Bio 270 Lab Part I - As120

BIO270 / OKT 2021 -FEB 2022
UNIVERSITI TEKNOLOGI MARA (UiTM)
Branch Pahang Campus Jengka
BIO 270
ANIMAL & PLANT PHYSIOLOGY
Laboratory Part 1
Dissection of Rat
Student Name SITI AISHA QHAIRUNNISSA BINTI ROSLAN

Student ID 2020855836
Group/Class AS1203B
H/P Number 0199188269
Email Address 2020855836@student.uitm.edu.my
Lecturer Name DR. ENGKU AZLIN RAHAYU BINTI ENGKU ARIFF
FOR LECTURER
USE ONLY
Section Mark
Part I /40
1
BIO270 / OKT 2021 -FEB 2022
2
BIO270 / OKT 2021 -FEB 2022
Instructions:
1. This workbook must be completed and submitted individually.

2. Complete this workbook. The total marks of this workbook are 40 marks.
3. You are given links to several videos and website. Watch the videos and
website before answering the workbook questions.
4. You may use other reliable information to assist you when necessary.
5. Should you need any further clarification about the assignment, please consult
with your lecturer.
6. Answer ALL questions in this workbook. DO NOT alter this workbook, please
use the space given to write your answer accordingly.
7. After completing the workbook, each student must save his/her workbook in
pdf before submitting it to their respective lecturers via an online platform before the
due date.
8. Failure to submit this workbook may affect your continuous assessment
marks.
TOPIC LINK
How animals https://www.youtube.com/watch?v=U9OiEAWbU-M

1. are used in
research
2. Humane killing https://www.understandinganimalresearch.org.uk/animals

/three-rs/
https://youtu.be/2Du0h270a-c
3. Dissection https://youtu.be/9Xk2KCYTujk
procedure
4. Interview with https://youtu.be/ezt9p00PSOg

the Veterinarian
Carcass https://youtu.be/VDiGd4620ac
Disposal
3
BIO270 / OKT 2021 -FEB 2022
HOW ANIMALS ARE USED IN RESEARCH

1. From the video https://www.youtube.com/watch?v=U9OiEAWbU-M, name the
animal and state their importance in research. (6 marks)
Animal (0.5 mark each) Importance in Research (1 mark each)
- Mice are used to research into cancer, human

cancer can be given to mice.
Mice
- Zebrafish are used to study tuberculosis and
stroke.
Zebrafish
- Dogs lungs are similar to human lungs so dogs
are used to test asthma drugs.
Dog
- Monkeys are used to study vision because they
have binocular vision like humans.
Monkey
4
BIO270 / OKT 2021 -FEB 2022
HUMANE KILLING
1. After some procedures, it may be necessary to kill the animal to prevent
further suffering. The person who conducted the procedure must ensure that the
animal is killed competently and humanely. List out the appropriate methods used in
humane killing for the animals in research (2 marks). Refer to website
https://www.understandinganimalresearch.org.uk/animals/three-rs/
1) Replace the use of animals with alternative techniques, or avoid the use of animals
altogether.
2) Reduce the number of animals used to a minimum, to obtain information from fewer
animals or more information from the same number of animals.
3) Refine the way experiments are carried out, to make sure animals suffer as little as
possible. This includes better housing and improvements to procedure which minimise
pain and suffering and for improve animal welfare.
2. From the video https://youtu.be/2Du0h270a-c, what method is used to

sacrifice the animal? (1 mark)
The method that was used to sacrifice the animal was exposing the animal to Carbon Dioxide
gas.
5
BIO270 / OKT 2021 -FEB 2022
DISSECTION PROCEDURE
1. After viewing the video https://youtu.be/9Xk2KCYTujk, the dissected rat is
illustrated in Figure 1. Label the parts in the box provided below (10 marks)
Figure 1
Organ (1 mark each) Organ (1 mark each)

A Trachea F Large intestine
B Lung G Spleen
C Diaphragm H Stomach
D Liver I Heart
E Small intestine J Esophagus
2. From the video, what are the organs that were identified/observed BUT were
NOT shown/stated in the Figure 1 (4 marks).
1.Kidney 2. Urinary bladder 3. Uterus 4. Ovary
6
BIO270 / OKT 2021 -FEB 2022
Fill in the columns with the appropriate answers (8 marks).
Organ Main Components Main Function

