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    Act.1 RQ 2 Pipettes and Others 1-3

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    Francis Valdez
    This document discusses cleaning procedures for various laboratory equipment including pipettes, Sahli pipette, Pasteur pipette, suction canisters, forceps, staining racks, and glassware. Proper cleaning is important to avoid contamination and corrosion. Equipment should be rinsed with appropriate solvents and disinfectants followed by drying. Storage methods are also outlined to keep equipment clean between uses.

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    Act.1 RQ 2 Pipettes and Others 1-3

    Uploaded by

    Francis Valdez
    81 views3 pages
    This document discusses cleaning procedures for various laboratory equipment including pipettes, Sahli pipette, Pasteur pipette, suction canisters, forceps, staining racks, and glassware. Proper cleaning is important to avoid contamination and corrosion. Equipment should be rinsed with appropriate solvents and disinfectants followed by drying. Storage methods are also outlined to keep equipment clean between uses.

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    Act.1 RQ 2 pipettes and others 1-3

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    This document discusses cleaning procedures for various laboratory equipment including pipettes, Sahli pipette, Pasteur pipette, suction canisters, forceps, staining racks, and glassware. Proper cleaning is important to avoid contamination and corrosion. Equipment should be rinsed with appropriate solvents and disinfectants followed by drying. Storage methods are also outlined to keep equipment clean between uses.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    81 views3 pages

    Act.1 RQ 2 Pipettes and Others 1-3

    Uploaded by

    Francis Valdez
    This document discusses cleaning procedures for various laboratory equipment including pipettes, Sahli pipette, Pasteur pipette, suction canisters, forceps, staining racks, and glassware. Proper cleaning is important to avoid contamination and corrosion. Equipment should be rinsed with appropriate solvents and disinfectants followed by drying. Storage methods are also outlined to keep equipment clean between uses.

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    © All Rights Reserved
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    of 3

    PIPETTES

    RBC Thoma pipette and WBC Thoma pipette

    - The RBC pipette is identified by the red bead in the bulb, and the WBC pipette by the white bead
    in the bulb. Both are cleaned as follows. Attach the devices provided to the end of a pipette.
    Rinse (fill and empty) the pipette thoroughly by drawing and expelling a dilute bleach mixture
    (2.5%) several times. Repeat this procedure substituting distilled water as a rinse. Next, alcohol
    (95%) is used for two rinses. Finally, acetone or ether is used for two rinses. Air is then forced
    through the pipette until it is dry.

    Figure 2f from: Irimia R, Gottschling M (2016) Taxonomic revision of Rochefortia Sw. (Ehretiaceae,
    Boraginales). Biodiversity Data Journal 4: E7720. https://doi.org/10.3897/BDJ.4.e7720. (n.d.).
    Fundamentals of Hematology Cell Counts and Measurements. doi:10.3897/bdj.4.e7720.figure2f

    Storage
    - Store pipettes vertically to avoid contamination.
    - Store pipettes dialed to highest volume setting.
    - Store away from heat and moisture.
    - Pipette tips should be removed prior to storage.
    - Keep pipettes clean and free of contaminants
    - When cleaning pipettes with bleach solution, do not saturate as fluid can make its way to the
    piston and cause corrosion.

    Isobel Maciver https://www.promegaconnections.com/author/imaciver/. (2021, January 23). How to


    Take Care of Your Pipettes. Retrieved from https://www.promegaconnections.com/how-to-take-care-of-
    your-pipettes/

    Sahli Pipette

    - The Sahli calibrated graduated cylinder must be cleaned after each use by washing with soapy
    water and rinsing with clean water.
    • Storing the brown glass standard away from sunlight prevents the color from fading.

