Es14-00387 1
Es14-00387 1
Es14-00387 1
Citation: Mateo-Sánchez, M. C., N. Balkenhol, S. Cushman, T. Pérez, A. Domı́nguez, and S. Saura. 2015. Estimating
effective landscape distances and movement corridors: comparison of habitat and genetic data. Ecosphere 6(4):59. http://
dx.doi.org/10.1890/ES14-00387.1
Abstract. Resistance models provide a key foundation for landscape connectivity analyses and are
widely used to delineate wildlife corridors. Currently, there is no general consensus regarding the most
effective empirical methods to parameterize resistance models, but habitat data (species’ presence data and
related habitat suitability models) and genetic data are the most widely used and advocated approaches.
However, the practical consequences of applying one or the other approach have not been well studied. To
address this knowledge gap, we performed a comparative study on the implications of using habitat
suitability versus genetic data for determining effective landscape distances (a proxy inversely related to
isolation among patches) based on least-cost and circuit-theoretic approaches, and for identifying potential
movement corridors. For our comparison, we used data for the Cantabrian brown bear in Spain, an
endangered population for which connectivity has been identified as a major conservation concern. Our
results show that for brown bears, habitat models tend to overestimate resistance to movement through
non-optimal areas, whereas genetic data yield higher estimates of effective distances within habitat areas.
Therefore, our results suggest that (1) dispersal might be generally less constrained by landscape
conditions than habitat utilization in home ranges, and that (2) dispersing animals might be more flexible
in their movement behavior than residents are in their habitat resource utilization behavior, with records
for residents dominating species occurrence data and subsequent habitat models. The assessed approaches
provided dissimilar connectivity models with notable differences in patterns of predicted corridors across
the study area, mainly due to differences in predicted connections between subpopulations. Our results
highlight that the functional differences in habitat vs. genetic data, as well as the assumptions and potential
limitations of different analytical approaches that use these data, need to be considered more carefully in
connectivity modeling and subsequent corridor delineation.
Key words: circuit theory; connectivity; dispersal; habitat suitability; landscape genetics; least-cost paths; resistance.
Received 16 October 2014; revised 2 December 2014; accepted 5 December 2014; final version received 4 February 2015;
published 21 April 2015. Corresponding Editor: D. P. C. Peters.
Copyright: Ó 2015 Mateo-Sánchez et al. This is an open-access article distributed under the terms of the Creative
Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
the original author and source are credited. http://creativecommons.org/licenses/by/3.0/
E-mail: mc.mateo@upm.es
(Nores and Naves 1993, Schwartz et al. 2003). in Spain, its peripheral areas and the belt area
Males and females have intra and inter-sexually between the two subpopulations. As shown in
overlapping home ranges (Dahle and Swenson Fig. 1, both subpopulations occupy a similar area
2003) and dispersal primarily occurs by males, of about 2,500 km2 each and are separated by
while females typically are philopatric (Swenson about 50 km of unoccupied range.
et al. 1998). Brown bear is highly dependent on
large landscapes with low human-footprint and Landscape resistance parameterization
large extents of forest cover (Clevenger et al. Landscape resistance was parameterized un-
1997, Apps et al. 2004, Mateo-Sánchez et al. der three different scenarios based on different
2014a). The brown bear population in the methodological approaches (habitat scenario,
Cantabrian Range suffered a dramatic decline genetic-multiplicative scenario, and genetic-ad-
in the last several centuries as a result of human ditive scenario), as described next. All resistance
persecution and progressive loss and fragmenta- maps were produced with a spatial resolution of
tion of its habitat (Naves et al. 2003). We 100 m.
