Label-free interaction analysis

Biacore™
SPR systems
Visualize your research.
For more information and faster decisions.
View molecular
interactions in real-time
Biological processes are “real-time” events, driven and regulated by dynamic interactions
between key molecules.
End-point techniques such as ELISA offer a snapshot view of interactions. They only provide
basic information such as overall binding strength (affinity). The affinity depends on the ratio
of molecules on- and off-rates, which are time bound constants. Equal affinity interactions
can have very different kinetic properties, resulting in different biological responses. These
differences can’t be resolved by viewing a point in time.
Biacore™ systems use surface plasmon resonance (SPR), a label-free and biophysical technology.
SPR monitors molecular interactions in real-time so you can differentiate between variances in
kinetic properties, even for interactions with challenging targets.
Biacore™ systems are designed to help you to generate high quality, information-rich data, that
helps answer key questions concerning the nature of binding — driving your project to conclusion:

1. How strong? 3. How much?

Affinity is a measure of the strength of Qualitative and quantitative determination
binding/attraction between molecules. of active analyte binding to a target
Affinity is a time independent measurement protein vs total protein concentration
and is determined by measuring the binding obtained via classic absorbance
response at steady state (constant signal). measurement at 280 nm wavelength.

2. How fast? 4. How specific?

Binding kinetics determine how fast/slow Is the molecule specific for its target?

Biacore™ SPR systems

a complex forms or dissociates within a Does the antibody recognize multiple
given time period and allow the calculation derivatives? The flexibility in Biacore™
of association and dissociation rate assay design allows rapid assessment of
constants. cross-reactivity and specificity.

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Ligand — analyte binding. In real-time.

n
ti o

Dis
ci a

soc
Ass o

i at
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io

Biacore™ SPR systems

Association Dissociation The binding (association)
Interactants in solution bind to Binders allowed to dissociate from and dissociation
molecules on sensor chip surface. molecule on sensor chip surface. of molecules to the sensor chip
surface are monitored in real-time
and displayed in a sensorgram.
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Greater insights into your
interactions, irrespective Biacore™ systems

of molecule size or purity Molecules

Antibodies and antibody variants

Methods

Affinity
Fields

Basic research

Biacore™ systems provide valuable information from high-quality molecular interaction Proteins including multidomain Biotherapeutics discovery and
Kinetics
proteins development
data for a range of applications including: biological research, biotherapeutics,
small-molecule drug discovery and development, immunogenicity studies, vaccine
development and quality control. The sensitivity, performance, and robustness of Peptides Screening Small-molecule discovery
Biacore™ SPR systems enable detailed characterization of a broad range of molecular
interactions — even for challenging targets such as membrane proteins. Biotherapeutics manufacturing
Compounds and fragments Specificity
and quality control
Biacore™ systems allow you to:
Vaccine design and
• Understand the relationship between molecule structure and function. Nucleic acids Concentration
development
• Identify hits from screening campaigns and characterize leads
• Screen and characterize antibodies and proteins based on yes/no binding, affinity, Viruses Epitope binning Diagnostic kit development
and kinetics. From the fastest on-rates to the slowest off-rates.
• Quantify protein by measuring the concentration of active protein with retained Aptamers Immunogenicity
biological function.
• Validate results from other techniques/approaches such as ELISA, HTS, or other G protein-coupled receptors
Thermodynamics
(GPCRs)
orthogonal biophysical methods.

Biacore™ SPR systems

Membrane proteins Competition assays

Potency

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Streamlined and high quality SPR data
in key applications

Compounds Proteins DNA/RNA Antibody Antibodies Virus Cells

and and fragments
fragments enzymes

50 Da
50 kDa
150 kDa
~50 mDa

Understanding the nature of interactions between molecules is fundamentally important

for increased understanding of biological processes.

Biacore™ SPR systems

Biacore™ surface plasmon resonance (SPR)-based sensors are used to study a wide range
of molecular interactions in real-time and are applied as powerful tools in research and
drug discovery throughout, quality control, and drug-release testing.

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From research to discovery, development and QC
Research Drug discovery Drug development and manufacturing
Characterization of Structural/functional Lead Critical quality
Screening Characterization Potency Concentration
molecular interactions studies optimization attributes (CQA)

Visualize and streamline your research Compress and maximize your screening workflow Challenge analytical complexity
• Real-time label-free monitoring of binding events for better • Screen, select and optimize hits based on selectivity, yes/no binding, • Fully characterize ligand-target binding for in-depth understanding

Biacore™ SPR systems

understanding of molecular interactions. affinity, and kinetics. of critical quality attributes (CQAs)
• K
 inetics, affinity, transition state thermodynamics, and yes/no • Design your drug candidates with desired target binding profile. • Measure product concentrations using regulatory recommended
binding data help you validate your hypotheses, understand SPR technology
the mechanism of action, and make meaningful conclusions. • A
 nalyze dual-target specificities of bispecific antibodies in a single
assay setup.

