Mastercycler Pro: Register Your Instrument!
Mastercycler Pro: Register Your Instrument!
Mastercycler Pro: Register Your Instrument!
www.eppendorf.com/myeppendorf
Mastercycler pro
Operating manual
Manual de instrucciones
Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior
permission of Eppendorf AG.
Trademarks
Eppendorf®, the eppendorf logo, Mastercycler®, SteadySlope®, vapoprotect® and Eppendorf twin.tec® are
registered trademarks of Eppendorf AG.
Registered trademarks are not marked with ® or ™ in all cases in this manual.
Notice
The software of the Mastercycler pro contains open source software. License information is available as
PDF documents via the Eppendorf node. To export the license documents, connect a memory device, select
the Eppendorf node in the navigation tree and press the softkey.
Operating manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Manual de instrucciones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
6321 900.150-03/082013
Table of contents
Mastercycler pro 3
English (EN)
Table of contents
Operating
Mastercycler
on
see
p.
Tab.
Fig.
p.
English
Operating manual
pro
(EN)manual
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.2.1 Mastercycler pro and Mastercycler pro S . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.2.2 Mastercycler pro 384 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.3.1 Features of the Mastercycler pro variants. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.3.2 Networking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.3 Note on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.5 Warning signs on the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.1 Unpacking the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.2.1 Space requirements and bearing capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.2.2 Mains connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.3 Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.4 Shut down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1 Initial steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1.1 Using the vapo.protect heated lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1.2 Control panel keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.2 Initial start-up of the Mastercycler pro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.2.1 Defining the administrator PIN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.2.2 Other steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.3 User login and logout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.3.1 Logging in as a user . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.3.2 Changing the user . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.3.3 Logging out as a user . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.4 Overview of operation with the control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.4.1 Navigation tree overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.4.2 Using the navigation tree . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.4.3 Using external storage media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5.4.4 Selecting export file formats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Table of contents
4 Mastercycler pro
English (EN)
6 Programming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.1 Overview of the program editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.1.1 Opening the program editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.1.2 Structure of the program editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.2 Defining general settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.2.1 Opening the header. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.2.2 Editing header settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.3 Editing programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
6.3.1 Inserting a program step. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
6.3.2 Editing parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
6.3.3 Deleting individual program steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
6.3.4 Deleting all program steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
6.3.5 Exporting a program listing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
6.3.6 Saving a program and exiting the editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
7 PCR run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
7.1 Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
7.1.1 Selecting the sample tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
7.1.2 Loading the thermoblock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.1.3 Sample volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.2 Starting the program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
7.2.1 Status view . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
7.3 Interrupting or stopping the program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.3.1 Interrupting the program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.3.2 Continuing the program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.3.3 Stopping the program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.4 Using protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.5 Shutting down and switching off the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
8 System management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
8.1 Administrator functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
8.2 Managing user accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
8.2.1 Creating user accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
8.2.2 Editing user accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
8.2.3 Deleting user accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
8.2.4 Changing the PIN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
8.3 System settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
8.4 Cycler functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
8.4.1 Cycler system functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
8.4.2 Self test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
8.5 Managing and backing up data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
8.5.1 Backing up data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
8.5.2 Exporting and importing programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Table of contents
Mastercycler pro 5
English (EN)
9 Quick start. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
9.1 Login . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
9.2 Creating folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
9.3 Copying folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
9.4 Deleting folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
9.5 Editing programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
9.6 Stopping and starting programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
9.7 Switching off the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.1.1 Cleaning the housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.1.2 Cleaning the heated lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.2 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
10.3 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
11 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
11.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
11.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
13 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
13.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
13.2 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
13.3 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
13.4 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
13.5 Control panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
14 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
14.1 Mastercycler pro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
14.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
14.3 Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
14.4 Recommended auxiliary materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Table of contents
6 Mastercycler pro
English (EN)
Operating instructions
Mastercycler pro 7
English (EN)
1 Operating instructions
1.1 Using this manual
Read the operating manual before you operate the device for the first time.
Please view this manual as part of the product and keep it somewhere easily accessible.
If this manual is lost, please request another one. The current version of the operating manual can be
found on our website at www.eppendorf.com.
The sequences of actions in this operating manual are described for operation using the
control panel and the language setting English.
Representation Meaning
DANGER
Risk of electric shock with potential for severe injury or death as a consequence.
DANGER
Risk of explosion with potential for severe injury or death as a consequence.
DANGER
Bio hazard with potential for risk to health or death as a consequence.
DANGER
Risk of burns.
WARNING
Warning of potential injury or health risk.
CAUTION
Refers to risk of damage to property.
Refers to particularly useful information and tips.
Operating instructions
8 Mastercycler pro
English (EN)
Depiction Meaning
1. Actions in the specified order
2.
Actions without a specified order
• List
Text Display text or software text
Additional information
ESP
Electronic Sample Protection
PCL
Printer Command Language (standard for controlling printers)
PCR
Polymerase Chain Reaction
PDF
Portable Document Format
PIN
Personal Identification Number
PS
PostScript® (standard for controlling printers)
TSP
Thermal Sample Protection
USB
Universal Serial Bus
Product description
Mastercycler pro 9
English (EN)
2 Product description
2.1 Main illustration
Abb. 2-1: Mastercycler pro - front and rear view
1 2 34
7
8
11
9
10
1 2 3 4 5 6 7
1
2
3
4
Only devices meeting the IEC 950/EN 60950 (UL 1950) standards may be connected to the interfaces of the
Mastercycler pro and the control panel.
Product description
Mastercycler pro 11
English (EN)
The contents of the delivery package depend on the variant of your Mastercycler pro.
2.3 Features
Product variants
The Mastercycler pro is available in three variants.
• Mastercycler pro
• Mastercycler pro S
• Mastercycler pro 384
SteadySlope
SteadySlope Technology ensures that the heating and cooling ramp rates for thermal modules are identical
in both gradient and normal operation. That means you can expect identical temperature control
characteristics in both optimization and routine experiments, as well as a reliable transfer of the
optimization results to the routine application.
vapo.protect
All variants of the Mastercycler pro have avapo.protect heated lid. A flexible pressure element ensures that
force is applied evenly to the surface of the tubes or plate. This reduces evaporation from the reaction
sample effectively. The vapo.protect heated lid performs automatic tube height adjustment for all sample
tubes and PCR plates.
TSP
The heated lid of the Mastercycler pro series is equipped with TSP technology (Thermal Sample
Protection). The thermoblock is continuously kept at 20°C during the lid heating phase. This means TSP
reduces the thermal load on your samples and minimizes the probability of non-specific product formation
during the PCR.
Self-test function
You can use the integrated self test function of the Mastercycler pro in combination with the control panel
with USB ports to check whether the thermoblock is functioning properly without the need for any further
tools. A separately available USB key is required for the self test function (see Accessories on p. 85).
Control
The Mastercycler pro is controlled via the control panel. It can also be controlled via the PC software
CycleManager pro.
Product description
Mastercycler pro 13
English (EN)
Variant Features
Mastercycler pro • Freely programmable temperature gradient over 12 rows.
