BMLT Syllabus JISU

JIS UNIVERSITY
[Syllabus for Bachelor of Medical Laboratory Technology (BMLT) Course]
SYLLABUS FOR THE

BACHELOR OF MEDICAL LABORATORY TECHNOLOGY
(BMLT) COURSE
81, Nilgunj Road, Jagarata Pally, Deshpriya Nagar, Agarpara, Kolkata-

700109
West Bengal
JIS UNIVERSITY
B.M.L.T.
Bachelor of Medical Laboratory Technology
Duration: 3 Years (6 semesters)

Level: Graduation
Type: Degree
Eligibility:10+2 (Science)
B.M.L.T. or Bachelor in Medical Laboratory Technology is an undergraduate Medical

Lab Technologist Programme. Bachelor in Medical Laboratory Technology program aims to
provide the aspirant withsufficient knowledge and skills to carry out routine laboratory
diagnostic procedures and somesophisticated laboratory diagnostic procedures. The course
deals with new advances in Management ofLaboratories, instrumentation etc. The duration of
the course is three years and it is career orienting innature and its syllabus is divided into six
semesters.
Bachelor in Medical Laboratory Technology Eligibility

Students should have passed in class 12th in Science with minimum 50% (45% for SC/ST) or
equivalent CGPA from a recognized board.
Bachelor in Medical Laboratory Technology Course Suitability

Candidates should have possessed a good analytical judgment and the ability to work under
pressure.They also have a close attention to detail which is essential for Medical Laboratory
Techniciansbecause small differences or changes in test substances or numerical readouts can
be crucial to adiagnosis.Students should have normal color vision are highly desirable, and
able to handle automatedlaboratory equipment, computer skills are important.
How is Bachelor in Medical Laboratory Technology Course Beneficial?

Bachelor in Medical Laboratory Technology course offers skill and knowledge progress in
applyingmedical standards and technical standards for assessing a lab test.The program will
help the aspirants to work in the research area as assistants.After passing their graduation
they can go for further master’s degree than for research work; theycan also become teachers
in schools.
JIS UNIVERSITY
Bachelor in Medical Laboratory Technology Employment Areas
Clinics
Commercial Laboratories
Hospitals
Nursing Homes
Public Health Facilities
Bachelor in Medical Laboratory Technology Job Types
Junior Technical Executive

Lab Medicine Technician
Laboratory Technician
R&D Contractual Lab Assistant
System Analyst
X-Ray Technician
Advance Courses in Bachelor in Medical Laboratory Technology
Post Graduate Diploma in Medical Laboratory Technology
M.Sc. (Medical Lab Technology)

JIS UNIVERSITY
Syllabus of Medical Lab Technology as proposed by JIS University will be as follows. The
courses will be distributed in six (6) semesters.
Year I
Sr. No. Subjects of Study
1 Human Anatomy and Physiology
2 General Microbiology
3 Pathology
4 General Biochemistry
5 Fundamentals of Computer Science
6 Communication for Professionals
7 Human Anatomy and Physiology (P)
8 General Microbiology (P)
9 Pathology (P)
10 General Biochemistry (P)
Year II
1 Biomedical Waste Management
2 Bacteriology, Immunology and Parasitology
3 Clinical Biochemistry
4 Hematology and Blood Banking
5 Basic and Clinical Pharmacology
6 Health education and health communication
7 Community Medicine
8 Bacteriology, Immunology and Parasitology (P)
9 Clinical Biochemistry (P)
10 Hematology and Blood Banking (P)
Year III
1 Histotechnology
2 Virology, Mycology and Applied Microbiology
3 Essentials of Medical Pharmacology
4 Histopathology and Cytopathology Techniques
5 Biomedical Techniques, Lab Management and Ethics
6 Diagnostic Molecular Biology
7 Virology, Mycology and Applied Microbiology (P)
8 Histopathology and Cytopathology Techniques (P)
9 Diagnostic Molecular Biology(P)
10 Hospital Training
JIS UNIVERSITY
The syllabus shall come into effect from the academic year 2019-20 under JIS
University. The course of study for BMLT shall include semester wise theory and
practical as given in table I to VII. The no. of hours to be devoted to each theory and
practical course in any semester shall not be less than shown in table I to VII.
COURSE STRUCTURE
1STYEAR
SEMESTER-I
Period Credit
Sl. Course Code
Subject
L T P Total
1 BML-101 Human Anatomy and Physiology-I 3 - - 3
(Theory)
2 BML-102 3 - - 3
Pathology-I (Theory)
3 BML-103 3 - - 3
General Microbiology-1 (Theory)
4 BML-104 3 - - 3
Biochemistry-1 (Theory)
5 BML-105 Communication for Professionals 3 - - 3
(Theory)
6 BML-191 Human Anatomy and Physiology-I - - 3 2
(Practical)
7 BML-192 Pathology-I (Practical) - - 3 2
8 BML-193 General Microbiology-I (Practical) - - 3 2
9 BML-194 Biochemistry-I(Practical) - - 3 2
10 BML-181 Skill-X - - - 1
11 BML-182 Seminar and GD - - - 1
Total 23 + 2 = 25
JIS UNIVERSITY
SEMESTER-II
Course Period Credit

Sl. Subject
Code
L T P Total
1 BML-201 Human Anatomy and Physiology- II 3 - - 3

(Theory)
2 BML-202 Pathology-II (Theory) 3 - - 3
3 BML-203 General Microbiology-II (Theory) 3 - - 3
4 BML-204 Biochemistry-II (Theory) 3 - - 3

5 BML-205 Fundamentals of Computer Science 3 - - 3
(Theory)
6 BML-291 Human Anatomy and Physiology- - - 3 2
II (Practical)
7 BML-292 Pathology-II (Practical) - - 3 2
8 BML-293 General Microbiology-II (Practical) - - 3 2
9 BML-294 Biochemistry-II (Practical) - - 3 2
10 BML-281 Skill-X - - - 1
Total 23+2 = 25
JIS UNIVERSITY
2NDYEAR
SEMESTER III
Period Credit
Course
Sl. Subject L T P Total
Code
1 BML-301 3 - - 3
Hematology and Blood Banking-I(Theory)
2 BML-302 3 - - 3
Clinical Biochemistry-I (Theory)
3 BML-303 3 - - 3
Basic and Clinical Pharmacology (Theory)
4 BML-304 Health education and health communication 3 - - 3
(Theory)
5 BML-305 3 - - 3
Biomedical Waste Management (Theory)
7 BML-391 - - 3 2
Hematology and Blood Banking-I(Practical)
8 BML-392 Clinical Biochemistry-I (Practical) - - 3 2
9 BML-381 Skill-X - - - 1
Total 19+2 = 21
JIS UNIVERSITY
SEMESTER IV
Period Credit
Sl. Course Code Subject L T P Total
1 BML-401 3 - - 3
Hematology and Blood Banking-II(Theory)
2 BML-402 Clinical Biochemistry-II (Theory) 3 - - 3
3 BML-403 Bacteriology, Immunology and Parasitology 3 - - 3
(Theory)
4 BML-404 3 - - 3
Community Medicine (Theory)
5 BML-491 - - 3 2
Hematology and Blood Banking-II (Practical)
6 BML-492 - - 3 2
Clinical Biochemistry-II (Practical)
7 BML-493 Bacteriology, Immunology and Parasitology - - 3 2
(Practical)
8 BML-481 Skill-X - - - 1
Total 18+2 =20

JIS UNIVERSITY
3rd YEAR
SEMESTER V
Course Period Credit

Sl. Subject L T P Total
Code
Virology, Mycology and Applied Microbiology
1 BML-501 3 - - 3
(Theory)
Histopathology and Cytopathology Techniques
2 BML-502 3 - - 3
(Theory)
3 BML-503 Histotechnology (Theory) 3 - - 3
4 BML-504 Clinical enzymology and automation (Theory) 3 - - 3
BML-591 Virology, Mycology and Applied Microbiology

5 - - 3 2
(Practical)
BML-592 Histopathology and Cytopathology Techniques
6 - - 3 2
(Practical)
9 BML-581 Skill-X - - - 1
`10 BML-582 Seminar and GD - - - 1
Total 16 + 2 = 18
JIS UNIVERSITY
SEMESTER VI
Period
Course Credit
Sl. Subject
Code L T P Total
1 Diagonostic Molecular Biology (Theory) 3 - - 3

