This document describes the calibration of an HPLC instrument. [1] Procedures are provided to calibrate the flow rate and ensure it is within 2% of the set flow rate. [2] Linearity of the detector is evaluated by injecting 5 standard solutions with concentrations ranging from 10-40 ppm and measuring the response. The correlation coefficient must be 0.98-1.000. [3] Precision is determined by 6 replicate injections of a 20 ppm standard and calculating the %RSD which must meet acceptance criteria.
This document describes the calibration of an HPLC instrument. [1] Procedures are provided to calibrate the flow rate and ensure it is within 2% of the set flow rate. [2] Linearity of the detector is evaluated by injecting 5 standard solutions with concentrations ranging from 10-40 ppm and measuring the response. The correlation coefficient must be 0.98-1.000. [3] Precision is determined by 6 replicate injections of a 20 ppm standard and calculating the %RSD which must meet acceptance criteria.
This document describes the calibration of an HPLC instrument. [1] Procedures are provided to calibrate the flow rate and ensure it is within 2% of the set flow rate. [2] Linearity of the detector is evaluated by injecting 5 standard solutions with concentrations ranging from 10-40 ppm and measuring the response. The correlation coefficient must be 0.98-1.000. [3] Precision is determined by 6 replicate injections of a 20 ppm standard and calculating the %RSD which must meet acceptance criteria.
This document describes the calibration of an HPLC instrument. [1] Procedures are provided to calibrate the flow rate and ensure it is within 2% of the set flow rate. [2] Linearity of the detector is evaluated by injecting 5 standard solutions with concentrations ranging from 10-40 ppm and measuring the response. The correlation coefficient must be 0.98-1.000. [3] Precision is determined by 6 replicate injections of a 20 ppm standard and calculating the %RSD which must meet acceptance criteria.
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The document describes the procedure for calibrating an HPLC instrument, including calibrating the flow rate, linearity, precision, and wavelength accuracy.
The document is describing a standard operating procedure for calibrating an HPLC instrument.
The document is calibrating the flow rate, linearity, precision, and wavelength accuracy of the HPLC instrument.
Document Title : HPLC CALIBRATION
Reference SOP : PROCEDURE FOR OPERATION&CALIBRATION OF HPLC
Reference SOP No. : PB/SOP/QCD/001/01 Format No. : F/PB/QCD/001/01 Annexure No. : III Page No. : 1 of 1
HPLC ID No: ------------------------------- Column ID No : ------------------------- Make : ------------------------------ Model : -------------------------- Date of calibration: ------------------------ Calibration due Date: --------------------------- Frequency : Half Yearly 1. FLOW RATE: Weight per ml of water Wt of Empty Pycnometer g Wt / ml
= g/ml Wt of Filled Pycnometer g Wt of Water g 25.0
Set flow Wt. of empty Cylender (W1) Wt. of Cylender + Water after 10 minutes (W2) Flow rate (ml/min)= [(W2-W1)/10 x wt/ml] Average flow Rate (ml/min) Limit + 2.0% Minimum (ml/min) Maximu m (ml/min) 1.0 ml/min g g 0.98 1.02 g g g g 2.0 ml/min g g 1.96 2.04 Document Title : HPLC CALIBRATION Reference SOP : PROCEDURE FOR OPERATION&CALIBRATION OF HPLC Reference SOP No. : PB/SOP/QCD/001/01 Format No. : F/PB/QCD/001/01 Annexure No. : III Page No. : 2 of 1
Sample Solution A : Weigh accurately about ____(100 mg) of Caffeine Anhydrous Pure in 100 ml of Volumetric Flask, dissolve and make up to mark with Mobile Phase.
Sample Solution B: dilute 1 ml of this solution A to 100 ml with Mobile Phase (10 ppm).
Sample Solution C: dilute 2 ml of this solution B to 100 ml with Mobile Phase (20 ppm).
Sample Solution D: dilute 3 ml of this solution C to 100 ml with Mobile Phase (30 ppm).
Sample Solution E: D dilute 4 ml of this solution D to 100 ml with Mobile Phase (40 ppm).
Document Title : HPLC CALIBRATION Reference SOP : PROCEDURE FOR OPERATION&CALIBRATION OF HPLC Reference SOP No. : PB/SOP/QCD/001/01 Format No. : F/PB/QCD/001/01 Annexure No. : III Page No. : 3 of 1
S.No Concentrati on Area Mean Area Correlation Coefficient (r) Acceptance Criteria 01 10 ppm
0.98 to 1.000
02 20 ppm
03 30ppm
04 40ppm
05 50ppm
Calibrated by: ------------------- Checked by: -------------------- Date : ----------------- Date : -------------------
Document Title : HPLC CALIBRATION Reference SOP : PROCEDURE FOR OPERATION&CALIBRATION OF HPLC Reference SOP No. : PB/SOP/QCD/001/01 Format No. : F/PB/QCD/001/01 Annexure No. : III Page No. : 4 of 1
3. Precision (Detector) Sample Solution (20 ppm) : Dilute 2.0 ml of Solution A to 100ml with Mobile Phase (20 ppm). 6Replicates ,Inj
Inj. No Area Retention Time 01 02 03 04 05 06 Average STDEV % of RSD
Calibrated by: ------------------- Checked by: -------------------- Date : ----------------- Date : -------------------
Document Title : HPLC CALIBRATION Reference SOP : PROCEDURE FOR OPERATION&CALIBRATION OF HPLC Reference SOP No. : PB/SOP/QCD/001/01 Format No. : F/PB/QCD/001/01 Annexure No. : III Page No. : 5 of 1
4. Wavelenght Accuracy: Sample Solution A : Weigh accurately about ____(100 mg) of Caffeine Anhydrous Pure in 100 ml of Volumetric Flask, dissolve and make up to mark with Mobile Phase. Sample Solution B: Further dilute 2 ml of this solution to 100 ml with Mobile Phase (20 g/ml). S.No Wavelength (nm) Area Maxima at Acceptance Criteria 1 270
273nm 2 271 3 272 4 273 5 274 6 275 7 276
Instrument Calibration meets the requirement /doesn`t meets the requirements Calibrated by: ------------------- Checked by: -------------------- Date : ----------------- Date : -------------------
Document Title : HPLC CALIBRATION Reference SOP : PROCEDURE FOR OPERATION&CALIBRATION OF HPLC Reference SOP No. : PB/SOP/QCD/001/01 Format No. : F/PB/QCD/001/01 Annexure No. : III Page No. : 6 of 1
(Brill's Japanese Studies Library 40) Sorensen, Joseph T - Optical Allusions - Screens, Paintings, and Poetry in Classical Japan (Ca. 800-1200) - Brill (2012)