• A gradient range of up to 20°C.
• Robust, specially coated aluminum block.
• Holds up to 96 PCR tubes (0.2 ml) or a 96-well PCR plate.
Mastercycler pro S • Highest temperature control speed due to silver block: up to 6°C/s.
• Standard PCR possible in under 30 min.
• Pulse function for device-driven hot start for avoiding non-specific PCR product
formation.
• Freely programmable temperature gradient over 12 rows.
• Extended gradient range of up to 24°C.
• Holds up to 96 PCR tubes (0.2 ml) or a 96-well PCR plate.
Mastercycler pro 384 • Freely programmable temperature gradient over 24 rows.
• A gradient range of up to 20°C.
• Holds a 384-well PCR plate.
2.3.2 Networking
Mini-satellite system
Up to five devices from the Mastercycler pro or Mastercycler ep series can be interconnected and operated
via a central control panel. The procedure for setting up this type of configuration is described separately
(see Operating multiple Mastercycler pro via a control panel ("mini-satellite" system) on p. 23).
PC control
You can use the PC software CycleManager pro to control up to 30 devices from the Mastercycler pro or
Mastercycler ep series in a network. This software is available separately for purchase (see Accessories on
p. 85).
Product description
14 Mastercycler pro
English (EN)
Safety
Mastercycler pro 15
English (EN)
3 Safety
3.1 Intended use
The Mastercycler pro is intended to be used in research and routine laboratories in the field of biomedical
sciences.
The Mastercycler pro is used to control the temperature of aqueous solutions, suspensions, and emulsions
in closed micro test tubes for enzymatic reactions, typically for polymerase chain reaction (PCR).
This device must only be used by skilled personnel with the appropriate training.
Before using the device, read the operating manual carefully and familiarize yourself with the device's
mode of operation.
In the following cases the protection provided in the device may be impaired. The liability for the function
of the device passes to the operator if:
Read the operating manual first and follow the general safety instructions below before using the
Mastercycler pro.
WARNING! Risk to health and bio hazard when operated with lid open.
The tube lids may pop open at high block temperatures.
Close the lid of the device when heating samples.
This section explains the warning signs used on the Mastercycler pro.
1
2
1 WARNING
Risk of burns from the hot thermoblock (triangle with tip
facing down) or the hot inner side of the heated lid
(triangle with tip facing up).
Gradient operation can result in the temperature being
hotter on the right side than on the left side
2 WARNING
Risk of burns from hot surfaces.
Safety
20 Mastercycler pro
English (EN)
Installation
Mastercycler pro 21
English (EN)
4 Installation
4.1 Unpacking the device
1. Release the four plastic locking clips at the long sides of the carton by pulling out the flaps.
2. Lift the carton lid and take the foam upper part off the Mastercycler pro.
3. Take the carton insert out of the carton lid (contains the mains cable).
4. Take the Mastercycler pro out of the plastic bag.
5. With both hands, hold the Mastercycler pro by the lower half of the housing and lift it out of the
package.
6. Remove all the packing material.
7. Visually check the device to ensure that no damage has occurred during shipment.
8. Check the completeness of the delivery (see Delivery package on p. 11).
Never use a device if it shows any signs of damage. In this case, contact your distributor or the
appropriate Eppendorf office.
• Retain the packing material for shipping or storing the Mastercycler pro at a later date.
• Ship the Mastercycler pro only in the original packing material.
Installation
22 Mastercycler pro
English (EN)
Information regarding the dimensions and weight of the device components can be found separately(see
Weight/dimensions on p. 83).
When selecting a location for the Mastercycler pro ensure that the following criteria are met:
• The surface must have sufficient bearing capacity and ensure the required stability.
• The feet at the front of the unit must be secured to prevent them from slipping.
• The device must be positioned at a height which provides for convenient and safe operation.
• Additional space is required for free air circulation at the back of the device (10 cm) and for the control
panel at the front (14 cm).
CAUTION! Connected devices may be shut down due to a power supply overload.
The total current consumption of the devices can exceed the maximum permitted supply
rating when multiple power sockets are used. In this case, the mains fuse interrupts the
electric circuit. The connected devices are shut down.
Do not use multiple sockets when connecting several Mastercycler pro.
Information on the power consumption of the Mastercycler pro is provided separately (see Power supply on
p. 83).
• You need an earthed wall socket for the power supply of the Mastercycler pro. Use the supplied power
cable to connect the Mastercycler pro.
• The control panel is supplied via the Mastercycler pro and does not require an additional power supply.
Installation
Mastercycler pro 23
English (EN)
This section describes how to start up the Mastercycler pro with the control panel and how to operate
several devices belonging to the Mastercycler pro and Mastercycler ep series with each other in a network.
The connections and switches are located at the back of the device (see Main illustration on
p. 9).
• Follow the steps below to define the settings and connect the devices before switching on
the Mastercycler pro.
• Never disconnect the control panel from a Mastercycler pro that is switched on.
1. Connect the cable on the control panel to the Control Panel connector of the
Mastercycler pro.
3. Use the address dials to set the address of the Mastercycler pro to 01. To do this,
turn the left dial into position 0 and the right dial to 1, as shown in the illustration.
4. Connect the power cable to the Mastercycler pro and to the mains supply.
4.3.2 Operating multiple Mastercycler pro via a control panel ("mini-satellite" system)
CAUTION! Connected devices may be shut down due to a power supply overload.
The total current consumption of the devices can exceed the maximum permitted supply
rating when multiple power sockets are used. In this case, the mains fuse interrupts the
electric circuit. The connected devices are shut down.
Do not use multiple sockets when connecting several Mastercycler pro.
A control panel can be used to control up to five devices belonging to the Mastercycler pro or Mastercycler
ep series (also in combination). Each additional device connected requires its own CAN bus cable. CAN bus
cables are available separately for purchase (see Accessories on p. 85).
Installation
24 Mastercycler pro
English (EN)
1. Connect the cable on the control panel to the "Control Panel" connector of the first
device.
CAN_out 2. Connect the CAN bus cable to the "CAN_out" connector of the first device.
CAN_in 3. Connect the free end of the CAN bus cable to the "CAN_in" connector of the
second device.
Term 5. Set the Term switch of the first and last device to "ON" (lower position).
Term 6. Set the Term switch of all other devices to "OFF" (upper position).
Note: If the network consists of exactly two devices, the Term switch of both
devices must be set to "ON".
7. For the first device connected to the control panel, set the address to 01.
CAN_out CAN_in Term Address CAN_out CAN_in Term Address CAN_out CAN_in Term Address Control Panel
Fig. 4-1: Address and Term settings and wiring connections for a network consisting of three devices
You can extend an existing system of one or more Mastercycler ep devices with a corresponding control
panel using devices of the Mastercycler pro series.
When operating the Mastercycler pro with a Mastercycler ep control panel (without USB
connections), the following restrictions apply:
• The self test function is not available.
• The PDF file format is not available for exporting programs, protocols and log files.
• No USB devices can be connected. Information for connecting a PS/2 mouse can be found
in the Mastercycler ep operating manual.