BML-601
2 BML-602 Essentials of Medical Pharmacology (Theory) 3 - - 3
3 BML-603 Biomedical Techniques, Lab Management and Ethics 3 - - 3

(Theory)
4 BML-691 Diagonostic Molecular Biology (Practical) - - 3 2
5 BML-692 Hospital Training - - - 6
6
BML-681 Skill-X - - - 1
Total 17+2 = 19
JIS UNIVERSITY
SYLLABUS FOR BACHELOR OF MEDICAL LABORATORY

TECHNOLOGY (BMLT)
BMLT
ST
1 YEAR
JIS UNIVERSITY
SEMESTER I
BML 101: HUMAN ANATOMY AND PHYSIOLOGY-I (THEORY) 45 Hours
Course Content
Unit -1
Terminology and General Plan of the Body, Body Parts and
Areas,TermsofLocationandPosition,BodyCavitiesandTheirMembranes,Dorsalcavity,Ventral
cavity, Planes and Sections
Unit –II
Cells: Structure, function and location, Prokaryotic and eukaryotic cells, Cell organelles, Cell
division. Tissue, Types, Structure, Location and Function of Epithelial Tissue, Connective
Tissue, Muscle Tissue, Nerve Tissue, Membranes, Glandular tissue.The Integumentary
System: structure and function of The Skin, Subcutaneous Tissue
Unit-III
Cell physiology: Structure, membrane, transport across cell membrane, Active, Passive,
Organization of the Body, Body Composition, Body Fluid Volumes and itsmeasurement,
Diffusion, Osmosis, Tonicity, Homeostasis
Unit-IV
Blood-composition, function, cellular component & their function, haemoglobin&anaemia, blood
groups and coagulation. Lymphatic system-Composition & function of lymph, lymphatic tissue,
Immunity with the role of thymus
Unit-V
Musculoskeletal System: Basic anatomy of important muscles and bones,Structure of
skeletal muscle. Muscle contraction andrelaxation.
Unit-VI
Cardiovascular system-general arrange, heart, arteries, veins and capillaries, heart structure and
function, cardiac cycle, heart sounds, heart rate, blood pressure,mechanism of circulation,
definition of hypertension & shock
Unit-VII
Respiratory system: parts of respiratory system, mechanism of respiration, pulmonary
function, pulmonary circulation, lungs volume, Gas transport between lungs and
tissues,Definition of hypoxia, dyspnoea, cyanosis, asphyxia and obstructive airways diseases
BML 191: HUMAN ANATOMY AND PHYSIOLOGY-I (Practical) 3 Hours/week

JIS UNIVERSITY
1. Demonstration of Major organs through models and permanentslides.

2. Demonstrationofpartsofcirculatorysystemfrommodels.
3. Demonstrationofpartsofrespiratorysystemfrommodels.
4. Demonstrationofstructuraldifferencesbetweenskeletal,smoothandcardiacmuscles.
5. Demonstrationofvariousbones
6. Demonstrationofvariousjoints
7. To measure pulserate
8. To measure bloodpressure
9. Demonstration ofECG
10. To perform Hemoglobin by Sahli’sMethod
11. To perform Hemoglobin by CMGmethod.
Recommended Books (Latest Editions)

JIS UNIVERSITY
BML 102: PATHOLOGY I (Theory) 45 Hours
Course content
Unit I
Introduction & History of pathology, Basic definitions and familiarization with the common
terms used in pathology, Causes and mechanisms of cell injury, reversible and irreversible
injury, Introduction of hyperplasia, hypoplasia, hypertrophy, atrophy, metaplasia, necrosis
andapoptosis
Unit II
General features of acute and chronic inflammation: Vascular changes, cellular events, Cells
and mediators of inflammation, Phagocytosisand its mechanism
Unit III
Tissue Renewal and Repair, healing and fibrosis, cirrhosis, introduction of oedema,
hyperaemia, congestion, haemorrhage, haemostasis, thrombosis, embolism, infarction,
shock and hypertension.
Unit IV
Protein energy malnutrition, deficiency diseases of vitamins and minerals, nutritional excess
and imbalances. Role and effect of metals (Zinc, Iron and Calcium) and their deficiency
diseases, Aetiology and pathophysiology of diabetes, arteriosclerosis, myocardial infarction,
respiratory diseases (COPD), Parkinson disease, Infectious Diseases: pathogenesis &
overview of modes of infections, prevention and control with suitable examples like
Typhoid, Dengue
Unit V
Cancer: Definitions, nomenclature, characteristics of benign and malignant neoplasm,
metastasis, Carcinogens and cancer, concept of oncogenes, tumour suppressor genes, DNA
repair genes and cancers stem cells.
BML 102: PATHOLOGY-I (Practical) 3 Hours/week
1. Haemoglobin by CMGmethod.
2. To perform Total RBCcount.
3. To perform total leucocytecount.
4. To perform differential leucocytecount.
5. To performPCV
6. To calculate Red cellindices.
7. To perform total plateletcount.
8. To perform bleedingtime.
9. To perform clottingtime.
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10. To study about CSFexamination.
11. Microscopic examination ofurine
12. Examination ofurine
13. Examination ofsputum

JIS UNIVERSITY
BML 103: GENERAL MICROBIOLOGY-I (Theory) 45 Hours
Course content
Unit-I
Development of microbiology as a discipline, Contributions of Anton von Leeuwenhoek,
Louis Pasteur, Robert Koch, Joseph Lister, Alexander Fleming, Edward Jenner, Introduction
to bacterial taxonomy, Classification of Bacteria, Morphology based onsize, shape,
arrangement, motility, flagella, spores, capsules, cell wall, plasma membrane, pili, and
ribosomes.
Unit-II
Microscopy: Study of compound microscope – magnification, numerical aperture, resolution
and components of microscope. Dark ground illumination, care of microscope and common
difficulties micrometry. Bright Field Microscope, Dark Field Microscope, Phase Contrast
Microscope, Fluorescence Microscope,Transmission Electron Microscope, Scanning
Electron Microscope
Unit-III
Cell size, shape and arrangement, cell-wall, composition and detailed structure of Gram-
positive and Gram-negative cell walls, Cell Membrane: Structure, function and chemical
composition of bacterial cell membranes. Cytoplasm: Ribosome,mesosomes, inclusion
bodies, nucleoid, chromosome and plasmids, Endospore: Structure, formation
Unit-IV
General safety measures used in Microbiology laboratory, Sterilization and disinfection:
Various physical methods of sterilization – heat, UV radiation, ionizing radiation, filtration,
characters affecting sterilization, auto clave control and sterilization indicators.Biomedical
waste management in a Medical Microbiology laboratory: Types of the waste generated,
Segregation, Treatment, Disposal
Unit-V
Antiseptics & Disinfectants: Definition, types and properties, mode of action, use, qualities
of good disinfectants. Chemical disinfectants – phenol and its compounds, alcohol, halogen,
heavy metals and quaternary ammonium compounds, aldehyde, gaseous compound. use and
abuse of disinfectants. Precautions while using the disinfectants.
BML 193: GENERAL MICROBIOLOGY-I (Practical) 3 Hours/week
1. Demonstration of Autoclave and sterilization ofmedia

2. Demonstration of Laminar air flow and mediapreparation
3. Preparation of cultureplates
4. Demonstration ofCentrifuge.
5. Demonstration of hot air Oven and sterilization ofglassware’s
6. Demonstration of Incubator and preservation ofcultures
7. Preparation ofmedia
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8. Antibiotic sensitivitytest.

