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    BANING Activity No. 5.2 DNA Base Pairing

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    Fatima Nur Faiza Handa
    The document discusses the base pairing rules for DNA, RNA, and their transcription and translation. It provides examples of writing the complementary DNA strand, mRNA strand, tRNA sequence, and translating the amino acid sequence for given nucleic acid strands. Key rules covered include: A pairs with T, C pairs with G, and in RNA A pairs with U instead of T. The genetic code is based on mRNA codons, with each codon encoding a specific amino acid.

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    BANING Activity No. 5.2 DNA Base Pairing

    Uploaded by

    Fatima Nur Faiza Handa
    66 views3 pages
    The document discusses the base pairing rules for DNA, RNA, and their transcription and translation. It provides examples of writing the complementary DNA strand, mRNA strand, tRNA sequence, and translating the amino acid sequence for given nucleic acid strands. Key rules covered include: A pairs with T, C pairs with G, and in RNA A pairs with U instead of T. The genetic code is based on mRNA codons, with each codon encoding a specific amino acid.

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    The document discusses the base pairing rules for DNA, RNA, and their transcription and translation. It provides examples of writing the complementary DNA strand, mRNA strand, tRNA sequence, and translating the amino acid sequence for given nucleic acid strands. Key rules covered include: A pairs with T, C pairs with G, and in RNA A pairs with U instead of T. The genetic code is based on mRNA codons, with each codon encoding a specific amino acid.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    66 views3 pages

    BANING Activity No. 5.2 DNA Base Pairing

    Uploaded by

    Fatima Nur Faiza Handa
    The document discusses the base pairing rules for DNA, RNA, and their transcription and translation. It provides examples of writing the complementary DNA strand, mRNA strand, tRNA sequence, and translating the amino acid sequence for given nucleic acid strands. Key rules covered include: A pairs with T, C pairs with G, and in RNA A pairs with U instead of T. The genetic code is based on mRNA codons, with each codon encoding a specific amino acid.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    Name: Julia Marie A.

    Baning Section: BSMT 2D

    There are base pairing rules for writing complimentary DNA strands for a given strand.

    A pair with T
    C pairs with G
    In RNA, A pair with U, instead of T.

    Write the complimentary DNA strand for each given strand of DNA.

    1. CGT AAG CGC TAA TTA


    → GCA TTC GCG ATT AAT

    2. TCT TAA ATG ATC GAT C


    → AGA ATT TAC TAG CTA G

    3. AAT GAA TAG CTA GCT T


    → TTA CTT ATC GAT CGA A

    4. GGC ATT CGC GAT CAT G


    → CCG TAA GCG CTA GTA C

    5. CGT TAG CAT GCT TCA T


    → GCA ATC GTA CGA AGT A

    6. ACT AAC GGT A GCT AGC


    → TGA TTG CCA TCG ATCG

    Now write the mRNA strand for the given DNA strand.

    1. ATG TCG CTG ATA CTG T


    → UAC AGC GAC UAU GAC A

    2. GAA GCG ATC AGT TAC G


    → CUU CGC UAG UCA AUG C

    3. AAT GAA TAG CTA GCT T


    → UUA CUU AUC GAU CGA A

    4. GGC ATT CGC GAT CAT G


    → CCG UAA GCG CUA GUA C

    5. CGT TAG CAT GCT TCA T


    → GCA AUC GUA CGA AGU A

    6. ACT AAC GGT AGC TAG C


    → UGA UUG CCA UCG AUC G

    Write the tRNA sequence for the given strand of mRNA

    1. AGG UCA UGC AUG GGC AUG CAU


    → UCC AGU ACG UAC CCG UAC GUA

    2. AGA GAU UCA GCU AGC ACG AUA


    → UCU CUA AGU CGA UCG UGC UAU

    3. GUC AUC GAU CGA UCG GAU GCC


    → CAG UAG CUA GCU AGC CUA CGG

    4. UUU CAG UCA GCU AGC GAU CGU


    → AAA GUC AGU CGA UCG CUA GCA
    Now you will translate the amino acid sequence for the given tRNA strand. Remember that codons are 3
    base pairs long.

    1. AUG CAC UGU CCU UUC GCU GAC


    → Met His Cys Pro Phe Ala Asp

    2. GAG AUC UGG UUG GAA UCG


    → Glu Ile Trp Leu Glu Ser

    3. AGC GUA UUA ACG UAU CAU


    → Ser Val Leu Thr Tyr His

    4. AGU CGA UCG AUG CGG AUG AUA


    → Ser Arg Ser Met Arg Met Ile

    5. GUC GUC GAU AGC UAU CAU GCA


    → Val Val Asp Ser Tyr His Ala

    Transcribe the following DNA strand. Then translate the tRNA strand you wrote.

    1. TGA GTC GAC TGG CTG ACC GTA GAC


    → ACU CAG CUG ACC GAC UGG CAU CUG (mRNA)
    → UGA GUC GAC UGG CUG ACC GUA GAC (tRNA)
    → Thr Gln Leu Thr Asp Trp His Leu

    2. CTT GGC TTA TGG TGG TTC GCT CGC


    → GAA CCG AAU ACC ACC AAG CGA GCG (mRNA)
    → CUU GGC UUA UGG UGG UUC GCU CGC (tRNA)
    → Glu Pro Asn Thr Thr Lys Arg Ala

    The following are pieces of mRNA. Give the DNA strand from which it was transcribed.

    1. GAG AUC UGG UUG GAA UCG


    → CTC TAG ACC AAC CTT AGC

    2. AGC GUA UUA ACG UAU CAU


    → TCG CAT AAT TGC ATA GTA

    Complete the table below showing the sequences of DNA, mRNA codons, tRNA anticodons and the amino
    acids. Remember the genetic code is based on mRNA codons.

    DNA 1. ACC 2. TTG GAT 3. ATA

    mRNA codon 4. UGG 5. AAC 6. CUA UAU

    tRNA anticodon 7. ACC UUC 8. GAU 9. AUA

    Amino Acid Tryptophan 10. Asparagine 11. Leucine 12. Tyrosine

    1. Using the following piece of DNA, give the mRNA molecule and the amino acid sequence for which it
    codes.

    DNA – ATA TAA ACG AGG AAA TTC CGG GCG

    mRNA – UAU AUU UGC UCC UUU AAG GCC CGC

    tRNA – AUA UAA ACG AGG AAA UUC CGG GCG

    Amino acids: Tyr Ile Cys Ser Phe Lys Ala Arg
    2. Use the mRNA sequence to find the DNA sequence and the amino acid sequence.

    DNA – TAC GGA TGT ACA CCA CAT TGG AAT

    mRNA – AUG CCU ACA UGU GGU GUA ACC UUA

    tRNA – UAC GGA UGU ACA CCA CAU UGG AAU

    Amino acids: Met Pro Thr Cys Gly Val Thr Leu

    3. For each codon below, give the tRNA anticodon.

    1. UUC AAG
    2. AUC UAG
    3. CCG GGC
    4. CGU GCA

    4. Give all the possible Anti-codons for the amino acids listed below.

    Histidine (His) – CAU, CAC


    Isoleucine (Ile) – AUA, AUC, AUU
    Arginine (Arg) – AGG, AGA, CGG, CGA, CGC, CGU
    Tryptophan (Trp) – UGG

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