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    Science Experiment Banana Extract

    Uploaded by

    Ali Calizo
    1) The objectives of this experiment were to extract and observe banana DNA. Students would learn the proper procedure and materials needed. 2) The hypothesis was that dishwashing soap, a water and salt mixture, and rubbing alcohol added to mashed banana would help break cell membranes and walls to release DNA strands. 3) The required materials included banana, dishwashing soap, water, salt, rubbing alcohol, and various tools.

    Copyright:

    Public Domain
    Download as DOCX, PDF, TXT or read online from Scribd

    Science Experiment Banana Extract

    Uploaded by

    Ali Calizo
    126 views8 pages
    1) The objectives of this experiment were to extract and observe banana DNA. Students would learn the proper procedure and materials needed. 2) The hypothesis was that dishwashing soap, a water and salt mixture, and rubbing alcohol added to mashed banana would help break cell membranes and walls to release DNA strands. 3) The required materials included banana, dishwashing soap, water, salt, rubbing alcohol, and various tools.

    Original Description:

    Extraction of banana

    Original Title

    SCIENCE EXPERIMENT BANANA EXTRACT

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    © Public Domain

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    1) The objectives of this experiment were to extract and observe banana DNA. Students would learn the proper procedure and materials needed. 2) The hypothesis was that dishwashing soap, a water and salt mixture, and rubbing alcohol added to mashed banana would help break cell membranes and walls to release DNA strands. 3) The required materials included banana, dishwashing soap, water, salt, rubbing alcohol, and various tools.

    Copyright:

    Public Domain
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    126 views8 pages

    Science Experiment Banana Extract

    Uploaded by

    Ali Calizo
    1) The objectives of this experiment were to extract and observe banana DNA. Students would learn the proper procedure and materials needed. 2) The hypothesis was that dishwashing soap, a water and salt mixture, and rubbing alcohol added to mashed banana would help break cell membranes and walls to release DNA strands. 3) The required materials included banana, dishwashing soap, water, salt, rubbing alcohol, and various tools.

    Copyright:

    Public Domain
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 8

    EXPERIMENT OBJECTIVES:

    At completion of this activity, we will:

     Know how to successfully extract DNA from a banana, given the


    materials provided and procedure.

     Able to see the DNA of Banana by extracting it.

     Have the opportunity to use prior and newly acquired knowledge to


    draw conclusions regarding the structure and function of DNA.

    HYPOTHESIS:

    The three (3) items / materials that added to the mashed banana
    will help to break cells apart to get the DNA from it.

     Dishwashing liquid soap


    It can break a certain type of molecule that makes
    membranes around cells and nucleus that holds DNA, which
    is the lipids.
     Mixture of water and salt
    Once the dishwashing soap release the DNA, this mixture
    will help the DNA strands to stick together for us to see it
    even without using microscope.
     Rubbing Alcohol
    Same with the mixture of water and salt, this will help to
    clumps the DNA.

    MATERIALS:
    What do we need?
    2. Bottled drinking water 3. Salt
    5. Rubbing alcohol 6. Measuring tools
    8. Coffee filter 9. Plastic fork
    1 2 3
    11. Glass vial 12. Coffee stirrer
    13. Ice cube 14. Plastic cups

    4 5 6

    7 8 9

    10 11 12

    1. Banana
    4. Dishwashing liquid
    soap
    7. Stainless steel fork
    10. Plastic Container
    SET-UP / PREPARATIONS:

    We mushed the banana using blender


    for about a minute until all the lumps
    are gone and it almost looks like
    pudding

    We filled the glass vial with 15ml


    Isoprophyl alcohol and put it in the
    plastic cup with ice cube on it and
    let it cool.

    Prepare the materials and


    follow the given Procedures.
    PROCEDURES:

    How we do it?
    1

    First, we added ½ cup bottled drinking water in the mashed


    banana and we set it aside.

    1 teaspoon salt, 1 teaspoon dishwashing liquid soap and 2


    tablespoons of water - we mixed it together in a plastic cup and
    we gently stirred it for us to make sure no foam will produce.
    3

    After we mixed it, we also added 2 tablespoons of banana mixture


    and we stirred it again for about 10 minutes using the plastic fork.

    Next, we placed the coffee filter on the top of the plastic cup and wet
    the middle of it using the drinking water. Afterwards we removed the
    water from the cup and put back the wet coffee filter and we
    proceed on pouring the mixture we made into it.
    5

    Then, we slowly poured the filtrate (the collected liquid from the bottom
    of the cup) to the vial with cold alcohol.

    Lastly, we set it aside and waited until white precipitate formed (DNA).
    [AFTER
    [Failed attemptsSEVERAL TIMES
    = 3 times. (Foam formed OFin ATTEMPT]
    the mixtures; ripped
    coffee filter)]
    Observation: Precipitate formation (DNA) at the top of the mixture.

    CONCLUSIONS:

    Through this activity, we, the students, informally assessed our


    ability to follow directions / procedures. We learned also that we don’t
    just read and follow the instructions but also we need to understand it. In
    this activity as well, we must not hurry things up because like what
    happened on our attempts, we keep on making mixture again and again
    due to lack of softness in stirring the mixture. However, as we realized
    our mistakes during the experiment, we were able to succeed and see
    our expected result, which was to see the DNA of the banana.
    “TRY AND TRY UNTIL YOU SUCCEED.”

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