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    Active Ingredient in Detergent

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    Utkarsh Kadam
    This document summarizes a method for determining the active ingredient in detergent samples. The method involves titrating a solution of the detergent sample against a cationic detergent. The endpoint is detected visually when sufficient color change occurs between the aqueous and chloroform layers. The method was verified to have good precision between analysts, with reproducibility RSD below 3%. The limit of quantification was determined to be below 0.1% and measurement uncertainty was calculated to be ±0.30% at an obtained value of 16.5% active ingredient.

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    Active Ingredient in Detergent

    Uploaded by

    Utkarsh Kadam
    59 views2 pages
    This document summarizes a method for determining the active ingredient in detergent samples. The method involves titrating a solution of the detergent sample against a cationic detergent. The endpoint is detected visually when sufficient color change occurs between the aqueous and chloroform layers. The method was verified to have good precision between analysts, with reproducibility RSD below 3%. The limit of quantification was determined to be below 0.1% and measurement uncertainty was calculated to be ±0.30% at an obtained value of 16.5% active ingredient.

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    This document summarizes a method for determining the active ingredient in detergent samples. The method involves titrating a solution of the detergent sample against a cationic detergent. The endpoint is detected visually when sufficient color change occurs between the aqueous and chloroform layers. The method was verified to have good precision between analysts, with reproducibility RSD below 3%. The limit of quantification was determined to be below 0.1% and measurement uncertainty was calculated to be ±0.30% at an obtained value of 16.5% active ingredient.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    59 views2 pages

    Active Ingredient in Detergent

    Uploaded by

    Utkarsh Kadam
    This document summarizes a method for determining the active ingredient in detergent samples. The method involves titrating a solution of the detergent sample against a cationic detergent. The endpoint is detected visually when sufficient color change occurs between the aqueous and chloroform layers. The method was verified to have good precision between analysts, with reproducibility RSD below 3%. The limit of quantification was determined to be below 0.1% and measurement uncertainty was calculated to be ±0.30% at an obtained value of 16.5% active ingredient.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    DACPL, NAVI MUMBAI

    Document No. DACPL/NMUM/CHEM/VER/016 Determination of Active Ingredient in Detergent


    Performed Date. 18.10.2022
    Page No…1
    Job/Sample No… IQC 120

    Scope This method is applicable for Active ingredient in the range of 0.1--30%m/m.
    Method IS 4956 : 2020
    Principle A solution of anionic detergent containing added methylene blue is shaken in chloroform. The
    mixture is titrated with a cationic detergent. The end point is taken when sufficient methylene
    blue has been displaced into the aqueous layer to produce phases of equal colour intensity
    Reagents Sr Standard Make
    No.
    1 Sodium Lauryl Sulphate Qualigens
    2 Sodium Carbonate AASHVI
    3 Potassium hydrogen phthalate Merck

    Apparatus CALIBRATED INSTRUMENTS/EQUIPMENT /CONSUMABLE USED FOR VALIDATION


    Sr Item ID NO
    No.
    1 Analytical balance NMUM/CHE/BAL-009
    2 Burette DACPL/NMUM/CHEM/10ml/BR-2
    3 Pipette ACPL/NMUM/CHEM/10ml/GP-1

    Procedure Determination of purity of sodium lauryl sulphate

    1. Weigh around 0.9--1 g of sample & make up to 100ml with distilled water.
    2. Take 10 ml sample solution in 100 ml cylinder.
    3. Add 15 ml chloroform & 25 ml methylene blue reagent (0.005 %) to cylinder
    4. Shake well , the chloroform layer (lower) shall be coloured blue or greenish blue
    5. Add from burette Benzalkonium Chloride (0.004 M) slowly
    6. After each addition stopper the cylinder and shake well and allow to phases separates
    7. Initially chloroform layer will be blue or greenish blue, towards the end the colour would
    start migrating to the aqueous layer.

    Calculations:--

    Active Ingredient,%(m/m) = 342 X V2 X M2 X 5

    m2
    where
    342 = molecular mass of sodium alkyl benzene sulphonate taken for calculation

    V2 = volume of benzethonium chloride solution added, in ml

    M2 = molarity of benzethonium chloride solution


    PERFORMANCE CHARACTERISTIC

    Prepared By: Verified By:


    DACPL, NAVI MUMBAI
    Document No. DACPL/NMUM/CHEM/VER/016 Determination of Active Ingredient in Detergent
    Performed Date. 18.10.2022
    Page No…2
    Job/Sample No… IQC 120

    Precision Reproducibility was carried out using the same method on same test material in the same lab using the
    same equipment by different chemist.

    REPRODUCIBILITY

    Sr Operator Active Ingredient,% RSD % Acceptance Criteria, %RSD


    no (m/m)
    1 Chemist 1 16.3 1.2 3.0
    16.4
    2 Chemist 2 16.4
    16.6
    3 Chemist 3 16.2
    16.7
    Average 16.4
    Std. Deviation 0.2
    Limit of LOQ < 0.1 %(m/m)
    Quantification
    Measurement Measurement of uncertainty was calculated on the basis of Obtained Value of Active
    Of ingredient All the data attached.
    Uncertainty
    16.5 %m/m ± 0.30

    Statement Method Verification is ensured that the Standard Method used for Determination of Active
    ingredient in Detergent can achieve the required performance

    Reference AOAC INTERNATIONAL 21st Edition, 2019 (Appendix K)

    Prepared By: Verified By:

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