considered this population highly suited for this Habitat suitability as a proxy for resistance to
analysis for the following reasons. (1) In the movement.—A plausible way to empirically esti-
Cantabrian Range, brown bears occur in two mate relationships between connectivity and
small, apparently isolated and endangered sub- environmental conditions is to assume that
populations, with about 220 individuals in total habitat quality has a direct (inverse) relationship
(Pérez et al. 2014). Connectivity has been with resistance to movement (e.g., Pullinger and
identified as a major conservation concern for Johnson 2010, Kuemmerle et al. 2011, Mateo-
this species, with potentially large implications Sánchez et al. 2014b). We used this approach in
for actual planning and conservation measures in our first parameterization scenario (habitat sce-
the study area (Ballesteros and Palomero 2012). nario), in which we created a resistance surface
(2) Brown bears and other large mammals are of where resistance to movement was obtained
particular interest for connectivity networks through an inverse function of habitat suitability.
because these species operate at broad scales We used a multiscale suitability habitat model
and occur at low densities, which imply that their developed by Mateo-Sánchez et al. (2014a) to
populations are more likely to be affected by the predict brown bear occurrence in the study area.
loss of connectivity (Beier et al. 2008). (3) Large After transformation every pixel represented the
amounts of habitat and genetic data are available unit cost of crossing each location, so that the
for the species and recent research has focused on lowest resistance value represented the cost of
landscape resistance and connectivity for this moving through the highest quality habitat.
species (Pérez et al. 2009, 2010, Mateo-Sánchez et Landscape variables included in the model were
al. 2014a, b) which provides a unique opportunity landscape composition (percentage of landscape
for the comparative analyses needed to effective- covered by forest), forest canopy cover and
ly tackle the aforementioned objectives. density of buildings (Mateo-Sánchez et al.
2014a, b).
MATERIALS AND METHODS Landscape genetics to infer resistance to move-
ment.—We used individual-based landscape ge-
Study area netics approaches to produce resistance models
The study was carried out in the Cantabrian under two different scenarios (Mateo-Sánchez et
Range (northwestern Spain). This area is within a al., in press). Specifically, we used genetic samples
larger transnational initiative covering protected of brown bears genotyped at 17 polymorphic
areas from the Cantabrian Range to the Western microsatellite loci to quantify genetic structure
Alps (SW Europe), in which previous studies on and measured the genetic distance among
connectivity and the barrier effect of roads for samples as the proportion of shared alleles
forest mammals have focused (Gurrutxaga et al. (Bowcock et al. 1994). The relationship between
2011, Jongman et al. 2011). The region is 49,472 the genetic structure observed within the bear
km2 in extent and contains the whole known population and likely drivers of landscape
range of the native populations of the brown bear resistance was systematically evaluated through
Fig. 1. Maps showing the location of the study area in Spain (small map) as well as the distribution of brown
bears within the study area in Spain (large map).
reciprocal causal modeling (Cushman et al. 2006, bear locations distributed across the species
2013b) and the multi-model optimization ap- range for which we also counted with genetic
proach developed by Shirk et al. (2010). The data, which allowed meaningful comparisons
resulting resistance models included variables of between results of the different approaches
landscape composition (percentage of landscape (habitat vs. genetic).
cover by mixed forest and agricultural lands), We applied two different approaches: (A) least
landscape configuration (cohesion of mixed cost path modeling, using the UNICOR software
forest and shrubland) and canopy cover. (Landguth et al. 2012), in which the movement of
One of the key questions in resistance param- individuals is assumed to follow the optimal
eterization relates to the method for combining (least costly) pathway between locations and (B)
the effects of individual landscape variables into circuit theory, using CIRCUITSCAPE (v3.5.8;
a multivariate resistance surface (Beier et al. McRae and Beier 2007, McRae et al. 2008), in
2008). Hence, we explored two different ways for which multiple available pathways (including
combining individual landscape variables: suboptimal ones) can be followed by the indi-
through multiplication (genetic-multiplicative viduals and contribute to estimated connectivity
scenario) and through addition (genetic-additive among locations. These two approaches were
scenario). used to (1) produce corridors expected to
concentrate brown bear movements in the study
Corridor identification and effective area (least cost path density and current density
distance estimation map for approaches A and B, respectively;
We used an individual-based approach to further details on our analyses can be found in
predict expected movement corridors. We con- Appendix A), and to (2) calculate the accumu-
sidered as sources and destinations for the lated cost of movement between source and
corridor mapping a set of 173 empirical brown destination areas, corresponding to the so called
Fig. 2. Locations for effective distance and effective resistance calculations. We calculated effective distances
and resistances between (a) the cores of the ranges of the two subpopulations, (b) the periphery of the two ranges,
and (c) within the two ranges. See also Table 1.