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SPR made simpler
and faster
Cytiva has developed a range of tools designed specifically to make Biacore™ assays as
easy and reliable as possible, suitable for users of all levels of experience. The complete
toolbox is backed by stringent development, production methods and quality control.
Several Biacore™ systems facilitates compliance with worldwide regulatory expectations
by offering validation services, 21 CFR Part 11 functionality and electronic signatures.

Get started quickly at any experience level

Predefined methods with application relevant parameters, combined with an intuitive
easy to use interface, empowers you to design your experiments quickly.
Biacore™ Insight Software is the complete instrument control and data evaluation
software for Biacore™ 1 series and Biacore™ 8 series systems. Optimized solutions for
common applications are offered via add-on software extensions and provide additional
functionality and tools to further streamline your analysis and reduce time to results.
The modular design of the software allows you to maximize the application versatility of
your Biacore™ systems, providing a scalable, easy to use, platform that grows with your
evolving needs. Quality control tools help you to assess high-quality kinetic and affinity
data for improved interpretation of results.
Our SPR machine learning software, Biacore Intelligent Analysis™, supports you to reduce
the bottleneck even further and rapidly analyze large data sets.

Ready-to-go interaction analysis

Our capture kits significantly reduce assay development time, giving consistent capture

Biacore™ SPR systems

of antibodies and molecules via the most common tags. Ready-made buffers and
solutions are available for your convenience, ease of use, and to ensure reliable results.
Biacore™ sensor chips offer support for analysis of a wide range of interactions, backed
up with a selection guide to help you select and use the right tools for the application.

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Together in research
Biacore™ service and support
Cytiva is committed to supporting customers with innovative tools, analytics, and
everyday lab reagents to uncover new insights in research or to discover, develop and
manufacture drugs.

More than 60 000 scientific publications and still counting...

Thousands of Biacore™ systems are installed globally and more than 60 000 scientific
articles have been published in peer-reviewed journals where Biacore™ systems have
been featured for a wide range of applications.

Experienced application specialists and service experts

Our instrument service and application support are performed by specially trained
service experts available close to you. They can help you to get the most out of your
Biacore™ system for all applications and improve efficiency by minimizing your
equipment system downtime. This allows you to focus on your work delivering reliable
binding analysis results.
To learn more about assay setup and data interpretation, Cytiva offers a wide range of
self-training tools, classroom trainings, handbooks, and lab protocols.

Join our family — Biacore™ community

All Biacore™ users are invited to share their experiences and learn more at regional user
days. Developments in Protein Interaction Analysis (DiPIA) conferences and on LinkedIn,
Biacore™ SPR community.
Join our family, share your experiences, and learn from peers in the field of label-free

Biacore™ SPR systems

interaction analysis.

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More choices Biacore™ 1S+
for label-free Your discoveries — elevated.
Six flow-cell SPR system with exceptional sensitivity, full flexibility,

interaction analysis
and high capacity for reproducible interaction analysis of most
challenging targets or unstable binders with low response level.

From research through drug discovery and

development to QC. Choose the right system Biacore™ 1K+
Your development — advanced.
for your needs.
Six flow-cell SPR system with full flexibility for assay design
and high capacity for reproducible interaction analysis, with
straightforward transfer of methods between systems.

Biacore™ 8K+ Biacore™ 1K

Your discovery — accelerated. With maximized capacity. Your research — streamlined.
16 flow-cell SPR system that efficiently delivers binding data with Six flow-cell SPR system for robust, reproducible interaction
outstanding quality and maximized capacity, meeting your toughest analysis from day one with minimal effort. Affordable cost and
challenges in screening, characterization, process optimization, and reduced running expenses.
quality control.

Biacore™ 8K Biacore™ X100

Your discovery — accelerated. Your research — boosted.

Biacore™ SPR systems

16 flow-cell SPR system that efficiently delivers binding data with Two flow-cell SPR system for reliable insights into biological
outstanding quality and high capacity, meeting your toughest processes in multiuser environments.
challenges in screening, characterization, process optimization, and
quality control.

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cytiva.com/biacore
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doing business as Cytiva. Biacore and Biacore Intelligent Analysis are trademarks of
Global Life Sciences Solutions USA LLC or an affiliate doing business as Cytiva.
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For local office contact information, visit cytiva.com/contact

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