Microbiology Lab MidTerms
Microbiology Lab MidTerms
Microbiology Lab MidTerms
MidTerms
B. Hydrolysis of Protein
1. Casein
o Main protein is milk
o Enzyme: Casease
o Zone of clearing round the colony: positive
result for hydrolysis of casein
o No zone of clearing: negative result
2. Gelatin
o Gegraded by gelatinase
C. Hydrolysis of Fats
Hydrolyzed by lipase
Fatty acid + Glycerol
Positive result using Tributylin Agaar: zone
of clearing around the colony
Positive Result for Spirit Blue Agar: zone of
clearing
Lipase is elaborated: positive result is
“irridescence”- play of colors around the
colonies.