effective distances in least cost path modeling resistance scenarios described above. Since we
and to effective resistances (or resistance dis- aimed to compare the effective distances pro-
tance) in circuit-based modeling (hereafter both duced by the three resistance scenarios, each with
referred to as effective distances). Higher effec- different ranges of variation in the resistance
tive distances among locations are assumed to values, we first normalized effective distances by
correspond to a higher degree or likelihood of dividing the effective distance in each scenario by
isolation among habitat areas or locations. the mean resistance value of all the 173 pixels
Effective distances were calculated between (a) with bear locations in the corresponding resis-
the western subpopulation core area and eastern tance surface.
subpopulation core area, (b) the western sub- We therefore produced and compared six sets
population peripheral area and eastern subpop- of corridors and effective distance/resistance
values, corresponding to the two analytical
ulation peripheral area (i.e., the edge of each core
approaches (least cost paths and circuitscape)
population area that is closest to the other
and the three resistance surface scenarios (Table
subpopulation edge) and (c) end to end within
2).
each subpopulation area (i.e., travel through the
whole occupancy area) (Fig. 2). We focused on
RESULTS
these positions due to their strategic significance
in terms of connectivity between and within the Corridor comparison
two subpopulations (see also Table 1). These The predicted movement corridor network
linkages (corridors) and effective distances were among individuals showed substantial differenc-
produced for each of the three landscape es across the three resistance scenarios and two
Table 2. Sets of methods being compared for delineating corridors and estimating effective distances. These
methods are based on three different approaches for parameterizing resistance models, and two different
approaches for delineating corridors and estimating effective distances based on these models.
Resistance parameterization
Corridor delineation
approach Habitat scenario Genetic-multiplicative scenario Genetic-additive scenario
Least-cost path density Corridor option 1 Corridor option 2 Corridor option 3
(Fig. 3a) (Fig. 3b) (Fig. 3c)
Circuit theory Corridor option 4 Corridor option 5 Corridor option 4
(Fig. 4a) (Fig. 4a) (Fig. 4c)
analytical approaches (Figs. 3 and 4). Important- for effective resistances based on circuit theory.
ly, major functional links did not match among However, effective distances between strategic
scenarios or methods (least cost path vs circuit locations showed considerably different values
theory). In the case of linkages defined through among scenarios (Fig. 5). Effective distances
cumulative density of least cost paths on a between two individuals located within different
resistance map derived from habitat suitability, centers of the two subpopulations were 7–9%
connections between subpopulations followed lower for genetic scenarios (multiplicative and
two main routes that converged in the peripheral additive) than for the habitat scenario. More
area of the East subpopulation (Fig. 3a). In importantly, when two individuals were located
contrast, for the resistance scenarios based on in the proximate peripheral areas (edges) of both
genetic data and least cost path analysis, connec- subpopulations, effective distance was much
tions showed more extensive networks consisting higher for the habitat scenario than for genetic
in one major route complemented by several scenarios (47% and 79% higher for the genetic-
secondary routes that converge with the princi- multiplicative and genetic-additive scenarios,
pal route when using multiplicative genetic respectively). When comparing the multiplicative
resistance (Fig. 3b). In the additive genetic and additive genetic scenarios, the normalized
resistance scenario analyzed with least cost effective distances were more similar than when
paths, three parallel and interconnected routes comparing the habitat and genetic scenarios, but
coalesce in both subpopulations (Fig. 3c). When the additive genetic scenario showed the lowest
analyzing connections designated through circuit effective distances between individuals. In con-
theory, potential connections identified through trast, effective distance between two individuals
current maps also differed across the assessed located in the same core in both subpopulations
resistance scenarios. A higher concentration of was 40–30% lower when calculated across
current in narrower and more clearly defined resistance based on habitat suitability than when
areas is found in the habitat based resistance computed for resistance scenarios based on
scenario (Fig. 4a). When resistance was based on genetic data. Effective distances within cores
genetic data, more and wider permeable areas were relatively similar in the two genetic
were detected (Fig. 4b, c), with movements less scenarios, but the multiplicative approach
concentrated in thin strips of land. Results also showed higher values.