    Bangash, A (August 22, 2016) Sahli method Hemoglobin Estimation. Retrieved from
    https://medicalstudyzone.com/sahli-method/

    GUYS OPeN NIYO DIN TO HEHE


    https://path.azureedge.net/media/documents/TS_anemia_guide_health_workers.pdf

    Pasteur Pipette

    - You should clean and re-use your Pasteur pipets when possible. Do it immediately, before the
    chemical dries on the pipet and makes it impossible to clean. If the pipet has only been used for
    a solvent (pure hexanes,ether, ethanol, etc.), simply let it dry. If it has been used for a solution
    of a solid compound in a solvent, or for an aqueous acid or base, rinse it with the minimum
    amount necessary to get the bulk of the chemical off - use water or acetone - into the
    appropriate waste container, then rinse it fully under running water. Put it in your lab drawer
    and it will be ready for use at your next lab meeting.
     University of Colorado at Boulder, Department of Chemistry and
    Biochemistry.https://www.orgchemboulder.com/Technique/Procedures/Pipets/Pipets.shtml

    - Keep it clean between uses by storing it upright in clean glassware (such as a beaker or
    Erlenmeyer flask). If the pipet is broken or cannot be used again for any other reason, rinse any
    chemicals off (discard the rinse in the appropriate waste container) and then dispose of the
    pipet in the glass waste.

    Solano, D. M, n.d. Department of Chemistry & Biochemistry California State University, Bakersfield
    Retrieved From https://www.csub.edu/chemistry/organic/manual/Topic7_Pipet.pdf

    Improvised suction device

    - The suction canister should be emptied daily and washed, along with the connecting tubing, in
    hot water and mild dishwashing detergent. Rinse with clean, hot tap water. It is recommended
    that these items be disinfected daily with a solution of one part white vinegar and 3 parts water.
    Allow the items to soak for 30 minutes and then rinse with clean, hot tap water.

    Cleveland Clinic: Respiratory Theraphy at Home. Retrieved from


    https://my.clevelandclinic.org/ccf/media/files/Neurological-Institute/suction-machine-
    guidelines.pdf

    - 1. Gather your equipment and supplies:


    - one container filled with warm, soapy water (use mild dish-washing soap)
    - second container filled with one part white vinegar and three parts water
    - clean cloth
    - clean towel
    - 2. Remove the short tubing from the lid. Remove the canister, take the lid off, and empty the
    contents into the toilet.
    -
    - 3. Place all parts of the canister in the first container filled with warm, soapy water then rinse
    with tap water.
    -
    - 4.Place all parts of the canister in the second container filled with vinegar and water, soak for 30
    minutes, then rinse with tap water.
    -
    - 5. Place all parts on a clean, dry towel and allow to air dry.

    Airway Clearance (November 21, 2019). HINT: How do I clean and maintain a suction machine. Retrieved
    from https://www.connectedcare.sickkids.ca/quick-hits/how-do-i-clean-and-maintain-a-suction-
    machine
    Hematocrit Reader- guys wala gid ako may makita dito sorry 

    Forceps

    - Immediately after use, rinse instruments under warm or cool running water to remove all blood,
    body fluids and tissue. Dried soils may damage the instrument surface and make cleaning very
    difficult. Do not use hot water as this will coagulate proteinous substances.
    - Rinse instruments thoroughly under running water. While rinsing, open and close scissors,
    hemostats, needle holders and other hinged instruments to ensure that hinge areas are also
    rinsed. Dry instruments thoroughly with a clean towel. This minimizes the risk of corrosion and
    formation of water spots. Use spray lubricant in the hinges to improve function of instrument.

    World Precision Instruments n.d. Retrieved from https://www.wpiinc.com/blog/post/surgical-


    instrument-care-and-handling

    Staining rack

    - Wet your scrub brush or toothbrush with hot water, add a dab of soap, and gently scrub at
    stains to remove them. Make sure to scrub the entire rack thoroughly in addition to focusing on
    stains.

    Avantor. Staining Rack. Retrieved from https://us.vwr.com/store/category/staining-racks/568177

    - Rinse the glassware with the appropriate solvent. Use deionized water for water-soluble
    contents. Use ethanol for ethanol-soluble contents, followed by rinses in deionized water. Rinse
    with other solvents as needed, followed by ethanol and finally deionized water. If the glassware
    requires scrubbing, scrub with a brush using hot soapy water, rinse thoroughly with tap water,
    followed by rinses with deionized water.

    Eppendorf AG. Barkhausenweg 122339 Hamburg, Germany. Retrieved from


    https://www.labmanager.com/in-focus/automated-solutions-simplify-next-generation-sequencing-
    library-preparation-981

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