.5m
Accucore Vanquish C18,1.5m
18
Solid core 1.7m
Solid Core C18 1.7m
16
Solid core 1.6m
Solid Core C18 1.6m
14 Mobile Phase: Water:acetonitrile (50:50)
12 Temperature: 30C
Flow Rate: 0.1 to 0.6mL/min
H (m)
10
0
0.0 1.0 2.0 3.0 4.0 5.0 6.0
(mm/s)
Reproducibility
The advanced bonding and automated packing technology used in the manufacture of Accucore
Vanquish UHPLC columns results in exceptionally reproducible chromatography.
5.0 Naproxen Fenoprofen Ibuprofen
Accucore
4.5
Accucore Vanquish C18 1.5m
4.0
Mobile Phase: Acetonitrile:20 mM ammonium formate
3.5
Capacity Factor ('k)
pH 3.0 (50:50)
3.0 Temperature: 30C
2.5 Flow Rate: 0.4mL/min
2.0
1.5
1.0
0.5
0.0
0 500 1000 1500 2000 2500 3000 3500 4000
Injection Number
RSD for retention time less than 0.3%

%RSD for 4000
Naproxen Fenoprofen Ibuprofen
injections RSD for peak area less than 0.6%
Retention time 0.19 0.25 0.29 RSD for peak height less than 1.2%
Capacity factor 0.41 0.42 0.41 Column pressure stable over 4000 injections
Efficiency 2.94 2.74 2.80 (RSD 0.53%)
Asymmetry 0.92 0.87 1.09 Pressure 500 bar
Peak area 0.53 0.50 0.55
Peak height 1.13 1.02 0.91
Pressure 0.53
Easier interaction
Accucore Vanquish UHPLC columns, the Vanquish UHPLC System and the Thermo Scientific Dionex
Chromeleon Chromatography Data System are combined into a seamless workflow solution, which
allows for simple and easy to implement separations.
Accucore Vanquish
Particle Size (m) Format Length (mm) ID (mm) C18+
1.5 UHPLC Column 50 2.1 27101-052130
100 2.1 27101-102130
150 2.1 27101-152130
Accucore RP-MS pH Range
Optimized for MS detection 0 2 to 9 14

Hydrophobicity
Excellent peak shapes
Excellent combination of speed and efficiency Low High
Pore Size
Accucore RP-MS uses an optimized alkyl chain length for more effective coverage of 0 80 300
the silica surface. This coverage results in a significant reduction in non-hydrophobic Carbon Load (%)
interactions and thus highly efficient peaks with very low tailing.
0 7 25
RP-MS offers slightly lower retention than C18 and this combined with high efficiencies
Particle Size 2.6m
and low peak tailing make this the phase of choice for use with MS detection.
The selectivity offered by Accucore RP-MS matches that of C18 columns.
Bases
240 Accucore RP-MS 2.6m, 50mm x 2.1mm
4
Mobile Phase: 65% Methanol / 35% 25mM
Potassium Phosphate pH7.0
200 2 Temperature: 30C
Flow Rate: 500L/min
Injection Volume: 1L
160 Backpressure: 232 bar
Detection: UV, 215nm
Analytes: 1. Uracil
1 2. Propranolol
120
mAU
3. Butylparaben
4. Naphthalene
3
5 5. Acenaphthene
80
6 6. Amitriptyline
40
Tf = 1.27
0
0.0 0.5 1.0 1.5 2.0 2.5 3.0
Minutes
Accucore RP-MS
Particle Size (m) Format Length (mm) 2.1mm ID 3.0mm ID 4.6mm ID
2.6 Defender Guard (4/pk) 10 17626-012105 17626-013005 17626-014005
HPLC Column 30 17626-032130 - -
50 17626-052130 17626-053030 17626-054630
100 17626-102130 17626-103030 17626-104630

150 17626-152130 17626-153030 17626-154630
UNIGUARD Drop-in 10 852-00 852-00 850-00
Guard Cartridge Holder
4-008
Accucore C18 pH Range

Optimum retention of non-polar compounds 0 1 to 11 14
Hydrophobicity
Hydrophobic interaction mechanism
Separates a broad range of analytes Low High
Pore Size
The carbon loading of Accucore C18 phase provides high retention of non-polar 0 80 300
analytes via a predominantly hydrophobic interaction mechanism. Carbon Load (%)
The highly retentive nature of Accucore C18 phase means that it can be used 0 9 25
to separate a broad range of analytes.
Particle Size 2.6m USP L1
Triazines
Accucore C18 2.6m, 50mm x 2.1mm
100 Mobile Phase A: Water
Mobile Phase B: Acetonitrile
3
Gradient: Time (min) %B
80 1.0 35
2 2.5 70
4 Temperature: 25C
6
60 Flow Rate: 600L/min
mAU
1 Injection Volume: 2L
Backpressure: 298 bar
40
Accucore
5 Analytes: 1. Simazine
2. Simetryn
3. Atrazine
20 4. Ametryn
5. Propazine
6. Prometryn
0
0.0 0.5 1.0 1.5 2.0 2.5 3.0
Minutes
Accucore C18
HPLC Column 30 17126-032130 - -
50 17126-052130 17126-053030 17126-054630
100 17126-102130 17126-103030 17126-104630
150 17126-152130 17126-153030 17126-154630
4 Drop-in Guard (4/pk) 10 74104-012101 74104-013001 74104-014001
HPLC Column 50 74104-052130 74104-053030 74104-054630
100 74104-102130 74104-103030 74104-104630
150 74104-152130 74104-153030 74104-154630
250 74104-252130 74104-253030 74104-254630
UNIGUARD Drop-in 10 852-00 852-00 850-00
pH Range
2 to 9 14
Accucore C8
0
pH Range
Hydrophobicity
Lower hydrophobic retention 0 2 to 9 14

Low High Hydrophobicity
Complementary steric selectivity to C18
Pore Size
Low levels of secondary interactions Low High
Recommended Pore Size

0 for80 moderately
polar analytes 300
Carbon Load (%)
0 80 300
Accucore C8 HPLC columns offer lower hydrophobic retention than columns packed Carbon Load (%)
0 5 25
with longer alkyl chain length material, such as C18, and are therefore
Particlefor
recommended Size 2.6m with medium hydrophobicity or USP
analytes whenL7a less hydrophobic
0 5 25
phase provides optimum retention. Particle Size 2.6m USP L7
The low levels of secondary interactions demonstrated in the phase characterization

are the result of excellent bonded phase coverage and allow users of Accucore C8
HPLC columns to benefit from excellent peak shapes.
Testosterone Testosterone
Testosterone
100 Accucore C8 2.6m, 50 x 2.1mm

Column: 100Accucore C8 2.6 m, 50 x 2.1 mm Colum
Mobile Phase A: Water + 0.1% formic acid
90 Mobile phase A: B: 90water + 0.1% formic acidformic acid Mobil
Mobile Phase Acetonitrile + 0.1%
80 Gradient:
Mobile phase B: 80 595 % B in 0.8 minutes
acetonitrile + 0.1% formic acid Mobil
Temperature: 70 60C Gradie
Relative Abundance
70 Gradient: 5951500L/min
% B in 0.8 minutes
Relative Abundance
Flow Rate: 60 Flow:

60 Flow:Injection Volume:
15005L
L/min
50
Detection: ESI-MS/MS Tempe
50 Temperature: 60 C
40 Injecti
40 Injection:Retention time5 (tR
30 L /min) Detec
0.73
30 Detection: %RSD20ESI-MS/MS
tR 0.22
20 %RSD 10
Area 3.01
10 0
0.0(t /min)
Retention time 0.2 0.4 0.6 0.73 0.8 1.0 1.2 1.4
0
Data from%RSD
sixt injections.
R
Minutes
0.22
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 R
%RSD Area 3.01

Minutes Data
Data from six injections.
Accucore C8
HPLC Column 30 17226-032130 - -
50 17226-052130 17226-053030 17226-054630
100 17226-102130 17226-103030 17226-104630

150 17226-152130 17226-153030 17226-154630
4 Drop-in Guard (4/pk) 10 74204-012101 74204-013001 74204-014001
HPLC Column 50 74204-052130 74204-053030 74204-054630
100 74204-102130 74204-103030 74204-104630
150 74204-152130 74204-153030 74204-154630
250 74204-252130 74204-253030 74204-254630
UNIGUARD Drop-in
10 852-00 852-00 850-00
Guard Cartridge Holder 22
22
4-010
Accucore aQ pH Range

Retention and resolution of polar analytes 0 2 to 9 14
Hydrophobicity
Polar endcapped C18 stationary phase for alternative selectivity
Ideal for highly aqueous mobile phases Low High
Pore Size
The polar functional group used to endcap Accucore aQ phase provides an 0 80 300
additional controlled interaction mechanism by which polar compounds can be Carbon Load (%)
retained and resolved, making the Accucore aQ phase ideal for the quantitative
0 9 25
analysis of trace levels of polar analytes.
The wettability of reversed phase media can be increased by the introduction of
polar functional groups. The polar endcapping of Accucore aQ media also makes it
usable in 100% aqueous mobile phases without the risk of loss of performance or
poor stability.
Lamivudine (USP)
mn: 14050 x 2.1 mm

Accucore C8 2.6 m, Accucore aQ 2.6m, 50mm x 2.1mm
le phase A: water + 0.1% formic acid Mobile Phase: 95:5 (v/v) Ammonium Acetate,
120 pH 3.80 / Methanol
le phase B: acetonitrile + 0.1% formic acid
Accucore
Temperature: 35C
ent: 100
595 % B in 0.8 minutes Flow Rate: 200L/min
mAU
1500 L/min Injection Volume: 1L

80 Detection: UV, 277nm
erature: 60 C
Analytes: Lamivudine
tion: 5 L 60 Asymmetry 1.36
ction: ESI-MS/MS %RSD t r 0.00
40 %RSD Peak area 1.72
(%RSD calculated from 6 replicate injections)
20 USP acceptance criteria: % RSD (t r, Peak Area) <2.0
Retention time (tR /min) 0.73
%RSD tR 0.22
0
%RSD Area 3.01
0.0 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0
a from six injections. Minutes
Accucore aQ
HPLC Column 30 17326-032130 - -
50 17326-052130 17326-053030 17326-054630
100 17326-102130 17326-103030 17326-104630
150 17326-152130 17326-153030 17326-154630
UNIGUARD Drop-in 10 852-00 852-00 850-00
0 150 300
Carbon Load (%)
0 8 25
Accucore Polar Premium pH Range

Rugged amide-embedded C18 phase 0 1.5 to 10 14

Hydrophobicity
Selectivity complementary to conventional C18 phases
Stable over a wide pH range and compatible with 100% aqueous Low High
mobile phase Pore Size
0 150 300
Accucore Polar Premium is an exceptionally rugged polar embedded reverse phase Carbon Load (%)
material that offers high efficiency, wider operating pH range and unique selectivity
0 8 25
complementary to standard C18 phases.
The specially designed bonded phase is stable from pH 1.5 to 10.5 and will not
undergo phase collapse in 100% aqueous mobile phase.
Curcuminoids (Tumeric)
600
ccucore Polar Premium 2.6m, 100 x 3.0mm
A
1, 2, 3
1,2,3 Fused Core C18, 100 x 3.0mm
Mobile Phase: Methanol : 10mM Phosphoric Acid, 80 : 20
500
Temperature: 40C
Flow Rate: 800L/min
Fused core C18
Analytes: 1. Curcumin
2. Desmethoxycurcumin
300 Fused Core C18
3. Bis-desmethoxycurcumin
mAU
200
1 The Accucore Polar Premium HPLC column provides desirable
selectivity that resolves the major and minor component
100
2 Accucore Polar Premium under simple isocratic conditions
600
in less than three minutes,
3 1, 2, 3
while the C18 columns fail to separate these components.
1,2,3
500
-50
0.00 1.00 2.00 3.00
400
Minutes Fused core C18
300 Fused Core C18

mAU
200
1
Accucore Polar Premium
Particle Size (m) Format Length (mm) 2.1mm ID 100
3.0mm ID 4.6mm ID Accucore Polar Premium
2
3
2.6 Defender Guard (4/pk) 10 28026-012105 - -
HPLC Column 50 28026-052130 28026-053030 28026-054630

-50
100 28026-102130 0.00 28026-1030301.00 28026-104630
2.00 3.00
Minutes
150 28026-152130 28026-153030 28026-154630
250 28026-252130 - -
UNIGUARD Drop-in 10 852-00 852-00 850-00
4-012
Accucore Phenyl-Hexyl pH Range

Mixed-mode selectivity for aromatic and moderately polar analytes 0 2 to 8 14
Hydrophobicity
Enhanced pi-pi interactions with aromatics
Moderate hydrophobicity Low High
Pore Size
The C6 chain in Accucore Phenyl-Hexyl phase exhibits classical RP retention 0 80 300
and selectivity, while the phenyl ring can add special selectivity by interacting Carbon Load (%)
with polar groups within the solutes. This results in a mixed-mode separation
0 5 25
mechanism. The reduced hydrophobicity of this phase makes it ideal for the
separation of very non-polar compounds. Particle Size 2.6m USP L11
The Phenyl-Hexyl phase should be selected for complex samples where some peaks
are well resolved on a conventional alkyl phases, but are not well resolved on a
conventional phenyl phase, or when other peaks are well resolved on a phenyl
phase, but not well resolved on a conventional alkyl phase.
Beta-agonists
Accucore Phenyl-Hexyl 2.6m, 100mm x 2.1mm
100 2.89 NL: 9.22E5
m/z = 225.60-226.60 Mobile Phase A: Ammonium Acetate 5mM, pH 4
Accucore
Terbutaline
0 Gradient: Time (min) %B
100 3.04 NL: 6.51E5 0 5
m/z = 239.60-240.60 MS 1 5
10 100
Cimaterol Temperature: 40C
0
100 NL: 3.52E6 3.16 Flow Rate: 0.25mL/min
m/z = 219.60-220.60 Injection Volume: 1L
Backpressure: 120 bar (at t0)
Salbutamol
0 Detection: +ESI-MS (45C, 4.5kV, 60V,
100 NL: 2.23E6 4.72 scan 150 350)
m/z = 301.60-302.60 MS
Ractopamine
0
100 NL: 2.51E6 4.80
m/z = 213.60-214.60 MS
Clorprenaline
0
100 NL: 1.71E6 5.13
m/z = 276.60-277.60 MS
Clenbuterol
0
0 1 2 3 4 5 6 7
Minutes
Accucore Phenyl-Hexyl
HPLC Column 30 17926-032130 - -
50 17926-052130 17926-053030 17926-054630
100 17926-102130 17926-103030 17926-104630
150 17926-152130 17926-153030 17926-154630
UNIGUARD Drop-in 10 852-00 852-00 850-00
0 80 300
Carbon Load (%)
0 6 25
Particle Size 2.6m

Accucore Phenyl-X pH Range
Unique reversed-phase shape selectivity 0 2 to 8 14

Hydrophobicity
Enhanced selectivity for aromatic compounds
Compatible with highly aqueous mobile phases Low High
Pore Size
Robust, high-efficiency, low column bleed
0 80 300
The proprietary Accucore Phenyl-X alkyl aromatic bonded phase provides a unique Carbon Load (%)
selectivity when compared to other reversed phase materials such as C18 or Phenyl.
0 6 25
The advanced design of the bonded phase makes it compatible with highly aqueous
Particle Size 2.6m
mobile phases and robust, demonstrating very low bleed.
Phenyl-X exhibits particularly high aromatic selectivity.
Estrogens
1
120
ccucore Phenyl-X 2.6m, 100 x 2.1mm
A
Fused Core C18, 100 x 2.1mm
100
Mobile Phase: 15:40:45 (v/v) Acetonitrile:Methanol:Water
2 4 Temperature: 40C
80
3
Flow Rate: 400L/min
mAU
60
20
Fused Core C18
0
0.0 0.5 1.0 2.5 2.0 2.5 3.0 3.5 4.0 5.5 6.0 1. Estriol (E3) 2. Estradiol (E2)
Minutes
OH OH
150 H3C H3 C
1 OH
H
H
125
H H
H H
100 HO
HO
2 3 2.04 1
mAU
75
4 120
3. Estrone (E1) 4. Ethynylestradiol
50 100
OH OH
H3C H3C 2
25 80 CH 4
H H 3
mAU
0 60
H H H H
HO HO 40
0.0 0.5 1.0 2.5 2.0 2.5 3.0 3.5 4.0 5.5 6.0
Minutes 20
Fused Core C18
0
0.0 0.5 1.0 2.5 2.0 2.5 3.0 3.5 4.0 5.5 6.0 1. Estriol (E3)
Minutes
150 1
Accucore Phenyl-X
125
H
Particle Size (m) Format Length (mm) 2.1mm ID 100 3.0mm ID 4.6mm ID HO
2.6 Defender Guard (4/pk) 10 27926-01210575 - 2

- 3 2.04
mAU
4
3. Estrone (E1
HPLC Column 50 27926-05213050 27926-053030 27926-054630
100 27926-10213025 27926-103030 27926-104630
150 27926-1521300 27926-153030 27926-154630
HO
250 27926-252130 0.0 0.5
- 1.0 2.5 2.0 2.5
- 3.0 3.5 4.0 5.5 6.0
Minutes
UNIGUARD Drop-in 10 852-00 852-00 850-00

4-014
Accucore PFP pH Range

Alternative selectivity to C18 0 2 to 8 14
Hydrophobicity
Extra retention for halogenated species
Unique selectivity for non-halogenated polar compounds Low High
Pore Size
The introduction of fluorine groups into the Accucore PFP (pentafluorophenyl) 0 80 300
stationary phase causes significant changes in solute-stationary phase interactions. Carbon Load (%)
This can lead to extra retention and selectivity for positional isomers of
0 5 25
halogenated compounds.
PFP Columns are also well suited to the selective analysis of non-halogenated
compounds, in particular polar compounds containing hydroxyl, carboxyl, nitro,
or other polar groups. High selectivity is often most apparent when the functional
groups are located on an aromatic or other rigid ring system.
Positional isomers
50 Accucore PFP 2.6m, 50mm x 2.1mm
5 Mobile Phase A: 0.1% Formic Acid in Water
Mobile Phase B: 0.1% Formic Acid in Acetonitrile
40 Gradient: 15-30%B in 7 minutes
Temperature: 50C
Accucore
7 Flow Rate: 600L/min
30 1 Detection: UV, 270nm
89 Analytes: 1. 3,4 Dimethoxyphenol
10 2. 2,6 Dimethoxyphenol
mAU
20 2 3. 2,6 Difluorophenol
12
11 4. 3,5 Dimethoxyphenol
13 14 5. 2,4 Difluorophenol
10 34 6 6. 2,3 Difluorophenol
7. 3,4 Difluorophenol
8. 3,5 Dimethylphenol
9. 2,6 Dimethylphenol
0 10. 2,6 Dichlorophenol
11. 4 Chloro-3-Methylphenol
12. 4 Chloro-2-Methylphenol
0 1 2 3 4 5 6 7 8 13. 3,4 Dichlorophenol
Minutes 14. 3,5 Dichlorophenol
Accucore PFP
HPLC Column 30 17426-032130 - -
50 17426-052130 17426-053030 17426-054630
100 17426-102130 17426-103030 17426-104630
150 17426-152130 17426-153030 17426-154630
UNIGUARD Drop-in 10 852-00 852-00 850-00
0 150 300
Carbon Load (%)
0 5 25
Accucore C30
pH Range
Ideal for separation of hydrophobic, long alkyl chain compounds 0 2 to 8 14

Hydrophobicity
High shape selectivity for structurally related isomers
Excellent aqueous-compatibility Low High
Pore Size
Accucore C30 offers high shape selectivity for hydrophobic, long chain, structurally 0 150 300
related isomers, for example carotenoids and steroids. This is a different Carbon Load (%)
form of shape selectivity from that measured in the steric selectivity phase
characterisation test. 0 5 25

It is also an excellent alternative to normal-phase columns for lipid analysis. The
optimized bonding density of the long alkyl chains facilitated by a wider pore diameter
particle result in a phase that is stable even in highly aqueous mobile phases.
Vitamin K isomers
250 Accucore C30 2.6m, 100 x 3.0mm

1 Fused Core C18, 100 x 3.0mm
Mobile Phase: Methanol: 2mM Ammonium Acetate, 98:2
Temperature: 20C
200 2+2 Flow Rate: 650L/min
150 Accucore C30 shows better separation for vitamin K1 isomers
Fused Core C18 than the C18 column.
mAU
100 1
50 2
2
0 250
Accucore C30 1
-50 200 2+2

0.00 1.00 2.00 3.00 4.00 5.00 6.00
Minutes
150
Chromatogram showing the separation of Vitamin K compounds Fused Core C18
Minutes 1-Vitamin K2, 2-Vitamin K1 (trans isomer), 2-Vitamin K1 (cis isomer)
mAU
100 1
Accucore C30 50 2

2
2.6 Defender Guard (4/pk) 10 27826-012105 0 - -

Accucore C30
HPLC Column 50 27826-052130 27826-053030 27826-054630
100 27826-102130 -50 27826-103030 27826-104630
0.00 1.00 2.00 3.00 4.00 5.00 6.00
150 27826-152130 27826-153030 27826-154630
Minutes
250 27826-252130 - -
UNIGUARD Drop-in 10 852-00 852-00 850-00
28
4-016
Accucore HILIC pH Range

Enhanced retention of polar and hydrophilic analytes 0 2 to 8 14
Hydrophobicity
Alternative selectivity to C18 without ion-pair or derivatization
Low High
Pore Size
In HILIC mode the separation occurs through two mechanisms. The primary
mechanism is a partitioning effect due to the enriched water layer around the polar 0 80 300
or charged substrate material. The secondary mechanism involves interaction
between the analyte and the active surface moiety.
Analyte properties that govern retention with HILIC phases are acidity/basicity,
which determines hydrogen bonding, and polarizability which determines dipole-
dipole interactions.
The highly organic mobile phases used with Accucore HILIC phase ensure efficient
desolvation in ESI MS detection, which in turn leads to improved sensitivity.
Catecholamines
2 Accucore HILIC 2.6m, 50mm x 2.1mm

Mobile Phase: 85:15 Acetonitrile:100mM
140 Ammonium Formate, pH 3.2
Temperature: 40C
Accucore
120 Flow Rate: 2mL/min
1
100
Analytes: 1. Catechol
80 2. 5-HIAA
mAU
3
3. DOPAC
60 4. Serotonin
5. L-tyrosine
6. Dopamine
40 7. L-DOPA
5
6
20 7
0
0 0.5 1 1.5 2 2.5 3 3.5 4
Minutes
Accucore HILIC
HPLC Column 30 17526-032130 - -
50 17526-052130 17526-053030 17526-054630
100 17526-102130 17526-103030 17526-104630
150 17526-152130 17526-153030 17526-154630
UNIGUARD Drop-in 10 852-00 852-00 850-00
Low High
Pore Size
0 80 300
Carbon Loading
Accucore Urea-HILIC pH Range
Bonded hydrophilic
Particle Size 2.6m stationary phase 0 2 to 8 14
Hydrophobicity
Unique selectivity compared to other HILIC phases
Low ion exchange activity Low High
Pore Size
Accucore Urea-HILIC has an alternative selectivity and lower ion exchange activity 0 80 300
than other HILIC phases.
Particle Size 2.6m
The bonded hydrophilic stationary phase provides retention of a broad range of
polar analytes using up to 20% aqueous mobile phase.
Analgesic compounds
600 Accucore Urea-HILIC 2.6m, 100 x 2.1mm

2
Mobile Phase: Composition 10:80:10, A : B : C
A: Water
B: Acetonitrile
1
400 C: 100 mM Ammonium Acetate adjusted to pH 4.9
Temperature: 35C
mAU
Flow Rate: 300L/min

