Monitor UV-900
Monitor UV-900
Monitor UV-900
GE Healthcare
Monitor UV-900
User Manual
ÄKTA
™
18112005ny.book Page 2 Wednesday, November 9, 2005 10:47 AM
18112005ny.book Page 3 Wednesday, November 9, 2005 10:47 AM
Contents
1 Introduction
1.1 General ................................................................................................7
1.2 Safety ...................................................................................................8
2 Installation
2.1 Unpacking ..........................................................................................9
2.2 General precautions .....................................................................9
2.3 Installing the flow cell ................................................................ 10
2.3.1 Fixing the flow cell .................................................................... 10
2.3.2 Connecting the optical fibers ............................................... 11
2.4 Connecting electrical signal cables .................................... 12
2.4.1 Connecting to recorder (if used).......................................... 12
2.5 Connecting to communication link ..................................... 12
2.6 Connecting to supply voltage ............................................... 13
2.7 Connecting the tubing .............................................................. 13
2.8 Mounting the cell holder cover ............................................. 14
3 Operation
3.1 On/off ................................................................................................ 15
3.2 Menu selection and settings .................................................. 15
3.2.1 Menu selection ........................................................................... 15
3.2.2 Return to main menu .............................................................. 16
3.2.3 Select value.................................................................................. 16
3.3 Main menu overview ................................................................. 17
3.4 Setting lamp on/off ..................................................................... 17
3.5 Reading absorbance values ................................................... 18
3.6 Setting wavelength ..................................................................... 18
3.7 Autozero .......................................................................................... 19
3.8 Storage and shut-down ........................................................... 19
3.9 Using an external chart recorder ........................................ 20
3.9.1 Setting range and zero ........................................................... 20
3.9.2 Event mark ................................................................................... 20
3.10 Filtering noise ................................................................................ 21
3.11 UV cell calibration ....................................................................... 22
3.12 Changing flow cell ....................................................................... 24
3.13 Restart after power failure ...................................................... 24
4 Maintenance
4.1 Periodic maintenance ............................................................... 25
Contents
5 Trouble-shooting
5.1 General .............................................................................................29
5.2 Faults and actions .......................................................................29
5.3 Error messages .............................................................................30
Reference information
A Description ........................................................................................................ 31
A.1 Instrument ............................................................................................................31
A.2 Flow cell .................................................................................................................32
A.3 Monitor principle ................................................................................................32
B Menus .................................................................................................................. 34
B.1 Check menu .........................................................................................................34
B.2 Setup menu ..........................................................................................................36
B.3 Setting and using the alarm timer .............................................................37
B.4 Service displays ..................................................................................................37
B.5 Menu overview ...................................................................................................38
C Technical specifications ............................................................................. 39
D Accessories and spare parts .................................................................... 42
Introduction 1
1 Introduction
1.1 General
Monitor UV-900 is a UV-absorption monitor for use in liquid chromatography.
Monitor UV-900 features:
• Variable wavelength for detection in the range 190–700 nm.
• Up to 3 wavelengths can be monitored simultaneously.
• Two alternative flow cells with path length 2 mm and 10 mm.
• Accurate and reliable monitoring through self-test, self-calibration and
fiber optics.
Monitor UV-900
1 Introduction
1.2 Safety
IMPORTANT! Monitor UV-900 is intended for laboratory use only, not for
clinical or in vitro use, or for diagnostic purposes.
WARNING! The instruments must not be opened by the user. They contain
high voltage circuits which can give a lethal electric shock.
CAUTION! The flow cell must not be used at pressures above 2 MPa (20 bar,
290 psi). At higher pressures the flow cell may break.
Installation 2
2 Installation
2.1 Unpacking
Unpack the instrument and check the items against the supplied packing list.
Inspect the items for obvious damage which may have occurred during
transportation.
• The system should be installed on a stable laboratory bench providing a
suitable working area.
• To maintain correct ventilation, the system requires an appropriate
amount of free space. Do not block the ventilation inlets or outlets on the
system!
It is recommended that all packing materials should be retained if onward
transport of the instrument is expected.
2 Installation
10 mm 2 mm
1 Unpack the flow cell. Do not remove the red protective caps from the inlet
or outlet, or the black protective caps on the optical fiber connectors.
2 Slide the rear white clip on the cell holder to its inner position for a 2 mm
cell and to its outer position for the 10 mm cell.