show that within-subpopulation connectivity Effective distances (effective resistances) calcu-
network pattern was relatively similar for all lated through circuit theory followed the same
the scenarios in both approaches. general pattern as least-cost effective distances
when considering movements between popula-
Effective distances comparison tion edges and movements within populations
There were strong correlations between effec- (Fig. 5b). In these cases, effective resistances in
tive distances across the three resistance scenar- the genetic-multiplicative and genetic-additive
ios assessed when all pairs of locations where scenarios were again considerably lower than for
simultaneously considered: Pearson’s correlation the habitat-based resistance scenario (37% and
coefficient r . 0.96 in all the cases for effective 51%, respectively). Oppositely, effective resistanc-
least cost distances and r . 0.82 in all the cases es between the centers of both subpopulations
Fig. 3. Corridors defined by using least-cost paths for the three resistance scenarios: (a) Habitat suitability-
based scenario, (b) genetic-multiplicative scenario, and (c) genetic-additive scenario.
Fig. 4. Current maps defined by circuit theory for resistance scenarios: (a) Habitat suitability-based scenario, (b)
genetic-multiplicative scenario, and (c) additive genetic-based scenario.
DISCUSSION
Most current methods to predict population
connectivity and to identify areas important for
animal movements rely on landscape resistance
surfaces (Spear et al. 2010). However, the
methods and assumptions used to create these
surfaces are critical for their effectiveness in
guiding conservation decisions. Therefore, it is
important to explore the effects that different
analytical approaches have on predicted popula-
tion connectivity and identified corridor routes.
Here, we compared resistance surface parame-
terization and resulting connectivity models
created by two different analytical approaches
for quantifying resistances and two commonly
used methods to predict likely movement paths Fig. 5. Comparison of normalized effective distances
from these resistances. Our findings showed that among resistance scenarios. Distances are shown for
alternative approaches substantially affected the the two analytical approaches used (a) least cost paths
assessment of effective distances and largely and (b) circuit theory. Resistance scenarios are: habitat-
changed the delineation of potential corridors suitability based, genetic-multiplicative and genetic-
across the study area. additive. Between subpopulations centers corresponds
to the normalized effective distance between two
Implications of multiplicative vs. individuals located in the core of the west subpopu-
additive combination of factors lation and the core of the east subpopulation; between
The way in which factors are combined in a subpopulation corresponds to the normalized distance
resistance model has important implications for between two individuals located in the peripheral area
resulting connectivity models. Our additive of the west subpopulation and in the peripheral area of
resistance surface showed denser connectivity the east subpopulation (border of each area closest to
networks and lower effective distances/resistanc- the other subpopulation); within west and east
subpopulations corresponds to the normalized effec-
es than the multiplicative surface. This suggests
tive distance between the opposite edges of that
that multiplicative resistance models are more
subpopulation.
restrictive in identifying permeable areas for
movement and hence indicate lower connectivity.
This is because areas predicted to be highly allows compensation among factors and can lead
permeable in a multiplicative model correspond to less restrictive connectivity predictions.
