Comprehensive Pharmacy Review - Notes
Comprehensive Pharmacy Review - Notes
Comprehensive Pharmacy Review - Notes
Product development
New chemical entities
Preformulation: Physical and chemical characterization of the drug and dosage form during preclinical phase. Includes general properties (particle size / shape, polymorphism, crystalline structure, density, surface area, hygroscopicity), solubility (dissolution, pH-solubility profile, various solvents), chemical properties (surface energy, pH stability profile, pKa, temperature stability, excipient interactions), stability analytical methods. Formulation development: continuing process. Injections: final formulation is developed in preclinical phase, stability in solution is critical, few excipients allowed, no bioavailability for IV. Topicals / local: final formulation developed in phase I, study release in in vitro diffusion cell models, local irritation and systemic absorption are the issues. Topicals / systemic: drug delivery through skin / mucosa / rectum, final formulation in phase III.
Oral drugs: final formulation in phase II. Final product considerations: size, shape, color, taste, skin feel, viscosity, physical appearance, production equipment / site.
Preapproval inspections
Manufacturing facility is inspected prior to NDA / ANDA approval or after a major reported change to NDA / ANDA. Includes: general cGMP inspection, reviews documentation, verifies traceability of information to documentation, consults the chemistry / manfucaturing / control (CMC) section of NDA / ANDA, make a final recommendation.
GMPs
Minimum requirements for manufacturing, processing, packing, or holding drugs. Include criteria for personnel, facilities, processes to ensure final product has the correct identity, strength, quality, purity. Quality Control (QC): department responsible for establishing process and product specifications. The QC dept test the product and verifies specs are met. This includes acceptance / rejection of incoming raw materials, packaging components, water, drug products, environmental conditions. Quality Assurance (QA): a department that determines that the systems and facilities are adequate and that written procedures are followed.
Children doses
First choice: body weight or mass and mg/kg dosing. Frieds rule for infants: (age in month / 150) x adult dose Clarks rule: (weight in lb / 150) x adult dose Childs dosage based on body surface area: (BSA in m2 / 1.73) x adult dose
Electrolyte solutions
Divalent: calcium, ferrous, magnesium, sulfate. Trivalent: aluminum, ferric, citrate. All others are monovalent.
Milliequivalents (mEq)
Definition: amount in mg equivalent to a solute equal to 0.001 of its gram equivalent weight. Unit used to express concentration of electrolytes
Milliosmoles (mOsmol)
Osmotic pressure is directly proportional to the total number of particles in solution. Unit for measuring osmotic concentration: mOsmol.
For non-electrolytes: 1 millimole = 1 mOsmol (1 molecule = 1 particle) For electrolytes: number of particles depends on degree of dissociation. Example: completely dissociated KCl 1 millimole = 2 mOsmol (2 particles, K and Cl for each molecule). Example: completely dissociated CaCl2 1 millimole = 3 mOsmol solute concentration interaction between dissolved particles actual osmolar concentration compared to ideal osmolar concentration.
Isotonic solutions
Isosmotic: solution with the same osmotic pressure. Isotonic: solution with the same osmotic pressure as body fluids. Hypotonic: solution with osmotic pressure than body fluid (opposite is hypertonic) Preparation of isotonic solutions Colligative properties (e.g. freezing point depression) are representative of the number of particles in solution. Dissolve 1 g MWt of non-electrolyte in 1 L of water depression of freezing point by -1.86 C. For electrolytes: freezing point depression = -1.86 x number of species produces upon dissociation. Freezing point depression of body fluids = -0.52 C. Take dissociation of weak electrolytes into account. In weak solutions, every 2 ions produce 1.8 ions, every 3 ions produce 2.6 ions (about 10% loss). NaCl equivalents Definition: the amount of NaCl that is equivalent to the amount of particular drug in question. Isotonic fluid: 0.9% NaCl. Example: NaCl equivalent for KCl to 0.78 1 gram KCl = 0.78 g of NaCl. Calculating amount of NaCl required to adjust isotonicity: calculate the total amount of NaCl required (volume x 0.9%) calculate the NaCl equivalent of all substances in the solution calculate and add the difference as NaCl or another material (as NaCl equivalent).
Statistics
Frequency distribution: classify individual observations into categories corresponding to fixed numeric intervals (interval frequencies) plot number of observations in each category versus category descriptor. Normal distribution: bell-shaped (Gaussian) curve. Estimates of population mean: the population mean is the best estimate of the true value. Sample mean: arithmetic average. Accuracy: degree to which measured value agrees with true value. Error (bias): difference between measured value and true value. Median: midmost value of a data distribution (average of two midmost values if even number of observations). Normal distribution median = mean. Median is less affected by outliers or skewed distribution. Mode: most frequently occurring value in a distribution, it is useful for non-normal distributions especially bimodal distributions. Estimates of variability: infinite # of observations population variance. Finite # of observations sample variance. Range: useful to describe variability only in very small number of observations. Standard deviation: square root of variance. Precision (reproducibility): degree to which replicate measurements made exactly the same way agree with each other (expressed as relative standard deviation). Standard deviation of the mean (standard error): estimate of variability or error in the mean obtained from N observations. SE = SD/(sq. root of N). Used to establish confidence intervals.
When the negative pole of a dipole approach the positive pole of another molecular attraction called dipole-dipole interaction. If similar poles approach molecular repulsion (intermolecular repulsive forces)
States of matter
Gases Molecules move in straight path at high speed until they randomly collide with another molecule, creating pressure. Intermolecular forces ~ zero. Ideal gas law: Pressure (P) x Volume (V) = number of moles (n) X Molar Gas Constant (R) X Temperature (T) Gases in pharmacy: anesthetics (nitrous oxide, halothane), compressed oxygen, liquefiable aerosol propellants (nitrogen, CO2, hydrocarbons, halohydrocarbons), ethylene oxide for sterilization of heat labile objects. Volatile liquids (ether, halothane, methoxyfurane) are used as anesthetics. Amyl nitrite (volatile liquid) is inhaled as a vasodilator in acute angina. Sublimation: a solid is heated directly to the gaseous or vapor state (or vice versa, also called deposition) without passing through the liquid state. Examples: camphor, iodine. Liquids Van der Waals intermolecular forces are sufficient to impose some ordering. Hydrogen bonding cohesion in liquids. Surface and interfacial tension Molecules at the surface of the liquid experience a net inward pull from the interior and they tend to contract. This makes liquids assume a spherical shape as it is the volume with minimum surface and least free energy. Surface free energy / surface tension: the work required to the surface area A of the liquid by 1 unit area. Example: SFE for water = 72 mN/m. Interfacial tension: at the surface of two immiscible liquids. Viscosity Viscosity = shear stress / shear rate Non-Newtonian viscosity: exhibit shear dependent or time dependent (apparent) viscosity. Shear dependent viscosity: Shear thickening (dilatancy) as in suspensions of small deflocculated particles with high solid content. Shear thinning (pseudoplastic): as in polymer solutions. Plastic (Bingham body): as in flocculated particles in concentrated suspensions that have yield value. Time dependent viscosity: yield value of plastic systems may be time dependent. Thixotropic systems are shear thinning but they do not recover viscosity after shear is removed, i.e., structural recovery is slow
compared to structural breakdown. It occurs in heterogenous systems with three dimensional structural network (gel-sol transformation). Negative (anti)thixotropy: viscosity with shear up to an equilibrium (sol-gel transformation). Solids High intermolecular forces. Crystalline solids: fixed molecular order, distinct melting point, anisotropic (properties are nto the same in all directions). Amorphous solids: randomly arranged molecules, nondistinct melting point, isotropic (properties are the same in all direction). Polymorphs: substance has more than one crystalline form. Different molecular arrangments / crystalline lattice structure, melting point, solubility, dissolution rate, density, stability. Polymorphs are common in steroids, theobroma oil, cocoa butter. Latent heat of fusion: heat absorbed when 1 g of solid melts.
Colligative properties:
Depend on the total number of ionic and nonionic solute molecules in solution. They are dependent on ionization but independent of other chemical properties of the solute. Vapor pressure depression: (Raoults law): partial vapor pressure is equal to the product of the mole fraction of the component in solution and the vapor pressure of the pure component. Boiling point elevation and freezing / melting point depression Osmotic pressure: Osmosis is the process by which solvent molecules pass through semipermeable membrane from dilute solution to concentrated solution. That is because solvent molecules have lower chemical potential in concentrated solution. Osmotic pressure is the pressure that must be applied to solution to prevent the flow of pure solvent. It is defined by the vant Hoff equation.
Buffer: a mixture of salt with acid or base that resists changes in pH when small quantities of acid or salt are added. Buffer is a combination of weak acid and its conjugate base (salt) (more common), or a weak base and its conjugate acid (salt). Buffer capacity: is the number of gram equivalents in an acid or base that changes the pH of 1 liter buffer by 1 unit. Maximum buffer capacity occurs when pH = pKa. Higher concentration of buffer constituents buffer capacity due to the acid or base reserve.
Determination of shelf life. Its affected by storage temperature. Preparation is considered fit if it varies from nominal concentration by no more than 65% provided the decomposition products are not more toxic. Stability testing at 4 C and room temperature (22C). Rate of decomposition is determined. Temperatureccelerated stability is also conducted. Arrhenius equation can be used. T90% can also be calculated.
Miscellaneous solutions
Aromatic waters: are saturated aqueous solutions of volatile oils. Used for flavoring. Stored in tight, light resistant containers. Adding large amount of water soluble drug may cause insoluble layer to form (salting out) due to better attraction with the water solvent than the oils. Spirits (essences): volatile substances in 50-90% alcohol. It water is added, oils separate. Used medicinally or as flavors. Store in tight containers. Tinctures: stable alcohol solutions of chemicals or soluble constituents or vegetable drugs. Prepared using extraction using maceration or percolation. Alcohol content varies widely. Fluidextracts: liquid extracts of vegetable drugs that contain alcohol as a solvent, preservative, or both. Prepared by percolation. Ten times as concentrated and potent as tinctures (100% vs. 10%). Mouthwashes: use alcohol or glycerin to dissolve volatile ingredients. Astringents: locally applied solutions that ppt protein. Astringents cell permeability without causing injury. They cause constriction, wrinkling and blanching of skin. They secretions and are used as antiperspirants. Examples: alulminum acetate, aluminum subacetate, calcium hydroxide. Antibacterial topical solutions: e.g. benzalkonium chloride, strong iodine, providone-iodine. .
Suspensions
Magmas: suspensions of finely divided material in a small amount of water. Drugs may be packed dry to avoid instability in aqueous dispersions. Advantages:
Sustained effect: requires dissolution or diffusion step. Stability: drug degradation is slower than in a solution. Taste: for insoluble drugs used in suspension. Solubility: when solvent is not available. Example: only water can be used in ophthalmics, but suspension offer an alternative. Preparation: first solids are wetted by levigation (addition of nonsolvent levigating agent to solid material to form a paste). A surfactant can be used. Then suspending agent is added as aqueous dispersion by geometric dilution. Suspending agents: A. Hydrophilic colloids viscosity by binding with water. Support microbial growth and require preservation. Mostly anionic, except methyl cellulose (neutral) and chitosan (cationic), therefore incompatible with quaternary antimicrobials. Insoluble in alcohol. Acacia: used as 35% water dispersion (mucilage). Neutral pH. Tragacanth: 6% mucilage (less needed). Methyl cellulose: heat and light stable polymer. Soluble in cold but not hot water. Prepared using boiling water. Carboxy methyl cellulose: anionic and water soluble. B. Clays Anionic silicates. Strongly hydrated and exhibit thixotropy. Examples: bentonite (5% magma), veegum.
Emulsions
Advantages: Solubility: e.g. oil soluble drug in aqueous formulation. Stability: usually better than in aqueous solution. Drug action: as in IM injections. Taste: oil soluble drug hidden in aqueous outer phase. Appearance: as in oily material for topical application. Phases of emulsions: most are 2-phases. Internal phase (dispersed or discontinuous phase) in an external phase (dispersion medium or continuous phase). Type of emulsion is determined by relative phase volumes and emulsifying agent used (more important). Maximum volume of internal phase is 74%. Emulsifying agents: lower interfacial tension and form a film at the interface. Natural emulsifying agents: see hydrophilic colloids under suspending agents (acacia, tragacanth, celluloses). Also pectin, gelatin and agar. Agar: viscosity. Gelatin: 1%, can be anionic or cationic. Preparation methods: 1. Wet gum (English) method: emulsion of fixed oil, water, acacia. Make mucilage of water and acacia, then add oil gradually. 2. Dry gum (Continental) method: emulsion of fixed oil, water, acacia. Fixed oil added to acacia and then water is all added at once followed by rapid titration. Electrolyte in high concentration can break the emulsion. Add last. Alcohol can dehydrate and ppt hyrocolloids. Use in concentration. 3. Bottle method: similar to dry method. Used for volatile oils. 4. Nascent soap method: by mixing equal portions of oil and alkali solution to form soap, which acts as an emulsifying agent. Example: olive oil (contains oleic acid, free fatty acid) and lime water calcium oleate for calamine lotion. The drug can be added after emulsion formation if it is soluble in the external phase. If drug is soluble in internal phase, it should be dissolved first during emulsion formation. Synthetic emulsifying agents: 1. Anionic: Soaps form w/o except alkali soap. Examples: SLS 2. Cationic: e.g. benzalkonium chloride. Incompatible with soap. 3. Nonionic: Spans (sorbitan esters, HLB) for w/o, Tweens (polysorbates, HLB) for o/w
Ointments
Used as emollients (make skin more pliable), protective barriers, or vehicles for drugs. Ointment bases 1. Oleaginous bases: not washable. Petrolatum: occlusive, does not rancid, use wax to viscosity. Synthetic esters: e.g. glyceryl monostearate, isopropyl myristate, butyl palmitate, PEG, long chain alcohols. Lanolins: e.g. lanolin oil and hydrogenated lanolin.
2. Absorption bases: anhydrous, water-insoluble, not washable, but can absorb water. Example: anhydrous lanolin (wool fat), hydrophilic petrolatum (petrolatum, bees wax, stearyl alcohol, cholesterol), e.g. Aquaphor. 3. Emulsion bases: Hydrous wool fat (lanolin): w/o with 25% water, emollient and occlusive. Cold cream: w/o with almond oil, white wax, sodium borate. Vanishing cream: o/w with water and humectants (PEG, glycerin). Hydrophilic ointment: o/w with SLS. 4. Water soluble bases: washable and absorb water. PEG ointment: PEG 400 and 4000 by fusion method. PG and PG-alcohol: forms clear gel with 2% hydroxypropyl cellulose. Preparation: metal spatula may interact with iodine or mercuric salts. Use levigation or fusion method. Fusion method: used for solids with melting point. Oil phase melted with highest melting point materials first. Heat water soluble ingredient separately to above the highest melting point. Mixed the two phases in the appropriate order for o/w or w/o.
Suppositories
Used for local (hemorrhoids, infection) or systemic effect. Systemic effect bypasses the first pass metabolism Used when oral route is not possible, e.g., infants, nausea, vomiting, GI distress, coma, debilitation. Types of suppositories: 1. Rectal: cylindrical, tapered bullet like. Adult: 2 g. 2. Vaginal: oval, 5 g, for antiseptics, contraceptives, anti-infective. 3. Urethral: long (6 cm), tapered, local anti-infective. Suppository bases: Minimum 30 C narrow, sharp melting point. Oil soluble drug has mucous absorption from an oil base, and vice versa. Bases that melt: cocoa butter (theobroma oil), witepsol (saturated fatty acid mixture), wecobee (from coconut oil); or Bases that dissolve: PEG. Preparation: the suppository is molded with the fingers after a plastic mass is formed. 1. Hand-rolling: Correct the amount of base needed based on the quantity of the drug and density of the base. 2. Compression: Mixture is placed into compression device. Pressure is applied and mixture is forced into lubricated mold cavities. Used with cocoa butter. 3. Fusion (molds): most common. Use mineral oil to lubricate mold. Pour melt continuously to avoid layering. Avoid for thermolabile drugs and insoluble powders (settle).
Powders
Advantages: compounding flexibility, chemical stability, rapid ingredients dispersion. Disadvantages: time consuming preparation, inaccurate dosing, unsuitable for bad taste / hygroscopic drugs. Milling: mechanical process of reducing particle size (comminution). Micrometrics: is the study of particles. Advantages of milling: surface area dissolution rate and bioavailability (e.g. griseofulvin), drying of wet masses. ointment texture / stability / appearance. uniform distribution of colorants. Particles of same size mixing, segregation. Disadvantages of milling: may change polymorphic form activity. heat / adsorption degradation. Flow problems and segregation. static charge particle aggregation / dissolution. surface area air adsorption / wettability. Comminution techniques: Trituration reducing particle size or mixing with a mortar and pestle. Pulverization by intervention a solvent is added to help pulverization and then evaporated (e.g. alcohol to camphor). Used with gummy substances that reagglomerate or resist grinding. Levigation add a nonsolvent (levigating agent, e.g., mineral oil) to form a past and help pulverization in mortar and pestle or ointment slab and spatula. Avoid gritty feel of solids. Mixing powders: Spatulation: using spatula to mix small amounts of powder on paper or pill tile. Not possible for potent drugs or large quantities. Useful to eutectic mixtures (mixture melting point is lower than each ingredient), such as phenol, camphor, menthol, thymol, aspirin, phenyl salicylate, phenacetin. Inert diluent can be used to minimize contact (MgO, MgCO3, kaolin, starch). Trituration: used both to comminute and mix. For comminution, use porcelain or ceramic mortar with rough surface. For mixing, colorants and easy cleaning, use glass mortar.
Geometric dilution: used for mixing potent drugs with large amount of diluent. First mix equal amounts of drug and diluent in a mortar by trituration, repeat until diluent is used up. Sifting: powders are passed through sifters similar to flour sifters, resulting in a light fluffy product. Not suitable for potent drugs. Tumbling: mix powders in a large container rotated by motor. Use and packaging of powders: As bulk powders or divided powders. For bulk powders, a perforated sifter can is used for external dusting or an aerosol container is used for spraying onto skin. Powders dispensed in bulk: antacids and laxatives (e.g. PEG is mixed with a drink). Douches are mixed with water and applied vaginally. Dentifrices and dental cleansing powders. Powders for ear, nose, throat, tooth sockets, vagina. Non potent substances. Divided powders: dispensed usually in folded paper (chartulae). If drug is not potent, approximate portions by block and divide method (do not weight). Special problems: volatile substances (camphor, menthol, essential oils) use sealed containers. Liquids added to divided powders in small amounts. Hygroscopic substances become moist divide, add diluent, double wrap. Eutectic mixtures.
Capsules
Hard gelatin capsules Storage: contain 15% water, so when humidity capsules become brittle, when humidity capsules become shapeless. Size: empty capsules are numbered (000 largest / 600 mg, 5 smallest / 30 mg). Large capsules are for veterinary use. May add lubricant to flow or wetting agent to dissolution. Filling: by the punch method. Powder is placed on paper and the capsule is pressed into powder until filled. Soft gelatin capsules Preparation: from gelatin shells. Glycerin or polyhydric alcohol (sorbitol) is added to make shells more elastic. Contain preservatives (sorbic acid, parabens). Uniformity and disintegration Uniformity is demonstrated by weight variation or content uniformity. Disintegration are usually not requires unless they are enteric coated. Contents may be designed for sprinkling on food (e.g. Theo-Dur Sprinkle).
Tablets
Advantages of solid dosage forms: accurate dose, easy shipping / handling, less shelf space, no preservative, no taste masking problems, more stable / longer expiration. Advantages of liquid dosage forms: more effective (antacids, adsorbents), easier to swallow. Advantages of tablets; precise dose, content variability, manufacturing cost, easy packaging and shipping, easy to identify, easy to swallow, specific release forms, stable, tamperproof. Disadvantages of tablets: difficult compression, difficult formulation / bioavailability (poor wetting, dissolution, dose). Ideal tablet: free of defects, strong / durable, stable, predictable drug release. Tablet design and formulation (excipients) Diluents: fillers to make up the tablet bulk of dose drugs. May cohesion, flow, or direct compression. Examples: kaolin, lactose, mannitol, sugar, starch, microcrystalline cellulose, calcium phosphate. Do not use calcium salts with tetracycline ( absorption). Binders / adhesives: added dry or liquid to granulation or direct compression. Examples: cornstarch, glucose, molasses, natural gum (acacia, may be contaminated), celluloses (methylcellulose, CMC, microcrystalline cellulose), gelatins, provide (PVP). Liquid binders are more effective. too hard, dissolution. soft crumbling tablets. Disintegrants: disintegration on gastric fluid contact (critical for dissolution and bioavailability). They draw water to tablet, swell and burst. Examples: cornstarch, potato starch, sodium starch glycolate, celluloses (sodium CMC), clays (veegum, bentonite), cation exchange resins.
A portion can be added with the diluent and another with the lubricant after granulation double disintegration. Lubricants / antiadherents / glidants: lubricants friction between tablet and die upon ejection (talc, magnesium stearate, calcium stearate). Anti-adherents sticking, adhesion of granules to the punches or die. Glidants particle friction powder / granule flow. Colors / dyes: disguise off-color drugs, product ID. FDC dyes are applied in solution. Lakes are dyes absorbed on a hydrous oxide (dry powder). Flavoring agents: only for chewable or mouth dissolving tablets. Flavor oils or powders are stable, water soluble flavor are stable. Maximum: 0.75%. Artificial sweeteners: only for chewable or mouth dissolving tablets. May come with diluent (mannitol, lactose). Other agents; saccharin, aspartame. Adsorbents: hold fluid in apparently dry state. Example: magnesium oxide, magnesium carbonate, bentonite. Tablet types and classes For oral ingestion: May be mask taste, color, odor, control release, enteric coating, incorporate another drug, avoid incompatibility, appearance. Compressed: from powders, crystals or granules with or without excipients. No coating. Multiple compressed: layered compress tablet granules around previously compressed granules, then repeat. Compression coated / dry coated made by feeding previously compressed tablet to a machine that compresses an shell around it separate incompatible drugs, provide repeat action / prolonged action. Repeat-action: multiple compressed tablet where the outer shell rapidly disintegrates in the stomach. Example: Repetabs, Extentabs. The components of the inner layer are insoluble in the stomach but soluble in the intestine. Delayed action / enteric coated: delays drug release to prevent stomach destruction, prevent stomach irritation, or better stomach absorption. Enteric: intact in stomach, release in intestine (e.g. Ecotrin). Sugar / chocolate-coated: to protect drug from air / humidity, mask taste / odor. Process includes seal coating (waterproofing), subcoating, syrup coating (for smoothing, coloring), polishing. Disadvantage: time consuming, require expertise, bulky coats. Film coated: compressed tablets coated with water soluble or insoluble polymer (HPMC, povidone, PEG). Film is colored, durable, chipping, bulky (3% wt ), time consuming than sugar coating. May contain film former, plasticizer, surfactant, opacifier, sweetner, color, flavor, glossant, volatile solvent. Air-suspension coated: fed into vertical cylinder and supported by air column (Wurster process) where the coating solution is applied. Chewable: disintegrate rapidly when showed or dissolved. Contains flavored and colored mannitol. Used for children, multivitamins, antacids, antibiotics. Used in oral cavity Buccal / sublingual: allow absorption through oral mucosa after dissolution. Avoid gastric destruction or intestinal absorption. Examples: sublingual nitroglycerin, buccal progesterone. Troches / lozenges / dental cones: dissolves slowly in the mouth and provide local effect. Used to prepare solutions: Effervescent: made by compressing granular effervescent salts (citric acid, tartaric acid, sodium bicarbonate) that release CO2 when contacting water. Example: alkalinizing analgesics (Alka-Seltzer, dissolution, absorption). Other tablets to prepare solution: dispensing tabs, hypodermic tabs, tab triturates. Processing problems Capping: separation of the top or bottom crown from main body of tab. Lamination: separation of tab into two or layers. Usually due to air entrapment. Picking: removal of the surface material by a punch. Sticking: adhesion of material to the die wall. Due to excess moisture or melting ingredient. Mottling: unequal color distribution. Due to different color drug vs. excipient or drug degradation.
Tablet evaluation and control General appearance: size, shape, color, odor, taste, surface, texture, physical flaws, consistency, marking legibility. Hardness / friability resistance: Hardness affects dissolution / disintegration. Slow dissolved tabs are harder, vice versa. Hardness tester measure force required to break tab. Friabilators measure weight loss when tabs roll and fall (<1%). Chewable / effervescent tabs are highly friable, require special packaging. Weight variation: USP standards apply to tabs containing >50 mg drug where drug is > 50% of total weight. Content uniformity: USP standards apply if drug <50 mg. Disintegration: USP test is conducted in vitro. Disintegration time: nitroglycerin (2 min), aspirin (5 min), most other drugs (<30 min), buccal tabs (4hr), enteric coated (none in 1 hr is simulated gastric fluid, within 2 hr in simulated intestinal fluid). Dissolution: standards in USP. Increased emphasis on dissolution replaced disintegration for many drugs.
Aerosols
Pressurized dosage forms that deliver drugs topically or systemically with the aid of liquefied or propelled gas (propellant). Valve allows pressurized product to be expelled continuously or intermittently when the actuator is pressed. Dip tube conveys the formulation for the containers bottom to the valve. Metered dose inhalers (MDIs): aerosol systems for systemic or pulmonary delivery. They contain fine drug mist solution or dispersion. 1 Actuation = 1 dose. Propellants: compressed gases (CO2, N2, NO), pressure with time due to head space. Liquefiable gases: saturated hydrocarbons, hydrofluorocarbons, dimethyl ether, chlorofluorocarbons (CFC). CFC are banned now. Advantages: push-button dispensing convenience, stability of closed container (protects from light, moisture, air, microbes), tampering, wide product range. Disadvantage: propellants are environmental hazard.
The immediate dose is coated as a top layer. Osmotic systems: Example: Oros system (Alza) Oral osmotic pump composed of a core tablet and semipermeable coating that has a small hole (0.4 mm) produced by laser beam for drug exit. The system requires only osmotic pressure to be effective and is independent of pH. Drug release rate is controlled by changing surface area, membrane nature, or hole diameter. Ion-exchange resins: Example: biphenamine (amphetamine and dextroamphetamine), lonamin (phentermine), Pennkinetic system. Ion exchange resins are complexed with drugs by passage of a cationic drug solution through a column that contains the resin. The drug is complexed to the resin by replacement of hydrogen atoms. Then the resin-drug complex is washed and tableted. Release is dependent on ionic environment in GI and resin properties ( pH release). Complex formation: Example: hydroxypropyl-beta-cyclodextrin forms a chemical complex slowly dissolves depending on pH. Hydrocolloid systems: Example: Valrelease (SR diazepam) includes hydrodynamically balanced system (HBS). HBS contains a matrix that is dense than gastric acid, so it remains buoyant. Multiple hydrocolloid layers swell when contacting gastric acid and slowly erode releasing the drug.
absorption depends on muscle vascularity, drug lipid solubility / matrix. SC: vasculature slow absorption. Intra-articular: into the joint. Intrathecal: into the spinal cord. Intradermal: into the dermis. Enteral: Buccal / sublingual: allows nonpolar lipid soluble drug absorption, bypassing first pass metabolism. Peroral: most common, convenient, safe. Disadvantages: inconsistent / incomplete absorption (gastric emptying, intestinal motility), GI enzyme digestion, acid pH decomposition, GI irritation, first pass metabolism. Absorption is usually by passive diffusion. Duodenum is the main absorption site (villi / microvilli surface area). Residence time (period of contact) is needed for absorption. Double peak: cimetidine or acetaminophen as immediate release on empty stomach produce two peak plasma level. Rectal: drug in solution (enama) or suppository is placed in the rectum. Drugs absorbed in the lower 2/3 bypass the liver first pass metabolism. Respiratory: Intranasal: as spray or drops for local (decongestant, steroid) or systemic effect. Pulmonary: inhaled perorally (nebulizer, MDE) into pulmonary tree. Particles > 60 um deposit on trachea. Particles > 20 um do not reach bronchioles. Particles 2-6 um reach alveolar ducts. Particles 1-2 um retained in the alveoli. Particles < 0.6 um exhaled, not deposited. Transdermal (percutaneous): suitable for small lipid soluble molecules (clonidine, nitroglycerin, fentanyl, scopolamine, testosterone, estradiol). Local activity: topical antibiotics, anti-infectives, antifungals, loacal anesthetics. Minimum systemic absorption.
Biopharmaceutical principles
Physicochemical properties
Drug dissolution: bioavailability rate limiting step for drugs with limited solubility. Diffusion is described by Noyes Whitney equation (similar to Ficks law). Drug solubility in a saturated solution is a static equilibrium property. Dissolution rate is a dynamic property with a rate. Particle size / surface area: inversely related. surface area dissolution rate. For some hydrophobic drugs, particle size aggregation to surface free energy. To prevent aggregate formation, small particles are molecularly dispersed in PEG, PVP (povidone), dextrose. Examples: Griseofluvin molecular dissolution in water soluble carrier (PEG 400) bioavailability. Partition coefficient: ratio of solubility at equilibrium in nonaqueous solvent (n-octanol) to that in aqueous solvent (water). Hydrophilic drugs ( water solubility) dissolution. Ionization: ionized form is more polar and more water soluble. Based on Henerson-Hasselbalch equation. Salt formation: type of salt affects dissolution, bioavailability, duration of action, stability, irritation, toxicity. Soluble salt may be stable than nonionized form (e.g. sodium aspirin vs. aspirin). Effervescent forms: contains acid drug and sodium bicarobnate, tartaric acid, citric acid. Water is added prior to use. Excess sodium bicarbonate forms an alkaline solution in which the drug dissolves. CO2 is formed by the decomposition of carbonic acid. For weak acids, potassium and sodium salts are more soluble than polyvalent cation salts. For weak bases, common water soluble salts include hydrochloride, sulfate, citrate, gluconate. Polymorphism: ability to exist in > 1 crystalline form. Polymorphs have different physical properties. Amorphous non-crystalline forms have dissolution. Chirality: drug exists as optically active stereoisomers or enantiomers different PK / PD. Most chiral drugs are used as racemic mixtures. Example: ibuprofen has R and S enantiomers, only S is active. Hydrates: drug may exist in hydrated, solvated form and anhydrous form. Anhydrous ampicillin dissolves faster than hydrated ampicillin. Complex formation: Chelates are complexes involving a ring-like structure and a metal. Natural chelates: hemoglobin, cyanocobalamin, insulin). Tetracycline forms a chelate with polyvalent metal ions water solubility absorption. Many drugs adsorb strongly on charcoal or clay (kaolin, bentonite) by forming complexes. Theophylline + ethylene diamine water soluble complex (aminophylline). Many drugs are complexed with cyclodextrins to solubility. Large drug complexes (drug-protein) do not cross cell membranes easily free drug must first dissociate for absorption or glomerular filtration.
Solutions: are homogeneous mixtures of solutes dispersed molecularly in a dissolving medium. Aqueous solution is the most bioavailable and consistent form (no dissolution). Oral solutions are used as reference preparations for solid oral forms. Elixir (drug dissolved in hydroalcoholic solution) has bioavailability. Alcohol solubility. However, drug may ppt when elixir is diluted in the GI with food, but absorption is still rapid because of surface area. A viscous drug solution (syrup) may mixing, dilution and GI gastric emptying. Suspensions: bioavailability from suspension is similar to solutions due to surface area. Suspending agents: hydrophilic colloids (celluloses, acacia, xantham gum). viscosity may have issues as syrups above. Capsules: Hard gelatin caps are simple (contain powders) and preferred new drugs early clinical trials. Soft gelatin caps contain nonaqueous solution, suspension or powder. It may have bioavailability if water miscible vehicle is used (e.g. lanoxicaps), and vice versa. Aging and storage may affect gelatin shell moisture content and bioavailability. Compressed tablets: ratio of excipients : drug possiblity of excipients affecting bioavailability. Lubricants are usually hydrophobic, water-insoluble drug surface wetting dissolution and bioavailability. Surfactants dissolution and bioavailability. Modified release dosage forms: products that alter the rate or timing of drug release. More stringent quality control is used. Dose dumping, abrupt drug release, is a problem. Allows in dosing frequency. They provide more flat consistent plasma concentration that avoids toxicity and lack of efficacy. A loading dose may be used. Delayed release control the timing of release, e.g. enteric coating. Transdermals: have occlusive backing film to prevent TEWL to hydration and permeation. Concentration gradient is maintained by a drug reservoir. Targeted drug delivery: place the drug at or near the receptor (e.g. specific cell such as tumor, organ, tissue). Systems include macromolecular drug carriers (proteins), liposomes, nanoparticles, monoclonal antibodies. Inserts and implants: drug is impregnated into a biodegradable material and released slowly. Inserted into vaginal, buccal cavity, skin. Example: l-norgestrol implant is inserted in the upper arm for 5-year contraception.
6. Basic Pharmacokinetics
Introduction
Rates and orders of reactions
Reaction rate: velocity of the reaction Reaction order: way in which the drug (reactant) affects the rate Zero order reaction: drug concentration changes with time at a constant rate. Rate constant = Ko (concentration / time; mg/ml/hr). Linear correlation of concentration vs. time with slope=Ko and intercept = Co. First order reaction: change of concentration with time is the product of the rate constant and concentration of the remaining drug. Drug concentration decreases by a fixed percent in each time unit. Linear correlation of log concentration with time. Rate constant )K) = 1/hour. Half life t1/2=0.693/k.
Drug distribution
Drugs distribute quickly to tissues with blood flow Drug cross capillaries by passive diffusion and hydrostatic pressure. Drugs easily cross the capillaries of the kidney glomerulus. Brain capillaries are surrounded by glial cells forming a thick lipid membrane (BBB) diffusion of polar and ionic hydrophilic drugs. Tissue accumulation due to drug/tissue physicochemical or affinity. Lipid soluble drug accumulate in adipose (fat) tissue
Tetracycline accumulate in bone (calcium Complexation). Plasma protein binding: results in a big complex cant cross membranes. Albumin: major plasma protein for drug binding. Alpha1-glycoprotein: binds basic drugs (e.g. propranolol) in the plasma. bound drugs (e.g. phenytoin) can be displaced by other bound drug free unbound drug in effect / toxicity.
One-compartment model
Intravenous bolus injection
Very rapid drug entry. Rate of absorption is negligible. Entire body is one compartment all tissue equilibrate rapidly. Drug elimination: first order kinetics. Elimination rate constant = renal excretion rate constant + metabolism (biotransformation) rate constant Some controlled release oral drugs have zero absorption rate constant. Apparent volume of distribution (Vd): hypothetical volume of body fluid in which drug is dissolved. Vd is needed to estimate amount of drug in the body (Db) relative to concentration in plasma (Cp). Cp = Db / Vd More drug distribution into tissues Cp Vd
Intravenous infusion
Absorption: zero order. Elimination: first order (when infusion stops) Steady state concentration (Css): target plateau drug concentration where fraction of drug absorbed = fraction of drug eliminated. Loading dose (DL): initial IV bolus dose to produce Css as rapidly as possible. Start IV infusion at the same time. DL: amount of drug that, when dissolved in the apparent Vd, produces the desired Dss. Reaching 07% of Css without DL takes ~ t1/2. Time to reach Css depends on the drug elimination half life. IV infusion: ideal for drugs with narrow therapeutic window (controls Cp).
Multiple doses
Drug is given intermittently in multiple-dose regimen for continuous or prolonged therapeutic activity to treat chronic disease. Give new dose before previous dose completely eliminated Cp accumulation to Css. At steady state: Cp fluctuations between a max and a min (C min-max). Superposition principle: assumes that previous drug doses have no effect on subsequent doses total Cp = cumulative residual Cp from each previous dose. Dosing rate = dose size (Do) / dose interval (e.g. X mg/hr). Same dosing rate same average Css but may be different (C min-max). Some AB multiple rapid IV bolus injections. Oral immediate release drug products (multiple doses) rapid absorption, slow elimination. Maintenance dose (DM): after loading dose to maintain Cp at Css. If DM dosing interaval = elimination t1/2 DL = 2 x DM
Multi-compartment models
Drug distributes into different tissue groups at different rates. Tissues with blood flow equilibrate rapidly with the drug. Two-compartment model (IV bolus): First, rapid distribution into highly perfused tissue (central compartment) rapid decline in Cp (distribution phase). Both are first-order processes. Then, slow distribution into peripheral tissues (tissue compartment) slow decline in Cp after equilibration (elimination phase). Vd = Vd at steady state + central + tissue compartment volumes. Two-compartment model (oral): two-compartment ONLY if absorption is rapid but distribution is slow. Models with additional compartments: example of a third compartment: deep tissue space. If frequent interval dosing third compartment accumulation. Elimination rate constant: two constants; one for elimination from central compartment, the other for elimination after complete distribution.
Nonlinear pharmacokinetics
Also known as capacity-limited, dose-dependent, or saturation PK. Result from the saturation of an enzyme of carrier-mediated system. Do not follow first-order kinetics as the dose . AUC or drug excreted in urine are not proportional to dose Elimination t1/2 may at doses. Michaelis-Menten equation: describe velocity of enzyme reactions in nonlinear PK. It described rate of change of Cp after IV bolus. If Cp is the equation is a zero-order rate of elimination. If Cp is first-order. Note that first-order PK = linear PK
Clearance
Total body clearance (ClT)
ClT = drug elimination rate / Cp = K x Vd ClT and Vd are independent variables. T1/2 is a dependent variable. A constant volume of the Vd is cleared from the body per unit time. First order PK: ClT = renal clearance + non-renal (hepatic) clearance ClT t1/2. Vd t1/2
Hepatic clearance
Volume of drug-containing plasma cleared by the liver per unit time. Measurement of hepatic clearance (ClH) Main mechanism for non-renal clearance. Measured indirectly (difference between total and renal clearance). ClH = hepatic blood flow x extraction ratio. Extraction ratio: drug fraction irreversibly removed by an organ or tissue as the drug-containing plasma perfuses the tissue. Blood flow, intrinsic clearance, protein binding All these factors affect hepatic clearance. Blood flow: to the liver is ~ 1.5 L/min. After oral GI absorption to mesenteric vessels to hepatic portal vein through the liver to hepatic vein to systemic circulation. Intrinsic clearance: ability of the liver to remove the drug independent of blood flow due to inherent ability of the biotransformation enzymes (oxidases) to metabolize the drug as it enters the liver. This is affected by enzyme inducers (Phenobarbital, tobacco) and inhibitors (cimetidine, lead). Protein binding: bound drugs are not easily cleared by the liver or kidney. Only free drug crosses the membrane into the tissue and is available to metabolizing enzymes. Biliary drug excretion Active transport (secretion) process. Separate systems for weak acids and weak bases. Excretes MWt drugs (>500) or polar drugs (digoxin, reserpine, glucuronide conjugates). Drugs may be recycled by enterohepatic circulation. GI absorption mesenteric vessels hepatic portal veins liver secrete to the bile store in gallbladder empty into the GI through the bile duct (recirculation). First pass effect (pre-systemic elimination) Portion of oral drugs may be eliminated before systemic absorption due to rapid drug biotransformation by liver enzymes. Measure absolute bioavailability (F). If F < 1 some drug was eliminated before systemic absorption. Common for drug with high liver extraction ratio. If first-pass effect dose (e.g. propranolol, penicillin), different route (e.g. nitroglycerin, insulin), or modified dosage form (e.g. mesalamine).
