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Inventi Impact: Pharm Ana & Qual Assur Vol. 2012, Issue 2 94 2012ppaqa295, CCC: $10 © Inventi Journals (P) Ltd
[E- ISSN 0976–755X, P- ISSN 2229–4198] Published on Web 15/04/2012, www.inventi.in
RESEARCH ARTICLE
absorbance whereas the component of crossing point of CT and 239 nm, the zero Q-absorption Ratio Method
interest shows significant difference in crossing point of BD, was selected for the As shown in FigureǦ2, the overlain spectra of
absorbance with concentration [4, 5, 6, 7]. Based determination of CT. both drugs show a Reproducible IsoǦ
on this criterion, two wavelengths 237 nm absorptive point at 250.8 nm. Thus
and 241 nm were selected as λ1 and λ2 for Method Validation estimation of drugs by QǦabsorbance ratio
the estimation of CT. BD shows the same All the methods were validated as per ICH equation method was carried out at 250.8
absorbance at these wavelengths. Similarly, guidelines for parameters like solution nm (Isoabsorptive point) and 260.6nm
wavelengths 259 nm and 264 nm were stability, linearity, accuracy, precision, limit (λmaxof CT). The standard solutions of BD
selected as λ1 and λ2 for estimation of BD. of detection, limit of quantitation22. and CT were prepared to determine the
absorptivity values of the subject analyte at
First Derivative Spectroscopy Method RESULTS AND DISCUSSION the two selected wavelengths. The method
First derivative spectroscopy on the basis of In the present work, three methods, namely, showed good linearity in the range of
zeroǦcrossing measurements involves QǦabsorption ratio method, dual wavelength 6Ǧ34μg/mL for BD and 100-450μg/mL for
measurement of the absolute value of the method and first derivative spectroscopy CT.
total derivative spectrum at an abscissa value method were developed for the simultaneous
corresponding to them zeroǦcrossing spectroscopic estimation of CT and BD in Dual Wavelength Method
wavelength of the derivative spectrum of commercially available lotion dosage forms. In this method, standard solutions of CT and
another Methanol was used as the solvent since both BD were scanned in the entire range from
Component[4,5,6,7]. In this method, the drugs exhibit good solubility in it and no 200 nm to 400 nm. As shown in FigureǦ3, at
260.80nm was selected for the interference due to excipients of the lotion wavelengths of 237 nm and 241 nm, BD
determination of BD, which is the zero formulation were observed. shows same absorbance, and hence were
Inventi Impact: Pharm Ana & Qual Assur Vol. 2012, Issue 2 95 2012ppaqa295, CCC: $10 © Inventi Journals (P) Ltd
[E- ISSN 0976–755X, P- ISSN 2229–4198] Published on Web 15/04/2012, www.inventi.in
RESEARCH ARTICLE
Method Validation
The developed methods were validated for
parameters like solution stability,
Figure 1: Chemical structure of (A) Clotrimazole and (B) Beclomethasone dipropionate [8,9] linearity, precision, accuracy, LOD, LOQ.
the data for which are presented in the
Tables. The low value of R.S.D. value
indicates that all the methods are precise
and accurate.
Inventi Impact: Pharm Ana & Qual Assur Vol. 2012, Issue 2 96 2012ppaqa295, CCC: $10 © Inventi Journals (P) Ltd
[E- ISSN 0976–755X, P- ISSN 2229–4198] Published on Web 15/04/2012, www.inventi.in
RESEARCH ARTICLE
Inventi Impact: Pharm Ana & Qual Assur Vol. 2012, Issue 2 97 2012ppaqa295, CCC: $10 © Inventi Journals (P) Ltd
[E- ISSN 0976–755X, P- ISSN 2229–4198] Published on Web 15/04/2012, www.inventi.in