System (1 mark each) (1 mark each )
Example: Heart, blood vessels, Internal distribution of materials
Circulatory blood such as O2, CO2, ions.
nose, mouth, throat, voice Deliver oxygen into the body and
Respiratory box, windpipe, and lungs. distribute or remove waste gases
including carbon dioxide.
mouth, esophagus, Motility, digestion, absorption and

Digestive stomach, small intestine, secretion are the four vital
large intestine, rectum functions of the digestive system.
and anus.
Ovaries, testes Gamete production, promotion of
Reproductive fertilization and support developing
embryo
Kidneys, ureters, urinary Disposal of metabolic wastes,

Excretory bladder, urethra regulation of osmotic
And balance of blood
3. Based on your understanding, identify the part of the body that can be used to
collect blood without killing the animal (3 marks).
1.Hand 2. Neck 3. Thigh
4. From the veterinarian surgeon point of view, https://youtu.be/ezt9p00PSOg.

list out a few challenges faced by a veterinarian (3 marks).
1) Handling wildlife animals or zoo because they are not being domesticated and not
clean. They can also be fierce and aggressive.
2) Facing animals that have many diseases such as cancer or tumour.
3) Animals that are fierce and aggressive.
7
BIO270 / OKT 2021 -FEB 2022
CARCASS DISPOSAL
1. The carcass must be disposed of properly. By referring to the video, describe
how you would dispose of the carcass in the lab (3 marks).
https://youtu.be/VDiGd4620ac
1) Firstly, the carcass needs to be put into a bio-hazard bag.

2) Then the bio-hazard bag needed to be sealed or tie tightly.
3) Then, the carcass needed to be stored in animal carcasses freezer. Make sure that the
temperature is set to -40℃ .
4) Recorded the details of user, date, time, number of carcasses of animal and the type of the
animals into the freezer book log.
5) Lastly, the carcasses will be taken by the clinical waste contractor every two weeks to be
collected and disposed of.
__________________________________________________________________________________
ACKNOWLEDGEMENT
We would like to thank all the teaching team and lecturers in charge of BIO270
Animals and Plants Physiology UiTM for their expertise and suggestions in ensuring
these assessment activities are a success. Here, we also acknowledge, with special
thanks, to Dr Nursakinah Latifi and Mr Muhammad Fadhli Mohd Azahar from
Laboratory Animal Facility and Management (LAFAM) as well as Madam Nina
Keterina Hashim from Faculty of Health Sciences, UiTM Puncak Alam for their
involvement and production of the video demo.
REFERENCES
https://www.youtube.com/watch?v=U9OiEAWbU-M
https://www.understandinganimalresearch.org.uk/animals/three-rs/
_________________________________
Thank You ________________________________

Bio 270 Lab Part I - As120

Uploaded by

Copyright:

Available Formats

Bio 270 Lab Part I - As120

Uploaded by

Document Information

Original Description:

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Bio 270 Lab Part I - As120

Uploaded by

Copyright:

Available Formats

BIO270 / OKT 2021 -FEB 2022

UNIVERSITI TEKNOLOGI MARA (UiTM)

Branch Pahang Campus Jengka

Student Name SITI AISHA QHAIRUNNISSA BINTI ROSLAN

1. This workbook must be completed and submitted individually.

How animals https://www.youtube.com/watch?v=U9OiEAWbU-M

2. Humane killing https://www.understandinganimalresearch.org.uk/animals

4. Interview with https://youtu.be/ezt9p00PSOg

HOW ANIMALS ARE USED IN RESEARCH

Animal (0.5 mark each) Importance in Research (1 mark each)

- Mice are used to research into cancer, human

2. From the video https://youtu.be/2Du0h270a-c, what method is used to

Organ (1 mark each) Organ (1 mark each)

1.Kidney 2. Urinary bladder 3. Uterus 4. Ovary

Fill in the columns with the appropriate answers (8 marks).

Organ Main Components Main Function

mouth, esophagus, Motility, digestion, absorption and

Kidneys, ureters, urinary Disposal of metabolic wastes,

1.Hand 2. Neck 3. Thigh

4. From the veterinarian surgeon point of view, https://youtu.be/ezt9p00PSOg.

1) Firstly, the carcass needs to be put into a bio-hazard bag.

You might also like