Non-compartment models
Some PK parameters can be estimated with non-compartment methods using comparison of the AUCs. Mean residence time (MRT): average time for the drug molecules to reside in the body. Called Mean Transit Time or Sojourn Time. It depends on the route of administration. Assumes elimination from the central compartment. MRT = total residence time of all drug molecules in the body / total number of drug molecules. Mean absorption time (MAT): difference between MRT and MRTIV and an extravascular route. Clearance: volume of plasma cleared of the drug per unit time. Steady-state volume of distribution (Vss): amount of drug in the body at steady sate and the average steady-state drug concentration.
Clinical pharmacokinetics
The application of PK principles to the rational design of an individualized dosage regimen. Objectives: maintenance of an optimum drug concentration at the receptor site to produce effect for the desired period, and minimization of SE.
Toxicokinetics
Application of PK principles to the design, conduct, and interpretation of drug sate evaluation studies. Used to validate dose-related exposures in animals in preclinical drug development to predict human toxicity.
Clinical toxicology: study of SE of drugs and poisons. PK in intoxicated patient ( dose) may be very different from a patient taking therapeutic doses.
Population pharmacokinetics
Study of sources and correlation of variability in drug concentration in the target patient population. Includes PK and non-PK parameters such as age, gender, weight, creatinine clearance, concomitant disease.
PK data evaluation
Single dose studies: calculate AUC to last quantifiable concentration, AUC to infinity, Tmax, Cmax, elimination rate constant (K), elimination half life (t1/2). Multiple dos studies: steady state AUC, AUC to last quantifiable concentration, Tmax, Cmax, Cmin, % fluctuation (Cmax-Cmin / Cmin).
Therapeutic substitution
The process of dispensing a therapeutic alternative. For example: dispensing amoxicillin for ampicillin. The substituted drug is usually in the same therapeutic class (e.g. calcium channel blockers) and is expected to have a similar clinical profile.
Formulary issues
A formulary is a list of drugs. Positive formulary: lists all drugs that may be substituted. Negative formulary: lists drugs which cant be substituted. Restrictive formulary: lists only drugs that may be reimbursed without justification by the prescriber. States provide guidance for drug product selection through formulary. FDA annually publishes Approved Drug Products with Therapeutic Equivalence Evaluations (the Orange Book). It is also published in the USP/DI Volume III. Orange Book Codes: A Rated: drug products that are considered therapeutically equivalent. B Rated: drug products that are not considered therapeutically equivalent. AB Rated: products meeting bioequivalence requirements.
Alkanes
Also called paraffins, saturated hydrocarbons. General formula: R-CH2-CH3. Lipid soluble. Common reactions: halogenation, combustion. Chemically inert to air, heat, light, acids, bases. Stable in vivo.
Alkenes
Also called olefins, unsaturated hydrocarbons. General formula: R-CH=CH2. Lipid soluble. Common reactions: addition of hydrogen or halogen, hydration (to form glycols), oxidation (to form peroxides). Volatile alkenes and peroxides may explode in presence of O2 and spark Stable in vivo. Hydration, peroxidation, reduction may occur.
Aromatic hydrocarbons
Based on benzene. Exhibit multicenter bonding. Lipid soluble. Common reactions: halogenation, alkylation, nitration, sulfonation. Chemically stable. In vivo: hydroxylation, diol formation.
Alkyl halides
Halogenated hydrocarbons. General formula: R-CH2-X. Lipid soluble. degree of halogenation Solubility. Common reactions: dehyro-halogenation, nucleophilic substitution. Stable on the shelf. Not readily metabolized in vivo.
Alcohols
Contains OH group. May be primary (R-CH2-OH), secondary (R1/R2-CH-OH), or tertiary (R1/R2/R3-COH). Alcohols are lipid soluble. Low molecular weight alcohols are water soluble. hydrocarbon chain length water solubility.
Common reactions: oxidation, esterification. Oxidation: primary alcohol aldehyde acid. Secondary alcohol ketone. Tertiary alcohol not oxidized. Stable on shelf. In vivo: oxidation, sulfation, glucuronidation.
Phenols
Aromatic compounds containing OH groups directly connected to aromatic ring. Monophenols one OH. Catechols two OH. Phenol (carbolic acid): water soluble. ring substitution water solubility. Most phenols are lipid soluble. Common reactions: with strong bases to form phenoxide ion, esterification with acids, oxidation to form colored quinones. On the shelf: oxidation with air or ferric ions. In vivo: sulfation, glucuronidation, aromatic hydroxylation, o-methylation.
Ethers
General formula: R-O-R. Lipid soluble. Partially water soluble. hydrocarbon chain water solubility. Common reaction: oxidation to form peroxides (may explode). In vivo: o-dealkylation. Stability with size of alkyl group.
Aldehydes
General formula: R-CHO (contains a carbonyl group C=O). Lipid soluble. Low molecular weight aldehytes are also water soluble. Common reactions: oxidation (to acids, in vivo and in vitro) and acetal formation.
Ketones
General formula: R-CO-R (contains a carbonyl group C=O). Lipid soluble. Low molecular weight ketones are also water soluble. Nonreactive and very stable on the shelf. In vivo: some oxidation or reduction.
Amines
Contain an amino group (-NH2). Primary (R-NH2), secondary (R1/R2-NH), tertiary (R1/R2/R3-N), quaternary (R1/R2/R3/R4-N+ X-). Lipid soluble. Low molecular weight amines water solubility. branching water solubility (primary amines and most soluble). Quaternary amines (ionic) and amine salts are water soluble. Common reactions: oxidation (air oxidation on shelf), salt formation with acids. Aromatic amines are basic reactive with acids. In vivo: glucuronidatin, sulfation, methylation. 1ry oxidative deaminatin. 12y/2ry acetylation. 2ry/3ry dealkylation.
Carboxylic acids
General formula: R-COOH (Carboxyl group COOH). Lipid soluble. Low molecular weight acid and Na/K salts water soluble. Common reactions: salt formation with bases, esterification, decarboxylation. Very stable on shelf. In vivo: conjugation (with glucuronic acid, glycine, glutamine), beta oxidation.
Esters
General formula (R-COOR). Lipid soluble. Low molecular weight esters are slightly water soluble. Common reaction: hydrolysis to form carboxylic acid and alcohol (in vivo by esterases / in vitro).
Amides
General formula: R-CONH2 or R-CONR1/R2 (lactam form). Lipid soluble. Low molecular weight amides are slightly water soluble. No common reactions. Very stable on shelf. In vivo: enzymatic hydrolysis by amidases in the liver.
Biochemistry
Amino acid and proteins
Monomeric units of protein (peptide bonds). Formula: NH2-CH-R/-COOH. Proteins are made of 20 AA, differ in R side chain (alpha (C)). Protein hydrolysis to AAs by acids, bases, enzymes. + AA ionize (depending on pH) to zwitterions structure (NH3 -CH-COO /R) water solubility, melting point. Levels of protein structure: primary, secondary (alpha/beta), 3ry, 4ry.
Carbohydrates
Polyhydroxy aldehydes or ketones Monosaccharides: simple single unit sugars, e.g., glucose, fructose. Oligosaccharides: short chains of monosaccharides joined covalently, e.g. sucrose (has to convert into glucose, fructose before GI absorption), maltose (hydrolyzed by maltase into 2x glucose), lactose (milk sugar, has to convert into galactose, glucose before GI absorption). Polysaccharides: long chains of monosaccharides, e.g., cellulose, glycogen.
Biopolymers
Enzymes Linked amino acid chains (proteins) catalysts for biological reactions. They reactions activation energy but do not change reaction equilibrium point, are used up or changed in the reaction. May require cofactors or coenzymes. Cofactor: inorganic (metal ion) or nonprotein organic molecule. Prosthetic group: cofactor firmly bound to apoenzyme (protein portion of a complex enzyme). Coenzymes: organic cofactor that is not firmly bound but actively involved in catalysis. Holoenzyme: complete catalytically active enzyme system. Lyases: removes functional group (deaminase, decarboxylase). Ligases: bind two molecules (e.g. DNA ligase 2 nucleotides). Isomerases: change DL, cistrans, vice versa. Polysaccharides Also called glycans. Long chain polymers of carbohydrates. Homopolysaccharides: Contains one type of monomeric units. Starch plants reserve food, two glucose polymers (linear water soluble amylose, and branched water insoluble amylopectin), enzymatic hydrolysis maltose (glucose disaccharide). Glycogen branched D-glucose chain, polysaccharide storage in animal cells (liver, muscles). Cellulose water soluble, in plant cell wall, linear D-glucose chain, cant be digested (hydrolyzed) by humans.
Heteropolysaccharides: contains two or more monomeric units. Heparin acid mucopolysaccharide with sulfate derivatives, contains glucosamine, in lung tissue, used to prevent clotting. Hyaluronic acid in bacterial cell wall, virteous humor, synovial fluid, contains glucosamine. Nucleic acids Linear polymers of nucleotides pyrimidine and purine bases linked to ribose or deoxyribose sugars (nucleosides) and bound to phosphate groups. Phosphodiester bonds: join successive DNA / RNA nucleotides. DNA: compared to RNA it lacks an OH group and contains T rather than U. (D T, RU). DNA: two complementary alpha helical strands coiled to form double helix. Hydrogen bonding between specific base pairs hold the strands together. Hydrophobic bases are on the inside of the helix. Hydrophilic deoxyribose phosphate on the outside. Backbone: alternating phosphate and pentose units with a purine or pyrimidine attached to each. Strong acids associated with cellular cations and basic proteins (histones, protamines). rRNA (ribosomal): in ribosomes. mRNA (messenger): the template for protein synthesis specifies the polypeptide amino acid sequence. tRNA (transfer): carries activated amino acids to ribosomes for incorporation to the growing polypeptide chain.
Biochemical metabolism
Factors affecting metabolism: substrate concentration, enzymes, allosteric (regulatory) enzymes, hormones, compartmentation. Catabolism: degradation reactions that release energy for useful work (e.g. mechanical, osmotic, biosynthetic). Anabolism: biosynthetic (build-up) reactions that consumer energy to form new biochemical compounds (metabolites). Amphibolic pathways: may be used for anabolic or catabolic purposes. Example: Krebs cycle, it breaks down metabolites to release 90% of the organisms energy, but it also uses metabolites for form compounds such as AA.
Bioenergetics
Substrate level phosphorylation: forms one unit of ATP per unit of metabolite, no oxygen required. Oxidative phosphorylation: forms 2 or more ATP per unit of metabolite. Uses oxidoreductase enzymes (e.g. dehydrogenases) using cofactors NAD (nicotinamide A dinucleotide) or FAD (flavin). Energy released from the reaction is used to form ATP in the mitochondria.
Carbohydrate metabolism
Catabolism: releases energy from carbohydrates. Glycogenolysis: breakdown of glycogen into glucose phosphate in the liver, skeletal muscles controlled by glucagon and epinephrine. Glycolysis: breakdown of sugar phosphates (e.g. glucose, fructose, glycerol) into pyruvate (aerobically) or lactate (anaerobically) to produce energy (ATP) Anabolism: consumes energy to build complex from simple molecules Glycogenesis: formation of glycogen in the liver and muscles from glucose in diet controlled by insulin. Gluconeogenesis: formation of glucose from noncarbohydrate sources (e.g. lactate, pyruvate).
Krebs cycle
Location: in the mitochondria. Absent in RBCs (no mitochondria) Catabolism: converts pyruvate (glycolysis), acetyl CoA (fatty acid degradation) and amino acids into CO2 and water with release of energy. Oxygen dependent (aerobic). Anabolism: forms amino acids (aspartate, glutamate) and heme ring from metabolites. Electron transport: accept electrons and hydrogen from oxidation of Krebs cycle metabolites and couples the energy released to make ATP.
Lipid metabolism
Catabolism Triglycerides stores in fat cells (adipocytes) are hydrolyzed by hormone-sensitive lipases into three fatty acids and glycerol Fatty acids: broken down by beta oxidation to acetyl CoA to Krebs cycle breaks down to CO2, water and energy release. Ketogenesis: very rapid break down of fatty acids leading to formation of ketone bodies (as in DM). Glycerol: enters glycolysis oxidized to pyruvate to Krebs cycle CO2 and water. Steroids: may be converted to bile acids, vitamin D, hormones. Anabolism Fatty acids: formed in the cytoplasm. Unsaturation occurs I the mitochondria or endoplasmic reticulum. Essential fatty acids: linoleic acid (can not be synthesized, diet is only sources). Terpenes: derived from acetyl CoA. Include: cholesterol, steroids, fat soluble vitamins (ADEK), bile acids. Sphingolipids: forms a ceramide backbone with fatty acids. Joins with other compounds to form cerebrosides, sphingomyelin Phosphatidyl compounds: i.e. phosphatidyl choline (lecithin), ethanolamine.
Nitrogen metabolism
Catabolism Amino acids: amino group is removed by transaminase. Carbon skeleton is broken down to acetyl CoA or citric acid derivatives oxidized to CO2 and water for energy. Glycogenic amino acids form glucose as needed by guconeogenesis. Purines: 90% is salvaged, 10% degrade to uric acid using xanthine oxidase. Pyrimidines: breaks down to B-alanine, ammonia, CO2 Anabolism Amino acids: from citric acid cycle intermediates. Essential AA: TIM (threonine, isoleucine, methionine), HALL (histidine, arginine, lysine, leucine), PVT (phenylalanine, valine, tryptophan) PVT TIM HALL Purines / Pyrimidines: from aspartate, carbamoyl phosphate, CO2, other AA.
Nitrogen excretion
Excess nitrogen is toxic must be eliminated, mainly as urea. Urea synthesis: in the liver using the Krebs-Henseleit pathway. Amino acid AA transferases (transaminases) + pyridoxine (vitamin B6) as coenzyme Ammonia + glutamate glutamine + CO2 carbamoyl phosphate urea cycle urea. Uric acid synthesis: most purines are salvages. Remaining purines are excreted as uric acid.
9.
Microbiology
Morphology
Cultural morphology Based on size, shape and texture or colonies grown inj axenic (pure) cultures Each colony originates from a Colony Forming Unit (CFU) consisting of a single cell or group of adherent cells
Stains
Bacteria are small and transparent must be stained to be examined by light microscopy Simple Single dye colors the cells (e.g. gentian violet, safranin) Gram Gram-positive = purple Gram-negative = pink Acid-fast Stains only cells that have an outer layer of a waxy lipid (acid-fast) not those lacking that layer (non acidfast) Spore Heat is used to facilitate the dye entering the spore Capsule Two dyes stain the cell and backgrounds allowing the visualization of the unstained capsular material
Bacilli Cylindrical rod-shaped (pseudomonads, Escherichia) Coccobacilli (combination of small rods or flattened cocci) Spirochetes (helical like a corkscrew) Fusobacteria (tapered ends and slightly curved) Filamentous (organisms are branching) Vibrios (comma shaped) Pleomorphic (exist in varied forms)
Other parameters
Presence or spores, capsules or slime layers Mobility or type of flagella Monotrichous = single flagella at either pole Amphitrichous = flagellum at both poles Lophotrichous = flagella at either or both poles Peritrichous = flagella distributed evenly all around
External structures
Capsule and slime layer Flagella Pili (fimbriae)
Concentration-effect relationship
dose concentration at site of action effect up to a ceiling. Quantal dose-response curve: # of patients exhibiting a defined response by specific drug dose. Bell shaped. Graded dose-response curve: magnitude of drug effect vs. drug dose. Efficacy is measured by the maximum effect. Potency compared different molar doses of different drugs needed to produce the same effect. Log dose-response curve: drug effect vs. log dose. Used to compare efficacy and potency of different drugs with same mechanism of action (same slope). Efficacy; determined by the height of the curve (Emax). Potency: compared using ED50 (dose producing 50% of Emax). Competitive antagonist: parallel shift to the right, same Emax is achieve but at dose. Noncompetitive antagonist: nonparallel shift to the right, lower Emax (action cannot overcome if more agonist is present).
Synthetic products
Drugs synthesized from organic compounds. May have chemical structure resembling active natural products (hydroxymorphone morphine, ampicillin penicillin). Peptidomimetics: molecules with no peptide bonds, molecular weight < 700, activity similar to original peptide (e.g. losartan).
Stereochemistry
Types of stereoisomers: optical, geometric, conformational. Optical isomers: contains at least one chiral (asymmetric) carbon (four different substitutes). Enantiomers: optical isomers that are mirror image of each other, identical physical and chemical properties, potentially different potency, receptor fit, activity, metabolism, etc. One enantiomer rotate the plane of polarized light clockwise (dextro, D, +), the other counter clockwise (Leve, L, -). Example: dextrorphanol narcotic analgesic and antitussive, levorphanl only antitussive. Racemic mixture: equal mixgture of D and L enantiomers, optically inactive. Diastereomers: stereoisomers which are neither mirror image, nor superimposable. Drug must have a minimum of 2 chiral centers. Different physicochemical properties (solubility, volatility, melting point). Epimers: special type of diastereomers, compounds are identical in all aspects except stereochemistry around one chiral center. Epimerization is important for drug degradation and inactivation. Geometric (cis-trans) isomers: occurs due to restricted rotation around a chemical bond (double bond, rigid ring system). Cis-trans are not mirror images, have different physicochemical and pharmacologic properties, because functional groups can be separated by different distances not equal fit to receptors. If functional groups are pharmacophores different biologic activity. Example: cis-diethylstilbestrol has 7% estrogenic activity of trans-diethylstilbestrol. Conformational isomers (rotamers, conformers): non-superimposable molecule orientations due to atoms rotation around single bonds. Common for most drugs, allows drugs to bind to multiple receptors. Example: Ach 2-forms: transmuscarinic, gauche nicotinic Bioisosteres: molecules containing groups that are spatially and electronically equivalent, same physicochemical properties. Isosteric replacement of functional groups alter metabolism potency, SE, activity, duration of action (e.g. procainamide, an amide, has longer duration of action than procaine, an ester). Isosteric analogs: may act as antagonists (e.g. alloxanthine is a xanthine oxidase inhibitor, compared to its isostere, xanthine, the enzyme substrate).
Partial agonists: interact with same receptors but with similar affinity but lower intrinsic activity response. Pharmacologic antagonists: bind to the same receptor as the agonist but with no intrinsic activity. Can be reversible, irreversible, competitive, noncompetitive (like enzyme inhibitors). Chemical antagonists: two compounds react inactivation of both. Example: heparin (acidic polysaccharide) with protamine (basic protein), chelating agents as metal poisoning antidotes (EDTA for calcium / lead, penicillamine for copper, dimercaprol for mercury / gold / arsenic). Functional / physical antagonists: produce antagonistic physiologic actions by binding at separate receptors. Example: acetylcholine, NEp.
Nonspecific action
Form monomolecular layer over entire areas of cells. Large dose is given. Examples: volatile general anesthetic gases (ether, nitrous oxide), some depressants (ethanol, chloral hydrate), antiseptics (phenol, rubbing alcohol).
Cholinergic Hexamethonium, mecamylamine Bretylium, guanethidine Botulinum toxin Amphetamine, tyramine Reserpine Cocaine, desipramine Not a major mechanism Pargyline (MAOAI), selegiline Neostigmine, physostigmine (MAOBI), tolcapone (COMTI) Action conduction velocity, contraction rate /force conduction velocity, contraction rate /force Constricts cerebra, cutaneous, visceral arterioles Dilates skeletal muscle arterioles Iris contracts mydriasis Sphincter / ciliary contraction miosis Relaxes bronchial / tracheal muscles Contraction, secretions Contracts sphincter muscles Relax sphincters, contracts smooth muscles. peristalsis Contracts sphincter motility (perisalsis), relax sphincters, secretions Contraction Relaxation Adipolysis, mobilize fatty acids secretions (eye, sweat, saliva, nasal)
Receptor B1 M Alpha-1 Beta-2 Alpha-1 M Beta-2 M Alpha-1 M Alphabeta Apha-1 M Alpha-1 Beta-2 Beta-3 M
Adrenergic agonists
Direct-acting agonists: Examples: Ep, NEp, terbutaline, dobutamine, naphazoline. Alpha agonist: phenhylephrine. Beta agonist: isoproterenol Catecholamine (Ep, NEp) synthesis: Tyrosinse tyrosine hydroxylase DOPA dopa decaroxylase dopamine dopamine hydroxylase NEp Ep. Catecholamine deactivation: methylation by catechol O-methyltransferase (COMT) and oxidative deamination by monoamine oxidase (MAO). Indirect acting agonists (sympathomimetics): Chemically related to catecholamines. Act by neurotransmitter release. Examples: amphetamine, tyramine, ephedrine. Post-junctional alpha-1: Location: iris, arteries, veins, hair follicle muscles, heart, GI sphincters. Agonist effect: vasoconstriction, smooth muscle contraction. Examples: phenylephrine. Pre-junctional alpha-2: Effect: neurotransmitter release, lipolysis, platelet aggregation. Examples: clonidine, methylnorepinephrine. Beta-1: Location: heart. Effect: force / rate of contraction. Examples: dobutamine. Beta-2: Location: bronchial / vascular smooth muscles. Effect: smooth muscle dilatation / relaxation. Examples: albuterol, terbutaline. Beta-3: Location: fat cells. Effect: lipolysis (for obesity).
Epinephrine: medullary hormone, stimulate all receptors (alpha1-2, beta1-2). Use: treat bronchospasm, hypersensitivity / anaphylactic reactions, duration effect of local anesthetics (SC), restore cardiac activity in cardiac arrest, glaucoma (topically, vasoconstriction aqueous humor production). Norepinephrine: adrenergic neurotransmitter, stimulate alpha1-2, beta-1 (weak beta-2). Phenylephrine: alpha-1 agonist. Use: pressor in hypotensive emergency, duration effect of local anesthetics, nasal decongestion Alpha-1 agonists for nasal decongestion: phenylephrine, oxymetazoline, xylometazoline, phenylpropanolamine Alpha-2 agonists (clonidine, methyldopa, guanfacine, guanabenz): for BP. Clonidine is used to intraocular pressure during surgery. Isoproterenol: beta1-2 agonist, bronchodilator, cardiac stimulant in cardiac shock / arrest. Dobutamine: beta-1 agonist, improve heart function in CHF emergency. Beta-2 agonists (albuterol, terbutaline, metaproterenol): systemic or local bronchodilators for asthma. General SE: arrhythmias, pulmonary hypertension, edema, cerebral hemorrhage, rebound nasal congestion, anxiety.
Adrenergic antagonists
Alpha blockers: include ergotamine, prazosin (alpha-1), phenoxybenzamine (nonselecive, irreversible), tolazoline. Beta blockers: similar structure to beta agonists. Examples: metoprolol (beta-1), propranolol (nonselective). Prazosin (x-azosin): vasodilation for hypertension and BPH symptoms. SE: first dose syncope, de BP, dizziness, drowsiness, palpitation, fluid retention, priapism (continuous penis erection). Phenoxybenzamine / phentolamine: nonselective alpha blockers, treat vasospasm, acute hypertensive emergency (e.g. pheochromocytoma, MAOI, sympathomimetics). SE: BP, tachycardia, ejaculation, miosis, nasal congesion. Tolazoline: for neonatal pulmonary hypertension. Labetolol: alpha-1 and beta1-2 blocker, for hypertension. Propranolol: nonselective beta blocker, for prophylaxis of angina pectoris, ventricular arrhythmias, migraine, for hypertension, heart rate in anxiety and hyperthyroidism. SE: bradycardia, CHF, bronchoconstriction, triglycerides, HDL, depression. Sudden d/c is cadiotoxic. B1 blockers (acetbutolol, metoprolol, atenolol): for hypetension, arrhythmia, angina. For glaucoma: eye drops of timolol (B1-2 blocker) and betaxolol (B1 blocker).
Cholinegic agonists
Nicotinic receptors: Location: at postganglionic neuroeffector sites. Muscarinic receptors: Location: at all autonomic ganglia and at the neuromuscular junction of somatic nervous system. Acetylcholine: endogenous neurotransmitter, very short half life (v. rapid hydrolysis by AChE), ester of acetic acid and choline, very potent. Direct acting agonists: structurally similar to acetylcholine but more resistant to AChE longer duration. Examples: methacholine, bethanecol. Use: non-obstructive urinary retention (bethanechol), glaucoma (pilocarpine, miosis). Indirect acting agonists: most are AChE inhibitors. Reversible inhibitors: most are carbamates (carbamic acid esters), e.g. physostigmine, neostigmine, pyridostigmine. Irreversible inhibitors: organophosphate esters, insecticides, nerve gas, e.g. isoflurophate, echothiophate. Use: glaucoma (miosis), myasthenia gravis, hypercholinergic crisis (neuromuscular junction depolarization blockade), anticholinergic toxicity. General SE: bronchospasm, abdominal cramps, BP, syncope, heart rate, salivation, sweating, lacrimation, miosis, flushing, tremors, diarrhea.
Cholinegic antagonists
Quaternary nitrogen: doesnt pass BBB, e.g. ipratropium, glycopyrrolate, propantheline. Tertiary nitrogen: pass BBB, e.g. benztropine, dicyclomine, pirenzepine, tropicamide. Uses: gland / bronchial secretion before anesthesia (atropine, glycopyrrolate), induce sedation / motion sickness (scopolamine), vagal stimulation of the heart (atropine), produce mydriasis / cycloplegia (homatropine), GI spasms (propantheline), asthma (ipratropium), Parkinsons / extrapyramidal disorders (benztropine, trihexyphenidyl), cholinergic toxicity (atropine).
Ganglionic blockers: e.g. mecamylamine, trimethaphan, for hypertensive crisis. SE: mydriasis, intraocular pressure, blurred vision, dry mouth, constipation, urinary retention, fever, nervousness, drowsiness, dizziness, tachycardia.
Neuromuscular blockers
Nondepolarizing (competitive) drugs
Examples (x-curine, x-curonium, x-curium): curare alkaloids (tubocurarine, metocurine, contain a tertiary amine), and synthetic analogs (atracurium, doxacurium, mivacurium, pancuronium, vecuronium, pipercuronium). Mechanism: compete with ACh for nicotinic receptors at the NMJ end-palate potential depolarization potential not reached. Action is overcome by dose cholinesterase inhibitor. Uses: SE: respiratory paralysis, histamine release, bronchospasm, tachycardia
General anesthetics
Effect: depress CNS and induce reversible state of analgesia, amnesia, unconsciousness, sensory / autonomic reflexes, skeletal muscle relaxation, loss of all sensation. Ideal drug: rapid smooth induction and rapid recovery. General SE: respiratory / CNS / CV depression. Halothane sensitivity to catecholamines.
Local anesthetics
Most are structurally similar to cocaine. Ester drugs: rapid hydrolysis by plasma esterases short action. Examples: cocaine, procaine, chloroprocaine, benzocaine, tetracaine. Amide drugs: longer acting, liver metabolism. Examples: lidocaine, dibucaine, mepivacaine, bupivacaine, etidoacione, prilocaine. (VELD) Mechanism: block Na channels in nerve membrane reversible block of nerve impulse conduction, reversible loss of sensation, no loss of consciousness. At tissue pH lipophilic, uncharged, 2ry or 3ry amine form diffuse through connective tissue and cell membrane enter nerve cells convert to ionized charged ammonium cation active form block generation of action potential remain trapped in cell (ionized cant cross cell membrane).
Epinephrine: mix with local anesthetic vasoconstriction blood flow systemic absorption longer local effect, no systemic toxicity. Use: regional nerve block for pain relief, anesthesia for minor operations, topical anesthesia (dyclonine and pramoxine as throat lozenges and hemorrhoids cream), anesthesia for lower limb / pelvic / obstetric surgery when injected in the epidural. SE: systemic absorption seizures, CNS / respiratory / myocardial depression.
Antipsychotics
Typical (classical) drugs: phenothiazines, thioxanthines (x-othixene, thiothixene, chlorprothixene), butyrophenones (haloperidol). Atypical (newer) drugs: clozapine, risperidone, pimozide, loxapine, molindone, quetapione, sertindole, remoxipride. Advantages: more effective for negative symptoms, extrapyramidal SE. Phenothiazines (x-omazine, x-perazine): chlorpromazine, triflupromazine, prochlorperazine, trifluoperazine, fluphenazine, thioridazine. Fluphenazine esters (decanoate, enanthate) very lipophilic very long acting. Mechanism: block dopamine receptors in the brain (extrapyramidal SE). Other possible effects: H1, alpha1, muscarinic. Atypical drugs: also serotonin antagonism. SE: Central: drowsiness, extrapyramidal (akathesia, dystonia, akinesia, tardive dyskinesia), poikilothermy, appetite, weight gain, release of hormones. Peripheral: postural hypotension, reflex tachycardia, impaired ejaculation, dry mouth, blurred vision, liver toxicity.
Antidepresseants / antimanics
MAO-I: phenelzine, isocarboxazid ( potent), tanylcypromine ( potent). Mechanism: block oxidative deamination of brain biogenic amines (NEp, serotonin). Effect takes 3 weeks. Use: depression, phobic anxiety, narcolepsy, SE use. SE: CNS (stimulation, tremor, agitation, mania, insomnia), BP, anticholinergic SE (constipation, dry mouth, urinary retention). DI: tyramine foods, sympathomimetic drugs (hypertensive crises), TCA: secondary or tertiary amines, x-ipramine, x-triptyline, x-pin (doxepin, amoxapine, dibenzoxazepine). Mechanism: CNS re-reuptake of biogenic amines (Nep, serotonin). Also block beta, serotonin receptors, reuptake. Use: depression, enuresis (bedwetting), obsessive-compulsion, anxiety. SE: CNS (drowsiness, confusion), BP, tachycardia, anticholinergic SE, bone marrow depression, m ania. Atypical antidepressants: bupropion, trazadone, mefazadone, SSRI, venlafaxine. Mechanism: CNS re-reuptake of biogenic amines. SE: similar to TCA + blurred vision, tinnitus, sex dysfunction. Antimanics (mood stabilizers): lithium carbonate, valproic acid, carbamazepine. Mechanism: lithium transmembrane Na exchange, neurotransmitter release, inositol metabolism. Use: manic depression / bipolar disease. SE: lithium causes urination, fine hand tremor ( with time).
Anxiolytics / sedative-hypnotics
Examples: BZD (diazepam, alprozlam, flurazepam, halazepam, oxazepam, prazepam, lorazepam, chlordiazepoxide, clorazepate), buspirone, zolpidem. Old drugs: barbiturates, hydroxyzine no longer used due to risk of tolerance, dependence, withdrawal reactions, and SE ( CNS depression). Diazepam: not basic enough to form water soluble salt with acid dissolve in propylene glycol for IV, may ppt if mixed with water. Barbiturates: derivatives of barbituric acid. Long / branched / unsaturated side chain lipid solubility metabolism, onset, duration of action, potency. Phenobarbital (barbiturates) strong enzyme inducer. Weak acids, in overdose alkalinize the urine excretion. BZD: Mechanism: GABA-ergic, chloride channel opening chloride conduction membrane hyperpolarization. Also CNS depression (hypnotic, anesthetic, anticonvulsant, muscle relaxant, alcohol depression). Use: anxiety, insomnia, pre-anesthesia, during acute alcohol withdrawal. SE: CNS depression, ataxia, confusion, abuse / dependence. Buspirone (x-pirone): Mechanism: bind to central dopamine, serotonin receptors. No CNS depression (hypnosis, anti-convulsion, alcohol interaction, no abuse, no rebound anxiety). Use: anxiolytic (effect takes a week). SE: headache, dizziness. Zolpidem (Ambien): Mechanism: strong sedation but anxiolytic effect (for insomnia). Use: insomnia. No abuse, rebound insomnia, or respiratory depression.
Barbiturate: Mechanism: similar to BZD. Use: Ultra-short acting barbiturates (thiopental): induce anesthesia. Long acting barbiturates (phenobarb): antiepileptics. SE: hypnosis, drowsiness, nystagmus, bradycardia, BP, anemia, liver toxicity, respiratory depression. DI: enzyme induction Chloral hydrate: aldehyde prodrug. Use: induce sleep, pre-anesthesia. SE: toxic active cumulative metabolite, CNS depression, alcohol effect, leukopenia. DI: enzyme induction
Antiepileptics
Older agents: long-acting barbiturates (phenobarb, mephobarb, metharbital, primidone), phenytoin (hydantoin), succinimides (ethosuximide, phensuximide), valproic acid, trimethadione, dimethadione. Newer agents: carbamazepine, BZD (diazepam, clonazepam, clorazepate), gabapentin (GABA analog), lamotrigine, felbamate. Pharmacology: or prevent excessive discharge and spread of excitation from CNS seizure c enter. Phenytoin: Na efflux Barbiturates, BZD, valproic acid: GABA-ergic inhibitory neuronal function. Tonic-clonic (grand mal) carbamazepine, pheytoin, phenobarb. Uses: Status epilepticus diazepam, phenytoin, phenobarb Absence (petit mal) clonazepam, phenobarb, valpric acid Myoclonic clonazepam Partial gabapentin, lamotrigine, flebamate Pscyhomotor carbamazepine, phenytoin, phenobarb General SE: CNS (drowsiness, confusion, diplobia, nystagmus), blood toxicity, allergy, Stevens-Johnson, birth defects (no safe drugs here). Phenytoin: gingival hyperplasia, arrhythmias. IV barbiturates / BZD SE: CV collapse, respiratory depression
14. Autacoids
Autacoids are local autopharmacological agents or local hormones. May also function as neurotransmitters (e.g. histamine, serotonin). Also include leukotrienes (discussed later).
Histamine
Chemistry: bioamine derived from dietary histidine. H1-antagonists: diphenhydramine, dimenhydrinate, doxylamine, clemastine, meclizine, cyclizine, hydroxyzine, cyproheptadine, promethazine, chlorpheniramine, brompheniramine, tripelennamine, pyrilamine. New H1 antagonists (loratadine, desloratadine, fexofenadine, cetirizine, astemazole, acrivastine) are less sedating due to their inability to cross BBB. H2-antagonists: ranitidine, cimetidine, famotidine, nizatidine. Pharmacology: H1-receptors: allergic and anaphylactic responses (bronchoconstriction, vasodilation, spasmodic GI smooth muscle contraction, capillary permeability, itching, pain). H2-receptors: secretion of gastric acid, pepsin, intrinsic factor. Indications: exogenous histamine may be used for diagnosing gastric acid function (not very safe). H1-blockers: allergy symptoms (seasonal rhinitis, conjunctivitis), common cold (rhinovirus) infection, urticaria. Agents with anticholinergic effect (meclizine, cyclizine, dimenhydrinate, diphenhydramine): motion sickness and vertigo nausea and vomiting. Promethazine: antiemetic. Hydoxyzine: mild anxiolytic. H2-blockers: gastric hypersecrtion (ulcers, Zollinger-Ellison, GERD). SE: H1-blockers: CNS (sedation, depression, fatigue, except in new agents), GI upset, anticholinergic (dry mouth, constipation). Non-sedating H1-blockers: arrhythmia, especially with hepatic enzyme inhibitors, grapefruit. H2-blockers: CNS (dizziness, confusion), liver / kidney damage, liver enzyme inhibition (cimetidine), androgenic effects (cimetidine).
Serotonin
Chemistry: serotonin is 5-HT (5-hydroxytryptamine). Bioamine synthesized from tryptophan. Serotonin agonists (triptans): idole derivatives of serotonin. Also cisapride, benzamide, ergot alkaloids (ergonovine, dihydroergotamine, bromocriptine, methylsergide, partial agonists / antagonists). Serotonin antagonists: ondasetron, granisetron.
Pharmacology: Serotonin: vasoconstriction, platelet aggregation, nausea / vomiting, anxiety, depression, appetite, acetylcholine release. Serotonin agonists: Cisapride: releases Ach (treat GERD, off market). Serotonin antagonists: prevents nausea / vomiting. Indications: Agonists: drugs use the serotonin system to affect the CNS and modulate behavior (dexfenfluramine as anorexiant, buspirone as anxiolytic, SSRI for depression). Triptans and ergots are used for migraines. Ergots are used to postpartum hemorrhage (vasoconstriction uterine contraction). Bromocriptine is used to prevent post partum breast enlargement. Antagonists: prevents nausea and vomiting due to cancer chemotherapy. SE: Agonists: dizziness, tight chest, coronary vasoconstriction (CI in angina, BP). Cisapride: arrhythmia, diarrhea. Ergots: cold / ischemic extremities, GI upset. Antagonists: headache, dizziness, constipation.