JIS UNIVERSITY
BML 104: BIOCHEMISTRY-1 (Theory) 45 Hours
Course content
Unit-I
Carbohydrate – Definition, Source, Classification, Functions and Importance, Physiological
importance of major type of carbohydrates. Carbohydrate metabolism – Glycolysis, HMP
shunt, TCA cycle, Glycogenesis, Glycogenolysis, Neoglucogenesis, Blood sugar level
Unit-II
Protein – Definition, Source, Classification, Function and Importance of major type of
proteins. Protein metabolism – Transamination, Transmethylation, Deamylation, Urea
synthesis, Inborn error of metabolism.
Unit-III
Lipids - Definition, Source, Classification, Function of major type of lipids. Saturated and
Unsaturated type of fatty acids, Essential fatty acids and their importance.Phospholipids and
their importance.Lipid metabolism – Fatty acid oxidation, Ketone bodies, Metabolism of
cholesterol, Arteriosclerosis and Obesity.
Unit-IV
Enzymes : Definition, Classification of enzyme, Cofactor & Coenzymes, Concept of active
sites and general mode of action of enzymes, units for measuring enzyme activity, factor
affecting enzyme activity, factor responsible for abnormal enzyme secretion
Unit-V
Nucleic acids: Structure, Function and types of DNA and RNA, Nucleotides, Nucleosides,
Nitrogen bases, purines and pyrimidines and role of Nucleic acid.
Unit-VI
Vitamins: classification, function and disease associated with vitamins.Minerals and ions:
Requirement, function and biological importance of Calcium, Iron, Iodine, Zinc, Phosphorus,
Copper, Sodium and Potassium
BML 194: BIOCHEMISTRY-I (Practical) 3 Hours/week
1. To determine protein by Biuretmethod.

2. To perform protein test by variousmethods.
3. Physical examination ofurine
4. Urine sugar determination by Benedict’smethod.
5. Protein by heat and aceticmethod
6. Bile salt, Bile pigments and Urobilinogendetermination
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7. Determination of Ketonebodies
8. Determinationofvariousparametersofurine byuristikmethod.
9. Preparation ofhemolysate
10. To identifycarbohydrates in given solution by various methods.

JIS UNIVERSITY
BML 195: COMMUNICATION FOR PROFESSIONALS (Theory) 45 Hours
Course content
1. Introduction: Meaning of Communication; Role of Communication in Business; Basic

elements of the Communication process, level of Communication, forms, models and
media of Communications, Verbal and non-verbal Communication-functions and types.
Barriers to effective Communication.
2. Grammar: Subject verb agreement, tense, voice, improvement of sentences,
rearrangement of sentences. Vocabulary: usage, synonyms,antonyms.
3. Comprehension
4. Forms of Writing: The Essay, The Précis, The Report, The Proposal, The C.V. andJob
5. Application letter. ThePresentation.
6. RolePlaying
7. GroupDiscussion

JIS UNIVERSITY
SEMESTER II
BML 201: HUMAN ANATOMY AND PHYSIOLOGY-II (THEORY) 45 Hours
Course content
Unit-I
Digestive system: basic anatomy of esophagus, stomach, small intestine, large intestine,
liver, gall bladder, pancreas
Unit-II
Gastrointestinal physiology: Organs of GIT and their structure & function, secretion,
digestion, absorption and assimilation, gastrointestinal hormones, physiology of digestion
of carbohydrates, proteins & lipids, Structure & function of liver, spleen,
gall bladder & pancreas, Jaundice, Cirrhosis & Pancreatitis
Unit-III
Endocrine system: Different hormones in endocrine system. Action of pituitary, thyroid,

parathyroid, adrenal and gonadal hormones. Body temperature regulatory process in human -
role of endocrine and nervous system.
Unit-IV
Neurophysiology: Reflex system, automatic nervous system, parts of brain and function of each
part. Nerve tract and their role.
Unit-V
Special senses: Structure of retina, rhodopsin and iodopsin cycle, visual tract, accommodation.
Auditory tract, mechanism of audition. Structure of taste bud, taste pathway, Olfaction and its
physiology.
Unit-VI
Renal physiology: Structure and function of renal system. Urine formation, micturition, renal
clearance test, renal buffer system.
Unit-VII
Reproductive system: Male and female reproductive organs, Gametogenesis, Ovulation,

Menstrual Cycle.
JIS UNIVERSITY
BML 291 HUMAN ANATOMY & PHYSIOLOGY-II (Practical)

3 Hours/week
1. Demonstration of digestivesystem from models.

2. Demonstration of excretory system from models.
3. Demonstration of nervous system from models.
4. Structure of eye and ear
5. Demonstration of various parts of male & female reproductive system from
models
JIS UNIVERSITY
BML 202: PATHOLOGY-II (THEORY) 45 Hours
Course content
Unit- I
Hemoglobin, structure ,function and types , Hemoglobinometry , Haemoglobin estimation by
various methods, advantages and disadvantages, physiological and pathological variations on
blood parameters, Hemocytometry, visual and electronic method, neubauer counting
chamber, RBC count, WBC count, Platelets count,
absoluteeosinophilcount,principle,procedure,calculation,significance,precautions involved
during counting, absolute count of various WBCs. Physiological and pathologicalchanges
invalues, Erythrocyte sedimentation rate, manual and automated method, factor affecting
ESR, packed cell volume, red cell indices (MCV, MCH, MCHC), Physiological and
pathological variations invalue
Unit-II
Complete blood count, determination by automated method and significance of each
parameter, Reticulocyte count, routine examination of CSF, semen, sputum and stool.
Unit –III
Mechanism of coagulation, coagulation factors, Bleeding time, clotting time, platelet count,
protamine sulphate test, clot retraction test
Unit-IV
Introduction to immuno hematology and blood banking technology, antigen, antibody,
complements,ABO & Rh blood group system, method of determination, other blood group
system, Donor selection, blood collection, anticoagulants, additive systems, blood bags, its
labelling , storage and transportation
Unit- V
Uses,care & maintenance and calibration of Coulter counter, coagulometer,automatic ESR
analyzer, urine analyzer, point of caretesting.Pre and Post analytical variables, automation in
hematology
BML- 292 PATHOLOGY-II (LAB) 3Hours/Week
1. To perform ELISAtest.To perform TB IgG&IgMtest

2. To perform Dengue IgG&IgMtest
3. To demonstrate agglutinationreaction.
4. To perform RAtest
5. To perform WIDALtest
6. To study about intrauterine contraceptivedevices.
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7. To demonstrate microscopic structure of bones withpermanentslides.
8. To demonstrate microscopic structure of muscles with permanentslides.
JIS UNIVERSITY
BML 203: GENERAL MICROBIOLOGY-II (THEORY) 45Hours
Course content
Unit-I
Lab organization, management, recording of results and quality control in Medical
Microbiology Lab. Safety measures in Microbiology Laboratory, Occurrence of
labinfections, route of infections in laboratory, safety measures precaution in use of
pathogens in teaching.
Unit-II
Host pathogen interaction: Definitions - Infection, Invasion, Pathogen, Pathogenicity,
Virulence, Toxigenicity, Carriers and their types, Opportunistic
infections,Nosocomialinfections.Transmission ofinfection
Unit-III
Principle, working, use, care & maintenance of Laminar air flow, Centrifuge, Autoclave, hot
air Oven, Incubator, Colony Counter, Muffle Furnace, Mac-intos Field-
jaretc.SterilitytestingofI/vfluids,Collection,transportationandprocessingofI/v fluids for
bacterial contamination, Recording the result and interpretation
Unit-IV
Hospital acquired infection, Specimen collection from patients, clinics and hospitals,
Specimen collection for epidemiological investigations, role of microbiology laboratory in
control of nosocomial infection
Antimicrobial agents and Antibiotics: Introduction, mechanism of action, classification and

uses, Antibiotic susceptibility testing in bacteriology, Culture medium used for Antibiotic
susceptibility testing, Preparation and standardization of inoculums, Control bacterial strains,
Description, morphology, cultural characteristics, pathogenecity, cultural characteristics,
clinical features and lab diagnosis of Staphylococcus, Streptococcus, Pneumococcus,
Neisseria, Bordetella, Choice of antibiotics MIC and MBC: Concepts and methods for
determination. Various methods of Antibiotic susceptibility testing with specialreference to
Stokes and Kirby-Bauer method
Unit-V
Description, morphology, cultural characteristics, pathogenecity, cultural characteristics,
clinical features and lab diagnosis of Clostridia, Escherichia coli, Salmonella,Shigella,
Proteus, Vibrio, Pseudomonas, Spirocheates, Chlamydia, Actinomyces, Rickettsia,Yersenia,
Brucella,Description, morphology, cultural characteristics, pathogenecity, cultural
characteristics, clinical features and lab diagnosis of Vibrio, Pseudomonas, Spirocheates,
Chlamydia, Actinomyces, Rickettsia,Yersenia, Brucella,Introduction of Mycology:
Definition, general properties and classification Cutaneous mycoses, Systemic mycoses,
Opportunisticmycoses Culture and laboratory test for fungus.
BML 293: GENERAL MICROBIOLOGY-II (Practical) 3Hours/Week

JIS UNIVERSITY
1. To perform HIV Tridottest.

