Exp. 11 Enzymes
Exp. 11 Enzymes
Exp. 11 Enzymes
Biochemistry Laboratory
EXPERIMENT NO. 11
ENZYMES
Objective(s): To prepare enzymes and be familiar with its physical and chemical
properties.
Theory:
Enzymes are complex organic compounds with definite chemical
structure, secreted by living things. They have the property of initiating and
hastening chemical reactions without themselves being affected in the process.
Enzyme activity is influenced by concentration of the enzyme, concentration of the
substrate, and concentration of the products of the reaction, temperature, pH,
inorganic salts and the presence of inhibitors and activators. All these factors
therefore should be considered while performing the following experiment.
Procedure:
Cut thin slices of either apple, guava, or chico and expose them to the air.
Observe the darkening of the exposed sliced sides. Explain the color change.
Observations?
B. Catalase:
PREPARATION:
1. Divide the filtrate into four parts and boil one part for 1 minute.
NOTE: The action of peroxidases depends to some extend upon phenol oxides which
decomposes the hydrogen peroxide. Potato has both peroxides and phenol oxides. This
accounts for the rapidity of actions in the presence of hydrogen peroxide, H2O2.
1. Take two 5 mL of starch solution and transfer them in each test tube.
5. Remove the solutions from the water bath then add Fehling’s reagent. Then
continue heating them in the water bath
Observations:
Questions:
2. What is a substrate?
3. What are the different kinds of catalyst and define each type.