Prostaglandins
Chemistry: derivatives of prostanoic acid (ringed structure). Membrance phospholipids phospholipase A2 arachidonic acid COX enzyme PG. COX I: protects gastric mucosa (PG), homeostasis (thromboxane synthesis). COX II: expressed only in response to inflammation or injury. PG classification subscripts relates to the number and position of double bonds in the aliphatic chains. Pharmacology: Endogenous: release in response to insults (chemical, bacterial, mechanical). Cause pain and edema. Physiologic responses: PGI: vasodilation, platelet aggregation, gastric release of bicarbonate and mucus (protect epithelium). PGE: platelet aggregation, gastric acid secretion, broncho-relaxation. PGD/PGF: bronchoconstriction. Indications: PGE1 analogs: misoprostol to prevent NSAID induced GI ulcers, alprostadil for impotence due to erectile dysfunction. PGE2 analogs: dinoprostone is abortifacient, for cervical ripening in pregnancy. PGF2alpha analogs: latanoprost topically to intraocular pressure in glaucoma, carboprost is abortifacient (not available in US). PGI analog: epoprostenol treats pulmonary hypertension. SE for PGE: CNS (irritability, fever, seizures, headache), cardiovascular (hypotention, arrhythmia, flushing), respiratory depression, hematologic (anemia, thrombocytopenia), diarrhea, abortion.
16. Endocrinology
Pituitary hormones
Posterior pituitary hormones
Oxytocin: octapeptide. Action: stimulate uterine contraction, induce labor. Use: promote delivery, control postpartum bleeding. SE: uterine spasm / rupture, fetal effects (bradycardia, jaundice), water intoxication / coma. Vasopressin: octapeptide. Action: vasopressor and anti-diuretic. hormone (ADH) activity. It reabsorption of water at distal renal tubules. Use: neurogenic diabetes insipidus, postoperative abdominal distention. SE: GI cramps, vomiting, tremor, sweating, bronchoconstriction.
Menotropin: produce ovarian follicular growth and induce ovulation by FSH and LH-like actions. Use: induce ovulation and pregnancy in anovulatory infertile women, spermatogenesis in men. SE: gynecomastia in men, hypersensitivity, thromboembolism, ovary enlargement.
Gonadal hormones
Estrogen
Estrogen receptors: in the nucleus in the vagina, uterus, mammary glands, anterior pituitary, hypothalamus alter mRNA. Uses: oral contraceptives (with progestins), menopause symptoms, acne, osteoporosis, prostate cancer. SE: edema / fluid retention, weight gain, triglycerides, hypertension, thromboembolism, M I, stroke, GI upset, endometrial cancer. Estradiol: principal estrogenic hormone, in equilibrium with estrone. Estradiol esters are used as IM injections in oil for depot action (valerate, cypionate). The esters hydrolyze slowly in muscle tissue before absorption (prodrugs). Synthetic estrogens: resist first pass metabolism oral efficacy. Examples: ethinyl estradiol, 3methyl ether mestranol (contraceptives), quinestrol (ERT). Non-steroidal synthetic estrogens: e.g. diethylstilbestrol. Estrogen antagonists: e.g. clomiphene, tamoxifen citrate, toremifene citrate. Uses: clomiphene induce ovulation, tamoxifen breast cancer. Aromatase inhibitors: anastrozole, letrozole (non-steroidal) conversion of androgens to estrogens. Use: advanced breast cancer. Selective estrogen receptor modulators (SERM): raloxifene bone resorption, bone turnover. Estrogen effect on bone and lipids but estrogen antagonist effect on uterus and breast. Use: prevention of osteoporosis.
Progestins
Progesterone: C-21 natural steroidal progestin. Synthetic progestins: 17alpha-hydroxyprogesterones, 17alpha-ethinylandrogens. lipid solubility, first pass metabolism, oral effect Mechanism: similar to estrogens (intracellular receptors mRNA). Uses: oral contraceptives (alone or with estrogens), uterine bleeding, dysmenorrhea, endometriosis. SE: irregular period, breakthrough bleading, amenorrhea, weight gain, edema. 17alpha-hydroxyprogesterones: e.g. medroxyprogesterone acetate, megestrol acetate 17alpha-ethinylandrogens: e.g. norethindrone, norgestrel, androgens with progesterone activity. Used as oral contraceptives.
Adrenocorticosteroids
Synthesis: in the adrenal cortex. All steroids have fused reduced 17-carbon-atom ring. Most natural steroids have some mineralo- and gluco- effect. All require cytoplasmic receptors to transfer to the nuclei of target tissue cells.
Uses: replacement therapy (adrenal insufficiency), last resort for severe disabling arthritis, severe allergic reactions, ulcerative colitis, kidney disease, cerebral edema, topical anti-inflammatory. SE: peptic ulcer, GI bleeding, intraocular / intracranial pressure, headache, muscle weakness, skin atrophy, edema, weight gain, excitation, irritability, hypertension, hyperglycemia, osteoporosis, flushing, hirsutism, cushingoid moon face / buffalo hump, immunity, infections. Mineralocorticoids: Na retention, K excretion. Glucocorticoids: anti-inflammatory, protein-catabolic, immunosuppressant. Cortisone / hydrocortisone: natural glucocorticoids. Synthetic and semi-synthetic glucocorticoids try to mineralocorticoid activity. Examples: prednisone, prednisolone, triamcinolone, betamethasone, dexamethasone. Aldosterone: natural mineralocoritoid. Synthetics: fludrocortisone acetate, desoxycoriticosterone acetate.
Antianemic agents
Iron
Iron preparations: ferrous salts are better absorbed from GI than ferric salts. Examples: ferrous sulfate, ferrous gluconate, ferrous fumarate. Iron dextran (IV) = colloidal complex of ferric hydroxide and low molecular weight dextrans. Iron (ferrous salts): easy GI absorption stored in bone marrow, liver, spleen as ferritin and hemosiderin incorporate into hemoglobin iron reversibly binds molecular oxygen. Iron (ferrous salts): iron deficiency anemia (hypochromic, microcytic RBCs poor oxygen transport). Cyanbocobalamin (Vit B12): nucleotide-like macro-molecule. Includes cyanide and cobalt. Iron (ferrous salts): GI distress, constipation, diarrhea, heartburn
Folic acid
Folic acid: structure includes PABA, glutamic acid. Folic acid: easy GI absorption, stored intracellularly. Precursor for several coenzymes (derivatives of tetrahydrofolic acid). Deficiency causes megaloblastic anemia but not neurologic damage. Folic acid: megaloblastic anemia due to folic acid deficiency. Folic acid: rare allergy if taken parenterally.
Thyroid preparations
Action: mimic the activity of endogenous thyroid hormones regulate growth and development, calorigenic and metabolic activity, positive inotropic / chronotropic effects (sensitize beta receptors). Use: hypothyroidism (e.g. Myxedema), Myxedema coma, cretinism, simple goiter, endemic goiter. SE: rare, palpitations, nervousness, insomnia, weight loss. Sodium salts of T4/T3. T4 can be given alone (converts to T3).
Liotrix: 4:1 mixture of levothyroxine sodium to liothyronine sodium, no advantages over levothyroxine only. Thyroid USP: from dried defatted thyroid gland of domestic animals. Standardized based on iodine content. Thyroglobulin: purified extract of frozen porcine or bovine thyroid gland, contains T4 and T3. Thyrotropin (TSH): purified and lyophilized hormone from bovine anterior pituitary. Use: detection and treatment of thyroid cancer. SE: anaphylaxis, urticaria, gland swelling, tachycardia, arrhythmia, GI upset.
Thyroid inhibitors
Use: treat hyperthyroidism (e.g. Graves disease, toxic adenoma). Ionic inhibitors: such as thiocyanate (SCN-) and perchlorate (ClO4-), inorganic monovalent anions concentration of iodide by the thyroid. Use: rarely use as drugs, but metabolism of foods (e.g. cabbage) and drugs (e.g. nitroprusside) can produce excess SCN-. concentration iodides: such as Lugols solution, iodides their own transport, synthesis of mediators, hormone release. Use: before thyroid surgery to make gland firmed and its size. SE: Iodism ( salivation, skin rashes, eyelid swelling, sore gum/teeth/larynx/pharynx). 131 Radioactive iodine ( I) sodium: trapped by thyroid gland incorporated into tyrosine / thyroid hormone. Radioactive beta particles local destruction of thyroid cells. SE: delayed hypothyroidism. 131 Thiourylenes: thyroid synthesis. Examples: propylthiouracil, methimazole. Use: with I to control mild hyperthyroidism. SE: urticaria, dermatitis, blood toxicity, joint pain / stiffness.
Biotransformation pathways
Phase I reactions
Polar functional groups are introduced to the molecule, or unmasked by oxidation, reduction, hydrolysis. Oxidation: Most common reaction. Mostly in the liver. Catalyzed by cytochrome P450. Cytochrome P450: oxidases, bound to smooth endoplasmic reticulum, require NADH, exist in multiple isoforms (CYP11Ax, CYP17By, etc) large # of substrates. Involved in metabolism or bile acids, steroids, xenobiotics / drugs. Oxidized drug polarity / water solubility tubular reabsorption urine excretion. Reduction Same goal as oxidation ( polarity by reductases). GI bacterial flora azo and nitro reduction reactions. Enzymatic hydrolysis Addition of water across a bond polar metabolites.
Esterase: present in the plasma and tissues, nonspecific, hydrolyzes esters to alcohol and acid, responsible for activation of many prodrugs. Example: procaine. Amidase: hydrolyze amides into amines and acid (deamidation) in the liver. Example: procainamide.
Phase II reactions
Functional groups of the original drug or a phase I metabolite are masked by a conjugation reaction polar metabolites excretion, no crossing of cell membranes (pharmacologically inactive, no toxicity). Conjugation reactions: combine parent drug (or metabolite) with certain natural endogenous constituents (glucuronic acid, glutamine, glycine, sulfate, glutathione). Requires high energy molecule and an enzyme. High energy molecule: consist of coenzyme bound to endogenous substrate, parent drug, or metabolite. Enzyme: called transferases, found in the liver and catalyze the reaction. Glucuronidation: most common conjugation pathway due to large supply of glucuronic acid (high energy form reacts using glucuronyl transferase). Common with OH group (form ethers) and COOh group (form esters). Reaction adds 3-OH groups and 1-COOH group hydrophilicity. Glucuronides with MWt bile excretion to intestines intestinal beta-glucuronidase hydrolyze the conjugate reabsorption. Sulfate conjugation: using sulfo-transferase. Amino acid conjugation: reaction of glycine or glutamine with aliphatic or aromatic acids to form amides using N-acyltransferase. Glutathione conjugation: very critical for preventing toxicity from harmful electrophilic agents (halides, epoxides). Glutathione (tripeptide) + electrophile + glutathione S-transferase mercapturic acid. Methylation: of oxygen- nitrogen- or sulfer-containing drugs less polar but inactive metabolites. Example: COMT methylates catecholamines such as epinephrine. Acetylation: less polar metabolites with N-acetyl-transferase. Metabolites (e.g. of sulfonamides) may accumulate in the kidney crystalluria / tissue damage.
Genetic variations
Acetylation rate: depends on the amount of N-acetyl-transferase, which depends on genetic factors. Fast acetylators hepatotoxicity from isoniazid. Slow acetylators other isoniazid SE. PM Phenotype: metabolism of B-blockers, antiarrhythmics, opioids, antidepressants.
Drug dosage
dose may saturated metabolic enzymes. As the saturation approaches 100% change from first to zero-order metabolism. When metabolic pathway is saturated > possible alternative pathways. Example: therapeutic APAP doses glucuronic / sulfate conjugation, toxic doses conjugation is saturated N-hydroxylation liver toxicity
Nutritional status
Conjugation agent levels (sulfate, glutathione) is dependent on nutrition
protein diet glycine, oxidative drug metabolism capacity. Diet in essential fatty acids (linoleic acid) synthesis of certain enzymes metabolism of hexobarbital. Diet in minerals (Ca, Mg, Zn) metabolism. Fe metabolism. Diet in vitamins (A, B, C, E): C oxidation. E dealkylation, hydroxylation.
Age
Metabolic enzyme systems are not fully developed at birth doses in infants / children to avoid SE, especially for glucuronide conjugation. Older children liver develops faster than in body weight efficacy. Elderly metabolizing enzymes elimination Cp SE
Gender
Due to androgen, estrogen, adrenocorticoid activity CYP450 isozymes. Example: oxidative metabolism is faster in men.
Administration route
Oral: first-pass effect oral dose IV: by pass first-pass effect dose compared to oral dose. Sublingual / rectal: also bypass first-pass effect. Variable absorption from rectal administration.
Chemical structure
Presence of certain functional groups influences drugs metabolic pathway (route, extent, degree of metabolism).
Circadian rhythm
Nocturnal Cp of theophylline, diazepam are than diurnal Cp.
Extra-hepatic metabolism
Plasma: contains esterases (hydrolyze esters). Simple esters (procaine, succinyl choline) are rapidly hydrolyzed in the blood. Esterases can also activate prodrugs. Intestinal mucosa: microsomal oxidation, conjugation (glucuronide, sulfate) first pass effect of lipid soluble drugs during absorption. Intestinal bacterial flora: secrete metabolizing enzymes. Ulcerative colitis flora. Diarrhea, antibiotics flora. Flora secrete beta glucuronidase hydrolyze polar glururonide conjugates of bile reabsorption of free nonpolar bile acids eneterohepatic circulation. Flora convert vitamin K to active form, and cyclamate (sweetener) to cyclohexylamine (carcinogen). Flora produce azoreductase converts sulfasalazine to 5-aminosalicylic acid (anti-inflammatory) and sulfapyridine (antibacterial). Stomach acidity: degradation of penicillin G, carbenicillin, erythromycin, tetracycline, peptides / proteins (insulin). Nasal mucosa: CYP450 activity and metabolism on nasal decongestants, anesthetics, nicotine, cocaine. Lung: first pass metabolism of IV, IM, transdermal, SC drugs but to degree than the liver. Also, second pass metabolism for drugs leaving the liver. Placenta: if drug is lipid soluble enough to get to circulation pass through the placenta too. Placenta is not a physical or metabolic barrier to xenobiotics. Very little metabolism occurs. Smoking induce certain enzymes in pregnant women carcinogens from polycyclic HC. Fetus: depends on fetal age, glucuronic acid conjugation. Chloramphenical glucuronidation gray baby syndrome. bilirubin glucuronide neonatal hyperbilirubinemia.
Pharmacologic
Levodopa (L-dopa): amino acid precursor of dopamine (for Parkinsons). Unlike dopamine, it can penetrate BBB and reach CNS to be decarboxylated to dopamine. Carbidopa: DOPA decarboxylase inhibitor that does not cross BBB peripheral activation and SE. Beta-lactam AB: use clavulanic acid (a beta-lactamase inhibitor). Ifosfamide: alkylating agent in vivo metabolic activation nitrogen mustard. Acrolein is a byproduct of metabolic activation react with nucleophiles on renal proteins hemorrhagic cystitis. Combine ifosfamide. with mesna (neutralizes acrolein in the kidney).
Chemical
Testosterone: not orally active due to rapid oxidation of 17-OH group. Methyl-testosterone: 17alphamethyl group potent but no rapid first pass metabolic deactivation used orally. Same for estradiol analogs. Tolbutamide: oxidation of para-methyl group rapid deactivation. Chlorpropamide: non-metabolizable para-chloro group long t1/2. Isoproterenol: potent beta agonist for asthma. Rapid metabolism by COMT (catechol) poor oral activity. Metaproterenol: not metabolized by COMT orally active, long t1/2. Octreotide: synthetic octa-peptide severe diarrhea in tumors, SC. It mimics action of somatostatin (14-AA peptide, short t1/2, only IV infusion) but resistant to hydrolysis, proteolysis.
Prodrugs
Require in vivo biotransformation (phase I) to produce activity The following are potential advantages for prodrugs:
water solubility
Useful for ophthalmic and parenteral formulations Example: sodium succinate esters, sodium phosphate esters to make water-soluble steroid prodrugs
lipid solubility
duration of action: estradiol lipid-soluble esters (benzoate, valerate, cypionate) prolonged activity (IM of esters in oil). oral absorption: by converting carboxylic acid groups to esters converted back to active acids by plasma esterases. Example: lipophilic orally absorbed enalapril very potent orally inactive enalaprilat. topical absorption: of steroids by masking hydroxyl groups as esters or acetonides polar dermal permeability. Examples: triamcinolone acetonide, betamethosone valerate, diflorasone diacetete. palatability: sulfisoxazole acetyl (ester, water solubility, ok taste for children) sulfisoxazole (bitter)
GI irritation
NSAIDs ulceration by direct irritant effect of acidic molecules and of gastro -protective PG. Sulindac, nabumetone prodrugs with GI effect
Site specificity
Methyldopa: structurally similar to L-dopa transported to CNS metabolized to active alphamethyldopamine central alpha-2 agonist Omeprazole: activated at acidic pH < 1 inhibition of H+/K+ATPase. Formaldehyde: effective urinary tract antiseptic. Orally toxicity. Methenamine non-toxic prodrug hydrolyzes to formaldehyde and ammonium ions in acidic urine (pH<5.5). Use enteric coating to prevent activation in the stomach. Olsalazine: polar dimer of 5-aminosalisalyic acid poor oral absorption. In large intestine colonic bacteria cleave azo bond free active. Diethylstilbestrol: synthetic estrogen for prostate cancer feminizing SE. Diethylstilbestrol diphosphate (ester prodrug) activated by acid phosphatase in prostate tumor cells local action, systemic SE.
shelf-life
Cefamandole: 2 generation cephalosporin, unstable in solid dosage forms. Cefamandole nafate: stable formic acid ester hydrolyzed by plasma esterases. Cyclophosphamide: stable prodrug in vivo oxidation + nonenzymatic decomposition active phosphoramide mustard.
nd
Drug interactions
Types of interactions: drug-drug, drug-food, drug-chemical, drug-laboratory. Precipitant: drug, food or chemical causing the interaction. Object: drug affected by the interaction. Epinephrine, erythromycin decompose in IV alkaline pH do not mix with aminophylline (alkaline).
PK interactions
Due to in absorption, distribution (protein / tissue binding), elimination (excretion / metabolism).
Absorption
Epinephrine (vasoconstrictor) percutaneous absorption of lidocaine (local anesthetic). CHF GI blood flow drug absorption MAO inhibitors + foods w/ tyramine metabolism hypertensive crisis Antibiotics (erythromycin) intestinal flora digoxin microbial deactivation bioavailability. Antacids / H2 antagonists GI pH ketoconazole dissolution intestinal motility (anticholinergics , laxatives ) absorption Cholestyramine / kaolin digoxin adsorption bioavailability Complexation by divalent cations tetracycline bioavailability
Distribution
Due to in plasma protein binding / displacement or tissue / cellular interactions. Valproic acid displaces phenytoin and its liver metabolism phenytoin. Quinidine displaces digoxin and digoxin clearance digoxin.
Elimination / clearance
Due to in kidney or liver clearance (enzyme induction / inhibition, enzyme substrate competition, blood flow) . Grapefruit juice is a powerful inhibitor of CYP3A4. Enzyme inducers: tobacco (polycyclic aromatic HC), barbiturates, rifampin, carbamazepine, phenytoin, omeprazole, troglitazone. Enzyme inhibitors: cimetidine, ketoconazole, ciprofloxacin, erythromycin, ritonavir / nelfinavir, clopidrogel.
Food-drug interactions
drug absorption. Example: Complexation of tetracycline + calcium
Delayed/ absorption: NSAIDs, APAP, antibiotics, ethanol. absorption: griseoflulvin, metoprolol, phenytoin, propoxyphene
Chemical-drug interactions
Smoking (enzyme induction) clearance of theophylline, BZD, TCA Alcohol: acute use metabolism, chronic use metabolism.
PD interactions
Antagonistic, additive or synergistic effect. Similar action excessive or toxic response. Example: alcohol + antihistamine both CNS depressants, promethazine + antihistamine both anticholinergic. Thiazide diuretic deplete potassium sensitivity to digoxin, deplete sodium lithium toxicity, anticoagulant + aspirin risk of bleeding.
Clinical significance
Not all interactions are dangerous. Interacting drugs can be prescribed under supervision with monitoring. Example: cimetidine with antacids do not take both at the same time Some interactions are good efficacy, SE. Examples: trimethoprim + sulfamethoxazole ( efficacy in UTI), amoxicillin + clavulanate potassium (beta lactamase inhibitor spectrum), hydrochlorothiazide + enalapril (balance potassium), penicillin + probenicid ( tubular secretion, t1/2), saquinavir + food ( absorption). Likelihood: established, probable, suspected, possible, unlikely Consider dose side and duration, interaction onset / severity.
Tertiary (textbooks)
Benefits: easy and convenient access to large number of topics, include background information on drugs / diseases, validity and accuracy of information can be verified by using references. Limitations: may take years to publish information may be outdated, chapter author may not have done a thorough literature search, author may have misrepresented the original article. Considerations: author, publisher, edition, year of publication, scope, presence of bibliography.
Internet
Benefits: expanded searching capabilities, most useful for company specific information, issues currently in the news, alterative medicine, government information. Limitations: may not be peer reviewed or edited, not always reliable (evaluate source).
US Adopted
Names
USP Dictionary Unlisted Drugs USP DI Index Medicus X X X X
Search strategies
Is it a clinical or research-related question? Define as specifically as possible. Identify appropriate index search terms (keywords, descriptors). Determine quantity and quality of needed information. Ascertain as much as possible about the drug and the inquirer. What is the drug indication? Is the drug approved or not? Patient information (age, sex, weight, medical conditions, other drugs, signs of SE, allergies, etc).
Information resources
Computerized databases: Poisindex: CD database updated quarterly and used by poison control centers. TOMES: Toxicologic, Occupational Medicine and Environmental Series info on industrial chemicals. Printed publications: textbooks and manuals are useful but suffer a lag time of information published in primary literature. Internet: Center for Disease Control and Prevention, FDA, and National Library of Medicine websites. Poison control centers: accredited by the AAPCC. Provides info for the public and health care providers. Most reliable and up to date sources of information.
General management
Supportive care
Evaluate and support vital functions as a first step until patient is stabilized. Airway, Breathing, Circulation (ABC).
History of exposure
Identity: of ingested substance, route of exposure, quantity ingested, time since ingestion, symptoms of overdose, associated illness / injury. Neurologic examination: seizures, altered consciousness, confusion, ataxia, slurred speech, tremor, headache, syncope. Cardiopulmonary examination: syncope, palpitations, cough, chest pain, shortness of breath, upper airway burning / irritation. GI examination: abdominal pain, nausea, vomiting, diarrhea, difficulty swallowing. Past medical history: Rx/OTC drugs, herbal medicines, alcohol / drug abuse, psychiatric history, allergies, occupational / hobby exposures, travel, domestic violence / neglect. Routine lab assessment: Complete blood count (CBC), serum electrolytes, BUN, serum creatinine, BG, urinalysis, ECG
Skin decontamination
Required when skin absorption may cause systemic toxicity or when contamination substance may produce toxic effects (e.g. acid burns). Remove clothes, irrigate area with plenty of water. DO NOT neutralized (exothermic reaction).
Gastric decontamination
Emesis Contraindications: children < 6 months, CNS depression, seizures, strong acids / alkali, sharp object, compromised airway, coma, convulsions, HC or petroleum distillates, patients already vomiting, substance that are very fast acting.
Syrup of ipecac: consider only if within 60 (even 30) minutes since ingestion, otherwise, no benefit. Onset of emesis: within 30 minutes, 3 vomiting episodes in 1 hour. SE: diarrhea, drowsiness, lethargy Gastric lavage Use: if patient is not alert or has gag reflex, if quantity was ingestion short while ago or if not responding to ipecac Procedure: aspire gastric contents instill 250 ml tap water or saline aspire repeat until content is clear for 2 liters. Activated charcoal Adsorbs the majority of substances. Always give ASAP. Exceptions: iron, lead, mercury, cyanide, lithium, ethanol, methanol, organic solvents, strong acids / alkali. Form: colloidal dispersion with water or sorbitol. Avoid multiple doses of cathartics may cause electrolyte imbalance, dehydration. SE: charcoal aspiration avoid if vomiting, bowel obstruction with multiple doses.
Dialysis
Last resort for decontamination. Hemodialysis or peritoneal dialysis. Hemodialysis: used for water soluble substances with Vd, MWt, protein binding. Use for life threatening ingestions of ethylene glycol, methanol. Can correct fluid and electrolyte abnormalities.
Hemoperfusion
Anticoagulated blood is passed through (perfused) a column containing activated charcoal or resin particles. Quicker than hemodialysis. Can NOT correct electrolyte / fluid abnormalities. Less effective for methanol / ethanol. SE: thrombocytopenia, leukopenia, hypoglycemia, hypocalcemia.
Alcohols
Ethylene glycol Forms: antifreeze, windshield deicing. Colorless, sweet taste.
Toxicokinetics: live metabolism by alcohol dehydrogenase glycoaldehydge by aldehyde dehydrogenase glycolic acid glyoxylic acid oxalic acid (most toxic). Symptoms: phase I (12 hr): tendon reflex, ataxia, nystagmus, metabolic acidosis, hypoca lcemia, phase II (1 day): tachypnea, cyanosis, tachycardia, pulmonary edema, phase III (2 days): flank pain, oligouric renal failure. Treatment: gastric lavage (within 30 min), IV ethanol, fomepizole (alcohol dehyrogenase inhibitor), pyridoxine / thiamine (convert glyoxylic acid to non-oxalate metabolites), sodium bicarbonate (correct acidosis), hemodialysis. Methanol Forms: gas-line antifreeze, windshield washe. Toxicokinetics: alcohol dehyrogenase formaldehyde formic acid. Symptoms: phase I: euphoria, muscle weakness, phase II: vomiting, diarrhea, dizziness, headache, dyspnea, blurred vision, photophobia, blindness, cardiac / respiratory depression, metabolic acidosis, hyperglycemia, coma, seizures, death. Treatment: gastric lavage (NOT charcoal), IV ethanol, fomepizole (alcohol dehydrogenase inhibitor), folic acid ( metabolism of format), sodium bicarbonate (correct acidosis), hemodialysis.
Antidepressants
Tricyclic antidpressants Toxicokinetics: t1/2 = 24 hr, liver metabolism, enterohepatic circulation, plasma protein binding. Symptoms: atropine-like SE (mydriasis, urinary retention, fever), tachycardia, BP, pulmonary edema, agitation, confusion, hallucinations, seizures. Lab data: ECG Treatment: GI decontamination (activated charcoal), alkalinzation (sodium bicarbonate to arterial blood pH), phenytoin / BZD (to control seizures, fosphenytoin cause less hypotension than phenytoin) , physostigmine (for anticholinergic symptoms, may cause asystole (no heart beat)) . Selective serotonin reuptake inhibitors (SSRI) Toxicokinetics: t1/2 = 24 hr, liver metabolism Symptoms: agitation, drowsiness, confusion, seizures Lab data: ECG Treatment: gastric lavage, supportive treatment
Anticoagulants
Heparin Dosage forms: IV, SC Toxicokinetics: t1/2 = 1 hr, liver metabolism Symptoms: bleeding, bruising Lab data: PTT, bleeding time, platelet count Treatment: Protamine IV (combines with and neutralized heparin), 1 mg protamine neutralized 100 heparin units. Warfarin Dosage forms: oral, parenteral Toxicokinetics: absorbed orally, t1/2 = 36 hr, 99% protein bound, 5-day activity duration. Symptoms: bleeding, bruising, hematuria, conjunctiva hemorrhage, GI / intracranial bleeding. Lab data: PT, INR, bleeding time. Treatment: Phytonadione (vitamin K), blood products with clotting factors.
BZD
Toxicokinetics: liver metabolism Symptoms: drowsiness, confusion, ataxia
Treatment: supportive (gastric emptying, activated charcoal, cathartic), Flumazenil (IV, short t1/2, careful observation for re-sedation in case of long acting BZD).
Beta blockers
Symptoms: hypotension, bradycardia, atrioventricular block, bronchospasm, hypoglycemia. Treatment: gastric lavage, activated charcoal, Glucagon, Epinephrine (.
Cocaine
Forms: alkaloid from Erythroxylon coca Toxicokinetics: good absorption from oral, inhalation, intranasal, IV route. Metabolized in the liver, excreted in the urine. Symptoms: CNS / sympathetic stimulation (BP, tachycardia, seizures, tachypnea). Death due to respiratory failure, cardiac arrest, MI. Treatment: Symptomatic. BZD for seizures. Labetolol for hypertension. Neuroleptics for psychosis.
Corrosives
Symptoms: strong acids and alkali cause skin burns. Treatment: decontamination. Irrigate exposed skin with water. AVOID neutralization (exothermic reactions more burns and tissue damage).
Cyanide
Forms: industrial chemicals, nail polish removers. Toxicokinetics: quick absorbed orally or by inhalation. Symptoms: headache, dyspnea, ataxia, coma, seizures, death Treatment: amyl or sodium nitrite converts hemoglobin to methemoglobin binds to cyanide ion (cyano-methemoglobin) sodium thiosulfate to regenerate hemoglobin. Oxygen for dyspnea. Sodium bicarbonate for acidosis.
Digoxin
Symptoms: confusion, anorexia, GI upset, dysrhythmia. Lab data: digoxin Cp, serum potassium, ECG. Treatment: ipecac or activated charcoal, correct blood potassium, heart support, Digoxin specific fab antibodies.
Electrolytes
Magnesium Forms: cathartics (mg citrate) mg with charcoal. Toxicokinetics: Mg is found intracellularly kidney elimination Symptoms: Mild weakness, tendon reflexes. Severe respiratory paralysis, heart block, ECG abnormalities. Treatment: 10% calcium chloride to temporarily antagonize cardiac effects of Mg. Use hemodialysis in severe cases. Potassium Toxicokinetics: main intracellular cation. acid-base balance potassium. pH potassium. Symptoms: cardiac irritability, peripheral weakness, bradycardia, dysrhythmia, ECG abnormalities.
Treatment: Calcium: antagonize cardiac effects of potassium , sodium bicarbonate: serum pH move potassium from extracellular to intracellular space, Glucose + insulin: move potassium from extracellular to intracellular space, cation exchange resins (sodium polystyrene sulfonate): bind potassium in exchange for sodium , hemodialysis: last resort for life-threatening hyperkalemia.
Iron
Elemental iron: 33% in fumarate, 20% in sulfate, 12% in gluconate. Symptoms: phase I (nausea, vomiting, diarrhea, GI bleeding), phase II (improvement within 24 hr), phase III (metabolic acidosis, renal / hepatic failure, pulmonary edema, death). Lab data: serum iron, hemoglobin, hematocrit, radiopaque pills in radiography. Treatment: Deferoxime chelates iron (red urine). Ipecac emesis if within minutes of small quantity ingestion. Whole bowel irrigation for quantities. Also, supportive treatment.
Isoniazid
Symptoms: nausea, vomiting, slow speech, ataxia, seizures, coma Lab data: lactic acidosis, hypoglycemia, hyperkalemia, leukocytosis Treatment: AVOID emesis (due to risk of seizures), quantity activated charcoal gastric lavage. Pyridoxine reverses isoniazid induced seizures (infusion in D5W). Sodium bicarbonate for acidosis.
Lead
Forms: paint or gasoline fume inhalation. Toxicokinetics: slow distribution (t1/2; 2 months). Symptoms: nausea, vomiting, GI pain, peripheral neuropathy, convulsions, coma. Lab data: anemia, lead level in blood. Treatment: Calcium EDTA (IM/IV), dimercaprol (IM).
Lithium
Toxicokinetics: absorbed orally, not plasma protein bound, small Vd, kidney eliminatin. Symptoms: Mild polyuria, blurred vision, tremor, weakness. Severe seizures, coma, delirium, fever. Lab data: determine the degree of toxicity form lithium Cp. Treatment: sodium polystyrene sulfonate, ipecac (within minutes), whole bowel irrigation (if quantity), hemodialysis (if acute exposure + severe symptoms).
Opiates
Toxicokinetics: methadone / heroin t1/2 Symptoms: respiratory depression, miosis, consiousness, hypotension, bradycardia. opiates are downers. Treatment: Naloxone (short t1/2, repeated dosing), Nalmefene (longer t1/2).
Organophosphates
Forms: pesticides, chemical warfare agents Toxicokinetics: absorbed through lungs, skin, GI, conjunctiva Symptoms: DUMBELSS: diarrhea, urination, miosis, bronchoconstriction, excitation, lacrimation, salivation, sweating. Lab data: RBC acetylcholinesterase activity. Treatment: atropine, pralidoxime (both IV).
Salicylates
Toxicokinetics: longer t1/2 at toxic doses Symptoms: Mild nausea, vomiting, tinnitus, malaise. Severe metabolic acidosis, convulsions, coma. Other SE: BI bleeding, PT. Treatment: ipecac emesis (if within minutes), doses repeated activated charcoal + a cathartic dose, moderate doses whole bowel irrigation, doses hemodialysis. Alkaline diuresis: with sodium bicarbonate to excretion. Fluid / electrolyte replacement. Vitamin K to correct coagulation.
Theophylline
Toxicokinetics: liver metabolism highly variable (depends on age, other drugs, disease). Symptoms: nausea, vomiting, seizures, dysrhythmias. Acute toxicity hyperglycemia, hypokalemia. SE are due to cAMP. Treatment: supportive (maintain airways, treat seizures, beta blocker (esmolol) treats tachycardia, disrhythmia), ipecac (if within minutes), repeated activated charcoal, whole bowel irrigation (if quantitiy), charcoal hemoperfusion or hemodialysis. Dosage forms: Toxicokinetics: Symptoms: Lab data: Treatment:
Registration requirements
All handlers of controlled drugs have to register with the DEA. DEA issues an Order To Show Cause to allow the registrant to appeal. Entities that must register: wholesalers (annual renewals), dispensers (pharmacies, practitioners) (renew every 3 years). Pharmacists / pharmacy employees do not have to register. Registration for separate activities: certain activies require separate registartion. Examples: manufacturing, distributing, dispensing, conducting research, narcotic treatment programs, chemical analysis, importing / exporting, maintenance, disposal, detoxification, packaging. Registration for separate locations: separate registration for each pharmacy, pharmacy chain, clinic, hospital. Wholesalers do not have to register if distributing to a registered location. Registration procedure: submit application to DEA by individual, partners, corporate officer, or person with power of attorney. Registration action by the DEA: certificate of registration is granted by DEA if appropriate, otherwise it may be denied. Modification of registration: e.g. for change of name, address, extension of authorized activities, etc. Transfer of registration: not allowed except in case of pharmacy ownership transfer.
Suspension / revocation of registration: May be due to imminent danger to public health or safety. If revoced / suspended deliver certificate or registration, any DEA 222 order forms, all controlled substances or place them under seal. Exemptions from registration: Military officials: can prescribe, administer or dispense but not purchase. Law-enforcement officials: federal and state. Civil defense officials: and disaster relief organizations during prolaimed emergencies or disasters. Agents and employees of registrants: such as pharmacists or delivery personnel. Termination of registration: death, cease of legal existence (company), d/c business or pratice. DEA must be notified and drugs disposed of.
Required inventories
All registered entities have to conduct biennial inventory. Initial inventory: must be taken on the day of start or end of business or change of ownership. If no controlled substances in possession must be documented. Biennial inventory: any date within 2 years of the previous inventory. Inventory procedures: conducted at either the open or close of business. Separate inventory record required for CII keep drugs separate. Inventory content: inventory must contain: date of inventory, dosage form, strength, number of units or volume in each container. Exact count is required for open bottle of only CII (estimate is OK for other Cs, unless containers contains > 1000 tablets). Inventory record maintenance: keep inventory separate at the registered location for 2 years. Keep CII inventory separate. Must be readily retrievable. Submission to DEA is not required. Perpetual inventories: not required. New or changes in schedules: inventory required for that drug only.
specialty if physician is a specialist. Can not prescibe controlled drugs for the sole purpose of detoxification of maintenance of addiction (only if within a treatment program). Prescribing CII must be written unless it is an emergency oral drugs only, no alternative, written Rx is not possible, only necessary quantity, prescriber must be known to the pharmacist written Rx must be provided within 7 days of oral Rx (mail or in person), otherwise notify DEA. CIII-V can be oral or fax. Faxed Rx: ok for CIII-V. For CII ok to prepare the Rx but no released to the patient without written Rx exceptions include injectable home health, hospice, and LTC Rx (no written Rx required) . Dispensing a Rx Time validity: 6 months for CIII-IV and no time limit for CII and CV (although questionable after 6 months). No limitation on quantity either. Apply good faith principles. All information on the Rx must be complete (including S/N). Label: must have pharmacy name / address, S/N, date of original filling, patient name, prescriber name, drug info, directions, Cautionary Auxilliary Sticker. Separate record files: CII, CIII-V, other Rx all separate, OR Combine all C with CIII-V or combine CIII-V with non control as long as C is stamped in red on CIII-V. Refills: no refills for CII. For CIII-IV up to 5 refills in 6 months. No limit for C-V (use good faith). Maintain either physical or computerized records (certain characteristics). Partial dispensing: allowed for CIII-IV within 6 months. For CII: allowed only if not enough stock(within 72 hr), or terminally ill patient / LTC (within 60 days). Transfer of refills (CIII-V): allowed only once. Write Void or Transfer on Rx.
Security considerations
Seals / seals: seals required for all packages and containers. Labels must clearly designate the schedule (II-V). Felony convictions: no registrant may employ felons conviced with a narcotic offense.
DEA inspections
Inspected are conducted only in a reasonable manner and during business hours, only after registrant notification or court warrant. Specifics of the inspection scope must be provided.
Phase 1: use on healthy humans to determien metabolism, pharmacology, mechanism, SE. Phase 2: well-controlled closely monitored studies on small # of patients to evaluate efficacy for a certain indication and also SE and risks. Phase 3: expanded clinical trials on patients to confirm safety and efficacy and risk/benefit relationship NDA: acceptable proof of safety and efficacy to the FDA approved for use in certain indications. Treatment INDs (treatment protocols): allows a practioner to use an investigational drug as treatmetn in serious and life-threatening disease when no alternatives are available.
OTC medications
FDA determined drug is safe for self-administration. Usually, drugs are not habit forming, toxicity / SE. Must have adequate clear directions and must comply with FDA monograph (to avoid misbranding). A legend (Rx) drug may be converted by the FDA to OTC.