Prerequisites
Before a Mastercycler pro can be connected to an existing Mastercycler ep system, the software of the
control panel and of the connected Mastercycler ep has to be updated. For more information, contact the
Eppendorf branch for your country (see back cover flap).
1. Follow the instructions in the Mastercycler ep operating manual to update the control panel software.
2. After restarting the control panel, synchronize all connected Mastercycler ep with the control panel.
3. Shut down the control panel (see p. 54) and switch off all Mastercycler ep devices.
4. Set the address of the new Mastercycler pro to the next free address number.
Example:
If you already operate two Mastercycler ep devices, set the address of the new
Mastercycler pro to 03.
5. Connect the cables and define the Term settings.
6. Switch all devices on and register them at the control panel.
The Mastercycler pro is displayed as an additional cycler node on the control panel.
The control panel of the Mastercycler pro has two USB ports (see Fig. 2-3 on p. 10) for connecting a
mouse, printer, storage medium or the self test USB key.
If you would like to use more than two USB devices at the control panel at the same time, you can make
use of a USB hub.
Installation
26 Mastercycler pro
English (EN)
The Mastercycler pro can be connected to a parallel interface printer in order to print protocols and
programs in text format.
Connect the printer via a parallel data cable to the parallel interface (see Fig. 2-2 on p. 10) of the
Mastercycler pro.
A printer with USB interface can be connected to the control panel to print protocols, programs and self
test certificates.
Prerequisites
The USB printer must be compatible with the PostScript® or PCL printer standards. Further information
can be found in the operating manual for your printer.
1. Us a USB cable to connect the printer to one of the two USB ports (see Fig. 2-3 on p. 10) of the control
panel.
You can connect a USB mouse (not included in the delivery package) to the control panel.
Connect the plug of the mouse to one of the USB ports on the right-hand side of the control panel (see
Fig. 2-3 on p. 10).
The mouse pointer appears in the display.
Proceed as follows if you do not want to use the Mastercycler pro for longer than a week.
Do not leave the lid of the Mastercycler pro in an open position in order to avoid the block from becoming
contaminated.
Operation
Mastercycler pro 27
English (EN)
5 Operation
5.1 Initial steps
Before the Mastercycler pro is started up for the first time, ensure the following:
• The device is correctly connected.
• The device is free of damage.
• Make sure that air can circulate freely around the ventilation slots on the rear of the device and around
the fan behind the feet.
Thevapo.protectheated lid features a heated flexible pressure unit. When the lid closes, the pressure unit
comes to rest lightly on the surface of the sample tubes. A liquid cushion in the pressure unit is then used to
create a pressure to ensure that the tubes are pressed uniformly into the thermoblock thereby protecting
the tube seals against evaporation. The heating process prevents condensation from forming in the upper
area of the tube when the reaction liquid is heated.
Prerequisites
The thermoblock of the Mastercycler pro is loaded with sample tubes or a PCR plate (see Loading the
thermoblock on p. 50).
1. Pull vapo.protect heated lid forwards at the lid handle as far as possible so that the lid handle can be
rotated.
2. Flip the handle over the heated lid until it clicks into place.
You can now start a program run or manually temperature-control the lid and the samples.
WARNING! Risk to health and bio hazard when operated with lid open.
The tube lids may pop open at high block temperatures.
Close the lid of the device when heating samples.
WARNING! Risk of burns from the hot thermoblock and heated lid.
The thermoblock and the heated lid very quickly reach temperatures above 95 °C.
Keep heated lid closed until the temperature of the thermoblock is below 30 °C.
Flip the lid handle up over the heated lid and push the heated lid by the handle to the rear position until
it stops.
Operation
Mastercycler pro 29
English (EN)
The vapo.protect heated lid is equipped with a status lamp 2(see Main illustration on p. 9) which allows the
current operating status of the Mastercycler pro to be visible from far off.
The vapo.protect heated lid is equipped with a two-digit address display so that the cycler can be identified
easily (see Main illustration on p. 9).
The displayed address number matches the cycler numbering in the operating software. The address
setting is described separately.
Operation
30 Mastercycler pro
English (EN)
1 2 3
abc def
Stop
4 5 6
ghi jkl mno
7 8 9
pqrs tuv wxyz
Start
0 . Delete
Enter
Next
Function keys
The function of the key changes with the software dialog and appears in the display
immediately above the key.
• Step-by-step navigation.
The current position of the cursor is marked.
• Move the cursor within an alphanumerical input field.
4 5 6 In the case of numerical input fields (e.g. temperature and time entries) the keys
ghi jkl mno
only represent numbers. For decimal numbers with numbers after the decimal
7 8 9
pqrs tuv wxyz point, use the period as a decimal point.
0 . Delete
Alphanumerical input fields have keys with multiple functions.
To enter text, keep pressing the key with the relevant letter until the desired letter
is displayed.
Example: To enter pcr 3 in a text field, press the following keys in quick succession:
1. for p: press 7 once
2. for c: press 2 three times
3. for r: press 7 three times
4. for the blank space: press 0 (zero) twice
5. for 3: press 3 four times
Operation
Mastercycler pro 31
English (EN)
During the initial startup of the control panel you can define an administrator PIN in order to protect the
system against access by unauthorized persons. These data are saved in the control panel and are not lost
when the Mastercycler pro is switched off.
If you do not want to use the user management option, you do not have to define an
administrator PIN. In this case, leave the input fields of the Input Admin PIN window empty
and deactivate the PIN function. Further information on user management is provided
separately (see p. 58).
Operation
32 Mastercycler pro
English (EN)
To define the administrator PIN, perform the following steps in the sequence described.
1 2 3 2. Enter the desired administrator PIN using the numerical keys on the control panel.
abc def
The PIN can be between 4 and 8 digits long.
4 5 6
ghi jkl mno
7 8 9
pqrs tuv wxyz
0 . Delete
4. If your two PIN entries do not match each other, an error message is displayed. In
Delete
this case, delete the incorrectly entered PIN and repeat the PIN entries.
5. Confirm by pressing the OK function key.
After successful confirmation of the administrator PIN, the navigation tree appears in the control panel
display. You are now logged in as the administrator.
• If the PIN function is activated, you must define an administrator PIN. Otherwise this input
prompt will appear each time the device is switched on.
• You can also change the administrator PIN subsequently (see Changing the PIN on p. 56).
The date and time of the control panel are displayed in the upper right-hand corner of the display. As the
administrator you can change this internal clock at any time (see p. 58).
To be able to use the Mastercycler pro, you must set up at least one user account. Detailed information
about setting up user accounts and about user administration can be found separately(see Managing user
accounts on p. 55).
Operation
Mastercycler pro 33
English (EN)
The functions described here are only available if the PIN function is activated.
Before you can log in under your user name, your administrator must have set up a user account for you.
You are now logged in under your user name and can work with the Mastercycler pro.
You can log yourself out from the control panel to protect your programs against unauthorized changes.
1
2
3
4
5
6
Abb. 5-1: Navigation tree of the Mastercycler pro. The MMC and USB nodes are only visible if a storage medium has been inserted.
