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    CompactDryNissuiXSA IllustrationManual

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    Kesmavet UPTDLKK

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    © All Rights Reserved
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    CompactDryNissuiXSA IllustrationManual

    Uploaded by

    Kesmavet UPTDLKK
    35 views8 pages

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    CompactDryNissuiXSA-IllustrationManual

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    Copyright:

    © All Rights Reserved
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    35 views8 pages

    CompactDryNissuiXSA IllustrationManual

    Uploaded by

    Kesmavet UPTDLKK

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 8

    CompactDry “Nissui” X-SA

    Illustration Manual

    Copyright © Nissui Pharmaceutical Co., Ltd. All rights reserved.


    Weigh 50g solid sample

    and add 450mL Buffered Sodium Chloride


    Peptone Solution to the sample.
    It is recommended to use a stomacher bag
    with filter to eliminate risks of carry over of
    tiny pieces of foodstuffs into the surface of
    the medium.

    Homogenize this mixed sample by a


    blender
    Copyright © Nissui Pharmaceutical Co., Ltd. All rights reserved. 2
    Open aluminum bag, and take out a set of 4 plates.

    Copyright © Nissui Pharmaceutical Co., Ltd. All rights reserved. 3


    Take off the cap of the plate

    Write the appropriate information on


    the memorandum section.

    Copyright © Nissui Pharmaceutical Co., Ltd. All rights reserved. 4


    Pipette 1ml of homogenized specimen
    (to be further diluted if necessary) in
    the middle of dry sheet of Compact
    Dry X-SA.

    Specimen diffuses automatically and


    evenly into all over the sheet (total
    medium of 20 cm2) to transform it
    into gel within seconds.
    Copyright © Nissui Pharmaceutical Co., Ltd. All rights reserved. 5
    Viable count in swab
    test sample

    Inoculate 1 ml of
    wiping solution (to be
    diluted if necessary),
    which is obtained from
    cotton swab,
    Copyright © Nissui Pharmaceutical Co., Ltd. All rights reserved. 6
    Turn over the plate capped

    put in an incubator.
    Incubate for 24 + 2 hours at 37+ 1 ℃.

    Copyright © Nissui Pharmaceutical Co., Ltd. All rights reserved. 7


    Staphylococcus aureus forms light blue/blue
    colonies of 1~2 mm in diameter.

    Detection limit of Compact Dry X-SA is


    between 1 – 300 cfu/plate.

    From backside of the plate, count the number of


    colored colonies appeared in the medium.
    White paper placed under the plate can help to
    count colonies easier.
    Copyright © Nissui Pharmaceutical Co., Ltd. All rights reserved. 8

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