2. To perform radial immune-diffusiontest.
3. To perform immune-precipitation method.
4. To perform HBsAg rapidtest.
5. To perform ASO test
6. Introduction of Allergy panel
7. Mantoux test
8. Grossing of tissue
9. To perform tissue processing by manual method.
10. To perform section cutting of paraffin embedded tissue.
11. To fix the smear on glassslide.
12. To perform hematoxylin and eosinstaining.
13. ToperformPASstaining.
14. ToperformAFBstaining.
JIS UNIVERSITY
BML- 204 BIOCHEMISTRY-I (THEORY) 45HOURS
Course Content
Unit-I
Introduction to Clinical Biochemistry and role of Medical Lab Technologist , ethics,
responsibility, safety measure and hazards in clinical biochemistry lab and first aid in laboratory
accidents.
Glassware’s & plastic ware’s used in lab, calibration of volumetric apparatus, cleaning& care
and maintenance
Unit II
Principle, working, care & maintenance and calibration of Weighing balance, Hotplate, Magnetic
stirrer, Centrifuges, Incubator, Hot air oven, Colorimeter,
Spectrophotometer, Water distillation plant, Deionizers Henderson Hassel balch equation, pH
paper, pH meter, method of pH measurement
Unit-III
Preparation of solution and reagents, normal solution, molar solutions, percent solution, buffer
solution, dilutions, w/v, v/v, standard solution, aqueous solutions, concepts of acid and base
Units of measurement: SI unit, reference range, conversion factor, units for measurement of bio
metabolite, enzymes, protein, drugs, hormones, vitamins
Unit-IV
Specimen collection and processing of blood, urine & CSF, separation of serum and plasma,
deproteinization of sample, Handling of specimens for testing, preservation of specimen,
transport of specimen, factors affecting the clinical results, effect of storage onsample
Unit- V
Physical, chemical and microscopic examination of urine, Bence Jones Proteinuria and its
clinical significance, qualitative test of urine for reducing sugars, protein, ketone bodies, bile
Salt, bile pigments, urobilinogen, occult blood, uric acid, urea and Creatinine, quantitative
estimation of 24 hrs urine for protein and their clinical significance.
BML- 294 BIOCHEMISTRY-II (LAB) 3Hours/Week

1. To study general laboratory safetyrules.
2. To demonstrate glasswares,apparatus and plasticwares used inlaboratory.
3. Collection of bloodsample
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4. To separate serum andplasma.
5. Preparation of different percentagesolutions
6. Preparation of normal and molar solutions.(0.1 N NaOH, 0.2N HCl,0.1 MH2SO4)
7. Demonstration ofphotocolorimeter
8. Demonstration ofspectrophotometer
9. Demonstration of pHmeter
10. Deproteinization of bloodsample
JIS UNIVERSITY
BML-205 FUNDAMENTALS OF COMPUTER SCIENCE (THEORY) 45HOURS
Course Content
1. Introduction
2. MS Windows(Windows ’98 SecondEdition)
3. Desktop, creation of folders and shortcuts, features of Windowsexplorer
4. MS Office packages – Word, Excel, PowerPoint, basic skills in using these tools
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B.M.L.T.
2ND YEAR
JIS UNIVERSITY
SEMESTER III
BML-301 HEMATOLOGY AND BLOOD BANKING-I (THEORY) 45HOURS
Unit –I
RBCs, formation, morphology, cytoskeleton, anisocytosis, poikilocytosis, metabolism,
role of 2, 3- BPG and oxygen dissociation curve.Anaemia and its classification,
Morphological and etiological, pathogenesis, laboratory investigations and
management,Iron deficiency anaemia, metabolism of iron, pathogenesis, laboratory
investigations and management, principle and procedure of special test
Megaloblasticanaemia, pernicious anaemia, pathogenesis, laboratory investigations
Unit-II
Haemoglobin, its synthesis and types, normal and abnormal hemoglobins, extravasccular
and intravascular hemolysis.Haemolyticanaemia, pathogenesis and laboratory
investigations, principle andprocedure of special test, G-6-PD
Unit –III
Leukopoiesis, Stages of Leukocyte Maturation, Features of Cell Identification,
leucocytosis and leucocytopenia, neutrophilia, eosinophilia, basophilia, monocytosis,
lymphocytosis, neutropenia, lymphopenia, causes and significance, toxic granulation,
Morphological alterations in neutrophil, effect of HIV on blood cellparameter
Unit-IV
Overview of hemostasis and coagulation, Stages of platelets development, Primary and
Secondary hemostasis, Role of platelets, Role of coagulation factors, Coagulation
inhibitorysystem, Fibrinolysis
Unit-V
General blood picture, estimation of iron, TIBC, Transferrin, Ferritin, Plasma
haemoglobin,Vit.B12, Folic acid, FIGLU test, Schiling test, Parietal cell antibodies,G-6-
PD, Osmotic fragility test, Heinz bodies, Perls Prussian staining, Platelet count, Platelet
aggregation test, PT, INR APTT, Mixing experiments in PT and APTT, Thrombin time.
Unit- VI
Aplastic anaemia, Anaemia of chronic disorders, Sideroblasticanaemia,
HaemolyticAnaemia, etiology, pathogenesis, clinical features, laboratory
investigations,Bone marrow examination, composition & functions, aspiration
techniques, processing andstaining
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Unit-VII
Hemoglobinopathies, qualitative and quantitative, Sickle cell anaemia, sickle cell
trait,etiology, pathogenesis, clinical features, and laboratory investigations,Disease
management and prognosis, Sickling test,
Thalassaemia,classification,etiology,pathogenesis,clinicalfeatures,laboratory
investigations, haemoglobinelectrophoresis
BML-391 HEMATOLOGY AND BLOOD BANKING-I (LAB) 3HOURS/WEEK
1. General blood picture

2. Determination of red cell indices
3. Demonstration of hypochromic microcytic slide.
4. Determination of G-6-PD
5. Differential Leukocyte Count.
6. Absolute leucocyte count
7. Demonstration of toxic granulation of neutrophil
8. To perform PT and Calculate INR
9. To perform APTT
10. To perform sickling test
11. Determination of Plasma Hemoglobin
12. To perform reticulocyte count.
JIS UNIVERSITY
BML-302 CLINICAL BIOCHEMISTRY-I (THEORY) 45HOURS
Unit-I
Introduction to Clinical Biochemistry and role of Medical Lab Technologist, ethics,
responsibility,safetymeasureand hazardsinclinicalbiochemistrylabandfirstaidin
laboratoryaccidents.Glassware’s & plastic ware’s used in lab, calibration of volumetric
apparatus, cleaning& care and maintenance
Unit II
Principle, working, care & maintenance and calibration of Weighing balance, Hotplate,
Magnetic stirrer, Centrifuges, Incubator, Hot air oven, Colorimeter,Spectrophotometer,
Water distillation plant, Deionizers Henderson Hassel balch equation, pH paper, pH meter,
method of pH measurement,
Unit-III
Preparation of solution and reagents, normal solution, molar solutions, percent solution,
buffer solution, dilutions, w/v, v/v, standard solution, aqueous solutions, concepts of acid
and base. Units of measurement: SI unit, reference range, conversion factor, units for
measurement of bio metabolite, enzymes, protein, drugs, hormones, vitamins
Unit-IV
Specimen collection and processing of blood, urine & CSF, separation of serum and
plasma,deproteinizationofsample,Handlingofspecimensfortesting,preservationof specimen,
transport of specimen, factors affecting the clinical results, effect of storageonsample
Unit- V
Physical, chemical and microscopic examination of urine, Bence Jones Proteinuria and its
clinical significance, qualitative test of urine for reducing sugars, protein, ketone bodies,
bile Salt, bile pigments, urobilinogen, occult blood, uric acid, urea and Creatinine,
quantitative estimation of 24 hrs urine for protein and their clinical significance.
BML-392 CLINICAL BIOCHEMISTRY-I (LAB) 3HOURS/WEEK
1. To identify carbohydrates in given solution by various methods.