3 Injection Volume: 2L into 10L partial loop mode.
Detection: UV, 230nm Analgesic compounds
0
0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1. Acetaminophen 2.600
Salicylic acid 3. Aspirin
2
Minutes tR As tR As Rs tR As Rs
Mean 0.760 1.474 0.908 1.303 2.359 1.100 1.318 3.264
1
CV % 0.00 1.17 0.48 400 0.92 0.49 0.00 0.63 0.48
Data from eight replicate analyses of a mixture of acetaminophen,

mAU
salicylic acid and aspirin

Retention time (tR), peak asymmetry (As200
), peak resolution (Rs) 3
0
0 0.2 0.4 0.6 0.8 1.0 1.2 1.4
Accucore Urea-HILIC Minutes
2.6 Defender Guard (4/pk) 10 27726-012105 - -
HPLC Column 50 27726-052130 27726-053030 27726-054630
100 27726-102130 27726-103030 27726-104630
150 27726-152130 27726-153030 27726-154630

250 27726-252130 - -
UNIGUARD Drop-in 10 852-00 852-00 850-00
4-018
Accucore XL C18

pH Range
Optimum retention of non-polar compounds 0 1 to 11 14

Hydrophobicity
Hydrophobic interaction mechanism
Separates a broad range of analytes Low High
Pore Size
The carbon loading of Accucore XL C18 provides high retention of non-polar 0 80 300
analytes via a predominantly hydrophobic interaction mechanism. Carbon Load (%)
0 7 25
Ibuprofen and Valerophenone (USP) Particle Size 4m USP L1
30 Accucore XL C18
Accucore XL C18 4m, 150 x 4.6mm
25 2 Fully Porous C18 Fully porous C18 5m, 150 x 4.6mm
N = 18274 plates
20 1 S/N = 1202 Mobile Phase: 66.3:33.7 (v/v) Water with
Phosphoric Acid, pH 2.5:Methanol
15 2
1 N = 10538 plates Temperature: 30C
mAU
10 S/N = 534 Flow Rate: 2mL/min

5
0 Analytes: 1. Valerophenone
-5 2. Ibuprofen
0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0
Minutes
Accucore
30
25 2
N = 18274 plates
Accucore XL C8 pH Range
20 1 S/N = 1202
15
mAU
10 0 2 to 9 14
Similar selectivity to C18 with lower retention
5 Hydrophobicity
Recommended
0 for analytes with moderate hydrophobicity
-5 Low High
0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0 Pore Size
Accucore XL C8 is recommended forMinutes
analytes with moderate hydrophobicity,
or when a less hydrophobic phase provides optimum retention. 0 80 300
Carbon Load (%)
0 4 25
Endocrine Disruptors
Particle Size 4m USP L7
400 1
Accucore XL C18
Accucore XL C8 4m, 150 x 4.6mm
300 Fully Porous C18 Fully porous C8 5m, 150 x 4.6mm
3
1 2 Mobile Phase A: Water
Rs = 5.14
200 3 Rs = 4.12 Mobile Phase B: Acetonitrile
mAU
2 5
4 5 Gradient: Time (min) % B
100 4 0.0 25
20.0 70
0 20.1 75
0 1 2 3 4 5 6 7 8 9 10 25.0 25
Minutes Flow rate: 1.5mL/min
Temperature: 25C
Detection: UV at 220nm
400 1 Injection volume: 5L
Analytes 1.Desethyl Atrazine 3.Atrazine
300 2.Simazine 4.Diuron 5.Bisphenol A
3
Accucore
200 XL 2 Rs = 5.14
mAU
5
Particle Size (m) Format 4 Chemistry Length (mm) 2.1mm ID 3.0mm ID 4.6mm ID
100
40 Drop-in Guard (4/pk) C18 10 74104-012101 74104-013001 74104-014001
0 1 2 3 HPLC4 Column5 6 7 8 9 50
10 74104-052130 74104-053030 74104-054630
Minutes
100 74104-102130 74104-103030 74104-104630
150 74104-152130 74104-153030 74104-154630
250 74104-252130 74104-253030 74104-254630
Drop-in Guard (4/pk) C8 10 74204-012101 74204-013001 74204-014001
HPLC Column 50 74204-052130 74204-053030 74204-054630
100 74204-102130 74204-103030 74204-104630
150 74204-152130 74204-153030 74204-154630
250 74204-252130 74204-253030 74204-254630
Hypersil GOLD HPLC

and UHPLC Columns
Outstanding peak shape for your separations

Thermo Scientific Hypersil GOLD columns are an ideal choice for new method
development, delivering outstanding peak shape and increased sensitivity for your
separations.
Solutions for all

Separation Needs
Particle sizes and column designs optimized for all
analyses
Improved Sensitivity
Excellent peak symmetry
Enhanced Resolution
Separation of complex samples
pH Stability
Robust assays at extended pH
The Hypersil GOLD web page contains the latest news, applications
and downloads for the Hypersil GOLD HPLC column range.
Visit www.thermoscientific.com/hypersilgold
4-020
Hypersil GOLD

pH Range
Endcapped, ultra-pure, silica-based columns with exceptional peak 0 1 to 11 14

Hydrophobicity
shape and resolution for HPLC and LC-MS
Low High
Pore Size
Significant reduction in peak tailing while retaining C18 selectivity
Excellent resolution, efficiency and sensitivity 0 175 300
Carbon Load (%)
Confidence in the accuracy and quality of analytical data
0 10 25
Particle Size 1.9m, 3m, 5m, 12m USP L1
Seven +0.1%foric grad 15 to in

H2O / MeCN b-blockers 100%1inminute
1min; 0.5ml/min; 30C; 1.9um Gold 20x2.1mm 550C; 3.5kV; 20V
RT: 0.00 - 1.00 SM: 7G
7 Hypersil GOLD, 1.9m, 20 x 2.1mm
100 NL: 6
Mobile Phase A: H 2O+0.1%formic acid
90 1.02E5
Mobile Phase B: MeCN+0.1%formic acid
Base Peak
MS Gradient: 15 to 100% B in 1min
80
Blockers24 3 Temperature: 30C
Flow Rate: 0.5mL/min
Relative Abundance
70
Detection: +ESI
60 1 Analytes: 1. Atenolol
5
2. Nadolol
50 3. Pindolol
4
Hypersil GOLD
40 4. Timolol
2 5. Metoprolol
30 6. Oxprenolol
7. Propanolol
20
10
0
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
Minutes
Hypersil GOLD
Particle Size (m) Format Length (mm) 1.0mm ID 2.1mm ID 3.0mm ID 4.0mm ID 4.6mm ID
1.9 UHPLC Column 20 - 25002-022130 - - -
30 25002-031030 25002-032130 - - -
50 25002-051030 25002-052130 25002-053030 - 25002-054630
100 25002-101030 25002-102130 25002-103030 - -
150 - 25002-152130 - - -
200 - 25002-202130 - - -
3 Drop-in Guard (4/pk) 10 25003-011001 25003-012101 25003-013001 25003-014001 25003-014001
HPLC Column 30 - 25003-032130 - - 25003-034630
50 - 25003-052130 25003-053030 25003-054030 25003-054630
100 25003-101030 25003-102130 25003-103030 25003-104030 25003-104630
150 25003-151030 25003-152130 25003-153030 25003-154030 25003-154630
5 Drop-in Guard (4/pk) 10 - 25005-012101 25005-013001 25005-014001 25005-014001
HPLC Column 30 - 25005-032130 25005-033030 - 25005-034630
50 - 25005-052130 25005-053030 - 25005-054630
100 - 25005-102130 25005-103030 - 25005-104630
150 - 25005-152130 25005-153030 25005-154030 25005-154630
250 - 25005-252130 25005-253030 25005-254030 25005-254630
UNIGUARD Drop-in 10 851-00 852-00 852-00 850-00 850-00
Guard Cartridge
Holder
Hypersil GOLD Preparative
Particle Size (m) Format Length (mm) 10mm ID 20mm ID 21mm ID 30mm ID 50mm ID
5 Preparative Guard 10 25005-019023 25005-019223 - - -
Cartridge (3/pk)
Preparative HPLC 50 25005-059070 - - 25005-059370 25005-059570
Column
100 25005-109070 - 25005-109270 25005-109370 25005-109570
150 25005-159070 - 25005-159270 25005-159370 25005-159570
250 25005-259070 - 25005-259270 25005-259370 25005-259570
Cartridge (3/pk)
Preparative HPLC 50 25012-059070 - 25012-059270 25012-059370 25012-059570
Column
100 25012-109070 - 25012-109270 25012-109370 25012-109570
150 - - 25012-159270 25012-159370 25012-159570
250 25012-259070 - 25012-259270 25012-259370 25012-259570
Preparative Guard 10 C-1000 854-00 - - -
Cartridge Holder
4-022
Hypersil GOLD C8

pH Range
Recommended for analytes with medium hydrophobicity or when a 0 2 to 9 14

Hydrophobicity
less hydrophobic phase is required to obtain optimum retention
Low High
Pore Size
Similar selectivity to C18 columns but with reduced retention
Lower hydrophobicity, allowing compounds to elute quicker 0 175 300
Carbon Load (%)
Faster separations
Excellent peak shape 0 8 25
High efficiency Particle Size 1.9m, 3m, 5m USP L7
Outstanding sensitivity
b-carotene
600 1
Hypersil GOLD C8 5m, 150 x 4.6mm
Mobile Phase: MeOH
Temperature: 25C
500
400 Analytes: 1. Lutein
2. Lycopene
Hypersil GOLD
3. -Carotene
300
mAU
200
2 3
100
0 1 2 3 4 5 6 7
Minutes
Hypersil GOLD C8
1.9 UHPLC Column 20 - 25202-022130 - - -
30 - 25202-032130 - - -
50 - 25202-052130 25202-053030 - 25202-054630
100 25202-101030 25202-102130 25202-103030 - -
150 - 25202-152130 - - -
3 Drop-in Guard (4/pk) 10 25203-011001 25203-012101 25203-013001 25203-014001 25203-014001
HPLC Column 50 - 25203-052130 25203-053030 25203-054030 25203-054630
100 25203-101030 25203-102130 25203-103030 25203-104030 25203-104630
150 - 25203-152130 25203-153030 25203-154030 25203-154630
5 Drop-in Guard (4/pk) 10 - 25205-012101 25205-013001 25205-014001 25205-014001
HPLC Column 50 - 25205-052130 25205-053030 - 25205-054630
100 - 25205-102130 25205-103030 - 25205-104630
150 - 25205-152130 25205-153030 25205-154030 25205-154630
250 - 25205-252130 25205-253030 25205-254030 25205-254630
UNIGUARD Drop-in 10 851-00 852-00 852-00 850-00 850-00
Hypersil GOLD C4
pH Range
Lower hydrophobicity than C18 or C8 recommended for very 0 2 to 8 14

Hydrophobicity
hydrophobic analytes
Low High
Pore Size
Lower hydrophobicity
Faster separations 0 175 300
Carbon Load (%)
Excellent peak shape
High efficiency 0 5 25
Outstanding sensitivity Particle Size 1.9m, 3m, 5m USP L26
Fatty acids
200 Hypersil GOLD C4 1.9m, 100 x 2.1mm

1
Mobile Phase: H 2O / MeCN (20:80)
Temperature: 30C
160
Detection: 200 nm
120 2 Analytes: 1. Linolenic acid
mAU
2. Linoleic acid
80
40
0
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0
Minutes
Hypersil GOLD C4
Particle Size (m) Format Length (mm) 1.0mm ID 2.1mm ID 3.0mm ID 4.6mm ID
1.9 UHPLC Column 50 - 25502-052130 - -
100 - 25502-102130 - -
150 - 25502-152130 - -
3 Drop-in Guard (4/pk) 10 25503-011001 25503-012101 25503-013001 25503-014001

HPLC Column 50 - 25503-052130 - -
100 - 25503-102130 25503-103030 25503-104630
150 25503-151030 25503-152130 25503-153030 25503-154630
5 Drop-in Guard (4/pk) 10 - 25505-012101 25505-013001 25505-014001
HPLC Column 50 - 25505-052130 - 25505-054630
100 - 25505-102130 25505-103030 25505-104630
150 - 25505-152130 - 25505-154630
250 - 25505-252130 25505-253030 25505-254630
UNIGUARD Drop-in 10 851-00 852-00 852-00 850-00
4-024
Hypersil GOLD aQ

pH Range
Controlled interaction mechanism by which polar analytes can 0 2 to 9 14

Hydrophobicity
be retained and resolved
Low High
Pore Size
Polar endcapped C18 phase for alternative selectivity
Retention and resolution of polar analytes 0 175 300
Carbon Load (%)
Stable in 100% aqueous mobile phases
0 12 25
Particle Size 1.9m, 3m, 5m USP L1

Water soluble vitamins
1200 3
2 Hypersil GOLD aQ 5m, 150 x 4.6mm
6 8 Mobile Phase A: 50 mM KH 2 PO 4, pH 3.5
1000 Mobile Phase B: MeOH
Gradient: 0 100% B in 15 min
800 Flow Rate: 1mL/min
600 Analytes: 1. Vitamin B1 (thiamine)
2. Vitamin B6 (pyridoxine)
mAU
3. Vitamin B3 (nicotinamide)
400
4. Vitamin B5 (pantothenic acid)
5. Folic Acid
-
4
200 7 6. Vitamin B12 (cyanocobalamin)
Hypersil GOLD
5 7. Vitamin H (biotin)
0 8. Vitamin B2 (riboflavin)
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
Minutes
Hypersil GOLD aQ
1.9 UHPLC Column 20 - 25302-022130 - - -
30 - 25302-032130 - - -
50 25302-051030 25302-052130 25302-053030 - 25302-054630
100 25302-101030 25302-102130 25302-103030 - -
150 - 25302-152130 - - -
200 - 25302-202130 - - -
3 Drop-in Guard (4/pk) 10 25303-011001 25303-012101 25303-013001 25303-014001 25303-014001
HPLC Column 30 - 25303-032130 25303-033030 - -
50 - 25303-052130 25303-053030 25303-054030 25303-054630
100 25303-101030 25303-102130 25303-103030 25303-104030 25303-104630
150 25303-151030 25303-152130 25303-153030 25303-154030 25303-154630
5 Drop-in Guard (4/pk) 10 - 25305-012101 25305-013001 25305-014001 25305-014001
HPLC Column 50 - 25305-052130 25305-053030 - 25305-054630
100 - 25305-102130 25305-103030 - 25305-104630
150 - 25305-152130 25305-153030 - 25305-154630
250 - 25305-252130 - 25305-254030 25305-254630
UNIGUARD Drop-in 10 851-00 852-00 852-00 850-00 850-00
Hypersil GOLD aQ Preparative

Cartridge (3/pk)
Column
100 25305-109070 - 25305-109270 25305-109370 25305-109570
150 25305-159070 - 25305-159270 25305-159370 25305-159570
250 25305-259070 - 25305-259270 25305-259370 25305-259570
Hypersil GOLD PFP
pH Range
Introduction of a fluorine group into the stationary phase causes 0 2 to 8 14

significant changes in solute-stationary phase interaction Hydrophobicity
Low High
Pore Size
Fluorine atoms around the phenyl ring enhance pi-pi interactions
with aromatic molecules 0 175 300
Extra retention for halogenated species Carbon Load (%)
Selectivity for non-halogenated polar compounds

0 8 25

Polyphenols
Hypersil GOLD PFP 1.9m, 50 x 2.1mm

70000 2
Mobile Phase: 0.1% Acetic Acid
60000
Temperature: 25C
50000 Injection Volume: 0.5L
40000 3 Analytes: 1. Pyrogallol
uAU
2. Hydroquinone
30000 3. Resorcinol
4. Phenol
20000
4
1
10000
0
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5
Minutes
Hypersil GOLD PFP

1.9 UHPLC Column 20 - 25402-022130 - - -
30 25402-032130 - - -
50 25402-051030 25402-052130 25402-053030 - 25402-054630
100 25402-101030 25402-102130 25402-103030 - -
150 - 25402-152130 - - -
200 - 25402-202130 - - -
3 Drop-in Guard (4/pk) 10 25403-011001 25403-012101 25403-013001 25403-014001 25403-014001
HPLC Column 30 - 25403-032130 25403-033030 - 25403-034630
50 - 25403-052130 25403-053030 25403-054030 25403-054630
100 25403-101030 25403-102130 25403-103030 25403-104030 25403-104630
150 25403-151030 25403-152130 25403-153030 - 25403-154630

5 Drop-in Guard (4/pk) 10 - 25405-012101 25405-013001 25405-014001 25405-014001
HPLC Column 50 - 25405-052130 25405-053030 - -
100 - 25405-102130 25405-103030 - 25405-104630
150 - 25405-152130 25405-153030 25405-154030 25405-154630
250 - 25405-252130 - 25405-254030 25405-254630
UNIGUARD Drop-in 10 851-00 852-00 852-00 850-00 850-00
Hypersil GOLD PFP Preparative

Cartridge (3/pk)
Column
100 25405-109070 - 25405-109270 25405-109370 25405-109570
150 25405-159070 - 25405-159270 25405-159370 25405-159570
250 25405-259070 - 25405-259270 25405-259370 25405-259570
4-026
Hypersil GOLD Phenyl

pH Range
Contains a C4 linker which allows for superior alignment of the 0 2 to 8 14

Hydrophobicity
phenyl ring with aromatic molecules
Low High
Pore Size
Enhanced pi-pi interactions with aromatics
Moderate hydrophobicity 0 175 300
Carbon Load (%)
Outstanding peak shape and sensitivity
0 8 25
Antidepressants
100 Hypersil GOLD Phenyl 1.9m, 50 x 2.1mm

1 Mobile Phase A: 0.1% Formic acid
80 3 Mobile Phase B:: 0.1% Formic acid in MeCN
Gradient: 10 60% B in 3.4mins,
5 60 - 90% B in 0.24 min
60 Temperature: 60C
6
mAU
2 Flow Rate: 0.5mL/min

Injection Volume: 0.7L
40
Detection: UV, 225 and 254nm
7 8 Analytes: 1. Uracil
Hypersil GOLD
20 4 2. Acetaminophen
imp 3. p-Hydroxybenzoic acid
4. o-Hydroxybenzoic acid
0 5. Oxazepam
6. Diazepam
0.0 1.0 2.0 3.0 4.0
7. Di-isopropyl phthalate
Minutes
8. Di-n-propyl phthalate
Hypersil GOLD Phenyl

1.9 UHPLC Column 50 - 25902-052130 - - -
100 - 25902-102130 - - -
150 - 25902-152130 - - -
200 - 25902-202130 - - -
3 Drop-in Guard (4/pk) 10 25903-011001 25903-012101 25903-013001 25903-014001 25903-014001
HPLC Column 50 - 25903-052130 - - -
100 - 25903-102130 25903-103030 - 25903-104630
150 25903-151030 - 25903-153030 - 25903-154630
5 Drop-in Guard (4/pk) 10 - 25905-012101 25905-013001 25905-014001 25905-014001
HPLC Column 50 - 25905-052130 - - 25905-054630
100 - 25905-102130 25905-103030 - -
150 - - - - 25905-154630
250 - - 25905-253030 25905-254030 25905-254630
UNIGUARD Drop-in 10 851-00 852-00 852-00 850-00 850-00
Hypersil GOLD CN
pH Range
For both normal phase and reversed-phase separations 0 2 to 8 14

Hydrophobicity
Low High
Provide alternative selectivity with lower hydrophobicity
Pore Size
Excellent peak shape
0 175 300
Outstanding sensitivity
Carbon Load (%)
Less retention for faster analysis
0 4 25
Organic acids
5 Hypersil GOLD CN 5m, 150 x 4.6mm

70 Mobile Phase A: 25 mM KH 2 PO 4 pH2
Mobile Phase B: MeOH
Temperature: 25C
1
3 Analytes: 1. 4-Fluorobenzoic
mVolts
60 2. o-Toluic Acid
2 3. p-Toluic Acid
4. 2,4,6-Trimethylbenzoic Acid
55 5. 2,5-Dimethylbenzoic Acid
50
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5

Minutes
Hypersil GOLD CN
1.9 UHPLC Column 50 - 25802-052130 - - -
100 - 25802-102130 - - -
200 - 25802-202130 - - -
3 Drop-in Guard (4/pk) 10 25803-011001 25803-012101 25803-013001 25803-014001 25803-014001
HPLC Column 30 - 25803-032130 - - -
50 - 25803-052130 - - -
100 - 25803-102130 25803-103030 - 25803-104630

150 25803-151030 25803-152130 25803-153030 - 25803-154630
5 Drop-in Guard (4/pk) 10 - 25805-012101 25805-013001 25805-014001 25805-014001
HPLC Column 50 - 25805-052130 - - 25805-054630
100 - 25805-102130 25805-103030 - -
150 - 25805-152130 - - 25805-154630
250 - 25805-252130 25805-253030 25805-254030 25805-254630
UNIGUARD Drop-in 10 851-00 852-00 852-00 850-00 850-00
4-028
Hypersil GOLD Amino

pH Range
A high performance aminopropyl phase that gives excellent 0 2 to 8 14

Hydrophobicity
chromatographic properties in three modes: weak anion exchange,
reversed-phase and normal phase Low High
Pore Size
Retains anions and organic acids in weak anion exchange 0 175 300
Carbon Load (%)
Excellent for carbohydrate analysis in reversed-phase
Alternative selectivity to silica columns in normal phase chromatography 0 2 25
Outstanding peak shape and sensitivity Particle Size 1.9m, 3m, 5m USP L8
Sugars
14 Hypersil GOLD Amino 5m, 150 x 4.6mm

1 Mobile Phase: MeCN/Water (80:20)
12 Temperature: 35C
Detection: RI
8 Analytes: 1. Fructose
Hypersil GOLD
uRIU
3 2. Glucose
6
2 3. Sucrose
4 4. Maltose
4
5. Lactose
2
5
0
0 2 4 6 8 10 12 14 16 18 20
Minutes
Hypersil GOLD Amino

1.9 UHPLC Column 50 - 25702-052130 - - -
100 - 25702-102130 - - -
150 - 25702-152130 - - -
200 - 25702-202130 - - -
3 Drop-in Guard (4/pk) 10 25703-011001 25703-012101 25703-013001 25703-014001 25703-014001
HPLC Column 30 - 25703-032130 - - -
50 - 25703-052130 - - -
100 - 25703-102130 25703-103030 - 25703-104630
150 25703-151030 25703-152130 25703-153030 - 25703-154630
5 Drop-in Guard (4/pk) 10 - 25705-012101 25705-013001 25705-014001 25705-014001
HPLC Column 50 - 25705-052130 - - 25705-054630
100 - 25705-102130 25705-103030 - -
150 - - - - 25705-154630
250 - 25705-252130 25705-253030 25705-254030 25705-254630
UNIGUARD Drop-in 10 851-00 852-00 852-00 850-00 850-00
Hypersil GOLD AX
pH Range
A novel polymeric amine ligand bonded to highly pure base 0 2 to 8 14

Hydrophobicity
deactivated silica
Low High
Pore Size
Weak anion exchange phase for multiple charged species
Suitable for HILIC retention and separation of highly polar molecules 0 175 300
Carbon Load (%)
Higher efficiency than polymer based ion exchange columns
Outstanding peak shape and selectivity 0 6 25
Particle Size 1.9m, 3m, 5m
Vitamin C
100 Hypersil GOLD AX 5m, 100 x 4.6mm

Mobile Phase: 100 mM Ammonium Acetate pH 6.8/
80 MeCN (30:70)
Temperature: 30C
60
mAU

