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    Activity 13 Analysis of Saliva: Materials

    Uploaded by

    Liane Bartolome
    Saliva contains components that can help diagnose health conditions. The document outlines procedures to isolate and test for various components in saliva, including carbohydrates, proteins, nitrates, thiocyanate, glucose, inorganic phosphate, and chloride. Qualitative tests are performed using reagents like Benedict's solution, copper sulfate, iron chloride, silver nitrate, and ammonium molybdate to observe color changes indicating the presence of different ions and molecules in saliva. The results are summarized in a table to identify which substances are present based on the test observations.

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    Activity 13 Analysis of Saliva: Materials

    Uploaded by

    Liane Bartolome
    469 views2 pages
    Saliva contains components that can help diagnose health conditions. The document outlines procedures to isolate and test for various components in saliva, including carbohydrates, proteins, nitrates, thiocyanate, glucose, inorganic phosphate, and chloride. Qualitative tests are performed using reagents like Benedict's solution, copper sulfate, iron chloride, silver nitrate, and ammonium molybdate to observe color changes indicating the presence of different ions and molecules in saliva. The results are summarized in a table to identify which substances are present based on the test observations.

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    Original Title

    LAB_13-Saliva (1)

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    Saliva contains components that can help diagnose health conditions. The document outlines procedures to isolate and test for various components in saliva, including carbohydrates, proteins, nitrates, thiocyanate, glucose, inorganic phosphate, and chloride. Qualitative tests are performed using reagents like Benedict's solution, copper sulfate, iron chloride, silver nitrate, and ammonium molybdate to observe color changes indicating the presence of different ions and molecules in saliva. The results are summarized in a table to identify which substances are present based on the test observations.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    469 views2 pages

    Activity 13 Analysis of Saliva: Materials

    Uploaded by

    Liane Bartolome
    Saliva contains components that can help diagnose health conditions. The document outlines procedures to isolate and test for various components in saliva, including carbohydrates, proteins, nitrates, thiocyanate, glucose, inorganic phosphate, and chloride. Qualitative tests are performed using reagents like Benedict's solution, copper sulfate, iron chloride, silver nitrate, and ammonium molybdate to observe color changes indicating the presence of different ions and molecules in saliva. The results are summarized in a table to identify which substances are present based on the test observations.

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    © All Rights Reserved
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    of 2

    ACTIVITY 13

    ANALYSIS OF SALIVA

    Theory: Saliva, like urine, is a good barometer of metabolic activity, since both are
    derived from plasma. For this reason, analysis of the usual and unusual components of saliva
    may help in the diagnosis of pathogenic conditions in an individual

    MATERIALS

    Saliva sample (NH4)2MoO4 Litmus paper


    3M HCl 5% AgNO3 Flask
    3M NaOH Acetone Beaker
    1N HAc Benedict’s solution Funnel evaporating dish
    0.5 N HCl 2.5M NaOH Test tubes
    1% KI-starch solution 0.05% CuSO4 solution
    0.1M FeCl3 Filter paper

    PROCEDURES

    1. Isolation of mucin from saliva


    Rinse the mouth with water and collect 15 mL of saliva. Add 3 drops of 1N acetic acid
    and mix. To 30 mL of acetone, in an Erlenmeyer flask, add the saliva slowly shaking the
    flask after each addition. Stopper the flask and set aside for 30 minutes. Filter and wash
    the precipitate formed, using two 3 mL portions of acetone. Allow the precipitate to drain
    completely. Remove the filter paper from the funnel, spread it out in a watch glass and
    allow the precipitate to dry. Use the precipitated mucin to test for the possible
    components of saliva.( in 2a and 2b)

    2. Qualitative tests for the components of saliva


    a. Carbohydrates
    Place about ½ of the isolated mucin in a test tube. Add 1 mL of 3M HCl and
    immerse in a boiling water bath for 30-minutes.. Cool and add 3 drops of 3M NaOH,
    then add 3 mL of Benedict’s solution. Heat again in a boiling water bath for 10
    minutes.
    Observation:
    ___________________________________________________________

    b..Proteins
    Dissolve the remaining portion of mucin in 1 mL of 2.5M NaOH, then add 3 drops of
    0.05% CuSO4 solution. Mix.
    Observation:_________________________________________________________
    __

    II.Nitrates
    a.Mix 1 mL of. 0.05 N H2SO4, 2 drops of 1% KI and 2 drops of starch solution. Add 5
    mL of saliva. Mix.
    Observation:_________________________________________________________
    _

    b.Thiocyanate
    To 5 mL of saliva, add 5 drops of freshly prepared 0.1M FeCl3 solution and a 5
    drops of 0.1 N HCl.
    Observation:_________________________________________________________
    _

    c.glucose
    Using a saliva sample collected 15 minutes after eating, add 2 mL of Benedict’s
    solution to
    5 mL of saliva, then heat in a boiling water bath for 10 minutes.
    Observation:_________________________________________________________
    _

    d.Inorganic Phosphate
    Mix 5 mL of saliva and 2 mL of ammonium molybdate solution. Heat for 5 minutes,
    then add 10 drops of ammonium hydroxide.heat for 5 minutes more.
    Observation:_________________________________________________________
    _

    e.Chloride:
    Mix 5 mL of saliva with 5 drops of nitric acid followed by 5 drops of AgNO3 solution.
    Observation:_________________________________________________________
    _

    What ion is present in saliva based from your results in II a,b,d,e.

    RESULTS AND DISCUSSION


    Summarize your results in the table below:

    Substances tested Test reagents Results Conclusions


    for
    Carbohydrates NaOH, Benedicts
    soln, heat
    Proteins NaOH, CuSO4
    Nitrate H2SO4, KI, starch
    CNS- FeCl3, HCl
    Glucose Benedict’s soln,
    heat
    Inorganic Ammonium
    phosphate molybdate
    Chloride HNO3, AgNO3

    GENERALIZATION

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