2. To determine protein by Biuret method.
3. To perform protein test by various methods.
4. Physical examination of urine
5. Urine sugar determination by Benedict’s method.
6. Protein by heat and acetic method
7. Bile salt, Bile pigments and Urobilinogen determination
8. Determination of Ketone bodies
9. Determination of various parameters of urine by uristik method.
JIS UNIVERSITY
BML- 303 BASIC AND CLINICAL PHARMACOLOGY (THEORY) 45HOURS
Unit-1
General Pharmacology: Pharmacology; Different branches of Pharmacology; Routes of drug
administration; Absorption, Distribution, Metabolism and excretion of drugs; General
mechanism of drug action; Animal used in experiments; Animal handling and ethics; Bioassay
procedures; Instruments used in Pharmacology; Basics of Clinical trials.
Unit- II
Drugs Acting on CNS: General anesthetics; Anxiolytic and hypnotic drugs; Psychotropic agents;
Epilepsy and Anticonvulsant drugs; Narcotic analgesics and antagonists; Centrally acting muscle
relaxation and anti-parkinsonism agents; Analgesics; antipyretics; anti-inflammatory agents and
Central nervous system stimulant; Local anesthetics.
Unit III
Drugs Acting on ANS: Autonomic nervous system and neurohumoral transmission;
Cholinergic or parasympathetic drugs; Anticholinergic or parasympathomimetic drugs;
BACHELOR OF MEDICAL LAB. TECHNOLOGY-BMLT Adrenergic or sympathomimetic
drugs; sympatholytic drugs; Drugs acting on autonomic ganglion; Neuromuscular blockers.
Unit IV
Drugs Acting on Respiratory System: Bronchodilators; analeptics; Nasal decongestants,
expectorants; antitussive agents.
Unit V
Drugs acting on Cardiovascular System: Antiarrhythmic drugs; Cardiotonics; Antianginal drugs;
Antihypertensive drugs; Drugs used in atherosclerosis.
Unit VI
Drugs Acting on Blood and Blood Forming Organs: Haematinics – Iron (Fe); Coagulants;
Anticoagulants; Blood and plasma expanders.
Unit VII
Hormones and Hormone Antagonists: Antithyroid drugs; Hypoglycaemic agents; Sex hormones
and oral contraceptives; Corticosteroids.
Unit VIII
JIS UNIVERSITY
Opioid Analgesics: Endogenous opioid peptides; Opioid receptors; Effects of clinically used
opioids; Morphine and related opioid agonists; Acute opioid toxicity; Opioid agonist &
antagonist; Therapeutic uses of opioid analgesics.
Unit IX
Drug Addiction and Drug Abuse: Drug dependence; Physical dependence on Drugs;
JIS UNIVERSITY
BML- 304 HEALTH EDUCATION & HEALTH COMMUNICATION(THEORY)

45HOURS
Unit I:
• Health Education: Principles & Objectives, Levels of Health Education,
Educational Methods, Evaluation & Practice of Health Education inIndia.
• Health Counseling: Introduction, Theories, Process &Techniques.
• Health Care Reporting, Role of NIC & Other Bodies, Research in Health Education
Unit II:
• Heath Communication: Basic Concept & Principles of Communication,
Definition, Purpose, Types ofCommunication
• Communication Process, Directions of Communication: Upward, Downward,
Lateral, Factors influencing Communication, Barriers of Effective Communication,
How to overcome theBarriers
• Models of communication: Aristotle Model, Shannon and Weaver model,
Schramm Model, Laegans Model, Fano Model, Literer’s Model, Westly
Maclean’sModel.
Unit III:
Mass communication & Role of Media in healtheducation

• Information Communication Technologies (ICT) in health care and awareness.
(Telemedicine & e-health, communityradio)
• Future trends in information and communications systems:
JIS UNIVERSITY
BML-305 BIOMEDICAL WASTE MANAGEMENT (THEORY) 45HOURS
Unit 1: Present Scenario

Bio-medical waste – Concepts and Perceptions, Waste Generation, Segregation ,
Disposal
Unit 2:
Planning and Objectives of BMW Management, Survey, Policies and
Perspectives of BMW Management
Unit 3:
Record Keeping, Management of Bio-medical Waste, Technologies for Treatment
for BMW, Criteria for selecting appropriate Medical Waste Technologies
Unit 4:
Training, Occupational Safety and Health Issues
Unit 5:
Legal Aspects and Environment Concern, Implementation of
Action Plan, Approaches to Common Regional facility
JIS UNIVERSITY
SEMESTER IV
BML-401 HEMATOLOGY AND BLOOD BANKING-II(THEORY) 45HOURS
Unit-I
Leukemia and its classification, WHO and FAB classification, AML, ALL,CML, CLL, its
etiology, clinical features, laboratory investigations Cytochemistry involved in diagnosis
of various types of leukemia.
Unit-II
Qualitative and quantitative disorders of platelets, hypercoaguable test, Disorders of
secondary hemostasis, hemophilia and its lab diagnosis,Von- Willebrand disease,
Disseminated intravascular coagulation, thrombosis, Disorder of fibrinogen, test for
bleeding & coagulation disorders,correction, studies for factor deficiency, quantitative
factor assay
Unit- III
LE cells, its demonstration and significance, lupus anticoagulants, Blood parasites,
Malaria, Trypanosomes, Filariasis, Leishmania
Unit-IV
Basic Principles of Blood Banking, Antigen, Antibody, naturally occurring antibody,
Complement, ABO & Rh blood group system, Methods of blood group determination,
Forward and Reverse grouping, Slide & Tube method, Gel method.
Unit-V
Other blood group system such as Lewis, MNS, Kell Duffy etc. Anticoagulants and
preservative used in blood bank, Donor selection criteria, Blood collection and processing
Unit-VI
Transfusion transmissible infectious disease screen, Coomb’test, Cross matching,
Compatibility testing, Antibody Screening & Identification, Grading of
Reaction/Agglutination
Unit-VII
Blood components and its preparation, preservation, storage and transportation.
Indicationsfordifferentbloodcomponenttransfusion,Bloodtransfusionreactionanditstype,
HDN Introduction of stem cell banking and bone marrowtransplantation.
Unit-VIII
Apheresis, indications of hemapheresis, plasmapheresis, plateletspheresis, plasmapheresis
Quality control of reagents, equipments, bloodcomponents used in transfusion medicine.
Role of NACO, Indian Red Cross Society, DGHS and blood transfusionservices.
JIS UNIVERSITY
BML-491 HEMATOLOGY AND BLOOD BANKING-II (LAB) 3HOURS/WEEK

1. Stainingofbonemarrow
2. Toperformsicklingtest.
3. To determine fetal haemoglobin
4. To perform Heinzbodies
5. Demonstration of leukemicslides
6. To perform LAPscoring
7. To determine total plateletcount
8. To performPT
9. To performAPTT
10. To perform thrombintime.
11. To perform D-dimertest.
12. To determine fibrinogenconc.
13. General bloodPicture
14. Todemonstratemalarialslide
15. Haemoglobinelectrophoresis
16. Demonstration of hemoparasites like trypanosomes, Filaria,Malaria
JIS UNIVERSITY
BML-402 CLINICAL BIOCHEMISTRY- II(THEORY) 45HOURS
Unit-I
Introduction to enzymes, Classification of Enzymes, Isoenzymes, Concept of lock and key
and induced fit theory, concept of activation energy and binding energy. Factors affecting
enzyme activity
Unit-II
Coenzyme: Classification, various types and function, structure of NAD+, NADP+, FAD
and FMN, PPP. Units for measuring enzyme activity, factors affecting enzyme
levelinserum/plasma. Clinicalassay&itstype, kineticassayand end point assayfor theenzymes
Unit-III
Enzyme kinetics, the Michaelis-Menten equation and its physiological significances,
Enzyme Inhibition, types of inhibitors of enzyme
Unit-IV
Isoenzymes,theirtissuedistributionandclinicalsignificance:ALT,AST,ALP,GGT,CPK,CK-
MB, LDH,Troponin, Myoglobin, Amylase, Lipase,ACP
Unit-V
BasicConceptsofAutomation,principle,workingandmaintenanceofvariousclinical chemistry
analyzers, point of care testing, Hospital LaboratoryManagement
BML-492 -CLINICAL BIOCHEMISTRY-II (LAB) 3HOURS/WEEK
1. Preparation ofhemolysate
2. To determine T3conc. in serumsample.
3. To determine T4conc. in serumsample.
4. To determine TSH conc. in serumsample.
5. To determine LH conc. in serumsample.
6. To determine FSH conc. in serumsample.
7. To determine Prolactin conc. in serumsample.
8. To determine TSH conc. in serumsample.
9. To perform TRIPLEtest.
10. Demonstration of male and female infertilitytest.
11. BetaHCG
JIS UNIVERSITY
BML-403 BACTERIOLOGY, IMMUNOLOGY AND PARASITOLOGY(THEORY)