Fig. 5-1: Navigation tree of the Mastercycler pro. The MMC and USB nodes are only visible if a storage
medium has been inserted.
Mark the node with the arrow keys and press the Enter key or the arrow key .
This opens the node and the plus symbol in front of the node changes to a minus
symbol.
Or click on the plus symbol in front of the node with the mouse.
Use the mouse to click on the minus symbol in front of the node.
Or mark the node with the arrow keys and press the Enter key or the arrow key .
This closes the node and the minus symbol in front of the node changes to a plus
symbol.
You can save programs, log files and protocols on an MMC memory card or a USB storage medium and
then, for example, archive or print them out on a PC. External storage media are also needed for data
backups and software updates.
The status of the USB ports is indicated by the following symbols in the upper right-hand
corner of the control panel:
• USB ports deactivated. The USB ports cannot be used if the control panel is connected
to a Mastercycler ep.
Connect the control panel to a Mastercycler pro to activate the USB ports.
• USB error. A faulty or incompatible USB device has been connected.
Disconnect all USB devices and switch the control panel off. After restarting, all USB ports
will be reactivated.
The control panel creates a folder called \eppendorf on the USB storage medium where all
files will be saved.
Operation
38 Mastercycler pro
English (EN)
Removing the USB storage medium Removing the MMC memory card
Caution! The symbol is displayed in the upper
right corner while the USB storage medium is being Caution! The symbol is displayed in the upper
accessed. Wait until it has disappeared before right corner while the MMC is being accessed. Wait
removing the USB storage medium. until it has disappeared before removing the MMC.
Remove the USB storage medium. 1. Briefly press on the projecting end of the inserted
MMC.
2. Pull the MMC out of the slot.
The USB node disappears from the navigation tree. The MMC node disappears from the navigation tree.
When exporting programs, protocols and log files to external storage media, you can choose between
text-only format (.TXT) and PDF.
Select memory device Select USB or MMC as the storage medium for the file you would like to
export, if more than one storage medium is connected to the system.
Select file format Selecting the file format:
• PDF: Saves the file as PDF.
Use this setting if you only want to print or view the documents on your
PC. You need to have a PDF reader software installed (e.g. Adobe®
Reader®).
• Text-only (.TXT): Saves the file in text-only format (.TXT). Use this
setting if you want to process the document content electronically (e.g.
embed it in a report).
If you are logged in as a normal user (not as the administrator), you are only authorized to
create, edit and delete programs and folders below your own user node. More information on
user rights can be found in a separate section(see Administrator functions on p. 55).
Proceed as follows:
4. To enter a comment for the new folder, select the Next key in the Comment field.
5. Enter a comment text using the numerical keys and confirm by pressingOK.
The folder is created and appears in the navigation tree under your user node.
You can change the name and the comment of the folder at any time using theProp. function
key.
Proceed as follows:
1. In the navigation tree, open your own user node and possibly the folder in which you want to create the
new program.
2. Press the New function key.
Operation
40 Mastercycler pro
English (EN)
The New Cycler Program window is displayed. You can enter a name and a comment for the new
program using the numerical keys here.
You can change the name of the program or the comment at any time using the Prop. function
key.
To protect a program against inadvertent changes, you can use the Prop. function key to mark
it as write-protected (read only). This means the program can then be displayed in the
program editor(see p. 41), but not changed. This is recommended when working without a
PIN.
You can copy folders and programs to your own user nodes as follows. When copying a folder, the
programs it contains are also automatically copied.
1. In the user node belonging to any user, mark the folder or the program you want to copy.
2. Press the Copy function key.
A message appears informing you that the folder or the program was copied to the clipboard.
3. Confirm the note with OK.
4. Mark your own user node. If you want to copy a single program, you can also select a folder below your
user node to which you want to insert the program.
5. Press the Paste function key.
If a program or folder already exists with the same name, you will be prompted to enter a new name.
The folder or program is inserted under your user node. You can now start or edit this program.
6 Programming
6.1 Overview of the program editor
The graphic program editor can be used to view and change existing programs.
1
6
2 7
8
3
4
9
5
10
Each Mastercycler pro program features a program header in which you can define the block temperature
control mode and heated lid behavior. These settings are valid for the entire program.
1. Open the program editor (see Opening the program editor on p. 41).
1 StepCyc Cycle program step with 1 to 3 temperature steps with an adjustable number of
to3 StepCyc repetitions (1 to 99).
With cycle program steps you can increase or reduce the block temperatures and hold
times by a preset value after each cycle (see Editing parameters on p. 45).
n StepCyc Cycle program step with an adjustable number of temperature steps. After selecting
this program step and confirming by pressingEnter a dialog window appears for the
following entries.
• Steps per Cycle: Number of temperature steps per cycle (1 to 20)
• Number of Cycles: Number of cycles (1 to 99). If you have inserted a cycle with
more than 6 steps, use the arrow keys and to display the hidden steps.
Temp Individual temperature step with adjustable block temperature and hold time.
Hold Temperature step with adjustable block temperature and indefinite hold time.
The set temperature is maintained until the user presses the Enter key. A
corresponding prompt is displayed in the status view and also indicated by the status
light flashing orange (see Status lamp on p. 29).
Sound This program step emits an acoustic signal.
Pause Like Hold. The block temperature remains set to the value of the previous temperature
step.
You can now insert additional program steps or edit the parameters of the program steps.
Programming
Mastercycler pro 45
English (EN)
3. Use the numerical keys to enter a new value between 4.0 °C and 99.0 °C (in increments of 0.1 °C).
4. Confirm by pressing the Enter key.
Changing the hold time
5. Mark the hold time.
6. Use the numerical keys to enter a new value between 000:00 to 999:59 (mmm:ss, in increments of 1 s).
7. Confirm by pressing the Enter key.
Changing the number of cycles
8. Mark the value for the repetitions.
You can export a program to an external storage medium as a file for documentation purposes.
7 PCR run
7.1 Preparation
7.1.1 Selecting the sample tubes
The Mastercycler pro can be used with a great variety of sample tube and plate types. Consider the
following when selecting sample tubes, plates and lids:
• Only use sample tubes, plates and lids which have a heat resistance of up to approx. 120 °C.
• The sample tubes and plates must fit into the thermoblock bores free from play to achieve a good heat
transfer.
PCR run
50 Mastercycler pro
English (EN)
The Mastercycler pro and Mastercycler pro S can be loaded with 0.2 mL single tubes or strips. You can also
use a 96-well PCR plate. The following PCR plate types are compatible with the Mastercycler pro:
PCR plate with surrounding PCR plate with a surrounding PCR plate without any surrounding
half-edge. edge. edge.
The Mastercycler pro 384 can be loaded with a 384-well PCR plate.
To seal the PCR plates, you can use adhesive and heat sealing films, lid strips or reusable silicone mats.
A selection of the PCR micro test tubes and lids can be found separately (see Consumables on p. 86).
The liquid level in the micro test tube should not be above the upper edge of the block.
You can use the following standard values for the maximum sample volumes:
• Mastercycler pro and Mastercycler pro S: Maximum 100 μL per single reaction.
• Mastercycler pro 384: Maximum 25 μL per single reaction.