3 Place the flow cell in the opening between the white clips. The cell should
be positioned with the text and serial number facing upwards and to the
front. Press the cell into the clips to fasten it.
CAUTION! To avoid damaging the optical fibers, press only on the cell body,
never on the optical fibers.
Installation 2
To improve the access to the tubing connections, the flow cell can alternatively
be positioned with the tubing connections.
Normal position Alternative position
CAUTION! Do not touch the tips of the optical fibers with your fingers as
this will result in poor monitor performance. If you accidentally touch the
optical fiber tip, it can be wiped with 30% isopropanol using lens paper.
2 Remove the 2 rubber protective caps from the optical fiber receptacles on
the right side of the housing.
3 Connect the 2 optical fibers to the housing by carefully inserting them into
the sockets and tightening the nuts fingertight using the fiber detachment
Fiber
tool supplied.
detachment
tool
CAUTION! Do not twist the optical fibers during tightening.
Rubber sleeve
4 Slide the rubber sleeves on the 2 optical fibers onto the connectors. Make
sure that the sleeves are pushed tight to the housing to prevent dust or
fluid entering the connection.
2 Installation
WARNING! Do not block the rear panel of the system. The mains power
switch must always be easy to access.
Installation 2
Connect two UniNet cables to the UniNet-1 connectors. The instrument can be
connected in series anywhere in the chain between the PC and the termination
plug. The UniNet-1 link connects, in series, the PC with Pump P-905,
Monitor pH/C-900 and Monitor UV-900. The termination plug is connected to
the last instrument in the chain.
Inlet
Outlet
The inlet is on the top of the flow cell, the same side as the serial number.
Installation 2
Operation 3
3 Operation
3.1 On/off
Selftest 1 Switch on the instrument at the mains switch on the rear panel.
Please wait...
3 Operation
If a menu has sub levels, the sub menu is displayed by pressing the
OK button. Pressing the ESC button moves back one menu level.
Main menu 1
Main menu 2
OK OK
Main menu 3 Sub menu 3.1 Sub menu 3.1.1
ESC ESC
OK
Main menu 5 Sub menu 3.3 Sub menu 3.3.1
ESC
Main menu 1
ESC
Operation 3
3 Operation
or
215nm 254nm 280nm As an alternative all 3 wavelengths can be shown in a single display, but limited
1.123 0.023 0.123 to 3 decimals. This alternative is reached by turning the dial clockwise.
Set Wavelength λ2 4 The next wavelength λ2 is now shown. Set the value or turn the
(254nm) wavelength off, by turning the dial counter clockwise until the value passes
through 190 nm. If only one wavelength is to be used, λ2 and λ3 should be
set to off. Press OK.
The first wavelength λ1 can never be set to off.
5 Repeat step 4 in the menu Set Wavelength λ3.
6 Press ESC to return to the main operating menu.
In UNICORN, the wavelengths are set with the instruction Wavelength in
System Control:Manual:Alarm&Mon.
Operation 3
3.7 Autozero
The autozero function sets the detected absorbance to zero when the OK
button is pressed. All three wavelengths are autozeroed. Autozero is
recommended after wavelength changes in a method and before the sample
is injected.
Autozero 1 Select main menu Autozero, press OK. The normal absorbance value
display is then shown.
In UNICORN, Autozero is set with the instruction AutoZeroUV in System
Control:Manual:Alarm&Mon.
3 Operation
Set Range
2 Set the range value in the sub menu Set Range and press OK. The range is
(2.000AUFS) <0.1> the full scale absorbance range for the chart recorder (1 V). Only fixed
steps between 5.0 AU and 0.01 AU can be set and the value is the same for
all 3 wavelengths.
Set Zero Level 3 Select menu Set Zero Level, press OK.
(10% of FS) 20
4 Set the value and press OK. This value determines where the zero
absorbance level should be in relation to full scale on the recorder.
The instrument has an automatic overrange Measured absorbance
function. If during a peak the monitor signal level (A U)
reaches the full scale value, the signal will drop Range
AU
Overrange
Operation 3
3 Operation
Set UV Cell Length 1 To remove an old calibration value, press Esc while the display toggles
(--,--mm, SN------) between the two messages. The Set UV Cell Length menu appears.
Press OK.
Set Calibr. Length 2 At the Set Calibr. Length menu, set the cell length to 0. Press OK.
(--,--mm) --.--
Preparing the calibration
1 Remove any old calibration value by setting the cell length to 0 (see above).
2 Unpack the UV Test kit.
Union 3 Make sure that the Flow restrictor is connected in the flow path after the
UV flow cell.
