Nothing Special   »   [go: up one dir, main page]
Scribd Logo
Upload

Welcome to Scribd!

  • 213 views

    De Anemia Factsheet Ferritin

    Uploaded by

    yantu
    elecsys feritin

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    De Anemia Factsheet Ferritin

    Uploaded by

    yantu
    213 views2 pages
    elecsys feritin

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    elecsys feritin

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    213 views2 pages

    De Anemia Factsheet Ferritin

    Uploaded by

    yantu
    elecsys feritin

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Elecsys Ferritin

    Electrochemiluminescence immunoassay (ECLIA)


    for the in vitro quantitative determination of ferritin
    in human serum or plasma
    Indication
    Ferritin is the iron storage protein. The ferritin detectable in human serum is in equilibrium with the bodys depot iron and
    hence acts as an indicator for the level of iron stores. Determination of Ferritin can be used as an aid in iron metabolism
    diagnosis, monitoring iron therapy, ascertaining the iron reserves in groups at risk and in the differential diagnosis of anemias.

    Test principle: Sandwich principle

    Ferritin in Streptavidin
    the sample microparticle

    Ru 9 min 9 min Ru Measurement

    Biotinylated Ruthenylated
    monoclonal monoclonal
    Ru
    Ferritin-specific Ferritin-specific
    antibody antibody

    1st incubation (9 minutes) 2nd incubation (9 minutes)


    10 L of sample, a biotinylated monoclonal ferritinspecific After addition of streptavidin-coated microparticles, the
    antibody, and a monoclonal ferritinspecific antibody labeled complex becomes bound to the solid phase via interaction
    with a ruthenium complex form a sandwich complex. of biotin and streptavidin.

    Measurement
    The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the
    surface of the electrode. Unbound substances are then removed. Application of a voltage to the electrode then induces
    chemiluminescent emission which is measured by a photomultiplier.

    Elecsys technology
    ECL (ElectroChemiLuminescence) is Roches technology for immunoassay detection. Based on this technology and combined
    with well-designed, specific and sensitive immunoassays, Elecsys delivers reliable results. The development of ECL immuno
    assays is based on the use of a ruthenium-complex and tripropylamine (TPA). The chemiluminescence reaction for the
    detection of the reaction complex is initiated by applying a voltage to the sample solution resulting in a precisely controlled
    reaction. ECL technology can accommodate many immunoassay principles while providing superior performance.
    Elecsys Ferritin test characteristics
    Testing time 18 minutes
    Test principle Sandwich assay
    Calibration 2 point
    Traceability Standardized against the Enzymun-Test Ferritin method,
    which has been standardized against the 1st International
    Standard (IS) NIBSC (National Institute for Biological
    Standards and Control) Reagent for Ferritin (human liver)
    80/602. Recent recovery studies show very good agreement
    with 2nd IS 80/578 and 3rd IS 94/572.
    Sample material Serum collected using standard sampling tubes. Li-,
    Naheparin, K3EDTA and sodium citrate plasma. When
    sodium citrate is used, the results must be corrected by
    + 10%.
    Sample volume 10 L
    Detection limit 0.50 g/L (ng/mL)
    Measuring range 0.5002000 g/L (ng/mL)
    Intermediate imprecision cobas e 411 analyzer: 2.17.7%
    cobas e 601/e 602 modules: 2.58.1%
    Lowest conc. measured: 11.9 g/L (ng/mL)
    Expected values Men, 2060 years: 30400 g/L (ng/mL)
    Women, 1760 years: 13150 g/L (ng/mL)

    Order information
    Elecsys Ferritin 100 tests per rackpack 04491785 190
    Elecsys Ferritin 200 tests per rackpack 04491785 190
    Ferritin CalSet 12 mL each of CalSet Calibrator 1 and 2 03737586 190
    PreciControl Varia 23 mL each of PreciControl Varia 05618860 190
    1 and 2

    COBAS, COBAS E, ELECSYS and LIFE NEEDS ANSWERS are trademarks of Roche.

    2014 Roche

    Roche Diagnostics International Ltd


    CH-6343 Rotkreuz
    Switzerland
    www.cobas.com

    You might also like