Nothing Special   »   [go: up one dir, main page]
Scribd Logo
Upload

Welcome to Scribd!

  • 11 views

    ACTIITY 5 Biochem Answer WPS Office

    Uploaded by

    frishlovakiajalaidi
    The document discusses the purpose of various household reagents used in simple DNA extraction, including detergent, salt, meat tenderizer, and ice-cold ethanol. Detergent breaks down cell and nuclear membranes to release DNA. Salt neutralizes DNA molecules and detaches proteins. Meat tenderizer further breaks protein-DNA bonds and dissolves DNA. Ice-cold water and ethanol help precipitate DNA by slowing enzymes and allowing DNA to solidify. The document also lists the sugar, purine, and pyrimidine components of DNA and RNA along with their structural formulas.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd

    ACTIITY 5 Biochem Answer WPS Office

    Uploaded by

    frishlovakiajalaidi
    11 views2 pages
    The document discusses the purpose of various household reagents used in simple DNA extraction, including detergent, salt, meat tenderizer, and ice-cold ethanol. Detergent breaks down cell and nuclear membranes to release DNA. Salt neutralizes DNA molecules and detaches proteins. Meat tenderizer further breaks protein-DNA bonds and dissolves DNA. Ice-cold water and ethanol help precipitate DNA by slowing enzymes and allowing DNA to solidify. The document also lists the sugar, purine, and pyrimidine components of DNA and RNA along with their structural formulas.

    Original Description:

    Copyright

    © © All Rights Reserved

    Available Formats

    DOC, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document discusses the purpose of various household reagents used in simple DNA extraction, including detergent, salt, meat tenderizer, and ice-cold ethanol. Detergent breaks down cell and nuclear membranes to release DNA. Salt neutralizes DNA molecules and detaches proteins. Meat tenderizer further breaks protein-DNA bonds and dissolves DNA. Ice-cold water and ethanol help precipitate DNA by slowing enzymes and allowing DNA to solidify. The document also lists the sugar, purine, and pyrimidine components of DNA and RNA along with their structural formulas.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    11 views2 pages

    ACTIITY 5 Biochem Answer WPS Office

    Uploaded by

    frishlovakiajalaidi
    The document discusses the purpose of various household reagents used in simple DNA extraction, including detergent, salt, meat tenderizer, and ice-cold ethanol. Detergent breaks down cell and nuclear membranes to release DNA. Salt neutralizes DNA molecules and detaches proteins. Meat tenderizer further breaks protein-DNA bonds and dissolves DNA. Ice-cold water and ethanol help precipitate DNA by slowing enzymes and allowing DNA to solidify. The document also lists the sugar, purine, and pyrimidine components of DNA and RNA along with their structural formulas.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 2

    ACTIITY 5

    SIMPLE EXTRACTION OF DNA

    1. What is the purpose of using different household reagents in simple extraction of DNA?

    a. Detergent

    The purpose of detergent in DNA extraction is to break down the cell membranes and

    nuclear membranes, disrupting the lipid bilayers that surround the cellular and

    nuclear contents. Detergents are essential components of purpose of detergent in dna

    extraction protocols as they aid in releasing the DNA from the cellular and nuclear

    compartments, allowing subsequent purification and isolation of the genetic material.

    b. Salt

    The purpose of Salt in DNA extraction is to helps in neutralizing DNA molecules. It makes the DNA
    molecules less hydrophilic i.e less soluble in water. Salt also helps in detaching protein molecules from
    strands of DNA and makes the extraction process easier.

    c. Meat Tenderizer papaya extract

    The purpose of Meat tenderizer in DNA extraction is a Meat tenderizer is an enzyme that
    further breaks down the protein-DNA bonds and dissolves the DNA.

    d. Ice-cold ethanol

    Using ice-cold water and ice-cold alcohol will increase your yield of DNA. The cold water

    protects the DNA by slowing down enzymes that can break it apart. The cold alcohol helps

    the DNA precipitate (solidify and appear) more quickly.


    2. Enumerate the sugar component, purine and pyrimidine bases present in DNA and RNA and

    write their structural formula.

    DNA RNA

    1. Sugar A.Deoxyribose A. Ribose

    C5H10O4 C5H10O5

    2.purine A. Adenine B. Guanine A. Adenine B. Guanine

    C5H5N5 C5H5N5O C5H5N5 C5H5N5O

    3. Pyrimidine A. Cytosine B. Thymine A. Cytosine B. Uracil

    C4H5N3O C5H6N2O2 C4H5N3O C4H4N2O2

    3. Draw the structural formula of the following:

    You might also like