45HOURS
Unit-I
Historical background, general concepts of the immune system, innate and adaptive
immunity; active and passive immunity; primary and secondary immune response.Cell and
organs of immune system, Phagocytosis.
Unit-II
Antigens and haptens: Properties,foreignness, molecular size,
heterogeneity,BandTcellepitopes;TdependentandT independent antigens.Antibodies:
Historical perspective of antibody structure; structure, function and properties of the
antibodies; different classes, subclasses and biological activities of antibodies; concepts of
antibodydiversity, isotype, allotype,Introduction of hybridoma technology, monoclonal
antibodies, polyclonal antibody
Unit-III
Mechanism of humoral and cell mediated immune response.Introduction of Major
Histocompatibility Complex, organization of MHC and inheritance in humans; Antigen
presenting cells, antigen processing and presentation, Complement system and complement
fixation test.
Unit-IV
Laboratory tests for demonstration of antigen – antibody reaction such as agglutination,
precipitation, ELISA, RIA, Immunofluorescence,
Unit-V
Rheumatological diseases, etiology and pathogenesis and lab investigations
Unit-VI
Introduction of parasites, host, zoonosis, host parasites relationship, sources of infection,
mode of infection, pathogenesis, immunity in parasitic infection
Lab diagnosis- Entamoebahistolytica, Malarial Parasites, Leishmania, Trypanosomes, their

morphology, life cycle,pathogenesis,clinical features and lab diagnosis.
Helminthology: Introduction and classification, Taeniasolium, TaeniaSaginata,Fasciola,

Ascaris, Wuchereriabancrofti their morphology, life cycle, pathogenesis, clinical features
and lab diagnosis.Hookworm, Trichuris. Dracunculus their morphology, life cycle,
pathogenesis, clinical features and labdiagnosis
Unit-VII
Diagnostic methods in Parasitology: Introduction, Examination of stool, urine, blood,
Culture methods, Immunological diagnosis andserology
JIS UNIVERSITY
BML-493 BACTERIOLOGY, IMMUNOLOGY AND PARASITOLOGY (LAB)

3HOURS/WEEK
1. Demonstration of Microscope and itsparts

2. Demonstration of glassware used inmicrobiology.
3. Demonstration of autoclave and sterilization of glasswares.
4. Demonstration of Hot air oven and sterilization of glasswares.
5. To perform Gramstaining
6. To perform Acid fast staining (ZeihlNeelsenstaining)
7. To perform Indian ink staining
8. To perform Hanging dropmethod
9. Demonstration ofcapsule
10. Staining of bacterialspores
11. To demonstrate agglutinationreaction.
12. To perform RAtest
13. To perform WIDALtest
14. ToperformRPRtest.
15. ToperformCRPtest.
JIS UNIVERSITY
BML-404 COMMUNITY MEDICINE(THEORY) 45HOURS
Unit I
Natural History of Disease: Determinants of health, multi – factorial causation of disease host,
agent, and environment relationship primary, secondary and tertiary levels of prevention with
examples related to few diseases of national importance.
Unit II
Mode of Transmission of Disease: Air – borne, vector and vehicle transmission; Methods of
control with examples for control of each mode.
Unit III
Disinfection: Disinfection of the infective materials received in the Laboratory by using the
appropriate disinfection methods, at the health centre level.
Unit IV
Health Services: Brief description of organization of health services at the centre and state levels;
Primary Health Care - Definition, components and principles of primary health care; Health for
all indicators; Primary Health Centre - The functions, staffing pattern and the role of laboratory
technicians in primary Health Centre.
Unit V
National Programmes of Health and Disease Eradication /Control: Health Programmes Family
Welfare Programme, National Programme for water supply and sanitation, Nutritional
Programmes, Immunization and universal immunization programme; Disease Eradication
programme - Leprosy &Guniea worm; Disease control programmes - Tuberculosis, Malaria,
Filaria, S.T.D, Goitre, Cholera and other diarrhaeal diseases and National Programme for
prevention of blindness including trachoma.
Unit VI
Demography & Population Control: The factors influencing population growth, death rate, birth
rate and methods of contraception.
Unit VII
Biostatistics: Application of statistical principles in history; Presentation of data, calculation of
mean, median and mode, range and standard deviation and their significance; Significance of ‘T’
test, Chi square values.
JIS UNIVERSITY
Unit VIII
Environmental Sanitation: Methods of water purification and disinfection, collection of water
samples, their transport and bacteriological analysis; Methods of excreta disposal.
Unit VIII
Health Education: Definition, principles, objectives, purpose, types and AV aids;
Communication - definition, process and types, Behavioral change communication; IEC
(Information education and communication) - aims, scope, concept and approaches; Inter
personal relationship - Co-ordination and co-operation in health education with other members of
the health team; Teaching and learning process, concept, characteristics of leaner and educator;
Role and skill of health professional in Health Education
JIS UNIVERSITY
B.M.L.T.
3RD YEAR
JIS UNIVERSITY
SEMESTER V
BML-501 VIROLOGY, MYCOLOGY AND APPLIED MICROBIOLOGY-I (THEORY)

45HOURS
Unit- I
Western blotting, Immunodiffusion, Immunoelectrophoresis, Hypersensitivity and itstypes
Introduction to Allergy and its laboratory test
Unit-II
Introduction of transplant immunology, graft rejection, tissue typing for kidney and bone
marrow transplant, Laboratory test for transplant
Unit –III
Autoimmune disorders, pathogenesis, organ specific and systemic autoimmune disorders
and its markers such parietal cell antibody, anti-sperm antibody, lupus anticoagulants, anti-
mitochondrial antibody, ANA, ds DNA, HLA-B27, ASMA, anti
CCP
Unit-IV
Immunological disorders: primary and secondary immunodeficiency, SCID,
AIDS,Tumour, types of tumours, Various Tumour Markers, their significance and method
of estimation.
Unit-V
Vaccines, classification and applications, Active and passive
immunization,Immunoprophylaxis schedule in neonates, children and in pregnancy
Unit VI
Nature and Properties of Viruses, Introduction: Discovery of viruses, nature and definition
of viruses, general properties, concept of viroids, virusoids, satellite viruses and Prions.
Structure of Viruses: Capsid symmetry, enveloped and non-enveloped viruses
Isolation, purification and cultivation of viruses, Viral taxonomy: Classification and

nomenclature of different groups of viruses, Modes of viral transmission: Persistent, non-
persistent, vertical and horizontal. Viral multiplication and replication strategies:
Interaction of viruses with cellular receptors and entry of viruses. Assembly, maturation
and release of virions
Unit- VII
Poxviruses, Herpesviruses, hepaptitis viruses, retroviruses-HIV, Picorna viruses,
rhabdoviruses, orthomyxoviruses and paramyxo viruses, TORCH profile,Symptoms, mode
of transmission, prophylaxis and control of Polio, Herpes, Hepatitis, Rabies, Dengue, HIV,
Influenza with brief description of swine flu, Ebola, Chikungunya, Japanese Encephalitis
Unit VIII
Introduction to oncogenic viruses, Types of oncogenic DNA and RNA viruses, concepts of
JIS UNIVERSITY
oncogenes and proto-oncogenes, prevention & control of viraldiseases, antiviral

compounds and their mode of action, interferon and their mode of action,
Generalprinciplesofviral vaccination
BML-591 VIROLOGY, MYCOLOGY AND APPLIED MICROBIOLOGY-I (LAB)