4 Mount the Union Luer female/ 1/16" male, included in the test kit, in the
upper inlet of the UV flow cell.
Operation 3
6 The solution bottles are marked with the concentration value and the
reference absorbance value for each solution.
Enter the concentration of the solutions in increasing order in the column
UV Test kit Concentration (mg/l).
Enter the absorbance value in increasing order in the column
UV Test kit Absorbance (AU/cm).
Performing the absorbance measurements
For each of the four test solutions, the absorbance will be measured and
automatically compared to its reference value.
3 Operation
Set UV Cell Type 3 The Set UV Cell Type menu appears. Check the cell type value.
(2 mm)
If the value is correct, turn the selection dial clockwise to go to next menu.
If the value has to be changed, press OK to change it.
4 At the Set Cell Serial No. menu press OK.
Set Cell Serial No. 5 Enter the serial number of the cell (see the label on the flow cell). Press OK .
(SN ------) ------
Maintenance 4
4 Maintenance
WARNING! Remove liquid or dirt from the system surface using a cloth and,
if necessary, a mild cleaning agent.
WARNING! When using hazardous chemicals, make sure that the entire
system has been flushed thoroughly with bacteriostatic solution, e.g. NaOH,
and distilled water before service and maintenance.
4.2 Cleaning-in-place
Pump a cleaning or sanitizing agent through the flow cell. The standard
recommendation is to pump 1 M NaOH at 1 ml/min for 30 minutes and then
wash out with buffer.
Maintenance 4
Maintenance 4
WARNING! When using hazardous chemicals, make sure that the entire
system has been flushed thoroughly with bacteriostatic solution, e.g. NaOH,
and distilled water before service and maintenance.
4 Maintenance
4.6 Recycling
This symbol indicates that the waste of electrical and electronic equipment
must not be disposed as unsorted municipal waste and must be collected
separately. Please contact an authorized representative of the manufacturer
for information concerning the decommissioning of equipment
Trouble-shooting 5
5 Trouble-shooting
5.1 General
Monitor UV-900 When contacting GE Healthcare for support, state the program version of the
V1.06 instrument, which is shown for 2 seconds after the self-test, during switch-on,
or in the menu Check Service Mode, see page 35.
WARNING! The instruments must not be opened by the user. They contain
high voltage circuits which can give a lethal electric shock.
5 Trouble-shooting
Message Action
Sample fiber failure There may be too high absorption in the UV cell.
1. Check the liquid.
2. Ensure that there are no air bubbles in the system.
3. Check the connections of the UV cell optical fibers.
4. Clean the UV cell, see section Cleaning the flow cell and
optical fiber connectors.
Not calibrated Recalibrate by switching the instrument off and then back to
Calibration failed on, or choose Recalibrate in the Check menu
Reference information A
Reference information
A Description
A.1 Instrument
The Monitor UV-900 is a UV-absorption monitor with variable wavelength. The
instrument contains no internal user replaceable items.
Monitor UV-900
Connector/switch Function
Analogue out 0–1 V Recorder output, 3 channels 0–1 V
Remote Digital signal inputs, lamp on/off, autozero, event mark
UniNet 1 Computer network
Mains Supply voltage, grounded
On/off Instrument on/off switch
Connect any auxiliary equipment to the 9-pole D–Sub female REMOTE
connector (5 V TTL signals only).
Pin Signal Function
Active status = low or closed terminal to pin 5 (0V)
1 Remote on/off Active = lamp on, inactive = lamp off
5 4 3 2 1 2 Autozero From inactive to active > 100 ms = Autozero
9 8 7 6 3 Event mark From inactive to active > 100 ms = Event mark
4 –
5 0V
6–9 –
A Reference information
The unique design of the flow cell prevents the formation of distinct interfaces
between eluent components with different refractive indices and eliminates
the negative influence these would cause. The precision of monitoring is
enhanced by the construction of the flow cell, which ensures total reflectance
of light. This maintains a high intensity of light to the detector. The long path
length combined with a small cell volume increases sensitivity.
A.3 Monitor principle
A Xenon flash lamp gives a high intensity, continuous spectrum throughout the
range 160–2000 nm. The light enters a monochromator which includes a
condensing system, blocking filter, entrance slit and a concave aberration-
corrected holographic grating. Monochromatic light from the grating is
directed to an optical fibre. The grating is turned by a stepping motor for
wavelength selection between 190–700 nm, in steps of 1 nm. Up to 3
wavelengths can be monitored simultaneously.