40
20
0 5 10 15 20 25
Minutes
Hypersil GOLD AX
1.9 UHPLC Column 50 - 26102-052130 - - -
100 - 26102-102130 - - -
150 - 26102-152130 - - -
200 - 26102-202130 - - -
3 Drop-in Guard (4/pk) 10 26103-011001 26103-012101 26103-013001 26103-014001 26103-014001
HPLC Column 30 - 26103-032130 - - -
50 - 26103-052130 - - -
100 - 26103-102130 - - 26103-104630
150 26103-151030 26103-152130 26103-153030 - 26103-154630
5 Drop-in Guard (4/pk) 10 - 26105-012101 26105-013001 26105-014001 26105-014001
HPLC Column 50 - - - - 26105-054630
100 - 26105-102130 - - 26105-104630
150 - 26105-152130 - - 26105-154630
250 - 26105-252130 26105-253030 26105-254030 -
UNIGUARD Drop-in 10 851-00 852-00 852-00 850-00 850-00
4-030
Hypersil GOLD SAX

pH Range
A highly stable quaternary amine strong anion exchange column for 0 2 to 8 14

Hydrophobicity
aqueous and low pH mobile phases
Low High
Pore Size
High stability to aqueous and low pH mobile phases
Ideally suited to the analysis of smaller organic molecules including 0 175 300
Carbon Load (%)
nucleotides and organic acids
Outstanding peak shape and sensitivity 0 2.5 25
Monophosphates
200 Hypersil GOLD SAX 5m, 150 x 4.6mm

Mobile Phase: Aqueous KH 2 PO 4 (50 mM, pH 3)
175
Temperature: 40C
100
Hypersil GOLD
75
mAU
50
25
0 1 2 3 4 5 6 7 8
Minutes
Hypersil GOLD SAX

1.9 UHPLC Column 100 26302-102130 - - -
150 26302-152130 - - -
3 Drop-in Guard (4/pk) 10 26303-012101 26303-013001 26303-014001 26303-014001
HPLC Column 50 26303-052130 - - -
100 26303-102130 26303-103030 - 26303-104630
150 - 26303-153030 - 26303-154630
5 Drop-in Guard (4/pk) 10 26305-012101 26305-013001 26305-014001 26305-014001
HPLC Column 50 26305-052130 - - -
100 26305-102130 26305-103030 - 26305-104630
150 26305-152130 - - 26305-154630
250 26305-252130 26305-253030 26305-254030 26305-254630
UNIGUARD Drop-in 10 852-00 852-00 850-00 850-00
Hypersil GOLD Silica
pH Range
Unbonded, highly pure base deactivated silica media that is the 0 2 to 8 14

Hydrophobicity
backbone of the Hypersil GOLD range of columns
Low High
Pore Size
Highly pure base deactivated silica media
Outstanding peak shape and sensitivity 0 175 300
Carbon Load (%)
0 25
Steroids Particle Size 1.9m, 3m, 5m USP L3
90
Hypersil GOLD Silica 5m, 150 x 4.6mm
80 Mobile Phase: 19:1 (v/v) n-C6H14/EtOH
Temperature: 30C
70
60 Injection volume: 5L
50 Analytes: 1. Progesterone
2. 21-Hydroxyprogesterone-21-acetate
mVolts
40
3. 17-a-Hydroxyprogesterone
30 4. Cortisone
5. 11-a-Hydroxyprogesterone
20 6. Corticosterone
7. Hydrocortisone
10
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Minutes
Hypersil GOLD Silica

1.9 UHPLC Column 50 - 25102-052130 - - -
100 - 25102-102130 - - -
150 - 25102-152130 - - -
200 - 25102-202130 - - -
3 Drop-in Guard (4/pk) 10 25103-011001 25103-012101 25103-013001 25103-014001 25103-014001
HPLC Column 30 - 25103-032130 - - 25103-034630
50 - 25103-052130 - - -
100 - 25103-102130 25103-103030 - -
150 25103-151030 25103-152130 25103-153030 - 25103-154630
5 Drop-in Guard (4/pk) 10 - 25105-012101 25105-013001 25105-014001 25105-014001
HPLC Column 50 - 25105-052130 - - -
100 - 25105-102130 25105-103030 - 25105-104630
150 - 25105-152130 - - 25105-154630
250 - 25105-252130 - 25105-254030 25105-254630
UNIGUARD Drop-in 10 851-00 852-00 852-00 850-00 850-00
4-032
Hypersil GOLD HILIC

pH Range
Retains and separates polar analytes that are problematic 0 2 to 8 14

Hydrophobicity
using reversed-phase columns
Low High
Pore Size
Alternative selectivity to C18
Improved sensitivity for MS detection 0 175 300
Carbon Load (%)
Alternative to ion-pair or derivatization
Outstanding peak shape and selectivity 0 6 25
Urea
100 Hypersil GOLD HILIC 5m, 150 x 4.6mm
Mobile Phase: H 2 O/MeCN (10:90)
+ 0.1% formic acid
80
Temperature: 30C
Relative Abundance
Detection: +ESI
Analytes: 1. Urea
40
Hypersil GOLD
20
0
0 1 2 3 4 5 6 7 8 9 10
Minutes
Hypersil GOLD HILIC

1.9 UHPLC Column 50 - 26502-052130 - - -
100 - 26502-102130 - - -
150 - 26502-152130 - - -
3 Drop-in Guard (4/pk) 10 26503-011001 26503-012101 26503-013001 26503-014001 26503-014001
HPLC Column 30 - 26503-032130 - - -
50 - 26503-052130 - - -
100 - 26503-102130 26503-103030 - 26503-104630
150 26503-151030 26503-152130 26503-153030 - 26503-154630
5 Drop-in Guard (4/pk) 10 - 26505-012101 26505-013001 26505-014001 26505-014001
HPLC Column 50 - 26505-052130 - - 26505-054630
100 - 26505-102130 26505-103030 - -
150 - - - - 26505-154630
250 - 26505-252130 26505-253030 26505-254030 26505-254630
UNIGUARD Drop-in 10 851-00 852-00 852-00 850-00 850-00
Acclaim HPLC
and UHPLC Columns
Optimal selectivity through innovative
chemistries
Designed for separating a variety of analytes, from small neutral and polar
molecules to complex mixtures. Ideal for pharmaceutical, environmental,
food and beverage and chemical applications.
Diversified Selectivities
Novel and proprietary surface chemistries
O OH
Reproducible and Reliable
Strict manufacturing and quality processes O
High Efficiencies O
For optimum resolution of complex mixtures
Ultra-pure, Porous,
Spherical Silica
Providing consistent quality and performance
The Acclaim web page contains the latest news, applications

and downloads for the Acclaim HPLC column range.
Visit www.thermoscientific.com/acclaim
4-034
Acclaim 120 C18

pH Range
High performance reversed-phase columns for reproducible results 0 2 to 8 14

Hydrophobicity
Low High
High hydrophobic retention
Pore Size
Excellent efficiencies for maximum resolution
0 120 300
Low silanol activity for excellent peak shapes for basic analytes
Carbon Load (%)
Extremely low bleed, fully compatible with MS
0 18 25
The Acclaim 120 columns are designed for high resolution reversed-phase separations.
The very high surface coverage and very low metal content together result in columns
with excellent efficiencies. These columns provide exceptional performance for
a variety of applications in the pharmaceutical, chemical, environmental and food
separations areas. A: Acclaim 120 C18, 5m, 150 x 4.6mm
B, C: Acclaim RSLC C18, 2.2m, 50 x 2.1mm
Mobile Phase: 200mM HOAc
in 10% (v/v) MeOH
160 250 220 Column:
Temperature: A: Acclaim 120 C18, 5m,
20C Detector: UV
4 Flow Rate: A: 1.00mL/min
A 4 B 4 C

4.6150
B: 0.41mL/min
mm A: 1
B,C: C: Acclaim RSLC C18, 2m, B:
Acclaim
0.82mL/min
Injection Volume: 2.150
A: 10L mm C: 1
B: 1.2L
Mobile

Phase: 200 mM HOAc
C: 1.2L Peaks: 1. p
mAU
3 Detection: inUV,10% (v/v) MeOH

254 nm, 2. p
3
2
Standard
1 2 RSLC Column 3 Double Velocity Flow:
A:A: 1.00 mL/min
1 Hz data rate
3. V
1 Column 6 Acceleration 2 12 Acceleration B: 5 Hz data rate
1
B:C: 0.41 mL/min
10 Hz data rate 4. V
Analytes: C:1. p-Hydroxybenzoic
0.82 mL/min acid
0 Sample: Com
0 Temperature:
20 C
2. p-Hydroxybenzaldehyde
0 3. Vanillic acid 40%
Injection:
A: 10 L
4. Vanillin
0 10 20 30 40 50 60 0 2 4 6 8 10 0 1 2 3 4 5 Sample: B:Commercial
1.2 L vanilla extract in Reference: AO
Minutes C:40%1.2ethanol,
L filtered
Reference: AOAC Official Method 990.25
Acclaim 120 C18

2.2 RSLC Column 30 071402 071609 -
50 068989 071608 -
75 - 075699 -
100 068990 071607 -
150 071401 - -
250 074814 - -
3 HPLC Column 33 - 066276 -
50 077999 068973 063189
75 - 066277 -
100 077998 078000 078001
mAU
150 063187 063705 063191

250 077997 070080 -
5 Guard Cartridge 10 069692 071985 069699
HPLC Column 150 - - 063197
0 250 - - 063199
0.0 See page 4-037 for Acclaim Guard Holder Ordering Guide
Acclaim 120 C8
pH Range
High performance reversed-phase columns with intermediate 0 2 to 8 14

Hydrophobicity
hydrophobic retention
Low High
Pore Size
Low silanol activity for excellent peak shapes for basic analytes
Excellent column efficiencies 0 120 300
Carbon Load (%)
LC-MS compatible
0 11 25
Acclaim 120 C8 reversed-phase columns feature densely bonded monolayer C8 ligands Particle Size 2.2m, 3m, 5m USP L7
on a high-purity, spherical porous silica substrate. The columns are a well-characterized

line of LC-MS compatible C8 phases with very high surface coverage and extremely
low silanol activity. These columns provide exceptional performance for a variety
of applications in the pharmaceutical, environmental, food and many other industrial
sectors. Triclosan Separation in Toothpaste Using an Acclaim RSLC C8Column
Triclosan in toothpaste
Column: Acclaim RSLC C8, 2.2m, 50 x 2.1mm
80 Mobile Phase: Isocratic,Acclaim
15% buffer, (2mM
2 Column:
RSLC C8, 2.2 m
Ammonium acetate pH5),
Dimension: 2.1 100 mm
85% methanol (v/v)
HPLC System:
Temperature: 50C UltiMate 3000 RSLC
281.1 Buffer:
Flow Rate: 0.2mL/min 2 mM Ammonium acetate pH 5
Mobile Phase:
Injection Volume: 1.0L Isocratic, 15% buffer, 85% methanol (v/v)
200 nm 400 Flow Rate:
Detection: 0.200
Diode array mL/min
detector, 281nm,
1 Volume: 10Hz, 0.11.0
s resp.
Inj. L time and spectra
mAU

Temperature: 20040050 nmC
Analytes: 1. Saccharin
Detection:

Diode array detector, 281 nm, 10 Hz,
2. Triclosan
Sample: Toothpaste0.1containing
s resp. time
0.3%and spectra 200400 nm
triclosan
Samples: Toothpaste containing 0.3% triclosan
Preparation: 1.0 g Toothpaste + 1.0 mL of 7.5 mg/mL
magnesium sulfate + methanol to make
25 mL. Sonicate and filter.
0 Peaks:
0.0 1.0 2.0 3.0 1. Saccharin
Minutes 2. Triclosan
Acclaim 120 C8
2.2 RSLC Column 30 - 072618 -
50 072615 072619 -
100 072616 072620 -
150 072617 - -
250 074811 - -
3 HPLC Column 50 059122 - 059125
100 059123 - 059126
150 059124 068970 059127
250 076185 - -
5 Guard Cartridge 10 069688 071979 069696
HPLC Column 50 059134 - 059138
100 - - 059139
150 059136 - 059140
250 - - 059141
See page 4-037 for Acclaim Guard Holder Ordering Guide
4-036
Acclaim Phenyl-1

pH Range
A unique reversed-phase column with high aromatic selectivity 0 2 to 8 14

Hydrophobicity
Low High
High aromatic selectivity
Pore Size
High hydrophobic retention
0 120 300
Unique and complementary selectivity compared to any other phenyl
Carbon Load (%)
type column
Compatibility with highly aqueous mobile phase 0 13 25
High efficiency and rugged packing Particle Size 3m, 5m USP L11
Acclaim Phenyl-1 has a higher pi-pi interaction than other phenyl phases and provides
unique selectivity for aromatic compounds while maintaining sufficient hydrophobic
interaction and aqueous compatibility for superior chromatographic performance.
The Acclaim Phenyl-1 column can be used in a wide range of applications in
pharmaceutical, environmental, food testing and product-quality testing. This column is
ideally suited for the analysis of aromatic analytes; some examples include
glucocorticosteroids, estrogens, fat-soluble vitamins and phospholipids.
Acclaim
Separation of fat-soluble vitamins Acclaim Phenyl-1, 3m, 150 x 3.0mm
Mobile Phase: Methanol/water v/v 90/10
60
Temperature: 30C
3
Analytes: (100 ppm each)
1. Retinol acetate (vitamin A acetate)
5 2. Vitamin D2
4
mAU
3. Vitamin D3
6 9 4. delta-Tocopherol
8
5. gamma-Tocopherol
6. alpha-Tocopherol (vitamin E)
1 10 7. Impurity (unknown)
7
8. Vitamin E acetate
9. Vitamin K2
10. Vitamin K1
0
0 6 12 18 24 30
Minutes
Acclaim Phenyl-1
3 Guard Cartridge 10 079934 071974 071973
HPLC Column 150 071971 071970 071969
5 HPLC Column 150 - - 088016
250 - - 079697
Acclaim Guard Holder

Format Cat. No.
Acclaim Guard Cartridge Holder V-2 069580
Acclaim Guard Kit (Holder and Coupler) V-2 069707
Guard to Analytical Column Coupler V-2 074188
Acclaim C30 pH Range
Columns for separating structurally related isomers 0

Hydrophobicity
2 to 8 14
Low High
High shape selectivity Pore Size
Unique selectivity complementary to other reversed-phase columns
0 200 300
Compatibility with highly aqueous mobile phase Carbon Load (%)
High-quality: low column bleed, high efficiency and rugged packing
0 13 25
Particle Size 3m, 5m USP L62

The Acclaim C30 is designed to provide high shape selectivity for separating hydrophobic
structural related isomers and unique selectivity complementary to other reversed-phase
columns (e.g. C18).
Column: Acclaim C30, 5 m (4.6 150 mm)

LC System: UltiMate 3000 RS, Dual Gradient
Omega fatty acids Mobile Phases: A. Water:formic acid:mobile phase B
900:3.6:100 (v/v)
6000 LLA B. Acetone:acetonitrile:THF:formic acid
Acclaim C30, 5m, 150 x 4.6mm
675:225:100:4(v/v)
Mobile Phase A: Water:formic acid:mobile phase B
Gradient: Time (min) %A %B
ng
900:3.6:100 (v/v)
100 0 100
Mobile Phase B: Acetone:acetonitrile:THF:formic 0 acid
0 14
675:225:100:4(v/v) 1 40 60
0 9 11
Area 13 Gradient: 13 (min) %A 30 %B 70
Time
8 10 0 22 100 0 5 95
12 1 40 60
2 9 24 5 95
34 13 30 70
1 6 7 22 29 100
5 95 0
A 24 32 100
5 95 0
pA
5
Flow Rate:
1.00
29 mL/min100 0

Temperature: 3032 C 100 0
Temperature:
Inj. Volume: 2 30CL
Detection: Corona ultra, nebulizer 15 C, filter high
B Samples:
Detection:
A.Corona
Standards in isopropanol
ultra, nebulizer 15C, filter high
Analytes: B.1.Saponified
SDA chicken fat
0 Peaks: 1.2.SDA
EPA 8. DPA
10 12 14 16 18 20 22 24 3. ALA
Minutes
2.4.EPA
GLA
9. 9E, 14Z-CLA
3.5.ALA
DHA 10. ETA
4.6.GLA
Arach. 11. Adrenic
5.7.DHA
LLA 12. Oleic
Samples: A. Standards
6. Arach. in
13.isopropanol
EDA
B. Saponified chicken fat
7. LLA 14. Erucic
Acclaim C30

3 HPLC Column 50 078666 - 078661
100 078665 - -
150 075725 075724 075723
250 078664 075726 -
5 Guard Cartridge 10 075722 075721 075720
HPLC Column 150 - - 075719
250 - - 075718

Format Cat. No.
4-038
Acclaim PolarAdvantage pH Range

Novel polar-embedded reversed-phase columns with unique selectivity 0
Hydrophobicity
2 to 8 14
Low High
Selectivity complementary to the C18 column Pore Size
Low silanol activity for excellent peak shape with basic compounds
0 120 300
Compatible with 100% aqueous mobile phase Carbon Load (%)
High selectivity for hydrophobic aromatic molecules
0 16 25
Wide range of applications
Acclaim PolarAdvantage (PA) columns feature a patented bonding column chemistry that incorporates
a polar sulfonamide group with an ether linkage near the silica surface. This unique chemistry provides
low silanol activity, compatibility with 100% aqueous mobile phase. The Acclaim PA column offers great
separation power to resolve a wide variety of polar and non-polar analytes and supports LC-MS analysis.
EPA 604 phenols

Acclaim RSLC PolarAdvantage, 2.2m, 50 x 3.0mm
60 Mobile Phase A: 10mM formic acid + 10mM
ammonium formate, pH 3.75 0.05
Acclaim
7 Mobile Phase B: Acetonitrile
Gradient: -1.5 0.0 0.3 2.6 3.0
%A 70 70 70 10 10
%B 30 30 30 90 90
5 Temperature: 30C
3 Injection Volume: 0.5L
2
mAU
Detection: UV, 280nm, 10Hz, 0.5s resp. time

Analytes: 1. Phenol
6 2. 2,4-Dinitrophenol
4 3. 4-Nitrophenol
1 8 9
4. 2-Chlorophenol
10 5. 2-Nitrophenol
6. 2,4-Dimethylphenol
11 7. 4,6-Dinitro-2-methylphenol
8. 4-Chloro-3-methylphenol
0 9. 2,4-Dichlorophenol
0 0.5 1.0 1.5 2.0 2.5 3.0 10. 2,4,6-Trichlorophenol
11. Pentachlorophenol
Minutes
Sample: Calibration mix, 50g/mL in water
Acclaim PolarAdvantage
2.2 RSLC Column 50 072622 - -
75 - 075698 -
100 072623 072627 -
150 072624 - -
250 074813 - -
3 HPLC Column 50 063174 068972 -
100 061316 076214 -
150 061317 063693 061318
250 - 070079 -
5 Guard Cartridge 10 069691 071983 069698
HPLC Column 50 - - 061319
150 - - 061320
250 - - 061321
Acclaim PolarAdvantage II pH Range
Complementary selectivity and enhanced hydrolytic stability 0

Hydrophobicity
1.5 10 14
Low High
Unique selectivity complementary to the C18 column Pore Size
Novel polar-embedded column chemistry for hydrolytic stability
0 120 300
Compatible with 100% aqueous mobile phase Carbon Load (%)
Low bleed for MS compatibility
0 16 25
Wide range of applications
Acclaim PolarAdvantage II (PA2) columns feature a patented surface chemistry that incorporates
an amide-embedded polar group and multi-point attachment between the ligands and the silica surface.
This unique chemistry provides enhanced hydrolytic stability from pH 1.5-10 with 100% aqueous mobile
phases and exhibits high reversed-phase capacity, with selectivity complementary to conventional
C18 columns.
The Acclaim PA2 column is specifically designed to withstand high pH conditions, making it a good choice
for the separation of both basic and acidic analytes.
Turmeric
200 1, 2, 3 Acclaim RSLC 120 C18 2.2m, 100 x 2.1mm
Acclaim RSLC PA2, 2.2m, 100 x 2.1mm
Mobile Phase A: 15mM H 3PO 4
Mobile Phase B: Methanol
Area: 9.7 mAU*Min. Isocratic: C18: 70% B (v/v)
C18 PA2: 80% B (v/v)
mAU
1 Temperature: 30C
Total area: 9.9 mAU*Min. Detection: UV, 428nm
2 3 Analytes: 1. Curcumin
PA2 2. Demethoxycurcumin
0 3. Bis-demethoxycurcumin
Sample: Turmeric extract
0 1 2 3 4
Minutes
Acclaim PolarAdvantage II
2.2 RSLC Column 30 071402 071609 -

50 068989 071608 -
100 068990 071607 -
150 071401 - -
250 074814 - -
3 HPLC Column 33 - 066276 -
50 077999 068973 063189
75 - 066277 -
100 077998 078000 078001
150 063187 063705 063191
250 077997 070080 -
5 Guard Cartridge 10 069692 071985 069699
HPLC Column 150 - - 063197
250 - - 063199
4-040
Acclaim HILIC-10 pH Range

Designed with unique selectivity for hydrophilic molecules 0
Hydrophobicity
2 to 8 14
Low High
Retains highly polar molecules that are not retained by Pore Size
reversed-phase chromatography
0 120 300
Unique selectivity, complementary to reversed-phase columns
Particle Size 3m, 5m
Hydrolytically stable
Rugged column packing
Broad application range
The Acclaim HILIC-10 column is designed for separating highly hydrophilic molecules by Hydrophilic
Interaction Liquid Chromatography (HILIC). This column is based on high-purity spherical porous silica
covalently modified with a proprietary hydrophilic layer.
Acclaim
Glycerides
200
Acclaim HILIC-10, 3m, 150 x 3.0mm
Mobile Phase A: Heptane
Mobile Phase B: 2-Propanol/acetic acid 99.5:0.5
Temperature: 25C
2
1 3 Detection: Corona ultra, nebulizer 15C
Analytes: 1. Tristearin
pA
7 2. Trilaurin
8 3. Distearin isomer 1
5 4. Dilaurin isomer 1
5. Distearin isomer 2
6. Dilaurin isomer 2
7. Monostearin
6 8. Monolaurin
0
0 2 4 6 8 10
Minutes
Acclaim HILIC-10
3 HPLC Column 150 074259 074258 074257
5 Guard Cartridge 10 074263 074261 074262

Format Cat. No.
Acclaim Mixed-Mode HILIC-1
pH Range
Uniquely designed for both reversed-phase and HILIC operations 0 2.5 to 7.5 14
Hydrophobicity
Low High
Can operate in both RP and HILIC modes
Pore Size
Retains highly polar molecules
0 120 300
Unique selectivity complementary to RP columns
Particle Size 3m, 5m
Broader application range compared with conventional diol-based columns
High-efficiency column for high-resolution separations
The Acclaim Mixed-Mode HILIC-1 column features a unique, high-efficiency, silica-based

HPLC mixed-mode stationary phase that combines both reversed-phase (RP) and hydrophilic
interaction liquid chromatography (HILIC) properties. This combination allows both
hydrophobic and hydrophilic interactions to be utilized to optimize separations.
The functional group is of a hydrophobic alkyl chain with a diol group at the terminus. This
unique combination results in the adjustable selectivity, making Acclaim Mixed-Mode HILIC-1
separate mixtures that would be impossible for a C18 column. This column is suitable for a
broad range of applications, including non-ionic ethoxylated surfactants, drug metabolites,
lipids, polyethylene glycols (PEGs), ethoxylated surfactants, and more.
Cytosine and naphthalene
1
Acclaim Mixed-Mode HILIC-1, 5m, 150 x 4.6mm
Reversed-Phase Mode
Mobile Phase: CH 3CN/0.1 M NH 4OAc, pH 5.2
2 v/v 52/48 for RP mode
v/v 92/8 for HILIC mode
Temperature: 30C
AU
Flow Rate: 1mL/min

