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    Acid Protase Trial

    Uploaded by

    Narinder Pal Singh Sokhey
    This document details several trials analyzing the properties of A-starch and A-starch slurries after various enzymatic treatments. Key parameters such as pH, temperature, color, odor, appearance, protein concentration and absorbance were measured at different enzyme dosage levels. The 0.5% dosage of acid protease increased the average protein concentration from 5.69 to 6.43 mg/ml. The 1.0% dosage further increased the average protein concentration to 13.23 mg/ml. Combined enzymatic treatments with Enzypap and acid protease at 1.25% dosage resulted in final protein concentrations of 6.94 mg/ml.

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    Acid Protase Trial

    Uploaded by

    Narinder Pal Singh Sokhey
    18 views6 pages
    This document details several trials analyzing the properties of A-starch and A-starch slurries after various enzymatic treatments. Key parameters such as pH, temperature, color, odor, appearance, protein concentration and absorbance were measured at different enzyme dosage levels. The 0.5% dosage of acid protease increased the average protein concentration from 5.69 to 6.43 mg/ml. The 1.0% dosage further increased the average protein concentration to 13.23 mg/ml. Combined enzymatic treatments with Enzypap and acid protease at 1.25% dosage resulted in final protein concentrations of 6.94 mg/ml.

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    This document details several trials analyzing the properties of A-starch and A-starch slurries after various enzymatic treatments. Key parameters such as pH, temperature, color, odor, appearance, protein concentration and absorbance were measured at different enzyme dosage levels. The 0.5% dosage of acid protease increased the average protein concentration from 5.69 to 6.43 mg/ml. The 1.0% dosage further increased the average protein concentration to 13.23 mg/ml. Combined enzymatic treatments with Enzypap and acid protease at 1.25% dosage resulted in final protein concentrations of 6.94 mg/ml.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Download as xlsx, pdf, or txt
    18 views6 pages

    Acid Protase Trial

    Uploaded by

    Narinder Pal Singh Sokhey
    This document details several trials analyzing the properties of A-starch and A-starch slurries after various enzymatic treatments. Key parameters such as pH, temperature, color, odor, appearance, protein concentration and absorbance were measured at different enzyme dosage levels. The 0.5% dosage of acid protease increased the average protein concentration from 5.69 to 6.43 mg/ml. The 1.0% dosage further increased the average protein concentration to 13.23 mg/ml. Combined enzymatic treatments with Enzypap and acid protease at 1.25% dosage resulted in final protein concentrations of 6.94 mg/ml.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Download as xlsx, pdf, or txt
    of 6

    Trial start Date: 15/05/2012 Day 1 Date:15/05/2012

    Primary Estimation of A-Starch


    S. no Parameters Analysed Values
    1 pH of A-Starch 5.85
    2 Temperature of A-Starch 21
    3 Colour of A-Starch Creamy white
    4 Odour of A-Starch Wheaty fermented
    5 Appearance of A-Starch highly Viscous liquid with high sedimentation
    6 Total Nitrogen content in A-Starch 0.224
    7 Total protein in A-Starch 1.28
    8 Dissolved Solids in A-Starch 54.76

    a A-starch & water ratio to prepare slurry 1:1


    b Dilution Factor 0.5
    Primary Estimation of A-Starch slurry
    S. no Parameters Analysed Values
    1 pH of A-Starch slurry 5.78
    2 Temperature of A-Starch slurry 20.7 Absorbance control sample
    3 Colour of A-Starch slurry Off white Blank 0.093
    4 Odour of A-Starch slurry Wheaty fermented 1 0.222 5.95
    5 Appearance of A-Starch slurry Semi-viscous with high sedimentation 2 0.216 5.68
    6 Total Nitrogen content in A-Starch slurry 0.117 3 0.216 5.68
    7 Total protein in A-Starch slurry 0.67 4 0.211 5.45
    8 Dissolved Solids in A-Starch slurry 30.87
    9 protein conc. Mg/ml 5.69 Mg/ml Avg 5.69