3HOURS/WEEK
1. Leishman staining for malarialparasites

2. DemonstrationofpermanentslideofTrichuris,AscarisandHookworm
3. Salinewetmountforobservingovaandeggsofparasites.
4. Iodinewetmountforobservingovaandeggsofparasites.
5. Concentration of stool samples byfloatationmethod
6. Zinc sulphate conc. Method for stoolsample
7. Demonstrationofvariousparasitesbypermanentslides.
8. Concentrationofstoolsamplebysedimentationmethod
9. SerologicaldiagnosisofLeishmania
10. AldehydeChopratestforKalaAzar
11. To perform HBsAg/ Australia Ag by rapidmethod
12. To perform HBsAgbyELISA
13. To perform HIV Tridotmethod.
14. To perform HIV byELISA
15. To perform TORCHprofile
16. Demonstration of PCRHBV
17. Demonstration of PCR HIV Viralload
JIS UNIVERSITY
BML-502 HISTOPATHOLOGY AND CYTOPATHOLOGY TECHNIQUE (THEORY)

45HOURS
Unit-I
Staining of carbohydrates: preparation of Schiff reagent, PAS staining, Alcian blue,
staining of glycogen, Amyloid, other staining method. Connective tissue & its staining:
Trichrome staining, verhoeff stain, WeigertResorcin stain, Gordon’s and Sweet stain,
Gomori’s method, von Geison stain,PTAH stain
Unit-II
Demonstration of minerals and pigments in tissue sample, Demonstration and
identification of lipids, Demonstration of enzymes, diagnostic application and the
demonstration of phosphatases, dehydrogenases, oxidases and
peroxidases,Demonstrationofmicroorganismontissuespecimens,Bacteria,AFB,
Actinomyces, spirochetes, fungi
Unit-III
Demonstration of nucleic acids, Processing and staining of bone marrow sample. Fixation,
Processing and section cutting of bones, eye ball, Techniques in neuropathology: Neurons
staining, Myelin, Neuropathology lab specimenhandling
Unit-IV
Demonstration of sex chromatin, Museum techniques, Electron microscopy: Principle and
working, fixation, processing and staining of tissue Fluorescence Microscope: Principle
and working
Unit- V
Immunohistochemistry: principle, types, applications, antigen retrieval, APAAP,PAP
Staining, Quality control in histopathology
Unit-VI
Microtome, its type and working, various type of microtome,Microtome knives, itstype
and knife sharpening,Section cutting, fault and remedies,Section adhesive
Unit-VII
Cryostat, frozen sections of fresh, fixed and unfixed tissue, freeze drying, rapid frozen
sections and staining for emergencydiagnosis, Dye chemistry, Stains and dyes, natural
dye, acidic dye, basic dye, neutral dyes, fluorescence dye, mordant, accelerators,
accentuators, metachromasia, metachromatic dyes
Unit- VIII
Progressive, regressive, vital, supravital staining, types of hematoxylin,Haematoxylin and
eosin staining, use of control sections in tissue staining, mounting and mounting media,
advantages & disadvantages, refractive index
JIS UNIVERSITY
BML-592 HISTOPATHOLOGY AND CYTOPATHOLOGY TECHNIQUES (LAB)

3HOURS/WEEK
1. Demonstration of glass wares and equipment used in histopathologylab.

2. To prepare alcohol of differentconcentration.
3. To prepare formalin from stocksolution.
4. To sharp knife by honing andstropping.
5. Grossing oftissue
6. To perform tissue processing by manualmethod.
7. To perform section cutting of paraffin embeddedtissue.
8. To fix the smear on glassslide.
9. To perform hematoxylin and eosinstaining.
10. Preparation of various cytologicalfixatives
11. Preparation of various stains used incytology
12. Preparation ofsmear
13. To perform PAPstaining
14. To perform Giemsa staining on fluidsample
15. To prepare cellsuspension
16. Processing of various fluidsamples
JIS UNIVERSITY
BML-503 HISTOTECHNOLOGY (THEORY) 45HOURS
Unit-I
Cell: basic structure and function, cell organelles, cell cycle, Benign and Malignant tumors,
Instrumentsusedincytology,preparationofbuffers,stains, Microscopy:Light, compound, phase
contrast,fluorescence
Unit-II
Introduction of histopathology, cytology &histotechniques, laboratory
organization,care&maintenanceofequipmentsusedinhistotechnologylab, Safety measures in
histotechnology lab Reception, Recording, Labelling and transportation of tissue
specimens,Basic concepts of fixation and varioustypesof fixative used in histopathology and
cytopathology
Unit-III
Tissue and its types, Location and function,Grossing of tissues, whole mount,
sections,smears,tissueprocessinganditssteps,manualandautomatedmethod, components &
principle of automatic tissueprocessor. Decalcification, decalcification methods, types of
decalcifying fluid, Processing of bones and teeth, Embedding media, its type and properties
Unit- IV
Instruments and equipments used in cytology Fixation and Fixatives used in cytology,
Adhesive and mounting media, Cell block and cytospin technique,Staining such as PAP,
Diff-quick, MGG, H&E, Shorr staining, significance of PAP-HPV, Destaining and
restaining of slides, Cover slipping
Unit-V
Aspiration and exfoliative cytology, Patient preparation, Sample collection, Fixation,
Processing and Staining FNAC, collection, processing of sample and staining, on site quick
staining procedure
Unit-VI
Pap staining, Progressive & Regressive, Hormonal cytology in different
agegroups,Collection and processing of sputum, BAL, CSF, Pleural, peritoneal and
pericardial fluid, Gynaecologic sample
Unit-VII
Sex chromatin demonstration, Introduction of Immunocytochemistry, differentmarkers and
its applications, Automation in cytology, Liquid based preparation & automated screening
device
JIS UNIVERSITY
BML-504 CLINICAL ENZYMOLOGY & AUTOMATION (THEORY) 45HOURS
Unit-I
Introduction to enzymes, Classification of Enzymes, Isoenzymes, Concept of
lock and key and induced fit theory, concept of activation energy and binding
energy. Factors affecting enzyme activity
Unit-II
Coenzyme: Classification, various types and function, structure of NAD+,
NADP+, FAD and FMN, PPP. Units for measuring enzyme activity, factors
affecting enzyme levelinserum/plasma. Clinicalassay&itstype,
kineticassayand end point assayfor theenzymes
Unit-III
Enzyme kinetics, the Michaelis-Menten equation and its physiological
significances, Enzyme Inhibition, types of inhibitors of enzyme
Unit-IV
Isoenzymes,theirtissuedistributionandclinicalsignificance:ALT,AST,ALP,GGT,CPK,CK-
MB, LDH,Troponin, Myoglobin, Amylase, Lipase,ACP
Unit-V
BasicConceptsofAutomation,principle,workingandmaintenanceofvariousclinical
chemistry analyzers, point of care testing, Hospital LaboratoryManagement
JIS UNIVERSITY
SEMESTER VI
BML-602: ESSENTIALS OF MEDICAL PHARMACOLOGY(THEORY) 45HOURS
Unit-I
Hormones, Classification of hormones, organs of endocrine system their secretion and
function, regulation of hormone secretion, Mechanism of action
Unit-II
Thyroid function test: Thyroid hormones, biological function, hypothyroidism,
hyperthyroidism, Determination of T3, T4, TSH, FT3, FT4, TBG, Disorder associated with
thyroid dysfunction.
Unit-III
Infertility profile: LH, FSH, TSH, Estrogen, Progesterone, Total
Testosterone,Freetestosterone,DHEA-S,17-Ketosteroids,Prolactin, their estimation and
clinical significance, reference range, hypo and hyper secretion, TripleTest
Unit-IV
Growth hormone, ACTH, Aldosterone, Cortisol their estimation and clinical significance,
reference range, hypo and hyper secretion
Unit-V
Introduction of Toxicology, Alcohol poisoning, Lead poisoning, Zinc poisoning, Mercury
poisoning drugs abuse, screening procedure for drug screening, Spot tests, hair and urine
test, Immunoassay for drugs.
JIS UNIVERSITY
BML-603: BIOMEDICAL TECHNIQUES, LAB MANAGEMENT AND