Analytes: 1. Cytosine (100 ppm)
HILIC Mode 2. Naphthalene (100 ppm)
0 2 4 6 8 10
Minutes
Acclaim Mixed-Mode HILIC-1

3 HPLC Column 50 - 071912 -
150 070091 070090 -
5 Guard Cartridge 10 069694 071913 069706
HPLC Column 150 066847 - 066843
250 - - 066844

Format Cat. No.
4-042
Acclaim Mixed-Mode WAX-1

pH Range
Designed for separating anionic molecules with powerful adjustable 0 2.5 to 7.5 14
Hydrophobicity
selectivity control
Low High
Pore Size
Adjustable selectivity
Selectivity orthogonal to reversed-phase (RP) columns 0 120 300
Ideal selectivity for anionic molecules Particle Size 3m, 5m USP L78
Excellent column efficiency and peak asymmetry

Multimode retention mechanisms: reversed-phase, weak anion exchange,
and HILIC modes
The Acclaim Mixed-Mode WAX-1 is a novel, high-efficiency silica HPLC column that
combines hydrophobic and weak anion exchange characteristics. Its unique chemistry
results in a multimode separation mechanism that includes reversed-phase, anion
exchange, and HILIC interactions. Selectivity can be adjusted by changing ionic strength,
pH or organic solvent content.
Acclaim
Pain relief medicine
Acclaim Mixed-Mode WAX-1, 5m, 150 x 4.6mm

Mobile Phase: 40/60 v/v Acetonitrile/buffer
3 (6.8 g potassium monophosphate and
0.5 g pyrophosphate in 1000 g D.I. H2 O,
pH is adjusted to 6.0 with NaOH)
Temperature: 30C
Flow Rate: 1mL/min
2
AU 1 Analytes: 1. Caffeine
4 2. Acetaminophen
3. Salicylamide
O OH 4. Acetyl salicylic acid (Aspirin)
OH 5. Salicylic acid
0 10 20
Minutes
Acclaim Mixed-Mode WAX-1

3 HPLC Column 50 - 071908 -
150 070089 070088 -
5 Guard Cartridge 10 069686 071909 069704
HPLC Column 150 067084 - 064984
250 - - 064985

Format Cat. No.
Acclaim Mixed-Mode WCX-1
pH Range
Designed for separating cationic molecules with adjustable 0 2.5 to 7.5 14

Hydrophobicity
selectivity control
Low High
Pore Size
Adjustable selectivity
Ideal selectivity for separating basic molecules 0 120 300
Selectivity complementary to C18 RP columns Particle Size 3m, 5m USP L78
Multimode separation mechanism: reversed-phase, weak cation exchange,

anion-exclusion and HILIC
The Acclaim Mixed-Mode WCX-1 is a novel, high-efficiency, silica-based column, with

a proprietary ligand with both hydrophobic and weak cation exchange properties.
Selectivity of ionizable and neutral compounds can be controlled independently or
simultaneously by tuning mobile phase ionic strength, pH or organic modifier. This
column therefore can separate using multiple separation modes: reversed-phase,
cation exchange, and normal-phase/ HILIC and is recommended for a variety of
industrial applications, including pharmaceutical, chemical, consumer products,
foods and beverages.
Pharmaceutical counterions
Acclaim Mixed-Mode WCX-1, 5m, 150 x 4.6mm

1 Mobile Phase: 40/60 v/v CH 3 CN/NH 4 OAc,
pH 5.2 (20 mM total)
3 Temperature: 30C
Flow Rate: 1mL/min
7 Detection: UV (225 nm)
Analytes: 1. Maleate 50g/mL
AU 2. Ketoprofen 30g/mL
2 3. Naproxen 30g/mL
5 4. Hydrocortisone 60g/mL
5. Dexamethasone 60g/mL
4 6. Oxprenolol 300g/mL
6
7. Timolol 250g/mL
0 4 8
Minutes
Acclaim Mixed-Mode WCX-1

3 HPLC Column 50 - 071910 -
150 070093 070092 -
5 Guard Cartridge 10 085455 071911 069705
HPLC Column 150 068371 - 068353
250 - - 068352

Format Cat. No.
4-044
OmniPac

pH Range
DVB polymer columns for combined ion exchange and 0 14
reversed-phase separations Pore Size
0 60 1200 1500
PAX-100 PAX-500
Acid, base and solvent compatible, pH 0 to 14

PCX-100 PCX-500
Ideal for the separation of high molecular weight organic acids

Delivers optimal separation of very hydrophobic anions
Delivers optimal separation of halogenated anions
Provides simultaneous separation of neutral and ionic species
Unique selectivity for polar and ionic organic analytes
Delivers optimal separation of organic, hydrophobic, and halogenated cations
Thermo Scientific OmniPac is a range of latex-based columns. Both PAX columns have an
ion exchange capacity of about 40eq per column, and the PCX columns have a capacity of
approximately 120eq per column. The PAX-500 and PCX-500 columns separate analytes
through both ion exchange and reversed-phase mechanisms, due to their higher reversed-
phase capacity relative to the PAX-100 and PCX-100 columns.
OmniPac
Gradient separation of nitrogen-containing compounds
16
12 OmniPac PCX-500, 250 x 4.0mm
13 Mobile Phase: Acetonitrile/Sodium Chloride/
9 Hydrochloric Acid Gradient
6,7 Flow Rate: 1.0mL/min
Analytes: 1. Orotic Acid
1 2 4,5
11 2. 4-Hydroxybenzamide
10
3. Luminol Impurity
17 4. Luminol
5. Pyridine
8 14 15 6. PABA
3 7. 2,2-Bipyridine
8. p-Phenylenediamine
9. Naphthylamine
0 5 10 15 10. Nitrobenzoic Acid
Minutes 11. Tribenzylamine
12. p-Nitroaniline
13. 2,4-Dinitroaniline
14. Dibenzylamine
15. N-Methyl-N-nitrosoaniline
16. 4-Chloro-2-nitroaniline
17. 2,6-Dichloro-4-nitroaniline
OmniPac Anion Exchange

Description Porosity Length (mm) 4.0mm ID
PAX-100 Microporous 50 042151
250 042150
PAX-500 Microporous 50 042153
250 042152
OmniPac Cation Exchange

Description Porosity Length (mm) 4.0mm ID
PCX-100 Microporous 50 042193
250 042189
PCX-500 Microporous 50 042195
250 042191
Acclaim Size Exclusion Chromatography (SEC) pH Range
High performance SEC columns for analysis of water soluble polymers 0 2 to 12 14
Pore Size
0 1500
Proprietary mono-dispersed multi-pore hydrophilic resin: no inflection points 300 (SEC 300) 1000 (SEC 1000)
in calibration curve Particle Size 5, 7m
SEC-300 calibrated from 100 to 50,000 Daltons
SEC-1000 calibrated from 1,000 to 1,000,000 Daltons
Availability of small particle sizes packed in 300 x 4.6mm dimension allows for
high-resolution analysis at reduced solvent consumption
Stable surface bonding with low column bleed and compatibility with UV, RI, MS,
ELSD and Thermo Scientific Dionex Corona Charged Aerosol Detectors
Thermo Scientific Acclaim SEC-300 and SEC-1000 are a family of resin based, high performance size
exclusion chromatography columns specifically designed for the separation of water soluble polymers
and oligomers.
100,000,000
SEC-1000 Acclaim SEC-300, 5m, 300 x 4.6mm

10,000,000 Acclaim SEC-1000, 7m, 300 x 4.6mm
SEC-300
Mobile Phase: 10mM sodium perchlorate
1,000,000 Temperature: 25C
Molecular Weight (Da)

100,000
Detection: RI
Analytes: (0.03% - 0.1% in mobile phase)
10,000 dextran (MW 5,000,000-
40,000,000), PEO (MW 895,000,
580,000, 272,000, 185,000, 80,000,
1000
43,000, and 20,000), PEG (MW
8,300, 3,000, 1,500, 600, 400 and
100 200), diethylene glycol (MW 106
and ethylene glycol (MW 62)
10
1.50 2.00 2.50 3.00 3.50 4.00 4.50
Elution Volume (mL)
Polyacrylic acid using size-exclusion chromatography with charged-aerosol detection
2.5 Acclaim SEC-300 , 5m, 300 x 4.6mm

Polyacrylic acid
pA 0.450 Mobile Phase A: Acetonitrile
PAA
Mobile Phase B: Water
Area [ pA *min]
Temperature: 30C
0.200
400 g/L Detection: Corona III; evaporator 55C, Engine
Concentration [ g/L] 40 C, 2 Hz, filter 5, power function
200 g/L 0.00c0 1.20
0 125 250 375 500 Analytes: 1. PAA standards in water
100 g/L
50 g/L
25 g/L
-0.0
0 5 Minutes 10 15
Acclaim Size Exclusion Chromatography (SEC)

Description Particle Size (m) Format Length (mm) 4.6mm ID 7.8mm ID
Acclaim SEC-300 5 Guard 33 082740
HPLC Column 150 079726
300 079723 079725
Acclaim SEC-1000 7 Guard 33 082739
300 079724 079721
4-046
Notes
Application Specific HPLC

and UHPLC Columns
Innovative chemistries tailored for challenging

and critically important applications
Application specific columns utilize novel and unique chemistries to provide superior
resolution with ease of use for key pharmaceutical and environmental applications.
Acclaim AmG C18 Acclaim Trinity Q1

Aminoglycoside antibiotics separation Diquat and paraquat analysis
Acclaim Trinity P1 and P2 Acclaim Carbamate

API & counterion analysis The separation of carbamate pesticides
Acclaim Organic Acid Acclaim Carbonyl C18

Fast organic acid analysis Separation of DNPH derivatives of aldehydes
and ketones
Acclaim Surfactant and
Surfactant Plus
Separation of surfactants
Acclaim Explosives
Separation of explosive residues
The Application Specific column web page contains

the latest news, applications and downloads for the
Application Specific HPLC column range.
Visit www.thermoscientific.com/columns
4-048
Acclaim AmG C18

Designed to provide rugged and reproducible reversed-phase
chromatography of aminoglycoside antibiotics.
Rugged and reproducible

Excellent selectivity for the HPLC of aminoglycosides
Superior resistance to acidic conditions for long column lifetime
Easy to use with only aqueous mobile phase; TFA only, or TFA/HFBA or PFPA is needed
Compatible with simple rugged methods; no solvents are required
High efficiency and throughput
Aminoglycoside antibiotics are commonly used as clinical and veterinary medicines to treat bacterial
infections. HPLC using ion-pairing reversed-phase separations is an effective technique for simultaneous
qualitative and quantitative determination of aminoglycosides.
Acclaim
The Acclaim AmG C18 column is designed to provide excellent stability, selectivity and high resolution. It
has a unique surface, a polymer encapsulated silica covalently bonded with a C18 ligand. This ensures
ultra-stability when exposure to low pH (<1) and high temperature separation conditions.
Isocratic separation of gentamicin sulfate using 100 mM TFA as the mobile phase
10.0
C1a Acclaim AmG C18, 3m, 150 x 3.0mm
Mobile Phase: 100 mM TFA
Temperature: 30C
R1 R2 R3
7.5 Flow Rate: 0.425 mL/min
C 1a H H H
C2 H CH 3 H Injection Volume: 2L
C 2b CH 3
H H Detection: Corona Veo RS (Filter =
C 2a H H CH 3 5.0 s; Evaporation Temp =
C1 CH CH H
3 3
35 C; Data Rate = 5 Hz;
5.0 C2
Current [pA]
Power Function = 1.00)

C2a Sample: Gentamicin (1 mg/mL)
C1
2.5
C2b
0.0
0.0 10.0 20.0 30.0 40.0 50.0 60.0

Retention Time [min]
Acclaim AmG C18

3 Guard Cartridges 10 088754 088756 088758
(2/pk)
HPLC Column 150 088753 088755 088757
Guard Cartridge Holder 069580 069580 069580
Acclaim Trinity P1
Mixed mode column technology combining reversed-phase, anion exchange

and cation exchange functionality on a single support
Ideal selectivity for simultaneous separation of API and counterion

Adjustable selectivity by mobile phase ionic strength, electrolyte type, pH, and organic solvent
Low bleed; compatible with MS, CAD and ELSD
Retention of hydrophilic ionic and ionizable analytes without ion-pairing reagents
Greater flexibility in method development: each retention mechanisms can be controlled independently
The Thermo Scientific Acclaim Trinity P1 HPLC column is designed with Nanopolymer Silica Hybrid (NSH) technology, which results in a multimode
surface chemistry ideal for the simultaneous separation of drugs and their counterions. The surface chemistry concurrently provides reversed-phase,
cation exchange, and anion exchange functionalities. The result is maximum flexibility in method development. Separations can be optimized
easily by adjusting the chromatographic parameters (mobile phase pH, ionic strength, and organic strength).
Simultaneous separation of pharmaceutical counterions

300 Acclaim Trinity P1, 3m, 100 x 3.0mm
Mobile Phase: 60/40 v/v CH 3CN/20mM (total)
NH 4OAc, pH 5
Temperature: 30C
3 Detection: Corona ultra (Gain = 100 pA;
Filter = med; Neb Temp = 30C)
mV
1 Analytes: 1. Choline
4 (50 to 100ppm) 2. Tromethamine
2 3. Sodium
4. Potassium
5. Meglumine
8
6 6. Mesylate
5
7. Nitrate
7 9 10 8. Chloride
9. Bromide
0 10. Iodide
0 3 6 9 12 15
Minutes
Acclaim Trinity P1
Particle Size (m) Format Length (mm) 2.1mm ID 3.0mm ID

3 Guard Cartridges 10 071391 071390
(2/pk)
HPLC Column 50 075565 071388
100 071389 071387
150 075564 075563

Format Cat. No.
Acclaim SST Guard Cartridge Holder V-2 069580
Acclaim Guard Kit (Holder and coupler) V-2 069707
4-050
Acclaim Trinity P2

Mixed-mode column technology; hydrophilic interaction combining HILIC,
anion exchange and cation exchange functionalities
Ideal for separating pharmaceutical counterions, including monovalent

and divalent cations or anions
Selectivity complementary to the Trinity P1 column
Low column bleed, compatible with CAD and MS
Hydrolytically stable
High efficiency
The Acclaim Trinity P2 is a unique, high-efficiency, silica-based column specifically designed for separation of pharmaceutical counterions, including
monovalent and divalent cations or anions. This column is based on Nanopolymer Silica Hybrid (NSH) technology, which consists of high-purity
porous spherical silica particles coated with charged nanopolymer particles. The inner-pore area of the silica bead is modified with a covalently
bonded organic layer that provides cation-exchange retention, while the outer surface is modified with anion-exchange nano-polymer beads.
Acclaim Trinity P2 column is aimed to complement Acclaim Trinity P1 to provide a total solution for pharmaceutical counter ion analysis by HPLC.
Acclaim
Pharmaceutical-related anions and cations
Acclaim Trinity P2, 3m, 100 x 3.0mm
5.0 Mobile Phase: D.I. water and 100 mM NH4OFm, pH
3.65 gradient
Temperature: 30C
Flow Rate: 0.60 mL/min
Detection: Corona Veo Charged Aerosol Detector
1
Analytes: 1. Phosphate
2 2. Sodium
3. Potassium
10 4. Chloride
5. Malate
6. Bromide
6 11 7. Nitrate
9
pA 8. Citrate
9. Fumarate
10. Sulfate
5 8 11. Magnesium
3 12. Calcium
Samples: 0.02 0.10 mg/mL each in D.I. water
12
4
7
Time (min) H2O 0.1 M Ammonium formate, pH3.65
-10 0.760 1.474
0 80 20
0
2 80 20
0 6 12 18 24 30 22 0 100
Minutes 30 0 100
Acclaim Trinity P2
Particle Size (m) Format Length (mm) 2.1mm ID 3.0mm ID
(2/pk)
HPLC Column 50 085431 085433
100 085432 085434

Format Cat. No.
Acclaim Organic Acid
Optimized and application-tested for the analysis of hydrophilic organic acids
Tested to guarantee consistent hydrophilic organic acid separations

Compatible with 100% aqueous mobile phases
Hydrolytic stability at low-pH conditions
Ideal selectivity for separating a wide spectrum of organic acids
Excellent column efficiency and peak shapes for organic acids
The Acclaim Organic Acid (OA) is a silica-based reversed-phase column designed for high-efficiency, high-throughput organic acids analysis.
It offers unparalleled performance for separating hydroxyl aliphatic and aromatic organic acids.
The Acclaim OA is the recommended column for determining small hydrophilic organic acids, C1 to C7 aliphatic acids, and hydrophilic aromatic
acid and is also valuable for the analysis and quality assurance of food and beverage products, pharmaceutical preparations, plating baths, and
manufacturing chemicals, chemical intermediates, and environmental samples.
Hydrophilic organic acids

Acclaim Organic Acid, 5m, 4 250mm
0.025 Mobile Phase: 100mM Na 2SO 4, pH 2.65
10 (adjusted with methanesulfonic acid)
Temperature: 30C
AU
2
Analytes: 1. Oxalic acid 15mg/L (ppm)
2. Tartaric acid 120
3. Formic acid 180
1 3 4 12 4. Malic acid 120
8 5. iso-Citric acid 120
567 9 11 6. Lactic acid 180
7. Acetic acid 120
0
8. Citric acid 120
0 5 10 15 9. Succinic acid 120
Minutes 10. Fumaric acid 7
11. cis-Aconitic acid *
12. trans-Aconitic acid *
* 7ppm total for cis and trans isomers
Acclaim Organic Acid

3 HPLC Column 150 070087 070086

(2/pk)
250 062902

Format Cat. No.
4-052
Acclaim Surfactant

Excellent performance for separating a broad range of surfactants
Ideal selectivity for separation of anionic, nonionic, cationic and

amphoteric surfactants
Excellent peak shapes, especially for cationic surfactants
Compatible with highly aqueous mobile phases
Improved resolution for ethoxylated surfactants
Rugged separations under a variety of conditions
The Acclaim Surfactant columns are the first generation high-efficiency, silica-based columns designed specifically for separating a wide variety of
surfactants, including anionic, cationic, nonionic, ethoxylated and amphoteric surfactants using UV, ELSD or RI detection.
Surfactants are widely used in industrial, agricultural, and pharmaceutical markets, in products as diverse as pesticides, detergent powders,
petroleum products, cosmetics, and pharmaceuticals. The Acclaim Surfactant column was designed specifically for HPLC separation
of these surfactants.
Acclaim
Inorganic anion, hydrotropes, cationic, nonionic, amphoteric, and anionic surfactants
Acclaim Surfactant, 5m, 150 x 4.6mm

Mobile Phase A: CH 3 CN,
6 Mobile Phase B: 0.1 M NH 4 OAc, pH 5.4
5 Gradient: 25% to 85% A in 25min,
10 then hold 85% A for 10min
Temperature: 30C
mV
3
Flow Rate: 1mL/min
2 4 Injection Volume: 25L
7 9 13 Detection: ELS detector
8
1 12 Analytes: 1. Chloride
11 14 2. Bromide
3. Nitrate
4. Xylene sulfonate
0 5 10 15 20 25 30 35 5. Laurylpyridinium chloride
Minutes 6. Lauryldimethylbenzyl-ammonium chloride
7. Triton X-100
8. Cetyl betaine
9. Decyl sulfate
10. Dodecyl sulfate
11. C10 -LAS
12. C11-LAS
13. C12 -LAS
14. C13 -LAS
Acclaim Surfactant
3 HPLC Column 150 070085 070084
(2/pk)
HPLC Column 150 068123 063201
250 063203

Description Cat. No.
Acclaim Surfactant Plus
Column of choice for surfactant analysis using higher sensitivity detection:

performance, versatility, throughput
Ideal selectivity for simultaneous separation of anionic, nonionic, cationic,

and amphoteric surfactants
Compatible with multiple detectors including MS, CAD, ELSD and UV
Well suited for the determination of cationic surfactants
High efficiency and fast analysis
Rugged separations under a variety of conditions
Acclaim Surfactant Plus is a new generation of columns offering improved performance and higher throughput for analyzing surfactants. These
columns exhibit exceptionally low bleed and are ideal for use with charged aerosol detectors (CAD) and mass spectrometers (MS). These columns
can be used to separate a wide variety of surfactants including anionic, cationic, nonionic and amphoteric surfactants, as well as isomers of
xylene sulfonate.
These columns can be used with evaporative light scattering detectors (ELSD), suppressed conductivity detectors (SCD), and UV-Vis detectors (UV).
Non-metallic PEEK hardware is available for best compatibility with Dionex ion chromatography systems.
Cationic surfactants Acclaim Surfactant Plus, 3m, 150 x 3.0mm

10 1 Mobile Phase A: Acetonitrile
Mobile Phase B: 100mM Formic acid
S Mobile Phase C: Water
Gradient:
Time
%A %B %C
3 (min)
2 -12 5 5 90
4
0 5 5 90
12 40 5 55
0 20 40 5 55
Temperature: 25C
5 6
5 Detection: Conductivity with blank subtraction
S 8 Analytes: 1. Tetrabutylammonium
2. Tetrapentylammonium
3. Tetrahexylammonium
4. Tetraheptylammonium
5. Decyl-trimethylammonium
0 6. Dodecyl-trimethylammonium
7. Tetradecyl-trimethylammonium
8. Hexadecyl-trimethylammonium
0 5 10 15
Minutes
Acclaim Surfactant Plus

Particle Size (m) Format Length (mm) 2.1mm ID 3.0mm ID 4.6mm ID 4.0mm ID PEEK
3 HPLC Column 100 078955 078952
150 078954 078951 078950
250 078953
(2/pk)
150 082768 078956

4-054
Acclaim Explosives E2

The best solution for explosives analysis (EPA Method 8330)
Acclaim E2 columns provide baseline resolution

of all 14 compounds targeted by EPA Method 8330
Columns available in 2.2, 3 and 5m particle size
Simple isocratic elution conditions
Rugged columns with good lot-to-lot reproducibility
Acclaim Explosives E2 columns are specifically designed to resolve all 14 explosives listed in EPA SW-846 Method 8330: Nitroaromatics and
Nitramines by HPLC. The novel and unique chemistries of these columns provide superior resolution with complementary selectivities.
The Acclaim Explosives E2 may be used as either a primary or a confirmatory column. The unique selectivity and versatility of this column
provides a wider application range, including the analysis of explosives beyond U.S. EPA Method 8330 (ISO22478).
Rapid determination of EPA 8330A explosives
Acclaim
60
4 Acclaim RSLC Explosives E2, 2.2m, 100 x 2.1mm
Mobile Phase: Methanol:water 48:52 (v/v)
3
Temperature: 31C
Flow Rate: 0.34mL/min (293 bar)
5 9 Detection: UV, 254nm
6 Analytes: 1. HMX 8. 2,6-DNT
mAU
2. RDX 9. 2,4-DNT
2 3. 1,3,5-TNB 10. 2-NT
8
1 7 14 4. 3,5-DNB 11. 4-NT
10 12 13 5. NB 12. 3-NT
11 6. 2,4,6-TNT 13. 4-Am-2,6-DNT
7. Tetryl 14. 2-Am-4,6-DNT
Sample: Calibration mix, 25g/mL
0 in 50% acetontrile
0 2.5 5 7.5 10
Minutes 28815
Acclaim Explosives E2
2.2 RSLC Column 100 076225 076227
150 076226
3 HPLC Column 150 070083 070082
250 070081
(2/pk)
Acclaim Trinity Q1
For trace analysis of diquat and paraquat
Excellent resolution of diquat and paraquat