to those raster cells where all the factors involved
in the models have low resistance values. Thus,
Comparison of least cost-path and
in a multiplicative model, even a single factor
with high resistance in an area can decrease circuit theory predictions
predicted landscape permeability in that area. In Least-cost path analysis and circuit theory are
contrast, an additive combination of factors based on different assumptions to model con-
nectivity (Spear et al. 2010). While least-cost identified through genetic-based resistances. In
analyses assume animals follow a single and addition, effective distances between subpopula-
optimal pathway, circuit theory assumes random tions were substantially higher when habitat
walks so that movement is influenced by all suitability was used as a surrogate for landscape
possible pathways. Therefore, results from both resistance. These findings suggest that resistance
methods provide different and complementary surfaces based on habitat models may tend to
insights about brown bear movement. For both overestimate landscape resistance in areas with
approaches, effective distances between subpop- low habitat suitability. In our analysis, this effect
ulation centers (the individual has to cross part of was dramatic when effective distance was calcu-
the habitat area and the matrix in-between lated between peripheral areas of both subpop-
subpopulation ranges) differed from effective ulations and individuals had to cross mostly
distances between subpopulation edges (the unsuitable areas. In contrast, effective distances
individual only crosses the matrix between between individuals located within subpopula-
habitat areas). However, and surprisingly, we tions were lowest when resistance surfaces were
found that circuit-theoretic effective resistance based on habitat suitability. Thus, congruent with
between subpopulation edges was actually high- results from Wasserman et al. (2010), we found
er than the effective resistance between popula- that habitat suitability can predict a greater
tion centers in the habitat-based resistance resistance to poor habitat than is suggested by
scenario (Fig. 5b). To have a lower effective genetic data. In other words, the fact that the
resistance between the centers of the habitat species does not occur ( permanently) in a
areas than between the less distant edges of those particular habitat does not imply that it cannot
areas is rather difficult to interpret ecologically,
move when needed (e.g., dispersal) through this
because bears moving away from the edges first
area. In this sense, landscape genetics models
have to reach the edges before crossing the
integrate the movement of many individuals
matrix to reach the other subpopulation. This
over time and thus lead to a more synoptic
result might point out to a potential limitation of
measure of landscape resistance (Zeller et al.
circuit theory (or of the implementation of it) to
2012, Cushman et al. 2013a). This suggests that
assess landscape connectivity. We ensured that
there is not necessarily a correspondence be-
our finding was not an artifact arising from some
tween habitat use patterns and dispersal move-
idiosyncratic and unnoted characteristic of our
particular brown bear spatial data by creating a ments. Conditions providing suitable habitat for
simple and purposefully controlled example permanent establishment and local resource may
depicting a resistance pattern similar to the one often be different than the conditions facilitating
of the habitat-based scenario (i.e., similar extent, dispersal movements (Cushman et al. 2013a,
resolution and arrangement). This controlled Peterman et al. 2014). Since suitability models
example confirmed our results from the real bear are based on occurrence data that usually
data set, i.e., effective resistances were smaller represent locations within home ranges, habitat
between the cores than between the edges suitability models may not adequately reflect
(further details can be found in Appendix A how environments affect animals during move-
and Appendix B: Fig. B1). This finding advocates ments outside of their usual home ranges, such
for further research to fully clarify this issue from as dispersal or mating excursions (Cushman et al.
an analytical point of view and, if possible, to 2013a). For our study species, results suggests
provide guidelines for avoiding potentially un- that dispersing bears are more flexible in their
intended results in the application of circuit movement behavior and less constrained by
theory for connectivity analyses. landscape conditions than suggested from their
occurrence in typical bear habitats. While our
Habitat models may overestimate resistance to analyses have only been conducted for a single
movement through non-optimal areas species, we believe that the results may be similar
Corridors between subpopulations predicted for many other organisms as well, particularly
from habitat suitability-based resistance did not for species that are highly mobile and not strictly
match the locations or intensity of corridors confined to a specific type of habitat.
Genetic data estimate higher resistance to per generation can lead to a sufficient amount of
movement through suitable habitat areas gene flow between populations (e.g., Mills and
For within-population movements, the genet- Allendorf 1996, Vucetich and Waite 2000). There-
ically-derived resistance surfaces predicted larger fore, facilitating sufficient levels of inter-popula-
effective distances among individuals than the tion movements should be a priority in
habitat suitability-based resistance surface. This connectivity strategies. Importantly, it is precisely
is likely due to the fact that resistance models in this kind of movements where, according to
derived from landscape genetic analyses are our results, resistance estimates and connectivity
based on comparison of genetic distances and models varied most strongly between the ana-
effective distances. Such landscape genetic ap- lytical approaches we compared. Within the two
proaches may lead to resistance surfaces that subpopulations, effective distances and resistanc-
overestimate effective distances in areas that are es were always low, and predicted movement
highly suitable (and eventually well connected to paths were very consistent among the different
each other) when factors other than resistance to analytical approaches. However, estimated dis-
movement govern spatial-genetic structure; for tances and predicted movement paths differed
instance, even when two sampled individuals are substantially among the different approaches for
separated by a very short distance (or even connectivity between the two subpopulations.
