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Earthworm Dissection

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Guide to the Dissection of an Earthworm

1) To euthanize your earthworm, place it inside a small


glass jar with a cotton balls heavily soaked with 70%
alcohol. Cover the jar and wait for the worm to stop
moving.

2) Lay the worm on the dissecting tray with its dorsal


side facing up (dorsal side is darker in color). Stretch
the worm enough to steady the body across the tray
before securing the head end and posterior end with
pins.

3) Using your scalpel, start with a small (up to 1 cm)


dissection posterior/behind the clitellum, cutting
towards the tail (if your specimen does not have a
visible clitellum, start 1 inch below the head). Go
slowly and make it shallow enough to cut just the
skin (if you go too deep, you will puncture the
intestines, indicated by a blackish discharge).

4) Insert the tips of small dissecting scissors into the


opening and continue cutting the skin towards the
head end. Again, make sure to ONLY CUT THE SKIN.

5) Using your forceps, carefully pull apart one side of


the flap of skin. Carefully drag a pin along the inside
of the skin to sever the septum walls to make it
easier to spread the skin. Repeat the same on the
other flap of skin.

6) Once the septum walls are severed, pin the skin flaps
on the tray. Pin it diagonally.

7) Identify and study the internal organs


Skill-building Activities (with answer key)
• Read the instructions carefully and answer accordingly. Good Luck!

1) Identify and label the structures on the external anatomy of an earthworm.


2) Identify the structures on the internal anatomy of an earthworm and state their function.

Structure Function
1

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