Good peak shape
Fast analysis
LC-MS compatible
No ion-pairing reagent needed
Acclaim Trinity Q1 columns are unique, high-efficiency, silica-based columns designed for the separation of the herbicides diquat and paraquat.
These herbicides are toxic and residues are monitored in drinking water, wastewater and agricultural products. The Acclaim Trinity Q1 column is a
tri-mode (WCX, WAX, RP), column based on Nano-polymer Silica Hybrid technology. It offers unmatched high-resolution and high-throughput trace
analysis of the herbicides diquat and paraquat by LC-MS/MS and LC-UV methods.
Diquat and paraquat
Acclaim Trinity Q1, 3m, 50 x 3.0mm

Paraquat: Q-SRM Mobile Phase: 25% ammonium acetate
1.19E5
(100mM, pH 5.0); 75%
acetonitrile
185/169 Temperature: Ambient
Paraquat: C-SRM Injection Volume: 5L
5.46E4 Detection: Show Mass Spectrometric
conditions and the scan events
etc. table underneath are the
185/170 peaks section
Mass Spectrometric Conditions
Paraquat-IS System: Thermo Scientific TSQ Quantiva
5.47E4 Access MAX Quadrupole
Mass Spectrometer
Relative Abundance
Interface: Heated Electrospary Ionization

193/178
with HESI II probe
Spray Voltage: 1500 V
Diquat: Q-SRM Vaporizer Temp: 400 C
9.67E5
Sheath Gas Pressure: 70
Aux Gas Pressure: 10
183/157 Capillary Temp: 350 C
Quantitation Mode: Selected Reaction Monitoring
Diquat: C-SRM (SRM )
3.11E5
Scan Events Precursor Quantitative Confirmative
183/130 SRM (CID) SRM (CID)

Paraquat 185 169 (27) 170 (17)
Diquat-IS
9.98E5 Paraquat-d6 193 178 (17)
Diquat 183 157 (22) 130 (31)
186/158 Diquat-d3 186 158 (22)
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0
Time (min)
Acclaim Trinity Q1
Particle Size Length (mm) 2.1mm ID 3.0mm ID
3 HPLC Column 50 083242 083241
100 079717 079715
(2/pk)
4-056
Acclaim Carbamate

Designed for baseline separation of carbamate pesticides
specified in US EPA Method 531.2
Baseline separation of carbamate pesticides specified in US EPA Method 531.2

Use with either LC/postcolumn derivatization/fluorescence or LC-MS detection
Available in 2.2, 3 and 5m particle size
Compatible with both binary (methanol/water) and ternary (acetonitrile/methanol/water)
mobile phase gradients
High-efficiency, extremely low column bleed, and rugged column packing
Acclaim Carbamate columns are designed for baseline separation of carbamates (N-methylcarbamate and N-methylcarbamoyloxime pesticides)
specified in US EPA Method 531.2. Carbamate pesticides are widely used throughout the world. Drinking water and raw surface water is
monitored for the presence of carbamate pesticides and related compounds using an established EPA Method 531.2 that uses HPLC with
postcolumn derivatization. LC-MS is the method of choice for the ultimate sensitivity.
Carbamate standard - spiked rice samples
Acclaim
Acclaim Carbamate, 3m, 150 x 3.0mm
Mobile Phase: MethanolH 2 O
4000 Gradient: Methanol, -4.00.0 min, 14%;
4 A 2.0 min, 20%; 8.0 min, 40%;
1 13.616 min, 70%
2 9
3 Temperature: 50 C
6 10
counts 5 Flow Rate: 0.9mL/min
7 8 12 Injection Volume: 250L
11 Detection: Excitation/330nm and
1 Emission/465nm
Analytes: 1. Aldicarb sulfoxide
-500 2. Aldicarb sulfone
0 1.3 2.5 3.8 5 6.3 7.5 8.8 10 11.3 12.5 13.8 15 16 3. Oxamyl
2000 4. Methomyl
5. 3-Hydroxy carbofuran
12 B 6. Aldicarb
7. Propoxur
8. Carbofuran
counts
9. Carbaryl
2 1 2 3 4 9
10. 1-Naphthol
5 6 7 8 10 11. Methiocarb
11
12. BDMC (I.S.)
-300
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
Minutes
A: without dispersive SPE

B: with dispersive SPE using PSA
Acclaim Carbamate
2.2 RSLC Column 100 075597
150 075596
(2/pk)
HPLC Column 150 072927 072926 072925
5 HPLC Column 250 072924

Acclaim Carbonyl C18
A silica-based, reversed-phase column designed specifically

for separating DNPH derivatives of aldehydes and ketones
Ideal selectivity for baseline resolution of DNPH derivatives of aldehydes

and ketones regulated by various official methods, including EPA 554,
EPA 8315, EPA 1667, EPA TO-11, and CARB 1004
High efficiency for UHPLC performance
Rugged columns with good lot-to-lot reproducibility
Proven robust methods
Acclaim Carbonyl C18 columns are silica-based reversed phase columns designed specifically for separating DNPH derivatives of aldehydes and
ketones. They exhibit superior resolution compared with other commercially available columns.
Aldehydes and ketones are common pollutants in air and water. Several standard methods have been developed to apply using dinitrophenylhydrazine
(DNPH) to various environmental situations to measure these compounds. Some of the better known ones include CARB 1004 for vehicle exhaust, EPA
554 for drinking water, EPA 1667 for pharmaceutical wastewater, and EPA 8315 for general wastewater.
DNPH aldehydes and ketones

Acclaim Carbonyl RSLC, 2.2m, 150 x 2.1mm
300 Mobile Phase A: D.I. water
10 11 12
9 Gradient (min): -4.5 0.0 8.3 15.0 18.0
8 %A 48 48 48 0 0
1 %B 52 52 52 100 100
Temperature: 28C
2
5 Samples: Calibration mixes diluted in methanol
Analytes: 1. Formaldehyde DNPH
3
2. Acetaldehyde DNPH
4 3. Propionaldehyde DNPH
4. Crotonaldehyde DNPH
mAU
5. Butyraldehyde DNPH
EPA 6. Cyclohexanone DNPH
554 7. Valeraldehyde DNPH
8. Hexanal DNPH
9. Heptanal DNPH
13 14 15 16 17 19 10. Octanal DNPH
CARB 11. Nonanal DNPH
12. Decanal DNPH
1004 13. Acetone DNPH
14. Acrolein DNPH
15. Butanone DNPH
18 20
19
16. Methacrolein DNPH
EPA 17. Benzaldehyde DNPH
TO-11 18. Isovaleraldehyde DNPH
0 19. Tolualdehyde DNPH
20. Xylylaldehyde DNPH
0 5 10 15 18
Minutes
Acclaim Carbonyl C18

2.2 RSLC Column 100 077972 077974
150 077973
3 HPLC Column 150 079011 079010
250 079009
5 Guard Cartridge 10 079012 079013 079014
(2/pk)
250 083214
4-058
Hypercarb HPLC Columns
100% porous graphitic carbon for extended
separation capabilities
Hypercarb
Used for the retention and separation of highly polar species. Thermo Scientific
Hypercarb columns are ideally suited to solve in both reversed phase and normal
phase HPLCand LC-MS applications.
Exceptional Retention
of Very Polar Analytes
Ideal for complex separations
Separates Structurally
Related Substances
More effective than silica-based columns
pH Stable from 0 to 14
Extended temperature and pressure capabilities
The Hypercarb web page contains the latest news, applications

and downloads for the Hypercarb HPLC column range.
Visit www.thermoscientic.com/hypercarb
Increased Retention of Polar Analytes
PS-DVB Hypercarb
In typical reversed phase chromatography,

Courtesy V. Coquart and
the retention of an analyte is directly related M-C. Henion, J. Chrom., 1992
to its hydrophobicity: the more hydrophobic
the analyte, the longer its retention. Conversely,
as the polarity of the analyte increases,
analyte-solvent interactions begin to dominate
and retention is reduced. This observation holds
true for the majority of reversed phase systems.
An exception to this rule is Hypercarb columns,
for which retention may in some cases increase
as the polarity of the analyte increases,
illustrated to the right. This phenomenon
is referred to as the polar retention effect Retention on Hypercarb columns increases as polarity of the analyte increases, which is the opposite of typical reversed
on graphite (PREG). This property makes phase materials such as PS-DVB
Hypercarb columns particularly useful for the

Extended pH Range with typical silica-based columns. Hypercarb
separation of highly polar compounds (with
One of the other key benefits of Hypercarb columns offer more choice in buffer selection
logP as low as -4) that are normally difficult
columns is the extreme stability of the phase while handling both high temperature and
to retain and resolve on silica-based alkyl
to chemical or physical attack. Due to the high pressure.
chain phases. The retention of very polar
unique characteristics of the media, it can
solutes on Hypercarb columns can be
withstand chemical attack across the entire
achieved without ion pair reagents or complex
pH range of 0 to 14, allowing applications to
mobile phase conditions, as illustrated
be run at pH levels that are incompatible
in the chromatogram below.
Hypercarb, 5m, 100 x 0.32mm

Mobile Phase A: H 2O + 0.1% formic acid
Mobile Phase B: ACN + 0.1% formic acid
columns Gradient: 0 to 25% B in 15 minutes
Temperature: 25C
Flow Rate: 8L/min
Analytes: 1. Cytosine
2. Uracil
3. Guanine
4. Adenine
5. Xanthine
6. Thymine
Additional retention is achieved for polar compounds using a Hypercarb column compared to a polar endcapped C18.
Note also the change in elution order.
columns
1 1
4
2
2 Day 1 at pH 12 Day 93 at pH 12 Hypercarb, 5m, 100 x 4.6mm

Mobile Phase: MeOH:H 2O
4 Gradient: 70:30
3 Analytes: 1. Acetone
2. Phenol
5 3. p-Cresol
4. Anisol
5
5. Phenetole
6. 3,5 -Xylenol
6
6
PGCpH12
0 10 20 Min 0 10 20 Min
Hypercarb column stability at pH 12: retention and selectivity do not change even after 93 days of storage in 0.1M NaOH/MeOH
4-060
Hypercarb
Particle Size (m) Format Length (mm) ID (mm) Cat. No.
3 Drop-in Guard (4/pk) 10 2.1 35003-012101
3.0 35003-013001
4.6 35003-014001
HPLC Column 30 1.0 35003-032130
3.0 35003-033030
50 2.1 35003-052130
3.0 35003-053030
4.6 35003-054630
100 2.1 35003-102130
3.0 35003-103030
4.6 35003-104630
150 2.1 35003-152130
3.0 35003-153030
4.6 35003-154630
High Temperature HPLC Column 30 2.1 35003-032146
50 2.1 35003-052146
4.6 35003-054646
100 2.1 35003-102146
3.0 35003-103046
4.6 35003-104646
5 Drop-in Guard (4/pk) 10 2.1 35005-012101
3.0 35005-013001
Hypercarb
4.6 35005-014001
HPLC Column 30 2.1 35005-032130
3.0 35005-033030
4.6 35005-034630
50 2.1 35005-052130
3.0 35005-053030
4.6 35005-054630
100 2.1 35005-102130
3.0 35005-103030
4.6 35005-104630
150 2.1 35005-152130
3.0 35005-153030
4.6 35005-154630
High Temperature HPLC Column 30 2.1 35005-032146
4.6 35005-034646
50 2.1 35005-052146
4.6 35005-054646
100 2.1 35005-102146
4.6 35005-104646
Javelin HTS Column 20 2.1 35005-022135
Preparative HPLC Column 100 10 35005-109070
21.2 35005-109270
30 35005-109370
150 10 35005-159070
21.2 35005-159270
Format Length (mm) ID (mm) Cat. No.

UNIGUARD Guard Cartridge Holder 10 1.0 851-00
2.1 852-00
3.0 852-00
4.6 850-00
Syncronis HPLC
and UHPLC Columns
Remarkable separations guaranteed time

after time
Thermo Scientific Syncronis HPLC columns are used to achieve consistent,
reproducible separations. Chemistries available for a variety of applications.
Consistent Separations
Highly pure, high surface area silica
Dense Bonding and

Double Endcapping
Reduction of residual silanols available
to interact with basic analytes
Strong Quality Control

Rigorously tested to ensure quality
The Syncronis web page contains the latest news, applications

and downloads for the Syncronis HPLC column range.
Visit www.thermoscientic.com/syncronis
4-062
Syncronis C18 pH Range

Syncronis C18 columns deliver remarkable separations, 0 2 to 9 14
Hydrophobicity
time after time
Low High
Pore Size
0 100 300
High carbon load for increased retention
Carbon Load (%)
Double endcapped for extra surface coverage
Highly inert towards basic compounds 0 16 25

Syncronis C18 columns show excellent column to column reproducibility,

as illustrated here by the analysis of zidovudine using five separate columns.
The reproducibility in terms of retention time and peak area is less than or equal
to 0.5%, column to column.
Ziovudine
Syncronis
2400 Syncronis C18, 5m, 150 x 4.6mm
Mobile Phase: Water:Methanol (4:1)
2000 Temperature: 25C
1600
mAU
1200
Analytes: 1. Zidovudine
800
400
0
0 4 8 12 16 20 24 28 32
Minutes
Syncronis C18
1.7 UHPLC Column 30 97102-032130 - -
50 97102-052130 - -
100 97102-102130 - -
3 HPLC Column 30 97103-032130 - -
50 97103-052130 - 97103-054630
100 97103-102130 97103-103030 97103-104630
150 97103-152130 - 97103-154630
5 Drop-in Guard (4/pk) 10 97105-012101 97105-013001 97105-014001
HPLC Column 30 97105-032130 - -
50 97105-052130 97105-053030 97105-054630
100 97105-102130 97105-103030 97105-104630
150 97105-152130 97105-153030 97105-154630
250 97105-252130 97105-253030 97105-254630
UNIGUARD Drop-in Guard
10 852-00 852-00 850-00
Cartridge Holder
Syncronis C8 pH Range
Reduces hydrophobic interactions allowing compounds to elute 0

Hydrophobicity
2 to 8 14
quicker from the column. Recommended for analytes with medium

hydrophobicity or when a less hydrophobic phase is required to obtain Low High
optimum retention. Pore Size
0 100 300
Carbon Load (%)
Less hydrophobic than Syncronis C18 0 11 25
Double endcapped for extra surface coverage Particle Size 1.7m, 3m, 5m USP L7
Uron herbicides
1000
Syncronis C8, 5m, 150 x 4.6mm
3 Mobile Phase A: Water
800 Mobile Phase B: Acetonitrile
4 Gradient: 35 to 60% B in 10 minutes
2 Temperature: 30C
mAU
6 Detection: 240nm
400 Analytes: 1. Tebuthiuron
1 2. Metoxuron
3. Monuron
200 4. Chlorotoluron
5. Diuron
6. Linuron
0
0 2 4 6 8 10 12 14 16
Minutes
Syncronis C8
1.7 UHPLC Column 50 97202-052130 - -
100 97202-102130 - -
3 HPLC Column 50 97203-052130 - -
100 - 97203-103030 97203-104630
150 - - 97203-154630
5 Drop-in Guard (4/pk) 10 97205-012101 97205-013001 97205-014001

HPLC Column 30 97205-032130 - -
50 97205-052130 97205-053030 97205-054630
100 97205-102130 97205-103030 97205-104630
150 97205-152130 97205-153030 97205-154630
250 - 97205-253030 97205-254630
UNIGUARD Drop-in Guard 10 852-00 852-00 850-00
Cartridge Holder
4-064
Syncronis aQ pH Range

Polar endcapped Syncronis aQ columns provide a controlled 0
Hydrophobicity
2 to 8 14
interaction mechanism that retains and resolves polar analytes

Low High
Pore Size
Stable in 100% aqueous mobile phase
0 100 300
Enhanced retention of polar compounds
Carbon Load (%)
0 19 25
In comparison to a conventionally endcapped C18, the Syncronis aQ polar end-capped C18 stationary
phase exhibits superior stability towards aqueous mobile phase. Syncronis aQ shows no degradation
in performance after 100 injections in a buffered 100% aqueous eluent.
Stability of Syncronis
DAD1 A, Sig=260,4 aQ in 100% aqueous mobile phase
Ref=360,100 (110809B\R2000001.D)
80 Syncronis aQ, 5m, 100 x 4.6mm

Mobile Phase: 50mM Aqueous K 2HPO 4 (pH 6)
Syncronis
60 Temperature: 30C
mAU
40
Detection: 260nm
Analytes: 1. Cytidine-5-diphosphate
20 2. Adenosine-5-triphosphate
3. Adenosine-5-diphosphate
4. Adenosine-5-monophosphate
0
0DAD1 A, Sig=260,4
1 Ref=360,100
2 (110809B\R2000085.D)
3 4 5 6 7 8
Minutes 100% aqueous inj 2
80
60
mAU
40
20
0 1 2 3 4 5 6 7 8
Minutes 100% aqueous inj 100
Syncronis aQ
1.7 UHPLC Column 50 97302-052130 - - 97302-054630
100 97302-102130 97302-103030 - -
3 HPLC Column 50 97303-052130 - - -
100 - 97303-103030 - 97303-104630
150 - - - 97303-154630
5 Drop-in Guard (4/pk) 10 97305-012101 97305-013001 97305-014001 97305-014001
50 97305-052130 97305-053030 - 97305-054630
100 97305-102130 - - 97305-104630
150 97305-152130 97305-153030 97305-154030 97305-154630
250 - 97305-253030 97305-254030 97305-254630
UNIGUARD Drop-in Guard 10 852-00 852-00 850-00 850-00
Cartridge Holder
Syncronis Phenyl
pH Range
Provides an alternative to Syncronis C18 and is particularly useful 0 2 to 8 14

Hydrophobicity
for retention of aromatic compounds
Low High
Pore Size
Alternative selectivity to Syncronis C18
Double endcapped for extra surface coverage 0 100 300
Carbon Load (%)
Highly inert towards basic compounds
Rigorously tested to ensure quality 0 11 25
Oxacillin sodium (USP)

140 Syncronis Phenyl, 5m, 300 x 4.0mm
Mobile Phase: Phosphate Buffer: MeCN:MeOH
120 (70:30:10)
Temperature: 25C
100 Flow Rate: 1.0mL/min (2mL/min in USP method)
80
mAU
60 Analytes: 1. Oxacillin Sodium (0.11mg/mL)
40
20
0
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Minutes
Syncronis Phenyl
1.7 UHPLC Column 50 97902-052130 - -
100 97902-102130 - -
3 HPLC Column 50 97903-052130 - -
100 - - 97903-104630
150 - - 97903-154630
5 Drop-in Guard (4/pk) 10 97905-012101 97905-013001 97905-014001
HPLC Column 50 - 97905-053030 97905-054630
100 - 97905-103030 97905-104630
150 97905-152130 - 97905-154630

250 - - 97905-254630
Cartridge Holder
4-066
Syncronis Amino

pH Range
Provides a versatile aminopropyl phase that gives excellent 0 2 to 8 14

Hydrophobicity
chromatographic properties in four modes: weak anion exchange,
reversed-phase, normal phase and HILIC Low High
Pore Size
Highly pure, high surface area silica 0 175 300

Carbon Load (%)
Double endcapped for extra surface coverage
Rigorously tested to ensure quality 0 4 25
Lactulose
Syncronis Amino 5m, 150 x 4.6mm
Mobile Phase: Water: MeCN (30:70)
2.00
Temperature: 35C
1.50 Detection: RI
Analytes: 1. Lactulose
uRIU
1.00
0.50
Syncronis
0.00
-0.25
0 1 2 3 4 5 6 7 8 9 10
Minutes
Syncronis Amino
1.7 UHPLC Column 50 97702-052130 - - -
100 97702-102130 - - -
3 HPLC Column 50 97703-052130 - - -
5 Drop-in Guard (4/pk) 10 97705-012101 97705-013001 97705-014001 97705-014001
HPLC Column 30 97705-032130 - - -
50 97705-052130 - - 97705-054630
150 97705-152130 97705-153030 97705-154030 97705-154630
250 - 97705-253030 97705-254030 97705-254630
Cartridge Holder
Syncronis Silica pH Range
Serves as a powerful and efficient tool for the chromatography 0

Hydrophobicity
2 to 8 14
of moderately polar organic compounds by normal phase chromatography

Low High
Pore Size
0 100 300
Excellent reproducibility for normal phase chromatography
Carbon Load (%)
0 25

Tocopherols
12
Syncronis Silica 5m, 150 x 4.6mm
10 Mobile Phase: Hexane +0.2% propan-2-ol (IPA)
Temperature: 40C
mAU
6 Analytes: a-tocopherol
b -tocopherol
g -tocopherol
4 d-tocopherol
0 5 10 15 20 25
Minutes
Syncronis Silica
1.7 UHPLC Column 50 97002-052130 - - -
100 97002-102130 - - -
3 HPLC Column 50 97003-052130 - - -
5 Drop-in Guard (4/pk) 10 97005-012101 97005-013001 97005-014001 97005-014001
HPLC Column 50 97005-052130 - - -
100 97005-102130 - - -
150 97005-152130 97005-153030 97005-154030 97005-154630
250 - 97005-253030 97005-254030 97005-254630

Cartridge Holder
4-068
Syncronis HILIC pH Range

Provides enhanced retention of polar and hydrophilic analytes 0 2 to 8 14
Hydrophobicity
Low High
Alternative selectivity to Syncronis C18 Pore Size
Improved sensitivity with MS detection
0 100 300
No need for ion-pair or derivatization
Carbon Load (%)
Outstanding peak shape and sensitivity
0 5 25
Highly pure, high surface area silica particles
Particle Size 1.7m, 3m, 5m
Neutral (uncharged), highly polar surface
Catecholamines Syncronis HILIC, 5m, 250 x 4.6mm

500 Mobile Phase: Water : acetonitrile : 200mM
1 ammonium formate (10.5 : 84.5 : 5)
400 Temperature: 40C
mAU
Analytes: 1. catechol
2 2. 5-HIAA
200
Syncronis
4 3. DOPAC
3 4. serotonin
100 5. tyrosine
5 6. dopamine
6 7 7. L-DOPA
0
0 5 10 15 20
Minutes
Syncronis HILIC
1.7 UHPLC Column 50 97502-052130 - 97502-054630
100 97502-102130 - -
3 HPLC Column 50 97503-052130 - -
100 - - 97503-104630
150 - - 97503-154630
5 Drop-in Guard (4/pk) 10 97505-012101 97505-013001 97505-014001
HPLC Column 50 97505-052130 - 97505-054630
100 97505-102130 - 97505-104630
150 97505-152130 97505-153030 97505-154630
250 - - 97505-254630
Cartridge Holder
Hypersil BDS and Hypersil

Classical HPLC Columns
Exceptionally reliable and reproducible
general purpose columns
Exceptionally reliable robust columns that guarantee you have the same results
time after time, even after changing your column. Chemistries available for a variety
of applications.
Excellent Reproducibility
Manufactured to the highest standards
Very Robust and Rugged

Rigorous quality control under certified processes
Long Column Lifetimes

Ideal for QA/QC labs
The Hypersil BDS/Classical web page contains the

latest news, applications and downloads for the
Hypersil BDS/Classical HPLC column range.
Visit www.thermoscientic.com/columns
4-070
Hypersil BDS