For wavelengths between 360–700 nm, a blocking filter is moved into the light
path to filter out unwanted light, of a half wavelength from the second order
spectrum, before entering the monochromator.
The light from the monochromator to the flow cell and from the cell to the
detector electronics is guided by optical fibres which focus its full intensity on
the liquid flow path, maximising the sensitivity of the monitoring. Before
entering the flow cell, the monochromatic light is split in a beam splitter, with
50% of the light passing through the sample fibre (S) and the flow cell, and 50%
being directed through the reference fibre (R). Two photodiodes with identical
characteristics monitor the intensities of the measuring and reference beams.
Reference information A
Monochromator Detectors
and electronics S
Flow cell
Block Beam
filter splitter
Con-
densor
The long path length and small volume of the flow cell ensure very high
sensitivities and high signal-to-noise ratios. The detection system is also very
stable and because the optical unit is located away from the lamp and
electronics, noise and drift caused by temperature variations is avoided.
At calibration the instrument automatically finds 2 persistent lines in the
spectrum of Xenon. The wavelengths of these known lines are used to calibrate
the stepper motor that turns the grating.
B Reference information
B Menus
Wavelengths not OK
230-4 459+3 542+4 OK
Wavelengths not OK
Recalibrate? OK
Recalibrate
If the instrument is left switched on for a long period (> 10 days), it may be
necessary to recalibrate it. This calibration is identical to that done when the
instrument is switched on.
1 Select main menu Check, press OK.
Recalibrating 2 Select sub menu Recalibrate, press OK. The recalibration starts and after
Please wait...
approx. 60 seconds the message Recalibration finished is shown.
Recalibration
finished OK
Reference information B
Check Service Mode 2 Select sub menu Check Service Mode by pressing OK.
Telephone Service 3 The service telephone number is displayed. Turn the selection dial
012345678901 clockwise to view the next display.
Contract Number 4 The service contract number is displayed. Turn the selection dial clockwise
012345678901 to view the next display.
Serial Number 5 The instrument serial number is displayed. Turn the selection dial
01234567 YM 012345 clockwise to view the next display.
Monitor UV-900 6 The instrument name and software version are displayed. Turn the
V1.06 selection dial clockwise to view the next display.
Date of Maintenance 7 The date of the last service is displayed. Turn the selection dial clockwise
? to view the next display.
B Reference information
Reference information B
Alarm/ Timer Action 2 Set the action to take place. Press OK to select action. Buzzer will generate
(Bzz) Buzzer Run End an audible alarm for 15 s and a message. Run will start the pump at the
set flow rate, End will stop the pump, each generating one beep and a
message.
Set Alarm 12:32:22 3 Use the sub menu Set Alarm if you want to set an alarm at a fixed time.
(0) 00.00.00 Press OK to enter the time value in the form HH.MM.SS, pressing the OK
button after entering each time unit.
Set Timer 4 If you want to set a count-down timer, turn the dial to select sub menu Set
(0) 00.00.00 Timer. Press OK to enter the countdown value in the form HH.MM.SS,
pressing the OK button after entering each time unit.
Alarm/Timer 12:30:52 5 Press ESC button to return to the Alarm/Timer menu which now shows the
(Bzz 12:33:00) set alarm time or count-down time as BzzHH:MM:SS.
Bzz12:41:29 12:41:49 6 When the alarm time is due or the count-down timer reaches 00:00:00, an
! ! Alarm time ! ! alert display is shown and the instrument beeps, until the OK button is
pressed.
Set Clock The alarm timer is based on the internal instrument clock which can be set in
00:32:22 (00:36:53) the Set Clock menu placed after the Alarm/Timer menu. The clock will be reset
when power is turned OFF.
Alarm/Timer off? An already set alarm/timer function can be reset by pressing OK in the menu
(Bzz 05:33:00) Alarm/Timer off?
B.4 Service displays
The instrument has service displays for use by authorised service personnel. If
the service display Enter Access Code: is accidentally selected, press the ESC
Enter Access Code!
B Reference information
Set Wavelength
(215, 254, 280 nm) Set Calibr. Length Set Calibr. Length
(--.--mm) (--.--mm) --.--
Set UV Cell Type Set UV Cell Type
(2 mm) (2 mm) 2
Set Analogue Out
Set Cell Serial No. Set Cell Serial No.