The sample volumes that can actually be used may vary depending on the type of micro test tube used.
PCR run
Mastercycler pro 51
English (EN)
Start the PCR programs from the editor or the navigation tree.
If the PIN function is activated, you can only start programs which are stored below your user node (see
p. 55).
Prerequisites
You are logged in as user and the program to be started is saved.
1. Mark the program in the navigation tree with the arrow keys or open it in the editor.
2. Insert the sample tubes (see Loading the thermoblock on p. 50).
3. Close the heated lid (see Closing the heated lid on p. 27).
(For devices from the Mastercycler ep series with motorized lid, this will be closed automatically when
the program is started.)
4. Press the Start key.
If several cyclers are connected, the Cycler Selection dialog appears.
1
6
7
8
2
3
9
4
5
If you have activated the TSP/ESP function in the program header (see p. 42), the heated lid is
heated or cooled to the desired temperature before the program run starts. This process is
indicated in the status view by the message TSP Heated Lid.
You can exit the status view at any time via the Exit function key to return to the navigation tree and, for
example, edit another program or start a program on a different cycler. The program that was already
started is not interrupted as a result.
Proceed as follows:
The cycler is indicated in the navigation tree with a green symbol while it executes a
program.
PCR run
Mastercycler pro 53
English (EN)
You can temporarily interrupt the program run in the status view, for instance, to add reagent, or
completely stop the program.
Observe the safety precautions for opening the heated lid (see Opening the heated lid on p. 28).
1. If the heated lid is opened, draw the heated lid forwards over the samples and close the lid.
2. Press the Resume function key.
The status view displays running and the program run is continued.
The Mastercycler pro creates a protocol for each program run where all executed program steps and any
interruptions and errors are recorded.
After running a PCR program, you can view the associated protocol on the control panel, save it on an MMC
or a USB memory or send it to a printer.
The protocol is overwritten the next time a program is started on the Mastercycler pro.
Prerequisites
The cycler is idling.
3. Use the function keys << and >> to go one page back or forward.
Use the arrow keys on the control panel to move the visible section.
Further functions for saving and printing the protocol are available in the protocol screen.
Shut down the control panel before you shut down the Mastercycler pro:
8 System management
8.1 Administrator functions
The software of the Mastercycler pro differentiates between standard users and the administrator.
ThePIN setting can be used to define whether all users have to log in with their user names and PINs before
using Mastercycler pro or whether all users should receive administrator rights (without entering their user
names and PINs).
1. In the navigation tree, mark the Eppendorf node at the very top.
2. Press the NewUser function key.
TheNew User window is displayed.
System management
56 Mastercycler pro
English (EN)
3. Enter the name and PIN for the new user. Repeat the PIN entry in the Confirmation field.
4. Confirm by pressing the OK function key.
The new user account is set up. In the navigation tree, a new user node is displayed with this user name.
The user can now log in with his or her PIN at any time.
You can subsequently change the user name, the PIN and the comment for an existing user account
depending on your user rights (see p. 55):
1. In the navigation tree, mark the user node and press the Prop. function key.
The User Properties window is displayed.
2. Change the settings for the user account and confirm with the OK function key.
When a user account is deleted, the user node selected as well as all its folders and programs
are deleted from the navigation tree. Deletion of a user account cannot be undone.
1. In the navigation tree, mark the user node and press the Delete key.
A prompt appears asking you if you are sure you want to delete the user.
2. Confirm by pressing the OK function key.
The user account is deleted.
If you work with the PIN function, only the administrator is authorized to make system settings and manage
user accounts.
System management
Mastercycler pro 57
English (EN)
If you are logged on as an administrator, you can change the administrator PIN at any time.
You can change the PIN for logging into your user account at any time. To do this, proceed as follows.
1. In the navigation tree, mark your own user node and press the Prop. function key.
Show free Mark the node System memory and press the function key Show.
system memory The system memory is shown as a red-green marking at the lower end of the display
(red for the used memory, green for free memory).
Press the Show function key again to hide the display.
Exporting the Mark the Logfile node and press the Export or the Export 20 function key.
log file The log file or the last 20 entries of the log file are written to an MMC or USB storage
medium.
The log file can be displayed using the function key Show.
Changing date 1. Mark the node Date + Time and press the function key Set.
and time 2. Set date and time and confirm using OK.
Change country The country settings are indicated with the international area code (e.g. 001 for USA).
settings The language (German or English) as well as the date and time format (12 or 24 hour
format) are defined in the country settings.
1. Mark the node Country Settings and press the function key Set.
2. Select the required settings and confirm using OK.
The Mastercycler pro is immediately shut down. The modified settings apply after the
next start.
Activating or Mark the node Beeper and activate or deactivate it.
deactivating the If the red checkmark is set, the acoustic signal for error messages and key presses is
acoustic signal activated.
Activating or Use the PIN node to define whether or not a PIN entry is required for working.
deactivating • Mark the node PIN and activate or deactivate it.
PINs
The control panel is immediately shut down. The modified settings apply after the
next start.
System management
Mastercycler pro 59
English (EN)
Performing a Use the update node to perform a software synchronization between the cycler and the
software control panel. The software synchronization may be necessary if you receive error
synchronization messages after updating the software of the control panel.
1. Highlight Update node.
2. Press Cycler function key.
3. Select the Cycler.
4. Press Start.
Exporting Use the UserLog node to export or display all the actions that were performed on the
user-related data control panel as a log file.
You can save login and logout information, all changes and interventions in a running
program related to specific users.
Mark UserLog and press the desired function key:
Show: Display user log. You can print or export the user log in the display.
Exp+Clear: Transfer the user log to the MMC or USB storage medium and delete
the log from the control panel afterwards.
Export: Copy the user log to the MMC or USB storage medium.
System management
60 Mastercycler pro
English (EN)
In the Cycler node, you can view and adjust the status of the thermoblock and the heated lid. You can also
retrieve the protocol of the last PCR program used.
The System node contains the cycler system level with the following function keys.
Block Display block type, serial number as well as the date when the last validation and
calibration took place.
Restart Entry for maximum duration of interruption of voltage supply (mm:ss) after which a
running program should be automatically restarted. The program is not restarted after
a longer interruption.
Sensors Current sensor temperatures for the lid and block.
Validation Check block temperature control with the temperature validation system.
Calibration Calibrate and adjust block temperature control with the temperature validation
system. Only displayed if the administrator is logged in.
Using the functions Validation and Calibration requires the Eppendorf temperature validation
system (see Accessories on p. 85). For information on how to use this system, refer to the
corresponding operating manual.
System management
Mastercycler pro 61
English (EN)
The self test function of the Mastercycler pro allows you to perform a quick check regarding the following
attributes of the thermoblock without requiring an external measurement system:
A separately available USB key is required for performing the self test (see Accessories on p. 85). Please
refer to the documentation of the self test USB key for a detailed description of the self test function.
The function keys in the above view show the options available to the administrator for transferring data.
Standard users only have the option of performing backups here.
Insert an MMC memory card or a USB storage medium into the right-hand side of the control panel.
The node MMC or USB will be displayed in the navigation tree.