    a Batch size 500


    b Incubation time 90 min
    c Incubation Temperatur 50oC

    Final estimation after enzymatic treatment 0.5 %


    S. no Mode of application Enzyme status dose Color Odour Apperance protein conc. Mg/ml Absorbance
    1 Blank Sample Not Added Nil 0.095
    2 Acid Protease Added 0.50% 6.36 0.233
    3 Acid Protease Added 0.50% 6.36 0.233
    4 Acid Protease Added 0.50% 6.45 0.235
    5 Acid Protease Added 0.50% 6.58 0.238

    a K protein Slurry 1.22


    b K protein Solid 0.61 Pure starch after Supernatant

    Final estimation after enzymatic treatment 1.0 %


    S. no Mode of application Enzyme status dose Color Odour Apperance protein conc. Mg/ml Absorbance
    1 Blank Sample Not Added Nil 0.089
    2 Acid Protease Added 1.00% 13.16 0.378
    3 Acid Protease Added 1.00% 13.11 0.377
    4 Acid Protease Added 1.00% 13.34 0.382
    5 Acid Protease Added 1.00% 13.29 0.381

    a K protein Slurry 1.18


    b K protein Solid 1.14 Fiber
    Primary Estimation of A-Starch slurry
    S. no Parameters Analysed Values
    1 pH of A-Starch slurry 5.91
    2 Temperature of A-Starch slurry 25.3
    3 Colour of A-Starch slurry Off white
    4 Odour of A-Starch slurry Wheaty fermented
    5 Appearance of A-Starch slurry Semi-viscous with high sedimentation
    6 Total Nitrogen content in A-Starch slurry 0.121
    7 Total protein in A-Starch slurry 0.69
    8 Dissolved Solids in A-Starch slurry 27.52
    9 protein conc. Mg/ml 4.58

    a Batch size 500 ml


    b Incubation time 120 min
    c Incubation Temperature 50oC

    Final estimation after enzymatic treatment Enzypap 0.6%


    S. no Mode of application Enzyme status dose Color Odour
    1 Blank Sample Not Added Nil
    2 Enzypap Added 0.60%
    3 Enzypap Added 0.60%

    a K protein Slurry

    Final estimation after enzymatic treatment 1.0 % (Enzypap + Acid Pr


    S. no Mode of application Enzyme status dose Color Odour
    1 Blank Sample Not Added Nil
    2 Acid Protease+ Enzypap Added 1.00%
    3 Acid Protease+ Enzypap Added 1.00%

    a K protein Slurry
    b K protein Solid

    Final estimation after enzymatic treatment 1.25 % (Enzypap + Acid P


    S. no Mode of application Enzyme status dose Color Odour
    1 Blank Sample Not Added Nil
    2 Acid Protease+ Enzypap Added 1.25%
    3 Acid Protease+ Enzypap Added 1.25%

    a K protein Slurry
    b K protein Solid
    Absorbance control sample
    Blank 0.85
    1 0.183 4.55
    2 0.184 5.60

    atment Enzypap 0.6%


    Apperance protein conc. Mg/ml Absorbance
    0.85
    5.91 0.213
    5.95 0.214

    1.0 % (Enzypap + Acid Protease)


    Apperance protein conc. Mg/ml Absorbance
    0.86
    6.31 0.223
    6.09 0.218

    .25 % (Enzypap + Acid Protease)


    Apperance protein conc. Mg/ml Absorbance
    0.086
    6.90 0.236
    6.99 0.238
    protein conc. Mg/ml control sample

    blank 0.086
    1 0.236 6.90 6.9
    2 0.238 6.99 6.99

    6.94

    protein conc. Mg/ml 0.5 % Dosing

    blank 0.095
    1 0.233 6.36
    2 0.233 6.36
    3 0.235 6.45
    4 0.238 6.58

    6.43

    protein conc. Mg/ml1.0 % Dosing

    blank 0.089
    1 0.378 13.16
    2 0.377 13.11
    3 0.382 13.34
    4 0.381 13.29

    13.23

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