Drug recall
Voluntary manufacturer recall: may be completely voluntary or after several attempts by the FDA to receive court ordered recalls. Drug recall classification: assigned by the FDA. Class I: potential for serious SE and death. Class II: potential for temporary or reversible SE or when serious SE are unlikely. Class III: not likely to cause serious SE Recall procedure: strategy should consider the depth of the recall, need for public warning. First layer of notification (to wholesalers) is done by the company. Public notification is made by the FDA in the weekly FDA Enforcement Report.
Package inserts
Manufacturers insert: full disclosure is required by the manufacturer. Enclosed with every commercial container. Contains essential informative and accurate scientific information for safe and effective drug use. It cant be promotional in tone, false or misleading. Patient package insert: due to certain SE with certain products, patient inserts must be dispensed, including refills. That includes the following: Oral contraceptives, IUDs, Estrogen products, Porgestational products, Isoproterenol inhalation products, Miscellaneous (e.g. Isotretinoin serious fetal harm if pregnant). For isoproterenol, label with Do not exceed prescribed dose. Contact physician if difficult persists.
Medical devices
Safety and effectiveness are required. Class I: reasonable assurance of safety and quality Class II: no reasonable assurance of safety and quality, but has sufficient info to establish controls to ensure safety and quality Class III: no reasonable assurance of safety and quality (generally, they can not be marketed). Medical device tracking: required if failure may lead to serious SE. Tracking allows recalls. Manufacturers reports: manufacturer, hospitals, pharmacies, etc are required to report to the FDA potential link to death or adverse SE. Misbranding and adulteration: same as drugs
Exceptions
Sublingual nitroglycerin and sublingual / chewable isosorbide dinitrate at low doses. Erythromycin ethylsuccinate granules for oral suspension ( doses). Oral contracpetives / conjugated estrogen / norethindrone acetate in memory-aid (mnemonic) packages ( dose). Medroxyprogesterone acetate tablets. Anhydrous cholestyramine powder. Colestipol powder ( dose) Potassium supplements (effervescent tablets, liquid, powder) ( dose). Sodium fluoride (tablet / liquid, dose). Betamethasone tablets in dispenser packages ( dose).
Prednisone or methylprednisolone tablets ( dose) Pancrelipase tablet, capsule, powder ( dose) Mebendazole tablets ( dose)
Anti-Tampering Act
Act passed in 1984 due to death from OTC capsules containing cyanide. Applies to consumer products (food, drug, device, cosmetic, other articles). OTC tamper-resistant packaging: required from some products (contact lens, ophthalmic solutions). Contain a visible indicator of breach or tampering. Product / tamper-resistant technology design must be distinct to avoid easy duplication by commonly available processes. OTC tamper-resistant labeling: clearly alert consumers to specific tamper-resistant feature on the package. Medical devices and cosmetics: required for certain products Violations: include tampering, false communication or conspiracy for either.
Information in medication order: patient information, date and time, name and dosage form, product strength, route of administration, signature, directions to the pharmacist, instructions for administration.
Understanding the Rx
Understanding the order: all info must be understood and consistent, including disease condition, reason for treatment, type of units used. Evaluating appropriateness: follow up if incomplete info was provided. Evaluate allergies, route of administration, drug-drug / food / disease interactions, safety for intended use, proper quantity and dosage, incompatibilities, legitimate prescriber. Discovering inappropriate Rx: Drug Utilization Review: review medication profiles to ensure appropriateness. Therapeutic intervention: calling the prescriber to discuss concerns regarding the Rx. Following the intervention, the Rx may be dispensed as written, with changes or not at all.
Processing the Rx
Involves use of technicians and automation, save pharmacists time for patient cou nseling and education. Record Rx number, original date of filling, product and quantity dispensed, pharmacists initials. Product selection: involves generic substitution, formulary / therapeutic substitution policies. Product preparation steps: obtain proper medication amount, reconstitute if necessary, extemporaneous compounding, assembly of delivery unit, selection of proper package or container. Labeling: contains name and address of pharmacy, patients name, original date of filling, Rx number, directions for use, product name and manufacturer, product strength, quantity dispensed, prescriber name, expiration date, pharmacist initials. Unit-dose packages: contain one dose or unit of medication, label identifies drug name, strength, lot#, expiration date. Auxiliary labels: to ensure proper medication use, storage, federal transfer of narcotics, etc. Record-keeping: include patient profile system that includes demographic information (allergies, DOB, disease, weight, occupation, OTC use) and record of all medications.
Patient monitoring
Pharmaceutical care plan: to frequency and benefits of desired outcomes. Includes: assessment (review medical conditions and symptoms), plan (decision on appropriate therapy), monitoring (review outcome goals and endpoints). Drug-related problems: unnecessary therapy, wrong drug, wrong dose, SE, poor compliance, need for additional therapy.
High-efficiency particulate air (HEPA) filters: used to clean the air entering the room. Remove all particulates < 0.3 mm with efficiency ~ 100%. HEPA filtered rooms are Federal Class 10,000, i.e., they contain <10,000 particles 0.5 mm or larger per cubic foot of air. Positive-air pressure flow: used to prevent contaminated air from entering a clean room. Counters: in the clean room are made of easily cleaned nonporous material, e.g., stainless steel. Wall / floors: free from cracks / crevices, rounded corners, made of nonporous material, easily disinfected. Air flow: air moved with uniform velocity (90 fpm) along parallel lines. Laminar flow hoods: clean air work benches in clean rooms designed as aseptic environment for making sterile products (Class 100). Horizontal: air flow moves across the surface of the work area (disadvantage: no protection for the operator). Vertical: advantages: air flows down on the work space, which protects the operator, portion of the air is circulated a second time. Inspection / certification: for clean rooms and laminar flow hoods is done annually or when moved. The dioctyl phthalate (DOP) smoke test ensures that no particle > 0.3 mm passes through HEPA filter. Anemometer is used to measure air flow velocity and a particle counter is used to count particles.
Packaging
Ampules: made entirely of glass. Single use. Disadvantages: glass fragments may contaminate the product during opening must be filtered, not multiple use. Not commonly used now. Vials: glass or plastic closed with a rubber stopper and sealed with aluminum crimp. Advantages: can be multiple use (if bacteriostatic agent is added), easier to remove product, no glass fragment risk, no need for filtration. Disadvantages: coring of rubber stopping can get into product, multiple use can cause microbial contamination. Drugs that are unstable in solution are packaged in solid form and must be reconstituted with a diluent (sterile water or NaCl) before use. Lyophilization (freeze drying) can be used to dissolution rate and permit rapid reconstitution. Double chamber system: one chamber with sterile water for injection is separated from unreconstituted drug chamber by rubber closure no need to enter vial twice contamination risk. Add-vantage system: drug is in a vial attacked to an IV bag for reconstitution. Add-vantage vial is screwed into the top of Add-vantage IV bag and rubber diaphragm is dislodged to allow the mixing. Prefilled syringed: for immediate drug administration in an emergency (epinephrine, atropine). Prefilled cartridges: ready to use parenteral packages with accuracy and sterility. Used for narcotics. Infusion solutions: Small Volume Parenterals (SVP): volume < 100ml. Large VP (LVP): volume > 100ml. Packaging materials: Glass: clarity for easy inspection, interaction with content. Plastic polymers: durability, easy storage / disposal, weight, safety, e.g., PVC and polyolefin.
Routes of administration
Subcutaneous: usually in the arm or thigh. Example: insulin.
Intramuscular: e.g. mid-deltoid, gluteus medicus, < 5ml. Used for prolonged or delayed absorption (e.g. methylprednisolone). Intravenous: most important and common, immediate therapeutic response, no recall of inadvertent overdose, e.g., antibiotics, cardiac drugs. Intradermal: only very limited volume, e.g., skin tests and vaccines. Intra-arterial: deliver drug concentration into target side with little dilution by circulation, e.g., diagnostic radiopaque materials and antineoplastics. Hypodermoclysis: injection of large volumes of solution into SC tissue to provide continuous abundant drug supply, e.g., antibiotics for children. Intraspinal: e.g. local anesthetics during surgery (lidocaine, bupivacaine). Intra-articular: injection into joint space, e.g., corticosteroids (hydrocortisone, methylprednisone) for arthritis. Intrathecal: injection into the spinal fluid, e.g., antibiotics, cancer chemotherapy.
Parenteral preparations
IV admixtures: one or more sterile drug product added to an IV fluid.
IV fluids
Used in preparation of parenteral products (vehicles for IV admixtures). Dextrose (d-glucose): 5% dextrose in water (D5W). Used for reconstitution, as hydrating solution. Higher concentration dextrose (e.g. D10W) provide source of carbohydrates in parenteral nutrition. pH of D5W is 3.5-6.5 instability of acid-labile drugs. Concentration > 15% give through central vein. Use cautiously in DM. Sodium chloride: usually as 0.9% solution isotonic (normal saline). NaCl 0.45% is half-normal saline. Used for admixtures, fluid and electrolyte replacement. Bacteriostatic NaCl for injection (0.9%): for multiple reconstitutions (bacteriostatic benzyl alcohol, propylparaben, methylparaben). Water: for reconstitution and dilution of NaCl, dextrose. Use Sterile or Bacteriostatic Water for Injection. Ringers solution: used post-surgically for fluid and electrolyte replacement. Lactated Ringers (Hartmanns solution): contains sodium lactate, NaCl, KCl, CaCl2, may be combined with D5W. Ringers injection: does not contain sodium lactate, may be combined with D5W.
IV electrolytes
Cations: Sodium: main extracellular cation, important for interstitial osmotic pressure, tissue hydration, acid-base balance, nerve-impulse transmission, muscle contraction. Examples: Na chloride, acetate, phosphate. Potassium: main intracellular cation, important for muscle (esp cardiac) contraction, neuromuscular excitability, protein synthesis, carbohydrate metabolism. Examples: potassium chloride, phosphate, acetate. Calcium: important for nerve impulse transmission, muscle contraction, cardiac function, bone formation, cell membrane permeability. Examples: calcium chloride, gluconate, gluceptate. Magnesium: important for enzyme activities, muscle excitability, neuromuscular transmission. Example: magnesium sulfate. Anions: Chloride: main extracellular anion. With sodium, it controls interstitial osmotic pressure, blood pH. Examples: sodium, potassium, calcium chloride. Phosphate: main intracellular anion. Important for enzyme activities, controlling calcium levels, buffer to prevent changes in acid-base balance. Examples: sodium, potassium phosphate. Acetate: bicarbonate precursor used as alkali to preserve plasma pH. Examples: sodium, potassium acetate.
Parenteral antibiotics
Route: direct IV, short term IV infusion, IM, intrathecal. Use: serious infections requiring concentration, GI is inaccessible.
Parenteral antineoplastics
May be toxic and hazardous during prep, administration. Safe handling: use vertical laminar flow hood, syringes and IV tubing with Luer-Lok fittings, closed-front cuffed surgical gowns, double layered gloves, negative pressure technique, final dosage adjustment with care, special care priming IV sets, prime before adding the drug, special disposal, wash hands, monitor health of personnel. Patient problems: Infusion phlebitis: vein inflammation, pain, swelling, heat sensation, site redness, avoid by drug dilution and filtration. Extravasation: infiltration of the drug into SC tissues surround the vein. Response: local hydrocortisone or anti-inflammatory, antidote with cold compress, warm compress to blood flow and wash vesicant away from damage tissue.
Irrigating solutions
Manufactured by the same standards for IV products but not intended for injection. Labeling differenced specified in USP, i.e., different acceptable particulate matter levels, volume, container design. Topical administration: packaged in pour bottles into desired. For irrigating wounds, moistening dressings, cleaning surgical instruments. Infusion: e.g., perfuse tissues to maintain integrity of surgical field, remove blood, clear field of view as in urologic surgeries. Add Neosporin G.U. irrigant, an antibiotic, to risk of infection. Dialysis (dialysates): e.g., in renal failure, poisoning, electrolyte disturbances. They remove waste matter, serum electrolytes, toxic products. Peritoneal dialysis: hypertonic dialysate (dextrose, electrolytes) is infused in the peritoneal cavity via surgically implanted catheter remove toxins by osmosis and diffusion finally drain. Antibiotics, heparin may be added. Hemodialysis: patients blood is transfused through a dialyzing membrane that removes toxins.
Syringes
Glass or plastic barrel and tight-fitting plunger, small opening to accommodate needle. Luer syringe: oldest, universal attachment for all needle sizes. Syringe volumes: 0.3 60 ml. Insulin syringes have unit graduations (100 units/ml) rather than volume graduations. Calibrations: may be metric or English, vary depending on size. Syringe tips: Luer-Lok: threaded to ensure needle fit tightly, for antineoplastic drugs. Luer-Slip: unthreaded so needle does not lock into place, may be dislodged. Eccentric: set off center to allow needle to remain to injection site and minimize venous irritation. Catheter: used for wound irrigation and enteral feedings and not for injections.
Intermittent infusion
Continuous drip infusion: slow infusion to maintain therapeutic level ro provide fluid and electrolyte replacement. Rate: ml/hr or drops/min. Use for drugs with narrow therapeutic index, e.g., heparin, aminophylline. Intermittent infusion: infusion at specific intervals (4hr), for antibiotics. Direct bolus injection: rapidly deliver small volume of undiluted drug. Use for immediate effect as in emergency. Additive set infusion: using volume control device, for intermittent delivery of small amounts. Piggyback method: used when drug cannot be mixed with primary solution, a supplemental secondary solution is infused through the primary system, avoids a second puncture or further dilution. Admixtures: also called manufacturers piggyback, a vehicle is added to the drug, example: Add-Vantage system. Intermittent infusion injection devices: also called scalp-vein, heparin-lock, butterfly infusion set. Permit intermittent delivery without multiple punctures or prolonged venous access. Use dilute heparin or normal saline to prevent clotting in the cannula.
IV incompatibilities
Types of incompatibilities Physical: mixing causes visible change in appearance. Example: evolution of CO2 when sodium bicarbonate and HCl are mixed. It can be a visible color change or pptn (e.g. phosphate and calcium). Chemical: chemical degradation causing toxicity or loss of activity. Complexation: such as calcium and tetracycline inactive tetracycline complex. Oxidation: when a drug loses electrons color change, inactivity. Reduction: when a drug gains electrons. Photolysis: chemical decomposition by light hydrolysis or oxidation color change. Therapeutic: e.g. bacteriostatic (tetracycline) then bactericidal (penicillin G) activity of penicillin G. Factors affecting compatibility pH: in pH incompatibility. Acid + base = salt may ppt.
Temperature: temp degradation. Use fridge or freezer. Degree of dilution: dilution ion interaction incompatibility. Length of time in solution: time chance of incompatibility Order or mixing: do not add incompatible drugs in sequence (e.g. calcium, phosphate), mix well. Preventing incompatibilities Administer solutions quickly after mixing, mix each drug well after addition, number of mixed drugs, consult references.
Process stimulation testing: duplicate sterile product production except that a growth media is used instead of drug product. Incubate final product: no growth successful aseptic technique. Documentation: of training procedures, QC results, laminar flow hood certification, production records, etc.
Elimination Kidney is the major route of elimination for water soluble drugs and metabolites. Processes involved: glomerular filtration, tubular secretion, tubular reabsorption. Filtration and secretion eilimination, reabsorption elimination. All processes are in neonates. Renal blood flow (important for glomerular filtration) is in neonates. Only unbound drugs undergo glomerular filtration.
Pregnancy
Fetal development
Withdraw all unnecessary medications 3-6 months before plans for conception. Blastogenesis: first 2-3 weeks after fertilization. Germ formation occurs. Embryonic cells are undifferentiated. Organogenesis: 2-8 weeks. Most critical period of development as organs start to develop. Drug exposure may cause major congenital malformations. Fetal period: 9 weeks to birth. At 9 weeks, the embryo is called a fetus. Maturation and growth occurs. Low risk of major congenital malformations.
instead), Drug withdrawal (habitual maternal use of barbiturates, narcotics, benzodiazepines, alcohol), Reduced birth weight (cigarette smokers, alcoholics, drug abusers), constriction of ductus arteriosus rd (NSAIDs in 3 trimester may cause pulmonary hypertension).
Geriatrics
People >65 years use 33-50% of all prescriptions. 75% of elderly are Rx users. 20% of elderly experience SE. Incidence of SE is 2-3x higher in elderly. SE may be overlooked in elderly because they are similar to disease symptoms. Causes of SE in elderly: polypharmacy, multiple diseases, more severe diseases, drug elimination, sensitivity to drug effects. One third of elderly use => 6 drugs. Polypharmacy drug interactions, drug-disease interactions. noncompliance in elderly especially with females, socioeconomic status, living alone, polypharmacy, multiple disease, complicated regimens. Disease noncompliance. Example: macular degeneration, cataract, hearing loss, arthritis, Alzheimer. Clinical trials during drug development may not test drugs on the elderly ( SE). osteoporosis fractures due to falls because of drugs causing dizziness, drowsiness, syncope, hypotension, blurred vision. Avoid long acting BZD, use lorazepam, oxazepam (no active metabolites, phase I metabolism).
Pharmacokinetics
liver metabolism (phase I) drug accumulation Absorption: can be affected by delayed gastric emptying, gastric pH, GI motility. Usually, the rate but not the extent of absorption is affected. Distribution: body fat / lean muscle mass ratio Vd of fat soluble drugs (diazepam, propranolol). total body water Vd of water soluble drugs (acetaminophen). serum albumin protein binding free drug (warfarin, phenytoin). Kidney excretion: very important. glomerular filtration, tubular secretion rate. 50% in renal function by age 70 in normal patients. Serum creatinine is not a good measure of renal function as creatinine also with age. dose of renally eliminated drugs to avoid SE and toxicity. Examples: digoxin, procainamide, H2 antagonists, lithium, aminoglycosides. Liver metabolism: phase I (but not phase II) metabolism and blood flow t1/2, SE of BZD, some analgesics.
Pharmacodynamics
Altered response to certain drugs. response to beta blockers, response to analgesics, BZD, warfarin. Generally, start low and titrate slow. sensitivity to anticholinergic SE avoid if possible.
Hematological tests
RBCs
RBC count: number of RBCs per cubic mm of blood, an estimate of the blood Hb content. Normal: 4.5 million/mm3 (higher in men). Hematocrit or packed cell volume (PCV): measures percentage (fraction) by volume of packed RBCs in whole blood after centrifugation. Hct is usually 3x the Hb value. Normal: 45% (higher in men). Low Hct anemia, over hydration, blood loss. High Hct polycythemia, dehydration. Hemoglobin test: measures grams of Hb in 100 ml (1dl) of whole blood, an estimate of the oxygen carrying capacity of RBCs. It depends on the number of RBCs and amount of Hb in each RBC. Normal: 15 g/dl (higher in men). Low Hb anemia. RBC (Wintrobe) indices: info on the size, Hb concent, Hb weight of RBCs. Used to categorize anemias. Poikilocytosis: in RBC shape as in sickle-cell anemia. Anisocytosis: in RBC size as in folic acid and iron deficiency anemia. Mean corpuscular volume (MCV): ratio of Hct to RBC count. Measures average RBC size (anisocytosis). Normal: 90. Low MCV microcytic anemia (iron deficiency). High MCV macrocytic anemia (folic acid or vitamin B12 deficiency). Mean cell Hb (MCH): measures amount of Hb in average RBC. Normal: 30. Mean cell Hb concentration (MCHC): measures average Hb concentration in average RBC. Normal: 35. Low MCHC hypochroma (pale RBCs) as in iron deficiency. RBC distribution width (RDW): normal RBCs are equal in size bell-shape normal histogram distribution high RDW in anemia (iron, folic acid, vitamin B12 deficiency). RDW is never below normal. Reticulocyte count: measures immature RBCs (reticulocytes), which contain nuclear material (reticulum), Normal: 1% of all RBCs. It measures bone marrow production of mature RBCs. High reticulocyte count hemolytic anemia, acute blood loss, response to treatment of factor deficiency anemia. Low reticulocyte count drug-induced aplastic anemia. Erythrocyte sedimentation rate (ESR): measures rate of RBC sedimentation of whole uncoagulated blood. It reflects plasma composition. Normal: 10 mm/hr (higher in females). High ESR acute or chronic infection, tissue necrosis, infarction, malignancy. Use to follow disease course, differentiate diagnosis (angina normal ESR, MI ESR).
WBCS
WBC count: number of WBCs in whole blood. Normal: 7000 / mm3. High WBC count (leukocytosis) due to infection (esp. bacterial), leukemia, tissue necrosis. Low WBC count (leukocytopenia) due to bone marrow depression due to cancer, lymphoma or antineoplastic drugs. WBC differential: evaluates the distribution and morphology of WBC cell types including granulocytes (neutrophils, basophils, eosinophils), and non-granulocytes (lymphocytes, monocytes).
Neutrophils: may be mature or immature. Chemotaxis: congregation of neutrophils at site of tissue damage of foreign body invasion first line defense phagocytosis and degradation of invaders. Neutrophilic leukocytosis (#, fraction of immature cells) systemic bacterial infection (e.g. pneumonia), viral infection, fungi, stress (physical, emotional, blood loss), inflammatory disease (rheumatism), drug hypersensitivity, tissue necrosis, leukemia, certain drugs (Ep, lithium). Neutropenia (#, < 1000/mm3) overwhelming infection as bone marrow is unable to keep up with demand, viral infections, chemotherapy drug reactions. Basophils: called mast cells in the tissues. Basophilia (#) leukemia. Eosinophils: associated with immune reactions. Eosinophilia (#) acute allergic reaction (asthma, hay fever, allergy), parasitic infections. Lymphocytes: critical for immunologic activity, produce antibodies. Types: T and B. Lymphocytosis (#) viral infection. Lymphocytopenia (#) severe debilitating disease, immunodeficiency, AIDS. Atypical lymphocytes in infectious mononucleosis. Monocytes: phagocytic cells. Monocytosis (#) TB, bacterial endocarditis, during recovery of acute infections.
Platelets (thrombocytes)
Smallest in size. Involved in clotting. Normal: 225,000 / mm3. Thrombocytopenia: platelets due to disease or drugs. Moderate: < 100,000. Severe: < 50,000.
Cardiac troponins
Use: identify MI injury, prognosis of unstable angina. More specific than CK-MB. Troponin T in cardiac and skeletal muscles. Tropnonin I only in cardiac muscle. Normal: Troponin T < 0.1 ng/ml, Toponin I < 1.5 ng/ml.
Serum bilirubin
Bilirubin is a breakdown product of hemoglobin, main bile pigment. Indirect bilirubin (unconjugated): bilirubin released from Hb breakdown, bound to albumin, water insoluble, not filtered by glomerulus. Direct bilirubin (conjugated): Unconjugated bilirubin travel to the liver separate from albumin conjugate actively secret to the bile filtered by the glomerulus. Normal values: Total bilirubin 0.5 mg/dl. Direct bilirubin 0.1 mg/dl. bilirubin tissue deposition jaundice. Causes: hemolysis, biliary obstruction, liver cell necrosis. Hemolysis: total but not direct bilirubin. Normal urine. Biliary obstruction: may be intra-hepatic (e.g. due to chlorpromazine), or extra-hepatic (biliary stone) total and direct bilirubin. Bilirubin present in urine dark color. Liver cell necrosis: due to viral hepatitis total and direct bilirubin. Bilirubin present in urine dark color.
Serum proteins
Normal total serum protein level: 7 g/dL. Transport agents. Albumin: made in the liver (liver disease albumin ) Globulin: albumin compensatory in globulin.
Urinalysis
Appearance
Normal urine: clear, pale yellow to deep gold color. Red urine: presence of blood or phenolphthalein (laxative) Brownish-yellow urine: presence of conjugated bilirubin.
pH
Normal urine: slightly acidic (pH = 6) Alkaline pH: due to acetazolamide use (bicaronaturia), or due to leaving urine sample at room temperature.
Specific gravity
Normal urine: 1.015 specific gravity: DM, glucose in urine, nephrosis (protein in urin) specific gravity: due to diabetes insipidus ( urine concentration).
Protein
Normal: 65 mg/24 hr. Glomerular membrane prevents most blood protein from entering urine Albuminurea: abnormal glomerular permeability. Proteinuria: due to kidney disease, bladder infection, fever
Glucose
Normal renal threshold for glucose: blood glucose of 180 mg/dl. Glycosuria: due to DM.
Ketones
Usually absent in urine. Excreted when body has used available glucose stores and began to metabolize fat due to uncontrolled DM, or due to carbohydrate diet Ketonuria. Ketone bodies: betahydroxybutyric acid (major), acetoacetic acid, acetone.
Microscopy
Hematuria: presence of RBCs may indicate trauma, tumor, systemic bleeding. Squamous cells indicated vaginal contamination due to menstruation in women. Casts: protein conglomerations may be due to renal disease. Crystals: pH-dependent, uric acid crystals in acidic urine, phosphate crystals in alkaline urine. Bacteria: usually absent in urine (sterile), if present may be due to UTI or urethral contamination.
Serum creatinine
Creatinine: metabolic breakdown product of muscle creatine phosphate Normal level: 1 mg/dl, but varies based on the muscle mass Creatinine excretion: by glomerular filtration and tubular secretion. serum creatinine renal insufficiency. 50% in GFR doubling of serum creatinine.
Creatinine clearance
Rate at which creatinine is removed from blood by the kidney. Normal value: 100 ml/min (100 ml of blood cleared of creatinine / min). Creatinine clearance parallels GFR, more sensitive than BUN. Creatinine clearance = (urine creatinine concentration x urine rate) / serum creatinine. Cockroft and Gault equation: used to estimate Clcr based on body weight, age, gender, and serum Cr when urine information is N/A.
Electrolytes
Sodium
Major extracellular cation. Cellular osmosis and water balance: controlled by sodium, potassium, chloride and water. Normal level: 140 mEq/L. Concentration is a ratio of Na to water. Na water balance not electrolyte balance. Na control: by antidiuretic hormone (ADH) and aldosterone. Hypothalamus release ADH from pituitary gland renal reabsorption of sodium.
blood Na, blood K, angiontesin II aldosterone (mineralocorticoid) release from adrenal cortex Na reabsorption in exchange for K urine secretion. Hyponatremia: due to Na loss (kidney disease), Na intake, overhydration (non -saline fluid replacement, water intake), mineralocorticoid ( Na reabsorption), SIADH. Hypernatremia: due to Na excretion, Na intake (hypertonic IV), dehydration (loss of free water as in diabetes insipidus), mineralocorticoid, Na drug (Na bicarbonate, ticarcillin).
Potassium
Most common intracellular cation. Normal level: 140 mEq/L intracellular, 4.5 mEq/L in blood (10% extracellular can not use that measure). Role: electrical conduction in heart and skeletal muscles, water balance, acid-base balance. K regulation: by kidneys, aldosterone, blood pH, insulin, K intake. blood pH blood potassium / blood sodium Hypokalemia: most K is lost through kidneys, due to vomiting, diarrhea, laxative abuse, diuretics (mannitol, thiazides, loop), mineralocorticoids, glucosuria, K intake, metabolic alkalosis / insulin / glucose (all move K intacellularly). Signs: fatigue, dizziness, ECG, pain, confusion Hyperkalemia: due to kidney elimination, K intake, cellular breakdown (tissue damage, hemolysis, burns, infections), metabolic acidosis, potassium sparing diuretics, ACE inhibitors.
Chloride
Major extracellular anion critical for acid-base balance. Not important clinically. Only confirms Na levels. Normal: 100 mEq/L Cl retention usually happens with Na and water retention. Anion gap = sodium (chloride + bicarbonate) Hypochloremia: due to fasting, diarrhea, vomiting, diuretics. Hyperchloremia: usually due to metabolic acidosis, or dehydrat ion, Cl intake, renal failure.
Bicarbonate / CO2
HCO3-/CO2 is the most important buffering system to maintain pH (acid base balance). Normal level: 25 mEq/L. Bicarbonate binds to hydrogen to form carbonic acid which can convert to CO2 and water. Hypobicarbonatemia: due to metabolic acidosis, renal failure, hyperventilation, diarrhea, carbonic anhydrase inhibitors, drug toxicity (salicylate, methanol, ethylene glycol). Hyperbicarbonatemia: due to metabolic alkalosis, hypoventilation, bicarbonate intak e, diuretics.
Minerals
Calcium
Role: bone integrity, nerve impulse transmission, muscle contraction, pancreatic insulin release, gastric hydrogen ion release, blood coagulation. Normal level: 10 mg/dl. Ca reservoir in bones (44% calcium) maintains plasma level. 40% of calcium is bound to plasma proteins (albumin) Only free unbound ionic calcium is important physiologically depends on amount of serum protein (albumin) Hypocalcemia: due to parathyroid hormone or vitamin D. Can be caused by loop diuretics. Hypercalcemia: due to malignancy or metastasis, hyperparathyroidism, Pagets disease, thiazide diuretics, Ca intake, vitamin D.
Phosphate
PO4 is a major intracellular anion source of phosphate for ATP and phospholipids synthesis. Normal level: 4 mg/dl. Ca and PO4 are affected by same factors consider together Hypophosphatemia: due to vitamin D, hyperparathyroidism, malnutrition / anabolism, aluminum antacids, Ca acetate, alcoholism Hyperphosphatemia: renal insufficiency, vitamin D, parathyroid
Magnesium
Second most abundant intracellular and extracellular cation. Role: activates enzymes for carbohydrate / fat / electrolyte metabolism, protein synthesis, nerve conduction, muscle contraction. Normal level: 2 mEq/L. Hypomagnesemia: more common, due to GI absorption, GI fluid loss, renal loss. Signs: weakness, tremor, reflexes, arrhythmia. Hypermagnesemia: due to Mg intake with renal insufficiency, Addisons disease. Signs: bradycardia, flushing, sweating, Ca.
Summary table Indicator RBC Count Hematocrit (packed cell volume) Hemoglobin Poikilocytosis Anisocytosis Mean corpuscular volume Normal 4.5 million/mm3 45% (~3xHb) 15 g/dl RBC shape RBC size 90: average RBC size, (Hct / RBC count) 35: average Hb / RBC Normal distribution 1% immature RBCs (reticulocytes) 10 mm/hr High in men, erythropoiesis as in hypoxemia in men, polycythemia, dehydration in men sickle-cell anemia folic acid, iron deficiency anemia folic acid or vitamin B12 deficiency anemia Low
anemia (iron, folic acid, vitamin B12 deficiency) hemolytic anemia, acute blood loss in females, acute or chronic infection, rheumatoid arthritis, tissue necrosis, MI, malignancy Leukocytosis infection (esp. bacterial), leukemia, tissue necrosis Neutrophilic leukocytosis bacteria (pneumonia), viral, fungi, stress, rheumatism, drug hypersensitivity, tissue necrosis, leukemia Lymphocytosis viral infection Basophilia leukemia Monocytosis TB, bacterial endocarditis Eosinophilia acute allergy, parasite infection
WBC Count
7000 / mm3
Neutrophils
Leukocytopenia bone marrow depression due to cancer, lymphoma, antineoplastic drugs Neutropenia (<1000/mm3) overwhelming infection, chemothrepay Lymphocytopenia severe debilitating disease, immunity, AIDS
Lymphocytes
Platelet Count
225,000 / mm3
Thrombocytopenia (disease or drugs) acute MI (necrosis), skeletal muscle damage MI injury, prognosis of unstable angina MI, liver / lung disease biliary obstruction (bile duct stone), Pagets disease, osteomalacia, hyperparathyroidism Acute hepatitis, cirrhosis, fatty liver, liver congestion (as in CHF). severe liver damage, less sensitive / more specific than
Creatinine kinase (CK) Cardiac troponins Troponin-T<0.1 Toponin I < 1.5 ng/ml.
Asparatate aminotransferase (AST) (also celld SGOT) Alanine aminotransferase (ALT) (also called SGPT)
AST Liver enzymes Total serum bilirubin (Indirect / unconjugated + direct conjugated) Direct serum bilirubin (conjugated) Serum proteins LDH/ALP/AST/ ALT (above) 0.5 mg/dl liver dysfunction / damage Jaundice (hemolysis, biliary obstruction, liver cell necrosis) biliary obstruction, liver cell necrosis ( bilirubin may also show in urine dark urine) (not bound to albumin, secreted to bile, filtered) Liver disease, nephritic syndrome, cystic fibrosis) Brownish-yellow conjugated bilirubin Acidic: vitamin C, ammonium chloride diabetes insipidus ( urine concentration).
Urine color pH Specific gravity Protein Glucose Ketones RBCs Squamous cells Casts Crystals Bacteria Blood urea nitrogen Serum creatinine Creatinine clearance Sodium
Clear yellow to deep gold Slightly acidic (6) 1.015 65 mg/24 hr. 180 mg/dl None None None None None None 13 mg/dl. (60% of GRF) 1 mg/dl 100 ml/min 140 mEq/L
Red blood or phenolphthalein Alkaline: acetazolamide, bicaronaturia DM, glucose or protein (nephrosis) in urine glomerular permeability, infection, disease Glycosuria: due to DM Ketonuria: uncontrolled DM trauma, tumor, systemic bleeding vaginal contamination due to menstruation protein conglomerations due to renal disease Acidic uric acid crystals Alkaline phosphate UTI, urethral contamination renal disease, renal bl. flow, protein intake renal insufficiency Na intake, hypertonic IV, dehydration, diabetes insipidus, Na drug (Na bicarb), mineralocorticoid). intake, cellular breakdown (hemolysis, burns, infections), metabolic acidosis, K sparing diuretics, ACE-I metabolic acidosis, intake, dehydration, renal failure metabolic alkalosis, hypoventilation, bicarbonate intake, diuretics. parathyroid, vitamin D, thiazides, Pagets disease, intake, malignancy, metastasis, vitamin D, parathyroid, renal insufficiency intake, renal insufficiency, Addisons disease.
liver disease (protein is broken to urea in liver) renal insufficiency kidney disease, dietary intake, water intake, overhydration, mineralocorticoid, SIADH K intake, vomiting, diarrhea, laxative abuse, diuretics, glucosuria, metabolic alkalosis, insulin / glucose, mineralocorticoids fasting, diarrhea, vomiting, diuretics metabolic acidosis, renal failure, hyperventilation, diarrhea, carbonic anhydrase inhibitors, salicylate, methanol parathyroid, vitamin D, loop diuretics vitamin D, parathyroid, malnutrition / anabolism, aluminum antacids, Ca acetate, alcoholism GI absorption, GI fluid loss, renal loss
Potassium
Chloride Bicarbonate
Calcium
10 mg/dl
Phosphate
4 mg/dl
Magnesium
2 mEq/L
Electrophysiology
Conduction system Two electrical sequences: 1. Impulse formation: occurs first as a result of automatic electrical impulse. 2. Impulse transmission: occurs second to signaling the heart to contract. SA node AV node Bundle of His Purkinje fibers Conduction system structures (see figure): tissues that can generate or conduct electrical impulses. Sinoatrial (SA) node: main heart pacemaker, in the wall of the right atrium, spontaneously start action potential triggering atrium contraction. Atrioventricular (AV) node: in the lower interatrial septum, delays impulse briefly to allow complete atrium contraction and ventricle filling before ventricle contraction. Bundle of His: muscle fibers from the AV junction, impulses travel along bundle branches. Purkinje fibers: network that ends in the ventricular surface ventricle contraction. Latent pacemakers: AV node, bundle of His and Purkinje fibers contain cells that can generate impulses but at slower firing rate (called Overdrive Suppression in case of SA node damage or depression). Myocardial action potential Depolarization and repolarization: caused due to Na / K exchange in electrical potential across cell membrane. Has to occur before cardiac contraction. Phase 0 (rapid depolarization): rapid sodium influx to cell, cell membrane electrical charge changes from ve to +ve. Phase 1 (early rapid repolarization): Na channels close, potassium leaves the cell return to resting potential. Phase 2 (plateau, absolute refractory period): more potassium out, also calcium enters the cell, cell cannot respond to any stimulus Phase 3 (final rapid ventricular repolarization): more potassium ions out complete repolarization membrane electrical charge back to ve. Called relative refractory period: phase 3, responds only to strong stimuli. Phase 4 (slow depolarization): back to resting state with potassium in and sodium and calcium out. Fast channels (sodium): in heart muscle cells rapid depolarization. Slow channels (calcium): electrical cells of SA node and AV junction slow depolarization. Electrocardiography (ECG) (PQRST) P wave: atrium depolarization (activation). PR inverval: impulse spreads from atria to Purkinje fibers. Delay by AV node to allow ventricle filling. by digitalis. QRS complex: ventricular depolarization. by mexiletine, quinidine, class IC ST segment: beginning of ventricular repolarization, phase 2 (absolute refractory period), in angina
T wave: ventricular repolarization (phase 3), inverted in angina QT: ventricular depolarization and repolarization. by quinidine, procainamide, sotalol,
Clinical evaluation
Physical findings Chest pain, brain perfusion anxiety / confusion, dyspnea, cyanosis, abnormal pulse rate / rhythm, palpitations, BP, syncope, weakness, convulsions, urine output. Diagnostic test results ECG: a 12-lead ECG provides definitive diagnosis. Electrophysiologic testing: intracardiac procedure that determines the location of ectopic center and the need for packemaker / surgery. Probes are hooked through veins and arteries each heart segment is stimulated until arrhythmia occurs. His bundle study: locates origin of heart block / re-entry pattern Laboratory findings: test for hyperkalemia or hypocalcemia.