Select the MMC or USB node.
System management
62 Mastercycler pro
English (EN)
Backup Back up user nodes, folders and programs onto an external storage medium.
• MMC: The MMC inserted will automatically be completely deleted at the start of
the backup process.
• USB: Any files and folders in the directory \eppendorf will be deleted automatically
at the start of the backup process.
The backed-up data can be copied into the control panel using the Restore function.
Restore Administrator only: copy the user nodes, folders and programs saved on the external
storage medium during a backup to the control panel.
This process deletes any user nodes, folders and programs which already exist in the
control panel. After the restore process, only the data copied from the external storage
medium will be available.
Export
1. Insert an MMC or a USB storage medium into the slot on the right-hand side of the
control panel.
2. Open the node MMC or USB and mark the folder or program you would like to
import.
3. Press the function key Copy.
4. Mark the user node or folder in the destination and press the function key Paste.
System management
Mastercycler pro 63
English (EN)
The auto update function automatically initiates a software update on the control panel.
Prerequisites
• You are logged on as an administrator or the PIN function is switched off.
• All connected cyclers are idling.
• The navigation tree is displayed.
1. Log on as administrator or deactivate the PIN function.
2. Insert MMC memory card or USB storage medium with the new software version.
3. Confirm query with Yes.
The software version currently installed and the software version on the storage medium are displayed.
4. Press the Update function key to start the update. You can cancel the operation using the Restart
function key.
The update may take several minutes. Afterwards, the control panel will automatically restart.
If the automatic update was canceled or did not start, you can also perform a manual update.
Prerequisites
• You are logged on as an administrator or the PIN function is switched off.
• An MMC memory card or a USB storage medium with a newer software version is inserted into the
control panel.
1. Highlight MMC node or USB.
2. Press Firmware function key.
Always complete the software update for the control panel first, and then the update for all
connected Cyclers (software synchronization).
System management
64 Mastercycler pro
English (EN)
3. Select Software Update Control Panel entry and press the Start function key.
The software version currently installed and the software version on the storage medium are displayed.
4. Press the Update function key to start the update. You can cancel the operation using the Restart
function key.
The update may take several minutes. Afterwards, the control panel will automatically restart.
PCR programs established on the Mastercycler (5333) or the Mastercycler gradient (5331) can easily be
transferred to the Mastercycler pro without first having to optimize the temperatures and hold times.
Example
1 T = 94.0° 0:02:00
2 T = 94.0° 0:00:15
3 T = 60.0° 0:00:15
+0.0° +0:00
R = 3.0°/s +0:0°/s
G =10.0°
4 T = 72.0° 0:00:30
5 GOTO 2 REP 29
6 T = 72.0° 0:02:00
7 HOLD 4.0° ENTER
System management
Mastercycler pro 65
English (EN)
Setting from the Mastercycler 533x Corresponding setting in the program header
program
LID 105 °C Lidtemp 105 °C
Wait TSP/ESP heated lid
9 Quick start
9.1 Login
1. Open list.
Enter
2. Select user.
3. Confirm selection.
Enter
1 2
abc
3
def
5. Enter and confirm PIN.
4 5 6
ghi jkl mno
7 8 9
pqrs tuv wxyz
0 . Delete
Enter
Quick start
68 Mastercycler pro
English (EN)
1 2
abc
3
def
3. Enter and confirm names and
4 5 6 comments.
ghi jkl mno
7 8 9
pqrs tuv wxyz
0 . Delete
Enter
3. Confirm selection.
Enter
7. Confirm input.
Enter
Quick start
70 Mastercycler pro
English (EN)
4 5 6
ghi jkl mno
7 8 9
pqrs tuv wxyz
0 . Delete
1 2 3
abc def
4 5 6
ghi jkl mno
7 8 9
pqrs tuv wxyz
0 . Delete
6. Confirm input.
Enter
10 Maintenance
10.1 Cleaning
We recommend that you regularly clean the surfaces of the Mastercycler pro.
Clean the surfaces of the Mastercycler pro with a damp, lint-free cloth. Use a neutral lab cleaner if
necessary.
Instructions on disinfecting and decontaminating the device are described separately(see Disinfection/
decontamination on p. 76).
2. To open the lock, press the left and right latches underneath the front edge of the heated lid upwards
(1).
3. Fold up the heated lid (2) until the stand (4) is locked.
Maintenance
Mastercycler pro 75
English (EN)
3
4
4. Remove any contamination in the area of the block and on the inner side of the lid with a damp, lint-free
cloth. Use a neutral lab cleaner if necessary. Remains of markings from tube lids on the heating unit can
be removed with ethanol or isopropanol.
Keep the block bores free of dust and lint.
Even small amounts of deposits can affect the heat transfer between the block and sample
tubes.
1. While holding the front edge of the lid with your right hand, pull the stand 1forwards with your left
hand.
2. Lower the heated lid slowly until it locks into the horizontal position.
Maintenance
76 Mastercycler pro
English (EN)
10.2 Disinfection/decontamination
DANGER! Electric shock as a result of penetration of liquid.
Switch off the device and disconnect it from the power supply before starting cleaning or
disinfecting.
Do not allow any liquids to enter the inside of the housing.
Do not perform spay disinfection.
Only reconnect the device to the power supply once it is completely dry.
Required equipment
• Alcohol (ethanol, isopropanol) or disinfectants containing alcohol
• Mild, soap-based household cleaning agent
Proceed as follows:
1. Select a disinfection method that complies with the legal regulations and guidelines applicable to your
range of application.
2. Switch off the device and disconnect it from the power supply.
3. Wipe down all parts of the device and accessories, including the connecting cable, with the disinfectant.
4. Clean the device with a mild soap-based household cleaning agent (see Cleaning on p. 73).
If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer
for disposal please note the following:
11 Troubleshooting
11.1 General errors
Technical faults may be triggered by faults such as a power failure or power fluctuations. In most cases it is
therefore sufficient simply to switch the device off and back on again after about 10 seconds. If the fault
occurs again, please note the fault code (e.g. 0x0B04) and inform Eppendorf Service.
Use the supplied packing material when packing the device for transport or storage. If you do not have this
anymore or if it is damaged, contact your Eppendorf office.
Proceed as follows:
1. Pay attention to the decontamination notes (see Decontamination before shipment on p. 76).
2. Switch off the device and open the heated lid.
3. Let the device stand for 30 minutes and ensure that there is no condensate in the thermoblock.
4. Disconnect the device from the power supply.
5. Disconnect all the connections from the rear of the device and remove the control panel.
6. Load the thermoblock with an empty PCR plate.
7. Close the heated lid.
8. Pack the device into the plastic bag.
9. Place the foam bottom part onto the bottom of the packaging.
10. Hold the device at the front and back of the lower part of the housing and lift it into the foam bottom
part.
11. Place the foam lid on top of the device.
12. Close the carton lid and insert the four plastic locking clips into the cut-outs on the side of the carton.
13. For fastening, push in the flaps of the plastic locking clips.
12.2 Transport
Use only the original packaging for transport.
The transport and storage conditions are described separately(see Ambient conditions on p. 83).