A good choice for robust, general purpose columns
Hypersil BDS
Particle Size (m) Format Length (mm) ID (mm) C18 C8 Phenyl CN
2.4 Drop-in Guard 10 2.1 28102-012101 28202-012101 - -
(4/pk) 4.0/4.6 28102-014001 28202-014001 - -
50 2.1 28102-052130 28202-052130 - -
4.6 28102-054630 - - -
100 2.1 28102-102130 28202-102130 - -
4.6 28102-104630 28202-104630 - -
150 4.6 - 28202-154630 - -
3 Drop-in Guard 10 2.1 28103-012101 28203-012101 - -
(4/pk) 3.0 28103-013001 28203-013001 - -
4.0/4.6 28103-014001 28203-014001 28903-014001 28803-014001
HPLC Column 30 2.1 28103-032130 - - -
50 2.1 28103-052130 28203-052130 - -
3.0 28103-053030 - - -
4.6 28103-054630 28203-054630 - -
100 2.1 28103-102130 - - -
Hypersil BDS
3.0 28103-103030 - - -
4.0 28103-104030 - - -
4.6 28103-104630 28203-104630 - -
150 2.1 28103-152130 - - -
3.0 28103-153030 28203-153030 - -
4.0 28103-154030 - - -
4.6 28103-154630 28203-154630 28903-154630 28803-154630
5 Drop-in Guard 10 2.1 28105-012101 28205-012101 - -
(4/pk) 3.0 28105-013001 28205-013001 - -
4.0/4.6 28105-014001 28205-014001 28905-014001 28805-014001
HPLC Column 50 2.1 28105-052130 28205-052130 - -
3.0 28105-053030 28205-053030 - -
4.6 28105-054630 28205-054630 - -
100 2.1 28105-102130 28205-102130 - -
3.0 28105-103030 - - -
4.0 28105-104030 - - -
4.6 28105-104630 28205-104630 - -
125 3.0 28105-123030 - - -
4.0 28105-124030 - - -
4.6 28105-124630 - - -
150 2.1 28105-152130 - - -
3.0 28105-153030 - - -
4.0 28105-154030 28205-154030 - -
4.6 28105-154630 28205-154630 28905-154630 28805-154630
250 2.1 28105-252130 - - -
3.0 28105-253030 - - -
4.0 28105-254030 28205-254030 28905-254030 -
4.6 28105-254630 28205-254630 28905-254630 28805-254630

UNIGUARD Guard 10 1.0 851-00
Cartridge Holder 2.1 852-00
3.0 852-00
4.0/4.6 850-00
Hypersil Classical
A global standard for many existing methods
Hypersil ODS
3 Drop-in Guard (4/pk) 10 2.1 30103-012101
3.0 30103-013001
4.0/4.6 30103-014001
HPLC Column 50 3.0 30103-053030
4.6 30103-054630
100 2.1 30103-102130
3.0 30103-103030
4.0 30103-104030
4.6 30103-104630
125 4.0 30103-124030
4.6 30103-124630
150 2.1 30103-152130
3.0 30103-153030
4.0 30103-154030
4.6 30103-154630
250 2.1 30103-252130
3.0 30103-253030
4.0 30103-254030
4.6 30103-254630
5 Drop-in Guard (4/pk) 10 2.1 30105-012101
3.0 30105-013001
4.0/4.6 30105-014001
HPLC Column 50 3.0 30105-053030
4.6 30105-054630
100 2.1 30105-102130
3.0 30105-103030
4.0 30105-104030
4.6 30105-104630
125 3.0 30105-123030
4.0 30105-124030
4.6 30105-124630
150 2.1 30105-152130
3.0 30105-153030
4.0 30105-154030
4.6 30105-154630
200 2.1 30105-202130
4.0 30105-204030
4.6 30105-204630
250 2.1 30105-252130
3.0 30105-253030
4.0 30105-254030
4.6 30105-254630
4-072
Hypersil ODS-2
3 Drop-in Guard (4/pk) 10 4.0/4.6 31603-014001
HPLC Column 50 4.6 31603-054630
100 4.0 31603-104030
4.6 31603-104630
150 4.6 31603-154630
5 Drop-in Guard (4/pk) 10 4.0/4.6 31605-014001
HPLC Column 50 4.6 31605-054630
100 4.6 31605-104630
150 4.6 31605-154630
250 4.0 31605-254030
4.6 31605-254630
Hypersil MOS (C8)

5 Drop-in Guard (4/pk) 10 4.0/4.6 30205-014001
HPLC Column 50 4.6 30205-054630
100 4.6 30205-104630
150 4.6 30205-154630
Hypersil Classical
250 4.0 30205-254030
4.6 30205-254630
Hypersil MOS-2 (C8)

5 Drop-in Guard (4/pk) 10 4.0/4.6 30305-014001
HPLC Column 150 4.6 30305-154630
250 4.0 30305-254030
4.6 30305-254630
Hypersil SAS (C1)

5 Drop-in Guard (4/pk) 10 4.0/4.6 30505-014001
HPLC Column 150 4.6 30505-154630
250 4.6 30505-254630
Hypersil Phenyl
5 Drop-in Guard (4/pk) 10 4.0/4.6 30905-014001
HPLC Column 50 4.6 30905-054630
150 4.6 30905-154630
250 4.0 30905-254030
4.6 30905-254630
Hypersil Phenyl-2
5 Drop-in Guard (4/pk) 10 4.0/4.6 31905-014001
HPLC Column 150 4.6 31905-154630
250 4.6 31905-254630
Hypersil CPS
3 Drop-in Guard (4/pk) 10 4.0/4.6 30803-014001
HPLC Column 150 4.6 30803-154630
5 Drop-in Guard (4/pk) 10 4.0/4.6 30805-014001
HPLC Column 150 4.6 30805-154630
250 4.0 30805-254030
4.6 30805-254630
Hypersil CPS-2
5 Drop-in Guard (4/pk) 10 4.6 31805-014001
HPLC Column 150 4.6 31805-254630
250 4.6 31805-254630
Hypersil APS-2
3 Drop-in Guard (4/pk) 10 2.1 30703-012101
4.6 30703-014001
HPLC Column 50 4.6 30703-054630
100 2.1 30703-102130
150 2.1 30703-152130
4.6 30703-154630
5 Drop-in Guard (4/pk) 10 3.0 30705-013001
4.0/4.6 30705-014001
HPLC Column 100 3.0 30705-103030
4.6 30705-104630
150 4.6 30705-154630
250 4.0 30705-254030
4.6 30705-254630
Hypersil Silica
3 Drop-in Guard (4/pk) 10 2.1 30003-012101

3.0 30003-013001
4.0/4.6 30003-014001
HPLC Column 50 2.1 30003-052130
4.6 30003-054630
150 4.6 30003-154630
5 Drop-in Guard (4/pk) 10 3.0 30005-013001
4.0/4.6 30005-014001
HPLC Column 50 4.6 30005-054630
100 4.6 30005-104630
150 4.6 30005-154630
250 4.0 30005-254030
4.6 30005-254630
4-074
Hypersil Classical continued

Hypersil SAX
5 Drop-in Guard (4/pk) 10 3.0 34105-013001
4.6 34105-014001
HPLC Column 250 3.0 34105-253030
4.6 34105-254630
Hypersil Green PAH

3 Guard Cartridge 10 2.1 31103-012101
3.0 31103-013001
4.6 31103-014001
HPLC Column 100 2.1 31103-102130
Hypersil Classical
3.0 31103-103030
4.6 31103-104630
HPLC Column 150 2.1 31103-152130
4.6 31103-154630
5 Guard Cartridge 10 4.6 31105-014001
HPLC Column 100 4.6 31105-104630
150 4.6 31105-154630
250 4.6 31105-254630
IonPac NS1 and NS2
Polymeric reversed-phase column ideal for the separation

of hydrophobic, ionizable compounds
Excellent resolution
Good peak shape
Ideal for separation of large molecules that carry localized charges, such as surfactants
Compatible with acids, bases, and solvent from pH 0 to 14
Can also be used for traditional polymeric reversed-phase applications
Utilize ion-pair chromatography for difficult separations
Thermo Scientific IonPac NS1-10m and NS1-5m columns are packed with a neutral, macroporous, high-surface-
area, ethylvinylbenzene polymer crosslinked with 55% divinylbenzene. This resin makes the NS1 resistant
to solvents, acids, and bases, and permits the use of eluent from pH 0 to 14. The Dionex IonPac NS1 column
is the column of choice for routine ion pair chromatography.
IonPac NS2 is a silica-based column for mobile-phase ion chromatography (MPIC) applications using eluents containing
trifluoroacetic acid (TFA), heptafluorobutyric acid (HFBA), or tetrabutylammonium borate (TBAB). It provides high
performance analysis for hydrophobic amines and hydrophobic acids using suppressed conductivity detection.
IonPac NS1 and NS2

Description Particle Size (m) Format Length (mm) 2.0mm ID 4.0mm ID
IonPac NS1 5 HPLC Column 150 039568
10 Guard Column 35 088763 039567
HPLC Column 250 088762 035321
IonPac NS2 5 Guard Column 35 088788
250 088786
4-076
HyperREZ XP

Polymer-based columns for carbohydrate analysis
Designed for the determination of carbohydrates, saccharides, 8m, 10m

organic acids, and alcohols
Efficient and reproducible monodisperse particles
Stable for long column lifetimes even at low pH and high temperatures
HyperREZ XP
HyperREZ XP Carbohydrate H+ 8 Guard Cartridge (2/pk) 5 3.0 69008-903027
Guard Column 50 7.7 69008-057726
HPLC Column 300 7.7 69008-307780
HyperREZ XP Carbohydrate Ca2+ 8 Guard Cartridge (2/pk) 5 3.0 69208-903027
Guard Column 50 7.7 69208-057726
HPLC Column 300 7.7 69208-307780
HyperREZ XP Carbohydrate Pb2+ 8 Guard Cartridge (2/pk) 5 3.0 69108-903027
HyperREZ
Guard Column 50 7.7 69108-057726
HPLC Column 300 7.7 69108-307780
HyperREZ XP Carbohydrate Na+ 10 Guard Cartridge (2/pk) 5 3.0 69310-903027
Guard Column 50 7.7 69310-057726
HPLC Column 300 7.7 69310-307780
HyperREZ XP Organic Acids 8 Guard Cartridge (2/pk) 5 3.0 69008-903027
HPLC Column 100 7.7 69608-107780
HyperREZ XP Sugar Alcohols 8 Guard Cartridge (2/pk) 5 3.0 69208-903027
HPLC Column 250 4.0 69708-254080

HyperRez Guard Cartridge Holder 5 3 60002-354
Retention Times of Common Saccharides (min)

HyperREZ Carbohydrate H+ 300 x 7.7mm
Saccharide H+ Ca2+ Pb2+ HyperREZ Carbohydrate Ca2+ 300 x 7.7mm
Adonitol 11.5 14.9 20.4 HyperREZ Carbohydrate Pb2+ 300 x 7.7mm
Mobile Phase: H2O
Arabinose 11.4 13.6 19.4
Erythritol 12.7 15.6 20.3 Detection: RI
Temperature: 75C (H +)
Fructose 10.6 13.5 19.3 85C (Ca 2+)
Fucose 12.2 13.7 17.1 80C (Pb )
2+
Note: partial hydrolysis may occur with some

Galactose 1.07 12.2 15.6 saccharides using H +.
Glucose 9.9 11.1 13.9
Glycerol 14.1 16.1 19.5
Lactose 8.6 9.7 12.8
Maltose 8.4 9.5 12.5
Maltotriose 7.7 8.7 11.9
Mannitol 11.0 17.3 28.9
Mannose 1.5 12.5 16.7
Raffinose 8.2 8.6 11.4
Sorbitol 11.1 20.7 N/A
Sucrose 9.8 9.4 11.9
Xylose 10.6 12.0 15.0
LC Accessories
Excellence in LC accessories providing
optimal performance
Thermo Scientific offers a wide range of accessories to suit your LC needs,
ensuring confidence in your analysis and delivery of exceptionally reproducible
data and reliable chromatography.
Viper Fingertight Fitting

Providing zero dead volume fingertight
connections up to 1,250 bar
Rheodyne Sample Injectors

Biocompatible and stainless steel versions
for highly accurate and precise injections
LC Syringes
Supporting manual and automated injections
Learn more about LC Accessories.

Visit www.thermoscientific.com/columns
4-078
Viper Fingertight Fittings
Provides ease of use and dead-volume free plumbing of every conventional
HPLC and UHPLC system
Provides zero-dead volume fingertight connections

Supports operating pressures up to 1,500 bar (22,000 psi)
Available in different lengths: 65mm and from 150 to 950mm in 100mm steps
Available in different inner diameters: 0.1mm (0.004in), 0.13mm (0.005in) or 0.18mm (0.007in)
Easy to use due to stainless steel or biocompatible MP35N capillaries (1/32in OD) and
fingertight design
Works with virtually any valve and column from any manufacturer
Fits narrow connections such as 10-port valves and enables mixed use with different designs

The Thermo Scientific Viper fingertight fitting valves, and columns quickly and easily without to reduce the dead volume of any fluidic
system provides ease of use and dead-volume tools is simple with the Viper system. connection to zero. The Viper system unifies
free plumbing of every conventional HPLC and Extra column volumes in HPLC have the most robust performance, ease of use, acceptable
modern UHPLC system. Together with flexible detrimental effects on the separation lifetime, and universal compatibility with
stainless steel (SST) or biocompatible MP35N efficiency of an LC system and must be virtually all different valves and columns for
capillaries, it opens a new dimension in liquid minimized. Conventional fittings tightened by HPLC system users. All Thermo Scientific
chromatography. The Viper system improves hand or using tools have considerable Dionex UltiMate 3000 XRS, RS, BioRS and SD
chromatographic results, independent of drawbacks which can compromise efficiency. systems are equipped with Viper fingertight
various different connection geometries and The Viper fitting system overcomes these fitting system as a standard.
system backpressures. Connecting LC modules, drawbacks by design, working without ferrules
Viper Fingertight Fitting Systems - SST

Length (mm) 0.1mm ID 0.13mm ID 0.18mm ID
65 6040.2207 6040.2307 6040.2357
150 6040.2215 6040.2315 6040.2360
250 6040.2225 6040.2325 6040.2385
350 6040.2235 6040.2335 6040.2375
450 6040.2245 6040.2345 6040.2365
550 6040.2255 6040.2305 6040.2355
650 6040.2265 6040.2310 6040.2395
750 - 6040.2320 6040.2370
850 - 6040.2330 6040.2380
950 - 6040.2340 6040.2390
Viper Fingertight Fitting Systems - Biocompatible

Length (mm) 0.065mm ID, PEEK 0.090mm ID, PEEK 0.1mm ID, MP35N 0.13mm ID, MP35N 0.13mm ID, PEEK 0.18mm ID, MP35N
65 - 6041.9075* 6042.2306 - - -
150 6041.5615 - 6042.2320 - 6041.5616 6042.2315
250 6041.5625 6041.9025 6042.2330 - - 6042.2327
350 - - 6042.2340 - - 6042.2337
450 - - 6042.2350 - - 6042.2365
550 - 6042.2360 - - 6042.2355
650 6041.5665 - 6042.2370 6042.2363 - 6042.2380
750 6041.5675 - 6042.2390 6042.2373 - 6042.2375
850 6041.5685 - - - - -
950 - - 6042.2395 - 6042.2385
* 75mm length
Viper Accessories
Description Cat. No. Quantity
Plug, Biocompatible 6040.2303 1 Each
Union, SST 6040.2304 1 Each
Calibration Tool 6040.2312 1 Each
Inline Filter 6036.1045 1 Each
4-080
Viper Fingertight Fitting Kits for UltiMate 3000 Systems

Viper Fingertight Fitting Kits for UltiMate 3000 Systems
Description SD Systems RS Systems
Viper Capillary Kit for ISO, LPG or DGP pumps 6040.2302 6040.2301
Viper Capillary Kit for HPG pumps 6040.2309 6040.2308
Viper Capillary Kit for biocompatible RSLC systems 6841.2301
Online SPE Solution Kit for x2 Dual Standard or RSLC systems 6040.2802 6040.2801
Tandem Operation Solution Kit for x2 Dual Standard or RSLC systems 6040.2804 6040.2803
Application Switching Solution Kit for x2 Dual Standard or RSLC systems 6040.2806 6040.2805
Parallel Setup Solution Kit for x2 Dual Standard or RSLC systems 6040.2810 6040.2809
Inverse Gradient Kit for x2 Dual Standard or RSLC systems 6040.2819 6040.2820
Automated Method Scouting Solution Kit for Standard or RSLC systems 6040.2808 6040.2807
MS Connection Kit for MS-frontends with WPS autosampler, excluding UV detection 6720.0355 6720.0370
MS Connection Kit for MS-frontends with WPS autosampler, including UV detection 6720.0365 6720.0375
MS Connection Kit for MS-frontends with HPG-RS pump and OAS autosampler excluding UV detection 6720.0372
MS Connection Kit for MS-frontends with HPG-RS pump and OAS autosampler including UV detection 6720.0377
Description XRS Systems

Viper Capillary Kit for standalone system with with WPS autosampler 6043.2301
Viper Capillary Kit for standalone system with with OAS autosampler 6845.2301A
MS Connection Kit for MS-frontends with WPS autosampler excluding UV detection 6720.0380
MS Connection Kit for MS-frontends with WPS autosampler including UV detection 6720.0385
MS Connection Kit for MS-frontends with LPG-XRS pump and OAS autosampler excluding UV detection 6720.0372
MS Connection Kit for MS-frontends with LPG-XRS pump and OAS autosampler including UV detection 6720.0377
Javelin Direct-Connection Column Filters
One-piece filter protects HPLC systems
Direct-connection design for maximum efficiency

Replace entire disposable filter unit for easy changes
Recommended for use as dedicated filters for a column rather than the HPLC system
1/16in CPI tip attaches directly to HPLC column inlet without tubing or wrenches
0.5m porosity
Javelin Direct-Connection Column Filter

Description 2.1mm ID 3.0mm ID 4.0/4.6mm ID Quantity
Javelin Column Filter 88200 88700 88400 4 Pack
ColumnSaver Precolumn Filters

Filter mesh size 2m
ColumnSaver Precolumn Filters

Filter Mesh Size (m) Cat. No Quantity
2 60140-412 10 Pack
UNIFILTER Direct-Connection HPLC Filter Systems

Quickly replaced for minimal down time
Replaceable 0.5m drop-in filter enhances column lifetime and improved performance
Holder attached directly to the inlet of your analytical system for maximum convenience
UNIFILTER Direct-Connection HPLC Filter Systems

Description 2.1/3.0mm ID 4.0/4.6mm ID Quantity
UNIFILTER Direct Connection Holder 27002 27000 1 Each
Replacement Filter, 0.5m 22017 22155 5 Pack
Replacement Tip, CPI, Standard 850-RT 850-RT 1 Each
Replacement Tip, Waters End-fitting 850-WT 850-WT 1 Each
UHPLC Filter
Column protection for Hypersil GOLD 1.9m and Syncronis 1.7m columns
without compromising performance
Low volume filter cartridge design

Maintain peak shape
Minimal efficiency loss through dispersion
UHPLC Filter
UHPLC Direct Connect Filter Holder 27006 1 Each
2.1mm ID Replacement Filter Cartridge, 0.2m 22180 5 Pack
1.0mm ID Replacement Filter Cartridge, 0.2m 22185 5 Pack
4-082
PTFE One-Piece Column Connector

Excellent for high-throughput screening and quick connection
Fingertight, leak-free connection of analytical and guard columns with 10-32 threads
Minimizes dead volume
Inert and biocompatible material
PEEK One-Piece Column Connector

One Piece Coupler 60170-370 1 Each
Solvent Filters
HPLC Filters
Efficient draw
100% PTFE polymer, including 2m filters

Built-in helium sparge port and frit
Solvent Filters
Type For Use with Cat. No. Quantity
Stainless Steel Fit 1/16in OD tube to 1/8in OD plastic tubing A-302 1 Each
Stainless Steel Fit to 1/8in OD plastic tubing using 1/8in PP nut A-302A 1 Each
Bottom-of-the-Bottle 3/16in OD plastic tubing A-436 1 Each
Bottom-of-the-Bottle 1/8in OD tubing A-437 1 Each
PEEKsil Capillary Tubing
Excellent chemical compatibility and very low carryover
Precision-bore fused silica tubing coated with 1/16in OD PEEK covering

Usable in most standard chromatography systems
Withstands high pressures
Smooth internal surface for excellent flow characteristics
Tubing is stiff: not recommended for uses requiring tubing bends
Precut lengths only: cutting in the lab may damage tubing
PEEKsil Capillary Tubing

ID (in) Length (cm) Cat. No. Quantity
0.002 10 60182-500 5 Pack Applications:
20 60182-501 5 Pack HPLC
50 60182-502 2 Pack LC-MS
0.004 10 60182-503 5 Pack
20 60182-504 5 Pack
50 60182-505 2 Pack
0.007 10 60182-506 5 Pack
20 60182-507 5 Pack
50 60182-508 2 Pack
PEEK Sleeves for Fused Silica Capillary Tubing

Withstands high pressures
1/16in OD PEEK Sleeves for Fused Silica Capillary Tubing

ID (in) Color Cat. No. Quantity
0.008 Yellow F-227 1 Each
0.010 Blue F-228 1 Each
0.012 Natural F-229 1 Each
0.015 Orange F-230 1 Each

4-084
316 Stainless Steel Capillary Tubing

Cleaned, polished, passivated and ready-to-use
Suitable for ultra high pressure applications

Wide chemical compatibility
Prefinished, square, burr-free ends and interiors to minimize dead volume connections
Not recommended for biological samples
Rough internal surface may lead to sample carryover
316 Stainless Steel Capillary Tubing

ID (in) Length (cm) Color Cat. No. Quantity
1/16in OD Precut Tubing
0.005 5 Red U-152 1 Each
10 Red U-153 1 Each
20 Red U-154 1 Each
30 Red U-155 1 Each
50 Red U-156 1 Each
100 Red U-157 1 Each
U-126
LC Tubing
0.007 5 Black 1 Each
10 Black U-127 1 Each
0.010 5 Blue U-111 1 Each
10 Blue U-112 1 Each
1/32in OD Precut Tubing with 1/16in Sleeves
0.005 10.5 Red 30011-FLEX 1 Each
15 Red 30015-FLEX 1 Each
0.007 10.5 Yellow 39011-FLEX 1 Each
15 Yellow 39015-FLEX 1 Each
1/16in 316 Stainless Steel Tubing, 5-Foot Coil

ID (in) Cat. No. Quantity
0.005 U-158 1 Each
0.007 U-108 1 Each
0.010 U-106 1 Each
0.020 U-105 1 Each
0.030 U-107 1 Each
0.040 U-144 1 Each
Terry Tool Tubing Cutters

1/16in stainless steel tubing 60182-509 1 Each
1/8in stainless steel tubing 60182-510 1 Each
PEEK Capillary Tubing
Pre-cut and color-coded for easy identification and use
Broad chemical compatibility

Biocompatible
Easily cut to desired length
Appropriate for many HPLC applications
Resistant to most organic solvents, except for nitric acid, sulfuric acid, dichloromethane, THF and DMSO
1/16in OD Precut PEEK Tubing

ID (in) Length (cm) Color Cat. No. Quantity
0.003 5 Natural 37003-5 1 Each
10 Natural 37003-10 1 Each
0.005 5 Red 37005-5 1 Each
10 Red 37005-10 1 Each
20 Red 37005-20 1 Each
30 Red 37005-30 1 Each
50 Red 37005-50 1 Each
0.007 5 Yellow 37007-5 1 Each
10 Yellow 37007-10 1 Each
0.010 5 Blue 37010-5 1 Each
10 Blue 37010-10 1 Each
20 Blue 37010-20 1 Each
30 Blue 37010-30 1 Each
50 Blue 37010-50 1 Each
0.020 5 Orange 37020-5 1 Each
10 Orange 37020-10 1 Each
1/16in OD PEEK Tubing, 5-Foot Coil

ID (in) Cat. No. Quantity
0.003 37003 1 Each
0.005 37005 1 Each
0.007 37007 1 Each
0.010 37010 1 Each
0.020 37020 1 Each
0.030 37030 1 Each
0.040 37040 1 Each
Polymer Tubing Cutter

Polymeric Tubing Cutter A-327 1 Each
Replacement blades A-328 5 Pack
4-086
High Pressure Stainless Steel Nuts and Ferrules

Accommodate a wide range of configurations
Designed for 10-32 port configurations