located in the same raster cell), they will show Thus, analytical choices for parameterizing and
some genetic differentiation, which may translate utilizing resistance surfaces will be most impor-
in such genetic resistance models exacerbating tant when trying to predict inter-population
the effective distance estimates at short ranges to movements, when individuals move out of
explain such genetic structure. Spatial-genetic established ranges and cross the unsuitable
structure within populations is likely less depen- landscape matrix. Importantly, this is also the
dent on the resistance of the landscape matrix kind of analysis most relevant for corridor
(Fahrig 2007), but more strongly influenced by design, as the goal is usually the (re-) connection
many other biological and ecological factors of different subpopulations located across a
acting locally, including sex-specific space-use landscape or the support of species range shifts
behavior, local population density, survival, or in response to climate change, rather than the
reproductive success (Pflüger and Balkenhol facilitation of within-population movements.
2014). Another issue related to genetic data is
that in long-lived species there may be a Conclusions for connectivity analysis based
temporal disconnection between genetic struc- on habitat suitability
ture and the current landscape; i.e., there may be Based on our study, it seems reasonable to
legacy effects of previous landscapes (James et al. question the assumption that habitat suitability
2007, Spear et al. 2010) that could lead to models can accurately capture landscape resis-
misestimate current connectivity. However, tance to movement for corridor design. Location
Landguth et al. (2010) showed that the legacy data used to produce habitat suitability models
of past landscape features is not a particularly tend to be dominated by habitat use (i.e., shelter,
important problem in species with relatively foraging) and thus by frequent routine move-
large dispersal abilities. Hence, landscape lega- ments within established home ranges. However,
cies are unlikely to affect our conclusions about genetic structure may be strongly determined by
brown bear dispersal and gene flow. mating movements and rare dispersal events.
These types of movements are unlikely to be
The importance of inter-population movements well-captured by occurrence data and may be
for predicting connectivity therefore poorly represented in resistance models
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frequent than within-habitat movements, but suggested by Beier et al. (2008) and Zeller et al.
they are also critical for genetic exchange and (2012), genetic-based studies are likely to be more
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SUPPLEMENTAL MATERIAL
APPENDIX A
DETAILS ON CORRIDOR IDENTIFICATION tween all combinations of sources and destina-
ANALYSES tions.
APPENDIX B
DETAILS ON THE EXAMPLE OF CIRCUITSCAPE centre to centre effective resistance was lower
ESTIMATES OF EFFECTIVE RESISTANCES than edge to edge (see Fig. B1), confirming our
findings from the brown bear data set. This
The simulated example consists of two habitat suggests that there might be a previously
areas with low resistance to movement that are
unreported issue related to some conceptual
separated by a high resistance matrix. We
computed circuit-based effective resistances be- aspects of circuit theory or its application to
tween two points located in the center of both model landscape connectivity, or issues related to
habitat areas and two points located on their details of the implementation of the calculations
closest edges (Fig. B1). Results showed that in Circuitscape.
Fig. B1. Simulated landscape for illustrating the results of the effective resistances through circuit theory as
implemented in Circuitscape. The landscape consists of two habitat areas with low resistance to movement
(resistance score 10) that are separated by a portion of landscape matrix with a high resistance (resistance score
100). Effective resistance was calculated (1) between two points located in the center of both habitat areas (shown
in magenta in the figure), resulting in 137.1 and 57.5 Ohms, respectively, for a four- and eight-neighbors case and
(2) between two points located in the habitat area edges (border of one habitat area closest to the other habitat
area, shown in blue in the figure), resulting in 162.3 and 69.84 Ohms, respectively, for a four- and eight-neighbors
case.