Hematology Laboratory PDF
Hematology Laboratory PDF
Hematology Laboratory PDF
HEMATOLOGY
LABORATORY
HEMATOLOGY
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LABORATORY
HEMATOCRIT ESR
Parallel to hemoglobin Erythrocyte Sedimentation Rate
Volume occupy by erythrocytes in the given volume of blood Rate of setting of RBC from the plasma after an addition of
Useful in determining erythrocyte indices and for calculation of a anticoagulant
blood volume 9 Very useful to establish anemia; & other disorder in blood Importance to measure rate of fall
9 MCH & MCV Hemoglobin, Hematocrit & RBC Associated with the net-surface charge of RBC with normal
Useful for total erythrocyte mass determination surface of charge negative
Useful in establishing anemia
IMPORTANCE OF ESR
METHODS FOR HEMATOCRIT Use as a good index for determination of a hidden but active
1) Macro method Use large volume of blood disease such as tuberculosis and carcinoma
a) Winthrobe method Measures the suspension stability of the RBC
Uses Winthrobe tube Measures the abnormal concentration of fibrinogen and protein
Flat bottom tube graduated at both side globulin
Fill the tube with blood sample using capillary pipet
Uses oxalated blood sample ESR METHODS
Alternative anticoagulant: EDTA 1. Winthrobe Method
Centrifuge the tube with the speed of 3,500rpm for Uses double oxalate; alternative: EDTA
30minutes Fill in the capillary pipet, stand for an hour
Poikilocytosis PA
!
TH
Sickle cell anemia OL
OG
Severe Iron Deficiency Anemia (IDA)
!
I CA
Thalassemia L
!
Jaundice
ESR$Scale$100$!!!!
Note:
The first drop of blood is usually discarded because it is
contaminated with dead epidermal cells and tissue juices.
TRANSCRIBED GEN CAMATO
HEMATOLOGY
LABORATORY
HEMATOLOGY
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LABORATORY
HEMOCYTOMETRY
Numerical evaluation of the formed elements of blood
Estimation of the number of blood cells in a given
volume of blood
TRANSCRIBED GEN CAMATO
HEMATOLOGY
LABORATORY
HEMATOLOGY
1
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LABORATORY
! Gowers
Prevents rouleaux formation and precipitation of protein
! Toissons
Initiates mold formation so it should be filtered
With high specific gravity
With stain so used by beginners since RBC are easily
identified form WBCs whose nuclei stain are blue. A wise man will hear, and will increase learning; and a man of understanding shall
attain unto wise counsels:
- Proverbs 1:5