Drugs
Class IA IB IC II III IV Other Action Sodium channel blockers, conduction Also prolong repolaziation (K blocker) Sodium channel blockers, condcution Sodium channel blockers, condcution Beta blockers Potassium channel blocker prolong action potential. Calcium (slow) channel blockers Drugs quinidine, procainamide, disopyramide lidocaine, phenytoin, tocainide, mexiletine flecainide, propafenone, moricizine propranolol, acebutolol, esmolol Bretylium, sotalol, amiodarone verapamil, diltiazem adeonsine, magnesium, atropine, digoxin
Mechanism: Na and also K channel blocker. SE: GI upset, diarrhea (use Al hydroxide), Narrow therapeutic index (target 3 ug/ml). Toxicity: conduction SA block. Cinchonism: tinnitus, hearing loss, blurred vision, photophobia, diplobia, psychosis. CI: AV block, prolonged QT interval (may cause torsades, quinidine syncope and sudden death). dose in liver dysfunction and elderly. DI: cause digitalis toxicity, severe BP with vasodilators, alkalinizers cause toxicity. Procainamide IV/IM (acute) and as SR orally (long term therapy). N-acetylprocainamide: active metabolite. SE: SLE (arthlagia, myalgia, fatigue), anticholinergic, GI upset than quinidine. Narrow therapeutic index (target 7 ug/ml). Toxicity: ventricular arrhythmia, conduction SA block. CI: procaine hypersensitivity, myasthenia gravis, prolonged QT interval, torsades, AV block, SLE. dose in CHF (due to Vd), in kidney or liver damage. Disopyramide SE: ventricular dysfucntion, anticholinergic (dry mouth, constipation, etc). Targel level: 3 ug/ml. Used orally CI: AV block, cardiogenic shock, CHF, myasthenia gravis. Class IB Lidocaine IV/IM. For arrhythmia due to MI and heart surgery SE: hemodynamic compromise, CNS (dizziness, resltessness, tremors, convulsions), tinnitus, blurred vision. Target: 4 ug/ml. Toxic metabolites (glycinexylidide). DI: toxicity with phenytoin and beta blockers. Phenytoin Orally or IV. To treat digitalis-induced arrhythmia (mostly), acute MI, heart surgery. SE: SLE, gingival hyperplasia, nystagmus, CNS (drowsiness, ataxia, vertigo), cardiac SE. Target: 14 ug/ml. Chronic use can cause toxicity. Multiple drug intractions toxicity. Hypersensitivity reactions: blood, skin, Stevens-Johnson, and liver. Tocainide Similar structure to lidocaine except taken orally (avoid in lidocaine hypersensitivity). SE: CNS (dizziness, restlessness, tremors, confusion), GI upset, diarrhea, blurred vision, blood. Target: 6 ug/ml. Mexiletine Similar structure to lidocaine but first-pass metabolism taken orally. SE: dizziness, ataxia, BP, QRS complex, blood, liver. Toxicity: tremor. Target: 1 ug/ml. Class IC Prolong QRS complex, slow of phase 0 (rapid depolarization) and slow conduction, no effect on repolarization. May mortality due to pro-arrhythmic effect use is questionable. Orally. Flecainide: Use only in refractory life-threatening ventricular arrhythmia. SE: -ve inotropic effect (CI in CHF), CNS (dizziness, headache, tremor), GI upset, blurred vision. Target: 1 ug/ml. Propafenone: SE: dizziness, headache, GI upset, bitter taste. Target: 0.5 ug/ml. Moricizine: SE: dizziness, headache, GI upset. IC: CV (arrhythmia esp in MI), eye toxicity (blurring, diplobia)
Mechanism: heart stimulation, AV impulse conduction, refractory period heart rate, heart oxygen demand. Propranolol: IV or oral for tachy-arrhythmia due to catecholamine stimulation, digitalis-induced ventricular arrhythmias. SE: BP, cardiac arrest ( AV conduction), fatigue, bronchospasm. Sudden d/c acute MI, arrhythmia, angina dose gradually. CI: AV block, cardiogenic shock, CHF, asthma, DB (masks hypoglycemia). Esmolol: very short t1/2 (minutes), give IV. SE: BP, dizziness, headache, fatigue, GI upset, bronchospasm. CI: CHF.
Unclassified antiarrhythmics
Adenosine Mechanism: naturally occurring nucleoside in all body cells. Acts on G-protein coupled adenosine receptors AV node refractoriness AV conduction, re-entry through AV node. Given IV (very short t1/2, 10 seconds). SE: short-lived flushing, BP, sweating, palpitations, short breath, chest pressure (bronchospasm, X theophylline). DI: antagonize methylxanthines (caffeine, theophylline) effect. Other uses: exercise tolerance during exercise testing. Atropine Use: IV for sympathetic sinus bradycardia. Mechanism: blocks vagal effects on SA node AV conduction heart rate. Initial doses may cause reflex bradycardia. Also Digoxin: vagotonic response of impulse generation AV node refractoriness.
Risk factors: hyperlipidemia (cholesterol > 200 mg/dl, LDL > 130 mg/dl, HDL < 35 mg/dl), hypertension, smoking, diabetes, obesity, family history, sedentary life style, chronic stress type A personality, age, male gender, oral contraceptives, gout. Factors that O2 demand: exercise, smoking, cold temp.
Etiology
1. blood flow: atherosclerosis with or without coronary thrombosis is the most common cause. Coronary arteries are progressively narrowed by smooth muscle cell proliferation and accumulation of lipid deposits (plaque). Coronary artery spasm is a sustained contraction that can occur spontaneously or induced by irritation (catheter, hemorrhage), cold exposure, ergot drugs. The spasm can cause Prinzmetal angina or MI. Traumatic injury such as impact of steering wheel on the chest. Embolic events can also occur abruptly. 2. blood oxygenation: blood oxygen carrying capacity in anemia. 3. oxygen demand: can occur with exertion or emotional stress (sympathetic stimulation). Systole: two phases (contraction and ejection). Contractile (inotropic) state affects oxygen requirement. ejection time oxygen demand.
Angina Pectoris
Episodic reversible oxygen insufficiency. May be caused oxygen imbalance (tachycardia, anemia, hyperthyroidism, hypotension, arterial hypoxemia). . Patient complaints: squeezing pressure, sharp pain, burning, aching, bursting, indigestion-like discomfort, radiating pain to the arms / legs / neck / shoulders / back. Physical examination: usually not revealing, especially between attacks. Note history, risk factors, description of attacks, precipitation patterns, intensity, duration, relieving factors. Treat risk factors: Hypertension should be controlled. Obesity should be through diet and exercise. Smoking should be stopped, but watch for anxiety. Quitting results in 50% in morality. Transdermal nicotine patches helps quitting over 10 weeks using decreasing doses of nicotine. Nicotine gum and bupropion can also be used. Also, clonidine. Types Stable (classic / exertion) angina: most common form, usually due to a fixed obstruction in a coronary artery. Triggered by exertion, emotional stress or heavy meal and relieved by rest or nitroglycerin. The pain builds a peak radiating to the jaw, neck, shoulder, arms and then subsides. Prinzmetals angina (vasospastic or variant angina): due to coronary artery spasm ( blood flow). Initially occurs at rest, pain may disrupt sleep. Calcium channel blockers are preferred over beta blockers. Nitroglycerin may not help. Unstable angina: due to significant coronary artery vasospasm and platelet aggregation. Characteristics: may occur at rest, response to nitroglycerin, pattern change / severity. Progressive unstable angina may signal imminent MI. Immediate hospitalization required. Nocturnal angina (angina decubitus): occurs in the recumbent position and is not related to rest or exertion. Occurs due to ventricular volume ( demand). Relieved by diuretics ( left ventricular volume). Nitrates may improve nocturnal dyspnea. Diagnostic tests ECG: normal in 60% of patients. May show Q-wave, T-wave inversion, ST segment. Stress / exercise ECG: helps diagnose patients with normal ECG. ST -segment. 201 99m Stress perfusion imaging: with thallium or technetium sestamibi. Expensive. Pharmacologic stress test: when coronary artery disease is suspected but patient cant exercise. Use IV dipyridamole, adenosine ( AV conduction), dobumatime to induce cardiac ischemia in ECG. Coronary arteriography / cardiac catheterization: very specific, sensitive, invasive, expensive, risky (2% mortality rate). Antihyperlipidemics Bile acid sequestrants: Cholestyramine chloride is a basic anion-exchange resin. Colestipol HCl is a copolymer. Mechanism: insoluble, nonabsorbable, hydrophilic, anion-exchange resins bind bile acids in the intestine bile acid synthesis from cholesterol cholesterol depletion. SE: bad taste (before
meals), GI upset, constipation, bloating, dyspepsia, other drugs absorption (e.g. digoxin, may use in toxicity), fat soluble vitamine (ADEK) deficiency. Statins: lovastatin, atrovastatin, simvastatin, pravastatin, fluvastatin, cerivastatin. Preferred in the evening. Mechanism: HMG-CoA reductase (converts HMG-CoA to mevalonate; precursor for cholesterol) cholesterol synthesis. SE: liver toxicity, monitor creatine kinase (CK) in case of skeletal muscle complaints (myopathy, rhabdomyolysis), headache, rash. CI: fibrates / cyclosporins risk of liver damage. Fibric acid derivatives: gemfibrozil, clofibrate, fenofibrate (micronized prodrug). Mechanism: synthesis / catabolism of triglycerides / cholesterol. SE: GI upset, liver function (monitor combined use for statins). Niacin (nicotinic acid): SE: flushing / itching (tolerance in 2 weeks, may be with aspirin), liver function, GI upset. Other drugs: probucol, eicosapentaenoic acid (EPA), docashexanoic acid (DHA). Probucol SE: arrhythmia, syncope. Nitrates Chemistry: Nitrites (amyl nitrite) organic esters of nitrous acid, Amyl nitrite: very volatile, flammable liquid, by inhalation for CN poisoning. Nitrates (nitroglycerin, isosorbide) organic esters of nitric acid. Nitroglycerin: very volatile, flammable, special storage, dispense in original glass container, protect from body heat, special IV plastic tubes to avoid absorption and loss of effect, extensive first pass effect (use transdermal or sublingual). Dosage form: Nitroglycerin: sublingual / buccal tabs, topical ointment, transdermal, IV. Isosorbide mono / dinitrate: tablets. Mechanism: fast acting, form free radical nitric oxide (NO, endothelium-derived relaxing factor, EDRF) activates guanylyl cyclase cGMP dephosphorylation of myosin light chain muscle relaxation, venous dilation ( vascular resistance) peripheral blood pooling venous return preload (left ventricular volume) respiratory symptoms (shortness of breath, nocturnal dyspnea). Also arterial pressure afterload oxygen demand. Also some in afterload. Use: use sublinigual (up to 3 tabs in 15 minutes), transmucosal (buccal tabs / spary) or IV nitroglycerin for acute attacks of angina pectoris. Sublingual tabs / oral tablets / transdermals can be used prophylactically before known stress (eating, sex). IV nitroglycerin is used for emergency unstable angina. SE: may BP reflex tachycardia / postural hypotension, headache (transient, temporary, prevented by Tylenol 15-30 beforehand), dizziness, methemoglobinemia Nitrate tolerance: loss of efficacy, avoid by requiring 12hr nitrate free periods. Otherwise, higher doses may be required. Beta blockers Mechanism: sympathetic heart stimulation (B1) heart rate and contractility (-ve inotropic / chronotropic) oxygen demand at rest / exertion, arterial blood pressure. Use: with nitrates to frequency and severity of exertional angina. May narrow coronary artery combine with calcium blocker, avoid in Pr inzmetals angina. Use propranolol. SE: bronchoconstriction, mask hypoglycemia (tachycardia), cardiac compensation (fatigue, shortness of breath, edema, dyspnea). Withdrawal syndrome and angina / MI if suddenly d/c. Calcium channel blockers Mechanism: prevent / reverse coronary spasm by calcium influx into smooth / cardiac muscle blood flow / oxygen supply. Also dilate arterioles and heart contractility total peripheral resistance oxygen demand / afterload. nd Use: 2 choice to nitrates and beta blockers in stable angina (may combine). Critical in Prinzmetals angina / angina at rest. Diltiazem / verapamil / bepridil: watch for heart block / cardiac compensation due to ve inotropic effect. Careful with other ve inotropic drugs (beta blockers, anti-arrhythmics). Verapamil constipation straining and oxygen demand. Nifedipine: peripheral vasodilation, limited ve inotropic effect. SE: hypotension, tachycardia (combine with beta blocker), dizziness, edema.
Other drugs: Maximal therapy: nitrate, CCB, beta blocker combination. Morphine: in unstable angina when nitroglycerin fails. Aspirin: use indefinitely in stable and unstable angina. Heparin/enoxaparin/dalteparin: with aspirin in unstable angina.
Myocardial infarction
Severe prolonged deprivation of oxygen to part of the heart irreversible necrosis. Usually due to occlusive thrombus near a ruptured atherosclerotic plaque. May lead to ventricular fibrillation (most disorganized arrhythmia) cardiac arrest and death (sudden death syndrome). Mortality rate: 30%. Signs and symptoms: Persistent severe chest pain or pressure (crushing, squeezing, elephant heavy). Pains beings in the chest and may radiate to the left arm, neck, leg, etc. Onset of pain is not associated with exertion. Unlike in angina, pain persists > 30 minutes and is not relieved by nitroglycerin. MI may be silent (no pain). Other symptoms: anxiety, impending doom, sweating, GI upset. Complications Lethal (ventricular) arrhythmia: arrhythmias resistant to lidocaine may respond to procainamide and bretylium. CHF: left ventricular failure pulmonary congestion diuretics. Digoxin contractility, compensate for heart damage. Cardiogenic shock: due to cardiac output. Occurs when area of infarction > 40% and compensatory mechanisms are ineffective. Vasopressors (alpha stimulants to BP) and inotropes may be used. Use vasodilators (nitropursside) to preload and afterload. Intra-aortic balloon pump may be used. Diagnostic tests Because MI is life threatening emergency, diagnosis is presumed and treatment is initiated based on complaints and immediate 12-lead ECG. Serial 12-lead ECG: abnormalities may be absent in the first few hours. ST elevation. Ventricular premature beats and ventricular arrhythmia are the most common arrhythmia. Cardiac enzymes: creatine kinase (MB-CK) is elevated within hours, peaks at 24 h and back to normal at 72 h. Cardiac troponin I and T (cTnI, cTnT) patterns are similar to MB-CK but more sensitive. 99m Lactase dehydrogenase (LDH) use is not longer common. Cardiac imaging include tc pyrophosphate scintigraphy, myocardial perfusion, radionucleotide ventriculography, coronary angiography. Treatment: Nitrates: may chest pain anxiety and catecholamine release ( coronary spasm less in oxygen demand). Morphine: causes venous pooling and preload, cardiac workload, oxygen consumption (IV). Drug of choice to MI pain and anxiety. SE: orthostatic hypotension, respiratory depression, constipation (use docusate). Vagomimetic effect bradyarrhythmia (if excessive reverse using atropine). Oxygen: Three liters/min via nasal cannula for chest pain, hypoxia and ischemia Warfarin: for treatment of acute MI to mortality, prevent recurrence, complications (stroke). Target INR: 2.5-3.5. Antiplatelet agents: abciximab, eptifibatide, tirofiban platelet glycoprotein receptors. Beta blockers: propranolol, metoprolol, atenolol. Given in early acute MI to oxygen demand, cardiac workload, ischemia, infarction post MI mortality. ACE inhibitors: after MI to exercise capacity, mortality in case of CHF, ventricular remodeling. Antihyperlipidemics: cholesterol MI mortality. Calcium channel blockers: avoid in acute MI or in left ventricular malfunction. incidence of reinfarction. Dipyridamole: relax smooth muscles, coronary vascular resistance (blood flow). Also, anti -platelet action. Used for angina pectoris prophylaxis. SE: BP, headache, dizziness. Others: intra-aortic balloon, coronary angiography, PTCA.
Thrombolytic agents Atherosclerotic plaques are made of lipids and fibrous proteins. Lesion rupture triggers release of serotonins, thromboxane A2 and adenosine diphosphate alteplase platelet aggregation clot. The resulting fibrin traps RBCs, platelets, plasma proteins to form thrombus. Clot dissolution is caused by conversion of plasminogen to plasmin mediated by plasmingoen activators. Use as early as possible (<12 h after pain starts). Absolute CI: internal / eye hemorrhage, intracranial / intraspinal injury, pregnancy, aneurysm, hypertension. Recombinant tissue plasminogen activator (t-PA): front-loaded regimen (IV bolus then infusion). Streptokinase (SK): SE: systemic antibody formation chances of refractory response and allergy if repeated within 6 months (avoid if unknown). Monitor for bleeding, reperfusion arrhythmia (within 30 min), hypotension, anaphylaxis. Other thrombolytic agents: reteplase, tenecteplase, anisoylated plasminogen streptokinase activator complex (APSAC). Post thrombolysis adjunctive therapy: antiplatelet and anticoagulant therapy after reperfusion to prevent reoccolusion, ischemia and reinfarcation. Aspirin: during thrombolyic therapy post-infarct mortality. Also: clopidrogel, ticlopidine, dipyridamole. Heparin: with thrombolytics to prevent reocclusion after reperfusion, mortality in MI. Give bolus then infusion. Goal: maintain APTT (activated partial thromboplastin time) at 1.5 20 times control. Avoid combining with streptokinase ( bleeding). Give SC, but not IM. Alternatives: MWt heparins (enoxaparin, dalteparin).
39. Hypertension
Pathophysiology
Arterial pressure = cardiac output X Peripheral Resistance Cardiac output = heart rate X stroke volume Conditions that increase stroke volume: fever, aortic regurgitation, thyrotoxicosis Starling's Law: ventricular stretch myocardial contractility blood volume returning ventricular dilation Initiators of baroreceptor reflexes: stretch receptors located in the wall of large chest and neck arteries Causes of hypertension: Cushing's disease, oral contraceptives, acromegaly, polycystic kidney disease Hypertension of unknown etiology: essential hypertension, toxemia of pregnancy, acute intermittent porphyria Essential hypertension: unknown cause (90% of cases). Chronic vasoconstriction ( tone). Endocrine hypertension: pheochromocytoma (tumor causing in catecholamine release) Renal hypertension: chronic pyelonephritis. Neurogenic hypertension: familial dysautonomia Other causes: aortic coarctation Factors causing systolic hypertension with wide pulse pressure: stroke volume. aortic compliance Anesthetized patients receiving antihypertensives responses to body position changes and acute blood loss, altered responses to sympathomimetic drugs Perioperatively: antihypertensive drug treatment should be maintained Rapid increases in BP vagal center excitation negative iontropic effect ( contractility), negative chronotropic effect ( heart rate). Africans: use Ca channel blockers and diuretic (CaD) (ACE inhibitors/beta blockers less effective) Generally, avoid prescribing two drugs from the same therapeutic class. Effectiveness of antihypertensive drugs is highly unpredictable, requires dose/drug adjustments. Withdrawal antihypertensives gradually to reduce SE (e.g. MI with b-blocker) Elderly: esp. vulnerable to CNS SE, orthostatic hypotension. Lower doses may be needed.
Diuretics
Use: recommended (with beta blockers) as initial therapy for BP. Diuretics are also used for CHF, edema, fluid retention. Precaution: take during the day to avoid interruption of sleep due to frequent urination. May raise lithium level (CI)
Thiazide diuretics
Examples: Chlorthiazide, hydrochlorthiazide, cyclothiazide, polythiazide, trichlormethiazide, methyclothiazide, hydroflumethiazide, benzthizide, bendroflumethiazide, chlorthalidone, metolazone, indapamide. Structure: most are related to sulfonamides. + Mechanism: Act on Na /Cl co-transporter at the distal convoluted tubule. Other actions: directly dilate arterioles, total fluid (extravascular) volume, cardiac output. Effects: + 1. urinary excretion of Na / water due to Na / Cl reabsorption + 2. urinary excretion of K and bicarbonate hypokalemia potassium dietary intake, use supplements / potassium sparing diuretics 3. blood glucose (hyperglycermia, care with diabetics), uric acid retention (hyperuricemia, care with gout), serum lipids (hyperlipidemia), calcium levels (hypercalcemia) 4. effect on other antihypertensives by re-expansion of extracellular / plasma volumes. SE: electrolyte imbalance (K, Mg, Cadehydration, postural hypotension, dizziness, headache, fatigue, hypovolemic shock, arrhythmia, palpitation), metabolic alkalosis, K muscle cramps, light sensitivity / rash (use sunscreen), uric acid / gout, lipoproteins, BG, sulfonamide hypersensitivity. Interactions: NSAIDs (e.g. ibuprofen) renal perfusion effect of thiazides. Sulfasenstivity. Hyperlipidemia risk of coronary artery disease. Digitoxin ( toxicity due to hypokalemia) urinary Ca excretion use for kidney stones (calcium nephrolithiasis). Metolazone: most effective thiazide diuretic. Chronic use water reabsoprtion polyuria and polydipsia in diabetes insipidus (ins tead of ADH) (??)
Osmotic diuretics
Examples: mannitol, glycerin, urea Mechanism: highly polar, water soluble inert chemicals, freely filtered at the glomerulus but poorly reabsorbed from renal tubules osmolarity of glomerular filtrate tubular reabsorption of water + diuresis water, Na , Cl , bicarbonate excretion alkaline urine.
Use: prevent oliguria, anuria, cerebral edema, intracranial pressure, intraocular pressure (glaucoma). SE: headache, blurred vision. Not absorbed well by the gut (causes osmotic diarrhea) only given IV.
Sympatholytics
Beta blockers
Use: recommended (with diuretics) as initial therapy, especially for patients with rapid resting heart rate (atrial fibrillation, tachycardia), ischemic heart disease (angina pectoris, MI) Mechanism: cAMP heart contraction and rate. Other: rennin secretion cardiac output, central in sympathetic output. Block autonomic reflex response (e.g tachycardia). Examples (x-olol): atenolol, , propranolol, timolol, acebutolol, betaxolol, bisoprolol, carteolol, metoprolol, nadolol, penbutolol, pindolol, esmolol, labetalol, carvedilol Nonselective B1 (heart) - B2 (lung) blockers: propranolol, nadolol, timolol. Selective B1 (heart) blockers: atenolol, metoprolol, acebutolol (A.M.A.), betaxolol, bisoprolol. Less likely to mask hypoglycemiause in DM. Intrinsic sympathomimetics (partial agonists, P.A.): pindolol, acebutolol, carteolol, penbutolol Labetalol: beta (1/2) and alpha-1 blocker (racemic mixture), for hypertensive crisis due to pheochromocytoma (tumor with catecholamines). SE: bronchospam, orthostatic hypotension, urinary retention. Carvedilol: beta (1/2) + alpha blocker and vasodilator. Timolol: mainly for ocular hypertension (B1/B2). Esmolol: ultrashort duration of action, IV. Carteolol: lipid solubility CNS penetration. Propranolol: -ve inotrophic/chronotropic oxygen demand angina Side effects, interactions, and precautions: Withdrawal syndrome if suddenly d/c anginal attacks, MI, rebound in BP above normal lipids, hypertriglyceridemia Impotence and libido compliance NSAIDs may effect of beta blockers SE with neurologic disorders if drug enters CNS poor memory, depression, fatigue, lethargy kidney blood flow glomerular filtration. Contraindications: Ca channel blockers CHF cardiac decompensation due to contractibility and electrical conduction DM may mask tachycardia (hypoglycemia), BG COPD, asthma, bronchospams (selectivity is dose dependent) Peripheral vascular disease / Raynauds phenomenon vasoconstriction
Vasodilators
Use: last line of treatment. Do not use alone (cause heart rate, heart output, plasma rennin). Directly relax peripheral vascular smooth muscles. Commonly used in hypertensive crisis (IV). General SE: tachycardia, headache, dizziness, fluid retention, nasal congestion. CI: coronary vascular disease the reflex cardiac stimulation (tachycardia) will myocardial oxygen demand. Diazoxide, Minoxidil potassium channel activators membrane hyperpolarization arteriolar vasodilation. Hydralazine NO (EDRF) arteriolar vasodilation. Hydralazine: dilates arteries (renal, cerebral). Triggers sympathetic compensatory reactions. SE: reflex (barorecceptor) heart rate/output (may cause angina), stroke volume, reversible systemic lupus erythematosus (SLE) fatigue, fever regular blood counts. Minoxidil: dilates arteries. SE: Hypertrichosis (used to treat male pattern baldness; alopecia), tachycardia reflex (give beta blocker), pulmonary hypertension. Diazoxide: dilates arteries. Quick and prolonged action. For hypertensive crisis. Nitroprusside: dilates arteries and veins. 44% cyanide. Mechanism: reacts with oxyhemoglobin (forms methemoglobin), forms nitric oxide which activates guanylyl cyclase. First choice for hypertensive crisis (IV, short duration). Use for controlled hypotension during surgical anesthesia (bloodless surgery, good cerebral perfusion). Also for heart failure (acute/chronic). Avoid in infants. Solution in water is susceptible to photolysis.
Dihydropyridines (nifedipine / nicardipine / nitredipine) vasodilation but no cardiac effects (no effect on SA / AV node) reflex sympathethetic response tachycardia. SE with SR form. Second generation dihydropyridine derivatives (related to nifedipine): amlodipine, isradipine, felodipine, nicardipine, nisoldipine. Chemically related to nifedipine. Selective effect on target tissues. Less reflex tachycardia. Nimodipine: lipid solubilityenters brainfor cerebral spasm
Hypertensive crisis
Definition: systolic > 200 or diastolic > 140 quick organ damage. Reduction of BP must be gradual (15 mmHg over first hour) to avoid compromising organ perfusion (esp. cerebral) Drugs: vasodilatos (nitroprusside, hydralazine, diazoxide, nicardipine, nitroglycerin), enalaprilat, adrenergic inhibitors (labetolol, esmolol, phentolamine (alpha blocker)), fenoldopam (dopamine D1 agonist, vasodilator), trimethaphan (ganglionic blocker)
Pathophysiology
CHF compensatory mechanisms to normalize cardiac output (stroke volume x heart rate) left ventricle geometry ventricular dilation, hypertrophy, cardiac wall thickness (cardiac remodeling).
Compensation
Sympathetic response: cardiac output sympathetic activation Ep, NEp heart rate, blood flow to vital organs (brain, heart). Hormonal stimulation: sympathetic blood flow redistribution renal perfusion glomerular filtration rate sodium / water retention, activation of renin-angioensin-aldosterone system more sodium retention, volume expansion. Concentric cardiac hypertrophy: ventricular remodeling. Frank-Starling mechanism: blood volume cardiac chamber stretch to accommodate excess fluid (distention) contractile force to expel fluid to the arteries.
Decompensation
Over time, compensatory mechanisms become exhausted and ineffective viscous cycle of compensation compensation become self-defeating. Afterload: tension in ventricular muscles during contraction, amount of force needed for the ventricle to overcome pressure in the artery, also called intravascular systolic pressure. Preload: force exerted on the ventricular muscle at the end of diastole that determines degree of muscle stretch, also called ventricular end diastolic pressure. As fluid volume demand on exhausted pump fluid backup symptoms of CHF.
Clinical evaluation
Symptoms are due to blood backing up behind the failing ventricle. Symptoms are first related to the failing side, then to both sides. Left-sided CHF Blood cant be pumped from the left ventricle to the peripheral circulation left ventricle cant accept blood from left atrium and lung blood back up in pulmonary alveoli pulmonary edema. Symptoms: dyspnea, less effort to trigger exertional dyspnea, wheezing cough, exertional fatigue, nocturia. Paroxysmal (sudden) nocturnal dyspnea and orthopnea result from volume pooling in the recumbent position relieved by propping with pillow or sitting upright. Physical findings: Crackles indicate air movement through fluid-filled passages, tachycardia (early compensatory mechanism). Diagnostic tests: cardiomegaly (heart enlargement), left ventricular hypertrophy, pulmonary congestion. Right-sided CHF Blood cant be pumped from the right ventricle to the lung right ventricle cant accept blood from right atrium and circulation blood back up in whole body systemic edema.
Symptoms: tightness and swelling (fingers, skin), nausea, vomiting, abdominal pain on exertion due to liver enlargement. Physical findings: vein distention due to venous pressure, tender enlarged liver, bilateral leg edema. Diagnostic tests: liver enzymes (ALT) due to liver congestion.
Therapy
Bed rest
Advantages: metabolic needs, heart workload, heart rate and dyspnea, diuresis fluid volume. Disadvantages: venous stasis thromboembolism, risk by using anti-embolism stockings, leg exercises.
Dietary controls
Small frequent meals with calories metabolic demand sodium (3g/d) to volume. Education patient about sodium containing products (antacids, NSAIDs, sodium bicarbonate, baking soda, water softeners).
Drug-related actions
ejection fraction can be achieved by: 1. Directly heart contractility using inotropic agents: dopamine, dobutamine, milrinone, amrinone. 2. resistance to ejection by relaxing peripheral blood vessels: vasodilators such as hydralazine, nitroprusside, nitrates 3. Affecting cardiac remodeling: ACE inhibitors, beta blockers, vasodilators (nitrates). Addressing the underlying problem is more important than symptoms.
Diuretics
Used for all CHF patients with fluid retention / edema. Monitor fluid loss and in edema by following body weight Thiazides: effective, commonly used. Disadv: weak, hypokalemia. Loop: v. effective, orally / IV for acute pulmonary edema. Hypokalemia. Potassium sparing: weak, balance the hypokalemia. Aldosterone antagonists: e.g. spironolactone.
ACE inhibitors
For long term, not acute, management of CHF. First line agents. Mechanism: enzyme for converting angiotensin I to angiotensin II (potent vasoconstrictor) total peripheral resistance afterload. angiotensin II also aldosterone release sodium / water retension venous return and preload.
Vasodilators
Mechanism: afterload (artery dilation) / preload (venous dilation) pulmonary congestion, cardiac output. Nitroprusside: IV, dilates both veins and arteries. Prazosin: alpha-1 blocker, dilates both veins and arteries. Hydralazine: dilates arteries. Nitrates: dilates veins. Higher dose for CHF than for angina.
Beta blockers
For long term, not acute, management of CHF. Only carvedilol (Beta-1-2-Alpha-1 blocker) is approved for CHF. Actions of norepinephrine: peripheral vasoconstriction, sodium retention by the kidney, cardiac hypertrophy, arrhythmia, hypokalemia, cell death (apoptosis) due to stress.
Prevention: nonpharmacologic ( venous stasis with external pneumatic compression or graduate compression stockings), pharmacologic (anticoagulant drugs or heparins).
Mechanism
Chemistry: Coumarin derivatives (warfarin, dicumarol) are water insoluble weak acids. Chemically related to vitamin K. protein bound. liver metabolism. therapeutic index. Therefore, drug interactions. Mechanism: antagonists of vitamin K. reductase responsible for interconversion of vitamin K and its epoxide liver production of defective () vitamin K-dependent coagulant proteins or clotting factors (2 (prothrombin), 7, 9, 10). Does not work in vivo.
PK
Warfarin is a racemic mixtuer of equal R/S forms Rapid absorption Cmax in 90 minutes. inter-individual variability in dose response. Used mostly orally, but also IV. Pregnancy X. Effect and depletion of clotting factors occurs after 3 day. Meanwhile, use UFH or LMWH if needed (5 day overlap). Effect also take time to wear off after d/c. Dose: 2.5-10 mg. Duration: 3-12 months.
Monitoring
Initial daily monitoring of prothrombin time (PT) and international normalized ratio (INR). Then frequency of monitoring gradually to every 4 weeks. PT results are highly dependent of type of reagent. INR = patient PT / mean lab control PT. Target: 2-3 (risk 2.5-3.5). ISI: International Sensitivity Index, a measure of thromboplastin responsiveness to in clotting factors. ISI responsive reagent PT ~ INR Warfarin is sensitive to metabolic enhancers / inhibitors, vitamin K. Antibiotics GI bacterial flora vitramin K warfarin toxicity. SE: hemorrhage / bleeding (treat with vitamin K, i.e. phytonadione IM/SC), skin necrosis (due to protein C), urticaria, purpura, alopecia.
Unfractionated heparin
Chemistry: large very acidic muco-polysaccharide molecule Indications: IV/SC with warfarin for proven VTED. Prevents / treats DVT, PE. Works in vivo to prevent clotting of blood samples. Avoid IM ( hematoma). Mechanism: inhibition / inactivation of thrombin (factor IIa, converts fibrinogen to fibrin clot), activated factor Xa (converts prothrombin II to thrombin IIa), by antithrombin (AT) III. PK: plasma proteins other than AT III compete for heparin binding. Short t1/2. Large molecule cant cross placenta safer in pregnancy. Clearance: combination of saturable and non-saturable first-order kinetic models. Involve rapid followed by gradual elimination. inter- and intra- individual variability (due to plasma proteins and clearance). Administration: start with a 70 units/kg loading dose for fast response, then continuous dose (1000 unit/hr or weight-based) SE: hemorrhage, thrombocytopenia (common), urticaria. Antidote: protamine sulfate (ver basic protein). Monitoring: measure activated partial thromboplastin time (aPTT) (patient aPTT / mean lab control aPTT) target: 1.5-2.5, but is dependent on the reagent. Heparin assay may also be used for monitoring.
Lepirudin
Chemistry: recombinant DNA (almost identical to hirudin). Mechanism: thrombin (factor IIa) thrombogenic activity (antithrombin). Use: anticoagulant in case of heparin-induced thrombocytopenia. SE: cerebral bleeding, allergic/ skin reactions.
Antiplatelet agents
Aspirin: Mechanism: dose permanent inhibition of COX thromboxane A2. Use: mortality post-MI, prevent MI reinfarction. Ticlopidine / clopidrogel: Mechanism: interfere with ADP-induced platelet-fibrinogen binding glycoprotein GPIIb/IIIa receptor. Use: MI, stroke risk. SE: , diarrhea, rash, GI upset, neutropenia. Fab fragments (Abciximab): Mechanism: monoclonal antibodies against GPIIb/IIIa receptor platelet interaction. Use: coronary angioplasty, atheroctomy. SE: bleeding, thrombocytopenia, antibody formation, arrhythmia. Eptifibatide / Tirofiban: Mechanism: same as Abciximab. Use: acute coronary syndrome, coronary angioplasty. Glycoprotein IIb/IIIa receptor antagonists fibrinogen, adhesion ligands. SE: bleeding, fever, headache. Dipyridamole: Mechanism: RBC adenosine, phosphodiesterase ( cAMP), thromboxane A2. Use: for thromboembolism prophylaxis after valve replacement. SE: nausea, GI upset, headache, rash, dizziness. Also relax smooth muscles, coronary vascular resistance (blood flow). Anagrelide: Mechanism: platelet production. Use: platelet count in thrombocythemia. SE: CHF, MI, heart block, arrhythmia. Cilostazol: Mechanism: PDE III inhibitors cAMP vasodilation. SE: CHF.
Thrombolytic agents
General Mechanism: conversion of plasminogen to plasmin (serine protease), which hydrolyzes fibrin and dissolves clots. General SE: bleeding (GI / GU / intracranial / catheter site), and allergic reactions (skin rash, bronchospasm, edema, urticaria). Alteplase / reteplase (t-PA): recombinant DNA-derived tissue plasminogen activators (t-PA) consisting of amino acids. Called Clot Selective because it acts on fibrin-bound plasminogen. SE: acute MI, acute pulmonary embolism. No allergy issues (human-derived) Streptokinase: protein derived from cultures of Group C beta-hemolytic streptococci ( hypersensitivity). fibrinogen and factors 5 & 8. Acts on bound & free plasminogen (not selective). Use: acute MI, DVT, arterial thrombosis. Anistreplase: also called Anisolyated Plasminogen Streptokinase Activator Complex, APSAC . Prodrug, activated in vivo by deacylation. Use: acute MI, coronary arterial thromobi. SE: arrhythmia, BP Urokinase: two-chain serine protease from cultured human kidney cells. Mechanism: enzymatically active (plasminogen plasmin). Use: coronary arterial thrombi, pulmonary embolism.
Etiology
Primary (idiopathic): called classic Parkinsons or paralysis agitans. Most common. Incurable disease. Can be due to absorption of highly potent neurotoxins (CO, manganese solvent, MPTP) or exposure to cell toxic hydrogen peroxide and free radicals; both products of dopamine catabolism. Secondary: small percentage, usually curable. Drugs: dopamine antagonists / antipsychotics (phenothiazines (chlorpromazine, perphenazine), haloperidol, reserpine). Toxins: CO, heavy metals (manganese, mercury, MPTP). Infections: syphilis, encephalitis. Others: Wilsons disease, arteriosclerosis. Pseudo-Parkinsons: due dose of older (traditional) antipsychotic agents, more in the elderly
Treatment:
Non-drug: Exercise / physical therapy: very beneficial for mobility and mood. Nutrition: to risk of poor nutrition, weight loss, muscle mass. fiber and fluid intake to prevent constipation. calcium to preserve bone structure. antioxidants (e.g. vitamin E) to oxidative stress. Psychological rehabilitation: support for patient, family. May need to treat depression, dementia. Drugs: TCA (anticholinergic, dopaminergic, for depression). Beta blocker (propranolol, lipid solubility), BZD, primidone for action tremor. Diphenhydramine: antihistamine with anticholinergic effect for mild tremor (CNS SE, avoid in elderly). Principles of therapy: if drug fails use another class, except bromocriptine and pergolide (try both in sequence). Build dose gradually up. Never d/c drug suddenly. Late disease disabilities: Levodopa motor fluctuation, dyskinesia, response control by changing dose and timing. Non-levodopa: urinary urgency oxybutynin, constipation fiber / PEG, salivation antihistamines / anticholinergics, sweating beta blocker / anticholinergic, orthostatic
hypotension desmopressin, pain amitriptyline, depression / dysphagia liquid levodopa, daytime sleepiness selegiline. Definitions: Dyskinesias: reversible jerky movements. On-off effect: oscillations in response and sudden changes in mobility from no symptoms to full symptoms within minutes. End-dose (wearing-off) effect: may improve by shortening the dosing interval. Drug holiday: temporary d/c of levodopa to reverse down-regulation of dopamine receptors and regain efficacy.
Individual drugs
Anticholinergic agents
Examples: benztropine, trihexyphenidyl (both structurally related to atropine), biperidene, procyclidine, orphenadrine. Use: mild symptoms, esp. tremors (not bradykinesia / pos. imbalance). Mechanism: block action of acetylcholine in basal ganglia. SE: dry mouth, sweating ( heat tolerance), urinary retention, constipation (use stool softener), delayed gastric emptying, intraocular tension, GI upset, dizziness, agitation, hallucinations, hypotension. CI: obstructed GI or GU, glaucoma, cardiac disease. Avoid drugs with anticholinergic activity (antihistamines, antidepressants, phenothiazines), digoxin level. Avoid combo with haloperidol ( tardive dyskinesia severeity, schizophrenia, haloperidol level).
CI: liver metabolism. pramipexole: cimetidine clearance. Ropinirole: smoking metabolism, ciprofloxacin metabolism.
