01 - Current Draft WHO-CDC Biosafety Recommendations
01 - Current Draft WHO-CDC Biosafety Recommendations
01 - Current Draft WHO-CDC Biosafety Recommendations
Biosafety
The application of a combination of administrative controls, containment principles, laboratory practices and procedures, safety equipment, and laboratory facilities to enable laboratorians to work safely with potentially infectious microorganisms.
Administrative Controls
Supervision by an experienced scientist All personnel are well trained, proficient, aware of hazards, follow rules Routine medical surveillance Biosafety and operations manuals Emergency plans for spills, accidents, etc. Appropriate facilities and safety equipment
Containment
Primary Containment: protect worker and immediate laboratory environment good microbiologic techniques safety equipment Secondary Containment: protect the environment outside the laboratory facility design waste management
Risk Assessment
Pathogenicity of the infectious agent Route of transmission Agent stability and infectious dose Concentration of agent Type of laboratory procedures to be done Availability of effective prophylaxis or therapy Skill level and vulnerability of at-risk personnel
If a facility does not have all required BSL3 features (e.g. sealed penetrations, solid ceiling), an acceptable level of safety for conducting routine procedures, including culture, may be achieved in a BSL2 facility providing: Directional inward airflow is maintained and exhaust air is discharged to the outside Access to the laboratory is restricted when work is being performed The recommendations for BSL3 practices, procedures, and safety equipment are rigorously followed
Biosafety Guidance
Consensus recommendations for minimum biosafety requirements for Direct AFB-smear microscopy Processing specimens to concentrate bacilli for smear, culture, DST, molecular tests Manipulating cultures for smear, subculture, identification, DST, molecular tests Based on a risk assessment for each TB diagnostic procedure generation of infectious aerosols concentration of bacilli
References
CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 5th Edition. (2007) http://www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm Laboratory biosafety manual. 3rd edition, 2004. WHO. http://www.who.int/csr/resources/publications/biosafety/en/ Biosafety7.pdf Biosafety recommendations for the contained use of M. tuberculosis complex isolates in industrialised countries. P Herman et al, 2006. Scientific Institute of Public Health, Brussels, Belgium. http://www.biosecurite.be/CU/PDF/Mtub_Final_DL.pdf Interim Laboratory Biosafety Guidance for Extensively DrugResistant (XDR) M. tuberculosis strains. http://www.cdc.gov/tb/XDRTB/BiosafetyGuidance_xdrtb.htm
Acknowledgements
Biosafety Recommendations Vronique Vincent Peter van't Erve CN Paramasivan Philippe Dubois Chris Gilpin Sang Jae Kim Daniela Cirillo Shanna Nesby Jean Joly Thomas Shinnick Jenny Allen Andrew Ramsay John Ridderhof Karin Weyer Jon Crane May Chu Knut Feldmann Nicoletta Previsani Moses Joloba Sebastien Cognat Paul Jensen
Class II
Protects worker, product, environment used with aerosol-transmissible microorganisms, tissue culture
Class III
totally enclosed, ventilated, air-tight suitable for work with BSL3/4 agents