Burr and contaminant free
High Pressure Stainless Steel Nuts and Ferrules

Type Nut Quantity Description Replacement Ferrule Quantity
Cat. No. Cat. No.
Replacement PEEK
10-32 thread nut with ferrule F-190 1 Each F-192x 10 Pack
Ferrules
Universal Ferrules,
Male hex nut U-400x 10 Pack U-401x 10 Pack
0.625in
Valco ferrules,
Valco male hex nut, 10-32 thread U-320x 10 Pack U-321x 10 Pack
0.625in
Universal Ferrules,
Male hex nut, Waters compatible U-410x 10 Pack U-401x 10 Pack
0.625in
LC Fittings
Reducing Union for Preparative Columns
Connects 30 to 50mm ID preparative columns to 1/16in tubing
Stainless steel construction

1.0mm bore
Without frit
Reducing Union for Preparative Column

1/8in to 1/16in Reducing Union for Preparative Column 60182-357 1 Each
PEEK Fingertight Fittings

Machined for reliability and ease of use
Resist cracking, breaking, thread stripping and leaking in both low and high pressure applications
Biocompatible for a broad range of applications
PEEK Fingertight Fittings

Type Cat. No. Quantity
One-Piece Fingertight Fitting, 1/16in, 0.37in head F-120x 10 Pack
One-Piece Long Fingertight Fitting, 1/16in, 0.37in head F-130x 10 Pack
One-Piece PEEK Fingertight Fitting, 1/32in, 0.25in head M-645 1 Each
Two-Piece Fingertight Wing Nut with Ferrule, 1/16in F-300x 10 Pack
Replacement PEEK Ferrules F-142x 10 Pack
Column End Plug, 1/16in, 10-32 coned, Delrin, Red U-467R 1 Each
Column End Plug, 1/16in, 10-32 coned, Delrin, Black U-467BLK 1 Each
Stainless Steel Unions, Tees and Crosses
Well-suited to high-pressure applications
Absolute zero or low dead volume formats

Includes two stainless steel nuts and ferrules
Stainless Steel Unions, Tees and Crosses

Description Through Hole (in) Swept Volume (L) Cat. No. Quantity
Union, stainless steel, Upchurch Scientific/Parker fittings
0.010 0.025 U-435 1 Each
compatible, includes 2 stainless steel nuts and ferrules

0.020 0.134 U-402 1 Each

0.050 0.836 U-437 1 Each

0.062 ~0.0 U-438 1 Each
Union, stainless steel, Waters fittings compatible,

0.020 0.129 U-412 1 Each
includes 2 stainless steel nuts and ferrules
Union, stainless steel, Valco fittings compatible,

0.020 0.103 U-322 1 Each
includes 2 stainless steel nuts and ferrules
Tee, stainless steel, 10-32 fittings

0.020 0.57 U-428 1 Each
for use with 1/16in OD tubing
Cross, stainless steel, 10-32 fittings

0.020 0.72 U-430 1 Each
for use with 1/16in OD tubing
PEEK Unions, Tees and Crosses

Well-suited to high pressure applications
Absolute zero or low dead volume formats

Biocompatible
PEEK Unions, Tees and Crosses

Description Through Hole (in) Swept Volume (L) Cat. No. Quantity
Union, PEEK polymer, includes two PEEK 2-piece fittings 0.010 0.070 P-742 1 Each
Union, PEEK polymer, includes two PEEK 2-piece fittings 0.020 0.28 P-704 1 Each
Tee, PEEK, 10-32 fittings for use with 1/16in OD tubing,

0.020 0.57 P-727 1 Each
includes three 10-32 PEEK double-winged nuts
PEEK, 10-32 fittings for use with 1/16in OD tubing, includes

0.020 0.72 P-729 1 Each
four 10-32 PEEK double-winged nuts
4-088
Rheodyne 7725 and 7725i Sample Injectors

Allow continuous flow between the load and inject positions to protect
against pressure shock
Stainless steel construction

Make-Before-Break (MBB) design
Can use partial filling for zero sample waste or complete filling for better reproducibility
Inject 1L to 5mL with high accuracy and precision
7725i features a position sensing switch for a reproducible start signal

Model Mode Features Cat. No. Quantity
7725 Dual Continuous flow 7725 1 Each
7725i Continuous flow, position
Dual 7725i 1 Each
sensing switch
Stainless Steel Sample Loops
LC Injectors
Description Volume ID (mm / in) Cat. No. Quantity
Sample loops for 5L 0.18 / 0.007 7755-020 1 Each
7725 and 7725i 10L 0.30 / 0.012 7755-021 1 Each
injectors
20L 0.30 / 0.012 7755-022 1 Each
50L 0.51 / 0.020 7755-023 1 Each
RheBuild Kits
For Use with Rheodyne Models Cat. No. Quantity
7725/7725i 7725-999 1 Each
Rheodyne Stators
7725 7725-010 1 Each
Allow continuous flow between the load and inject positions to protect against
pressure shock
Biocompatible PEEK construction

Make-Before-Break (MBB) design
Inject 1L to 5mL with high accuracy and precision
9725i features a position sensing switch for a reproducible start signal

9725i Continuous flow, position sensing
Dual 9725i 1 Each
switch
PEEK Sample Loops

Sample loops for 2L Internal 7755-015 1 Each
9725 and 9725i 5L 0.18 / 0.007 9055-020 1 Each
injectors
10L 0.25 / 0.010 9055-021 1 Each
20L 0.25 / 0.010 9055-022 1 Each
50L 0.51 / 0.020 9055-023 1 Each
100L 0.51 / 0.020 9055-024 1 Each
200L 0.51 / 0.020 9055-025 1 Each
500L 0.76 / 0.030 9055-026 1 Each
1mL 0.76 / 0.030 9055-027 1 Each
5mL 0.76 / 0.030 9055-029 1 Each
Rheodyne Suction Needle Adapter

For Use with Cat. No. Quantity
Rheodyne Injector Models 9725 and 9725i 9125-076 1 Each
4-090
Rheodyne 8125 Low-Dispersion Microscale Injector

Designed for use with 1 and 2mm ID HPLC columns
Position sensing switch provides reproducible start signal
Suitable for use with 5 to 50L sample loops
Rheodyne 8125 Low-Dispersion Microscale Injector


Sample loops for 5L 0.20 / 0.008 8020 1 Each
8125 injectors 10L 0.20 / 0.008 8021 1 Each
20L 0.25 / 0.010 8022 1 Each
50L 0.30 / 0.012 8023 1 Each
LC Injectors
RheBuild Kits
8125 8125-999 1 Each
Rheodyne Replacement Rotor Seals for Injectors

Vespel Seals
8125/8126 8125-038 1 Each
Tefzel Seals
8125 8125-097 1 Each
Rheodyne Stators
8125/8126 8125-098 1 Each
Rheodyne Stator Face Assemblies

8125 8125-074 1 Each
Rheodyne 7010 Sample Injector
Single-mode sample injector designed for the complete filling method

Model Mode Features Cat. No. Quantity Compatible with:
7010 Single Complete filling method 7010 1 Each Sample loop sizes 5L to 20mL
Rheodyne Ports for Rheodyne Injectors

For Use with Rheodyne Model Cat. No. Quantity
7010 Filler Port, Stainless Steel 7012 1 Each

Sample loops for 5L 0.18 / 0.007 7020 1 Each
7010 and 7125 10L 0.30 / 0.012 7021 1 Each
injectors
20L 0.30 / 0.012 7022 1 Each
50L 0.51 / 0.020 7023 1 Each
100L 0.51 / 0.020 7024 1 Each
200L 0.76 / 0.030 7025 1 Each
500L 0.76 / 0.030 7026 1 Each
1mL 0.76 / 0.030 7027 1 Each
5mL 1.0 / 0.040 7029 1 Each
RheBuild Kits
7010/7000 7010-999 1 Each


Vespel Seals
7010 7010-039 1 Each
Tefzel Seals
7010 7010-071 1 Each
4-092

Single-mode sample injector designed for the complete filling method
Compatible with sample loop sizes 5L to 10mL

PEEK stator
Position sensing switch provides a reproducible start signal

9010 Continuous flow, position sensing
Single 9010 1 Each
switch
Rheodyne Ports for Rheodyne Injectors

For Use with Rheodyne Model Cat. No. Quantity
9010 Filler Port, PEEK 9012 1 Each
9010 Needle Port, PEEK 9013 1 Each
PEEK Sample Loops
LC Injectors
Sample loops for 2L Internal 7755-015 1 Each
9010 injectors 5L 0.18 / 0.007 9055-020 1 Each
10L 0.25 / 0.010 9055-021 1 Each
20L 0.25 / 0.010 9055-022 1 Each
50L 0.51 / 0.020 9055-023 1 Each
100L 0.51 / 0.020 9055-024 1 Each
200L 0.51 / 0.020 9055-025 1 Each
500L 0.76 / 0.030 9055-026 1 Each
1mL 0.76 / 0.030 9055-027 1 Each
5mL 0.76 / 0.030 9055-029 1 Each

Tefzel Seals
9010 9010-051 1 Each
Rheodyne Stators
9010 9125-043 1 Each
Rheodyne Stator Face Assemblies

9125 8125-094 1 Each
RheFlex High Pressure Fittings
Precision machined from 316 stainless steel
RheFlex High Pressure Fittings

Short Fittings Set 6000-109 5 Pack
Short Fittings Set 6000-209 10 Pack
Long Fittings Set 6000-111 5 Pack
Long Fittings Set 6000-211 10 Pack
Extra Long Fittings Set 6000-162 5 Pack
Extra Long Fittings Set 6000-262 10 Pack
1/16in Ferrule 6000-110 5 Pack
1/16in Ferrule 6000-210 10 Pack
RheFlex Two-Piece PEEK Fittings

Provide inert, metal-free connections
Slotted backside of the ferrule is squeezed down onto the tube by the
mating conical surface of the nut
May be used on 1/16in metal or plastic tubing reliably up to 5000psi
Reusable ferrule and nut
RheFlex Two-Piece PEEK Fittings

Fitting set, standard length 6000-054 5 Pack
Fitting set, short 6000-055 5 Pack
Fitting set, X-long 6000-066 1 Each
Replacement ferrules 6000-051 5 Pack
4-094
Cheminert Model C2 Microbore Injector

Can be used as an injector or switching valve
1/16in fittings
0.010in ports
Available in 6-port or 10-port configurations
Available with manual or microelectric actuator
Cheminert Model C2 Microbore Injector

Description Sample Volume Cat. No. Quantity
Model C2 injector, N60 stainless stator, 5Lloop, manual 6 ports C2-1006 1 Each
10 ports C2-1000 1 Each
Model C2 injector, N60 stainless stator, 5L loop, 6 ports C2-1006EH 1 Each
microelectric actuator 10 ports C2-1000EP 1 Each
Sample injector loops, stainless steel 5L CSL5 1 Each
10L CSL10 1 Each
20L CSL20 1 Each
50L CSL50 1 Each
LC Injectors
100L CSL100 1 Each
Model C4 injector, PAEK stator, 5L loop,
6 ports C2-1346EH 1 Each
microelectric actuator
Sample injector loops, PAEK 5L CZSL5PK 1 Each
10L CZSL10PK 1 Each
50L CZSL50PK 1 Each
Valco Injector Model C6W

Description Volume Cat. No. Quantity
Model C6W injector, six 0.016in ports, manual 20L loop C6W 1 Each
Model EPC6W injector, six 0.016in ports, microelectric actuator 20L loop EPC6W 1 Each
Replacement rotor SSAC6W 1 Each
Sample injector loop, stainless steel 2L SL2CW 1 Each
Valco Accessories
Description Volume Cat. No. Quantity
Valco syringe ports 22 ga. needles; 1/16in fittings VISF-1 1 Each
Valco syringe ports 22 ga. 2in needles VISF-2 1 Each
Valco Nuts and Ferrules 1/16in standard nut ZN1-10 10 Pack
Valco Nuts and Ferrules 1/16in long nut LZN1-10 10 Pack
Valco Nuts and Ferrules 1/16in SS ferrule ZF1-10 10 Pack
LC Syringes for Manual Injection valves
Easy, accurate and reproducible manual injection
Square tip to prevent damage to the injector

Wide range of volumes
Precision made from borosilicate glass and stainless steel
Robust design and easy-to-read markings
Removable Needle, Gas Tight Syringes for Rheodyne / Valco Injectors

Volume (L) Length (mm) Gauge Tip Style Cat. No. Quantity Replacement Needle Quantity
Cat. No.
10 50 22 90 Blunt End 365DLG21 1 Each 365RNL15 5 Pack
25 50 22 90 Blunt End 365FLG31 1 Each 365RNL25 5 Pack
50 50 22 90 Blunt End 365GLG41 1 Each 365RNL25 5 Pack
100 50 22 90 Blunt End 365HLG51 1 Each 365RNL25 5 Pack
500 50 22 90 Blunt End 365JLG71 1 Each 365RNL25 5 Pack
Fixed Needle, Gas Tight Syringes for Rheodyne / Valco Injectors

Volume (L) Length (mm) Gauge Tip Style Cat. No. Quantity
10 50 22 90 Blunt End 365DL263 1 Each
25 50 22 90 Blunt End 365F6315 1 Each
50 50 22 90 Blunt End 365G6316 1 Each
100 50 22 90 Blunt End 365H6317 1 Each
500 50 22 90 Blunt End 365J6319 1 Each
Fixed Needle Syringes for Rheodyne / Valco Injectors

5 50 22 90 Blunt End 365CL221 1 Each
10 50 22 90 Blunt End 365DL231 1 Each
25 50 22 90 Blunt End 365FL241 1 Each
50 50 22 90 Blunt End 365GL251 1 Each
100 50 22 90 Blunt End 365HL261 1 Each
250 50 22 90 Blunt End 365IL271 1 Each

500 50 22 90 Blunt End 365JL281 1 Each
4-096
HPLC Syringes

Instrument specific syringes
Luer-Lok syringes ideal for priming
Macro volume sampling syringes suitable for liquid or gas samples
Removable Needle Syringes for Thermo Scientific Instruments

Volume (L) Length (mm) Gauge Instrument Cat. No. Quantity
250 50 22 LCQ 365ILT21 1 Each
500 50 22 LCQ 365JLT41 1 Each
250 AS1000, AS3000 365ILT91 1 Each
500 AS1000, AS3000 365JLT61 1 Each
2500 AS3000, AS3500 365LLT81 1 Each
Mass Spectrometry Replacement ESI Probe Needles

Instrument Cat. No. Quantity
LCQ, XP, DECA, Advantage 365RNLT1 1 Each
LCQ MS 365RNLT2 1 Each
LC Syringes
LCQ XSQ 365RNLT3 1 Each
Fixed Needle Syringes for CTC Instruments

10 50 22 365DL991 1 Each
25 50 22 365FL715 1 Each
50 50 22 365GL810 1 Each
100 50 22 365HL331 1 Each
PTFE Luer-Lok Syringes

Volume (mL) Cat. No. Quantity Replacement Needle Quantity
Cat. No.
1 365KL531 1 Each 365RNL22 2 Pack
2.5 365LL541 1 Each 365RNL22 2 Pack
5 365ML551 1 Each 365RNL22 2 Pack
10 365NL561 1 Each 365RNL22 2 Pack
25 365PL571 1 Each 365RNL22 2 Pack
Syringes for Macro Volume Sampling

Volume (mL) Length (mm) Gauge Tip Style Cat. No. Quantity
1 50 22s Bevel 365K3051 1 Each
2.5 50 22s Bevel 365LL375 1 Each
5 50 22s Bevel 365M5212 1 Each
10 50 22s Bevel 365N5214 1 Each
HOT POCKET and COOL POCKET Column Temperature Controllers
Wrap-around column temperature control systems
Easy to install and use with a variety of column lengths

Dual display of both actual and set point temperature
HOT POCKET range from just above ambient to 85C
COOL POCKET range from 5C to 55C
Explore sample selectivity and stability on both sides of ambient
Product Specifications
HOT POCKET COOL POCKET
Operating Range 5C above ambient to 85C 5C to 55C
Display Dual LED displays of actual and set point temperatures in C
Temperature Accuracy 2C over entire range 2C over entire range
Temperature Repeatability 1C 1C
Temperature Stability 0.1C 0.1C
Time to Stabilization (from ambient) 85C in less than 30 minutes 55C in 25 minutes, 5C in 20 minutes
With Eluent Pre-heater Without Eluent Pre-heater
80C 80C
oven4 oven3
0 1 2 3 4 5 MIN 0 1 2 3 4 5 MIN
Effect of eluent pre-heater on efficiency

Data courtesy of Dr. Richard F. Myer, Quantitative Technologies, Inc., Whitehouse, NJ
HOT POCKET Column Heaters, Eluent Preheater/Precooler and COOL POCKET Chiller
HOT POCKET Column Heater 92016 1 Each
HOT POCKET Column Heater short version 92016-150 1 Each
COOL POCKET Column Chiller 92017 1 Each
Eluent Preheater/Precooler 92018 1 Each
4-098
H2N
O OH
-O O -O
N+
O- -O O
N+ N+
N+ O NH2
O O O
S
OH O
OH O
H2N O
O- OH
S -O
N+ N+
NH2
H2N

O
O -O O O
OH
-O O- N+ O
N+ N+ H
N
-O O
OH NH2
O-
O O -O
N+ N+
N+ NH2 O
H
N
O O OH
-O O-
NH2
N+ N+
O O
-O O
N+ O
H
N
OH
LC Reagents -O
N+ N+
O-
NH2
O O
Providing the selectivity needed for

high-quality separation of charged
compounds
LC Reagents
Control selectivity, resolve complex ionic mixtures and improve peak symmetry
with the use of our LC reagents.
By Using the Correct Reagent Types:

Reagent You Achieve:
Increased or decreased retention, permitting High purity pre-column derivatization reagents
controlled selectivity
Hydrolysis reagents
Resolution of complex ionic mixtures without using
ion exchange columns HPLC ion pair reagents
Improved peak symmetry Derivatization and visualization reagents

Amino acid detection reagents
Peptide standards
The LC Reagents web page contains the

latest news, applications and downloads
for the LC Reagents HPLC column range.
Visit www.thermoscientic.com/columns
LC Reagents
Technical Resources
O
O OH
O S O O
N N
+ +
N N TNBSA N S O
O O MW 293.17 Cl
+
N
O O
O
OH
Ninhydrin
MW 178.14
OH
visit www.thermoscientific.com/chromexpert
to access the following information:
Derivatization and Visualization Reagents for HPLC

Developments in Amino Acid Analysis
Hydrolysis
Methods for Fractionation of Peptide Mixtures
www.
thermoscientific.com/chromexpert
www.
/chromexpert
www.
/chromexpert
4-100
HPLC Ion Pair Reagents

Heptafluorobutyric Acid
Ion-pair reagent for the reversed-phase HPLC separation of proteins and peptides
Typical purity is 99.7% by GC; <0.1% water

Sequencing reagent for classical and automated Edman degradation of peptides and proteins
Density: 1.645
B.P. 120C
Packaged under nitrogen in amber glass ampules or bottles
Clear, colorless liquid
Heptafluorobutyric Acid
Description Quantity Cat. No. Quantity
Heptafluorobutyric Acid, Sequencing Grade 100mL X TS-25003 1 Each
Heptafluorobutyric Acid, HPLC Grade 10 x 1mL ampules TS-53104 1 Pack
X in the ordering table indicates that hazardous shipping charges apply.
LC Reagents
Triethylamine (TEA) 2(a);0.000M TEA 2(b);0.010M TEA 2(c);0.020M TEA
Ideal for HPLC separation and analysis of peptides
2,4-dimethylaniline
Triethylamine is an ion-pairing reagent that alters selectivity in reversed-phase
2,4-dimethylaniline
HPLC separations. By pairing with peptides, it effectively sharpens peaks, resulting
2,4-dimethylaniline
in improved peak resolution.

99.5% triethylamine purity, allowing sensitive peptide detection at low UV
wavelengths in reverse-phase HPLC peptide separation systems
Packaged in amber glass bottles with protective PTFE-lined fluorocarbon caps
for reagent integrity 0 Min 3 0 Min 3 0 Min 3
Has a low UV absorbance to provide the most sensitive detection across

2(a);0.000M TEA 2(b);0.010M TEA 2(c);0.020M TEA 2(d);0.030M TEA
all wavelengths
Properties of Triethylamine
Alternate names TEA, Diethylethanamine
2,4-dimethylaniline
2,4-dimethylaniline
Molecular formula CH N
2,4-dimethylaniline
6 15
Molecular weight 101.19
2,4-dimethylaniline
Density 0.726g/mL
0 Min 3 0 Min 3 0 Min 3 0 Min 3

Triethylamine (TEA)
Triethylamine, Sequencing Grade 100g X TS-25108 1 Each
Formic Acid Ampules
Well suited for HPLC and mass spectrometry applications
Formic acid is a component found in reverse-phase mobile phases to provide protons for
LC-MS analysis. The presence of a low concentration of formic acid in the mobile phase
is also know to improve the peak shapes of the resulting separation. Unlike trifluoroacetic
acid (TFA), formic acid is not an ion-pairing reagent, and it does not suppress MS ionization
of polypeptides when used as a mobile phase component.
Prescored, nitrogen-flushed, amber glass to protect formic acid from light and moisture
99% purity for consistent LC baselines and no interference introduced into LC and mass
spectrometry applications
Convenient format simplifies preparation of gradient and isocratic mobile phases containing
0.1% (v/v) formic acid in water or acetonitrile
Contents of a single vial in a final volume of 1L solvent yields a mobile phase of the most
common formic acid concentration
Formic Acid Ampules

Formic Acid 99+% 10 x 1mL ampules TS-28905 1 Each
For complex peptide separations, the key to success can be to vary selectivity. Varying mobile phase
composition on the same column can change selectivity enough to resolve peptides that would otherwise
overlap. The TFA concentration is usually specified as 0.1% for reverse-phase HPLC of peptides. For
reproducible separations from run-to-run or from lab-to-lab, it is essential to make concentrations the same.
4-102
Derivatization and Visualization Reagents for HPLC

Trifluoracetic Acid (TFA)
Routinely used ion-pairing agent in reversed-phase peptide separations
Purity: >99.5% TFA and exceptional clarity allows sensitive, non-destructive peptide
detection at low UV wavelengths
High-performance packaging: Packaged under nitrogen in amber glass with protective TFE-lined
fluorocarbon caps to ensure TFA integrity
Choice of formats for convenience: 1mL ampules can prepare 1L of 0.1% v/v TFA solution for
the mobile phase in reversed-phase chromatography in moments
Trifluoracetic Acid (TFA)

Trifluoracetic Acid, Sequencing Grade 500mL X TS-28901 1 Each
Trifluoracetic Acid, Sequencing Grade 100g X TS-28903 1 Each
Trifluoracetic Acid, Sequencing Grade 10 1mL X TS-28904 1 Pack
Trifluoracetic Acid, Sequencing Grade 1g X TS-28902 1 Each
LC Reagents
FDAA, Marfeys Reagent

Makes separation and quantitation of optical isomers of amino acids by reversed-
phase chromatography quick and easy
Optical isomers of amino acids derivatization complete in just 90 minutes

Derivatives have an absorption coefficient of ~3 x 104
Derivatives can be detected by UV at 340nm with picomole sensitivity
FDAA, Marfeys Reagent

FDAA, Marfeys Reagent 50mg TS-48895 1 Each
L-Methionine
Excess Reagent
D-Glutamic Acid
D-Phenyalanine
D-Alanine
D-Aspartic Acid
L-Glutamic Acid
L-Phenylalanine
D-Methionine
Absorbance @ 340 nm
L-Alanine
L-Aspartic Acid
10 20 30 40
p-Bromophenacylate Reagent
Gives quantitative yields with few or no side reactions
Premixing of phenacylbromide and crown ether is not necessary