Surgical treatment
Require needle insertion in the brain possible hemorrhage. Deep brain stimulation: implant frequency electrode into target site and connect lead to SC pace maker functional inhibition of target regions in the brain. Globus pallidus internus pallidotomy: surgical resection of parts of the globus pallidus. Retal nigral transplantation: implantation of embryonic dopaminergic cells to replace degenerated neuronal cells.
45. Schizophrenia
Pathophysiology
Genetic studies: 10x in risk with family history. 50% chance in both of monozygotic twins. Neurophysiologic theories: mainly due to dopamine. Serotonin and glutamate may play a role. Dopamine may in some brain areas. Psychosocial theories: may be triggers but not causes. Stress, interpersonal skills, bad family communications, socioeconomic factors. Population prevalence: 1%.
Diagnosis
Using Diagnostic and Statistical Manual (DSM) of Mental Disorders. Diagnosis by exclusion after ruling out medical and mental causes of psychosis. Symptoms: delusions, hallucinations, disorganized speech / behavior, negative symptoms (6 months + 1 month active symptoms causing social or occupational dysfunction). Types: paranoid (delusions of grandeur or persecution), catatonic (psychomotor disturbances), disorganized (incoherent responses), residual (history but no acute psychosis), undifferentiated.
No known cure. Objective is to relieve symptoms and restore function. Treatment: pharmacotherapy, psychotherapy.
Antipsychotics
Agent selection: based on patient history and drug safety. Atypical antipsychotics in new diagnosis or first episode (safer drugs). Antipsychotics are more effective for positive symptoms. Maximum effect: 6-8 weeks. One episode d/c gradually after 6 months. Multiple episodes: indefinite treatment.
Typical antipsychotics
Examples: phenothiazines (chlorpromazine, thioridazine, mesoridazine, fluphenazine, perphenazine, trifluperazine), haloperidol, loxapine, molindone. Mechanism: block dopamine (D2) activity. Cause hyperprolactinemia. Potency: potency extrapyramidal symptoms. potency sedation, anti-cholinergic, cardiovascular SE. Efficacy: as good as the typical drugs for the positive but not the negative symptoms. Generally, more SE than the typical drugs. Extra-pyramidal SE Acute dystonias: sudden muscle spasms (neck, jaw, back, eyes). Common in the first 2 days. Treatment: IV/IM anticholinergic (diphenhydramine, benztropine). Akathisia: motor restlessness, inner tension and agitation, urge to move (pacing). Common in the first weeks or months. Treatment; anticholinergic, beta blocker, BZD. Pseudoparkinsonism: parkinsonism induce by dopmine blockade. Common in the first weeks or months. Treatment: anticholinergic or switch to atyptical drug. Tardive dyskinesia (TD): latent extrapyramidal effect (after months / years). Abnormal movement (face, tongue, shoulders, hipds, extremities, fingers, toes, etc). Movements are fixed (dystonic) and rhythmic. Its due to prolonged dopamine blockade dopamine receptor up-regulation sensitivity to stimulation. Treatment: may be irreversible, d/c therapy, when dose symptoms may first worsen due to dopmaine blockade and still up-regulated receptors, dose may initially mask symptoms but will remerge later. Best approach is prevention (monitor). Neuroleptic malignant syndrome (NMS) Uncommon but sudden onset, serious and may be fatal. Symptoms: extrapyramidal effects, hyperthermia, tachycardia, BP, incontinence. Management: d/c drug, bromocriptine or dantrolene (muscle relaxant), supportive therapy.
Atypical antipsychotics
Examples: risperidone, olanzapine, clozapine, quetiapine. Block serotonin more than dopamione-2 receptors. Less extrapyramidal SE than typicals. No hyper-prolactinemia. Treat negative symptoms better than typicals. Clozapine: only drug with no EPS/TD. Only effective drug for refractory patients. However, use as last resort due to agranulocytosis (monitor CBC weekly). It has anticholinergic SE.
Other condiserations
SE by recptor type: histamine H1 sedation; serotonin 5-HT weight gain; dopamine D2 EPS / hyperprolactinemia; muscarinic anticholinergic / cognitive / tachycardia; alpha-1 orthostatic hypotension / reflex tachycardia. Rapid tranquilization: for acute psychosis with agitation and aggression. Use injectable typical drug (IM haloperidol). Noncompliant patient: use long acting IM drugs every 3 weeks; either haloperidol decanoate or fluphenazine decanoate. Convert existing oral dose to its injectable equivalent. Switching drugs: cross taper and titrate ( old, new). Adjunctive therapy: if 3 agent tried unsuccesfully use clozapine or augmentative therapy (BZD anixiolytics or modd stabilizers such as lithium, valproic acid or carbamazepine).
Major Depression
Incidence: more in women (2x men). 15-20% chance in womans lifetime. Etiology: Biogenic amine theory: due to depletion of serotonin and norepinephrine. Dysregulation theory: cyclic nature of depression is due to impaired balance of neutrotransmitters not absolute or . Familial history plays a role. Clinical depression: mood,anhedonia, appetite , weight , sleep , psychomotor , fatigue, worthlessness, guild, thinking / concentration, suicidal Diagnosis: using Diagnostic and Statistical Manual (DSM) IV criteria. Patient must have persistent symptoms for 2 weeks.
Treatment:
Psychotherapy, pharmacotherapy, and electroconvulsive therapy. Pharmacotherapy with antiderpssants is 50-60% effective. It has three phases: acute (6 wk, resolve symptoms), continuation (6-9 months, prevents relapse) and maintenance (3 or > years, prevents recurrence). Drug selection: all drugs are equally effective with different mechanisms and SE. Select drug with SE profile that complements the disease process. For example, depression with psychomotor agitation sedative antidepressant, depression with psychomotor retardation activating antidepressant. Therapy initiation: start with half of the lowest dose to minimize SE to target range in 1-2 weeks, then titrate based on response. GRADUAL. Lag time exists between therapy initiation and clinical response due to changes in postsynaptic receptor sensitivity. Resolution of anxiety and insomnia in 1-2 week. Full effect in 4-6 weeks. Serotonin syndrome: tremor, seizure, hyperreflexia, hypomania, agitation, fever, diarrhea, confusion. May occur when two serotonin enhancing drugs are used concomitantly or close to each other (e.g. MAOI, SSRI). Serotonin withdrawal syndrome: lethargy, myalgia, chills, dizziness, flu-like symptoms Tricyclic Amines (TCA) Examples: amitriptyline, nortriptyline, protriptyline, imipramine, trimipramine, desipramine, doexpin Serum concentrations are established for some drugs. Mechanism: blocks serotonin and norepinephrine reuptake. Also bind to cholinergic, histaminergic, alpha-adrenergic receptors (SE). SE: anticholinergic (blurred vision, dry mouth, constipation, urinary retention), alpha blockade (orthostatic hypotension), antihistamine (sedation, take at bedtime), seizure threshold, ECG changes, lethal if overdoes. Not first choice for depression. Other uses: neuropathic pain, insomnia. Monoamine oxidase inhibitors (MAOI) Examples: phenelzine, tranylcypromine, isocarboxazid. Mechanism: monoamine oxidase block break down of biogenic amines serotonin and norepinephrine in the brain Not first choice for depression. Only for depression with agitation, hypersomnia, anxiety. SE: orthostatic hypotension, weight gain, edema, sexual dysfunction. Isocarboxazid liver damage. May result in accumulation of sympathomimetic amines hypertensive crisis CI with decongestants, foods with tyramines (aged cheese, wine). MAO: 2 wk washout period before start or when D/C.
Bupropion (Wellbuterin) Mechanism: reuptake of epinephrine, serotonin, dopamine. SE: stimulation similar to SSRI give in the morning, seizure esp with eating disorders. Selective Serotonin Reuptake Inhibitors (SSRI) Examples: fluoxetine, norfluoxetine, sertraline, paroxetine, citalopram, demethylsertraline, fluvoxamine (for OCD). Mechanism: selectively block serotonin reuptake level SE: stimulation and insomnia (give in the morning), GI, sexual dysfunction, (weight gain?). Abrupt D/C serotonin withdrawal syndrome (except fluoxetine) D/C gradually. Metabolized by cytochrome P-450 drug interactions. Fluoxetine: norfluoxetine has long t1/2 5 wk washout period after D/C. Venlafaxine (Effexor) Mechanism: reuptake of epinephrine, serotonin, dopamine (similar to bupropion). CI: MAOI. Dose gradually. SE: nausea, GI (take with food), sustained hypertension (monitor BP). Trazadone Low dose is commonly used for insomnia with stimulating antidepressants. Mechanism: serotonin. SE: sedation, hypotension, GI Nefazodone Structure is similar to trazadone. Mechanism: serotonin. SE ( than trazadone): sedation, hypotension, GI, dry mouth. Interaction: protein binding interact with warfarin, phenytoin. Cytochrome P-450 inhibitor drugs metabolized by P-450. Mirtazapine Mechanism: presynaptic alpha-2 receptors norepinephrine and 5-HT central concentration. Specific affinity to 5-HT1 receptors SE compared to SSI (no insomnia, agitation, sexual dysfunction). Blocks H1 sedation, and 5-HT2c appetite. SE: sedation (take at bedtime), weight gain, dry mouth.
Bipolar disorder
Incidence: 1% of the population. More common in female teens or early 20s. Etiology: Family history in 90% (genetics). Due to imbalance and fluctuation in neurotransmitter levels. norepinephrine manic episode, norepinephrine depression. GABA (gamma-aminobutyric acid, inhibitory neurotransmitter) mania, due to unopposed excitatory neurotransmitters (norepinephrine, dopamine). calcium in CSF mania. calcium in CSF depression. G protein: involved in signal transduction and activation of other neurotransmitters. Hyperactive G protein mood instability. Glutamate binding to G proteins linked to NMDA is involved. Psychosocial and physical stressors trigger early episodes. Diagnosis: using DSM-IV and history of mania and depression. Mania: elevated, expansive or irritable mood for 1 week. Grandiose ideations, expansive self-esteem, sleep, racing thoughts, distraction, psychomotor agitation, dangerous activities. Mixed episode (mood incongruent): mania and depression symptoms. Bipolar I: manic or mixed episode. Bipolar II: depressive and hypomanic episode. Cyclothymia: depressive and manic symptoms for 2 years. Rapid cycling: four depressive, manic, hypmanic or mixed episodes in 12 months Clinical course: untreated episodes last days to months. Interval between episodes: 1-2 years. Episode sequence is unpredictable. Early onset bad prognosis.
Treatment
Acute, maintenance and continuation phases (like depression). Antipsychotics, antidepressants, and mood stabilizers may be used. Antipsychotics: short term therapy during acute mania to psychosis and agitation. Antidepressants: use for depression with suicidal tendency. Use cautiously to avoid triggering mania. Lithium First line therapy (except for mixed episodes or rapid cycling). Monovalent cation like Na and K. Citrate salt liquid, carbonate salt tablet. Food may delay absorption. Take with food to avoid rapid rise in serum concentration and SE. Highly distributed but takes 3 days delayed response. Eliminated through the kidneys with no metabolism. nd Mechanism: unknown. norepinephrine / serotonin, membrane stabilization, cAMP / cGMP (2 messengers). Dose: narrow therapeutic index. Can be used to acute mania ( and dose gradually, quick action for mania but slow for deperssion) or preventative maintenance (mania, depression). Require Cp monitoring. If high dose psychosis, psychomotor agitation give BZD or antipsychotics. SE: . Monitor Cp. Categorized into early, long term, and toxicity. Polydipsia, polyuria, nocturia, dry mouth, weight gain, libido, tremors, CNS. Toxicity: use emesis, gastric lavage, hemo- or peritoneal dialysis but not charcoal. st CI: renal failure, pregnancy 1 trimester. Interactions: drugs that serotonin serotonin syndrome. With BZD, antipsychotics neurotoxicit y. Valproic Acid (VA) Indications: anticonvulsant that works as a mood stabilizer. Can be used in acute episodes or as a mood stabilizer. Forms: elixir sodium valproate, capsules VPA, enteric coated tabs divalproex, injections sodium valproate sodium SE: . Monitor Cp. Blood (agranulocytosis, thrombocytopenia), weight gain, liver / pancreas damage, GI upset ( in divalproex). CI: sensitive to enzyme inhibitors and inducers. CBZ Indications: anticonvulsant that works as a mood stabilizer. Use in bipolar if lithium fails. nd Mechanism: modulate NEp and cAMP (G protein-linked 2 messenger system). SE: CNS: drowsiness, dizziness, blurred vision, diplobia, nystagmus, confusion, headache. Dose related: blood dyscrasias, dose gradually to avoid SE, GI upset (take with food). Non dose related: skin SE. Metabolic enzyme inducer (drug interactions, monitor Cp). Complete monitoring (blood count, live function, BUN, electrolytes, TSH). New Mood stabilizers (anticonvulsants) (gabapentin, lamotrigine) Indications: both mood elevation during epilepsy. Not approved, though, for mood stabilization (no systematic data). Lamotrigine: structure is similar to phenytoin and CBZ. Mechanism: block sodium-mediated release of glutamate and aspartate, may also block GABA and Ach release. SE: dizziness, blurred vision / diplobia, GI upset, rash / photosensitivity. Gabapentin: structure is similar to GABA (but no effect on GABA). Mechanism: unknown. dose gradually. Short t1/2 frequent administration. SE: somnolence, dizziness, nystagmus, fatigue.
Other topics
Use of dual mood stabilizers Combination of lithium and CBZ or VPA. Watch for leukocytosis / leukopenia. Do not combine CBZ and VPA ( blood dyscrasias). May also combine one of the three (older drugs) with one o f the two newer drugs (above).
Mood stabilizers in pregnancy nd rd Older drugs (lithium, VPA, CBZ) may cause birth defects. If necessary, use lithium only in 2 and 3 trimester. If necessary, give folic acid with VPA to risk.
Etiology
Allergens (pollen, dust mite, animal dander, mold, food), occupational exposures (chemicals, flour, wood, textile dust), viral respiratory infections, exercise, emotions (anxiety, laughter, stress, crying), irritant exposure (odors, chemicals, irritants), environmental exposure (weather change, cold air, smoke, sulfer dioxide), drugs (hypersensitivity, aspirin, NSAID, cholinergics (bethanechol), anti-adrenergics (B blockers)). Allergic rhinitis is twice as common in asthmatics.
Pathology / pathophysiology
Postmortem examination: smooth muscle hypertrophy, airway plugs (inflammatory cells, debris, proteins, mucus), vessel vasodilatation, inflammatory cellular infiltrate, collagen deposition. Major contributing processes Inflammatory cells: such as mast cells, eosinophils, activated T cells, macrophages, epithelial cells secrete mediators. Airway obstruction: due to bronchoconstriction, airway wall edema, mucus plug formation, airway remodeling, smooth muscle hypertrophy, hyperplasia. Obstruction ventilation ventilation / perfusion (V/Q) imbalance hypoxemia and partial pressure of arterial oxygen (PaO2). Hyper-responsiveness: response to stimuli due to inflammatory mediators and infiltration by inflammatory cells. Airway inflammation: contributes to hyper-responsiveness, obstruction, respiratory symptoms, muco ciliary function, airway permeability to allergens / irritants. Autonomic neutral control: cholinergic sensitivity parasympathetic tone, reflex bronchoconstriction. Airway remodeling: due to persistent inflammation in poorly controlled asthma collagen deposition and fibrosis permanent airway abnormalities. Sequencing of events in asthma Triggering: exposure to trigger (allergen, aspirin, virus, etc) antigen binds to IgE attach to activated mast cells. Early response: begins in < 30 min and resolves in < 2hr, blocked by beta agonist or cromolyns. Late response: begins 6 hr after trigger, persistent airway obstruction, inflammation, hyperresponsiveness, occurs in 50% of cases, may last several days, blocked by corticosteroids or cromolyns. Signaling: inflammatory cells (mast cells, lymphocytes, eosinophils, macrophages, epithelial cells) release chemical signals (cytokines, chemokines, eicosanoids, leukotrienes) attract more inflammatory cells. Migration: influx of inflammatory cells (eosinophils, lymphocytes, monocytes, granulocytes); adhesion molecules attract cells to the airways. Cell activation: required before cells can release inflammatory mediators. Eosinophils activation inflammatory mediators smooth muscle constriction, initiate chemotaxis. Leukotrienes
bronchoconstriction, mucus, vascular permeability, responsiveness. Other mediators recruit more inflammatory cells to the airways in the late asthmatic response. Tissue stimulation and damage: due to release of inflammatory mediators from activated cells. Epithelial damage airway responsiveness may cause remodeling.
Clinical evaluation
Physical findings Acute exacerbations: occur suddenly or gradually, usually at night or early morning. Shortness of breath, tachypnea, tachycardia, wheezing at end of exhalation, chest tightness, cough. Chronic poorly controlled severe asthma: chronic hyper-inflation, barrel chest. Signs of respiratory distress: cyanosis ( PaO2 / PaCO2), use of accessory muscles, inability to speak in sentences, mental status, PEFR < 50% of normal. Potentially fatal asthma: history of sudden severe exacerbations, poor self-perception of asthma, history of intubation or ICU admission, visits to ER or hospitalization for asthma, frequent beta agonist use (>2 canisters / month). Diagnostic tests Pulmonary function tests: determine degree of obstruction, may be normal between exacerbations. Forced expiratory volume in 1 second (FEV1): during exacerbation. Air trapping and lung hyperventilation residual volume (RV), total lung capacity (TLC). Peak expiratory flow rate (PEFR): correlates with FEV1, used to monitor therapy, triggers, need for emergency care. Measure PEFR in early morning before medications, and may be again midday. Diurnal variation > 20% in PEFR indicate responsiveness, and poor control. Blood analysis: WBC count during acute exacerbation, eosinophilia, leukocytosis (due to WBC demargination due to corticosteroids). Sputum analysis: may reveal eosinophils, clumps of epithelial cells, bacterial if infected, mucous in small airways. Pulse oximetry: noninvasive measure of degree of hypoxemia during acute exacerbation. It measures oxygen saturation in arterial blood (SaO2) and pulse. Arterial blood gas: help gauge the severity of exacerbations. Early stages hyper-ventilation PaCO2 fatigue of respiratory muscles. Respiratory acidosis: poor prognostic sign respiratory fatigue respiratory rate PaCO2. ECG: may show sinus tachycardia, especially in the elderly. Chest radiograph: may show pneumonia, hyperinflation. Allergy skin and radioallergosorbent test: identify possible allergic triggers.
Complications
Status asthmaticus: severe asthma exacerbation that fails to respond to therapy life threatening. Symptoms: consciousness, cyanosis, PaCO2, PEFR < 100 L/min or FEV1 < 1 liter. Treatment: oxygen, inhaled beta agonist, anticholinergic, IV steroids. If respiratory acidosis tracheal intubation, mechanical ventilation. Pneumothorax: acute exacerbation with air accumulation in the pleural space. Symptoms: chest pain, dyspnea, cough, anxiety, lung collapse. Treatment: oxygen, pleural air aspiration, analgesics. Atelectasis: airway obstruction gas exchange during respiration collapsed lung. Symptoms: worsening dyspnea and anxiety, hyperventilation, breath sounds, cyanosis. Treatment: postural drainage, chest percussion, coughing / breathing exercise, bronchodilators, bronchoscopy to remove secretions.
Therapy principles
Acute exacerbations At home: depends on EFV or PEFR. If < 50% of personal best aggressive treatment. Limit inhaled albuterol to 3 treatments of 3 buffs by MDI at 20 min intervals or one nebulizer treatment. If response is poor use oral corticosteroid, go to ER if needed. In the hospital: inhaled albuterol, mechanical oxygen ventilation (up to 90% saturation), anticholinergic, oral or IV corticosteroids, intubation.
Persistent asthma Step-down approach: aggressive. Start treatment one step above assessed severity for rapid control, review every 3 months. Then, do gradual step-wise reduction in treatment. Step-up approach: start treatment at the same step as assessed severity, and adjust upward as needed. Always, control environment to avoid triggers. If daily or use of inhaled albuterol consider long-term therapy (e.g. anti-inflammatory). A rescue course of systemic corticosteroids may be used. Exercise-induced bronchospasm (EIB) Warm-up period helps prevent EIB. Prevent EIB by using short acting beta agonist (albuterol) 15 min before exercise, long acting beta agonist (salmeterol) 45 min before exercise, or cromolyn sodium 1 hr before exercise. Keep albuterol handy. Chronic asthma (NIH guidelines) Severe persistent: dose inhaled steroid + dose oral steroid + long acting bronchodilator (inhaled or oral salmeterol, SR theophylline). Moderate persistent: inhaled steroid + long acting bronchodilator for nigh time symptoms (inhaled or oral salmeterol, SR theophylline) (drop oral steroid). Mild persistent: only one of the following: dose inhaled steroid, inhaled cromolyn, SR theophylline, leukotriene modifier. Mild intermittent: no daily medications. Albuterol for attacks.
Therapeutic agents
Beta agonists Short acting: albuterol (R- and S- isomers), levalbuterol (only active R-enantiomer), metaproterenol, pirbuterol, for acute exacerbation and EIB prophylaxis. Long acting: salmeterol, formoterol for asthma maintenance, EIB prophylaxis, nocturnal symptoms, albuterol use, COPD. Mechanism: stimulate beta 2 receptors adenyl cyclase cAMP bronchodilation, mucociliary clearance, inflammatory cell mediator release. SE: tremors (due to B2 activation in skeletal muscles), gluconeogenesis ( glucose), activation of Na K ATPase, cardiac stimulation (due to partial B1 stimulation: palpitation, tachycardia), nervousness, headache. Administration: inhalation systemic SE (preferred over oral). Always use salmeterol with in haled steroid, except for EIB prophylaxis. May combine long and short acting. Tachyphylaxis: occurs due to regular use. Its due to down-regulation due to moving of beta receptors from cell surface to inside the cell. Effect may be reversed with steroids. Paradoxical bronchoconstriction: due to cold-Freon effect or use of adjuvants. bronchial hyperactivity: due to irritants such as methacholine and histamine. May be due to albuterols S-isomer. Drug interactions: hypertensive crisis with MAO inhibitors, TCA and methyldopa. Beta blockers (e.g. propranolol) bronchospasm. Combined with sympathomimetics heart effect, vasoconstriction (prevent by alpha blockers, phenolamine). Corticosteroids Mechanism: Bind to glucocorticoid receptors in the cell cytoplasm alter gene transcription inflammatory response, airway hyper-responsiveness, mucus. Use: in case of allergic component. Added only when anticholinergic / beta agonist combo is ineffective. Systemic steroids: used for rapid response during acute exacerbations (few hours). IV steroids: hydrocortisone and methylprednisone. Alternative to oral steroids to prevent respiratory arrest in hospitals. Switch to oral steroids after stabilization. Oral steroids: prednisone, prednisolone. Used in emergencies if possible when there is no risk of respiratory arrest. Used in burst doses for a week. Dose tapering may be required. Inhaled steroids: fluticasone, flunisolide, triamcinolone, beclomethasone, budesonide. Used for chronic treatment, not for acute exacerbations. Less SE and less efficacy. steroid penetration into bronchial tree by giving bronchodilator several minutes prior.
Systemic steroids SE: hyperglycemia, BP, CHF, peptic ulcer, immunosuppression, chronic infections, osteoporosis, glaucoma, depression, psychosis, cataract, skin changes. If long term, minimize SE by giving morning dose or alternate day dosing. Inhaled steroids SE: fungal infection, voice hoarseness, dry mouth. May children growth velocity, but uncontrolled asthma also retards growth. Systemic SE with large doses. Gargle and wash mouth after use to fungal infections, systemic absorption. Interactions: enzyme inducers (rifampin, barbiturates, hydantoins) steroid metabolism. Oral contraceptives, estrogens, enzyme inhibitors steroid clearance. hypokalemia with thiazide and loop diuretics, amphoterecin digitalis toxicity. Cyclosporine steroid concentration. Leukotriene modifiers Leukotrienes: derivatives of fatty acids formed by lipoxygenase. No ring structure. Covalently linked to 23 amino acids. Slow reacting substances of anaphylaxis. eosinophil and neutrophil migration, leukocyte adhesion, neutrophil and monocyte aggregation, capillary permeability, smooth muscl e contraction, mucous secretion, bronchoconstriction, . Effect: anti-inflammatory and bronchodilation steroid dose. Leukotriene receptor antagonists (x-lukast) Examples: zafirlukast, montelukast Mechanism: prevent interaction of leukotrienes with receptors by cysteinyl leukotriene-1 block effect of histamine in asthma and allergy reactions. Take zafirlukast on empty stomach (max absorption). SE: , can be used in children. GI upset, dizziness. Churg-Strauss syndrome: eosinophilic vasculitis angiitus when steroids are d/c or . DI: enzyme inhibitor, effect of warfarin / theophylline. Lipoxygenase inhibitor (Zileuton) Mechanism: blocks 5-lipoxygenase leukotrienes synthesis from arachidonic acid. SE: liver dysfunction and ALT (m onitor, esp in alcoholics). Others (mild): headache, GI upset, myalgia. DI: effect of warfarin, theophylline, propranolol. Mast cell stabilizers (Cromolyn, nedocromil Na) Effects: Nonsteroidal anti-inflammatory. Less effective than steroids. Used only for asthma maintenance, EIB prevention. Mechanism: mast cell degranulation, inflammatory cells. SE: , used in children. Wheezing, coughing, nasal congestion, throat irritation / dryness. Methyl xanthines (theophylline) Use: alternative to B-agonists and steroids in acute attacks and to long acting B-agonist in persistent asthma. Combine with inhaled steroids control night or early morning symptoms. Effects: mucus, mucociliary transport, respiration, anti-inflammatory, renal diuresis. Mechanism: phosphodiesterase cAMP, antagonize adenosine receptors. Less bronchodilation than B-agonists. Oral (SR): compliance. fat tissue distribution, calculate dose based on lean body weight. Gradually titrate dose upward. IV: rare. Start with loading dose, then maintenance infusion. Theophylline anhydrous oral solids, theophylline monohydrate oral solutions. Aminophylline IV. SE: palpitations, restlessness, nervousness, insomnia, seizures, GI upset, diarrhea, dizziness. Do not use in pregnancy. Therapeutic drug monitoring: monitor SE, serum level, other drugs use. Clearance is age and condition specific. Interactions: multiple drug and other interactions. clearance ( level) with smoking, protein. clearance ( level) with age ( or ) , fats and carbohydrates, CHF. CI: peptic ulcer or uncontrolled seizure. Anticholinergics Postganglionic muscarinic block bronchodilation.
Use: more effective in COPD than in asthma. Ipratropium sodium: quaternary ammonium compound. Used with or as an alternative to beta agonist in acute attacks. Slow onset and long duration compared to beta agonists give regularly. SE: intraocular pressure if touches the eye, anticholinergic. Atropine aerosols, glycopyrrolate (quaternary ammonium compound): rarely used due to SE and efficacy. Used in nebulizers Other drugs Antihistamines: if patient has allergic rhinitis. Prevent release of histamine mediated response that influence asthma. Antibiotics: used to treat infections (change in volume, color, viscosity of sputum). Sputum cultures are useless because COPD are chronically seeded. Chronic antibiotic preventative used can be considered in case of frequent exacerbations. M. pneumoniae or Legionella pneumophilia macrolide . C. nd rd pneumoniae oral doxycycline. Pneumonia in the hospital 2 or 3 generation cephalosporin or beta-lactam with b-lactamase inhibitor. Magnesium sulfate (IV): cause little bronchodilation, respiratory muscle strength in hypomagn esemic patients. Immunotherapy: may lung function, symptoms. Non-pharmacologic Humidified O2: flow rate helps reverse hypoxemia (use if PaO2 < 55 mmHg), esp. at night/during exercise. Goal: SaO2 > 90%. Heliox: helium / oxygen mixture that is less dense than air ventilation during acute attack. IV fluids: and electrolytes are given if volume is depleted. Environmental control: avoid allergens and triggers. Use allergen-resistant mattresses / pillow encasements, filtration vacuum cleaners, avoid ferry pets, carpets and draperies. Vaccines: used to prevent infections that may trigger asthma (e.g. influenza and polyvalent pneumococcals). Drug delivery options MDIs: accurate with good technique and a spacer. A facemask may be needed for children. Wait 1 min between buffs. Spacers and holding chambers: drug deposition in the upper airway, oral absorption, local / systemic SE. Spacers are important for dose steroids or if hand-lung coordination is poor. Nebulizers: require patient coordination. Disadvantages: cost, time consuming, size, inconsistent drug delivery. Used in dose beta agonists, anticholinergics, cromolyn in children. Dry powder inhalers: more common, avoid the use of Freon propellants, easier to use. First load the dose, and then inhale rapidly. No spacers. Keep away from moisture.
COPD
Chronic bronchitis
Definition: excessive mucus production by the tracheo-bronchial tree edema and bronchial inflammation airway obstruction. Pathophysiology: respiratory tissue inflammation vasodilation, congestion, mucosal edema mucus. Neutrophils infiltration. Cilia impairment. Cartilage atrophy. Airways become blocked by thick, tenacious mucus secretions sputum rich productive cough. Normally sterile airways become colonized by Strept pneumoniae, H influenza, Mycoplasma. Recurrent viral / bacterial infections body defenses, mucus accumulation, ciliary activity. Airway degeneration gas exchange exertional dyspnea. Hypoximia, PaCO2 (hypercapnia). Physical findings: chronic productive cough after age 45 (first in winter, worse in the morning). Progressive exertional dyspnea, obesity, wheezing, prolonged expiration, right ventricular failure, cyanosis (called blue bloater) Diagnostic tests: hypoxemia erythropoiesis polycythemia ( RBCs). WBC due to infections. Sputum: thick, colored (if infected), neutrophils, microorganisms. Arterial blood gas: PaO2 (hypoxemia), PaCO2 (hypercapnia). FEV1. Right ventricular hypertrophy and cor pulmonale in ECG.
Emphysema
Definition: permanent alveolar enlargement and destruction of the alveolar walls, alveolar surface area. Pathophysiology: Inflammation, mucus secretion alveoli air trapping. tissue damage space into which normal lung tissue expands.. Alveoli merge space for air trapping. Alveolar wall destruction small airways collapse. Hypercapnia and respiratory acidosis are uncommon because of compensatory in respiratory rate. Physical findings: cough is chronic but less productive than in chronic bronchitis, starts at age 55. Exertional dyspnea is progressive, constant, more severe than in bronchitis. Other findings: weight loss, tachypnea, prolonged expiration, breath sounds. Patient usually maintain good oxygenation through tachypnea pink buffer. Diagnostic tests: small chance of AAT in blood or infections in sputum. PaO2 and PaCO2 in arterial blood gas, FEV1.
Etiology
Smoking: causes pulmonary hyperactivity and persistent airway obstruction. Alpha-1 antitrypsin (AAT) is a serine protease inhibitor neutrophil elastase. risk of COPD when smoking is combined with genetic ATT deficiency. Others: exposure to irritants (sulfur dioxide, polluted air, noxious gases, dusts), family history, social, economic factors.
Complications
Pulmonary hypertension: lung congestion pulmonary vascular bed space pulmonary hypertension cor pulmonale (right ventricular hypertrophy) right heart failure. Acute respiratory failure: advanced emphysema brain respiratory center damage cerebral oxygenation PaCO2 hypoxia, respiratory acidosis respiratory failure. Infection: chronic bronchitis trapping of excessive air, mucus, bacteria and coughing and deep breathing infection. Polycythemia: in RBCs hypercoagulate state, embolism, stroke.
Therapy
Anticholinergics: First line treatment for COPD. Beta blockers, corticosteroids, theophylline, O2, etc (see above) Mucolytics: such as acetylcysteine sputum clearance, mucus plugs. May cause bronchospasm. Expectorants: such as guaifenesin. Avoid potassium iodide. Chest physiotherapy: loosens secretions, re-expand lungs, efficacy of respiratory muscle. More important in outpatient. Physical rehabilitation: exercise tolerance and diaphragm and abdominal muscle tone. Smoking cessation: and avoidance of irritants. Use drugs with behavior intervention for maximum success. Surgery: lung volume reduction therapy
Classification:
Four of the following criteria have to be met 1. Morning stiffness for 1 hour before improvement 2. Three joints have fluid or soft tissue swelling 3. One joint in the hand joints must be swollen. 4. Symmetric arthritis: involvement on both sides of the body.
5. Subcutaneous (rheumatoid) nodules 6. serum rheumatoid factor 7. Radiological erosion or decalcification of bones May also include extra-articular organ manifestations (GI, infections, etc)
Etiology
Human leukocyte antigen (HLA-DR4) + environmental factor inappropriate immune response chronic inflammation Tumor necrosis factor (TNF) in RA and Crohns disease. Infections may ppt RA in predisposed patients, e.g., polyarthritis with lyme disease
Pathogenesis
Vasodilation, edema, sensation of heat, loss of function, production of thick boggy synovial fluid, effusion accumulation. Pannus: exuberant synovial thickening due to inward overgrowth of enlarged synovium across the surface of articular cartilage cartilage degradation, bone loss, x-rayed marginal erosions, bone rubbing, pain.
Clinical course
Variable and unpredictable, polycyclic course (intermittent remissions) or progressive course (relentless rapidly advancing destructive deforming inflammation permanent join deformities progressive functional decline, range of motion, work disability, loss of 4 -10 years of life expectancy. Early symptoms: aching, joint pain, fatigue, then hand and feet synovitis (swelling, warmth, tenderness). Morning stiffness: maximal pain and stiffness on awakening (30 min)
Therapy
Mechanical therapy
A balanced daily program of rest and exercise ( muscle strength and joint motion). Use lightweight splints during night (or even day) to align joints. Avoid complete immobilization. Consider joint replacement.
Other NSAID Examples: naproxen, ibuprofen, sulindac, piroxicam. May be better tolerated than aspirin. Try for 2 weeks before change. Chemistry: x-en propionic acids, others acetic acids. Avoid in asthmatics may trigger bronchospasm. bleeding time / platelet function (effect reverse quickly if d/c) GI upset, ulceration, hemorrhage ( platelets). GI ulcers by using misoprostol (Cytotec, SE: diarrhea) or H2-antagonists. Ibuprofen, naproxen GI SE available OTC. Piroxicam GI SE, CI in elderly. renal blood flow renal failure (esp with diuretics or CHF). Temporary CNS effects (headache, drowsiness, confusion, anxiety, etc) esp. with indomethacin. Avoid in the elderly. Meclofenamate: diarrhea COX-2 inhibitors Rofecoxib, celecoxib, valdecoxib. Anti-inflammatory, analgesic, antipyretic with GI SE.
Mycophenolate mofetil: immuno-suppressant. SE: diarrhea, GI, hematologic. Used to prevent cardiact and renal allograft rejection. Other drugs: chlorambucil, cyclosporine, minocycline. Corticosteroids Prednisone. Last resort. They do not alter the course of RA. Used for acute flare ups, before action of slow acting drugs kicks in, systemic RA symptoms, or in case of intolerance to other drugs. Can be used as intra-articular injection if symptoms are localized. SE: GI bleeding, slow wound healing, hyperglycemia, hypertension, osteoporosis.
Topical therapy
Capsaicin: for symptomatic treatment. Its the pungent ingredient of hot pepper. Mechanism: depletes and prevents accumulation of substance P, a chemical mediator in pain transmission from the periphery to CNS (sensory nerve fibers). It produces a sensation of warmth. Use: joint pain, arthritis tenderness, neuralgia, psoriasis. SE: erythema (reflex vasodilation), histamine release. Counter-irritants: methyl salicylate, menthol, allyl isothiocyanate, produce a mild inflammatory reaction. Effect may be actually due to the massage during application not the drug itself.
Etiology
Primary: due to defect in purine metabolism or uric acid excretion. It is due to uric acid production or renal clearance or both. Under-excretors (90%): excrete < 600 mg/day on a purine restricted diet. Secondary: renal failure ( excretion), hematologic diseases ( nucleic acid breakdown to uric acid). Drug induced gout: Ethanol production and secretion. Aspirin and salicylates uric acid tubular secretion ( excretion). Diuretics (except spironolactone)volume depletion / tubular secretion. Cyclosporine, pyrazinamide, levodopa urate renal clearance. Ethambutol, nicotinic acid compete for urate secretion excretion Cytotoxic drugs nucleic acid turnover.
Pathophysiology
Gouty arthritis develop when monosodium urate crystals deposit in the join synovium inflammatory response gout attack join swelling, redness, warmth, tenderness tophi (urate deposits) joint deformity, disability, renal impairment. Renal complications: Acute tubular obstruction: due to uric acid pptn in the ureters and collecting tubes. Urolithiasis: uric acid stones due to low urine pH. Chronic urate nephropathy: urate deposits in the renal interstitium.
Therapy
Immobilize affected joints. Start anti-inflammatory drugs immediately. Start urate-lowering drugs after attack is over. Colchicine: drug of choice for pain and inflammation and ending the attack. Mechanism: antimitotic, chemotaxis of leukocyte to inflamed area, phagocytosis and urate deposition. Orally or IV (never IM or SC due to irritation). SE: diarrhea, GI, bone marrow depression, irritation if given IM. NSAIDs: if first choice is colchicine is not tolerated or not started immediately. Examples: indomethacin, naproxen, sulindac. SE: GI, CNS headache and drowsiness / dizziness. Take with food. Aspirin dose uric acid secretion, dose uric acid secretion. Corticosteroids: Methylprednisolone acetate given intra-articular with diagnostic / therapeutic aspiration. Prednisone (oral), Triamcinolone acetonide (IM) or methylprednisolone (IV).
Intercritical gout
Symptom free period between attacks. Non-drug urate lowering: high-purine diet (meats, legumes), obesity, alcohol. Limited effect. Prophylaxis: dose colchicine or NSAID. Urate lowering therapy (<6 mg/dl): lifelong treatment. Allopurinol (isopurine): production. Mechanism: xanthine oxidese ( xanthine hypoxanthine uric acid). Long acting active metabolite: oxypurinol. Preferred over uricosurics in case of renal impairment ( dose). SE: reversible rash ( incidence with ampicillin), exfoliative dermatitis treat with prednisone, Stevens-Johnson syndrome. May gout attacks if given during the attack due to mobilization of stored urate (give colchicine). May dissolves tophi. Uricosurics excretion. Examples: sulfinpyrazone, probenecid (benzoic acid derivative). Mechanism: uric acid reabsorption at the proximal convoluted tubules. Do not initiate during acute attacks or give with colchicine. During the first 6-12 months may attacks. Maintain fluid intake, urine output and alkaline urine to risk of renal urate pptn. Build up dose gradually. Action is antagonized by salicylates. SE: GI, blood dyscriasis (sulfinpyrazone). CI: urinary tract stones. Chronic tophaceous gout: urate pool. Large SC tophic. Allopurinol / probenecid combo.