ETHICS(THEORY) 45HOURS
Unit-I
Chromatography, its principle, types and applications.Paper Chromatography, Thin layer
chromatography, HPLC, Gas liquid chromatography, Ion exchange chromatography and
their application in diagnosis.
Unit-II
Basic Principle of electrophoresis, Paper electrophoresis, Gel electrophoresis, PAGE, SDS-
PAGE, Agarose gel electrophoresis, buffer systems in electrophoresis.Electrophoresis of
proteins and nucleic acids, haemoglobin, immunoglobulin’s, isoenzymes Applications of
electrophoresis in clinical diagnosis.
Unit-III
Centrifugation, fixed angle and swinging bucket rotors, RCF and sedimentation coefficient,
differential centrifugation, density gradient centrifugation andUltracentrifugation.
Unit-IV
Radioisotopes, Radioactivity, instruments for radioactivity measurement, applications of
radioisotopes in clinical biochemistry
Unit-V
Immunoassay: ELISA, RIA, FIA, FACS and their applications in clinical diagnosis.
Unit-VI
Ethical Principles and standards for a clinical laboratory professional duty to the patient,
duty to colleagues and other professionals, Good Laboratory Practice (GLP)
,Introduction to Basics of GLP and Accreditation, Aims of GLP and Accreditation,
Advantages of Accreditation, Brief knowledge aboutNational and International Agencies
for clinical laboratory accreditation
Unit-VII
Awareness/Safety in a clinical laboratory, General safety precautions.HIV: pre- and
post-exposure guidelines, Hepatitis B & C: pre- and post- exposure guidelines, Drug
Resistant Tuberculosis. Patient management for clinical samples collection,
transportation and preservation, Sample accountability, Purpose of accountability,
Methods of accountability
Unit-VIII
Sample analysis: Introduction, factors affecting sample analysis, reporting results, basic
format of a test report, reported reference range, clinical alerts, abnormal results, results
from referral laboratories, release of examinationresults, alteration in reports
JIS UNIVERSITY
Unit-IX
Quality Management system: Introduction, Quality assurance, Quality control system,
Internal and External quality control, quality control chart, BiomedicalIntroduction and
importance of calibration and Validation of Clinical Laboratory instrument,
EthicsinMedicallaboratoryPractice,EthicsinrelationtoPre-Examinationprocedures,
Examination procedures, reporting of results, preserving medicalrecords, Procurement of
equipment and Inventory Control,
Unit-X
Audit in a Medical Laboratory, Introduction and Importance, NABL &
CAP,Responsibility, Planning, Horizontal, Vertical and Test audit, Frequency of audit,
Documentation
BML- 601 DIAGNOSTIC MOLECULAR BIOLOGY(THEORY) 45HOURS

JIS UNIVERSITY
Unit I
Basic transcription apparatus, Initiation, elongation and termination of
transcription, Eukaryotic Transcription of mRNA, tRNA and rRNA, types of
RNA polymerases, transcription factors Introduction of translation
Unit-II
Nucleicacidamplificationtesting,PCR,Principle,Types,applications,Thermal
cycler,RTPCR, reversetranscriptasePCR, NestedPCR
Unit-III
Blotting techniques, southern blotting and Western blotting
Introduction to chromosomes, its structure and disorder, Karyotyping, Chromosomal
studies in hematological disorders (PBLC and Bone marrow), FISH
Unit-IV
Radioisotopes and its application in measurement of blood volume,
determinationofredcellvolumeandplasmavolume,redcelllifespan,platelet
lifespan,radiationhazardsanditspreventiondisposalof radioactivematerial
Introduction and applications of Flow cytometry, Stem cell banking, Prenatal
Diagnosis
Unit-V
Nucleic Acids, DNA, RNA, composition, structure, types, denaturation and
renaturation of DNA, chemistry of DNA synthesis, general principles of
replication, enzyme involved in DNA replication
– DNA polymerases, DNA ligase, primase, telomerase and other accessory proteins.
BML691 -DIAGNOSTIC MOLECULAR BIOLOGY(LAB) 3HOURS/WEEK
1. Isolation ofDNA
2. Separation of DNA by Agarosegelelectrophoresis
3. Demonstration of thermal cycler andPCR.
4. HIV test by WesternBlotting
5. To performkaryotyping
6. Demonstration of PCR HLAB-27
7. Demonstration of PCRHIV
8. Demonstration of PCRMTB
JIS UNIVERSITY
BML-692 : HOSPITAL INTERNSHIP AND PROJECT
Students shall be deputed to various labs of Pathology department wherein they shall undergo
practical training of handling patients, collection and processing of blood, urine, sputum stool
and body fluids samples.
Identification of patient’s particulars based on CR number, Lab Number and transfer of samples
from collection centres to different labs. Process of performing various tests in different labs.
Each student is required to maintain a logbook of the various posting.
Student’s performance shall be evaluated on continuous basis by the faculty posted in various
sections. The faculty shall submit the assessment records of each student posted in his/her
section on monthly basis to the HOD. Marks will be awarded out of 100.

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JIS UNIVERSITY

[Syllabus for Bachelor of Medical Laboratory Technology (BMLT) Course]

SYLLABUS FOR THE

81, Nilgunj Road, Jagarata Pally, Deshpriya Nagar, Agarpara, Kolkata-

Duration: 3 Years (6 semesters)

B.M.L.T. or Bachelor in Medical Laboratory Technology is an undergraduate Medical

Bachelor in Medical Laboratory Technology Eligibility

Bachelor in Medical Laboratory Technology Course Suitability

How is Bachelor in Medical Laboratory Technology Course Beneficial?

Bachelor in Medical Laboratory Technology Employment Areas

Bachelor in Medical Laboratory Technology Job Types

Junior Technical Executive

Advance Courses in Bachelor in Medical Laboratory Technology

Post Graduate Diploma in Medical Laboratory Technology

M.Sc. (Medical Lab Technology)

8 BML-193 General Microbiology-I (Practical) - - 3 2

11 BML-182 Seminar and GD - - - 1

Course Period Credit

1 BML-201 Human Anatomy and Physiology- II 3 - - 3

3 BML-203 General Microbiology-II (Theory) 3 - - 3

4 BML-204 Biochemistry-II (Theory) 3 - - 3

8 BML-293 General Microbiology-II (Practical) - - 3 2

9 BML-294 Biochemistry-II (Practical) - - 3 2

11 BML-282 Seminar and GD - - - 1

10 BML-382 Seminar and GD - - - 1

9 BML-482 Seminar and GD - - - 1

Total 18+2 =20

Course Period Credit

3 BML-503 Histotechnology (Theory) 3 - - 3

4 BML-504 Clinical enzymology and automation (Theory) 3 - - 3

BML-591 Virology, Mycology and Applied Microbiology

`10 BML-582 Seminar and GD - - - 1

1 Diagonostic Molecular Biology (Theory) 3 - - 3

3 BML-603 Biomedical Techniques, Lab Management and Ethics 3 - - 3

5 BML-692 Hospital Training - - - 6

SYLLABUS FOR BACHELOR OF MEDICAL LABORATORY

BML 101: HUMAN ANATOMY AND PHYSIOLOGY-I (THEORY) 45 Hours

BML 191: HUMAN ANATOMY AND PHYSIOLOGY-I (Practical) 3 Hours/week

1. Demonstration of Major organs through models and permanentslides.

Recommended Books (Latest Editions)

BML 102: PATHOLOGY I (Theory) 45 Hours

BML 102: PATHOLOGY-I (Practical) 3 Hours/week

Recommended Books (Latest Editions)

BML 103: GENERAL MICROBIOLOGY-I (Theory) 45 Hours

BML 193: GENERAL MICROBIOLOGY-I (Practical) 3 Hours/week

1. Demonstration of Autoclave and sterilization ofmedia

Recommended Books (Latest Editions)

BML 104: BIOCHEMISTRY-1 (Theory) 45 Hours

BML 194: BIOCHEMISTRY-I (Practical) 3 Hours/week

1. To determine protein by Biuretmethod.

Recommended Books (Latest Editions)

BML 195: COMMUNICATION FOR PROFESSIONALS (Theory) 45 Hours

1. Introduction: Meaning of Communication; Role of Communication in Business; Basic

Recommended Books (Latest Editions)

BML 201: HUMAN ANATOMY AND PHYSIOLOGY-II (THEORY) 45 Hours

Endocrine system: Different hormones in endocrine system. Action of pituitary, thyroid,

Reproductive system: Male and female reproductive organs, Gametogenesis, Ovulation,

BML 291 HUMAN ANATOMY & PHYSIOLOGY-II (Practical)

1. Demonstration of digestivesystem from models.