(2.000AUFS, 10%)
(SN------) (SN -------) ------
Reference information C
C Technical specifications
Operating data
Wavelength range 190–700 nm in steps of 1 nm,
3 wavelengths simultaneously
Bandwidth 4 nm
Wavelength accuracy ±2 nm
Wavelength reproducibility ±0.01 nm
Wavelength switch time < 500 ms (one cycle from 214 nm to
254 nm and back to 214 nm)
Linearity < 2% deviation up to 2 AU at 260 nm with
Uracil at pH 2
Noise (specified for 10 mm
flow cell) 1, 2
Single wavelength
short term (0.5–1 min) < 6x10 AU at 230 nm
-5
Dual wavelengths
short term (0.5–1 min) < 2x10 AU at 230 and 254 nm
-4
Drift 2)
< 2x10 AU/h at 254 nm
-4
Environment +4 to +40 °C
20–95% relative humidity
84–106 kPa (840-1060 mbar)
atmospheric pressure
1)
Measured with water at 1 ml/min, time constant 1 s.
2)
Typical values at room temperature after varm-up.
Flow cell
Max. flow rate 100 ml/min
Max. pressure 2 MPa (20 bar, 290 psi)
Backpressure Max. 0.5 bar at 2 ml/min with water at
25 °C
Liquid temperature range +4 to +40 °C
Optical path length
2 mm cell 2 mm
10 mm cell 10 mm
Cell volume
2 mm cell 2 µl
10 mm cell 8 µl
Degree of protection IP 21
Wetted materials PTFE (polytetrafluoroethylene)
PEEK (polyetheretherketone)
Titanium (palladium alloy)
Quartz (synthetic fused silica)
pH stability range 1–13, 1–14 (<1 days exposure)
C Reference information
Reference information C
D Reference information
Short instructions
The following short instructions are intended as a
guide for users who are fully familiar with the safety Monitor UV-900
Select OK ESC
Set Wavelength λ1
(215nm)
Set Wavelength λ2
(254nm)
Set Wavelength λ3
(280nm)
λ3(280) 1.123 AU
Monitor UV-900
User Manual 18-1120-05 Edition AF 43
18112005ny.book Page 44 Wednesday, November 9, 2005 10:47 AM
18112005ny.book Page 45 Wednesday, November 9, 2005 10:47 AM
18112005ny.book Page 46 Wednesday, November 9, 2005 10:47 AM
Asia Paci.c Tel: +852 2811 8693 Fax: +852 2811 5251 • Australasia Tel: + 61 2 9899 0999 Fax: +61 2 9899 7511 • Austria Tel: 01/57606-1619 Fax: 01/57606-1627 • Belgium Tel: 0800 73 888
Fax: 03 272 1637 • Canada Tel: 800 463 5800 Fax: 800 567 1008 • Central, East, & South East Europe Tel: +43 1 982 3826 Fax: +43 1 985 8327 • Denmark Tel: 45 16 2400 Fax: 45 16 2424 •
Finland & Baltics Tel: +358-(0)9-512 39 40 Fax: +358 (0)9 512 39 439 • France Tel: 01 69 35 67 00 Fax: 01 69 41 96 77 • Germany Tel: 0761/4903-490 Fax: 0761/4903-405 • Italy Tel: 02 27322 1
Fax: 02 27302 212 • Japan Tel: +81 3 5331 9336 Fax: +81 3 5331 9370 • Latin America Tel: +55 11 3933 7300 Fax: +55 11 3933 7304 • Middle East & Africa Tel: +30 210 9600 687
Fax: +30 210 9600 693 • Netherlands Tel: 0165 580 410 Fax: 0165 580 401 • Norway Tel: 815 65 555 Fax: 815 65 666 • Portugal Tel: 21 417 7035 Fax: 21 417 3184 • Russia & other C.I.S. & N.I.S
Tel: +7 (095) 232 0250, 956 1137 Fax: +7 (095)230 6377 • South East Asia Tel: 60 3 8024 2080 Fax: 60 3 8024 2090 • Spain Tel: 93 594 49 50 Fax: 93 594 49 55 • Sweden Tel: 018 612 1900
Fax: 018 612 1910 • Switzerland Tel: 0848 8028 12 Fax: 0848 8028 13 • UK Tel: 0800 616928 Fax: 0800 616927 • USA Tel: 800 526 3593 Fax: 877 295 8102
5
0
0
imagination at work
2
s
r
e
t
f
o
T
s
r
e