Gastro-esophageal reflux disease (GERD): retrograde movement of gastric contents from stomach into esophagus. When reflux leads to inflammation and/or ulcerations, its called reflux (erosive) esophagitis. Dyspepsia: persistent / recurrent, pain / discomfort in upper abdomen.
Manifestations
Duodenal ulcers: develop in the first cm of duodenum (bulb). Gastric ulcers: common in the antrum or antral-fundal junction. Stress ulcers: from serious trauma or illness, major burn, sepsis. Zollinger-Ellison syndrome: severe peptic intractable ulcer with extreme gastric hyperacidity and gastrionoma (non-beta islet cell tumor). Diagnosed by fasting plasma gastrin concentration. Stomal (marginal) ulcers: after ulcer surgery or subsequent ulcer recurrence after symptom free period. Drug-associated ulcers: chronic ulcerative drug users (e.g. NSAIDs) Reflex esophagitis: recurrent symptoms (heartburn), altered epithelial morphology. Heartburn may radiate to the neck. Other symptoms: belching, chest pain, asthma, cough, hoarseness, laryngitis. Epidemiology: Duodenal ulcers: 7% incidence. Gastric ulcers: 0.05%. May have both gastric and duodenal ulcers. Onset: 30-50 years. 45% of the population experience heartburn once a month. 15% take indigestion drug twice a week. Prevalence of dyspepsia: 25% (3% of doctor consultations). Hospitalization / mortality for peptic ulcer are . Description: Duodenal ulcers < 1 cm diameter. Gastric ulcer: slightly larger. Edges are sharply demarcated. Surrounding mucosa is inflamed and edematous. Scar may form after healing. Gastric ulcers may be malignant (10%).
Etiology
Helicobacter pylori (campylobacter pylori): gram negative spiral bacteria with multiple flagella living in the gastric mucosa. Produces urease hydrolyzes urea into ammonia neutralizes gastric HCl bacteria survives. H pylori prevalence with age. 15% of positive persons develop ulcer. H pylori is present in 90% of gastric and duodenal ulcer and cancer cases. Eradication may help ulcers and dyspepsia. Genetics: ulcer prevalence with first degree relative is 3x the normal rate. May be due to H pylori presence. Blood type O have incidence. + NSAIDs: chronic use COX-I PG synthesis (cytoprotective to mucosa). Also, allow H back diffusion into mucosa injure mucosa Smoking: incidence of ulcer, ulcer healing and incidence of relapse. Nicotine biliary and pancreatic bicarbonate secretion, stomach emptying into the duodenum. Alcohol: known mucosal irritant, especially at concentrations > 20%. Coffee: peptides in regular and decaf coffee gastrin release gastric juice flow. A direct coffeeulcer link is not proven. Associated disorders: incidence with hyper-parathyroidism, emphysema, rheumatoid arthritis, alcohol cirrhosis. Advanced age: pylorus degradation bile reflux into the stomach ulcers. Corticosteroids: NO link between corticosteroids and ulcers. Psychological factors: minor factor, contrary to the opposite belief.
Pathophysiology
Ulcers occur due to imbalance between factors protecting gastric mucosa and factors causing mucosal corrosion. Protective factors: thick mucosal mucus is a barrier between luminal acid and epithelial cells. This barrier inward movement of hydrogen ions and allow neutralization by bicarbonate ions in fluids secreted by the stomach and duodenum. Alkaline and neutral pancreatic biliary juices buffer acid entering duodenum from the stomach. Corrosive factors: gastric mucosa is unable to resist corrosion by irritants such as HCl and pepsin. Mucosal barrier may not be intact. Physiologic factors: Duodenal ulcer: gastric emptying rate, post-prandial acid secretion, serum pepsinogen I, pepsin secretion, # of acid producing parietal cells. Gastric ulcer: gastric emptying rate, mucosal resistance, serum gastrin, mucosal PG.
GERD: reflux occur via transient lower esophageal sphincter relaxation (TLESR). People with GERD TLESR frequency. Dyspepsia: caused by PUD, GERD, gastric cancer, biliary tract disease.
Clinical presentation
Only 50% of patients experience classic ulcer symptoms. Pain: heartburn, aching, burning, cramping. May be due to chemical stimulation or spasm. Duodenal ulcer pain: more localized and often peaks between 12-2 AM. Gastric ulcer pain: less localized. Food: may duodenal ulcer pain but gastric ulcer and GERD pain. So, duodenal ulcer patients may gain weight and gastric ulcer patients may lose weight. Pain occurs 1.5-3 hr after meals in duodenal but only 1 hr after meals in gastric ulcer. Disease course: usually chronic with remissions and exacerbations. Relapse may be more common in spring and autumn. Test for and eradicate H pylori and use maintenance drugs to recurrence.
Clinical evaluation
Blood test hypochromic anemia. Stool test occult blood in chronic ulcers. Gastric secretion tests hyper-HCl secretion in duodenal ulcers, normal or subnormal HCl secretion in gastric ulcer. Upper GI barium x-ray: reveals ulcer crater. Upper GI endoscopy: most conclusive test. Biopsy: may be necessary to detect malignancy. H pylori status: using non-invasive (serology or breath test, false negative breath test with PPI, antibiotics or bismuth compounds) or invasive (histological bacterial visualization or urease activity test) tests.
Complications
Hemorrhage
Clinical picture: fresh blood vomit, bloody / tarry stool, coma, hypovolemic shock (heart rate > 110, systolic BP < 100). Management: ensure airway, breathing, circulation. IV crystalloids or colloids (e.g. hetastarch), monitor / correct electrolytes, gastric lavage, vasoconstrictors, antacids, H2 antagonists, PPI, vasopressin (GI muscle and blood vessel contraction).
Perforation
Sudden acute upper abdominal pain, rebound tenderness, and finally, peritonitis and shock. Symptoms may with time (dangerously misleading). Emergency surgery is needed.
Obstruction
Occurs due to inflammatory edema, spasm or scarring. Clinical picture: postprandial vomiting / bloating, appetite / weight loss, abdominal distension. Management: continuous gastric suction, monitor fluids and electrolyte status, perform saline load test to measure degree of obstruction. Liquids feeding and daily aspirations may be needed.
Post-surgical complications
Dumping syndrome: rapid gastric emptying in 10% of patients after partial gastrectomy. Clinical picture: weakness, dizziness, anxiety, tachycardia, flushing, sweating, abdominal cramps, nausea, vomiting, diarrhea. Occur between 15 and 120 minutes after the meal. Management: eat six small meals, protein and fat and carb. Ingest fluids 1 hr before or after a meal but not with it. Give anticholinergics to delay gastric emptying. Other complications: reflux gastritis, stomal ulceration, diarrhea, malabsorption, early satiety, iron deficiency anemia.
Refractory ulcers
Dyspeptic symptoms after 8 wk therapy. Perform gastroscopy and biopsy to exclude: Crohns disease, TB, lymphoma, carcinoma. Treatment: only PPI offer maximum acid . Eradicate H pylori. D/C NSAID. Perform surgery if all fails.
Maintenance regimens
70% of ulcers recur in a year (90% in 2 years) after healing and therapy d/c. Use long-term maintenance therapy in: concomitant disease, 4 relapses / year, many risk factors (old, male, NSAID, alcohol, smoking, family history, history of complications). H pyloric eradication need for continuous therapy.
Therapy
Antacids
As effective as H2 antagonists. Examples: magnesium, aluminum and calcium salts. Antacids are not widely used for PUD. Continue therapy for only 7 weeks. Typically given 2 hours after meals at bedtime. Effect lasts for 3-4 hours. Mechanism: Neutralize gastric acid gastric pH pepsin activity and mucosal barrier heat and treat ulcer pain. Non-systemic antacids: such as magnesium or aluminum are preferred over systemic antacids (e.g. sodium bicarbonate) to avoid alkalosis. Liquid antacid: buffering capacity than tablets but not as convenient. Antacid mixtures: such as aluminum hydroxide and magnesium hydroxide each drug dose and effect. Side effects are negated (aluminum constipation, magnesium diarrhea). Calcium carbonate: not preferred ( acid rebound, delayed pain relief and ulcer healing, constipation, hypercalcemia). It may produce milk-alkali syndrome esp with milk (hypercalcemia, alkalosis, kidney damage). Acid neutralizing capacity (ANC): number of mEq of a 1 N solution of HCl that can be brought to a pH of 3.5 (99% neutralization) in 15 minutes. For duodenal ulcers, 50 mEq/hr or 125 mEq/day of antacid is needed for neutralization. Precautions: Use calcium and magnesium carefully in renal disease (e.g. elderly). Sodium bicarbonate is CI in hypertension, CHF, renal disease, edema. Use aluminum carefully in patients with dehydration, GI obstruction. Calcium carbonate + alkali (sodium carbonate) + milk = milk-alkali Long term aluminum hydroxide use hypo-phosphatemia, osteomalacia. Aluminum hydroxide is used to treat hyperphosphatemia. Interactions: Generally, take other drugs 30-60 min before antacids. Avoid antacids (polyvalent cations) with tetracycline ( absorption), cipro. May destroy enteric coating leading to premature release in the stomach. Interfere with absorption of: ranitidine, cimetidine, iron, digoxin, phenothiazines, anticholinergics. effect of sucralfate.
H2 receptor antagonists
Preferred in mild-moderate GERD due to lack of effect on GI motility. Mechanism: competitively action of histamine at parietal cell H2 receptors volume and H+ concentration of gastric acid. General SE: nausea, dizziness, renal damage (adjust). Absorption is with antacids (give 1 hr befor e antacids). All available oral or IV. Cimetidine: first drug, gastric acid by 50%. SE: liver damage, hematologic (thrombocytopenia, agranulocytosis, aplastic anemia), weak androgenic (gynecomastia), confusion. Cytochrome P-450 inhibitor metabolism of phenytoin, theophylline, Phenobarbital, lidocaine, warfarin, imipramine, diazepam, propranolol, procainamide. Ranitidine: more potent drug, gastric acid by 70%. Used with bismuth citrate and clarithromycin to eradicate H pylori. Famotidine: most potent, gastric acid by 94% for 10 hr. Nizatidine: newest drug, similar to ranitidine. Oral. DI: absorption of drugs requiring acidic pH (e.g. ketoconazole).
Sucralfate
Non-absorbable disaccharide containing sucrose and aluminum.
Mechanism: adheres to the base of ulcer crater forming a mucosal protectant barrier against acids and bile salts (esp. in duodenal ulcers). Acidic pH is required for polymerization. SE: constipation. Give 1 hr before meals and at bedtime for 6 weeks. Interactions: antacids sucralfate mucosal binding, give 45 min apart. Surcralfate absorption of digoxin, iron, phenytoin, cimetidine, tetracyclines, ciprofloxacin.
GI anticholinergics
Examples: atropine, propantheline. No proven value in ulcer healing Mechanism: basal and stimulated gastric acid and pepsin secretion. Most effective at night in large doses with antacids delay gastric emptying. Or, take 30 min before food ( acid by 40%) SE: dry mouth, blurred vision, urinary retention, constipation, tachycardia CI: gastric ulcer (they prolong gastric emptying), narrow angle glaucoma.
Prostaglandins (misoprostol)
Mechanism: PG E1 analgoue mucus protect gastric mucosa against NSAID damage, bicarbonate, acid. NSAID prostaglandins bicarbonate and mucus secretion damage. Use: QID prevention of NSAID induced gastric ulcer in risk patients. SE: diarrhea, GI pain (take with food). CI: abortifacient, pregnancy category X.
Bismuth compounds
Examples: bismuth subsalicylate (Pepto-Bismol), ranitidine bismuth citrate (RBC), colloidal bismuth subcitrate (not FDA approved). Mechanism: bismuth prevents adhesion of H pylori to gastric mucosa, H pylori growth, release of proteolytic enzymes. Use: most effective in combination with PPI or antibiotics. SE: CNS/neutrotoxicity, dark stool / tongue, headache, diarrhea, rash, salicylism in doses (tinnitus, hyperpyrexia, confusion, tachycardia). Antibiotics for H pylori: metronidazole, tetracycline, clarithromycin, amoxicillin, bismuth subsalicylate, omperazole / lansoprazole. Optimum regimen: bismuth subsalicylate QID + metronidazole QID + tetracycline QID + omperazole QD = 2 wk 90% eradication.
Prokinetic agents
Example: metoclopramide, erythromycin, cisapride (d/c due to incidence of arrhythmia / torsades). Mechanism: ACh release gastric emptying (no effect on acid secretion). SE: diarrhea, GI upset, headache. Interactions: antifungals (ketoconazole, itraconazole, fluconazole, miconazole) cisapride metabolism severe arrhythmia. Rapid gastric emptying can affect absorption of narrow therapeutic drugs. CI: arrhythmia, CHF, ischemic heart, renal failure, respiratory failure.
Surgery
Used in complicated, incapacitating ulcer unresponsive to therapy. Vagotomy: severs a branch of the vagus nerve HCl secretion. Antrectomy: removes the antrum some acid secreting mucosa. Others: gastrectomy, funoplication.
51. Diabetes
Introduction
Definition
1. Dysfunction in metabolism of fat, carbohydrate, protein, insulin 2. Dysfunction of blood vessels and nerves function and structure 2-10% of US population (half undiagnosed)
Classification
General common symptoms: polydipsia, polyuria, dry skin, polyphagia, fatigue, frequent skin / vaginal infections, visual disturbances. 1. Type 1 (Insulin-Dependent, Juvenile-Onset, Ketosis-Prone) Insulin production/secretion is destroyed. Usually in children and adults <30. Prone to ketoacidosis (accumulation of ketone bodies). Dependent on exogenous insulin replacement. 10% of all diabetes. Etiology: a. Genetics: w/ family history. Linked to Human Leukocyte Antigen ( HLA) system. b. Environment: virus (e.g. rubella), toxic chemical triggers genetics / autoimmunity. c. Autoimmunity: anti-insulin and anti-beta-cell antibodies usually present Clinical presentation: abrupt onset, acute presentation. Unintentional weight loss w/ or w/o ketoacidosis. 2. Type 2 (Non-Insulin-Dependent, Adult-Onset) Endogenous insulin is normal, or . May or may not need exogenous insulin. 90% of all diabetes (esp. in the elderly). Adults >30. 80% are also obese. Not prone to ketosis except during stress (infection, surgery, trauma). Etiology: a. Genetics: 90% concordance between monozygotic twins. 15% chance in offspring of diabetics. b. beta cell: insulin. c. Insulin site defect insulin-resistant tissue (insensitivity) Clinical presentation: develops gradually. Evidence of damage to retina, kidneys, peripheral vasculature. 3. Gestational (pregnancy) Glucose intolerance detected during (late) pregnancy (3% of pregnants). Test tolerance 6 wk postpartum. Usually returns to normal. 4. Other types (Secondary Diabetes) Due to disease of pancreas, genetics, endocrinopathies (Cushings), drugs (thiazides, loops, corticostroids, hyperglycemia) 5. Diabetes insipidus: Cause: pituitary disease with production of antidiuretic hormone (ADH) kidney cant conserve water, lithium ( sodium reabsorption). Symptoms: polyuria (20 L / d), severe thirst, polydipsia, watch for dehydration. Treatment: anti-diuretic hormone (vasopressin) analogs desmopressin (oral), lypressin (nasal), maintain fluids / electrolytes. (Desmopressin is also used in Hemophilia A and von Willebrands disease).
Pathophysiology
Normal glucose regulation Insulin:
Structure: endocrine hormone secreted by beta-cells of pancreas. It is a 51-amino acid chain with two polypeptide chains and two inter-chain disulfide bonds. It is derived from proinsulin (86 amino acids). Proinsulin can be used to determine the purity fo insulin products. Mechanism: glucose ATP closes potassium channels membrane depolarization calcium influx fusion of insulin granules insulin release. Insulin and glucose activate N/K ATPase force potassium into the cells hypokalemia. Glucose effects: glucose transport across cell membranes, glucose storage as glycogen in muscles / liver (glycogenesis), glucose formation from glycogen in muscles / liver (glycogenolysis), glucose formation from amino acids (gluconeogenesis) breakdown of fatty acids to ketone bodies (lipolysis) (insulin prevents ketoacidosis absent in type II DM), adipose (fat) tissue formation from triglycerides and fatty acids. incorporation of amino acids into proteins Counter-regulatory hormones: glucagon (from pancreas alpha-cells), epinephrine, norepinephrine, growth hormone, cortisol. Glycogen: carbohydrate consisting of branched chains of glucose units. Principal form of carbohydrate storage, mainly in the liver and muscles. Breaks down easily to glucose when needed.
Abnormal glucose regulation General Insulin insufficiency, resistance hyperglycemia. Liver: glycogenolysis, neoglucogenesis, glycogenesis. Muscle (peripheral tissue): glucose uptake cells use protein as energy source protein breakdown carbohydrates / glucose hyperglycemia. Renal glucose threshold: 180 mg/dl. BG concentration exceeds kidneys glucose reabsorptive capacity glucose excreted into urine (glucosuria) osmotic diuresis dehydration, electrolyte abnormalities coma, death. Diabetic Ketoacidosis (DK) (Type 1) No insulin to break glucose triglycerides breakdown (lipolysis) free fatty acids and glycerol. glycerol liver glucose production hyperglycemia. Free fatty acids acidosis breakdown in the liver ketone bodies kidney excretion ketonuria exceeds kidney excretion limit ketonemia coma, death. A ketone body: acetoacetate converted in the liver to acetone excreted + -3 through the lungs acetone fruity breath. anion gap (Na (Cl + HCO )) Ketone bodies urine detection: add sodium nitroprusside and ammonia purple color. May also occur in severe vomiting or starvation. Initially, the body compensates for acidosis by breathing patterns ( Kussmaul: rapid deep breathing) and by blood buffering systems (bicarbonates, proteins). If Type 2 DM Hyperglycemic hyperosmolar nonketotic syndrome (HHNK), presence of even insulin prevents fat breakdown, ketonemia, ketoacidosis (Ketosis-resistant).
Laboratory findings
Diagnostic criteria: 1. Random BG > 200 mg/dl with classic DM symptoms (polydipsia, polyuria, polyphagia, weight loss). 2. Fasting BG > 125 mg/dl. 3. 2-hour BG > 200 mg/dl during an oral glucose tolerance test (OGTT) using 75 g anhydrous glucose in water. DM predisposition: Impaired fasting glucose (IFG): fasting BG 110-125 mg/dl. Impaired glucose tolerance (IGT): 2-hr OGTT BG 140-200 mg/dl. Gestational diabetes: testing is done at 26 weeks in all women (unless risk: normal weight, no family history, and <25 year). Glucose tolerance: 50 g, after 1 hr if > 140 glucose tolerance: 100 g, 3 hr. Goals of management: euglycemia with no symptoms, prevent acute complications, prevent vascular and neuropathic disease, prevent / treat risk factors ( BP, blood lipids), normal life expectancy and quality of life.
Physical findings: dehydration, serum osmolarity (> 280 mOs), no ketosis / acidosis (hyperglycemic hyperosmolar nonketotic syndrome, HHNK), polyuria, polydipsia, hypotension, tachycardia, palpitations, rapid respiration, nausea, vomiting, abdominal discomfort, CNS function (confusion, coma, seizures, myoclonic jerking). Therapy: insulin, fluid / electrolyte replacement.
Hypoglycemia
Mild hypoglycemia symptoms: adrenergic (tachycardia, palpitations, shakiness), cholinergic (sweating), mild CNS glucopenia ( concentration, dizziness, hunger). Moderate hypoglycemia: CNS effect confusion, motor impairment, no unconsciousness. Severe hypoglycemia: coma, seizure, motor impairment. Pseudo- hypoglycemia: hypoglycemic symptoms perceived (mostly adrenergic) but BG is normal. Hypoglycemia unawareness: no or little symptoms but BG is low. Sweating or neurologic impairment is noticed. Precipitating factors: excess insulin or oral hypoglycermic, delayed or food, exercise, alcohol, drug interaction BG, progesterone in menstruation, new insulin bottle with full potency, gastroparesis (delayed stomach emptying), change in insulin injection site ( absorption if SC near exercising muscle). Treatment of hypoglycemia: if conscious 10-15 g fast acting simple oral carbohydrate (milk, juice, regular soda), 3 g glucose tablet or hard candy, honey, glucose gel. Repeat in 10-15 min if BG is not back to normal. If unconscious IV glucose (10-15% dextrose) or glucagons injection (1 mg IM, SC, or IV).
Long-term complications
Macrovascular
Atherosclerosis: coronary, cerebrovascular, peripheral Peripheral vascular disease: pain, chronic cold feet, insufficient circulation to heal distal lesions gangrene Hypertension: with diabetes cardiovascular disease, stroke, transient ischemic events. Causes acceleration of retinopathy, nephropathy, atherosclerosis. Hyperinsulinemia / insulin resistance diabetic hypertension. Coronary artery disease: autonomic neuropathy Silent myocardial infarction (atypical, no chest pain). Management: daily dose aspirin, ACE inhibitor (for BP), cardio selective beta blocker (for cardiac disease).
Eye (retionopathy)
Consequence of microvascular changes, leading cause of new blindness. Treatment: laser photocoagulation. Nonproliferative (background) retinophathy: retinal microaneurysms, blot hemorrhages, retinal edema, hard exudates, macula edema Preproliferative retinopathy: abnormality of tiny vessels, retinal ischemia, white patches of oxygenstarved retina (soft or cotton-wool spots). Proliferative retinopathy: lack of oxygen weak vessel grow or proliferate (neovascularization) from retinal surface to vitreous cavity. Fragile vessels may bleed into vitreous cavity hemorrhage obscured vision scar tissue and new vessels grow vitreous pull on the retina retinal detachment.
Nephropathy
Most common cause of End Stage Renal Disease (ESRD) microalbuminuria, positive dipstick (clinical) albuminuria, proteinuria / BP, glomerular filtration, creatinine. ACE inhibitors helpful, protein intake, treat UTI. For ESRD fluid / electrolyte restriction, dialysis.
Neuropathy
Peripheral neuropathy: esp. in sensiomotor nervous system. Symptoms first in distal lower extremities then upper extremities (Stocking-glove distribution). Signs: impaired perception of pain / temperature numbness / tingling, impaired balance, proprioception (perception of body parts movement), motor nerve damage muscle weakness / atrophy. Autonomic neuropathy: genitourinary neurogenic bladder, sexual dysfunction. GI gastroparesis, nocturnal diarrhea, fecal incontinence, chronic constipation. Cardiovascular orthostatic hypotension, cardiac denervation.
Therapy
Medical nutrition therapy (MNT)
Carbohydrate counting: 50% of total calories. DM therapy may include pre-meal short acting bolus insulin (lispro, regular, semilente). Otherwise, maintain consistent CHO intake. Fat: limitations on type and amount. Critical for weight loss and treating hyperlipidemia. Target: < 30% of calorie intake and < 300 mg/day cholesterol. Protein: important in end stage renal disease and may delay dialysis. Fiber: bran, beans, fruits, vegetables may help BG and lipids. Alter diet based on stress, illness, exercise, etc. Spaced meal intervals help match hypoglycemic therapy effect.
Physical activity
Careful exercise cell glucose uptake BG Careful if patient has severe retinopathy. Patients with cardiovascular disease or over 45 cardiovascular evaluation and stress test. Aerobic activity: e.g. swimming, walking, running, preferred due to positive effect on BG ( ), cardiovascular, BP, lipids, circulation, weight loss. Anaerobic activity: e.g. weight lifting, should be avoided. Potential negative cardiovascular, BP, retinopathy effects.
Factors insulin requirement: renal failure, weight loss, exercise, nutrient malabsorption, hypopituitarism, adrenal insufficiency. Concentration: U-100 or U-500 for insulin resistance. Source: human, bovine, porcine, synthetic (Lispro insulin, Humalog), or a mixture. Human insulins are made by enzymatic conversion of terminal amino acid of porcine insulin (Novolin, semisynthetic), or by recombinant DNA (Humulin). Human insulin antigenicity. Short-acting: Lispro synthetic, shortest onset and duration. Regular soluble insulin with neutral pH, only clear insulin (IV), only insulin that can be mixed freely. Semilente (prompt insulin zinc suspension) finely divided amorphous prep, use acetate buffer, mix only with other lente, similar duration to Regular, Aspart insulin analogues. Intermediate-acting: NPH (isophane insulin suspension) similar to protamine zinc but with no excess protamine. Lente (insulin zinc suspension) mixture of 70% ultralente crystals and 30% semilente powder. Long-acting: Protamine zinc use phosphate buffer. Ultralente (extended release zinc suspension) large crystalline. Glargine insulin analogue (very long acting). Pre-mixed insulin: 50/50 Regular/NPH, 70/30 Regular/NPH, 75/25 Lispro/Protamine Lispro regular as pre-meal bolus and NPH intermediate for later control of hyperglycemia. Other mixtures can be prepared extemporaneously for tailored ratios. DM Type 1 example: pre-breakfast is 2/3 of total daily dose (TDD) 1:2 short : intermediate. Bedtime is 1/3 of TDD 1:2 like pre-breakfast. Or give pre-supper rapid/short and then bedtime intermediate. DM type 2 example: bedtime only or 2-3 daily injections. Subcutaneous: for routine administration. Absorption of regular insulin is fastest from abdomen > arm > buttock > thigh. Monitor variations in absorption. Randomly rotate injection site to avoid lipohypertropy. If variations avoid random rotation of injection site. Exercise, hot showers, baths, massages blood flow to injection site. Abdomen is least likely to have absorption preferred site for preexercise insulin. Continuous Intravenous (insulin drip): provide Regular insulin for acute hyperglycemia, ketoacidosis, HHNK, or during surgery. Continuous SC infusion (insulin pump): short acting insulin is infused continuously during the day to deliver doses (basal insulin). Bolus dose (determined by algorithms) is delivered by the patient before each meal. Offers tighter glycemic control. Used for diabetics with BG fluctuations, irregular work schedules, lifestyles, or meals. Require frequent SMBG (BG self-monitoring) and training. SE: hypoglycemia (tachycardia, sweating, hunger, convulsions and insulin shock), hypersensitivity, injection site local irritation.
Insulin secretagogues
Drugs (all acidic): Sulfonyrlureas: First gen: chlorpropamide, tolbutamide, acetohexamide, tolazamide, more lipid-soluble, more potent. Second gen: glyburide, glipizide, glimperide. Also repaglinide. Mechanism: block ATP-sensitive potassium channels insulin pancreatic release (primary), and also as sensitizers with time (secondary). Use: Type 2 (useless in type 1, require functioning beta cells). Chlorpropamide: longest duration of action. CI in liver and kidney disease. SE severity and frequency, disulfiram-reaction (also with tolbutamide). Use insulin instead during stressful conditions ( risk of hyperglycemia due to counter-regulatory hormones release). SE: severe / prolonged hypoglycemia (esp. in the elderly, w/ glipizide / glyburide), GI upset, sulfa sensitivity, sun sensitivity, headache, rash, tachycardia, hematologic problems, cholestatic jaundice. CI: allergy to sulfa drugs, pregnancy, lactation. Altered protein binding of sulfonylureas: alcohol, salicylates, NSAIDs, methyldopa, chloramphenicol, MAO-I, clofibrate, probenecid. Therapy failure: due to number of functioning beta cells. Primary: failure to control BG within 4 weeks. Secondary: initial control of BG, but fails to maintain control, due to progression of DM. Repaglinide: less hypoglycemia.
Insulin sensitizers
Drugs: biguanides (metformin, basic drug), thiazolidinediones (rosiglitazone, pioglitazone).
Mechanism: anti-hyperglycermic not hypoglycemic. sensitivity to insulin, (metformin work on liver, hepatic glucose production, gluconeogenesis), (glitazones sensitivity / insulin resistance in muscle and adipose tissue). Thiazolidinediones bind to PPARs. Use: significant insulin resistance. Biguanides SE: fatal lactic acidosis, metallic taste, GI upset, vitamin B12, no hypoglycemia. May be fatal if at risk of lactic acidosis (liver / kidney disease, hypoperfusion, hypoxia, radiography). Phenformin was d/c. Glitazones SE: liver toxicity / failure (monitor), weight gain, edema, GI upset, no hypoglycemia. Troglitazone was d/c due to liver toxicity. CI: liver disease. May resume ovulation in premenopausal women. Highly protein bound (99%).
Alpha-glucosidase inhibitors
Drugs: acarbose (polysaccharide), miglitol (basic monosaccharide) Mechanism: inhibit intestinal enzyme alpha-glucosidase absorption of complex carbohydrates (starch, dextrins, disaccharides). Use only glucose or lactose for correcting hypoglycermia if it occurs. Use: significant post-prandial hyperglycemia. Minimal effect on pre-prandial or fasting BG. Good combination with insulin secretagogues. Take with first bite of meal. SE: GI (diarrhea, abdominal pain, flatulence) due to undigested carbohydrates in the lower GI, no hypoglycemia. CI: GI conditions (inflammatory bowel, colonic ulcer, obstructive bowel, intestinal gas), liver cirrhosis (monitor liver function), pregnancy.
Biosynthesis
Dietary iodine: critical for thyroid hormone synthesis, reduced to inorganic iodide then exracted from plasma by the thyroid by iodide trapping (iodide pump). Organification: oxidation of iodide by peroxidase. Synthesis starts with iodide-tyrosine binding modoiodo then dioiodo-tyrosine.
TT4 hyperthyroidism, and vice versa Serum total triiodothyronine (TT3): Measures total (free and bound) T3. TT3 rise before TT4, useful for early detection. in hyperthyroidism (more than T4), responsible for symptoms. Pregnancy TT3. Resin triiodothyronine (RT3U): Evaluates the binding capacity of TBG. Clarifies whether abnormal T4 is due to thyroid disorder or abnormal protein binding. If abnormal thyroid in the blood RT3U changes in same direction ( in hyperthyroid). If abnormal protein binding RT3U changes in opposite direction ( in hyperthyroid). Serum thyrotropin (TSH) assay: Serum TSH assay: most sensitive test for hypothyroid, but nor reliable in hyperthyroid (TSH is suppressed). Sensitive TSH assay: uses monoclonal antibodies known as immuno-radiometric or immunometric (IMA) method (vs. the older radio-immunoassay). sensitivity, cost, more commonly used to control over treatment of replacement therapy. Free thyroxine (T4) index (FTI): Not a separate test but rather an estimation of free T4 level by a calculation involving serum T4 and RT3U. FTI hyperthyroid or TBG. Strategies for testing Most common and expensive: TT4, RT3U, FTI. Thyroid disease screening for healthy population is not cost effective. Screen only target population (elderly, chronic disease hospitalization, Use FTI and Sensitive TSH for disease diagnosis.
Hypothyroidism
Classification
Primary hypothyroidism: due to gland destruction or dysfunction caused by disease or therapy (radiation, surgery). Secondary hypothyroidism: due to TSH secretion (pituitary disorder). Thyroid gland is normal but not enough TSH stimulation. Tertiary hypothyroidism: TRH (hypothalamus) to stimulate pituitary
Causes
Hashimotos thyroiditis: chronic autoimmune thyroiditis. Treatment of hyperthyroidism: e.g. radioactive iodine, subtotal thyroidectoym, antithyroid drugs. Goiter: enlargement of the thyroid gland. Endemic goiter: due to inadequate dietary iodine (malnutrition). Sporadic goiter: due to foods or drugs containing progoitrin (inactive hydrolysis active goitrin) oxidation of iodine to iodide. Goitrogenic drugs: propylthiouracil (PTU), iodides, cobalt, lithium, phenylbutazone. Other causes: thyroiditis, thyroid cancer. Surgical excision
Symptoms: coma, hypothermia, respiratory rate failure, hypometabolism fluid / electrolyte retention fluid retention, hyponatremia, heart rate / contractility, heart output. Treatment: rapid restoration of T3 and T4 to normal levels. IV bolus levothyroxine, oral liothyronine, then oral levothyroxine.
Drugs
Desiccated thyroid preparations: not commonly used anymore. Different preparations are not bioequivalent (varying amounts of actives depending on source (bovine, ovine, porcine). Fixed ratio (liotrix) preparations: standard ratio of T4/T3. T3 is, however, unnecessary (T4 converts to T3) cause SE tremor, headache, palpitations, diarrhea. Levothyroxine: agent of choice, predictable results, no T3. Individual variable response to different preparations care if to switch. Use dose for elderly or chronically ill patients. Results start after 2 wk, full response after 4-5 months (TSH levels to normal levels).
Precautions
Careful in the elderly and in case of cardiac disease. S tart with doses. Watch for cardiac complications (palpitations, arrhythmia, angina). Monitor thyroid levels (T4, RT3U, FTI, sensitive TSH). Long term levothyroxine therapy can cause thyrotoxicosis. Accelerated bone loss due to over treatment nontraumatic fracture. CI: cholestyramine (bile acid sequestrant) thyroxine bioavailability. Separate drug by 6 hours.
Hyperthyroidism / thyrotoxicosis
Graves disease (diffuse toxic goiter)
Most common form. Occurs usually in young women. Autoimmune disease, antibodies (long-acting thyroid stimulators, LATS) bind to and activate TSH receptors (does not actually increase TSH itself). Symptoms: enlarged goiter, exophthalmos, stare, nervousness, irritability, anxiety, insomnia, heat intolerance, sweating, appetite, weight, muscle tremor / weakness, tachycardia, palpitations, diarrhea. Signs:
Other forms
Jodbasedow phenomenon: hyperthyroid due to iodine ingestion or amiodarone. Factitious hyperthyroidism: due to abusive ingestion of thyroid replacement drugs to lose weight.
Drugs
Beta blockers propranolol Propranolol peripheral symptoms (tachycardia, sweating, tremor, nervousness). It also peripheral T4T3 conversion (deiodonation). Antithyroid drugs Examples: propylthiouracil (PTU), methimazole. Mechanism: interferes with thyroid hormone synthesis by iodide oxidation. PTU peripheral T4 T3. Dosing: initial dose (2 mo), maintenance dose (12 mo), gradual withdrawal (2 mo). Restart therapy if signs of hyperthyroidism appear. Monitor serum thyroid, FTI and goiter size.
SE: skin rash, urticaria, pruritus, hair loss, skin piementation, drowsiness, myalgia, arthralgia. Severe SE: blood (agranulocytosis, granulocytopenia, thrombocytopenia), monitor blood count. Radioactive iodine (RAI) Mechanism: thyroid gland picks up the radioactive element iodine-131 as it would regular iodine. Radioactivity destroys cells. Advantages: cure rate (100%), avoid surgical risks, cost Disadvantages: risk of delayed hypothyroidism, delayed effect. SE: only for women past childbearing years. Response is hard to gauge (too much, too little). Subtotal thyroidectomy Partial removal of the thyroid gland. Last resort. Advantages: success rate, rapid cure. SE: thyroid storm, permanent hypothyroidism.
Complications
Hypothyroidism: may follow Graves disease. Thyroid storm (thyrotoxic crisis): is a sudden exacerbation of hyperthyroidism caused by rapid release (leakage) of thyroid hormone ( T4) fever, tachycardia, restlessness, tremor, hyper-meabolism dehydration, shock, death if not treated rapidly. Precipitating factors: thyroid trauma, surgery, radiation, infection, sudden d/c of antithyroid therapy. Treatment: PTU, methimazole, proproanolol, potassium iodide ( intrathyroidal iodine intake), supportive therapy (rehydration, cooling, AB, rest, sedation).
Incidence
Second leading cause of death in the US. Affects 30% of all people at some point in life. Some forms of cancer are curable if detected / treated early.
Etiology
Viruses: Epstein-Barr virus, hepatitis B, human papilloma viruses. Environmental / occupational exposures: ionizing / UV radiation, chemicals (benzene, asbestos, vinyl chloride). Life-style: fat, fiber diet, ethanol, tobacco. Medications: alkylating agents, immunosuppressants. Genetics: inherited mutations, cancer-causing genes (oncogenes).
Detection / diagnosis
Warning signs: CAUTION. Change in bowel / bladder habits, A sore that does not heal, Unusual bleeding / discharge, Tissue thickening or lumps (e.g. breast), Indigestion of difficulty swallowing, Obvious change in a wart or mole, Nagging cough or hoarseness. Guidelines for screening: for asymptomatic people mammography (breast cancer), fecal occult blood test (colon cancer), Pap smears (cervical cancer). Tumor markers: biochemical indicators of the presence of neoplastic proliferation in serum, plasma, other body fluids. Not definitive. Include: prostate specific antigen (PSA), carcinoembryonic antigen (CEA), alpha fetoprotein (AFP). Tumor biopsy: definitive test for cancer cells is pathology of a biopsy.
Imaging studies: x-ray, computerized tomography scans, MRI, positive emission tomography. Lab tests: complete blood count, blood chemistries.
Staging
It is the categorizing of patients according to extent of the disease. Used to determine prognosis. Two system are used for neoplasm staging. TNM: T = tumor size (0-4), N = regional lymph node spread (0-3), M = presence of absence of distant metastases (0-1). Example: T2N1M0. AJC: by the American Joint Committee on staging. Scale: 0-IV.
Survival
Depends on tumor size, disease extent, treatment received. 60% survive more than 5 years, but not all survivors are cured. Complete response or remission when no evidence of disease after treatment. Slow growing tumors 10-15 disease free years.
Chemotherapy
Therapy objectives
Cure: sought with aggressive therapy for long time to eradicate all disease. Example for leukemia: remission induction, attempt maximal cell kill and therapy consolidation to eradicate all clinically detectable disease and get tumor cell count 1000. Palliation: goal is to control symptoms when complete eradication of tumor is unlikely or if patient refuses aggressive therapy. Adjuvant: given after more definitive therapy (e.g. surgery) to eliminate any remaining disease. Neoadjuvant: goal is to tumor burden before surgery or radiation.