Derivatization is both rapid and quantitative, with yields of >95% in 15 to 20 minutes at 80C
Excess reactants do not interfere
Large excess of alkylating reagent is not necessary
Small amounts of water or alcohol do not interfere
If isolation is desired, products are usually crystalline
p-Bromophenacylate Reagent
p-Bromophenacylate Reagent 10mL TS-48891 1 Each
TNBSA (Trinitrobenzene Sulfonic Acid)

An excellent choice for spectrophotometric detection
Couples with primary amines, sulfhydryls and hydrazides in aqueous solution at pH 8, without
undesirable side reactions
Excellent for solution or solid phase analysis
Suitable for qualitative and quantitative estimation of biomolecules; including amino acids,
eptides or proteins
Chromogenic, O = 335nm
max
Colored derivatives are monitored at 345nm and have extinction coefficients in range of 1-1.5 x 104
TNBSA

TNBSA 100mL X TS-28997 1 Each
4-104
Hydrolysis Reagents

Constant Boiling (6N) Hydrochloric Acid
Sequencing-grade reagent for total protein hydrolysis
Hydrolyzes peptides in 6 hours at 150C

Specially purified to give ninhydrin-negative blank on hydrolysis
Packaged in prescored ampules to eliminate contamination and ensure product integrity
Constant Boiling (6N) Hydrochloric Acid

Hydrochloric Acid 6N 10 1mL TS-24308 1 Pack
Amino Acid Standard H

High-purity calibration standard for protein hydrolysates
LC Reagents
Uses L-form configuration to permit standardization of microbial and other assays
Molar concentration verified by conventional amino acid analysis methods
With the exception of cystine, each amino acid is supplied at a concentration
of 2.5moles/mL in 0.1N HCl
The following amino acids are included in Amino Acid Standard H:
L-Alanine, Ammonia [(NH4)2SO4], L-Arginine, L-Aspartic Acid, L-Cystine, L-Glutamic Acid,
Glycine, L-Histidine, L-Isoleucine, L-Leucine, L-LysineHCl, L-Methionine,
L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tyrosine, L-Valine.
Amino Acid Standard H

Amino Acid Standard H 10 1mL TS-20088 1 Pack
When kept frozen, an unopened vial has an indefinite storage life. Once the seal is broken, the reagent has a
maximum storage life of six months. Store frozen between uses.
Amino Acid Detection Reagents
Ninhydrin
The reagent of choice for detection of amino acids
Used in amino acid chromatography

Offers superb color response and low blank
Indefinitely stable and requires no refrigeration
Ninhydrin
Ninhydrin 500g TS-21003 1 Each
Indefinitely stable. No refrigeration required. Keep bottle tightly sealed. Avoid exposure to direct sunlight and
ammonia.
HPLC and Spectrophotometric Grade Solvents

Ultrapure solvents are carefully packed for thorough protection
Distilled in glass, filtered through 0.2m TFE membranes and packed in solvent-rinsed,
amber glass bottles
TFE-lined screw caps seal bottles
Acetonitrile, HPLC Grade, Physical Dimethylformamide (DMF), Sequencing Dimethylsulfoxide (DMSO), Sequencing
Properties Grade, Physical Properties Grade, Physical Properties
UV Cutoff: 190nm HCON(CH3)2 C2H6OS
Optical Absorbance: <0.02 at 220nm Purity (GC): 99% Purity (GC): >99.5%
Refractive Index at 25C: 1.342 MW: 73.09 MW: 78.13
Water, HPLC Grade, Physical Properties Density: 0.944 Density: 1.101
UV Cutoff: 190nm B.P. 153C Water: 0.2%

Optical Absorbance: <0.005 at 220nm Water: 0.1% Pyridine, Physical Properties

Refractive Index at 25C: 1.332 C5H5N
Purity (GC): 99%
MW: 79.10
Density: 0.978
B.P. 115C
HPLC and Spectrophotometric Grade Solvents

Acetonitrile 1L X TS-51101 1 Each
Water 1L TS-51140 1 Each
Dimethylformamide (DMF) 50mL X TS-20673 1 Each
Dimethylsulfoxide (DMSO) 950mL X TS-20688 1 Each
Pyridine 100g X TS-25104 1 Each
4-106
High-Purity Pre-Column Derivatization Reagents

PITC (Phenylisothiocyanate)
High-purity reagent for pre-column quantitative derivatization of amino acids
by reversed-phase HPLC
Also known as Edmans Reagent

Reacts readily with amino acids in 5 to 10 minutes at room temperature
Resulting phenylthiocarbamyl derivatives can be separated and quantified in 30 minutes
using reverse-phase HPLC to produce stable products with all amino acids including proline
PITC (Phenylisothiocyanate)
PITC (Edmans Reagent) 10 1mL TS-26922 1 Pack
LC Reagents
Peptide Standards
Peptide Retention Standard

Allows accurate prediction of elution time for peptides of known amino acid
composition up to 20 residues in length
Save time in peptide purification

Simplify identification of specific peptides in a complex mixture
Increase the efficiency of predicting peptide elution profiles
Determine the relative order of peptide elution of a complex mixture
Evaluate reverse-phase supports of varying n-alkyl chain lengths and ligand densities
Retention times are predicted by totaling the values that represent the contribution in minutes of each
amino acid residue and the peptide terminal groups. Retention time is dependent upon the molecular
weight of the peptide. The effect on retention is relatively unimportant with a small peptide, but it
increases with the size of the molecule. The accuracy of predicting peptide retention time significantly
decreases beyond 20 residues. To ensure accuracy, a peptide standard is used to correct for instrument
variation, column aging, n-alkyl chain length variation and ligand density.
Peptide Retention Standard

Peptide Retention Standard, S1-S5 1 vial TS-31700 1 Each
Peptide Retention Time Calibration Mixture

Heavy peptide mixture for column assessment and prediction of peptide retention times
Assessment of chromatography and MS instrument performance

Prediction of peptide retention across multiple instrument platforms
Prediction of peptide retention time from sequence using calculated hydrophobicity factor
Optimization of scheduled MS acquisition windows for improving quantification and increased multiplexing
Internal standard to normalize for variation in retention times and peak intensities between runs
Peptide Retention Time Calibration Mixture

Peptide Retention Time Calibration
50L TS-88320 1 Each
Mixture, 0.5pmol/L
Peptide Retention Time Calibration
200L TS-88321 1 Each
Mixture, 5pmol/L
4-108
LC and LC-MS Instruments

Our liquid chromatographs and mass spectrometers advance scientific
knowledge, enable drug discovery, improve manufacturing processes and
protect people and the environment. You can expect the best results and
highest productivity, keeping your research or processes moving smoothly.
Vanquish Flex System

The Thermo Scientific Vanquish Flex system is a biocompatible UHPLC system with state-of-the-art
quaternary solvent blending, ideally suited for the biopharma industry.
The Vanquish UHPLC platform is designed to set new benchmarks on accuracy, precision, and sensitivity
in liquid chromatography. As a new part of this family, the Vanquish Flex system shares all Vanquish-
inherent values, such as a design focus on uptime, robustness and reliability.

Vanquish System
The Vanquish system takes high-end UHPLC to a new level, offering more resolution while meeting
the throughput demands of modern laboratories. The system is driven by the gold standard
Thermo Scientific Dionex Chromeleon Chromatography Data System (CDS) software and
delivers better separations, more results and easier interaction. The Vanquish system delivers
these values simultaneously, without compromise.
When combined with the Thermo Scientific Charger module, the Vanquish system allows for added
sample capacity, performing the highest throughput UHPLC analysis. This setup combines enough
sample capacity to run over the weekend, while Chromeleon CDS ensures convenient sample tracking
and results processing of even the largest sample sets.
Visit www.thermoscientific.com/vanquish
Basic Automated and Standard (SD) Systems

The Thermo Scientific Dionex UltiMate 3000 Basic Automated System is optimized
for reliability and ease-of-use with routine LC applications, and also offers full UHPLC
compatibility. The ACC-3000 Autosampler Column Compartment is at the heart of the
system. Its unique instrument design combines a rugged sample injection principle
with a powerful column oven.
UltiMate 3000 Standard (SD) Systems are designed to meet current and future
challenges. System components provide low extra-column and gradient delay volume
for high separation efficiency and low gradient response times, as well as superior
mixing performance. They offer full support of all HPLC applications and provide
UHPLC compatibility allowing you to move to UHPLC whenever you are ready.
Visit www.thermoscientific.com
Rapid Separation (RS) and BioRS Systems

UltiMate 3000 Rapid Separation (RS) Systems provides unrivaled performance and flexibility in UHPLC.
Precision-engineered instrumentation, advanced data processing, and highly optimized chemistries meet
all chromatographic performance challenges. With an outstanding product portfolio, UltiMate 3000 RS
Systems offers industry-leading versatility covering the maximum range of UHPLC applications, while
keeping fully compatible to conventional LC.
UltiMate 3000 Biocompatible Rapid Separation (BioRS) Systems are powered by UltiMate 3000 Rapid
Separation technology to support the high pressures required for the separation of bioanalytes on high
resolution bio UHPLC columns. This state-of-the-art technology combined with a biocompatible,
low-dispersion flow path, provides the highest peak capacity and sensitivity for complex samples,
whether proteins, peptides, or biotherapeutics. Bio UHPLC enables faster gradients and shorter
run times compared to conventional Bio LC analysis. This results in increased sample throughput
in typical modes of biochromatography.
x2 Dual LC Systems
UltiMate 3000 x2 Dual LC Systems are the ideal choice to maximize sample throughput of conventional
HPLC and UHPLC separations by combining the flexibility of ternary solvent selection with the productivity
and performance of x2 Dual technology.
Operate conventional or high speed applications, increase sample throughput, achieve higher
chromatographic resolution, or automate tedious sample preparation steps such as analyte enrichment
or matrix elimination.
4-110
Thermo Scientific LC-MS Systems

Ion Traps and Orbitrap Series
Thermo Scientific Orbitrap LC-MS systems are the recognized standards
for high-resolution, accurate-mass measurements.
Orbitrap LC-MS technology routinely delivers the superior mass resolution and mass accuracy necessary
to reduce analysis times and increase confidence in results. This makes it the platform of choice for the
most confident protein and metabolite identification, characterization and quantitation. Combined with
superior dynamic range and unsurpassed sensitivity, Orbitrap systems are the only mass spectrometers
capable of providing all four benefits at the same time, without compromise.
With market and technology leadership for over 25 years, we offer ion trap LC-MS systems for every
application and budget. Ion trap LC-MS systems offer unique capabilities such as MSn and data-
dependent analysis along with excellent full-scan sensitivity to provide routine detection and
rapid identification of low-level analytes. Ion trap systems support a variety of applications from
compound identification and routine HPLC detection through the most demanding analyses of
low-level components in complex biological matrices.

Visit www.thermoscientific.com/orbitrap
TSQ Series Triple Quadrupole LC-MS

Robust triple-stage quadrupole LC-MS systems enable ultra-sensitive
quantitation of target compounds in complex matrices.
TSQ Series triple-stage quadrupole LC-MS systems offer a wide range of capabilities. From the
value-conscious Thermo Scientific TSQ Endura LC-MS to the precise Thermo Scientific TSQ Quantiva
LC-MS, our mass spectrometers couple perfectly with our HPLC, UHPLC, and nano-LCs to easily
accommodate complex matrices encountered in a variety of applications.
Visit www.thermoscientific.com/tsq
MSQ Plus Single Quadrupole LC-MS

Our single quadrupole LC-MS system offers superior ease-of-use and modest
price and space requirements.
The system can be used with both HPLC and IC systems in a wide range of applications
and methodologies.
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reasonable control, Seller reserves the right to terminate the order or to reschedule the shipment within a modify, enhance or otherwise change or supplement the software products provided hereunder without Sellers
reasonable period of time, and Buyer will not be entitled to refuse delivery or otherwise be relieved of any prior written consent. Seller will be entitled to terminate this license if Buyer fails to comply with any term or
obligations as the result of such delay. Products as to which delivery is delayed due to any cause within Buyers condition herein. Buyer agrees, upon termination of this license, immediately to return to Seller all software
control may be placed in storage by Seller at Buyers risk and expense and for Buyers account. Orders in process products and related documentation provided hereunder and all copies and portions thereof.
may be canceled only with Sellers written consent and upon payment of Sellers cancellation charges. Orders in Certain of the software products provided by Seller may be owned by one or more third parties and licensed to
process may not be changed except with Sellers written consent and upon agreement by the parties as to an Seller. Accordingly, Seller and Buyer agree that such third parties retain ownership of and title to such software
appropriate adjustment in the purchase price therefor. Credit will not be allowed for Products returned without products. The warranty and indemnification provisions set forth herein shall not apply to software products
the prior written consent of Seller. owned by third parties and provided hereunder.
6. TITLE AND RISK OF LOSS. Notwithstanding the trade terms indicated above and subject to Sellers right to 10. LIMITATION OF LIABILITY. NOTWITHSTANDING ANYTHING TO THE CONTRARY CONTAINED HEREIN,
stop delivery of Products in transit, title to and risk of loss of the Products will pass to Buyer upon delivery of THE LIABILITY OF SELLER UNDER THESE TERMS AND CONDITIONS (WHETHER BY REASON OF BREACH OF
possession of the Products by Seller to the carrier; provided, however, that title to any software incorporated CONTRACT, TORT, INDEMNIFICATION, OR OTHERWISE, BUT EXCLUDING LIABILITY OF SELLER FOR BREACH
within or forming a part of the Products shall at all times remain with Seller or the licensor(s) thereof, as the case OF WARRANTY (THE SOLE REMEDY FOR WHICH SHALL BE AS PROVIDED UNDER SECTION 7 ABOVE)) SHALL
may be. NOT EXCEED AN AMOUNT EQUAL TO THE LESSER OF (A) THE TOTAL PURCHASE PRICE THERETOFORE PAID BY
7. WARRANTY. Seller warrants that the Products will operate or perform substantially in conformance with BUYER TO SELLER WITH RESPECT TO THE PRODUCT(S) GIVING RISE TO SUCH LIABILITY OR (B) ONE MILLION
Sellers published specifications and be free from defects in material and workmanship, when subjected to DOLLARS ($1,000,000). NOTWITHSTANDING ANYTHING TO THE CONTRARY CONTAINED HEREIN, IN NO
normal, proper and intended usage by properly trained personnel, for the period of time set forth in the product EVENT SHALL SELLER BE LIABLE FOR ANY INDIRECT, SPECIAL, CONSEQUENTIAL OR INCIDENTAL DAMAGES
documentation, published specifications or package inserts. If a period of time is not specified in Sellers product (INCLUDING WITHOUT LIMITATION DAMAGES FOR LOSS OF USE OF FACILITIES OR EQUIPMENT, LOSS OF
documentation, published specifications or package inserts, the warranty period shall be one (1) year from the REVENUE, LOSS OF DATA, LOSS OF PROFITS OR LOSS OF GOODWILL), REGARDLESS OF WHETHER SELLER (a)
date of shipment to Buyer for equipment and ninety (90) days for all other products (the Warranty Period). HAS BEEN INFORMED OF THE POSSIBILITY OF SUCH DAMAGES OR (b) IS NEGLIGENT.
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model and serial number (if applicable) and details of the warranty claim; and (b) after Sellers review, Seller will Export Administration Regulations of the U.S. Department of Commerce (the EAR), which may restrict or
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decontamination procedures and other product-specific handling instructions, then, if applicable, Buyer may shall comply with the EAR and all other applicable laws, regulations, laws, treaties, and agreements relating to
return the defective Products to Seller with all costs prepaid by Buyer. Replacement parts may be new or the export, re-export, and import of any Item. Buyer shall not, without first obtaining the required license to do
refurbished, at the election of Seller. All replaced parts shall become the property of Seller. Shipment to Buyer so from the appropriate U.S. government agency; (i) export or re-export any Item, or (ii) export, re-export,
of repaired or replacement Products shall be made in accordance with the Delivery provisions of the Sellers distribute or supply any Item to any restricted or embargoed country or to a person or entity whose privilege to
Terms and Conditions of Sale. Consumables are expressly excluded from this warranty. participate in exports has been denied or restricted by the U.S. government. Buyer shall, if requested by Seller,
Notwithstanding the foregoing, Products supplied by Seller that are obtained by Seller from an original provide information on the end user and end use of any Item exported by the Buyer or to be exported by the
manufacturer or third party supplier are not warranted by Seller, but Seller agrees to assign to Buyer any Buyer. Buyer shall cooperate fully with Seller in any official or unofficial audit or inspection related to applicable
warranty rights in such Product that Seller may have from the original manufacturer or third party supplier, to the export or import control laws or regulations, and shall indemnify and hold Seller harmless from, or in connection
extent such assignment is allowed by such original manufacturer or third party supplier. with, any violation of this Section by Buyer or its employees, consultants, or agents.
In no event shall Seller have any obligation to make repairs, replacements or corrections required, in whole or in 12. MISCELLANEOUS. (a) Buyer may not delegate any duties nor assign any rights or claims hereunder without
part, as the result of (i) normal wear and tear, (ii) accident, disaster or event of force majeure, (iii) misuse, fault or Sellers prior written consent, and any such attempted delegation or assignment shall be void. (b) The rights and
negligence of or by Buyer, (iv) use of the Products in a manner for which they were not designed, (v) causes obligations of the parties hereunder shall be governed by and construed in accordance with the laws of the State
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covered by the warranty hereunder, Buyer shall pay or reimburse Seller for all costs of investigating and venue to which it may be entitled by domicile or otherwise. (c) In the event of any legal proceeding between the
responding to such request at Sellers then prevailing time and materials rates. If Seller provides repair services Seller and Buyer relating to this Agreement, neither party may claim the right to a trial by jury, and both parties
or replacement parts that are not covered by this warranty, Buyer shall pay Seller therefor at Sellers then waive any right they may have under applicable law or otherwise to a right to a trial by jury. Any action arising
prevailing time and materials rates. ANY INSTALLATION, MAINTENANCE, REPAIR, SERVICE, RELOCATION OR under this Agreement must be brought within one (1) year from the date that the cause of action arose. (d) The
ALTERATION TO OR OF, OR OTHER TAMPERING WITH, THE PRODUCTS PERFORMED BY ANY PERSON OR application to this Agreement of the U.N. Convention on Contracts for the International Sale of Goods is hereby
ENTITY OTHER THAN SELLER WITHOUT SELLERS PRIOR WRITTEN APPROVAL, OR ANY USE OF expressly excluded. (e) In the event that any one or more provisions contained herein shall be held by a court of
REPLACEMENT PARTS NOT SUPPLIED BY SELLER, SHALL IMMEDIATELY VOID AND CANCEL ALL competent jurisdiction to be invalid, illegal or unenforceable in any respect, the validity, legality and
WARRANTIES WITH RESPECT TO THE AFFECTED PRODUCTS. enforceability of the remaining provisions contained herein shall remain in full force and effect, unless the
THE OBLIGATIONS CREATED BY THIS WARRANTY STATEMENT TO REPAIR OR REPLACE A DEFECTIVE revision materially changes the bargain. (f) Sellers failure to enforce, or Sellers waiver of a breach of, any
PRODUCT SHALL BE THE SOLE REMEDY OF BUYER IN THE EVENT OF A DEFECTIVE PRODUCT. EXCEPT AS provision contained herein shall not constitute a waiver of any other breach or of such provision. (g) Unless
EXPRESSLY PROVIDED IN THIS WARRANTY STATEMENT, SELLER DISCLAIMS ALL OTHER WARRANTIES, otherwise expressly stated on the Product or in the documentation accompanying the Product, the Product is
WHETHER EXPRESS OR IMPLIED, ORAL OR WRITTEN, WITH RESPECT TO THE PRODUCTS, INCLUDING intended for research only and is not to be used for any other purpose, including without limitation, unauthorized
WITHOUT LIMITATION ALL IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR ANY PARTICULAR commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption by or
PURPOSE. SELLER DOES NOT WARRANT THAT THE PRODUCTS ARE ERROR-FREE OR WILL ACCOMPLISH ANY application to humans or animals. (h) Buyer agrees that all pricing, discounts and technical information that
PARTICULAR RESULT. Seller provides to Buyer are the confidential and proprietary information of Seller. Buyer agrees to (1) keep such
information confidential and not disclose such information to any third party, and (2) use such information solely
8. INDEMNIFICATION.
for Buyers internal purposes and in connection with the Products supplied hereunder. Nothing herein shall
8.1 By Seller. Seller agrees to indemnify, defend and save Buyer, its officer, directors, and employees from and restrict the use of information available to the general public. (i) Any notice or communication required or
against any and all damages, liabilities, actions, causes of action, suits, claims, demands, losses, costs and permitted hereunder shall be in writing and shall be deemed received when personally delivered or three (3)
expenses (including without limitation reasonable attorneys fees) (Indemnified Items) for (i) injury to or death business days after being sent by certified mail, postage prepaid, to a party at the address specified herein or at
of persons or damage to property to the extent caused by the negligence or willful misconduct of Seller, its such other address as either party may from time to time designate to the other.
employees, agents or representatives or contractors in connection with the performance of services at Buyers
6-032
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Thermo Scientific

Accucore Hypersil GOLD

LC Columns and Accessories >>

and Analytical HPLC

Hydrophobicity Key Attribute Achieved through

Syncronis Columns 1.7, 3 and 5m high

Page 4-062 surface area silica

Ultimate Core Performance

Solid Core Particles

Tight Control of Particle Diameter

The Accucore web page contains the latest news, applications

Powerful separations are our core performance

Thermo Scientific Accucore Vanquish UHPLC columns provide a robust

Mobile Phase A: Water

100 2.1mm = 380L/min

3. Hexazinone 12. Metobromuron

Comparison of separation of 18 pesticides using Accucore Vanquish 1.5m column

LC Columns and Accessories >>

5.0 Naproxen Fenoprofen Ibuprofen

RSD for retention time less than 0.3%

Optimized for MS detection 0 2 to 9 14

100 17626-102130 17626-103030 17626-104630

LC Columns and Accessories >>

analytes via a predominantly hydrophobic interaction mechanism. Carbon Load (%)

Lower hydrophobic retention 0 2 to 9 14

Recommended Pore Size

phase provides optimum retention. Particle Size 2.6m USP L7

The low levels of secondary interactions demonstrated in the phase characterization

100 Accucore C8 2.6m, 50 x 2.1mm

Flow Rate: 60 Flow:

%RSD Area 3.01

Data from six injections.

100 17226-102130 17226-103030 17226-104630

LC Columns and Accessories >>

mn: 14050 x 2.1 mm

1500 L/min Injection Volume: 1L

Accucore Polar Premium pH Range

Particle Size 2.6m USP L60

Rugged amide-embedded C18 phase 0 1.5 to 10 14

mobile phase Pore Size

300 Fused Core C18

HPLC Column 50 28026-052130 28026-053030 28026-054630

LC Columns and Accessories >>

The C6 chain in Accucore Phenyl-Hexyl phase exhibits classical RP retention 0 80 300

Particle Size 2.6m

Unique reversed-phase shape selectivity 0 2 to 8 14

2.6 Defender Guard (4/pk) 10 27926-01210575 - 2

UNIGUARD Drop-in 10 852-00 852-00 850-00

LC Columns and Accessories >>

Ideal for separation of hydrophobic, long alkyl chain compounds 0 2 to 8 14

Particle Size 2.6m USP L62

250 Accucore C30 2.6m, 100 x 3.0mm

-50 200 2+2

Particle Size (m) Format Length (mm) 2.1mm ID 3.0mm ID 4.6mm ID

2.6 Defender Guard (4/pk) 10 27826-012105 0 - -

LC Columns and Accessories >>

2 Accucore HILIC 2.6m, 50mm x 2.1mm

600 Accucore Urea-HILIC 2.6m, 100 x 2.1mm

Flow Rate: 300L/min

Syncronis Columns 1.7, 3 and 5m high

Solutions for all