F4bio P3 2019

SULIT 2
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Answer all questions.
1. Most fruits contain ascorbic acid normally known as vitamin C.

Vitamin C is a strong reducing agent for Dichlorophenolindophenol (DCPIP).
A group of students carried out an experiment to determine the concentration of vitamin C in

fruit juices using DCPIP solution. Diagram 1.1 shows the initial volume of 0.1% of ascorbic
acid solution and Diagram 1.2 shows the initial volume of fruit juice used in this experiment.
Diagram 1.1 Diagram 1.2
The students carried out the following steps:
Step 1:
1 ml of 0.1 % DCPIP solution is placed in a beaker.
Step 2:
5 ml of 0.1% ascorbic acid solution is filled into a 5 ml syringe with needle. 0.1% ascorbic
acid is added drop by drop to the DCPIP solution until the solution becomes colourless.
Step 3:
Record the volume of 0.1% ascorbic acid solution used in Table 1 on page 3.
Step 4:
Steps 1 – 3 are repeated by using papaya juice, guava juice and mango juice.
Table 1 shows the result of the experiment.

Type of solution/ Final reading of syringe Volume of solution/ fruit juice
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to decolourise 1 ml of 0.1%
fruit juice (ml)
DCPIP solution (ml)
0.1% ascorbic
acid solution
Papaya juice
Guava juice
Mango juice
Table 1
For a) Record the volume of 0.1 % ascorbic acid solution of fruit juices to decolourise 1
Examiner’s ml of 0.1% DCPIP solution in Table 1 on page 3. [3
use

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marks]
1(a)
b) (i) Based on Table 1, state two different observations.
3
Observation 1:
Observation 2:
1(b)(i)
3 [3 marks]
(ii) State two inferences which correspond to the observations in 1(b)(i).
Inference from observation 1:
Inference from observation 2:

1(b)(ii)
3 [3 marks]
c) Complete Table 2 based on the experiment.
Variable Method to handle the variable
Manipulated variable
____________________________ __________________________________
____________________________ __________________________________
____________________________ __________________________________
Responding variable
____________________________ __________________________________
____________________________ __________________________________
____________________________ __________________________________
Constant variable
____________________________ __________________________________
1(c) ____________________________ __________________________________
____________________________ __________________________________
3
Table 2 [3 marks]
For d) State the hypothesis for the experiment.
Examiner’s
use
1(d)
[3 marks]
3

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e) (i) Construct a table and record all the data collected from the experiment.
Your table should have the following titles:
 Type of solution or fruit juices
 Volume of solution or fruit juices used to decolourise 1 ml of 0.1% DCPIP
solution
 Concentration of vitamin C
Use the formula:
Volume of 0.1 % ascorbic acid
Concentration of vitamin C= ×1.0 mg /ml
Volume of fruit juice
1(e)(i)
[3 marks]
1(e)(ii) (ii) Using the graph paper provided to answer this question.
Using the data in 1(e)(i), draw a bar chart of concentration of vitamin C
3 against the types of fruit juices.
[3 marks]
f) Based on the bar chart drawn in 1(e)(ii), state the relationship between the
concentration of vitamin C and types of fruit juices.
Explain your answer.
1(f)
[3 marks]
3
For g) Another group of students carried out the same experiment by using processed
Examiner’s mango juice as shown in Diagram 1.3.
use

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Diagram 1.3
Predict the volume of processed mango juice that need to be used to decolourise the
DCPIP solution. Explain your answer.
1(g)
3 [3 marks]
h) Based on the result from this experiment, state the operational definition for
vitamin C.
1(h)
[3 marks]
3
i) The following list are fruit that contain vitamin C.
Banana Apple Blackcurrant Pineapple
Classify the fruits into high content of vitamin C and low content of vitamin C in
Table 3.
1(i)
High content of vitamin C Low content of vitamin C
3
Total
1
Table 3
33 [3 marks]
2. Cellular respiration is the process of oxidising food molecules, like glucose. Carbon dioxide
and water are the products of this process.
C6H12O6 + 6 O2 → 6 H2O + 6CO2
5(b)(i)
Inhale air is actually the surrounding air than contains approximately 21% oxygen, 0.04%
carbon dioxide and 79% nitrogen. Exhale air contains approximately 16% oxygen, 4% carbon
1
dioxide and also 79% nitrogen. The only difference between them is the varying levels of
oxygen and carbon dioxide.
Diagram 2 shows an apparatus used in determining the amount of certain gas in air sample.

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Diagram 2
By using materials and apparatus available in the laboratory, plan a laboratory experiment to
investigate the effect of duration of a vigorous activity on the amount of carbon dioxide
released in the exhale air.
Your experimental planning must include the following aspects:

 Problem statement
 Variables
 Hypothesis
 List of apparatus and materials
 Experimental procedures
 Presentation of data
[17 marks]
KERTAS SOALAN TAMAT
Disediakan oleh : Disemak oleh : Disahkan oleh:
…………………................. ……………...…….............. ……...……...………...........

( CIK OOI HUEY LING ) ( EN. NG KIN KIT ) ( EN. LEE TUCK KEEN )
Guru Biologi Guru Kanan Sains Penolong Kanan
dan Matematik Pentadbiran

SULIT

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SULIT 2

1. Most fruits contain ascorbic acid normally known as vitamin C.

A group of students carried out an experiment to determine the concentration of vitamin C in

Diagram 1.1 Diagram 1.2

The students carried out the following steps:

Table 1 shows the result of the experiment.

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Inference from observation 2:

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Banana Apple Blackcurrant Pineapple

C6H12O6 + 6 O2 → 6 H2O + 6CO2

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Your experimental planning must include the following aspects:

KERTAS SOALAN TAMAT

Disediakan oleh : Disemak oleh : Disahkan oleh:

…………………................. ……………...…….............. ……...……...………...........

4551/3 [Lihat Sebelah]

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