Dosing
May be bases on body weight, BSA or AUC. BSA is preferred (correlates with cardiac output which determines renal / hepatic blood flow / elimination). Adjust dose for liver or kidney dysfunction. Dosing is usually given as short courses in cycles.
Combination chemotherapy
To overcome or prevent resistance, achieve cytotoxicity to resting and dividing cells, enhance biochemical effect, rescue normal cells. Acronyms are often used to indicate certain combinations.
Administration
IV is the most common Inrathecal: for methotrexate, hydrocortisone, cytarabine, thiotepa.
Response to chemotherapy
Does not always correlate with survival. Complete response: disappearance of all disease (clinical, gross, microscopic). Partial response: > 50 reduction in tumor size for a period of time. Response rate: defined as complete response + partial response. Progression or no response: > 25 increase in tumor size or appearance of new lesions.
Antitumor antibiotics
Most come from Streptomyces Mechanism: alkylation (mitomycin) or intercalation. Intercalation: drug slides between DNA base pairs and DNA synthesis. Anthracyclines: daunorubicin (daunomycin), doxorubicin (adriamycin, hydroxydaunorubicin), epirubicin, idarubicin. Anthracendiones: mitoxantrone Others: bleomycin, dactinomycin, mitomycin, plicamycin (mithramycin).
Antimetablites
Structural analogs of naturally occurring substrates for biochemical reactions. Mechanism: false substitution in production of nucleic acid DNA synthesis. Adenosine analogs: cladribine, fluudarabine, pentostatin (deoxycoformycin). Folic acid analogs (folate antagonists): methotrexate, trimetrexate, raltitrexed. Purine analogs (purine antagonists): mercaptopurine, thioguanine Pyrimidine analogs (pyrimidine antagonists): fluorouracil, capecitabine, cytarabine, gemcitabine.
Plant alkaloids
Vinca prevent formation of the mitotic spindle arrest cell division. Examples: vinblastine, vincristine, vindesine, vinorelbine. Camptothecins inhibit topoisomerase I. Examples: irinotecan, topotecan. Podophyllotoxins inhibit topoisomerase II. Examples: etoposide, teniposide. Taxanes microtubule assembly / stabilization cell division. Examples: taxol (paclitaxel), taxotere (docetaxel).
Hormones
Androgens: testosterone, fluoxymesterone Antiandrogens: bicalutamide, flutamide, nilutammide. Antiestrogens: tamoxifen, toremifene. Aromatase inhibitors: letrozole, anastrozole, exemestane, aminoglutethimide. Corticosteroids: prednisone, dexamthasone Estrogens: ethinyl estradiol, diethylstilbestrol. Estrogen/nitrogen mustard: estramustine Progestins: medroxyprogesterone, megestrol Luteinizing hormone releasing hormone analogs: leuprolide, goserelin
Asparaginase
Mechanism: enzyme that causes the degradation of essential AA asparagine to aspartic acid and ammonia. Normal cells can synthesize asparagine but tumor cells can not.
Dermatological
Alopecia: partial or complete, can not be prevented. Local necrosis: results from extravasation during administration of vesicant drugs immediate pain / burning + possible delayed reaction tissue damage, necrosis, ulceration require plastic surgery. Treatment: cold for all drugs except vinca and taxanes (use heat). Skin changes: dryness, sun sensitivity (methotrexate, fluorouracil).
GI toxicity
Nausea and vomiting Most distressing SE from the patients viewpoint. Acute, delayed or anticipatory. Antiemetics are used for prophylaxis. Vomiting dehydration, electrolyte imbalance, esophageal tears d/c therapy. Stomatitis Common with methotrexate, fluorouracil (same as skin changes) General inflammation of the oral mucosa or other areas of the GI with rapid turnover of cells. Symptoms: erythema, pain, mouth dryness, lip tingling / burning, ulceration, bleeding infection, inability to eat. Time course: starts in 1 week, resolve in 2 weeks. Other SE: fluorouracil diarrhea, vincristine constipation.
Pulmonary
Irreversible and may be fatal. Especially with bleomycin, mitomycin. Symptoms: breath shortness, unproductive cough.
Cardiac
Acute: transient ECG abnormalities. Not important. Chronic: irreversible CHF due to drugs or radiation. Dexrazoxane: cardioprotective (use with doxorubicin).
Neurotoxicity
Due to intrathecal or systemic therapy. Autonomic / peripheral neuropathy: due to vincristine. Vincristine is fatal if given intrathecally. Peripheral neuropathy / ototoxicity: due to cisplatin. Arachnoiditis: due to intrathecal methotrexate, cytarabine.
Hemorrhagic cystitis
Bladder toxicity due to cyclophosphamide and ifosfamide. Acrolein: metabolite of these drugs chemical irritation of bladder mucosa bleeding. Prevention: aggressive hydration with frequent urination, mesna (binds to acroltein prevents it from contacting bladder mucosa).
Other
Hypersensitivity: may be life threatening (anaphylaxis). Chills / fever: especially with bleomycin. Hepatoxocity: liver function tests, jaundice, hepatitis. Nephrotoxicity: serum creatinine, BUN, electrolyte imbalance. Use amifostine to protect the kidney if using cisplatin. Secondary malignancies: such as leukemia, solid tumors, lymphoma. Female infertility: may be temporary or permanent .
Analgesics
Non narcotic analgesics
General uses: antipyretic, anti-inflammatory (except acetaminophen), analgesic ceiling effect, no tolerance, no dependence. OTC: aspirin, acetaminophen, ibuprofen, ketoprofen, naproxen (low doses). General SE 1. Gastro-intestinal: Due to PG inhibition. With all except acetaminophen, COX-II inhibitors, choline magnesium trisalicylate, etanercept. Symptoms: dyspepsia, ulceration, bleeding, perforation. Especially in the elderly, ulcers, smokers, alcoholics. Avoid by combo therapy with GI protectants (PPI, misoprostol, H2-antagonists, antacids, sucralfate) or enteric coating (aspirin). 2. Hematologic: Exceptions: acetaminophen, choline mg trisalicylate, etanercept. Inhibit platelet aggregation by reversibly inhibiting PG synthase. Aspirin is an irreversible inhibitor. Contraindicated with anticoagulants (warfarin, heparin, etc) 3. Renal:
PG inhibition, interstitial nephritis, impaired renin secretion, tubular water/Na reabsorption reversible abrupt oliguria Salicylates Chemistry: derivatives of salicylic acid (from Willow bark). Weak acids with excretion by pH. Aspirin is acetyl salicylic acid, hydrolyses easily, unstable in water, moisture. Other salicylates: diflunisal, methyl salicylate (topical, wintergreen oil), salsalate, mesalamine, olsalazine, sulphasalazine, sodium thiosalicylate (injection), choline salicylate (oral liquid). Pharmacology: cyclooxygenase (COXI/II) local PG analgesic, antipyretic, anti-inflammatory. Aspirin is the only salicylate that COX irreversibly by covalent acetylation. Also, platelet COX thromboxane A2 formation platelet aggregation / thrombus formation. Indications: analgesics (skeletal muscle pain, headache, neuralgia, myalgia, spasmodic dysmenorrhea), anti-inflammatory (arthritis, rheumatic fever), antipyretic (avoid in children with viral infection Ryes syndrome), prophylaxis of MI. Mesalamine, sulphasalazine, olsalazine inflammation in inflammatory bowel disease, Crohns disease. Methyl salicylate topical counter irritant. SE: GI upset (nausea, vomiting, discomfort, irritation, ulceration, hemorrhage), bleeding, delayed labor, depth of respiration, hyperglycemia, glycosuria. Low dose (2 g) urate excretion ( blood level). High dose (5 g) opposite. Toxicity: salicylism (tinnitus). Oral methyl salicylate can be fatal. Sulphasalazine male infertility. Acute hypersensitivity (asthma, rhinitis, urticaria, shock, etc). May have cross-sensitivity to other NSAID DI: Oral anticoagulants (due to platelet inhibition and gastric mucosal damage bleeding). Methotrexate: toxicity with salicylates by blocking methotrexate renal tubular secretion. NSAIDs Examples: (x-profen) ibuprofen, ketoprofen, fenoprofen, flurbiprofen, naproxen (sodium), indomethacin, piroxicam, diclofenac, ketorolac (oral, IM), etodolac, oxyprazocin, tolmetin, sulindac, meclofenamate, mefanemic acid, nabumetone. COXII inhibitors: celecoxib, rofecoxib, valdecoxib. Chemistry: Many are acid derivatives. Most are from propionic (x-en) or acetic acid. Others: fentamates, oxicams or anthanilic acid derivatives. COX-II inhibitors pyrazole derivatives. Pharmacology: COX-I produces PG cytoprotective of stomach lining. COX-II produces PG for pain / inflammation. NSAIDs: COXI/II local PG synthesis. COX-II inhibitors: COXII only. Indications: NSAIDs: mild to moderate pain, rheumatoid arthritis, osteoarthritis, gout, additive analgesia with narcotics. COX-II inhibitors: rheumatoid arthritis and osteoarthritis. Ketorolac IM: for moderate to severe pain (strongest NSAID for analgesia) when narcotic are undesirable (addicts, respiratory depression, sedation). Indomethacin: strongest NSAIDS for inflammation, CNS SE. Use for ductus arterisous in premature infants. SE: NSAIDs: GI upset (dyspepsia, mucosal erosion), CNS depression / drowsiness, platelet function, skin rash, kidney damage. COX-II inhibitors: kidney damage, GI upset. DI: NSAIDs effect of diuretics (due to renal perfusion). COXII inhibitors are CI in allergy to sulfonamides, aspirin, NSAIDs p-Aminophenols Acetaminophen is the prototype (APAP, acetyl para-amino phenol). Also, phenacetin. Mechanism: central PG analgesic, antipyretic. No peripheral PG blocking no effect on inflammation, platelets. Use: alternative antipyretic, analgesic to salicylate. Unlike aspirin, safe as antipyretic for children with viral infections. SE: at normal doses (skin rash). Acute overdose liver failure. Antidote: N-acetyl cysteine. CI: alcoholism. Pyrazolones Chemistry: prototype is phenylbutazone, its metabolite is oxyphenbutazone. Also sulfinpyrazone. Mechanism: PG synthesis, stabilize lysosomal membrane analgesic, antipyretic, anti-inflammatory, uricosuric. Sulfinpyrazone only uricosuric hyperuricemia in gout.
Use: (oxy)phenylbutazone short term treatment of rheumatoid arthritis and gout (not first choice). SE: SE. blood dyscrasias (agranulocytosis, thrombocytopenia, anemias), GI uspet, ulceration, kidney damage, hyperglycemia, skin rash, CNS (drowsiness, headache).
Nausea / vomiting: due to central stimulation of chemoreceptor trigger zone, esp. in parenteral dosing for acute pain. May need anti-emetic (hydroxyzine, prochlorperazine), but may sedation. Sedation: dose-related and with other sedatives (BZD, anti-emetics). Tolerance develops if chronically used. May need CNS stimulant (methylphenidate, dextroamphetamine). Different from physiological sleep (pain is controlled patient rests). Anticholinergic: dry mouth, urinary retention. Hypersensitivity: not true allergy. Itching or wheel at injection site due to histamine release, esp. with intrathecal or epidural. Meperidine CNS excitation: seizure-like, esp. in renal failure patients. Due to accumulation of normeperidine metabolite. Tolerance: to analgesic, sedative and euphoric effects. Combo with NSAID may help overcome this problem. Other SE: miosis, euphoria, confusion / hallucinations, coma, orthostatic hypotension, arrhythmias, histamine release (itching, vasodilation BP, bronchoconstriction). Dependence: Withdrawal symptoms: anxiety, irritability, insomnia, chills, salivation, rhinorrhea, diaphoresis, nausea, vomiting, GI cramping, diarrhea, piloerection. Long t 1/2 less intense / delayed withdrawal. Reduce acute withdrawal by using antagonist (naloxone) or agonist-antagonist (pentazocine). Drug interactions: additive CNS depression (alcohol, anesthetics, antidepressants, antihistamines, barbiturates, benzodiazepines, phenothiazines). Meperidine with MAO inhibitors hypertension, excitation, rigidity.
Tramadol
Oral, centrally acting, non-controlled, analgesic with weak opiate (mu) activity for moderate to severe pain. Chemically unrelated to opioids. Mechanism: bind to opiate receptors norepinephrine, serotonin reuptake. Naloxone is a partial antagonist. SE: GI (nausea, constipation, dry mouth), CNS (dizziness, drowsiness, headache), respiratory depression, histamine release. DI: sedation with alcohol and hypnotics. Inhibits MAO avoid with MAO inhibitors ( seizures)
Miscellaneous agents
Glucosamine sulfate and chondroitin sulfate For degenerative joint disease (arthritis) Glucosamine: substrate and stimulant for biosynthesis of hyalouronic acid and glucosaminoglycans forming proteoglycans in structural matrix of joints. SE: GI, drowsiness, headache, rash. Chondroitin: substrate for formation of healthy joint matrix Analgesic adjuncts Other drugs affect non-opiate pain pathways may help with certain types of pain (e.g. neurogenic / neurologic), or to SE Examples: tricyclic antidepressants, anticonvulsants, BZD, neuroleptics, corticosteroids, antihistamines, amphetamines. Non-pharmacological pain management Include Cognitive Behavioral Interventions (education, instruction, relaxation, biofeedback, hypnosis), and Physical methods (acupuncture, physical therapy, compression gloves, orthotic devices, heat / cold, massage, immobilization, exercise, rest, transcutaneous electrical nerve stimulation (TENS)
b. Marasmus:
Chornic state (over months or years) that result from deficiency in the total calorie intake depletion of fat stores and skeletal proteins to meet metabolic needs. Visceral protein is preserved (normal serum albumin, prealbumin, transferrin). Immune competence, wound healing and ability to handle short term stress are preserved Aggressive nutritional repletion can result in metabolic distrubances (e.g. hypokalemia, hypophosphatemia)
c. Kwashiorkor
Acute pricess (within weeks) due to inadequate protein intake Visceral protein depletion, impaired immune function Hypermetaboism (e.g. trauma, infection, surgery) + protein deprivation kwashiorkor malnutrion, hypoalbuminemia, edema Aggressive nutritional protein repletion is warranted
B. Metabolic requirments:
1. Energy requirments Determined as nonprotein calories (NPC). Can be measued by:
a. Indirect calorimetry or Measured Energy Expendure (MEE) Most accurate. Directly measures O2 consumption and CO2 production. Energy requirment is directly related to oxygen consumption. Respiratory quotient (RQ) = CO2 produced / O2 consumed Oxidation of nutrients: carobohyrates RQ = 1.0, fat RQ = 0.7, Lipogenesis: conversion of excess carbohyrate calories to fat, produces more CO2 than oxidation. b. Estimated energy expendure (EEE) Requires calculation of basal energy expendure (BEE) from Harris-Benedict equation. BEE is then multiplies by stress and substrate utilization factors. c. Simple nomogram Based on patient weight, least accurate. Range from 2535 Kcal/kg/day depending on degree of stress. 2. Protein (nitrogen) requirments a. Nitrogen balance techniques 16% of protein is comprised of nitrogen Nitrogen balance = 24hr nitrogen intake 24hr nitrogen output Nitrogen output = urine urea nitrogen + nonurea urine nitrogen (ammonia, creatinine) + nonurine nitrogen loss (skin/feces) Positive nitrogen balance of 3-6 g is the goal (not for the renally impaired) b. Nomogram method: estimates protein needs based on lean body weight (1.5-2.0 g protein/kg/day) c. Nonprotein calorie to nitrogen (NPC:N) ratio: normally 125-150:1 3. Essential fatty acids (EFAs): EFAs are polyunsaturated fatty acids not synthesized by humans. Linoleic acid: principal EFA. Its omega-6 polyunsaturated fatty acid. Linoleic acid deficiency diarrhea, dermatitis, hair loss Prevent EFAs deficiency by giving ~5% of patients calorie intake as linoleic acid from lipid emulstion. 4. Vitamins: Fat soluble: A, D, E, K; Water soluble: B, C Vitamin A: essential for vision, growth, reproduction. IV form binds to plastic and glass. Vitamin D: regulate calcium / phosphorous homeostasis together with calcitonin and parathormone. Vitamin E: antioxidant, oxidation of free unsaturated fatty acids. Need to Vitamin E in diets in unsaturated fatty acids. Vitamin K: critical for synthesis of clotting factors. Vitamin B1 (thiamine): coenzyme in phosphogluconate, structural component of nervous system membranes. Deficiency acute pernicious beriberi. Prolonged deficiency Wernickes encephalopathy. Vitamin B2 (riboflavin): coenzyme in oxidative phosphorylation. No intracellular stores maintained. Vitamin B3 (niacin): conenzyme in oxidative phosphorylation. Deficiency pellagra. Vitamin B5 (pantothenic acid): functional form is coenzyme A, essential for all acylation reactions. Vitamin B6 (pyridoxine): coenzyme in enzymatic reactions. Deficiency when taking isonizid, penicillamine, cycloserine. Vitamin B7 (biotin): synthesized by intestinal floar. Involved in carboxylation reactions. Vitamin B9 (folic acid): folate cofactors are needed for purien and pyrimidine (DNA) synthesis. Deficiency in B12 deficiency in (B9) folate megaloblastic anemia. Deficiency during pregnancy neural tube fetal defects. Vitamin B12 (cyanocobalamin): large stores deficiency develops in years. Deficiency: megaloblastic (pernicious) anemia, peripheral neuropathy (needed for myelin synthesis). 5. Trace minerals Iron: necessary for hemoglobin and myoglobin production, enzymatic reactions (cofactor). Deficiency: hypochromic, microcytic anemia, immune deficiency. Zinc: necessary for RNA, DNA synthesis and enzymatic reactions (cofactor). Deficiency: imparied wound healing, growth retardation, hair loss, anorexia. risk of deficiency in long-term steroid therapy, malabsorption, surgery. Copper: necessary for heme synthesis, electron transport, wound healing. Deficiency: anemia, leukopenia, neutropenia.
Manganese: involved in protein synthesis Selenium: for antioxidant reactions. Deficiency: muscle pain, cardiomyopathy. Iodine: component of thyroid hormones. Deficiency: goiter Chromium: critical for glucose use, insluin effect. Deficiency: hyperglycemia, glucose intolerance. Molybdenum: essential to xanthine oxidase
100. The Patient Behavioral Deterimants 102. Drug Education 103. Patient compliance
Definition: extent to which an individuals behavior coincides with medical or health advice. Noncompliance can be intentional or unintentional. About 50% of the population is noncompliant with drug therapy in some way. Causes in the elderly: complicated drug regimen, inability to read labels, difficulty opening lids, etc. Noncompliance can affect and bias the results of clinical studies.
Types of noncompliance
Not filling Rx: because they do not feel they need or want the Rx. Example: an infection with Tylenol is feeling better and improving. May be because of cost. Omission of doses: common for drug that are taken frequently for long time. Wrong dose: amount of does or frequency of administration is incorrect. Incorrect administration: for example, not using the right technique with aerosols, or wrong route of administration. Wrong time: for example, drug taken at the wrong time in relationship to meals. Drugs such as tetracycline, fluoroquinolones, erythromycin should be taken on empty stomach. Diuretic should be taken in the morning. Premature d/c: common with antibiotics (symptoms subside) or chronic drugs such as for BP (asymptomatic). Storage: improper storage and improper disposal of unused drugs.
Consequences of noncompliance
Over and under utilization have major economic impact. Always, the benefits from compliance outweigh the costs of compliance enhancing programs. Overutilization: may cause toxicity. Examples: double dose to make up for missed dose, if one pill is good then more must be even better. Noncompliance is one of the most commonly missed diagnoses (e.g. poorly controlled BP). Consequences of noncompliance are not always negative. Some patients are intelligent noncompliant where they alter the dose based on SE emergence while treatment goals is still achieved.
Detection of noncompliance
Diagnosis of the problem is a key. Behavior may change with time. Ideal detection takes place at the time and place of taking the medication.
Indirect measures:
Self-reports and interview: simplest, but overestimates compliance. Most people have trouble remembering to take their medicine. Do you have trouble remembering to take yours? Pill count: commonly used in clinical studies. Pill dumping is a common problem (study participants try to deceive physicians). Overestimate compliance. Change of weight of MDI can be used. Achievement of treatment goal: examples: normal BP, BG, intraocular pressure. However, patients may load-up on medication or use other regimens (diet) before doctor visit. This is called toothbrush effect (people toothbrush before dentist visit).
Computerized compliance monitors: most reliable indirect method. Started with electronic eye-drop dispensers. A microprocessor is located in the cap of the container. Time and date are recorded every time the patient removes the cap. Very useful in clinical studies. Refill rate: commonly used in community pharmacy settings.
Direct measured:
Direct methods are more reliable. Use of at least 2 methods is recommended. Biological markers and tracer compounds: indicate patient compliance over extended period. Example: glycosylated hemoglobin assesses BG control over the preceding 3-months. Tracer compounds: small amounts of agents such as Phenobarbital or digoxin (long half life, indicate compliance for past weeks) are added to drugs and measured in biological fluids. Drug concentration in biological fluids: limited usefulness due to variability between individuals, does not indicate the timing of the dose, can be fooled by loading-up prior to biological fluid sampling.
Therapeutic regimen
Multiple drug therapy: number of drugs noncompliance (e.g. in geriatrics). The similarity in appearance of drugs may lead to confusion. Combination drugs may help but therapy should start with individual drugs and then switched to the combo when optimum dose is reached. Frequency of administration: may cause interruption of normal routine or work schedule inconvenience, embarrassment, forget. Very critical factor in compliance. However, patient may be skeptical about the effective of a QD drug. Duration of therapy: rate of noncompliance as duration . Adverse effects: change dosage or use alternative drugs if possible. Big problem when the medication makes the patient feel worse than before (e.g., BP drugs). Sexual dysfunction is common cause (e.g.
with antipsychotics, antihypertensives). Just communicating potential SE may cause the patient not to take the drug. Asymptomatic conditions: includes lack of symptoms before the drug, lack of appearance of symptoms if drug is d/c, disappearance of symptoms (antibiotics). Cost: cost Rx not filled, dose is taken, frequency, prematurely d/c. Administration: for example, incorrect measure of liquid medications, MDI use, oral antibiotic drops for ear infection instilled in the ear, using suppository by the oral route. Taste: common for oral liquid in children (e.g. liquid KCl).
Patient/pharmacist interaction
Psychological support should be provided in a compassionate manner. Patients are compliance with a physician they know and respect. Not appreciating importance of therapy: if therapy does not meet their own or taught expectations noncompliance. Poor understanding of instructions: as directed should be avoided on label. every 8 hours is more specific than three times a day. Auxiliary instructions are also key. Example: apply one nitroglycerin patch a day, patient got confused and added a new patch without removing the old ones.
Improving compliance
Identification of risk factors
All patients should be viewed as potential noncomlpiers. Evaluate the probability of being noncompliant based on the risk factors.
Patient education
Effective communication is the key for compliance. Patients should be asked to repeat the instructions to show understanding. Key points: name of medication, action, how much to take, when, for how long, food interactions, possible SE, what to do about SE, information sheet. Oral communication / counseling: more important than written, as it gives patient a chance to interact and ask questions. Ensure privacy and distractions. Separate consultation area is ideal. Call the patient if possible if face to face is not possible. Written communication: important is a future reference for the patient as he is not expected to remember all details. Written info compliance only for short term therapy (e.g. antibiotics). Audiovisual materials: very useful in certain situations (e.g. insulin, sumatriptan, MDI). Controlled therapy: it is recommended that patients start self-medication before hospital discharge to transition them from complete dependence in the hospital to complete independence at home. Special compliance programs: example: behavioral program for schizophrenics. Training include learning on obtaining information about drug benefits, correct self-administration and evaluation of effects, identify SE, talking about issue with professionals. Programs may be useful also for sight or hearing impaired patients.
Patient motivation
Good knowledge about the illness and medication does not necessarily translate to compliance. Patients need to be motivated not only educated. Information must be presented in a manner that is not coercive, threatening, or demeaning. Use special packaging or reminder systems if possible. A contract approach may be useful with some patients where agreement is reached on specific actions.
Compliance aids
Labeling / auxiliary: must be clear, accurate and specific Calendars / Reminder charts: helps the patient understand which medication to take and when to take it. Special containers / caps: for example, system with four compartments for different time periods (morning, noon, evening, bedtime) for each day of the week. Special caps can display the time of the day when the last dose was taken. It flashes / beeps when it is time for the next dose. Compliance packaging: defined as pre-packaged unit that provides one treatment cycle of the medication. Usually based on blister packages. A good example is special packaging for birth control pills. Another example: prednisone decreasing dose regimen. Child-proof caps may be a problem for the elderly or patients with arthritis. Dosage forms: for example ER, XR and transdermal patches.
Monitoring therapy
Self monitoring: by the patient of the treatment regimen, response parameters. Pharmacist monitoring: based on inadequate frequency of refills, follow up by phone or mail reminders. Automatic phone call reminder systems have been used. Brown bag program: elderly pull all medications in a bag and take them to a professional for review. Directly observed treatment: watch patient swallow drug (e.g. in TB).
112. Pharmacoeconomics
Innovative roles for pharmacists: home IV therapy, drug level monitoring, parenteral nutrition management, self-care counseling. Pharmacy services may provide positive outcomes by morbidity, therapeutic control, cost of treatment by using efficient therapy, # of physician visits, rate of drug related hospitalization, incidence and intensity of SE. Extra years of life for a patient population can be converted to dollars for society. Economic methods Technique Inputs Outputs Classical operations analysis Units (e.g. pharmacy hrs) Units (e.g. patients monitored) Cost effectiveness analysis Dollars Natural units Cost benefit analysis Dollars Dollars Cost utility analysis Dollars Utiles/preferences Cost minimization analysis Dollars Assumed equal
Benefits
Benefits: defined as the in costs realized due to program implementation. Can be direct, indirect, or intangible. Direct benefits: savings on direct costs in medical care. Easy to measure. Indirect benefits: savings on indirect costs in the medical care. Difficult to measure. Its avoidance of earnings and productivity losses which would have been incurred without the health program. Intangible benefits: difficult, if not impossible, to measure. Intangible costs are psychological (pain, suffering and grief).
Discount rates
Discount rate is the conversion of dollar amount to present values through the use of interest rate. discount rate: favors projects with benefits occurring in distant future. discount rate: favors projects with costs occurring in distant future.
Commonly used discount rate is the yield rate on long term gov bonds. Mathematical models are used to calculate benefit/cost ratio. Net Present Value (NPV): a new model for calculating benefits-costs. Very popular and currently recommended by many economists. Rate of Return on Investment: calculates the interest rate from an initial program investment over a potential stream of benefits over time.
Economic perspectives
A pharmacy service with positive benefit/cost ratio may be good for the society as a whole but not to every segment of the society. Example: drug regiment that # of patient days in acute care is good for the society but may not be good for the hospital that depends on patient stays for revenue. Always consider who pays the costs and who receives the benefits.
Liabilities: debt owned by the business to creditors. It arises when business borrows cash (e.g. bank loan) or purchases goods or services on credit. Examples: accounts payable, notes payable. Owner equity (Net Worth): claim of the owners to the assets of the business after all creditors have been paid. It when owners make investments in business or when revenue is earned. It when expenses are paid. Examples: contributed capital, sales revenue, service revenue, expense accounts. Expenses: not a liability because they are used up resources that require the immediate payment of cash for the amount in full, otherwise liability. Prepaid expenses are assets because theyre resources that have not yet been used up. Income = revenues expenses. Cost of Goods on Hand: on last day of accounting period physical inventory to determine cost of inventory not sold. No physical inventory is needed if perpetual inventory is kept by computer systems. Fixed assets: tangible, long-lived resources used in business operation, e.g. building, machinery, fixtures, equipment, etc. Current assets: resources owned by the business which are expected to be realized in cash, sold or consumed in one year, e.g. account receivable, inventory, etc. Depreciation: wear and tear that occurs on fixed assets calculated as an expense. Most fixed assets, except land, are depreciated. Contra (offset) accounts: reside directly below the fixed asset account to which they pertain.
Income statement
Summary of operations, income earned during accounting period. Constructed using revenue and expense account balances. Revenue: sales of good and services Cost of goods sold: such as inventory and transportation expenses. Gross margin = revenue cost of goods sold. Net profit (income) = revenue all expenses. Net income = net profit income tax
Balance sheet
Presents the financial position of the business at a certain point in time Constructed using all asset account, liability accounts, OE accounts Retained earnings: link income statement and balance sheet.
Cycle stock: inventory kept on hand to fulfill orders Buffer/safety stock: inventory for case of supply/demand fluctuations. Anticipatory/speculative stock: inventory for expected in demand
Steps of purchasing
Cost of goods sold (COGS): have dramatic effect on profits Purchasing objectives: right product / variety, quality, quantity, price, time 1. Market research: to determine needs/wants of patients / prescribers, identify pharmacy image and business goals, space limitations, potential sales. Determining needs: usage reports, other pharmacies, pharmacy employees, questionnaires, sales reps, published top X drugs, formularies. Example: area with young families children items, older families elderly items. Consider special disease management areas, e.g. asthma, diabetes. 2. Effective purchasing policies: Use open-to-buy purchase budget. Control total $ investment in inventory. Use prior year data to forecast purchase budget for each month in the upcoming year, based on sales and COSG. Adjust ( / ) each month purchases based on previous month sales and purchases. Gross margin = sales COGS. 3. Selecting supply sources: must be dependable, prompt, frequent delivery, good return policy, frequency of out-of-stock, customer service, price, financing arrangement. Options: wholesales, manufactures, buying groups, rack jobbers, etc. Wholesales: advantages include storage of good until needed, rapid delivery, financing options, help with advertising promotions, store layout and design. Rack jobbers: stock and maintain a specified assortment of goods (e.g. eyeglasses) in a fixture in the pharmacy. Manufacturers: not common, large minimum purchases. Central purchasing groups: pool buying power of independent pharmacies for better terms. 4. Negotiating terms: price, discounts, dating, return policy. Pharmacy margin = suggested retail price pharmacy cost. Quantity discounts: cumulative (generic rebate) or non-cumulative ($ or % per quantity). Cash discount: for prompt payment (typical: 2% if paid in 10 days, net amount due in 30 days), or discount for Electronic Fund Transfer. Final price is calculated after subtracting trade, quantity and cash discounts. Dating: time for discount and payment (prepayment, collect-on-delivery (COD), delayed). Returned goods policy: full credit within x days, partial credit after y days, non-returnable after z days. Check shelves regularly for items not sold. Consider using a returned goods service company (charge a fee). 5. Transferring merchandise title (?) 6. Receiving, marking, stocking: count shipment, check for damage, check invoices, mark prices (merchandise, computer), stock. Stock depth considerations: average demand, review time, lead time, safety stock. Inventory control includes the following: 1. Visual: look at # of units in inventory and compare with how many should be carried, order more if needed. 2. Periodic: count stock on hand at certain intervals, compare to minimum target levels, order more if needed. 3. Perpetual: monitor inventory all the time (usually using a computer). Computer systems: sales, analysis, trends, perpetual, automatic ordering, interface inventory and dispensing systems at point of sale.
Profitability: the bottom line, important but not the only measure of success. Return on net worth = net profit / net worth. Target 25%. Net worth = total assets total liabilities. Net profit / net sales: target 5%. Net profit / total assets. Target 15%. Net profit / inventory. Target 20%. Expenses: salaries, wages, rent, utilities, accounting / legal fees, taxes, licenses, insurance, interest, equipment, depreciation. Balance sheet: includes assets and liabilities. Current assets: cash, account receivables, inventory. Current liabilities: account payable, accrued expenses.
Pricing
Components of price = ingredient cost + service cost (dispensing) + income. Actual Acquisition Cost (AAC): price the pharmacy pays for the product. Varies depending on source, volume, incentives and deals, type of pharmacy Average Wholesale Price (AWP): NOT (?) the average price the wholesalers sell the product at. Cost assigned to product by manufacturer, overstates AAC Estimated Acquisition Cost (EAC): established by third party payers to estimate AAC. Usually a percentage of AWP (e.g. 90%). Service cost: average or per unit cost of providing a service. Covers expenses such as salaries, rent, utilities, depreciation. Includes cost to dispense. Direct costs: results directly from providing the service. No direct cost if service is not provided. Dispensing direct costs: labels, containers, computer, delivery costs, patient education materials, pharmacy licenses. Indirect costs: costs shared by all services, e.g. rent, utilities, salaries, benefits, advertising, etc. Cost of providing a service = all direct costs + fair share of indirec t costs. Cost allocation: determining the fair share of indirect costs. Difficult. Estimate % of employees time and facility space devoted to dispensing. Cost to dispense (COD): total dispensing costs / expected Rx volume. It is an estimation of the average cost to dispense Rx. Sensitive to volume. Differential costs; differ among alternative courses of action, i.e. additional costs the pharmacy incurs for providing a new service. Non-cost factors: demand, competition, image, quality signaling, goals, non-monetary costs. Demand: quantity consumers will be at a certain price. Function of price. Elasticity of demand: measures sensitivity of demand to price . Elastic demand: small in price results in big in demand. Sellers make money by lowerin g the price. Inelastic demand is opposite ( price profit) Consumers are more sensitive to price when: cost of product is large part of total cost, differences among products, comparisons are easy, consumers can judge quality, switching costs are small, commodity. Image: consumers can select based on perceptions of pharmacy image. Image is affected by: prices, size, location, services offered, personnel, promotions, etc Price as a signal of quality: more likely when consumers cannot judge quality, more for services than products. Penetration pricing: price to sales volume. Loss leader pricing: Rx prices to OTC sales. Price skimming: price for superior service.
Basic Management
Management components: self, controllable surroundings, uncontrollable surroundings, external environment. Management activities: satisfy various entities, deal with emergencies, purchasing, recruiting, accounting, training, planning, negotiating, sales, dealing with regulatory officials. Management actions: identify tasks, organize resources, monitor performance / task completion, plan for future requirements, deal with problems. Functions of management actions: target setting, problem solving, leadership, team building, dealing with emergencies. Management functions: controlling, directing, organizing, planning, staffing
Controlling: establish standards based on objectives, measure / report performance, take corrective / preventative actions. Directing: motivation, communication, performance appraisal, discipline, conflict resolution. Organizing: division of labor, delegation of authority, departmentalization, span of control, coordination. Planning: vision, mission, objectives, coals Staffing: recruiting, selecting, hiring, training, retaining Know self, who we are, what we aspire to become, new info, what we need to know, who else need to work with us, etc. Managers skills: intellectual, technical, ethical, interactive, emotional. Intellectual skills: logical thinking, problem solving Ethical skills: define right from wrong Interactive skills: communicate intelligently and create an atmosphere that facilitates communication. Most problematic issues: poor communication, developing people, empowerment, lack of alignment, entitlement, balancing work / personal life, confronting poor performance, coaching senior management, cross-functional strife, fascination with programs. Decision making: identify objectives, analyze relevant factors, consider all alternatives, selection best option, implement the decision, evaluate the results Management style: depends on organization, situation, personal values, personality, chance. Self-development methods: observation, reflection, guided readings, attachments / visits, seeking feedback, seeking challenges. Strategic planning: must complement strategic thinking / acting. Includes where we are going (mission) and how we get there (strategy). SWOT analysis: strengths, weaknesses, opportunities, threats. Vision of success: mission, basic philosophy, core values, goals, strategies, performance criteria, decision rules, ethical standards. Environment: stability, complexity, market diversity, hostility, competition Cascade of information: should flow not only downward, but also upward Project management failures: lack of focus / attention, inability to cope with different project characteristics, feeling being used / exploited, lack of experience Project management process: develop ideas and proposals, approve the project, project kick-off / start, monitoring / reporting / managing, termination. Project management 10 commandments: concentrate on interfacing, organize project team, plan strategically / technically, remember Murphys law, identify stakeholders, manage conflict, expect the unexpected, listen to intuition, apply behavioral skills, take corrective actions. Project management functions: scope / quality / time / cost management PDCA Cycle: Plan, DO, Check, Act Problem solving: define the problem identify the criteria weight importance of criteria generate alternatives rate alternatives on each criterion compute the optimal decision Continuous Quality Improvement (CQI): philosophical / structural / healthcare-specific elements. Use PDCA cycle. Philosophical elements: strategic focus (mission, values, objectives), customer focus (patient, provider, payer), systems focus. Structural elements: process improvement teams, top management commitment, statistical analysis, customer satisfaction measures, benchmarking, seven tools (flow charts cause/effect diagrams, check sheets, histograms, etc). Healthcare specific elements: epidemiological studies, governance processes (QA, committees, peer review), risk-adjusted outcome measures, cost-effectiveness analysis. Barrier to quality transformation: lack of constancy of purpose, emphasis on short-term profits, personal view system, management mobility, using only visible figures, cost of employee healthcare, cost of warranty / insurance.
5. Extemporaneous Prescription Compounding 18. Nuclear Pharmacy 19. Pharmaceutical Care and Disease Management 21. Adverse Reactions and Post Market Surveillance 34. Clinical PK and Therapeutic Drug Monitroing 53. Renal Failure 57. Immunosuppressants in organ transplantation 58. Outcomes Research and Pharmacoeconomics Health care system
Preferred Provider Organization (PPO): Broad network of providers available, generally management is less strict. 52% Point of Service (POS): HMO plan with the option of going outside the narrow provider network if willing to pay higher cost-sharing. 18% HMO: Narrow choice of providers, tighter management. 26% Types of outcomes: Humanistic outcomes: Health Related Quality of Life, Patient Satisfaction, Caregiver Impact, Patient Preferences, Functional Status Economic: Cost Analysis, Cost-of-Illness, Cost-Minimization, Cost-Benefit, Cost-Effectiveness, CostUtility Clinical: Efficacy, Safety, Impact of therapy on natural history of the disease Methods for setting health insurance rates Experience rating: everyone in a specific area is charged the same premium based on the average cost of providing health services to all people in the area Community rating: premium adjusted individually according to a persons or groups average health history, risk, and past claim experience