Transport, storage and disposal
Mastercycler pro 81
English (EN)
12.3 Disposal
In the event of disposing of the product, please observe the applicable legal regulations.
Information on the disposal of electrical and electronic devices in the European Community
The disposal of electrical devices is regulated within the European Community by national regulations
based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).
In accordance with this, any devices delivered after 13/08/2005 on a business-to-business basis, which
includes this product, may no longer be disposed of in household waste. To document this they have been
marked with the following identification:
As disposal regulations may differ from one country to another within the EU please contact your supplier,
if necessary.
Transport, storage and disposal
82 Mastercycler pro
English (EN)
Technical data
Mastercycler pro 83
English (EN)
13 Technical data
13.1 Power supply
13.2 Weight/dimensions
14 Ordering Information
14.1 Mastercycler pro
14.2 Accessories
14.3 Consumables
The twin.tec PCR plates are also available in various frame colors (red, yellow, green, blue). The complete
range of PCR consumables can be found in the Eppendorf catalog or at www.eppendorf.com.
Index D
Date + Time .......................................................... 58
1
Decontamination................................................... 76
1 … 3 StepCyc (program step) ..............................44
Define administrator PIN...................................... 31
Desinfection .......................................................... 76
A
Disposal ................................................................ 81
Access rights .........................................................55
Address display .....................................................29
E
Administrator functions.........................................55
Export ............................................................. 62, 62
Alarm Program listing................................................ 47
Activate ............................................................58
Deactivate ........................................................58
F
Auto-Restart ..........................................................60
fast (header setting).............................................. 43
Auto-update of the control panel software............63
Folder
Import.............................................................. 62
B
Folders
Backup...................................................................62 Changing the comment ................................... 39
Changing the name ......................................... 39
Beeper ...................................................................58
Copy ................................................................ 40
block (header setting)............................................43 Delete .............................................................. 40
New ................................................................. 39
C
G
Change country settings........................................58
Gradient .......................................................... 41, 46
Changing the date .................................................58
Guest
Changing the time .................................................58
User ................................................................. 34
Cleaning
Heated lid.........................................................74
H
Housing............................................................74
Heated lid
Confirmation..........................................................57
Address display ............................................... 29
Connect the control panel Cleaning position............................................. 74
Connecting multiple cyclers ............................23 Close ................................................................ 27
Connecting the mouse .....................................26 Control temperature (manually) ...................... 60
to the Mastercycler pro....................................23 Open ................................................................ 28
Status lamp...................................................... 29
Connecting the mouse...........................................26
Switch lid heater off automatically.................. 43
Connecting the printer ..........................................26 Temperature control before program start...... 43
Control Panel Hold (program step) ............................................. 44
Keys..................................................................30
Holding time increment........................................ 41
Country Settings....................................................58
Index
Mastercycler pro 89
English (EN)
I Deactivate........................................................ 58
Define .............................................................. 31
Impulse..................................................................43
Program
Incubate.................................................................60
Change comment ............................................ 40
Continue .......................................................... 53
K Copy ................................................................ 40
Delete .............................................................. 40
Keys .......................................................................30
Edit header ...................................................... 42
Export .............................................................. 62
L Import.............................................................. 62
Interrupt .......................................................... 53
Log file
New ................................................................. 39
Display .............................................................58
Rename............................................................ 40
Export...............................................................58
Restore ............................................................ 62
Login .....................................................................33 Save ................................................................. 62
Start ................................................................. 51
Logout ...................................................................34
Stop ................................................................. 53
Transfer from the Mastercycler 533x .............. 64
M Write protection .............................................. 40
Mains connection ..................................................22 Program editor
Description ...................................................... 41
Mini-satellite system
Editing parameters .......................................... 45
Networking ......................................................13
Exit .................................................................. 47
Set-up...............................................................23
Export program listing .................................... 47
MMC Extended options............................................. 45
Insert ................................................................37 Header ............................................................. 42
Labware ...........................................................38 Insert step........................................................ 44
Use ...................................................................37 Open ................................................................ 41
Save program .................................................. 47
Set gradient ..................................................... 46
N
Set holding time increment ............................. 46
Navigation tree ......................................................35 Set temperature control speed ........................ 46
Set temperature increment ............................. 46
Networking
Connecting Mastercycler pro to the Program header
Mastercycler ep................................................25 Define cycler type............................................ 42
Mini-satellite system ..................................13, 23 Impulse............................................................ 43
PC control ........................................................13 Set lid temperature.......................................... 42
Simulate Mastercycler gradient ...................... 43
n StepCyc (program step)......................................44
Switch off lid at low block temperature .......... 43
Temp. mode..................................................... 43
P TSP/ESP heated lid.......................................... 43
Pause (program step) ............................................44 Protocol
Display............................................................. 53
PDF........................................................................38
Print................................................................. 54
PIN Save ................................................................. 54
Activate ............................................................58
Administrator ...................................................31
Change .............................................................57
Index
90 Mastercycler pro
English (EN)
R Thermoblock
Calibration and adjustment ............................. 60
Restart ...................................................................60
Control temperature (manually) ...................... 60
Restore ..................................................................62 Equipment ....................................................... 50
Sample volume ................................................ 50
Self test............................................................ 61
S
Validate............................................................ 60
safe (header setting)..............................................43
Time increment..................................................... 46
Safety instructions
TXT ....................................................................... 38
Meaning of symbols...........................................7
Sample tubes .........................................................49
U
Sample volume ......................................................50
Unpacking............................................................. 21
Select Location ......................................................22
Update control panel software.............................. 63
Self test..................................................................61
USB
Show system memory ...........................................58 Connecting a storage medium ........................ 37
Connecting the printer .................................... 26
Simulate Mastercycler 5333/5331.........................43
Ports ............................................................... 25
Software Removing the storage medium ....................... 38
Synchronization ...............................................59 Use storage media........................................... 37
Update control panel .......................................63
User
Update Cycler ..................................................59
Change ............................................................ 33
Sound (program step) ...........................................44 Login ............................................................... 33
Logout ............................................................. 34
Space requirements...............................................22
User accounts
Status lamp............................................................29
Access rights ................................................... 55
Status view ............................................................52 Create .............................................................. 55
Delete .............................................................. 56
Switch ESP on and off ...........................................43
Edit .................................................................. 56
Switch lid heater off automatically........................43
User log
Switch off device ...................................................54 Display............................................................. 59
Export .............................................................. 59
Switch TSP on and off ...........................................43
Exporting and importing ................................. 59
System memory.....................................................58
System settings .....................................................58
T
Temp (program step).............................................44
Temp. mode...........................................................43
Temperature control speed .............................41, 46
Temperature increment...................................41, 46
Text file format ......................................................38
Índice
Mastercycler pro 91
Español (ES)
Índice
Manual
Mastercycler
ver
en
Tab.
