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    Experiment 2 Data

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    Hannah Kaye Cuesta
    The document describes several chemical tests performed on compounds like casein, egg albumin, tyrosine, cysteine, glucose, fructose, sucrose, lactose and maltose. It includes tables showing the observed color changes for each compound in Millon's test, ninhydrin test, sulfur test, Benedict's test, Barfoed's test and a hydrolysis test using Benedict's reagent.

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    Experiment 2 Data

    Uploaded by

    Hannah Kaye Cuesta
    13 views3 pages
    The document describes several chemical tests performed on compounds like casein, egg albumin, tyrosine, cysteine, glucose, fructose, sucrose, lactose and maltose. It includes tables showing the observed color changes for each compound in Millon's test, ninhydrin test, sulfur test, Benedict's test, Barfoed's test and a hydrolysis test using Benedict's reagent.

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    Experiment-2-Data

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    The document describes several chemical tests performed on compounds like casein, egg albumin, tyrosine, cysteine, glucose, fructose, sucrose, lactose and maltose. It includes tables showing the observed color changes for each compound in Millon's test, ninhydrin test, sulfur test, Benedict's test, Barfoed's test and a hydrolysis test using Benedict's reagent.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    13 views3 pages

    Experiment 2 Data

    Uploaded by

    Hannah Kaye Cuesta
    The document describes several chemical tests performed on compounds like casein, egg albumin, tyrosine, cysteine, glucose, fructose, sucrose, lactose and maltose. It includes tables showing the observed color changes for each compound in Millon's test, ninhydrin test, sulfur test, Benedict's test, Barfoed's test and a hydrolysis test using Benedict's reagent.

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    Table 2.

    Lactose composition in milk


    Mass of lactose in (g) 0.211 g
    Percent casein 4.22%
    Percent yield 27.7778%

    1. Millon’s Test
    Table 3. Determination of Tyrosine using Millon’s Test
    Compound Color Structure

    Casein Red color precipitate

    Egg albumin Red color precipitate

    Tyrosine Red color precipitate


    2. Ninhydrin Test
    Table 4. Determination of amino acids and proteins with ninhydrin test
    Compound Color Conclusions

    Casein Deep purple

    Egg albumin Purple top with white bottom

    Glycine Deep purple

    3. Sulfur Test
    Table 5. Determination of sulfur containing compounds using sulfur test.
    Compound Color Structure

    Cloudy white with dark brown


    Casein
    ppt

    Yellowish cloudy with dark


    Egg albumin
    brown layer and white ppt

    Cysteine Black precipitate


    1. Benedict’s Test
    Table 6. Identification of reducing sugars using benedict’s test
    Reducing Sugar
    Compound Color
    (Yes/No)

    Glucose Color changed from blue to red orange


    Fructose Color changed from blue to red orange

    No reaction, the same color was observed


    Sucrose
    (blue)

    Lactose Color changes from blue to red orange


    Maltose Color change from blue to red orange

    2. Barfoed’s Test
    Table 7. Identification of monosaccharides using Barfoed’s test
    Monosaccharide
    Compound Color
    (Yes/No)
    Glucose Color changed from blue to dark orange
    Fructose Color changed from blue to dark orange
    No reaction, the same color was observed
    Sucrose
    (blue)

    No reaction, the same color was observed


    Lactose
    (blue)

    No reaction, the same color was observed


    Maltose
    (blue)

    3. 3. Hydrolysis Test for Glucose


    Table 8. Hydrolysis reaction sugar using benedict’s test
    Compound Benedict’s reagent

    Glucose + HCl + NaOH Color changed from blue to red orange

    Fructose + HCl + NaOH Color changed from blue to red orange


    Sucrose + HCl + NaOH Color changed from blue to red orange

    Lactose + HCl + NaOH Color changed from blue to red orange

    Maltose + HCl + NaOH Color changed from blue to red orange

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