Fig.
pág. de instrucciones
Español
Manual proinstrucciones
de
(ES)
3 Manejo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
3.1 Primeros pasos . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
3.1.1 Utilizar la tapa térmica vapo.protect. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
3.1.2 Teclas del panel de control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
5 Mantenimiento . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
5.1 Limpieza . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
5.1.1 Limpieza de la carcasa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
5.1.2 Limpieza de la tapa térmica . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
5.2 Desinfección/descontaminación . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Índice
92 Mastercycler pro
Español (ES)
Descripción del producto
Mastercycler pro 93
Español (ES)
1 2 34
7
8
11
9
10
1 2 3 4 5 6 7
1
2
3
4
Conecte únicamente aparatos que correspondan a las normas IEC 950/EN 60950 (UL 1950) en las
interfaces del Mastercycler pro y del panel de control.
Instrucciones generales de seguridad
Mastercycler pro 95
Español (ES)
El Mastercycler pro está previsto para el uso en laboratorios de investigación y de trabajos de rutina de las
ciencias de la vida.
El Mastercycler pro sirve para la temperación de soluciones, suspensiones o emulsiones acuosas dentro de
tubos de reacción cerrados para reacciones encimáticas, típicamente para reacciones en cadena de la
polimerasa (PCR).
Este aparato sólo está previsto para ser utilizado por personal especializado debidamente formado.
Lea atentamente las instrucciones de uso y familiarícese con el modo de funcionar del aparato antes de
trabajar con él por primera vez.
En los siguientes casos pueden verse mermadas las garantías previstas para el aparato. Entonces, la
responsabilidad del funcionamiento del aparato recae en el operador:
Antes de que utilice el Mastercycler pro, debe leer primero las instrucciones de uso y observar las
siguientes indicaciones generales de seguridad.
Instrucciones generales de seguridad
96 Mastercycler pro
Español (ES)
1
2
1 ¡ADVERTENCIA!
Peligro de quemadura a causa del termobloque (triángulo
con la punta hacia abajo) o a causa de la parte interior
caliente de la tapa calefactora (triángulo con la punta hacia
arriba).
En caso de funcionamiento con gradientes, la temperatura
en el lado derecho es superior a la del lado izquierdo.
2 ¡ADVERTENCIA!
Peligro de quemaduras provocadas por superficies
calientes.
Manejo
Mastercycler pro 101
Español (ES)
3 Manejo
El vapo.protect dispone de una unidad de presión flexible calentable. Al cerrar la tapa térmica, la unidad de
presión se coloca sobre las superficies de los tubos de muestras de manera ligera. A continuación se
aumenta la presión en el interior de un cojín de líquido dentro de la unidad de presión, la cual garantiza
una presión uniforme de los tubos contra el termobloque y asegura las tapas de los tubos contra eventuales
fugas. A través de la calefacción se evita que se forme condensado en la parte superior de los tubos durante
el calentamiento del líquido de reacción.
Requisitos
El termobloque del Mastercycler pro ha sido equipado con tubos de ensayo o con una placa PCR.
1. Sujetar la tapa térmica del asa y jalarla vapo.protect hacia adelante hasta dar con el tope, de manera que
el asa de la tapa se pueda girar ahora.
2. Plegar el asa por encima de la tapa térmica hasta que encaje.
Usted ahora puede ejecutar un programa o efectuar un calentamiento manual de la tapa térmica y de sus
muestras.
Abata el asa hacia arriba por encima de la tapa térmica y empuje la tapa hacia atrás hasta el tope
sujetándola del asa.
Manejo
Mastercycler pro 103
Español (ES)
La tapa térmica vapo.protect está provista de una luz indicadora de estado 2 (ver Ilustración general en
pág. 93). Así usted también podrá reconocer el estado de funcionamiento actual del Mastercycler pro desde
más lejos.
La tapa térmica vapo.protect está provista de una indicación de dirección de dos dígitos para facilitar la
identificación de los termocicladores (ver Ilustración general en pág. 93).
1 2 3
abc def
Stop
4 5 6
ghi jkl mno
7 8 9
pqrs tuv wxyz
Start
0 . Delete
Enter
Next
Teclas de función
La función cambia con el diálogo del software y siempre aparece en la indicación
directamente encima de la tecla.
Manejo
104 Mastercycler pro
Español (ES)
4 Acceso rápido
4.1 Login
1. Abrir la lista.
Enter
2. Seleccionar el usuario.
1 2
abc
3
def
5. Introducir el código PIN y
4 5 6 confirmarlo.
ghi jkl mno
7 8 9
pqrs tuv wxyz
0 . Delete
Enter
Acceso rápido
106 Mastercycler pro
Español (ES)
1 2
abc
3
def
3. Introducir un nombre y un
4 5 6 comentario, y confirmarlo luego.
ghi jkl mno
7 8 9
pqrs tuv wxyz
0 . Delete
Enter
1. Seleccionar el programa.
7 8 9
pqrs tuv wxyz
0 . Delete
1 2 3
abc def
4 5 6
ghi jkl mno
7 8 9
pqrs tuv wxyz
0 . Delete
6. Confirmar la entrada.
Enter
5 Mantenimiento
5.1 Limpieza
Limpie las superficies del Mastercycler pro con un paño húmedo y libre de pelusas. En caso necesario
utilice un producto de limpieza de laboratorio neutral.
Las instrucciones para la desinfección y descontaminación del aparato están especificadas por separado
(ver Desinfección/descontaminación en pág. 114).
2. Presionar hacia arriba (1) las bridas, que se encuentran debajo del borde delantero de la tapa a derecha
e izquierda, y soltar el bloqueo.
3. Plegar la tapa térmica hacia arriba (2) hasta que el soporte de la tapa encaje.
Mantenimiento
Mastercycler pro 113
Español (ES)
3
4
4. Eliminar la suciedad en la zona del bloque y en el lado inferior de la tapa con un paño húmedo y libre de
pelusas. En caso necesario utilizar un producto de limpieza de laboratorio. Restos de la rotulación en
tapas de envases sobre la unidad calefactora se pueden eliminar con etanol o isopropanol.
Mantenga las perforaciones del bloque libres de polvo y pelusas.
Pequeñas cantidades de deposiciones ya pueden mermar la transmisión de calor entre el
bloque y los tubos de ensayo.
5.2 Desinfección/descontaminación
¡PELIGRO! Descarga eléctrica debido a la penetración de líquidos.
Apague el aparato y desenchúfelo de la alimentación eléctrica antes de empezar los
trabajos de limpieza o de desinfección.
No deje que ningún líquido penetre en el interior de la carcasa.
No realice ninguna desinfección por pulverización.
Sólo vuelva a enchufar el aparato a la toma de corriente cuando este esté completamente
seco.
¡ATENCIÓN! Daños materiales por radiación UV y otro tipo de radiación rica en energía.
No realice una desinfección por irradiación de rayos UV, beta y gamma, ni con ningún otro
tipo de radiación rica en energía.
1. Seleccione un método de desinfección que concuerde con las normas y disposiciones legales válidas
para el respectivo campo de aplicación.
2. Apagar el aparato y desconectarlo de la alimentación eléctrica.
3. Limpiar por frotación todas las piezas del aparato, de los accesorios así como del cable de conexión con
el desinfectante.
4. Limpiar el aparato utilizando un producto de limpieza de uso doméstico suave a base de jabón (ver
Limpieza en pág. 111).
Evaluate your manual